Engineering Commons^™

3d Interdigitated Vertically Aligned Carbon Nanotube Electrodes For Electrochemical Impedimetric Biosensing, Benjamin J. Brownlee, Jonathan C. Claussen, Brian D. Iverson

Faculty Publications

Advances in nanomaterials, combined with electrochemical impedance spectroscopy (EIS), have allowed electrochemical biosensors to have high sensitivity while remaining labe-lfree, enabling the potential for portable diagnosis at the point-of-care. We report porous, 3D vertically aligned carbon nanotube (VACNT) electrodes with underlying chromium electrical leads for impedance-based biosensing. The electrodes are characterized by electrode height (5, 25, and 80 μm), gap width (15 and 25 μm), and geometry (interdigitated and serpentine) using scanning electron microscopy, cyclic voltammetry, and EIS. The protein streptavidin is functionalized onto VACNT electrodes for detection of biotin, as confirmed by fluorescence microscopy. EIS is used to measure …