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Articles 181 - 210 of 4190
Full-Text Articles in Engineering
Fabrication Of Al/Al2o3 Composite By Powder Metallurgy Method From Aluminum And Rice Husk Ash, C Dhadsanadhep, T Luangvaranunt, J Umeda, K Kondoh
Fabrication Of Al/Al2o3 Composite By Powder Metallurgy Method From Aluminum And Rice Husk Ash, C Dhadsanadhep, T Luangvaranunt, J Umeda, K Kondoh
Journal of Metals, Materials and Minerals
Fabrication of Al-4wt.%Cu /Alumina composite was reported. The method of fabrication was powder metallurgy by in-situ reaction to form alumina during fabrication. Starting materials were mixture of aluminum, copper, and silica powders. Silica is in form of rice husk ash. The mixture was cold compacted, and sintered at 650°C for 1 h. Hot forging was carried out to further consolidate the sintered billet, followed by 10-h heat treatment at temperatures between 590 to 650°C. Phase analysis by XRD confirmed that in-situ reaction between silica and aluminum powders occurred during heat treatment at temperatures above 590°C. The products of reaction were …
Application Of Parent-Child Uav Tasking For Wildfire Detection And Response, Stephen T. Kubik
Application Of Parent-Child Uav Tasking For Wildfire Detection And Response, Stephen T. Kubik
Master's Theses
In recent years, unmanned aerial vehicles (UAVs) have become a dominant force in the aerospace industry. Recent technological developments have moved these aircraft from remote operation roles to more active response missions. Of particular interest is the possibility of applying UAVs toward solving complex problems in long-endurance missions. Under that belief, the feasibility of utilizing UAVs for wildfire detection and response was investigated in a partnership that included NASA’s Aeronautics Research Mission Directorate and Science Mission Directorate, and the United States Forest Service. Under NASA’s Intelligent Mission Management (IMM) project, research was conducted to develop a mission architecture that would …
Experiments And Models For The Thermal Response Of Railroad Tapered-Roller Bearings, Constantine Tarawneh, Kevin D. Cole, Brent Wilson, Fadi Alnaimat
Experiments And Models For The Thermal Response Of Railroad Tapered-Roller Bearings, Constantine Tarawneh, Kevin D. Cole, Brent Wilson, Fadi Alnaimat
Mechanical Engineering Faculty Publications
The need to understand and quantify the heat transfer paths within tapered-roller bearings is of great importance in the railroad industry since bearing cup temperature measurement is the primary tool utilized in the removal of distressed bearings from service. Experiments were performed to acquire temperature histories at several locations on a stationary bearing subjected to heat sources imbedded in two rollers. The experimental results were compared to zero- and first-order thermal models to estimate overall heat transfer coefficients from the bearing. The results presented here can be used to explain some of the mechanisms that lead to overheating of tapered-roller …
Gene Order Phylogeny Of The Genus Prochlorococcus, Haiwei Luo, Jian Shi, William Arndt, Jijun Tang, Robert Friedman
Gene Order Phylogeny Of The Genus Prochlorococcus, Haiwei Luo, Jian Shi, William Arndt, Jijun Tang, Robert Friedman
Faculty Publications
Background
Using gene order as a phylogenetic character has the potential to resolve previously unresolved species relationships. This character was used to resolve the evolutionary history within the genus Prochlorococcus, a group of marine cyanobacteria.
Methodology/Principal Findings
Orthologous gene sets and their genomic positions were identified from 12 species of Prochlorococcus and 1 outgroup species of Synechococcus. From this data, inversion and breakpoint distance-based phylogenetic trees were computed by GRAPPA and FastME. Statistical support of the resulting topology was obtained by application of a 50% jackknife resampling technique. The result was consistent and congruent with nucleotide sequence-based and gene-content based …
A Special-Purpose Architecture For Solving The Breakpoint Median Problem, Jason D. Bakos, Panormitis E. Elenis
A Special-Purpose Architecture For Solving The Breakpoint Median Problem, Jason D. Bakos, Panormitis E. Elenis
Faculty Publications
In this paper, we describe the design for a co-processor for whole-genome phylogenetic reconstruction. Our current design performs a parallelized breakpoint median computation, which is an expensive component of the overall application. When implemented on a field-programmable gate array (FPGA), our hardware breakpoint median achieves a maximum speedup of 1005times over software. When the coprocessor is used to accelerate the entire reconstruction procedure, we achieve a maximum application speedup of 417times. The results in this paper suggest that FPGA-based acceleration is a promising approach for computationally expensive phylogenetic problems, in spite of the fact that the involved algorithms are based …
A Membrane-Type Humidifier For Fuel Cell Applications: Controller Design, Analysis And Implementation, Denise A. Mckay, Anna G. Stefanopoulou, Jeffrey Cook
A Membrane-Type Humidifier For Fuel Cell Applications: Controller Design, Analysis And Implementation, Denise A. Mckay, Anna G. Stefanopoulou, Jeffrey Cook
Engineering: Faculty Publications
A membrane-based gas humidification apparatus was employed to actively manage the water vapor entrained in the reactant gas supplied to a fuel cell stack. The humidification system utilizes a gas bypass and a series of heaters to achieve accurate and fast humidity and temperature control. A change in fuel cell load induces a reactant mass flow rate disturbance to this humidification system. If not well regulated, this disturbance creates undesirable condensation and evaporation dynamics, both to the humidification system and the fuel cell stack. Therefore, controllers were devised, tuned and employed for temperature reference tracking and disturbance rejection. The coordination …
Investigation Of The Effective Use Of Warning Lights On Indiana Department Of Transportation (Indot) Vehicles And Equipment, Bob Mccullouch, Brandon Stevens
Investigation Of The Effective Use Of Warning Lights On Indiana Department Of Transportation (Indot) Vehicles And Equipment, Bob Mccullouch, Brandon Stevens
JTRP Technical Reports
This study was requested by INDOT to determine if current lighting packages used on INDOT maintenance vehicles, specifically snow plow vehicles, can be improved with other commercially available products. Different light products were obtained from various vendors, mounted on INDOT vehicles, and visual comparisons were performed under various lighting and weather conditions by a team of observers. The comparison evaluations were performed revealing that other light packages, LED models for most options, were brighter and certain colors more visible. The preferred light models are recommended. These recommendations were forwarded to appropriate individuals that make these decisions at INDOT. Implementation is …
Alternative Land Use Patterns To Minimize Congestion (Volume 3: Empirical Study Of Mixed Land Use Traditional Neighborhoods), Li Jin, Jon D. Fricker
Alternative Land Use Patterns To Minimize Congestion (Volume 3: Empirical Study Of Mixed Land Use Traditional Neighborhoods), Li Jin, Jon D. Fricker
JTRP Technical Reports
Urban sprawl creates serious traffic congestion. Alternative land use patterns may be the best solution. New Urbanists claim that, by placing frequently-visited sites within walking distance of homes and creating a pleasant walking environment, people are more willing to choose non-motorized transportation mode to do such activities.
Part I of this study investigated the ability of travel demand models to estimate the impacts of alternative land use patterns. Part II conducted an economic viability analysis for a mixed land use neighborhood and collected land use preferences at meetings of neighborhood associations. The objective in Part III was to evaluate the …
Use Of Micropiles For Foundations Of Transportation Structures Final Report, Hoyoung Seo, Monica Prezzi
Use Of Micropiles For Foundations Of Transportation Structures Final Report, Hoyoung Seo, Monica Prezzi
JTRP Technical Reports
In pile design, piles must be able to sustain axial loads from the superstructure without bearing capacity failure or structural damage. In addition, piles must not settle or deflect excessively in order for the serviceability of the superstructures to be maintained. In general, settlement controls the design of piles in most cases because, by the time a pile has failed in terms of bearing capacity, it is very likely that serviceability will have already been compromised. Therefore, realistic estimation of settlement for a given load is very important in design of axially loaded piles. This notwithstanding, pile design has relied …
A Novel Method For Characterization Of Peripheral Nerve Fiber Size Distributions By Group Delay, Robert B. Szlavik
A Novel Method For Characterization Of Peripheral Nerve Fiber Size Distributions By Group Delay, Robert B. Szlavik
Biomedical Engineering
The ability to determine the characteristics of peripheral nerve fiber size distributions would provide additional information to clinicians for the diagnosis of specific pathologies of the peripheral nervous system. Investigation of these conditions, using electrodiagnostic techniques, is advantageous in the sense that such techniques tend to be minimally invasive yet provide valuable diagnostic information. One of the principal electrodiagnostic tools available to the clinician is the nerve conduction velocity test. While the peripheral nerve conduction velocity test can provide useful information to the clinician regarding the viability of the nerve under study, it is a single-parameter test that yields no …
A Three-Dimensional Pattern-Space Representation For Volumetric Arrays, William C. Barott, Paul G. Steffes
A Three-Dimensional Pattern-Space Representation For Volumetric Arrays, William C. Barott, Paul G. Steffes
Publications
A three-dimensional pattern-space representation is presented for volumetric arrays. In this representation, the radiation pattern of an array is formed by the evaluation of the three-dimensional pattern-space on a spherical surface. The scan angle of the array determines the position of this surface within the pattern-space. This pattern-space representation is used in conjunction with a genetic algorithm to minimize the sidelobe levels exhibited by a thinned volumetric array during scanning.
Neural Network Output Feedback Control Of A Quadrotor Uav, Jagannathan Sarangapani, Travis Alan Dierks
Neural Network Output Feedback Control Of A Quadrotor Uav, Jagannathan Sarangapani, Travis Alan Dierks
Electrical and Computer Engineering Faculty Research & Creative Works
A neural network (NN) based output feedback controller for a quadrotor unmanned aerial vehicle (UAV) is proposed. The NNs are utilized in the observer and for generating virtual and actual control inputs, respectively, where the NNs learn the nonlinear dynamics of the UAV online including uncertain nonlinear terms like aerodynamic friction and blade flapping. It is shown using Lyapunov theory that the position, orientation, and velocity tracking errors, the virtual control and observer estimation errors, and the NN weight estimation errors for each NN are all semi-globally uniformly ultimately bounded (SGUUB) in the presence of bounded disturbances and NN functional …
Neural-Network-Based State Feedback Control Of A Nonlinear Discrete-Time System In Nonstrict Feedback Form, Pingan He, Jagannathan Sarangapani
Neural-Network-Based State Feedback Control Of A Nonlinear Discrete-Time System In Nonstrict Feedback Form, Pingan He, Jagannathan Sarangapani
Electrical and Computer Engineering Faculty Research & Creative Works
In this paper, a suite of adaptive neural network (NN) controllers is designed to deliver a desired tracking performance for the control of an unknown, second-order, nonlinear discrete-time system expressed in nonstrict feedback form. In the first approach, two feedforward NNs are employed in the controller with tracking error as the feedback variable whereas in the adaptive critic NN architecture, three feedforward NNs are used. In the adaptive critic architecture, two action NNs produce virtual and actual control inputs, respectively, whereas the third critic NN approximates certain strategic utility function and its output is employed for tuning action NN weights …
A Circuit Model For Esd Performance Analysis Of Printed Circuit Boards, Byong-Su Seol, Jong-Sung Lee, Jae-Deok Lim, Hyungseok Lee, Harkbyeong Park, Argha Nandy, David Pommerenke
A Circuit Model For Esd Performance Analysis Of Printed Circuit Boards, Byong-Su Seol, Jong-Sung Lee, Jae-Deok Lim, Hyungseok Lee, Harkbyeong Park, Argha Nandy, David Pommerenke
Electrical and Computer Engineering Faculty Research & Creative Works
This paper provides a SPICE-compatible circuit model for characterizing electrostatic discharge (ESD) clamping performance of protection devices mounted on printed circuit boards (PCBs). An equivalent circuit model for a commercial ESD generator is introduced and a simulation methodology of an ESD protection device with non-linear resistance characteristic using voltage controlled current source is described. These models combined to create a full circuit model with a PCB model in a SPICE-like circuit simulator. Comparison results between the simulated and measured are presented to verify the accuracy of the proposed circuit model. A trade-off analysis between the ESD clamping performance and signal …
On Nonparametric Residual Variance Estimation, Elia Liitiäinen, Francesco Corona, Amaury Lendasse
On Nonparametric Residual Variance Estimation, Elia Liitiäinen, Francesco Corona, Amaury Lendasse
Engineering Management and Systems Engineering Faculty Research & Creative Works
In This Paper, the Problem of Residual Variance Estimation is Examined. the Problem is Analyzed in a General Setting Which Covers Non-Additive Heteroscedastic Noise under Non-Iid Sampling. to Address the Estimation Problem, We Suggest a Method based on Nearest Neighbor Graphs and We Discuss its Convergence Properties under the Assumption of a Hölder Continuous Regression Function. the Universality of the Estimator Makes It an Ideal Tool in Problems with Only Little Prior Knowledge Available. © 2008 Springer Science business Media, LLC.
Improvements In Steel Melting Efficiency -- Industrial Trials, Kent D. Peaslee, Semen Naumovich Lekakh, Edith Martinez
Improvements In Steel Melting Efficiency -- Industrial Trials, Kent D. Peaslee, Semen Naumovich Lekakh, Edith Martinez
Materials Science and Engineering Faculty Research & Creative Works
Industrial trials were completed to improve energy efficiency in steel melting. First, the benefits of increased chemical energy from an oxyfuel burner and a Co-Jet system in a basic 20 ton electric arc furnace (EAF) were studied. Observations and measurements were made during production before and after the installation of the two systems. The additional chemical energy improved energy efficiency and resulted in increased production. In addition, production using a basic EAF practice was compared to the traditional acid EAF practice. Second, an industrial trial using a 750 lb ladle with a combination of a lightweight alumina castable refractory and …
Energy Efficiency In Steel Casting Production, Raymond Monroe, Kent D. Peaslee, R. Eppich
Energy Efficiency In Steel Casting Production, Raymond Monroe, Kent D. Peaslee, R. Eppich
Materials Science and Engineering Faculty Research & Creative Works
No abstract provided.
Using Automated Inclusion Analysis For Casting Process Improvements, Vintee Singh, Semen Naumovich Lekakh, Kent D. Peaslee
Using Automated Inclusion Analysis For Casting Process Improvements, Vintee Singh, Semen Naumovich Lekakh, Kent D. Peaslee
Materials Science and Engineering Faculty Research & Creative Works
Different industrial melting and ladle practices (deoxidation, slag, refractory types, etc.) used in steel foundries were analyzed and compared using an ASPEX automated inclusion analyzer for study of inclusions. The effects of deoxidation and pouring practices on the size, type and number of inclusions were evaluated for steel foundries equipped with induction and arc melting furnaces, with capacities ranging from 1 to 20 tons. Samples were collected from the furnace, ladle, and castings. Specific rules were developed for classification of inclusions by composition, size distribution and shape. Inclusion statistics, including composition, quantity, shape, and size during cast steel processing from …
A Retrospective On Twenty Years Of The Design Theory And Methodology Conference, Jacquelyn K. Stroble, Robert L. Nagel, Kerry R. Poppa, Matt Robert Bohm, Robert B. Stone
A Retrospective On Twenty Years Of The Design Theory And Methodology Conference, Jacquelyn K. Stroble, Robert L. Nagel, Kerry R. Poppa, Matt Robert Bohm, Robert B. Stone
Mechanical and Aerospace Engineering Faculty Research & Creative Works
Since its birth from the Design Automation Conference (DAC) and the Association for the Advancement of Artificial Intelligence (AAAI) twenty years ago, the Design Theory and Methodology (DTM) Conference has accepted 769 papers for presentation in a total of 179 tracks. Papers have covered advances in design theory and methods as well as design education, decision making, product development, collaborative endeavors, case studies, information processing, computational methods and industrial applications. through the years tracks have evolved to better define existing research topics and branched to spawn new areas of interest. This paper presents a retrospective of the past twenty years …
An Empirical Study Of Performance Benefits Of Network Coding In Multihop Wireless Networks, Y. Charlie Hu, Chih-Chun Wang
An Empirical Study Of Performance Benefits Of Network Coding In Multihop Wireless Networks, Y. Charlie Hu, Chih-Chun Wang
Department of Electrical and Computer Engineering Technical Reports
Recently, network coding has gained much popularity and several practical routing schemes have been proposed for wireless mesh networks that exploit interflow network coding for improved throughput. However, the evaluation of these protocols either assumed simple topologies and traffic patterns such as opposite flows along a single chain, or small, dense networks which have ample overhearing of each other’s transmissions in addition to many overlapping flows. In this paper, we seek to answer the fundamental question: how much performance benefit from network coding can be expected for general traffic patterns in a moderate-sized wireless mesh network? We approach this question …
Alternative Land Use Patterns To Minimize Congestion (Volume 1: Comparative Analysis Of Mixed Land Use And Separated Land Use Neighborhoods), Amica Bose, Jon D. Fricker
Alternative Land Use Patterns To Minimize Congestion (Volume 1: Comparative Analysis Of Mixed Land Use And Separated Land Use Neighborhoods), Amica Bose, Jon D. Fricker
JTRP Technical Reports
Urban sprawl creates serious traffic congestion. Alternative land use patterns may be the best solution. New Urbanists claim that, by placing frequently-visited sites within walking distance of homes and creating a pleasant walking environment, people are more willing to choose non-motorized transportation mode to do such activities.
Part I of this study investigated the ability of travel demand models to estimate the impacts of alternative land use patterns. Part II conducted an economic viability analysis for a mixed land use neighborhood and collected land use preferences at meetings of neighborhood associations. The objective in Part III was to evaluate the …
Experiments And Models For The Thermal Response Of Railroad Tapered-Roller Bearings, Constantine M. Tarawneh, Kevin D. Cole, Brent M. Wilson, Fadi Alnaimat
Experiments And Models For The Thermal Response Of Railroad Tapered-Roller Bearings, Constantine M. Tarawneh, Kevin D. Cole, Brent M. Wilson, Fadi Alnaimat
Department of Mechanical and Materials Engineering: Faculty Publications
The need to understand and quantify the heat transfer paths within tapered-roller bearings is of great importance in the railroad industry since bearing cup temperature measurement is the primary tool utilized in the removal of distressed bearings from service. Experiments were performed to acquire temperature histories at several locations on a stationary bearing subjected to heat sources imbedded in two rollers. The experimental results were compared to zero- and first-order thermal models to estimate overall heat transfer coefficients from the bearing. The results presented here can be used to explain some of the mechanisms that lead to overheating of tapered-roller …
A Fundamental Study Of The Pcr Amplification Of Gc-Rich Dna Templates, T. G. Mamedov, Elsje Pienaar, Scott E. Whitney, Joel R. Termaat, G. Carvill, R. Goliath, Anuradha Subramanian, Hendrik J. Viljoen
A Fundamental Study Of The Pcr Amplification Of Gc-Rich Dna Templates, T. G. Mamedov, Elsje Pienaar, Scott E. Whitney, Joel R. Termaat, G. Carvill, R. Goliath, Anuradha Subramanian, Hendrik J. Viljoen
Hendrik J. Viljoen Publications
A theoretical analysis is presented with experimental confirmation to conclusively demonstrate the critical role that annealing plays in efficient PCR amplification of GC-rich templates. The analysis is focused on the annealing of primers at alternative binding sites (competitive annealing) and the main result is a quantitative expression of the efficiency (η) of annealing as a function of temperature (TA), annealing period (tA), and template composition. The optimal efficiency lies in a narrow region of TA and tA for GC-rich templates and a much broader region for normal GC templates. To confirm the …
Monitoring Tissue Engineering Using Magnetic Resonance Imaging, Huihui Xu, Shadi F. Othman, Richard L. Magin
Monitoring Tissue Engineering Using Magnetic Resonance Imaging, Huihui Xu, Shadi F. Othman, Richard L. Magin
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Assessment of tissue regeneration is essential to optimize the stages of tissue engineering (cell proliferation, tissue development and implantation). Optical and X-ray imaging have been used in tissue engineering to provide useful information, but each has limitations: for example, poor depth penetration and radiation damage. Magnetic resonance imaging (MRI) largely overcomes these restrictions, exhibits high resolution (approximately 100 μm) and can be applied both in vitro and in vivo. Recently, MRI has been used in tissue engineering to generate spatial maps of tissue relaxation times (T1, T2), water diffusion coefficients, and the stiffness (shear moduli) of …
Quantitative Rrna-Targeted Solution-Based Hybridization Assay Using Peptide Nucleic Acid Molecular Beacons, Xu Li, Eberhard Morgenroth, Lutgarde Raskin
Quantitative Rrna-Targeted Solution-Based Hybridization Assay Using Peptide Nucleic Acid Molecular Beacons, Xu Li, Eberhard Morgenroth, Lutgarde Raskin
Department of Civil and Environmental Engineering: Faculty Publications
The potential of a solution-based hybridization assay using peptide nucleic acid (PNA) molecular beacon (MB) probes to quantify 16S rRNA of specific populations in RNA extracts of environmental samples was evaluated by designing PNA MB probes for the genera Dechloromonas and Dechlorosoma. In a kinetic study with 16S rRNA from pure cultures, the hybridization of PNA MB to target 16S rRNA exhibited a higher final hybridization signal and a lower apparent rate constant than the hybridizations to nontarget 16S rRNAs. A concentration of 10 mM NaCl in the hybridization buffer was found to be optimal for maximizing the difference between …
Using Archived Its Data To Measure The Operational Benefits Of A System-Wide Adaptive Ramp Metering System, Robert L. Bertini, Christopher Monsere, Oren Eshel, Soyoung Ahn
Using Archived Its Data To Measure The Operational Benefits Of A System-Wide Adaptive Ramp Metering System, Robert L. Bertini, Christopher Monsere, Oren Eshel, Soyoung Ahn
Civil and Environmental Engineering Faculty Publications and Presentations
A System-Wide Adaptive Ramp Metering (SWARM) system has been implemented in the Portland, Oregon metropolitan area, replacing the previous pre-timed ramp-metering system that had been in operation since 1981. SWARM has been deployed on six major corridors and operates during the morning and afternoon peak hours. This report presents results of a "before" and "after" evaluation of the performance of two freeway corridors as part of ongoing efforts to measure the benefits of the new SWARM system, as compared to the pre-timed system. The study benefited from using the existing regional data, surveillance and communications infrastructure in addition to a …
Determination Of Vibrational Energy Levels And Transition Dipole Moments Of Co2 Molecules By Density Functional Theory, Zhi Liang, Hai-Lung Tsai
Determination Of Vibrational Energy Levels And Transition Dipole Moments Of Co2 Molecules By Density Functional Theory, Zhi Liang, Hai-Lung Tsai
Mechanical and Aerospace Engineering Faculty Research & Creative Works
An Efficient Method is Presented to Calculate the Intra-Molecular Potential Energies and Electrical Dipole Moments of CO2 Molecules at the Electronic Ground State by Solving the Kohn-Sham (KS) Equation for a Total of 101 992 Nuclear Configurations. the Projector-Augmented Wave (PAW) Exchange-Correlation Potential Functionals and Plane Wave (PW) Basis Functions Were Used in Solving the KS Equation. the Calculated Intra-Molecular Potential Function Was Then Included in the Pure Vibrational Schrödinger Equation to Determine the Vibrational Energy Eigen Values and Eigen Functions. the Vibrational Wave Functions Combined with the Calculated Dipole Moment Function Were Used to Determine the Transition Dipole Moments. …
Optimal Re-Entry Trajectory Terminal State Due To Variations In Waypoint Locations, William J. Karasz
Optimal Re-Entry Trajectory Terminal State Due To Variations In Waypoint Locations, William J. Karasz
Theses and Dissertations
The Air Force's Prompt Global Reach concept describes the desire to have a capability to reach any target within a 9000 nautical mile radius within two hours of launch. To meet this objective, much effort is being devoted to hypersonics and re-entry vehicles. Given the limited maneuverability of hypersonic vehicles, computational modeling is used to generate trajectories before launch to strike intended targets. In addition to endpoint (target) constraints, additional waypoints may constrain the trajectory. This research finds the optimal trajectory which satisfies the endpoint and waypoint constraints, and then investigates where else the vehicle can go while still meeting …
Application Of Structural Control For Civil Engineering Structures, Abdollah Shafieezadeh
Application Of Structural Control For Civil Engineering Structures, Abdollah Shafieezadeh
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study presents the application of control methods in seismic mitigation of structural responses. The study consists of two parts. In the first section, fractional order filters are utilized to enhance the performance of the conventional LQR method for optimal robust control of a simple civil structure. The introduced filters modify the state variables fed back to the constant gain controller. Four combinations of fractional order filter and LQR are considered and optimized based on a new performance criterion defined in the paper. Introducing fractional order filters is shown to improve the results considerably for both the artificially generated ground …
Gene Expression Analysis Of Immobilized Saccharomyces Cerevisiae, Ryan Michael Summers
Gene Expression Analysis Of Immobilized Saccharomyces Cerevisiae, Ryan Michael Summers
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Immobilization is an effective method to increase ethanol production, as proven by previous research. Results almost exclusively demonstrate an increase in ethanol production by and decrease in reproduction rate of immobilized Saccharomyces cerevisiae cells. Recently, research has been conducted to determine the cause of this change. The extreme variance in results due to lack of technology makes it difficult to determine the cellular changes induced by immobilization. With the advent of new technology, specifically gene expression analysis, the RNA content of cells can be easily and rapidly analyzed.
S. cerevisiae cells were immobilized in 3% (w/v) calcium alginate beads and …