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Articles 31 - 32 of 32
Full-Text Articles in Engineering
Development And Application Of Screening Scale Bioreactor Systems For Very High Cell Density Perfusion Of Mammalian Cells, Caijuan Zhan, Hubert Schwarz, Magnus Lundqvist, Atefeh Shokri, Ray Field, Richard Turner, Mathias Uhlén, Johan Rockberg, Veronique Chotteau
Development And Application Of Screening Scale Bioreactor Systems For Very High Cell Density Perfusion Of Mammalian Cells, Caijuan Zhan, Hubert Schwarz, Magnus Lundqvist, Atefeh Shokri, Ray Field, Richard Turner, Mathias Uhlén, Johan Rockberg, Veronique Chotteau
Integrated Continuous Biomanufacturing III
The development of cultivation processes can be significantly more efficient and cost effective when performed in parallel screening systems operated at small scale. Although perfusion systems at screening scale have been proposed, none of them enables a real mimic of very high cell density perfusion bioreactors. The latter is however important to maximize the performance of processes at 80, 100 x 106 cells/mL, or higher cell densities, in particular to optimize the nutrient supply and feeding regime. The purpose of the present work was to develop such a system and to apply it to perfusion optimization. Chinese Hamster Ovary cells …
Process Considerations For Protein A Affinity Capture, Virus Inactivation, And Linked Polishing Steps In Multi-Column Continuous Purification Of Monoclonal Antibodies, Robert Mierendorf, Tom Van Oosbree, Anthony Grabski, William Wessel, Emily Schirmer
Process Considerations For Protein A Affinity Capture, Virus Inactivation, And Linked Polishing Steps In Multi-Column Continuous Purification Of Monoclonal Antibodies, Robert Mierendorf, Tom Van Oosbree, Anthony Grabski, William Wessel, Emily Schirmer
Integrated Continuous Biomanufacturing III
Multi-column continuous chromatography (MCC) offers significant economic advantages over traditional batch methods for purification of monoclonal antibodies (mAbs), including increased resin capacity utilization, smaller columns, reduced buffer consumption, and faster process time. The Protein A capture step is a primary target to apply MCC due to its high cost, which is driven even higher as improvements in upstream processing have produced a steady increase in mAb titers. In this study we consider the key factors in designing an MCC process that optimizes productivity. Process design begins with determination of the feed duration, which is governed by the mAb titer, column …