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Articles 1051 - 1080 of 1409
Full-Text Articles in Engineering
Dielectric And Optical Study Of Biaxial Bent-Core Nematic Liquid Crystal, S. P. Sreenilayam, M. Nagaraj, Yuri Panarin, J. K. Vij, C. Keith, C. Tschierske
Dielectric And Optical Study Of Biaxial Bent-Core Nematic Liquid Crystal, S. P. Sreenilayam, M. Nagaraj, Yuri Panarin, J. K. Vij, C. Keith, C. Tschierske
Articles
We report the observation of biaxial nematic phase in a bent-core molecular system in which the biaxiality exists on a microscopic scale using polarizing microscopy, electro-optics and dielectric spectroscopy. An application of electric field induces a macroscopic biaxiality and therefore electro-optic switching. The observed electro-optic switching is explained in terms of the interaction of the ferroelectric clusters with the electric field. Dielectric spectra of the sample shows existence of low-frequency collective processes related to the microscopic polar clusters. The dielectric constant changes its sign from positive to negative and again to positive values.
What Difference Does It Make?- The Current Role And Potential Impact Social Enterprise Can Play In The Regeneration Of Disadvantaged Communities, Gerard Doyle
Articles
At European level there is no universally accepted definition of what constitutes a social enterprise (GHK, 2006). However, the number of definitions of what constitutes a social enterprise reflects the diverse understanding of what a social enterprise actually is. The Department of Trade and Industry (DTI) (2002) definition is widely used: A social enterprise is a business with primarily social objectives whose surpluses are principally reinvested for that purpose in the business or in the community, rather than being driven by the need to maximise profit for shareholders and owners. Social enterprises share a number of common characteristics: they are …
Single Mode Optical Fiber Based Refractive Index Sensor Using Etched Cladding, Ajay Kumar, Geeta Gupta, Arun Mallik, Anuj Bhatnagar
Single Mode Optical Fiber Based Refractive Index Sensor Using Etched Cladding, Ajay Kumar, Geeta Gupta, Arun Mallik, Anuj Bhatnagar
Articles
The use of optical fiber for sensor applications is a topic of current interest. We report the fabrication of etched single mode optical fiber based refractive index sensor. Experiments are performed to determine the etch rate of fiber in buffered hydrofluoric acid, which can be high or low depending upon the temperature at which etching is carried out. Controlled wet etching of fiber cladding is performed using these measurements and etched fiber region is tested for refractive index sensing application. We report significant optical power change in fiber sensor at 1550 nm wavelength, in the presence of IPAsolution and also …
Successful Engineering And Technology Student Mobility: Key Student Perspectives And Quality Determinants Before, During And After Student Exchange Under The Atlantis Programme, Mike Murphy, Donal Mchale, Michael Dyrenfurth, James Barnes
Successful Engineering And Technology Student Mobility: Key Student Perspectives And Quality Determinants Before, During And After Student Exchange Under The Atlantis Programme, Mike Murphy, Donal Mchale, Michael Dyrenfurth, James Barnes
Articles
The EU-US Agreement through the Atlantis Programmes supports consortia of higher education and training institutions working together at undergraduate or graduate level to improve their educational services, to compare and modernise curricula and to develop joint study programmes with full recognition of credits and qualifications. The EU-US Atlantis Programme funds innovative projects across three strands: mobility projects, double or joint “transatlantic degrees” for students in the EU and US and policy-oriented measures. The main focus of activities must be on transatlantic rather than intra-European or intra-American interactions. Funded activities, such as the development of curricula, joint study programmes, exchanges and …
Critical Issues And Lessons Learned In Establishing Concurrent International Ms Degree Programs In Engineering Technology, Mike Murphy, Michael Dyrenfurth, Gary Bertoline, Robert Herrick, Kathryne Newton, Sancho Maria-Ribera, Nuria Castell, James Barnes, Matthias Kuder, Gareth O'Donnell
Critical Issues And Lessons Learned In Establishing Concurrent International Ms Degree Programs In Engineering Technology, Mike Murphy, Michael Dyrenfurth, Gary Bertoline, Robert Herrick, Kathryne Newton, Sancho Maria-Ribera, Nuria Castell, James Barnes, Matthias Kuder, Gareth O'Donnell
Articles
Globalization, competitiveness, and innovation are frequently employed themes as governments, business and industry and universities attempt to respond to the challenges facing them. Clearly business as usual is not likely to be successful in the future. One strategic response for universities has been a significant impetus – in many parts of the world – towards dual, joint or concurrent degree programs involving international partners. It is perhaps not surprising that engineering is among the disciplines that make most use of international collaboration but it seems that engineering technology programs do not yet participate as extensively in this aspect of international …
In-Silico Hyperthermia Performance Of A Near-Field Patch Antenna At Various Positions On A Human Body Model, Sergio Curto, Terrence See, Patrick Mcevoy, Max Ammann, Zhi Ning Chen
In-Silico Hyperthermia Performance Of A Near-Field Patch Antenna At Various Positions On A Human Body Model, Sergio Curto, Terrence See, Patrick Mcevoy, Max Ammann, Zhi Ning Chen
Articles
A compact patch applicator designed to enhance targeted energy coupling at 434 MHz is a key enabler for sensitizing temperature increments in body regions containing superficial tumours. A detailed FDTD body model is used to explore simulated RF coupling and temperature increments for typical clinical conditions. The antenna impedance matching, specific absorption rate and thermal distribution parameters are evaluated to identify applied performance outcomes. The analysis reveals physiological-RF coupling patterns for an optimised closely-coupled single element applicator.
Modeling Same-Direction Two-Lane Traffic For Bridge Loading, Eugene J. Obrien, Bernard Enright
Modeling Same-Direction Two-Lane Traffic For Bridge Loading, Eugene J. Obrien, Bernard Enright
Articles
Many highway bridges carry traffic in two same-direction lanes, and modeling the traffic loading on such bridges has been the subject of numerous studies. Different assumptions have been used to model multiple-presence loading events, particularly those featuring one truck in each lane. Using a database of weigh-in-motion measurements collected at two European sites for over 1 million trucks, this paper examines the relationships between adjacent vehicles in both lanes in terms of vehicle weights, speeds and inter-vehicle gaps. It is shown that there are various patterns of correlation, some of which are significant for bridge loading. A novel approach to …
A High Sensitivity, Evanescent Feld Refractometric Sensor Based On Tapered Multimode Fiber Interference, Pengfei Wang, Gilberto Brambilla, Ming Ding, Yuliya Semenova, Qiang Wu, Gerald Farrell
A High Sensitivity, Evanescent Feld Refractometric Sensor Based On Tapered Multimode Fiber Interference, Pengfei Wang, Gilberto Brambilla, Ming Ding, Yuliya Semenova, Qiang Wu, Gerald Farrell
Articles
We propose and experimentally demonstrate an enhanced evanescent field fiber refractometer based on a tapered multimode fiber sandwiched between two single-mode fibers. Experiments show that this fiber sensor offers ultrahigh sensitivity [better than 1900 nm/RIU at a refractive index (RI) of 1.44] for RI measurements within the range of 1.33–1.44 , in agreement with the theoretical predictions. This is the highest value reported to date (to our knowledge) in the literature.
Investigation And Experimental Measurement Of Scissor Blade Cutting Forces Using Fiber Bragg Grating Sensors., Dean Callaghan, Ginu Rajan, Mark Mcgrath, Eugene Dermot Coyle Professor, Yuliya Semenova, Gerald Farrell
Investigation And Experimental Measurement Of Scissor Blade Cutting Forces Using Fiber Bragg Grating Sensors., Dean Callaghan, Ginu Rajan, Mark Mcgrath, Eugene Dermot Coyle Professor, Yuliya Semenova, Gerald Farrell
Articles
This paper investigated the application of FBG sensors in the measurement of scissor blade–tissue interaction forces. Sensor placement at the blade–tissue interaction site provides the basis for increased force measurement accuracy, without compromising the functionality of the instrument. This increased accuracy provided the foundation for in-depth analysis of the force components generated during typical scissor cutting cycles. Theoretical analysis and experimental investigation explored the decoupling of the major forces present during cutting. The isolation of tissue-fracture forces from inter-blade friction forces was carried out. This is useful as knowledge of the fracture properties of biological tissues can be diff cult …
Formation Of Infrared Windows Of Transparency In One-Dimensional Silicon Photonic Crystals, Anna Baldycheva, Vladmir A; Tolmachev, Tatiana S. Perova, Kevin Berwick
Formation Of Infrared Windows Of Transparency In One-Dimensional Silicon Photonic Crystals, Anna Baldycheva, Vladmir A; Tolmachev, Tatiana S. Perova, Kevin Berwick
Articles
The optical properties of three-component one-dimensional photonic crystal (1-D PC) structures were investigated by modeling them as two-component PCs with an additional regular t-layer, using the gap map approach and the transfer matrix method. Numerical results demonstrate that the introduction of the t-layer affects the properties of the high-order photonic band gaps, replacing them with transmission bands and creating regions of transparency over certain wavelength ranges. For the first time, a map of the transmission bands for a three-component 1-D PC was generated. This map constitutes a unique design tool for infrared optical filters for silicon-based photonic integrated …
The Influence Of Thermal Expansion Of A Composite Material On Embedded Polarimetric Sensors, Manjusha Ramakrishnan, Ginu Rajan, Yuliya Semenova, Piotr Lesiak, Andrzej Domanski, Tomasz Wolinksi, Anna Boczkowska, Gerald Farrell
The Influence Of Thermal Expansion Of A Composite Material On Embedded Polarimetric Sensors, Manjusha Ramakrishnan, Ginu Rajan, Yuliya Semenova, Piotr Lesiak, Andrzej Domanski, Tomasz Wolinksi, Anna Boczkowska, Gerald Farrell
Articles
Abstract. Some of the most critical issues of the influence of the thermal expansion of composite materials on embedded polarimetric sensors for measurements of strain and temperature are studied in this paper. A composite material sample with polarimetric fiber sensors embedded in two distinct layers of a multi-layer composite structure is fabricated and characterized. The polarimetric fiber sensors used in this study are based on panda type fiber and polarization maintaining photonic crystal fiber (PM-PCF). The temperature sensitivities of polarimetric fiber sensors with acrylate buffer coated and buffer stripped polarization maintaining optical fibers are measured in free space and compared …
An International Comparison Of Engineering Programs In Their Emphases And Professional Skills Development, Gul Okudan Kremer, Mike Murphy, Brian Bowe
An International Comparison Of Engineering Programs In Their Emphases And Professional Skills Development, Gul Okudan Kremer, Mike Murphy, Brian Bowe
Articles
In the face of grand challenges for engineering, several efforts have been undertaken to identify the vision for what we should expect from our undergraduate engineering students. For example, one of the significant reports indicates that our graduates should aspire “to have the ingenuity of Lillian Gilbreth, the problem solving capabilities of Gordon Moore, the scientific insight of Albert Einstein, the creativity of Pablo Picasso, the determination of the Wright brothers, the leadership abilities of Bill Gates, the conscience of Eleanor Roosevelt, the vision of Martin Luther King, and the curiosity and wonder of our grandchildren.” This statement implies that …
Crystallisation And Phase Transition Characteristics Of Sol-Gel-Synthesized Zinc Titanates, Suresh Pillai, Michael Seery, Nicholas Nolan
Crystallisation And Phase Transition Characteristics Of Sol-Gel-Synthesized Zinc Titanates, Suresh Pillai, Michael Seery, Nicholas Nolan
Articles
No abstract provided.
Natural Convection In An Internally Finned Phase Change Material Heat Sink For The Thermal Management Of Photovoltaics, M. J. Huang, P. C. Eames, Brian Norton, N. J. Hewitt
Natural Convection In An Internally Finned Phase Change Material Heat Sink For The Thermal Management Of Photovoltaics, M. J. Huang, P. C. Eames, Brian Norton, N. J. Hewitt
Articles
Elevated operating temperatures reduce the solar to electrical conversion efficiency of building integrated photovoltaic devices (BIPV). Phase change materials (PCM) can be used to passively limit this temperature rise although their effectiveness is limited by their low thermal conductivities and by crystallization segregation during solidification. This paper presents an experimental evaluation of the effects of convection and crystalline segregation in a PCM as a function of efficiency of heat transfer within the finned PV/PCM system. The thermal performances of bulk PCM with crystallization segregation for different internal fin arrangements are presented. It is noted that the addition of internal fins …
Steganography Using Stochastic Diffusion For The Covert Communication Of Digital Images, Jonathan Blackledge, Abdulrahman Al-Rawi
Steganography Using Stochastic Diffusion For The Covert Communication Of Digital Images, Jonathan Blackledge, Abdulrahman Al-Rawi
Articles
This paper is devoted to the study of a method called Stochastic Diffusion for encrypting digital images and embedding the information in another host image or image set. We introduce the theoretical background to the method and the mathematical models upon which it is based. This includes a comprehensive study of the diffusion equation and its properties leading to a convolution model for encrypting data with a stochastic field that is fundamental to the approach considered. Two methods of implementing the approach are then considered. The first method introduces a lossy algorithm for hiding an image in a single host …
Eliminating Electromechanic Instablity In Dielectric Elastomers By Employing Pre-Stretch, Liang Jiang, Tony Betts, David Kennedy, Stephen Jerrams
Eliminating Electromechanic Instablity In Dielectric Elastomers By Employing Pre-Stretch, Liang Jiang, Tony Betts, David Kennedy, Stephen Jerrams
Articles
Electromechanical instability (EMI) is one of most common failure modes for dielectric elastomers (DEs). It has been reported that pre-stretching a DE sample can suppress EMI due to strain stiffening taking place for larger strains and a higher elastic modulus are achieved at high stretch ratios when a voltage is applied to the material. In this work, the influence of equi-biaxial stretch on DE secant modulus was studied using VHB 4910 and silicone rubber (SR) composites containing barium titanate (BaTiO3, BT) particles and also dopamine coated BT (DP-BT) particles. The investigation of equi-biaxial deformation and EMI failure for …
Detecting The Onset Of Dementia Using Context-Oriented Architecture, Basel Magableh, Nidal Albeiruti
Detecting The Onset Of Dementia Using Context-Oriented Architecture, Basel Magableh, Nidal Albeiruti
Articles
In the last few years, Aspect Oriented Software De- velopment (AOSD) and Context Oriented Software Development (COSD) have become interesting alternatives for the design and construction of self-adaptive software systems. An analysis of these technologies shows them all to employ the principle of the separation of concerns, Model Driven Architecture (MDA) and Component-based Software Development (CBSD) for building high quality of software systems. In general, the ultimate goal of these technologies is to be able to reduce development costs and effort, while improving the adaptability, and dependability of software systems. COSD, has emerged as a generic devel- opment paradigm towards …
Virtual Ed: Utilisation Of A Discrete Event Simulation-Based Framework In Identifying "Real-Time’ Strategies To Improve Patient Experience Times In An Emergency Department, Waleed Abo Hamad, John Mcinerney, Amr Arisha
Virtual Ed: Utilisation Of A Discrete Event Simulation-Based Framework In Identifying "Real-Time’ Strategies To Improve Patient Experience Times In An Emergency Department, Waleed Abo Hamad, John Mcinerney, Amr Arisha
Articles
Objectives and Backgrounds Emergency Department (ED) overcrowding and associated excessive Patient Experience Times (PETs) have proven deleterious impacts on patient mortality, morbidity and overall length of hospital stay. Health systems constantly seek cost-effective organisational strategies to reduce ED crowding and improve patient outcomes, but complex change implementation is constrained by the necessity of maintaining concurrent safe patient-care. Computer modelling in a “virtual reality” has been successfully utilised in industries outside medicine, in providing innovative “real-time” solutions to outdated practices. Therefore a bespoke “Virtual ED” computer model, based on a Discrete Event Simulation (DES) -Based Framework was constructed to determine the …
Application Of Stochastic Diffusion For Hiding High Fidelity Encrypted Images, Jonathan Blackledge, Abdulrahman Al-Rawi
Application Of Stochastic Diffusion For Hiding High Fidelity Encrypted Images, Jonathan Blackledge, Abdulrahman Al-Rawi
Articles
Cryptography coupled with information hiding has received increased attention in recent years and has become a major research theme because of the importance of protecting encrypted information in any Electronic Data Interchange system in a way that is both discrete and covert. One of the essential limitations in any cryptography system is that the encrypted data provides an indication on its importance which arouses suspicion and makes it vulnerable to attack. Information hiding of Steganography provides a potential solution to this issue by making the data imperceptible, the security of the hidden information being a threat only if its existence …
Non-Gaussian Analysis Of Wind Velocity Data For The Determination Of Power Quality Control, Jonathan Blackledge, Eugene Coyle, Derek Kearney
Non-Gaussian Analysis Of Wind Velocity Data For The Determination Of Power Quality Control, Jonathan Blackledge, Eugene Coyle, Derek Kearney
Articles
The quality of power (i.e. the sustainable power output as a function time) of any wind dependent energy converter (including wind turbines and wave energy converters) is determined by many design and environmental factors but timedependent variations in the wind speed are arguably the most important. In this paper we consider a non-Gaussian model for analysing and then simulating wind velocity data. In particular, we consider a Lévy distribution for the statistical characteristics of wind velocity and show how this distribution can be used to derive a stochastic fractional diffusion equation for the wind velocity as a function of time …
Using Virtual Reality To Enhance Electrical Safety And Design In The Built Environment, Martin Barrett, Jonathan Blackledge, Eugene Coyle
Using Virtual Reality To Enhance Electrical Safety And Design In The Built Environment, Martin Barrett, Jonathan Blackledge, Eugene Coyle
Articles
— Electricity and the inherent risks associated with its use in the built environment have long since been a priority for the electrical services industry and also the general public who must live and work in this environment. By its nature virtual reality has the advantage of being safe for both the user and equipment. In addition, it offers the user an opportunity to be exposed to a range of scenarios and conditions that either occur infrequently or are hazardous to replicate. This paper presents a prototype desktop virtual reality model, to enhance electrical safety and design in the built …
The Synchronized Short-Time-Fourier-Transform: Properties And Definitions For Multichannel Source Separation., Ruairí De Fréin, Scott Rickard
The Synchronized Short-Time-Fourier-Transform: Properties And Definitions For Multichannel Source Separation., Ruairí De Fréin, Scott Rickard
Articles
This paper proposes the use of a synchronized linear transform, the synchronized short-time-Fourier-transform (sSTFT), for time-frequency analysis of anechoic mixtures. We address the short comings of the commonly used time-frequency linear transform in multichannel settings, namely the classical short-time-Fourier-transform (cSTFT). We propose a series of desirable properties for the linear transform used in a multichannel source separation scenario: stationary invertibility, relative delay, relative attenuation, and finally delay invariant relative windowed-disjoint orthogonality (DIRWDO). Multisensor source separation techniques which operate in the time-frequency domain, have an inherent error unless consideration is given to the multichannel properties proposed in this paper. The sSTFT …
A Fiber Bragg Grating-Based All-Fiber Sensing Systems For Telerobotic Cutting Applications, Ginu Rajan, Dean Callaghan, Yuliya Semenova, Mark Mcgrath, Eugene Coyle, Gerald Farrell
A Fiber Bragg Grating-Based All-Fiber Sensing Systems For Telerobotic Cutting Applications, Ginu Rajan, Dean Callaghan, Yuliya Semenova, Mark Mcgrath, Eugene Coyle, Gerald Farrell
Articles
A fiber Bragg grating (FBG)-based strain sensing system for minimally invasive telerobotic cutting applications is presented in this paper. Investigations assume that a scissor blade can be approximated as a uniformly tapered cantilever beam. A replica of the scissor blade is produced and strain characterization has been carried out using an FBG sensor system. Results are validated against measurements obtained using conventional electrical resistance strain gauges. The scissor blade experiences both direct and lateral forces during cutting, hence the system is characterized for a direct load range of 0-30 N and a lateral load range of 0-10 N. The results …
The Generation Of Domestic Electricity Load Profiles Through Markov Chain Modelling, Fintan Mcloughlin, Aidan Duffy, Michael Conlon
The Generation Of Domestic Electricity Load Profiles Through Markov Chain Modelling, Fintan Mcloughlin, Aidan Duffy, Michael Conlon
Articles
Micro-generation technologies such as photovoltaics and micro-wind power are becoming increasing popular among homeowners, mainly a result of policy support mechanisms helping to improve cost competiveness as compared to traditional fossil fuel generation. National government strategies to reduce electricity demand generated from fossil fuels and to meet European Union 20/20/20 targets is driving this change. However, the real performance of these technologies in a domestic setting is not often known as high time resolution models for domestic electricity load profiles are not readily available. As a result, projections in terms of reducing electricity demand and financial paybacks for these micro-generation …
Modeling The Random Component Of Manufacturing Yield Of Integrated Circuits., David L. Farnsworth, Michael E. Long
Modeling The Random Component Of Manufacturing Yield Of Integrated Circuits., David L. Farnsworth, Michael E. Long
Articles
A model is created for the number of integrated circuits that are good from each wafer on which they are fabricated. The goal is to separate the random or common cause loss from the systematic or special loss. The random loss from this type of process is modeled so that false alarms indicating systematic loss are less likely to occur and so that the structure of the systematic loss can be determined.
Tidal Current Energy Resource Assessment In Ireland: Current Status And Future Update, Fergal O'Rourke, Fergal Boyle, Anthony Reynolds
Tidal Current Energy Resource Assessment In Ireland: Current Status And Future Update, Fergal O'Rourke, Fergal Boyle, Anthony Reynolds
Articles
Interest in renewable energy in Ireland has increased continually over the past decade. This interest is due primarily to security of supply issues and the effects of climate change. Ireland imports over 90% of its primary energy consumption, mostly in the form of fossil fuels. The exploitation of Ireland’s vast indigenous renewable energy resources is required in order to reduce this over dependence on fossil fuel imports to meet energy demand. Various targets have been set by the Irish government to incorporate renewable energy technologies into Ireland’s energy market. As a result of these targets, the development in wind energy …
A Numerical Methodology To Fully Elucidate The Altered Wall Shear Stress In A Stented Coronary Artery, Jonathan Murphy, Fergal Boyle
A Numerical Methodology To Fully Elucidate The Altered Wall Shear Stress In A Stented Coronary Artery, Jonathan Murphy, Fergal Boyle
Articles
Arterial restenosis after coronary stenting is caused by excessive tissue growth which is stimulated by arterial injury and alterations to the hemodynamic wall shear stress (WSS). Recent numerical studies have predicted only minor differences in the altered WSS between different stent designs using a commonly employed threshold assessment technique. While it is possible that there are only minor differences, it is more likely that this assessment technique is incapable of fully elucidating the alterations to the WSS created by stent implantation. This paper proposes a methodology that involves a more complete investigation into the stentinduced alterations of WSS by incorporating …
A Hybrid Highly Birefringent Fiber Optic Sensing System For Simultaneous Strain And Temperature Measurement, Piotr Lesiak, Ginu Rajan, Yuliya Semenova, Gerald Farrell, Anna Boczkowska, Andrzej Domanski, Tomasz Wolinkski
A Hybrid Highly Birefringent Fiber Optic Sensing System For Simultaneous Strain And Temperature Measurement, Piotr Lesiak, Ginu Rajan, Yuliya Semenova, Gerald Farrell, Anna Boczkowska, Andrzej Domanski, Tomasz Wolinkski
Articles
A novel hybrid fiber optic sensor based on integration of polarimetric fiber sensors for simultaneous strain and temperature measurement is presented in this paper. Two types of polarimetric fibers are used: a side-hole fiber and a polarization maintaining photonic crystal fiber. The disadvantage of each sensor type can be overcome by using the sensors in a complementary manner to measure temperature independently from average strain.
Microstrip-Fed Wideband Circularly Polarized Printed Antenna, Xiulong Bao, Max Ammann, Patrick Mcevoy
Microstrip-Fed Wideband Circularly Polarized Printed Antenna, Xiulong Bao, Max Ammann, Patrick Mcevoy
Articles
A wideband circularly-polarized printed antenna is proposed, which employs an asymmetrical dipole and a slit in the ground plane which are fed by an L-shaped microstrip feedline using a via. The proposed antenna geometry is arranged so that the orthogonal surface currents, which are generated in the dipole, feedline and ground plane, have the appropriate phase to provide circular polarization. A parametric study of the key parameters is made and the mechanism for circular polarization is described. The measured results show that the impedance bandwidth is approximately 1.34 GHz (2.45 GHz to 3.79 GHz) and the 3 dB axial ratio …
Intracellular Localisation, Geno- And Cytotoxic Response Of Poly N-Isopropylacrylamide (Pnipam) Nanoparticles To Human Keratinocyte (Hacat) And Colon Cells (Sw 480), Pratap Naha, Kunal Bhattacharya, Tiziana Tenuta, Kenneth Dawson, Iseult Lynch, Amaya Gracia, Fiona Lyng, Hugh Byrne
Intracellular Localisation, Geno- And Cytotoxic Response Of Poly N-Isopropylacrylamide (Pnipam) Nanoparticles To Human Keratinocyte (Hacat) And Colon Cells (Sw 480), Pratap Naha, Kunal Bhattacharya, Tiziana Tenuta, Kenneth Dawson, Iseult Lynch, Amaya Gracia, Fiona Lyng, Hugh Byrne
Articles
PNIPAM nanoparticles, with and without a covalentlylinked fluorescent label, were prepared by a free radical polymerisation technique. The cyto and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and zeta potential of the PNIPAM nanoparticles was carried out both in aqueous solution and in the appropriate cell culture media. Uptake and colocalisation of fluorescently labelled PNIPAM nanoparticles was monitored in both cell lines using confocal laser scanning microscopy. Genotoxicity analysis using the Comet assay was performed in both cell lines …