Engineering Commons^™

Irt-Based Irrigation Scheduling And Dry Bean Crop Water Productivity Under Different Irrigation Scenarios In The Nebraska High Plains, Angie Gradiz

Department of Biological Systems Engineering: Dissertations and Theses

Irrigation plays a critical role for crop production in western Nebraska of the United States, where crop evapotranspiration often exceeds in-season precipitation. This study investigated the impacts of different irrigation treatments on yield and key physiological parameters of dry edible beans grown in semi-arid western Nebraska during the 2022-2023 growing seasons. Seven irrigation levels (0%, 25%, 50%, 75%, 100%, 125%, 150%) of the fully irrigated treatment (FIT), were applied using a Variable Rate Irrigation (VRI) Linear-Move Sprinkler System. Additionally, an eighth treatment was scheduled based on measurement from infrared radiometry thermometers (IRT) to evaluate the performance of the two-source energy …

Soil Nitrate Prediction Using Ftir-Atr, Vis-Nir, And Raman Spectroscopy, Sabiha Ferdous

Department of Biological Systems Engineering: Dissertations and Theses

Fourier transform infrared spectroscopy (FTIR) combined with Attenuated total reflectance (ATR), Visible-Near Infrared spectroscopy (Vis-NIR), and Raman spectroscopy (RS) are non-destructive techniques for rapid determination of nitrogen compounds in soil. Leveraging FTIR-ATR and Vis-NIR spectra using partial least squares regression (PLSR) modeling, the study aims to predict soil nitrate content and explored the feasibility of Raman spectroscopy to detect nitrate (NO₃^-), nitrite (NO₂^-), and ammonium (NH₄⁺) in soil. Soil samples were collected from four different fields, dried, sieved (2mm), and then used for collecting spectra (FTIR-ATR and Vis-NIR). Laboratory analysis was …

Development Of A Machine Learning System For Irrigation Decision Support With Disparate Data Streams, Eric Wilkening

Department of Biological Systems Engineering: Dissertations and Theses

In recent years, advancements in irrigation technologies have led to increased efficiency in irrigation applications, encompassing the adoption of practices that utilize data-driven irrigation scheduling and leveraging variable rate irrigation (VRI). These technological improvements have the potential to reduce water withdrawals and diversions from both groundwater and surface water sources. However, it is vital to recognize that improved application efficiency does not necessarily equate to increased water availability for future or downstream use. This is particularly crucial in the context of consumptive water use, which refers to water consumed and not returned to the local or sub-regional watershed, representing a …

Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee

Department of Biological Systems Engineering: Dissertations and Theses

With a growing human population, urbanization is impeding a plethora of natural waterways. Of these, urban ponds play a vital role in nutrient sequestration, flood prevention, and habitat sanctuaries. However, nutrient loading can reduce habitat effectiveness and promote harmful algae blooms. To reduce internal nutrient loads, a biological-chemical treatment strategy consisting of floating treatment wetlands (FTWs) and lanthanum were applied to two urban retention ponds, Densmore and Wilderness Ridge Ponds. To measure effectiveness, chlorophyll-a samples were collected and correlated with Sentinel-2. A novel band algorithm termed 3BR1 produced a strong correlation (R² = 0.72) to physical chlorophyll-a …

Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto

Department of Biological Systems Engineering: Dissertations and Theses

The emissions of ammonia (NH₃), particulate matter (PM_2.5), and carbon dioxide (CO₂) are major concerns in poultry facilities. They can pose environmental concerns and nuances. Robust and affordable measurement systems are needed to accurately measure in-barn concentrations and quantify the emissions.

The Intelligent Portable Monitoring Unit (iPMU or PMU3) developed in 2016 was reconstructed into PMU4 to include upgraded NH₃ and PM_2.5 sensors and wireless connectivity for a low-cost, robust, and accurate air quality monitoring device with contactless data transfer using the concept of Internet of Things (IoT). In addition, a user-friendly …

Responses Of Maize To Different Irrigation Regimes In Semi-Arid Western Nebraska, Swathi Palle

Department of Biological Systems Engineering: Dissertations and Theses

As the “Cornhusker State”, Maize (Zea mays) is an important crop in Nebraska. However, farmers in the state are challenged by unstable supply of surface water and limited groundwater resources. A better understanding of maize's response to water stress that occurs at different growth stages can help implement the best irrigation practices that conserve water while maintaining yields. In this study, we conducted field experiments at the Panhandle Research, Extension, and Education Center to compare the responses of maize to eight irrigation treatments which included both limited and deficit irrigation regimes during 2022 and 2023 growing seasons. Specifically, …

Modeling Overdraft-Driven Nitrate Transport In Shallow Wells For Mitigation And Scenario Planning, Jonathan Cronk

Department of Biological Systems Engineering: Dissertations and Theses

In Nebraska, average nitrate concentrations in groundwater have doubled since 1974, making water quality management more important than ever. As droughts, heat waves, and floods become more common climate events, understanding their impacts will be necessary to make informed management decisions. Emerging literature describes that drought correlates to an increase in the concentration of nitrate-N at domestic and irrigation wells, however the relative contributions of the mechanisms thought to be responsible is currently unknown.

This research assessed the impact of recharge and pumping rate changes as two mechanisms affecting nitrate-N concentration during drought, assessed the relationship between well depth and …

Plant Height Estimation Using Rtk-Gnss Enabled Unmanned Aerial Vehicle (Uav) Photogrammetry, Pascal Izere

Department of Biological Systems Engineering: Dissertations and Theses

Using Unmanned Aerial Vehicle (UAV) based remote sensing technology to provide high-throughput plant phenotypic traits has shown a great potential of eliminating manual measurements. For example, the use of UAV imaging to estimate plant height is of great interest to plant breeders and agronomists. Height is linked to a plant’s physical growth, light competition, health, biomass, and yield; thus, its accurate estimation is crucial in breeding. Conventionally, accurate height estimation by UAV platforms relies heavily on the use of ground control points (GCPs) for geometric calibration due to the relatively low positioning accuracy of regular differential global navigation satellite system …

Characterizing Feedlot Feed Using Depth Cameras And Imaging Technology, Jean Niwenshuti

Department of Biological Systems Engineering: Dissertations and Theses

Imaging technology is a growing field that provides solutions in many areas from manufacturing to agriculture. Through previous research, imaging technologies have been studied in livestock farming to monitor the animal’s health and welfare in the production process. However, the feedlot industry is still behind in validating the feasibility to use some of these technologies nor to adopt such technologies to address challenges the industry is facing, such as lack of skilled labor.This work proposes using novel imaging methods to identify feed types and estimate the amount of feed remaining in a typical Midwestern feedlot feed bunk. These methods have …

Assessment Of Integrated Nutrient Management Practices On Soil Health And Nitrogen Efficiency In Cropping Systems, María José Oviedo Ventura

Department of Biological Systems Engineering: Dissertations and Theses

Groundwater quality in Nebraska is threatened by non-point source contributions of nitrate-nitrogen, primarily by agricultural systems. Intensive crop and livestock production that neglects the value of manure in crop fertility plans can contribute to soil degradation and natural resource impairment. The purpose of this study is to evaluate opportunities to improve agricultural nutrient management throughout Nebraska by quantifying opportunities for manure distribution, evaluating soil health implications of organic and inorganic soil amendments, and assessing nutrient management practices focused on reducing nitrate leaching in agricultural soils.

This study described in Chapter 2 provides a spatial illustration of nitrogen and phosphorus balances …

A Robotic System For In-Situ Measurement Of Soil Total Carbon And Nitrogen, Husein Harun

Department of Biological Systems Engineering: Dissertations and Theses

Surges in the cost of fertilizer in recent times coupled with the environmental effects of their over-application have driven the need for farmers to optimize the amount of fertilizer they apply on the farm. One of the key steps in determining the right amount of fertilizer to apply in a given field is measuring the amount of nutrients present in the soil. To ascertain nutrient deficiencies, most farmers perform wet chemistry analysis of soil samples which requires a lot of time and is expensive. In this research project, a robotic system was designed and developed that could autonomously move to …

Engineering Of Microparticle Encapsulated Antioxidant To Mitigate Oxidative Stress In Vitro And In Vivo, Kayla Ney

Department of Biological Systems Engineering: Dissertations and Theses

Chronic low back pain (cLBP) is one of the leading causes of years lived with disability in the United States. Current treatments for cLBP have variable results across the patient population, and many patients struggle to find consistent relief. Most current treatments focus on the symptom of pain, not the root cause. In painful degenerated discs, oxidative stress and inflammation function in a vicious cycle and perpetuate degeneration, damage, and pain. Therefore, oxidative stress and inflammation are important targets in addressing the source of pain. This work characterizes an antioxidant, BuOE, encapsulated in a chondroitin sulfate microparticle as a novel …

Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa

Department of Biological Systems Engineering: Dissertations and Theses

Plant phenotyping has been recognized as a rapidly growing field of research due to the labor-intensive, destructive, and time-consuming nature of traditional phenotyping methods. These phenotyping bottlenecks can be addressed by advancements in image-based phenotyping like RGB and hyperspectral imaging for the assessment of plant traits important for breeding purposes. This study aims (1) to characterize the physical and biochemical traits of wheat and corn plants using RGB and hyperspectral imaging in the greenhouse, and (2) to estimate leaf nitrogen (N), phosphorus (P), and potassium (K) content using hyperspectral imaging and an analytical spectral device (ASD spectrometer) and compare the …

Estimating Crop Stomatal Conductance Through High-Throughput Plant Phenotyping, Junxiao Zhang

Department of Biological Systems Engineering: Dissertations and Theses

During photosynthesis and transpiration, crops exchange carbon dioxide and water with the atmosphere through stomata. When a crop experiences water stress, stomata are closed to reducing water loss. However, the closing of stomata also negatively affects the photosynthetic efficiency of the crop and leads to lower yields. Stomatal conductance (gs) quantifies the degree of stomatal opening and closing by using the rate of gas exchange between the crop and the atmosphere, which helps to understand the water status of the crop for better irrigation management. Unfortunately, gs measurement typically requires contact measuring instruments and manual collection in the field, which …

Spectroscopic Sensor Data Fusion To Improve The Prediction Of Soil Nutrient Contents, Bidhan Ghimire

Department of Biological Systems Engineering: Dissertations and Theses

This study aims to advance the understanding and application of spectroscopic sensor data fusion for improving soil nutrient content predictions. In addition to presenting an extensive review of studies on the spectroscopic sensor data fusion, a research investigation was conducted to assess the effectiveness of five fusion algorithms in predicting three primary nutrients (Nitrogen, Phosphorous, and Potassium) and two secondary nutrients (Calcium and Magnesium) in soil using Visible and Near-Infrared, Mid-Infrared, and X-ray Fluorescence data. Among the five fusion algorithms, one was a low-level fusion involving data concatenation. Two were mid-level fusions, incorporating feature extraction by applying (i) Principal Component …

Agen/Bsen 112 Final Project: Moving And Temperament Of Cattle, Elly Vo, Taetum Baxa, Ved Patel, Brady Giles, Tami M. Brown-Brandl, Rossana Villa-Rojas

Department of Biological Systems Engineering: Dissertations and Theses

Cattle movement and weather may affect the body temperature of cows which in turn affects their natural behavior and can influence their metabolism. Cattle take several days to resume their normal eating patterns after being overheated, and that can affect dairy production. This project was assigned to study the effect of temperament (calm vs excitable) and evaporative cooling on the body temperature of moving animals to optimize the environmental conditions around the cattle and consequently, dairy production.

The project began with processing of a data set from Dr. Tami Brown-Brandl who was the client/adviser for the group. The data contained …

Metabolic Network Modeling To Unravel The Impact Of Copper Deficiency And High-Fat Diet On Liver Metabolism, Naeun Lee

Department of Biological Systems Engineering: Dissertations and Theses

Fatty liver disease is a common health problem caused by an imbalance in the intake, utilization, and distribution of energy. High-fat and high-carbohydrate diets have been identified as major risk factors for fatty liver disease. Other contributors and modifiers can also influence the development of liver damage. Intriguingly, inappropriate copper (Cu) levels in the diet and organs are associated with fatty liver disease, though the exact way in which Cu deficiency or toxicity contributes to metabolic issues is not yet clear. Cu is necessary for several biological processes, including energy production and redox balance. Further research should be taken to …

Semantic Segmentation Based Deep Learning Approaches For Weed Detection, Puranjit Singh

Department of Biological Systems Engineering: Dissertations and Theses

Global increase in herbicide use to control weeds has led to issues such as evolution of herbicide-resistant weeds, off-target herbicide movement, etc. Precision agriculture advocates Site Specific Weed Management (SSWM) application to achieve precise and right amount of herbicide spray and reduce off-target herbicide movement. Recent advancements in Deep Learning (DL) have opened possibilities for adaptive and accurate weed recognitions for field based SSWM applications with traditional and emerging spraying equipment; however, challenges exist in identifying the DL model structure and train the model appropriately for accurate and rapid model applications over varying crop/weed growth stages and environment. In our …

Production Of Polyhydroxybutyrate From Corn Fiber Using Paraburkholderia Sacchari, Twissa Mitra

Department of Biological Systems Engineering: Dissertations and Theses

Poly(3-hydroxybutyrate) (PHB) is one of the most broadly studied members of the polyhydroxyalkanoates (PHA) family. It is a fully biodegradable thermoplastic produced by microbial fermentation having properties similar to polypropylene. PHB has a market price that is three times higher than the cost of synthetic plastics. Substrate cost makes up 50 to 60 % of that cost; hence, researchers have been exploring various cheap natural resources with high carbohydrate content to be used as a substrate for PHB production.

In this study, corn fiber, a lignocellulosic biomass has been studied as an option for a substrate to produce PHB. This …

Cybersecurity Of Agricultural Machinery: Exploring Cybersecurity Risks And Solutions For Secure Agricultural Machines, Mark Freyhof

Department of Biological Systems Engineering: Dissertations and Theses

Modern agriculture is reliant on agricultural machinery for the production of food, fuel, and other agricultural products. The need for producing large quantities of quality agricultural products while sustainably stewarding environmental resources has led to the integration of numerous digital technologies into modern agricultural machinery, such as the CAN bus and telematic control units (Liu et al., 2021). An unintended drawback of these integrated digital technologies is the opportunity for these components to become cyberattack vectors. Cyberattack instances have increasingly targeted critical infrastructures, with numerous reports from agencies such as the Federal Bureau of Investigation (FBI) and Department of Homeland …

Pesticide Application Management Toolset For Worker Protection Standards, Nathan W. Thorson

Department of Biological Systems Engineering: Dissertations and Theses

The application of Pesticides has become a widely adopted practice within modern agriculture, however this practice poses a significant health risk to farm workers and crop advisors when pesticide exposures occur. Current safety standards require documentation of each application to help mitigate human exposure, yet these standards rely heavily on antiquated data collection formats and complex communication systems putting little emphasis on notification timeliness. Our objective was to reduce the risk of pesticide exposure for all farm workers and crop advisors by utilizing technological automation processes to reduce the number of links within the communication system. This proved successful though …

Development Of A Decellularized Hydrogel Composite And Its Application In A Novel Model Of Disc-Associated Low Back Pain In Female Sprague Dawley Rats, David Lillyman

Department of Biological Systems Engineering: Dissertations and Theses

Chronic low back pain is a global socioeconomic crisis compounded by an absence of reliable, curative treatments. The predominant pathology associated with chronic low back pain is degeneration of intervertebral discs in the lumbar spine. During degeneration, nerves can sprout into the intervertebral disc tissue and be chronically subjected to inflammatory and mechanical stimuli, resulting in pain. Pain arising from the intervertebral disc, or disc-associated pain, is a complex, multi-faceted disorder which necessitates valid animal models to screen therapeutics and study pathomechanisms of pain.

While many research teams have created animal models of disc degeneration, the translation of these platforms …

Studies On Air Injection In A Wastewater-Fed Subsurface Drip Irrigation System, Henry A. Gonzalez Hernandez

Department of Biological Systems Engineering: Dissertations and Theses

In 2020, the Ogallala High Plains Aquifer supplied irrigation water for approximately 3.6 million hectares in Nebraska, making it the most irrigated state in the United States. Although Nebraska is highly dependent in groundwater, locations included in the Gering-Fort Laramie Irrigation District (border of Nebraska and Wyoming) rely on surface water resources. In addition to these conventional water sources, using non-conventional alternatives for crop production can be just as important during water shortage times such as the one that e occurred in 2019 with the collapse of the Goshen/Gering-Fort Laramie Canal. Feedlot runoff presents a great opportunity in Nebraska, but …

Understanding The Role Of Antioxidant Nanoparticles In Improving The Outcome Of Secondary Injury In Traumatic Brain Injury, Aria W. Tarudji

Department of Biological Systems Engineering: Dissertations and Theses

Following traumatic brain injury (TBI), excess reactive oxygen species (ROS) and other free radicals are released, inducing the cascade of secondary injury that exacerbate the outcomes of TBI. Antioxidant nanoparticles (ANPs) have shown promising outcomes in reducing the progression of TBI, which may be due to the higher accumulation and retention of ANPs in the injured brain. However, there is limited knowledge of: 1) antioxidant activities needed in TBI treatment, 2) correlation between longer retention, bioavailability, and target engagement with antioxidant treatments, and 3) sexual dimorphism to ANP treatments.

This dissertation assesses multiple ANPs with various scavenging activities and durations …

Effect Of Injury Mechanism And Severity On The Molecular Pathophysiology Of Traumatic Brain Injury, Brandon Mcdonald

Department of Biological Systems Engineering: Dissertations and Theses

Traumatic brain injury (TBI) mechanism and severity are heterogenous clinically, resulting in a multitude of physical, cognitive, and behavioral deficits. However, approximately 80% suffer from milder injuries. Thus, examining pathophysiological changes associated with mild TBI is imperative for improving clinical translation and evaluating the efficacy of potential therapeutic strategies. Through this work, we developed models of TBI, ranging in both injury mechanism and severity, using an electromagnetic controlled cortical impact (CCI) device. First, we characterized and optimized a closed head, mild TBI model (DTBI) to determine the clinical translatability and practicality of producing repeated mild injuries. Interestingly, we determined that …

Optimization Of A Novel Barnes Maze Protocol For Assessing Antioxidant Treatment Of Traumatic Brain Injury, Connor C. Gee

Department of Biological Systems Engineering: Dissertations and Theses

Current preclinical research into traumatic brain injury focuses heavily upon cellular and molecular testing to determine the effects of injury and potential benefits of neuroprotective treatments. While this may be a useful method, some argue that an increased focus on behavioral testing could lead to better clinical translation as these assays assess the longer term, downstream effects from a brain injury. The most characterized behavioral tests used in traumatic brain injury research are the spatial learning and memory paradigms, Morris Water Maze and Barnes Maze. The Morris Water Maze is the most used of theses paradigms and relies on spatial …

Inactivation Of Salmonella In Black Peppercorn By Fluidization With Hydrogen Peroxide Vapor, Edel Summers

Department of Biological Systems Engineering: Dissertations and Theses

The purpose of the present study was to assess the effectiveness of hydrogen peroxide vapor (HPV) at inactivating Salmonella inoculated in whole black pepper when used as fluidizing gas media. HPV treatment was conducted at two different temperatures (45°C and 60°C) and two dwell times (30 min and 60 min). Microbial reduction and residual hydrogen peroxide were measured at three storage times: 0 h (immediately after treatment), 24 h and 48 h post-treatment. The effect of HPV on the quality of whole black peppercorn was evaluated 48 h post-treatment based on changes in piperine content, total phenolics, antioxidant activity, total …

Assessment Of Manure Treatment Technology Adoption And Feedlot Runoff Monitoring Opportunities, Juan Carlos Ramos Tanchez

Department of Biological Systems Engineering: Dissertations and Theses

Livestock products contain valuable nutrients that enhance human health, and their production generates positive local and national economic impacts. The livestock sector also deals with large quantities of environmentally important nutrients in manure. The purpose of this thesis is to further the aims of researchers and educators working at the intersection of livestock production and the environment to identify and promote best practices for livestock production and manure management that are economically and environmentally sustainable.

The second chapter explored options to evaluate nutrient flows in feedlot runoff at the pen scale. Pen scale monitoring is an important consideration for research …

Development And Characterization Of A Decellularized Neuroinhibitory Scaffold Containing Matrix Bound Nanovesicles, Logan Piening

Department of Biological Systems Engineering: Dissertations and Theses

Chronic low back pain (LBP) is a leading cause of disability but treatments for LBP are limited. Degeneration of the intervertebral disc leads to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) which allows nerves from dorsal root ganglia (DRG) to grow into the core of the disc, leading to pain. Current treatments for LBP involve drugs that do not target the source of the pain and lack long term efficacy or use invasive surgeries with high complication rates. Treatment with a decellularized tissue scaffold that contains neuroinhibitory components may inhibit nerve growth and prevent disc-associated LBP. Here, a decellularized nucleus pulposus …

Evaluation Of A Novel Approach For Assessing Biological Activity In Agricultural Soils, Karla Melgar

Department of Biological Systems Engineering: Dissertations and Theses

Soil health is a key factor impacting soil resilience and fertility in crop production systems. Favorable soil physical and biological conditions facilitate plant nutrient absorption and nutrient cycling. Demonstrating to farmers the impacts and changes in soil biological activity under different soil management practices has been a challenge due to the limited availability of inexpensive tools for quantifying this component of soil health. The primary goal of this study was to present a simple and readily accessible tool for evaluating soil biological activity to promote the use of organic amendments in crop fields. Research plots were established in two studies …