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Full-Text Articles in Engineering

Fate And Transport Of Antibiotics And Antibiotic Resistance Genes In Runoff And Soil As Affected By The Timing Of Swine Manure Slurry Application, Renys Enrique Barrios, Himanshu Khuntia, Shannon L. Bartelt-Hunt, John E. Gilley, Amy M. Schmidt Schmidt, Daniel D. Snow, Xu Li Apr 2020

Fate And Transport Of Antibiotics And Antibiotic Resistance Genes In Runoff And Soil As Affected By The Timing Of Swine Manure Slurry Application, Renys Enrique Barrios, Himanshu Khuntia, Shannon L. Bartelt-Hunt, John E. Gilley, Amy M. Schmidt Schmidt, Daniel D. Snow, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Land application of swine manure slurry is a common practice to supplement nutrients to soil for crop production. This practice can introduce antibiotic residues and antibiotic resistance genes (ARGs) into the environment. Field testing is critical in identifying manure management practices effective in minimizing the environmental impacts of manure-borne antibiotic and ARGs. The objective of this study was to determine how the timing of swine manure application relative to rainfall events impacts the fate and transport of antibiotics and ARGs in surface runoff and manure-amended soil. Swine manure slurry was either broadcast or injected on test plots in the field. …


Occurrence And Persistence Of Antibiotics Administered To Cattle In A Newly Established Feedlot, Brittany Trejo Apr 2020

Occurrence And Persistence Of Antibiotics Administered To Cattle In A Newly Established Feedlot, Brittany Trejo

Department of Environmental Engineering: Theses and Student Research

The common practice of using therapeutic antibiotics in livestock farming is a worldwide phenomenon. Over the last decade, there has been a growing concern of antibiotics entering the environment via animal manure. Similar studies have focused on the occurrence and biological effects of antibiotics in land-applied animal feedlots; however, limited research has been conducted on the occurrence and persistence of antibiotics in animal feedlots. A study was conducted to investigate the occurrence and persistence of four injected antibiotics (ceftiofur enrofloxacin, florfenicol, and tulathromycin) and two continuously fed antibiotics (monensin and tylosin) in feedlot sediment, runoff, and sediment runoff. For antibiotics …


Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants, Jon Duerschner, Shannon L. Bartelt-Hunt, Kent Eskridge, John E. Gilley, Xu Li, Amy M. Schmidt, Daniel D. Snow Jan 2020

Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants, Jon Duerschner, Shannon L. Bartelt-Hunt, Kent Eskridge, John E. Gilley, Xu Li, Amy M. Schmidt, Daniel D. Snow

Nebraska Water Center: Faculty Publications

Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. …


Measuring The Occurrence Of Antibiotics In Surface Water Adjacent To Cattle Grazing Areas Using Passive Samplers, Nasrin Naderi Beni, Daniel D. Snow, Elaine D. Berry, Aaron R. Mittelstet, Tiffany Messer, Shannon L. Bartelt-Hunt Jan 2020

Measuring The Occurrence Of Antibiotics In Surface Water Adjacent To Cattle Grazing Areas Using Passive Samplers, Nasrin Naderi Beni, Daniel D. Snow, Elaine D. Berry, Aaron R. Mittelstet, Tiffany Messer, Shannon L. Bartelt-Hunt

Nebraska Water Center: Faculty Publications

A wide variety of antibiotics and other pharmaceuticals are used in livestock production systems and residues passed to the environment, often unmetabolized, after use and excretion. Antibiotic residuesmay be transported frommanure-treated soils via runoff and are also capable of reaching surface and groundwater systems through a variety of pathways. The occurrence and persistence of antibiotics in the environment is a concern due to the potential for ecological effects and proliferation of environmental antibiotic resistance in pathogenic organisms. In the present study, the occurrence and seasonal variation of 24 commonly-used veterinary antibiotics was evaluated in surface water adjacent to several livestock …


High-Throughput Screening Of Clinically Approved Drugs That Prime Nonviral Gene Delivery To Human Mesenchymal Stem Cells, Tyler Kozisek, Andrew Hamann, Albert Nguyen, Michael Miller, Sarah Plautz, Angela K. Pannier Jan 2020

High-Throughput Screening Of Clinically Approved Drugs That Prime Nonviral Gene Delivery To Human Mesenchymal Stem Cells, Tyler Kozisek, Andrew Hamann, Albert Nguyen, Michael Miller, Sarah Plautz, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Background: Human mesenchymal stem cells (hMSCs) are intensely researched for applications in cell therapeutics due to their unique properties, however, intrinsic therapeutic properties of hMSCs could be enhanced by genetic modification. Viral transduction is efficient, but suffers from safety issues. Conversely, nonviral gene delivery, while safer compared to viral, suffers from inefficiency and cytotoxicity, especially in hMSCs. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological ‘priming’ of hMSCs with the glucocorticoid dexamethasone can significantly increase transfection in hMSCs by modulating transfection-induced cytotoxicity. This work seeks to establish a library of transfection …


Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants, Jon Duerschner, Shannon L. Bartelt-Hunt, Kent Eskridge, John E. Gilley, Xu Li, Amy M. Schmidt, Daniel D. Snow Jan 2020

Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants, Jon Duerschner, Shannon L. Bartelt-Hunt, Kent Eskridge, John E. Gilley, Xu Li, Amy M. Schmidt, Daniel D. Snow

Department of Biological Systems Engineering: Papers and Publications

Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. …


Influence Of Setback Distance On Antibiotics And Antibiotic Resistance Genes In Runoff And Soil Following The Land Application Of Swine Manure Slurry, Maria C. Hall, Noelle Mware, John E. Gilley, Shannon L. Bartelt-Hunt, Daniel D. Snow, Amy M. Schmidt Schmidt, Kent M. Eskridge, Xu Li Jan 2020

Influence Of Setback Distance On Antibiotics And Antibiotic Resistance Genes In Runoff And Soil Following The Land Application Of Swine Manure Slurry, Maria C. Hall, Noelle Mware, John E. Gilley, Shannon L. Bartelt-Hunt, Daniel D. Snow, Amy M. Schmidt Schmidt, Kent M. Eskridge, Xu Li

Department of Biological Systems Engineering: Papers and Publications

The environmental spread of antibiotics and antibiotic resistance genes (ARGs) from the land application of livestock wastes can be a potential public health threat. The objective of this study was to assess the effects of setback distance, which determines how close manure may be applied in relation to surface water, on the transport of antibiotics and ARGs in runoff and soil following land application of swine manure slurry. Rainfall simulation tests were conducted on field plots covered with wheat residues, each of which contained an upslope manure region where slurry was applied and an adjacent downslope setback region that did …


Environmental Fate And Microbial Effects Of Monensin, Lincomycin, And Sulfamethazine Residues In Soil, Matteo D'Alessio, Lisa M. Durso, Daniel N. Miller, Brian Woodbury, Chittaranjan Ray, Daniel D. Snow Nov 2018

Environmental Fate And Microbial Effects Of Monensin, Lincomycin, And Sulfamethazine Residues In Soil, Matteo D'Alessio, Lisa M. Durso, Daniel N. Miller, Brian Woodbury, Chittaranjan Ray, Daniel D. Snow

Nebraska Water Center: Faculty Publications

The impact of commonly-used livestock antibiotics on soil nitrogen transformations under varying redox conditions is largely unknown. Soil column incubations were conducted using three livestock antibiotics (monensin, lincomycin and sulfamethazine) to better understand the fate of the antibiotics, their effect on nitrogen transformation, and their impact on soil microbial communities under aerobic, anoxic, and denitrifying conditions. While monensin was not recovered in the effluent, lincomycin and sulfamethazine concentrations decreased slightly during transport through the columns. Sorption, and to a limited extent degradation, are likely to be the primary processes leading to antibiotic attenuation during leaching. Antibiotics also affected microbial respiration …


Effect Of Swine Manure Pit Additives And Facility Disinfectants On The Fate Of Antibiotics And Manure Composition During Simulated Swine Manure Slurry Storage, Jon Duerschner Nov 2018

Effect Of Swine Manure Pit Additives And Facility Disinfectants On The Fate Of Antibiotics And Manure Composition During Simulated Swine Manure Slurry Storage, Jon Duerschner

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

This thesis investigates the effect of time and of swine manure slurry treatment on the physical properties, nutrient content, and the concentrations of antibiotics chlortetracycline, lincomycin, and tiamulin in simulated storage of swine manure. In one experiment the slurry was treated with six additive products. In a second experiment a set of four disinfectant products were used. Control consisted of unamended slurry. Manure was stored in 60 liter stainless steel bioreactors to simulate deep pit storage and was sampled 7 times over a 40 day incubation.

From an ANOVA of the results, it was concluded that evaporation may be contributing …


Detection, Occurrence And Fate Of Emerging Contaminants In Agricultural Environments, Daniel D. Snow, David A. Cassada, Saptashati Biswas, Mohammedreza Shafieifini, Xu Li, Matteo D'Alessio, Laura Carter, J. Brett Sallach Oct 2018

Detection, Occurrence And Fate Of Emerging Contaminants In Agricultural Environments, Daniel D. Snow, David A. Cassada, Saptashati Biswas, Mohammedreza Shafieifini, Xu Li, Matteo D'Alessio, Laura Carter, J. Brett Sallach

Daugherty Water for Food Global Institute: Faculty Publications

A total of 75 papers published in 2017 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, and engineered nanoparticles agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Fate and Occurrence, Pharmaceutical Metabolites, Anthelmintics, Antibiotic Resistance Genes, and Engineered Nanomaterials.