Engineering Commons^™

Generation Of Recombinant Mouse Embryonic Stem Cell Lines And Theirapplication For In Vivo Bioluminiscence Imaging In The Heart, Ramana Kammili

Electronic Theses and Dissertations

Cardiovascular disease is the major cause of death in the United States, with 80 million people suffering from some form of heart disease each year. One major limitation is the inability of the heart to repair the damaged tissue. Stem cell therapy holds enormous promise to repair and regenerate the damaged myocardium, but there are many technical difficulties that must first be overcome. One such difficulty is the present lack of ability to track and assess transplanted stem cells over time in vivo. The central hypothesis of this thesis is that in vivo bioluminescence imaging is a safe and useful …

Santa Clara Magazine, Volume 50 Number 3, Winter 2008, Santa Clara University

Santa Clara Magazine

12 - ONE GOLD...AND A HOST OF MEMORIES By Ann Killion. A Beijing Olympic scrapbook.

16 - GOD AND WOMAN AT YALE By Steven Boyd Saum. For more than 300 years, the role of Yale University’s chaplain has never been filled by a woman, a layperson, or a Catholic—until now. Meet Sharon M. K. Kugler ’81.

20 - WITH RENEWED VIGOR AND ZEAL By Michael G. Boughton, S.J. How six decrees define the mission of the Society of Jesus in the years to come.

22 - LIBERTY AND THE PURSUIT By Michael S. Malone ’75, MBA ’77. Without meaning to, …

A Surface Inspection Machine Vision System That Includes Fractal Texture Analysis, Jonathan Blackledge, Dmitry Dubovitskiy

Articles

The detection, recognition and classification of features in a digital image is an important component of quality control systems in production and process engineering and industrial systems monitoring, in general. In this paper, a new pattern recognition system is presented that has been designed for the specific task of monitoring the quality of sheet-steel production in a rolling mill. The system is based on using both the Euclidean and Fractal geometric properties of an imaged object to develop training data that is used in conjunction with a supervised learning procedure based on the application of a fuzzy inference engine. Thus, …

Patient-Centred Laboratory Validation Using Software Agents, John Mcgrory, Jane Grimson, Frank Clarke, Peter Gaffney

Conference Papers

Guidelines are self-contained documents which healthcare professionals reference to obtain knowledge about a specific condition or process. They interface with these documents and apply known facts about specific patients to gain useful supportive information to aid in developing a diagnosis or manage a condition. To automate this process a series of Standard Operating Procedures (SOP) and workflow processes are constructed using the contents of these documents in order to manage the validation flow of a patient sample. These processes decompose the guidelines into workflow plans, which are then called using condition triggers controlled by a centralised management engine. The software …

Detailed Characterization Of The Mouse Embryonic Stem Cell Transcriptome Reveals Novel Genes And Intergenic Splicing Associated With Pluripotency, Galih Kunarso, Kee-Yew Wong, Lawrence W. Stanton, Leonard Lipovich

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Transcriptional control of embryonic stem (ES) cell pluripotency has been a subject of intense study. Transcriptional regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state. However, the ES cell transcriptome is not limited to their targets, and exhibits considerable complexity when assayed with microarray, MPSS, cDNA/EST sequencing, and SAGE technologies. To identify novel genes associated with pluripotency, we globally searched for ES transcripts not corresponding to known genes, validated their sequences, determined their expression profiles, and employed RNAi to test their function.

Results

Gene Identification Signature (GIS) analysis, a SAGE derivative distinguished …

Rule-Based Mamdani-Type Fuzzy Modeling Of Skin Permeability, Deepak R. Keshwani, David D. Jones, George E. Meyer, Rhonda M. Brand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Two Mamdani type fuzzy models (three inputs–one output and two inputs–one output) were developed to predict the permeability of compounds through human skin. The models were derived from multiple data sources including laboratory data, published data bases, published statistical models, and expert opinion. The inputs to the model include information about the compound (molecular weight and octonal–H₂O partition coefficient) and the application temperature. One model included all three parameters as inputs and the other model only included information about the compound. The values for mole molecular weight ranged from 30 to 600 Da. The values for the log …

An Efficient Algorithm For Biomarker Identification, Jiang Li, Rick Mckenzie, Lisa Cazares, Richard Drake, John Semmens

Electrical & Computer Engineering Faculty Publications

No abstract provided.

Object Detection And Classification With Applications To Skin Cancer Screening, Jonathan Blackledge, Dmitryi Dubovitskiy

Articles

This paper discusses a new approach to the processes of object detection, recognition and classification in a digital image. The classification method is based on the application of a set of features which include fractal parameters such as the Lacunarity and Fractal Dimension. Thus, the approach used, incorporates the characterisation of an object in terms of its texture.

The principal issues associated with object recognition are presented which includes two novel fast segmentation algorithms for which C++ code is provided. The self-learning procedure for designing a decision making engine using fuzzy logic and membership function theory is also presented and …

N-Glycan Modification In Aspergillus Species, Elke Kainz, Andreas Gallmetzer, Christian Hatzl, Juergen H. Nett, Huijuan Li, Thorsten Schinko, Robert Pachlinger, Harald Berger, Yazmid Reyes-Dominguez, Andreas Bernreiter, Tillmann Gerngross, Stefan Wildt, Joseph Strauss

Dartmouth Scholarship

The production by filamentous fungi of therapeutic glycoproteins intended for use in mammals is held back by the inherent difference in protein N-glycosylation and by the inability of the fungal cell to modify proteins with mammalian glycosylation structures. Here, we report protein N-glycan engineering in two Aspergillus species. We functionally expressed in the fungal hosts heterologous chimeric fusion proteins containing different localization peptides and catalytic domains. . This strategy allowed the isolation of a strain with a functional -1,2-mannosidase producing increased amounts of N-glycans of the Man 5 GlcNAc 2 type. This strain was further engineered by the introduction of …

Moment Analysis Of Near-Equilibrium Binding Interactions During Electrophoresis, Yousef Daneshbod, James D. Sterling, Ali Nadim

Business and Information Technology Faculty Research & Creative Works

The electrophoretic transport of three chemically reacting species, two of which can bind reversibly to form the third, is analyzed mathematically. The species are assumed to move horizontally through a long channel with different electrophoretic mobilities and diffusion coefficients. By considering small perturbations of the system about equilibrium or when one of the two binding species is much more abundant than the other, the governing advection-reaction- diffusion equations can be linearized and studied via the method of moments. The result is a set of coupled ordinary differential equations for the moments that can be solved analytically. Analysis of the long-time …

Flight Physician - November, 2007, Civil Aviation Medical Association

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A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

2007 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library, Joanne E. Goodell Ph.D.

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Dr. Joanne Goodell was the guest speaker.

Santa Clara Magazine, Volume 49 Number 2, Fall 2007, Santa Clara University

Santa Clara Magazine

14 - A DAY IN THE LIFE OF THE PRESIDENT By Ron Hansen. Set your alarm clock early-then get up and follow Paul Locatelli, S.J., through a day of leading the University and serving as pastor, professor, mayor, and CEO.

22 - BUILT BY IMMIGRANTS By Gerald McKevitt, S.J. How Italian Jesuits helped shape the American West, from religious devotions to curriculum to pasta.

28 - YOU ARE HERE By Sarah Stanek. SCU students and faculty collaborate on a groundbreaking project documenting early life at Mission Santa Clara-and the result is a book that's the first of its kind for …

Microfabrication Of An Optical Chamber For Exposing Living Cells To Pulsed Electric Fields, Saleh Sheikh

Electrical & Computer Engineering Theses & Dissertations

I have fabricated a chamber which enables the application of Nanosecond Pulsed Electric Fields (nsPEFs) to living cells while monitoring their real time fluorescence with an inverted microscope. The No. I thickness (150µm nominal) coverslip glass substrate used for fabrication allows the use of high numerical aperture oil immersion objectives for imaging intracellular organelles. Electrodes are fabricated by first evaporating a chromium adhesion layer, followed by a nickel seed layer. Subsequently, an ultra-thick positive photoresist is applied and patterned to create a 30µm thick electroplating mold. The electrodes are then electrodeposited into the mold from a nickel sulfamate plating bath. …

Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela D. Morris, Gayle B. Mccombs, Susan L. Tolle, Mounir Laroussi, Wayne L. Hynes

Dental Hygiene Faculty Publications

Cold plasma is a state of matter that contains a large number of particles that are electrically charged. Plasmas generate chemically reactive species and ultraviolet radiation making them useful in decontamination applications (Kong & Laroussi, 2003). Research regarding the inactivation of gram-positive bacteria by cold plasma has been studied by Laroussi et al (2003); however, there is limited research regarding the germicidal effectiveness of cold plasma on Geobacillus stearothermophilus and Bacillus cereus microorganisms. The purpose of this study was to determine if cold plasma technology inactivates Geobacillus stearothermophilus and Bacillus cereus vegetative cells and spores. This study consisted of 981 …

Simulation To Augment Standardized Patients In Obstetric Ultrasound Training, Scott Richard Guirlinger

Computational Modeling & Simulation Engineering Theses & Dissertations

Standardized patients are actors used in medical training to teach and eval11atc clinical skills of students prior to their using these skills on an actual patient. Augmented Reality is a relatively new technology that can be used to augment standardized patients with abnonnalitie8 and pathologies that they cannot portray themselves as normal healthy human beings. In our project, we sought to augment standardized patients with a 12-week- old fetus in order to train and possibly evaluate medical students and professionals in performing obstetric ultrasound procedures and interpreting the corresponding ultrasound image results.

The software library ARToolkit provided the framework for …

Receiver Operating Characteristic And Location Analysis Of Simulated Near-Infrared Tomography Images, Xiaomei Song, Brian W. Pogue, Hamid Dehghani, Shudong Jiang, Keith D. Paulsen, Tor D. Tosteson

Dartmouth Scholarship

Receiver operating characteristic (ROC) analysis was performed on simulated near-infrared tomography images, using both human observer and contrast-to-noise ratio (CNR) computational assessment, for application in breast cancer imaging. In the analysis, a nonparametric approach was applied for estimating the ROC curves. Human observer detection of objects had superior capability to localize the presence of heterogeneities when the objects were small with high contrast, with a minimum detectable threshold of CNR near 3.0 to 3.3 in the images. Human observers were able to detect heterogeneities in the images below a size limit of 4 mm, yet could not accurately find the …

Flight Physician - August, 2007, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …

Sheep Updates 2007 - Part 2, Ian Mcfarland, Catherine Stockman, Anne Barnes, David Pethick, Jenny Davis, Brett Thompson, Ron Leng, Sally Pieruzzini, Elizabeth Jackson, Mohammed Quaddus, Nazrul Islam, John Stanton, Melanie Dowling

Sheep Updates

This session covers six papers from different authors:

CONCURRENT SESSIONS

FINISHING LAMB AND BEEF

1. Precision Feedlot Lamb, Ian McFarland, Department of Agriculture and Food, Western Australia

2. Feeding sheep under high heat load did not decrease intake of feedlot rations, Catherine Stockman, Department of Agriculture and Food, Western Australia & Murdoch University, Anne Barnes, Murdoch University David Pethick, Murdoch University

3. Taking the stress out of fifishing lambs and cattle - EasyFeed solutions, Jenny Davis, Brett Thomson, Milne AgriGroup, Welshpool WA, Ron Leng, Emeritus Professor, University of New England, Armidale, NSW

WOOL

4. DAFWA …

Santa Clara Magazine, Volume 49 Number 1, Summer 2007, Santa Clara University

Santa Clara Magazine

8 - A CENTURY OF BRONCO BASKETBALL, By Jed Mettee and Steven Boyd Saum. Celebrating the first hundred seasons.

12 - A FAMILY SHOW By Steven Boyd Saum. Meet new men's basketball coach Kerry Keating.

14 - A SPACE THAT TALKS TO NATURE By Miriam Schulman. A team of SCU students aim to win the Department of Energy's Solar Decathlon. And save the planet.

19 - EPITAPH FOR THE JOURNEY A poem by Paul Mariani.

22 - THE BUZZ ABOUT SUSTAINABILITY By John Farnsworth. When we talk about "sustainability," what are we really talking about?

26 - THE GREEN TAX …

Nonlinear Dynamical Analysis Of Brain Electrical Activity Due To Exposure To Weak Environmentally Relevant Electromagnetic Fields, Erik Alfonso Nilsen

Doctoral Dissertations

The reports dealing with the effects of weak electromagnetic fields (EMFs) on brain electrical activity have been inconsistent. We suspected that the use of linear models and their associated methods accounted for some of the variability, and we explored the issue by using a novel approach to study the effects of EMFs on the electroencephalogram (EEG) from rabbits and humans. The EEG was embedded in phase space and local recurrence plots were calculated and quantified to permit comparisons between exposed and control epochs from individual subjects. Statistically significant alterations in brain activity were observed in each subject when exposed to …

Flight Physician - June, 2007, Civil Aviation Medical Association

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A twenty page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

2007 - The Twelfth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Twelfth Annual Symposium of Student Scholars, held on April 23, 2007. Includes abstracts from the presentations and posters.

Santa Clara Magazine, Volume 48 Number 4, Spring 2007, Santa Clara University

Santa Clara Magazine

8 - ARCHAEOLOGISTS OF THE CARIBBEAN By Monte Lorenzet. Eye patch and peg leg-we all know what makes a pirate. Or do we? Only recently have scientists taken a serious look at the archeology of piracy, with SCU's Russ Skowronek one of the hearties unearthing facts buried beneath centuries of myth.

12 - ARE PEOPLE GETTING CRAZIER? By Thomas G. Plante. From what the media offers every day, you can't help but think the world is going to hell in a handbasket. What's really going on? And what can we do about it?

21 - A TEACHABLE MOMENT Q&A with …

Simulation And Modeling Of Nerve Impulse Propagation And Its Blockage By External Electrical Stimulation, Nidhi Nahar

Electrical & Computer Engineering Theses & Dissertations

The electric nature of biological tissues permits the transmission of signals for information and for control and is, therefore, of vital importance for life. The latest scientific achievements and nanoscale probing techniques now allow scientists to research at the subcellular level by measuring the electric current flows through a single ion channel of the cell membrane with the patch-clamp method. With the latter approach, the effects of electrical stimulation can be quantified, understood, and applied to molecular biology and the development of medical diagnostic and therapeutic methods.

In this thesis, the Hodgkin-Huxley approach for unmyelinated fibers, coupled with a transmission …

Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela Dawn Morris

Dental Hygiene Theses & Dissertations

Non-equilibrium atmospheric pressure plasma, also known as cold plasma, is a state of matter that consists of a mix of neutral and charged particles. Plasma generates chemically reactive species and ultraviolet radiation making them useful in decontamination applications (Kong & Laroussi, 2003). Research regarding the inactivation of gram-positive bacteria, such as Bacillus atrophaeus, by cold plasma has been studied by Laroussi et al. (2003); however, there is limited research regarding the germicidal effectiveness of cold plasma on the microorganisms Geobacillus stearothermophilus and Bacillus cereus. The purpose of this study was to determine if cold plasma technology inactivates heat …

Condition Reporting And Resolution, J. M. Pesek, S. A. Wade

Publications (YM)

This procedure facilitates a safety conscious work environment by providing a mechanism for employees to make management aware of existing and potential conditions. This procedure establishes the responsibilities and process to be used to ensure that conditions related to, but not limited to, the environment, safety, health, waste isolation, operations, security, or quality of items and services associated with Office of Civilian Radioactive Waste Management (OCRWM) work activities are promptly identified, controlled, evaluated, and corrected as soon as practical. This procedure describes the process flow, controls, interfaces, and requirements for condition identification and resolution. This includes adverse conditions as well …

Flight Physician - March, 2007, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Accessibility Of Home Blood Pressure Monitors For Blind And Visually Impaired People, Mark M. Uslan, Darren M. Burton, Thomas E. Wilson, Steven Taylor, Bruce S. Chertow, Jack E. Terry

Physics Faculty Research

Background: The prevalence of hypertension comorbid with diabetes is a significant health care issue. Use of the home blood pressure monitor (HBPM) for aiding in the control of hypertension is noteworthy because of benefits that accrue from following a home measurement regimen. To be usable by blind and visually impaired patients, HBPMs must have speech output to convey all screen information, an easily readable visual display, identifiable controls that are easy to use, and an accessible user manual.

Methods: Data on the physical aspects and the features and functions of nine Food and Drug Administration-approved HBPMs (eight of which were …