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Articles 6631 - 6660 of 40978
Full-Text Articles in Engineering
Moving Lab-Based In-Person Training To Online Delivery: The Case Of A Continuing Engineering Education Program, Catherine Maware, David M. Parsley Ii, Kun Huang, Gerry M. Swan, Nelson Akafuah
Moving Lab-Based In-Person Training To Online Delivery: The Case Of A Continuing Engineering Education Program, Catherine Maware, David M. Parsley Ii, Kun Huang, Gerry M. Swan, Nelson Akafuah
Institute of Research for Technology Development Faculty Publications
Background: Online learning has recently surged due to the COVID-19 global pandemic. Because of the pandemic, many universities were forced to move to online learning, and various online teaching and learning environments emerged, both asynchronous and synchronous.
Objective: This study explores how a large university in the Southeastern United States of America converted an in-person Lean Manufacturing professional course into synchronous online learning for industry participants.
Method: The study analysed the performance of 212 in-person and 43 online industry participants and examined the views of online participants about the training. Paired t-tests, one-way multivariate analysis of covariance (MANCOVA), and univariate …
A Robust Model Reduction For The Optimal Boundary Feedback Stabilization Of Magnetizable Piezoelectric Beams, Md Rafi As Sadeq Ibn Emran
A Robust Model Reduction For The Optimal Boundary Feedback Stabilization Of Magnetizable Piezoelectric Beams, Md Rafi As Sadeq Ibn Emran
Masters Theses & Specialist Projects
The one-dimensional Partial Differential Equation (PDE) model of the wave equation, which describes wave dynamics of a wide-range of controlled mechanical systems, with a state feedback controller at its boundary is known to have exponentially stable solutions. Moreover, it is also reported that the several model reductions of the wave equation by the standard Finite Differences and Finite Elements approximations suffer from the lack of exponential stability (and exact observability without a state feedback controller) uniformly as the discretization parameter tends to zero. This is due to the loss of a uniform gap among the high-frequency eigenvalues as the discretization …
Controllable Language Generation Using Deep Learning, Rohola Zandie
Controllable Language Generation Using Deep Learning, Rohola Zandie
Electronic Theses and Dissertations
The advent of deep neural networks has sparked a revolution in Artificial Intelligence (AI), notably with the creation of Transformer models like GPT-X and ChatGPT. These models have surpassed previous methods in various Natural Language Processing (NLP) tasks. As the NLP field evolves, there is a need to further understand and question the capabilities of these models. Text generation, a crucial part of NLP, remains an area where our comprehension is limited while being critical in research.
This dissertation focuses on the challenging problem of controlling the general behaviors of language models such as sentiment, topical focus, and logical reasoning. …
Hydrological Dynamics Of Surface-Groundwater Interactions Between Major Springs Of Mammoth Cave And The Green River, Kentucky, Usa, Matthew Cecil
Hydrological Dynamics Of Surface-Groundwater Interactions Between Major Springs Of Mammoth Cave And The Green River, Kentucky, Usa, Matthew Cecil
Masters Theses & Specialist Projects
Mammoth Cave is an iconic example of the cave and karst systems that underlie over 50% of the United States and provide around 13% of the world’s population with water resources. These environmental features are vulnerable to drought, pollution, and human impacts. The complex hydrological regime of the Mammoth Cave system has been studied in the past, but there are improvements and updates that can be made to the past research. The dynamics of the surface-groundwater system, particularly reversals of the Green River and how it backfloods into the cave, are some examples. Echo River and River Styx Springs provide …
Activation Of The Renin–Angiotensin–Aldosterone System Is Attenuated In Hypertensive Compared With Normotensive Pregnancy, Robin C. Shoemaker, Marko Poglitsch, Hong Huang, Katherine Vignes, Aarthi Srinivasan, Cynthia Cockerham-Morris, Aric Schadler, John Anthony Bauer, John O'Brien
Activation Of The Renin–Angiotensin–Aldosterone System Is Attenuated In Hypertensive Compared With Normotensive Pregnancy, Robin C. Shoemaker, Marko Poglitsch, Hong Huang, Katherine Vignes, Aarthi Srinivasan, Cynthia Cockerham-Morris, Aric Schadler, John Anthony Bauer, John O'Brien
UK CARES Faculty Publications
Hypertension during pregnancy increases the risk of adverse maternal and fetal outcomes, but the mechanisms of pregnancy hypertension are not precisely understood. Elevated plasma renin activity and aldosterone concentrations play an important role in the normal physiologic adaptation to pregnancy. These effectors are reduced in patients with pregnancy hypertension, creating an opportunity to define the features of the renin–angiotensin–aldosterone system (RAAS) that are characteristic of this disorder. In the current study, we used a novel LC-MS/MS-based methodology to develop comprehensive profiles of RAAS peptides and effectors over gestation in a cohort of 74 pregnant women followed prospectively for the development …
Terrain And Adversary-Aware Autonomous Robot Navigation, Aniekan Ufot Inyang
Terrain And Adversary-Aware Autonomous Robot Navigation, Aniekan Ufot Inyang
Electronic Theses and Dissertations
In autonomous robot navigation, the robot is able to understand the environment around it for intelligent navigation. From its world model of this environment, it generates a global plan for navigation from a position to a goal based on different factors. This research aims to implement autonomous robot navigation by learning terrain affordances: traversability (moving quickly) and concealment (staying hidden from an adversary) using the Preference-based Inverse Reward Learning (PbIRL) methodology. The PbIRL methodology reduces the barrier of generating initial demonstration data to learn the terrain affordances by using a human expert’s preferences to learn individual weights over the terrain …
Generalized Double Statistical Convergence Sequences On Ideals In Neutrosophic Normed Spaces, Jeyaraman M., Iswariya S., Pandiselvi R.
Generalized Double Statistical Convergence Sequences On Ideals In Neutrosophic Normed Spaces, Jeyaraman M., Iswariya S., Pandiselvi R.
Neutrosophic Systems with Applications
In this present research, having view in the Neutrosophic norm (u, v, w), which we presented I2-lacunary statistical convergence and I2-lacunary convergence strongly, looked into interactions between them, and made a few findings regarding the respective categories. At least went further to look at how both of such case approaches relate to I2-statistical convergence within the relevant Neutrosophic normed space.
Ber Analysis Of Bpsk Modulation Scheme For Multiple Combining Schemes Over Flat Fading Channel, Zuhaib Nishtar, Jamil Afzal
Ber Analysis Of Bpsk Modulation Scheme For Multiple Combining Schemes Over Flat Fading Channel, Zuhaib Nishtar, Jamil Afzal
Neutrosophic Systems with Applications
Focus of the study was to provide error-free communication in mobile communication with higher data rates, spectral efficiency, and energy efficient. Basically, work was done to investigate the performance of the Binary Phase Shift Keying (BPSK) modulation technique for multiple combining schemes and the behavior of signal in wireless communication where multipath propagation and uncertainty in the system. We use the Multiple-input Multiple-output (MIMO) and antenna diversity to get many copies of the same signal; some of them were faded, but some had sufficient information. Then the next step was to combine or select the best signal to achieve the …
Site-Specific Glyco-Engineering Of Recombinant Thrombomodulin, Joseph M. Keil
Site-Specific Glyco-Engineering Of Recombinant Thrombomodulin, Joseph M. Keil
ETD Archive
Thrombomodulin (TM), a membrane glycoprotein predominately expressed on endothelium, plays essential roles in regulating local hemostatic balance. Particularly, TM modulates the activity of thrombin from a procoagulant to an anticoagulant protease. When bound to TM, thrombin activates plasma protein C, which selectively inactivates coagulation factors Va and VIIIa. At the same time, TM-bound thrombin is unable to cleave fibrinogen or to activate platelets diminishing its procoagulant activity. recombinant TM456 is a promising anticoagulant candidate, but it has a very short half-life. TM is a proteoglycan and its O-linked glycosaminoglycan (GAG), chondroitin sulfate (CS), on the D3 domain contributes to TM’s …
Verification Of Astm Centrifugation Equations For Soil Suction Determination, Esteban Maclein Miranda Pinzon
Verification Of Astm Centrifugation Equations For Soil Suction Determination, Esteban Maclein Miranda Pinzon
Graduate Theses and Dissertations
The unit correction constant (B) in the ASTM D6836 standard needs to be evaluated. The B-value was proposed to be 0.00548 instead of 0.00553. This apparent discrepancy led to an investigation comparing the predicted and measured suction values for high air entry (HAE) ceramic disks. A Beckman-Coulter Model J6-MI six-bay centrifuge and a WP4C Dewpoint Potentiameter were used in the testing program. HAE ceramic disks rated with bubbling pressures of 3-bar and 5-bar were used as analogue soil specimens. The HAE ceramic disks were spun in the small-scale centrifuge to apply prescribed suction amounts and then placed in the WP4C …
Visualizing Transaction-Level Modeling Simulations Of Deep Neural Networks, Nataniel Farzan, Emad Arasteh
Visualizing Transaction-Level Modeling Simulations Of Deep Neural Networks, Nataniel Farzan, Emad Arasteh
Engineering Technical Reports
The growing complexity of data-intensive software demands constant innovation in computer hardware design. Performance is a critical factor in rapidly evolving applications such as artificial intelligence (AI). Transaction-level modeling (TLM) is a valuable technique used to represent hardware and software behavior in a simulated environment. However, extracting actionable insights from TLM simulations is not a trivial task. We present Netmemvisual, an interactive, cross-platform visualization tool for exposing memory bottlenecks in TLM simulations. We demonstrate how Netmemvisual helps system designers rapidly analyze complex TLM simulations to find memory contention. We describe the project’s current features, experimental results with two state-of-the-art deep …
Understanding The Role Of Interactivity And Explanation In Adaptive Experiences, Lijie Guo
Understanding The Role Of Interactivity And Explanation In Adaptive Experiences, Lijie Guo
All Dissertations
Adaptive experiences have been an active area of research in the past few decades, accompanied by advances in technology such as machine learning and artificial intelligence. Whether the currently ongoing research on adaptive experiences has focused on personalization algorithms, explainability, user engagement, or privacy and security, there is growing interest and resources in developing and improving these research focuses. Even though the research on adaptive experiences has been dynamic and rapidly evolving, achieving a high level of user engagement in adaptive experiences remains a challenge. %????? This dissertation aims to uncover ways to engage users in adaptive experiences by incorporating …
Generalizable Deep-Learning-Based Wireless Indoor Localization, Ali Owfi
Generalizable Deep-Learning-Based Wireless Indoor Localization, Ali Owfi
All Theses
The growing interest in indoor localization has been driven by its wide range of applications in areas such as smart homes, industrial automation, and healthcare. With the increasing reliance on wireless devices for location-based services, accurate estimation of device positions within indoor environments has become crucial. Deep learning approaches have shown promise in leveraging wireless parameters like Channel State Information (CSI) and Received Signal Strength Indicator (RSSI) to achieve precise localization. However, despite their success in achieving high accuracy, these deep learning models suffer from limited generalizability, making them unsuitable for deployment in new or dynamic environments without retraining. To …
Seek And Classify: End-To-End Joint Multi-Signal Detection And Classification Using Deep Learning, Prashant Subedi
Seek And Classify: End-To-End Joint Multi-Signal Detection And Classification Using Deep Learning, Prashant Subedi
School of Computing: Dissertations, Theses, and Student Research
The rise in the use of wireless communication has led to the problem of spectrum scarcity in licensed bands. The popularity of Internet of Things (IoT) requires innovative solutions that maximize the use of available spectrum to support the increasing number of connected devices. This thesis tackles two significant problems in wireless communication: the need for efficient spectrum sensing techniques and the scarcity of large, diverse raw in-phase (I) and quadrature (Q) datasets.
The ability to detect and classify modulation of the signals efficiently can enable a cognitive radio to monitor the spectrum activity in real time and utilize unused …
Proceedings Of The 2023 Berry Summer Thesis Institute, University Of Dayton. University Honors Program
Proceedings Of The 2023 Berry Summer Thesis Institute, University Of Dayton. University Honors Program
Proceedings of the Berry Summer Thesis Institute (2013-Present)
Thanks to a gift from the Berry Family Foundation and the Berry family, the University Honors Program launched the Berry Summer Thesis Institute in 2012. The institute introduces students in the University Honors Program to intensive research, scholarship opportunities and professional development. Each student pursues a 12-week summer thesis research project under the guidance of a UD faculty mentor. This contains the product of the students' research.
Contents:
- “How Porous Materials Affect the Boundary-Layer Transition of Hypersonic Flight Vehicles” (Megan C. Sieve)
- “Ultra-Stretchable, Self-Healing, DLP 3D-Printed Elastomers for Damage-Resistant Soft Robots: A Review” (Robert M. A. Drexler)
- “Extrapolation of Scalar …
All Hands On Deck: Choosing Virtual End Effector Representations To Improve Near Field Object Manipulation Interactions In Extended Reality, Roshan Venkatakrishnan
All Hands On Deck: Choosing Virtual End Effector Representations To Improve Near Field Object Manipulation Interactions In Extended Reality, Roshan Venkatakrishnan
All Dissertations
Extended reality, or "XR", is the adopted umbrella term that is heavily gaining traction to collectively describe Virtual reality (VR), Augmented reality (AR), and Mixed reality (MR) technologies. Together, these technologies extend the reality that we experience either by creating a fully immersive experience like in VR or by blending in the virtual and "real" worlds like in AR and MR.
The sustained success of XR in the workplace largely hinges on its ability to facilitate efficient user interactions. Similar to interacting with objects in the real world, users in XR typically interact with virtual integrants like objects, menus, windows, …
Healthcare Built Environment And The Covid-19 Pandemic, A Mixed-Method Study, Mohammad Saleh Nikooopayan Tak
Healthcare Built Environment And The Covid-19 Pandemic, A Mixed-Method Study, Mohammad Saleh Nikooopayan Tak
All Theses
The outbreak of COVID-19 presented an unparalleled risk to public health, leading to urgent scientific efforts in understanding transmission mechanisms and mitigating risks, especially in healthcare settings. This mixed-methods study examines knowledge advancement regarding indoor air quality in healthcare facilities during the pandemic. A systematic literature review of 163 post-COVID studies identifies key research thrusts and 31 recommendations related to building ventilation, air filtration, surface disinfection, and monitoring. Comparison with over 200 pre-pandemic publications reveals both reliance on established infection control principles and unique contributions, such as detecting viral RNA in air and surface samples indicating potential airborne and fomite …
A Unit-Load Approach For Reliability-Based Design Optimization Of Linear Structures Under Random Loads And Boundary Conditions, Robert James Haupin, Gene Jean-Win Hou
A Unit-Load Approach For Reliability-Based Design Optimization Of Linear Structures Under Random Loads And Boundary Conditions, Robert James Haupin, Gene Jean-Win Hou
Mechanical & Aerospace Engineering Faculty Publications
The low order Taylor’s series expansion was employed in this study to estimate the reliability indices of the failure criteria for reliability-based design optimization of a linear static structure subjected to random loads and boundary conditions. By taking the advantage of the linear superposition principle, only a few analyses of the structure subjected to unit-loads are needed through the entire optimization process to produce acceptable results. Two structural examples are presented in this study to illustrate the effectiveness of the proposed approach for reliability-based design optimization: one deals with a truss structure subjected to random multiple point constraints, and the …
Investigation Of The Flow Field In The Jack Rabbit Ii Mock Urban Environment Field Tests Using A 1:50 Scale Wind Tunnel Model, Paulo Victor De Freitas Lopes
Investigation Of The Flow Field In The Jack Rabbit Ii Mock Urban Environment Field Tests Using A 1:50 Scale Wind Tunnel Model, Paulo Victor De Freitas Lopes
Graduate Theses and Dissertations
Accidental release of toxic chemicals can put workers and nearby populations at significant risk. Consequence assessment of accidents in urban environments is of particular interest. Urban geometries create wind channels between buildings, along with particularly dangerous areas downwind of buildings, where near-stagnant flow is present. Furthermore, recirculation zones can be formed between buildings, trapping high concentrations of toxic gases at ground level, particularly for denser-than-air gases. In 2015, the Department of Homeland Security conducted the Jack Rabbit II Field Test (JR-II) at Dugway Proving Ground, UT. During JR-II, chlorine was released at the center of a Mock Urban Environment (MUE) …
Characteristics Of Refractivity And Sea State In The Marine Atmospheric Surface Layer And Their Influence On X-Band Propagation, Douglas Matthew Pastore
Characteristics Of Refractivity And Sea State In The Marine Atmospheric Surface Layer And Their Influence On X-Band Propagation, Douglas Matthew Pastore
Electronic Theses and Dissertations
Predictions of environmental conditions within the marine atmospheric surface layer (MASL) are important to X-band radar system performance. Anomalous propagation occurs in conditions of non-standard atmospheric refractivity, driven by the virtually permanent presence of evaporation ducts (ED) in marine environments. Evaporation ducts are commonly characterized by the evaporation duct height (EDH), evaporation duct strength, and the gradients below the EDH, known as the evaporation duct curvature. Refractivity, and subsequent features, are estimated in the MASL primarily using four methods: in-situ measurements, numerical weather and surface layer modeling, boundary layer theory, and inversion methods.
The existing refractivity estimation techniques often assume …
Evaluating The Feasibility Of Using Strain Measured During Sinusoidal Rate Pumping Tests To Characterize An Aquifer, Riley Blais
Evaluating The Feasibility Of Using Strain Measured During Sinusoidal Rate Pumping Tests To Characterize An Aquifer, Riley Blais
All Theses
Pumping tests with sinusoidal variation in pumping rate have been proposed as a method for improving aquifer characterization. These tests can interrogate a larger aquifer volume than slug tests and they can be more sensitive to small variations in drawdown. Current methods of using sinusoidal variations of rate are based on measuring pressure signals from the reservoir or aquifer, which requires access to monitoring wells. An alternative approach has been developed that measures the strain in the vadose zone instead of pressure in the reservoir. An instrument has been developed at Clemson University that can measure small strains using optical …
Phase Distribution Of Nickel, Chromium, And Cobalt In Seawater At Olivine-Enriched Beaches: Implications For Carbon Capture By Enhanced Silicate Weathering, Nathaniel J. Padrnos
Phase Distribution Of Nickel, Chromium, And Cobalt In Seawater At Olivine-Enriched Beaches: Implications For Carbon Capture By Enhanced Silicate Weathering, Nathaniel J. Padrnos
Master's Theses
The application of olivine to coastal areas is a proposed method of removing carbon dioxide from the atmosphere for climate change mitigation. Olivine is abundant and dissolves relatively fast, but it contains trace amounts of nickel (Ni), chromium (Cr), and cobalt (Co) which may be toxic to marine biota in cases of widespread coastal olivine spreading. Prior studies have suggested that Ni concentrations in marine sediments and/or the overlying water column due to olivine dissolution could be a limiting factor for its carbon capture potential. Therefore, it is critical to understand and model the processes affecting trace metal bioavailability after …
Experimental Study Of Linux Flightsize Estimation, Mingrui Zhang
Experimental Study Of Linux Flightsize Estimation, Mingrui Zhang
School of Computing: Dissertations, Theses, and Student Research
Transmission Control Protocol (TCP) is a fundamental Internet protocol responsible for controlling and coordinating the Internet traffic. As a result, TCP significantly influences the overall performance and stability of the Internet. One critical information required by a TCP connection to make decisions is FlightSize, which is the total amount of outstanding data contributed by the connection to the Internet. The FlightSize information is used by a TCP connection to determine its future sending rate and also avoid traffic congestion and collapse in the Internet. Consequently, an inaccurate estimation of FlightSize can result in degraded performance and instability of the Internet. …
A Data-Driven Multi-Regime Approach For Predicting Real-Time Energy Consumption Of Industrial Machines., Abdulgani Kahraman
A Data-Driven Multi-Regime Approach For Predicting Real-Time Energy Consumption Of Industrial Machines., Abdulgani Kahraman
Electronic Theses and Dissertations
This thesis focuses on methods for improving energy consumption prediction performance in complex industrial machines. Working with real-world industrial machines brings several challenges, including data access, algorithmic bias, data privacy, and the interpretation of machine learning algorithms. To effectively manage energy consumption in the industrial sector, it is essential to develop a framework that enhances prediction performance, reduces energy costs, and mitigates air pollution in heavy industrial machine operations. This study aims to assist managers in making informed decisions and driving the transition towards green manufacturing. The energy consumption of industrial machinery is substantial, and the recent increase in CO2 …
High-Power Laser Cooling And Temperature-Dependent Fluorescence Studies Of Ytterbium Doped Silica, Brian Topper
High-Power Laser Cooling And Temperature-Dependent Fluorescence Studies Of Ytterbium Doped Silica, Brian Topper
Optical Science and Engineering ETDs
Experimental observation of optical refrigeration using ytterbium doped silica glass in recent years has created a new solution for heat mitigation in high-power laser systems, nonlinear fiber experiments, integrated photonics, and precision metrology. Current efforts of different groups focus on compositional optimization, fiber fabrication, and investigating how much silica can be cooled with a laser. At the start of this work, the best effort in laser cooling ytterbium doped silica saw cooling by 6 K from room temperature. This dissertation follows the experimental efforts that culminated in the increase of this initial record by one order of magnitude. Comprehensive spectroscopic …
Heterochiral Dna Nanotechnology For Biomedical Applications, Tracy L. Mallette
Heterochiral Dna Nanotechnology For Biomedical Applications, Tracy L. Mallette
Biomedical Engineering ETDs
In the past 30 years, there have been major advancements on how to treat and diagnose disease because of the improvement and increase in accessibility of sequencing technology. Nucleic acid-based therapeutics can manipulate protein expression. Likewise, pathogens can be identified and detected with single nucleotide specificity. However, the underlying oligonucleotide technology requires protection against natural defense systems that have evolved to destroy foreign nucleic acids. Many chemical modifications that can protect nucleotides also have significant cytotoxic side effects and must be carefully designed into the strands. A novel way to protect against nuclease-mediated degradation is through the use of mirror-image, …
Integrating Sensor Development, Risk Assessment, And Community Engagement To Support Environmental Justice In The Rural Community Of El Tiple, Colombia, David Bahamon Pinzon
Integrating Sensor Development, Risk Assessment, And Community Engagement To Support Environmental Justice In The Rural Community Of El Tiple, Colombia, David Bahamon Pinzon
All Dissertations
In Colombia, ethnic communities have traditionally been responsible stewards of natural resources. They recognize the importance of these resources for their livelihood, as well as their ancestral and cultural heritage. El Tiple, a rural Afro-Colombian community, has been affected by the incursion of private corporations that promoted the expansion of sugarcane monocrops in their territory. Since the introduction of the monoculture industry, local freshwater sources have been depleted due to intensive water use for irrigation of the sugarcane crops. Additionally, the intensive usage of agrochemicals has been linked with loss of native flora, damages to family farms, and pollution of …
Facial Expression Recognition Using Convolutional Neural Networks (Cnns) And Generative Adversarial Networks (Gans) For Data Augmentation And Image Generation, Shekhar Singh
UNLV Theses, Dissertations, Professional Papers, and Capstones
Facial expressions play a crucial role in human communication, serving as a powerful means to convey emotions. However, classifying facial expressions using artificial intelligence (AI) can be challenging, especially with small datasets and images. Facial Expression Recognition (FER) is an active area of research, with Convolutional Neural Networks (CNNs) being widely employed for classification. In this research, we propose a CNN-based approach for FER that utilizes both original and augmented datasets to enhance classification accuracy. Experimental results on the FER2013 dataset show test accuracies of 63.39% and 64.59% for the original and augmented datasets, respectively, in a seven-class classification task. …
Phosphatase And Pseudo-Phosphatase Functions Of Phosphatase Of Regenerating Liver 3 (Prl-3) Are Insensitive To Divalent Metals In Vitro, Jeffery T. Jolly, Ty C. Cheatham, Jessica S. Blackburn
Phosphatase And Pseudo-Phosphatase Functions Of Phosphatase Of Regenerating Liver 3 (Prl-3) Are Insensitive To Divalent Metals In Vitro, Jeffery T. Jolly, Ty C. Cheatham, Jessica S. Blackburn
Markey Cancer Center Faculty Publications
Phosphatase of regenerating liver 3 (PRL-3) is associated with cancer metastasis and has been shown to interact with the cyclin and CBS domain divalent metal cation transport mediator (CNNM) family of proteins to regulate the intracellular concentration of magnesium and other divalent metals. Despite PRL-3’s importance in cancer, factors that regulate PRL-3’s phosphatase activity and its interactions with CNNM proteins remain unknown. Here, we show that divalent metal ions, including magnesium, calcium, and manganese, have no impact on PRL-3’s structure, stability, phosphatase activity, or CNNM binding capacity, indicating that PRL-3 does not act as a metal sensor, despite its interaction …
Structures Of The Insecticidal Toxin Complex Subunit Xpta2 Highlight Roles For Flexible Domains, Cole L. Martin, David W. Chester, Christopher D. Radka, Lurong Pan, Zhengrong Yang, Rachel C. Hart, Elad M. Binshtein, Zhao Wang, Lisa Nagy, Lawrence J. Delucas, Stephen G. Aller
Structures Of The Insecticidal Toxin Complex Subunit Xpta2 Highlight Roles For Flexible Domains, Cole L. Martin, David W. Chester, Christopher D. Radka, Lurong Pan, Zhengrong Yang, Rachel C. Hart, Elad M. Binshtein, Zhao Wang, Lisa Nagy, Lawrence J. Delucas, Stephen G. Aller
Markey Cancer Center Faculty Publications
The Toxin Complex (Tc) superfamily consists of toxin translocases that contribute to the targeting, delivery, and cytotoxicity of certain pathogenic Gram-negative bacteria. Membrane receptor targeting is driven by the A-subunit (TcA), which comprises IgG-like receptor binding domains (RBDs) at the surface. To better understand XptA2, an insect specific TcA secreted by the symbiont X. nematophilus from the intestine of entomopathogenic nematodes, we determined structures by X-ray crystallography and cryo-EM. Contrary to a previous report, XptA2 is pentameric. RBD-B exhibits an indentation from crystal packing that indicates loose association with the shell and a hotspot for possible receptor binding or a …