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Articles 61 - 88 of 88
Full-Text Articles in Engineering
Potential Economic Impacts Of Allocating More Land For Bioenergy Biomass Production In Virginia, Thomas O. Ochuodho, Janaki R. R. Alavalapati, Pankaj Lal, Domena A. Agyeman, Bernabas Wolde, Pralhad Burli
Potential Economic Impacts Of Allocating More Land For Bioenergy Biomass Production In Virginia, Thomas O. Ochuodho, Janaki R. R. Alavalapati, Pankaj Lal, Domena A. Agyeman, Bernabas Wolde, Pralhad Burli
Forestry and Natural Resources Faculty Publications
The growing attention to renewable energy and rural development has created greater demand for production of biomass feedstock for bioenergy. However, forest growth rates and the amount of land in most existing forests may not be sufficient to sustainably supply the forest biomass required to support existing forest products industries and the expanding bioenergy industry. Additionally, concerns about agricultural land use competition have dampened expansion of biomass production on agricultural land base. One of the ways to meet the growing forest biomass feedstock demand for bioenergy production is by allocating currently marginal non-forested land for growing bioenergy feedstocks. In Virginia, …
Metabolomics Of Sorghum Roots During Nitrogen Stress Reveals Compromised Metabolic Capacity For Salicylic Acid Biosynthesis, Amy M. Sheflin, Dawn Chiniquy, Chaohui Yuan, Emily Goren, Indrajit Kumar, Max Braud, Thomas Brutnell, Andrea L. Eveland, Susannah Tringe, Peng Liu, Stephen Kresovich, Ellen Marsh, Daniel P. Schachtman, Jessica E. Prenni
Metabolomics Of Sorghum Roots During Nitrogen Stress Reveals Compromised Metabolic Capacity For Salicylic Acid Biosynthesis, Amy M. Sheflin, Dawn Chiniquy, Chaohui Yuan, Emily Goren, Indrajit Kumar, Max Braud, Thomas Brutnell, Andrea L. Eveland, Susannah Tringe, Peng Liu, Stephen Kresovich, Ellen Marsh, Daniel P. Schachtman, Jessica E. Prenni
Nebraska Center for Biotechnology: Faculty and Staff Publications
Sorghum (Sorghum bicolor [L.] Moench) is the fifth most productive cereal crop worldwide with some hybrids having high biomass yield traits making it promising for sustainable, economical biofuel production. To maximize biofuel feedstock yields, a more complete understanding of metabolic responses to low nitrogen (N) will be useful for incorporation in crop improvement efforts. In this study, 10 diverse sorghum entries (including inbreds and hybrids) were field-grown under low and full N conditions and roots were sampled at two time points for metabolomics and 16S amplicon sequencing. Roots of plants grown under low N showed altered metabolic profiles at …
Illumination Pattern Design With Deep Learning For Single-Shot Fourier Ptychographic Microscopy, Yi Fei Cheng , '21, Megan Strachan , '21, Zachary Weiss , '20, Moniher Deb , '19, Dawn M. Carone, Vidya Ganapati
Illumination Pattern Design With Deep Learning For Single-Shot Fourier Ptychographic Microscopy, Yi Fei Cheng , '21, Megan Strachan , '21, Zachary Weiss , '20, Moniher Deb , '19, Dawn M. Carone, Vidya Ganapati
Biology Faculty Works
Fourier ptychographic microscopy allows for the collection of images with a high space-bandwidth product at the cost of temporal resolution. In Fourier ptychographic microscopy, the light source of a conventional widefield microscope is replaced with a light-emitting diode (LED) matrix, and multiple images are collected with different LED illumination patterns. From these images, a higher-resolution image can be computationally reconstructed without sacrificing field-of-view. We use deep learning to achieve single-shot imaging without sacrificing the space-bandwidth product, reducing the acquisition time in Fourier ptychographic microscopy by a factor of 69. In our deep learning approach, a training dataset of high-resolution images …
The Small Rna Teg41 Regulates Expression Of The Alpha Phenol-Soluble Modulins And Is Required For Virulence In Staphylococcus Aureus, Rachel L. Zapf, Richard E. Wiemels, Rebecca A. Keogh, Donald L. Holzschu, Kayla M. Howell, Emily Trzeciak, Andrew R. Caillet, Kellie A. King, Samantha A. Selhorst, Michael J. Naldrett, Jeffrey L. Bose, Ronan K. Carroll
The Small Rna Teg41 Regulates Expression Of The Alpha Phenol-Soluble Modulins And Is Required For Virulence In Staphylococcus Aureus, Rachel L. Zapf, Richard E. Wiemels, Rebecca A. Keogh, Donald L. Holzschu, Kayla M. Howell, Emily Trzeciak, Andrew R. Caillet, Kellie A. King, Samantha A. Selhorst, Michael J. Naldrett, Jeffrey L. Bose, Ronan K. Carroll
Nebraska Center for Biotechnology: Faculty and Staff Publications
Small RNAs (sRNAs) remain an understudied class of regulatory molecules in bacteria in general and in Gram-positive bacteria in particular. In the major human pathogen Staphylococcus aureus, hundreds of sRNAs have been identified; however, only a few have been characterized in detail. In this study, we investigate the role of the sRNA Teg41 in S. aureus virulence. We demonstrate that Teg41, an sRNA divergently transcribed from the locus that encodes the cytolytic alpha phenolsoluble modulin (αPSM) peptides, plays a critical role in αPSM production. Overproduction of Teg41 leads to an increase in αPSM levels and a corresponding increase in …
Terpene Synthase Genes Originated From Bacteria Through Horizontal Gene Transfer Contribute To Terpenoid Diversity In Fungi, Qidong Jia, Xinlu Chen, Tobias G. Kollner, Jan Rinkel, Jianyu Fu, Jessy Labbe, Wangdan Xiong, Jeroen S. Dickschat, Jonathan Gershenzon, Feng Chen
Terpene Synthase Genes Originated From Bacteria Through Horizontal Gene Transfer Contribute To Terpenoid Diversity In Fungi, Qidong Jia, Xinlu Chen, Tobias G. Kollner, Jan Rinkel, Jianyu Fu, Jessy Labbe, Wangdan Xiong, Jeroen S. Dickschat, Jonathan Gershenzon, Feng Chen
Nebraska Center for Biotechnology: Faculty and Staff Publications
Fungi are successful eukaryotes of wide distribution. They are known as rich producers of secondary metabolites, especially terpenoids, which are important for fungi-environment interactions. Horizontal gene transfer (HGT) is an important mechanism contributing to genetic innovation of fungi. However, it remains unclear whether HGT has played a role in creating the enormous chemical diversity of fungal terpenoids. Here we report that fungi have acquired terpene synthase genes (TPSs), which encode pivotal enzymes for terpenoid biosynthesis, from bacteria through HGT. Phylogenetic analysis placed the majority of fungal and bacterial TPS genes from diverse taxa into two clades, indicating ancient …
Extraction Of Astaxanthin From Engineered Camelina Sativa Seed Using Ethanol-Modified Supercritical Carbon Dioxide, Liyang Xie, Edgar B. Cahoon, Yue Zhang, Ozan Ciftci
Extraction Of Astaxanthin From Engineered Camelina Sativa Seed Using Ethanol-Modified Supercritical Carbon Dioxide, Liyang Xie, Edgar B. Cahoon, Yue Zhang, Ozan Ciftci
Department of Food Science and Technology: Faculty Publications
Natural astaxanthin, a high-value carotenoid that is currently extracted mainly from marine organisms, was extracted from engineered camelina seed using ethanol-modified supercritical carbon dioxide (SC-CO2) for the first time, and compared with hexane and accelerated solvent extraction using hexane and ethanol. Response surface methodology (RSM) based on central composite rotatable design was employed to investigate the effect of pressure (30–45 MPa), temperature (40–60 °C), and ethanol concentration (10–35%, w/w). RSM-optimized conditions (41.6 MPa, 36.6 °C and 42.0% ethanol concentration) predicted the astaxanthin concentration as 437 μg/g oil, whereas the actual concentration was 421 ± 14 μg/g oil. Astaxanthin …
Cichlid Size Vs Flow Rate, Amy E. Naumovski
Cichlid Size Vs Flow Rate, Amy E. Naumovski
STAR Program Research Presentations
Cichlids are a diverse group of fish that are known for their prenatal and postnatal care of their eggs and frys (baby fish). This unique characteristic creates a limitation on the amount of eggs a cichlid can lay and protect, which is particulalry effected by the size of the eggs. Cichlids have a large variance in their egg size, which correlates to a large variance in their fry size. This project explores the trade offs cichlids make in egg size and fry size by specifically testing the ability of varying sized cichlids to swim in different flow rates. This could …
Human Islet Response To Selected Type 1 Diabetes-Associated Bacteria: A Transcriptome-Based Study, Ahmed M. Abdellatif, Heather Jensen Smith, Robert Z. Harms, Nora Sarvetnick
Human Islet Response To Selected Type 1 Diabetes-Associated Bacteria: A Transcriptome-Based Study, Ahmed M. Abdellatif, Heather Jensen Smith, Robert Z. Harms, Nora Sarvetnick
Journal Articles: Regenerative Medicine
Type 1 diabetes (T1D) is a chronic autoimmune disease that results from destruction of pancreatic β-cells. T1D subjects were recently shown to harbor distinct intestinal microbiome profiles. Based on these findings, the role of gut bacteria in T1D is being intensively investigated. The mechanism connecting intestinal microbial homeostasis with the development of T1D is unknown. Specific gut bacteria such as Bacteroides dorei (BD) and Ruminococcus gnavus (RG) show markedly increased abundance prior to the development of autoimmunity. One hypothesis is that these bacteria might traverse the damaged gut barrier, and their constituents elicit a response from human islets that causes …
Research Day Abstracts 2018-2019
Ua84 Sigma Chi, Wku Archives
Ua84 Sigma Chi, Wku Archives
WKU Archives Collection Inventories
Records created by and about the WKU chapter of Sigma Xi.
Appalachian Economic Futures, Dewayne Barton, John Deskins, Paul Corbit Brown, William Hal Gorby, Jill Moles Mullins, Nicholas F. Stump, Matt Winans, Brenden E. Mcneil, Eloise Elliott, Chris Haddox, E Gordon Gee, Eddie Brzostek, Audra Slocum, Trevor Mckenzie, Tom Hansell
Appalachian Economic Futures, Dewayne Barton, John Deskins, Paul Corbit Brown, William Hal Gorby, Jill Moles Mullins, Nicholas F. Stump, Matt Winans, Brenden E. Mcneil, Eloise Elliott, Chris Haddox, E Gordon Gee, Eddie Brzostek, Audra Slocum, Trevor Mckenzie, Tom Hansell
Exhibit Panels
When people talk about the future of Appalachia, they typically mean economic futures: What will happen to coal jobs? How will Appalachia diversify its economy? What about poverty? Stereotypes about our region represent our people as disinterested in education, and hostile to innovation, technology, and sustainability. We see a different story. This part of the exhibit looks at ways that Appalachians are taking their strong sense of culture, their linguistic distinctiveness, and their relationship to the natural world to imagine new futures in diverse industries, sustainable practices, and stronger education systems.
Nanosecond Pulsed Electric Signals Can Affect Electrostatic Environment Of Protiens Below The Threshold Of Conformational Effects: The Case Study Of Sod1 With A Molecular Simulation Study, Elena Della Valle, Paolo Marracino, Olga Pakhomova, Micaela Liberti, Francesca Apollonio
Nanosecond Pulsed Electric Signals Can Affect Electrostatic Environment Of Protiens Below The Threshold Of Conformational Effects: The Case Study Of Sod1 With A Molecular Simulation Study, Elena Della Valle, Paolo Marracino, Olga Pakhomova, Micaela Liberti, Francesca Apollonio
Bioelectrics Publications
Electric fields can be a powerful tool to interact with enzymes or proteins, with an intriguing perspective to allow protein manipulation. Recently, researchers have focused the interest on intracellular enzyme modifications triggered by the application of nanosecond pulsed electric fields. These findings were also supported by theoretical predictions from molecular dynamics simulations focussing on significant variations in protein secondary structures. In this work, a theoretical study utilizing molecular dynamics simulations is proposed to explore effects of electric fields of high intensity and very short nanosecond duration applied to the superoxide dismutase (Cu/Zn-SOD or SOD-1), an important enzyme involved in the …
Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori
Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori
Bioelectrics Publications
Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated the mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors. Our data show that in vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent cell death which was not accompanied by caspase activation or PARP cleavage. The lack of nsPEF-induced apoptosis was confirmed in vivo in B16F10 tumors. NsPEF also failed to trigger ICD-linked responses such …
Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori
Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori
Bioelectrics Publications
Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of …
Assessment Of Physicochemical Characteristics, Thermal Stability And Release Profile Of Ascorbic Acid Microcapsules Obtained By Complex Coacervation, Michele Cristina Rodrigues Da Cruz, João Luiz Andreotti Dagostin, Camila Augusto Perussello, Maria Lúcia Masson
Assessment Of Physicochemical Characteristics, Thermal Stability And Release Profile Of Ascorbic Acid Microcapsules Obtained By Complex Coacervation, Michele Cristina Rodrigues Da Cruz, João Luiz Andreotti Dagostin, Camila Augusto Perussello, Maria Lúcia Masson
Articles
The aim of this study was to determine the physicochemical characteristics, thermal stability and release profile of ascorbic acid (AA) microcapsules obtained by complex coacervation. Gelatin and gum arabic were used as wall materials in concentrations of 2.5, 5.0 and 7.5 g% (w/v). The coacervate microcapsules were freeze-dried and assessed for physicochemical characteristics, thermal behavior and stability during 60 days of storage. The release profile was evaluated at different pH values (1.1, 2.2, 5.4, 7.4, 9.6 and 12). The encapsulation efficiency ranged from 27.3 to 93.8%. The microcapsules of AA presented good characteristics for application in food matrices, such as …
Manipulation Of The Precursor Supply For High-Level Production Of Longifolene By Metabolically Engineered Escherichia Coli, Yujin Cao, Rubing Zhang, Wei Liu, Guang Zhao, Wei Niu, Jiantao Guo, Mo Xian, Huizhou Liu
Manipulation Of The Precursor Supply For High-Level Production Of Longifolene By Metabolically Engineered Escherichia Coli, Yujin Cao, Rubing Zhang, Wei Liu, Guang Zhao, Wei Niu, Jiantao Guo, Mo Xian, Huizhou Liu
Department of Chemical and Biomolecular Engineering: Faculty Publications
Longifolene is a naturally occurring tricyclic sesquiterpene widely used in many different fields. Up to now, this valuable terpene was mainly manufactured from the high-boiling fraction of certain pine resins. Microbial production can be a promising alternative to the extraction from natural plant sources. Here, we present the metabolic engineering strategy to assemble biosynthetic pathway for longifolene production in Escherichia coli. E. coli was rendered to produce longifolene by heterologously expressing a codon optimized longifolene synthase from Picea abies. Augmentation of the metabolic flux to farnesyl pyrophosphate (FPP) by different FPP synthases conferred a 1.8-fold increase in longifolene …
Delineating The Role Of Fanca In Glucose-Stimulated Insulin Secretion In Β Cells Through Its Protein Interactome, Dragana Lagundzin, Wen-Feng Hu, Henry C. H. Law, Kimiko L. Krieger, Fangfang Qiao, Emalie J. Clement, Andjela T. Drincic, Olgica Nedic, Michael J. Naldrett, Sophie Alvarez, Nicholas T. Woods
Delineating The Role Of Fanca In Glucose-Stimulated Insulin Secretion In Β Cells Through Its Protein Interactome, Dragana Lagundzin, Wen-Feng Hu, Henry C. H. Law, Kimiko L. Krieger, Fangfang Qiao, Emalie J. Clement, Andjela T. Drincic, Olgica Nedic, Michael J. Naldrett, Sophie Alvarez, Nicholas T. Woods
Nebraska Center for Biotechnology: Faculty and Staff Publications
Hyperinsulinemia affects 72% of Fanconi anemia (FA) patients and an additional 25% experience lowered glucose tolerance or frank diabetes. The underlying molecular mechanisms contributing to the dysfunction of FA pancreas β cells is unknown. Therefore, we sought to evaluate the functional role of FANCA, the most commonly mutated gene in FA, in glucosestimulated insulin secretion (GSIS). This study reveals that FANCA or FANCB knockdown impairs GSIS in human pancreas β cell line EndoC-βH3. To identify potential pathways by which FANCA might regulate GSIS, we employed a proteomics approach to identify FANCA protein interactions in EndoC-βH3 differentially regulated in response to …
Nanostructured Materials For Food Applications: Spectroscopy, Microscopy And Physical Properties, Shubham Sharma, Swana Jaiswal, Brendan Duffy, Amit Jaiswal
Nanostructured Materials For Food Applications: Spectroscopy, Microscopy And Physical Properties, Shubham Sharma, Swana Jaiswal, Brendan Duffy, Amit Jaiswal
Articles
Nanotechnology deals with the matter of atomic or molecular scale. Other factors that define the character of a nanoparticle are its physical and chemical properties, such as surface area, surface charge, hydrophobicity of the surface, the thermal stability of the nanoparticle, and its antimicrobial activity. A nanoparticle is usually characterized by using microscopic and spectroscopic techniques. Microscopic techniques are used to characterize the size, shape, and location of the nanoparticle by producing an image of the individual nanoparticle. Several techniques, such as scanning electron microscopy (SEM), transmission electron microscopy/high resolution transmission electron microscopy (TEM/HRTEM), atomic force microscopy (AFM) and scanning …
Selective Distant Electrostimulation By Synchronized Bipolar Nanosecond Pulses, Elena C. Gianulis, Maura Casciola, Carol Zhou, Enbo Yang, Shu Xiao, Andrei G. Pakhomov
Selective Distant Electrostimulation By Synchronized Bipolar Nanosecond Pulses, Elena C. Gianulis, Maura Casciola, Carol Zhou, Enbo Yang, Shu Xiao, Andrei G. Pakhomov
Bioelectrics Publications
A unique aspect of electrostimulation (ES) with nanosecond electric pulses (nsEP) is the inhibition of effects when the polarity is reversed. This bipolar cancellation feature makes bipolar nsEP less efficient at biostimulation than unipolar nsEP. We propose to minimize stimulation near pulse-delivering electrodes by applying bipolar nsEP, whereas the superposition of two phase-shifted bipolar nsEP from two independent sources yields a biologically-effective unipolar pulse remotely. This is accomplished by electrical compensation of all nsEP phases except the first one, resulting in the restoration of stimulation efficiency due to cancellation of bipolar cancellation (CANCAN-ES). We experimentally proved the CANCAN-ES paradigm by …
Intratumoral Delivery Of Plasmid Il12 Via Electroporation Leads To Regression Of Injected And Noninjected Tumors In Merkel Cell Carcinoma, Shailender Bhatia, Natalie V. Longino, Natalie J. Miller, Rima Kulikauskas, Jayasri G. Iyer, Dafina Ibrani, Astrid Blom, David R. Byrd, Upendra Parvathaneni, Christopher Twitty, Jean S. Campbell, Mai H. Le, Sharron Gargosky, Robert H. Pierce, Richard Heller, Adil Daud, Paul Nghiem
Intratumoral Delivery Of Plasmid Il12 Via Electroporation Leads To Regression Of Injected And Noninjected Tumors In Merkel Cell Carcinoma, Shailender Bhatia, Natalie V. Longino, Natalie J. Miller, Rima Kulikauskas, Jayasri G. Iyer, Dafina Ibrani, Astrid Blom, David R. Byrd, Upendra Parvathaneni, Christopher Twitty, Jean S. Campbell, Mai H. Le, Sharron Gargosky, Robert H. Pierce, Richard Heller, Adil Daud, Paul Nghiem
Bioelectrics Publications
Purpose: Interleukin-12 (IL12) promotes adaptive type I immunity and has demonstrated antitumor efficacy, but systemic administration leads to severe adverse events (AE), including death. This pilot trial investigated safety, efficacy, and immunologic activity of intratumoral delivery of IL12 plasmid DNA (tavo) via in vivo electroporation (i.t.-tavo-EP) in patients with Merkel cell carcinoma (MCC), an aggressive virus-associated skin cancer.
Experimental Design: Fifteen patients with MCC with superficial injectable tumor(s) received i.t.-tavo-EP on days 1, 5, and 8 of each cycle. Patients with locoregional MCC (cohort A, N = 3) received one cycle before definitive surgery in week 4. …
Near-Field Electrospinning Of A Polymer/Bioactive Glass Composite To Fabricate 3d Biomimetic Structures, Krishna C. R. Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, D. E. Day, Ming-Chuan Leu
Near-Field Electrospinning Of A Polymer/Bioactive Glass Composite To Fabricate 3d Biomimetic Structures, Krishna C. R. Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, D. E. Day, Ming-Chuan Leu
Biological Sciences Faculty Research & Creative Works
Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES technique. …
Kentucky Water Resources Research Institute, University Of Kentucky: Annual Technical Report 2018, Kentucky Water Resources Research Institute, University Of Kentucky
Kentucky Water Resources Research Institute, University Of Kentucky: Annual Technical Report 2018, Kentucky Water Resources Research Institute, University Of Kentucky
KWRRI Annual Technical Reports (USGS’s 104b Grant Program)
In 2018, KWRRI launched a new website, https://www.research.uky.edu/kentucky-water-resources-research-institute, to better meet the needs of its stakeholders. KWRRI also established a repository for KWRRI’s reports, https://uknowledge.uky.edu/kwrri/. The repository includes historical KWRRI reports, annual symposium proceedings, technical reports, and the Water Distribution System Research Database. During the reporting period, KWRRI produced two editions of its semi-annual newsletter (Spring 2018 and Winter 2019). The newsletter provides updates, facilitates information sharing, and increases communication among those in the Kentucky water community. The 2019 Kentucky Water Resources Annual Symposium was held on March 25, 2019, in Lexington, Kentucky. The day-long symposium, attended by …
Capital Efficiency For Development Stage Biotech-Based Firms: An Ipo Perspective, Mark J. Ahn, Amir Shaygan
Capital Efficiency For Development Stage Biotech-Based Firms: An Ipo Perspective, Mark J. Ahn, Amir Shaygan
Engineering and Technology Management Faculty Publications and Presentations
Access to multiple tranches of capital is critical for predominantly no revenue development stage biotech firms. While financing needs are monotonically increasing over multiple years in the product development approval cycle, the market for high risk, milestone driven biotech investment is significantly more volatile than the financial markets as a whole. In this paper, we analyzed the role and relative importance of global biotech IPOs, as well as other sources of capital such as strategic alliances, for research and development funding. We also explored and assessed the degree of mismatch between the access to capital, operational efficiencies, and how firms …
New Types Of Neutrosophic Set/Logic/Probability, Neutrosophic Over-/ Under-/ Off Set, Neutrosophic Refined Set, And Their Extension To Plithogenic Set/Logic/Probability, With Applications, Florentin Smarandache
New Types Of Neutrosophic Set/Logic/Probability, Neutrosophic Over-/ Under-/ Off Set, Neutrosophic Refined Set, And Their Extension To Plithogenic Set/Logic/Probability, With Applications, Florentin Smarandache
Branch Mathematics and Statistics Faculty and Staff Publications
This international collective massive book, edited by Florentin Smarandache, has 716 pages, and represents the special issue on neutrosophic environment at SYMMETRY international journal (Scopus, Web of Science, IF: 2.143). The book contains 37 published papers, by 73 authors and coauthors, from 13 countries from around the world, in alphabetical order: China, Cuba, Ecuador, Egypt, India, Jordan, Korea, Malaysia, Pakistan, Romania, Saudi Arabia, United Kingdom, and United States of America.
Effective Plant Discrimination Based On The Combination Of Local Binary Pattern Operators And Multiclass Support Vector Machine Methods, Vi N T Le, Beniamin Apopei, Kamal Alameh
Effective Plant Discrimination Based On The Combination Of Local Binary Pattern Operators And Multiclass Support Vector Machine Methods, Vi N T Le, Beniamin Apopei, Kamal Alameh
Research outputs 2014 to 2021
Accurate crop and weed discrimination plays a critical role in addressing the challenges of weed management in agriculture. The use of herbicides is currently the most common approach to weed control. However, herbicide resistant plants have long been recognised as a major concern due to the excessive use of herbicides. Effective weed detection techniques can reduce the cost of weed management and improve crop quality and yield. A computationally efficient and robust plant classification algorithm is developed and applied to the classification of three crops: Brassica napus (canola), Zea mays (maize/corn), and radish. The developed algorithm is based on the …
Microcontroller Based Granular Urea Application Attachment For Rice Transplanter, Md Towfiqur Rahman, Md Monjurul Alam, Md Mosharraf Hossain, Muhammad Rashed Al Mamun
Microcontroller Based Granular Urea Application Attachment For Rice Transplanter, Md Towfiqur Rahman, Md Monjurul Alam, Md Mosharraf Hossain, Muhammad Rashed Al Mamun
Biological Systems Engineering: Papers and Publications
Transplanting and fertilizer application for rice production in Bangladesh are tedious, time consuming and laborious task, and mostly done manually. Mechanical transplanting of rice becoming popular in the country in recent years and few machines have been developed for granular urea deep placement, however, having some limitations. Placing granular urea precisely along with rice transplanting, an attempt was under taken to design and fabricate an electronic control granular urea applicator to be attach with a 4-row walk behind type rice transplanter. Fabrication of the electronic granular urea applicator was done in the workshop of the Department of Farm Power and …
Walking Biomechanics And Energetics Of Individuals With A Visual Impairment: A Preliminary Report, Hunter J. Bennett, Kevin A. Valenzuela, Kristina Fleenor, Steven Morrison, Justin A. Haegele
Walking Biomechanics And Energetics Of Individuals With A Visual Impairment: A Preliminary Report, Hunter J. Bennett, Kevin A. Valenzuela, Kristina Fleenor, Steven Morrison, Justin A. Haegele
Human Movement Sciences & Special Education Faculty Publications
Purpose.
Although walking gait in sighted populations is well researched, few studies have investigated persons with visual impairments (VIs). Given the lack of physical activity in people with VIs, it is possible that reduced efficiency in walking could adversely affect activity. The purposes of this preliminary study were to (1) examine the biomechanics and energetics utilized during independent and guided walking in subjects with VIs, and (2) compare gait biomechanics between people with VIs and sighted controls.
Methods.
Three-dimensional motion capture and force platforms were used during independent and guided walking at self-selected speeds. Joint angles, moments, external work, and …
End-To-End Learning Via A Convolutional Neural Network For Cancer Cell Line Classification, Darlington A. Akogo, Xavier-Lewis Palmer
End-To-End Learning Via A Convolutional Neural Network For Cancer Cell Line Classification, Darlington A. Akogo, Xavier-Lewis Palmer
Electrical & Computer Engineering Faculty Publications
Purpose: Computer vision for automated analysis of cells and tissues usually include extracting features from images before analyzing such features via various machine learning and machine vision algorithms. The purpose of this work is to explore and demonstrate the ability of a Convolutional Neural Network (CNN) to classify cells pictured via brightfield microscopy without the need of any feature extraction, using a minimum of images, improving work-flows that involve cancer cell identification.
Design/methodology/approach: The methodology involved a quantitative measure of the performance of a Convolutional Neural Network in distinguishing between two cancer lines. In their approach, they trained, validated and …