Engineering Commons^™

Multiple And Consecutive Genome Editing Using I-Gonad And Breeding Enrichment Facilitates The Production Of Genetically Modified Mice, Carolina R Melo-Silva, Cory J Knudson, Lingjuan Tang, Samita Kafle, Lauren E. Springer, Jihae Choi, Christopher M. Snyder, Yajing Wang, Sangwon V. Kim, Luis J. Sigal

Department of Microbiology and Immunology Faculty Papers

Genetically modified (GM) mice are essential tools in biomedical research. Traditional methods for generating GM mice are expensive and require specialized personnel and equipment. The use of clustered regularly interspaced short palindromic repeats (CRISPR) coupled with improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) has highly increased the feasibility of producing GM mice in research laboratories. However, genetic modification in inbred mouse strains of interest such as C57BL/6 (B6) is still challenging because of their low fertility and embryo fragility. We have successfully generated multiple novel GM mouse strains in the B6 background while attempting to optimize i-GONAD. We found …

The Santa Clara, 2023-05-05, Santa Clara University

The Santa Clara

No abstract provided.

Redesigning Nasogastric Feeding Tubes To Prevent Dislodgement, Sydney Ruth Rester, Shelby Lynn Berry

Honors Theses

Seniors at the University of Mississippi studying biomedical engineering complete a senior project that seeks to solve a problem in healthcare. Our team, composed of Sydney Rester, Shelby Berry, Andrew Ulmer, and Alex Bromley, sought to improve care associated with nasogastric feeding tubes. We identified two major problems: clogging and tube dislodgement. To address the problem of dislodgement, the team crafted a new feeding tube design with a balloon attachment. The attachment prevents a patient from pulling the feeding tube out and keeps the tube from dislodging due to normal patient movement. We further determined that our tube should be …

Redesigning Nasogastric Feeding Tubes To Prevent Dislodgement, Shelby Lynn Berry, Sydney Ruth Rester

Honors Theses

Seniors at the University of Mississippi studying biomedical engineering complete a senior project that seeks to solve a problem in healthcare. Our team, composed of Sydney Rester, Shelby Berry, Andrew Ulmer, and Alex Bromley, sought to improve care associated with nasogastric feeding tubes. We identified two major problems: clogging and tube dislodgement. To address the problem of dislodgement, the team crafted a new feeding tube design with a balloon attachment. The attachment prevents a patient from pulling the feeding tube out and keeps the tube from dislodging due to normal patient movement. We further determined that our tube should be …

Dense & Attention Convolutional Neural Networks For Toe Walking Recognition, Junde Chen, Rahul Soangra, Marybeth Grant-Beuttler, Y. A. Nanehkaran, Yuxin Wen

Physical Therapy Faculty Articles and Research

Idiopathic toe walking (ITW) is a gait disorder where children’s initial contacts show limited or no heel touch during the gait cycle. Toe walking can lead to poor balance, increased risk of falling or tripping, leg pain, and stunted growth in children. Early detection and identification can facilitate targeted interventions for children diagnosed with ITW. This study proposes a new one-dimensional (1D) Dense & Attention convolutional network architecture, which is termed as the DANet, to detect idiopathic toe walking. The dense block is integrated into the network to maximize information transfer and avoid missed features. Further, the attention modules are …

A Proof Of Concept For Detection Of Palato-Lingual Contact Without Intraoral Electrodes In A Human Participant, Amitava Biswas

Master's Theses

The author of this thesis conceived and investigated an unexpected electrical signal that may not be noticed during regular electroglottography (EGG) when electrodes are conventionally placed on the anterior surface of the throat. It appears that there is a measurable electrical signal from the EGG equipment when electrodes are placed over the frontal cheek (belly of zygomatic muscle), and the tongue is elevated to contact the hard palate, or the tongue is lowered to break the contact. Therefore, this phenomenon is designated as unconventional electroglottography (UEGG, Dr. Steven Cloud, private communication, July 17, 2022).

Two distinctive patterns of waveforms were …

Innovations In Football Protective Headgear, Henry Bethel

Civil Engineering Undergraduate Honors Theses

Adding impact reduction to football protective headgear decreases the reactionary acceleration experienced by the cranium. Utilizing an iteration of the National Operating Committee for Standards in Athletic Equipment (NOCSAE) method of testing per ASTM F429, a drop tower was fabricated, and 27 drop tests were conducted upon a standard helmet (control) and the Summerlin model helmet (test), measuring the maximum acceleration experienced by each drop. Drop tests involved variable heights and test angles with three drops being made per combination. The Summerlin model drops were conducted until failure, occurring at the third 3” drop at the “front” location at an …

Detecting Pathobiomes Using Machine Learning, Valerie Jackson, Valerie Jackson

Industrial Engineering Undergraduate Honors Theses

Machine learning is a field with high growth potential due to the overall continuous progressions, developments, advancements, and improvements caused by the way it is used to help interpret and use large amounts of data [1]. One type of data that can be collected and analyzed by these machine learning models is data that is associated with DNA and information that the DNA gives. The research will be focusing specifically on using machine learning technology to detect pathobiomes indicative of salmonella pork. The pathobiome associated with salmonella is very similar to others, and this causes a problem for classification/detection with …

An In Vivo Biocompatibility Analysis Of A Novel Tissue Regeneration Matrix Using A Pig Model, Shamar Thomas

All Theses

The goal of this project is to develop an injectable bead scaffold to promote tissue regeneration in the void created by lumpectomy and to alleviate post lumpectomy problems by preventing local recurrence and minimizing surgical-related infections. Microbeads were synthesized from collagen type I and crosslinked with tannic acid to form the basis for this injectable therapeutic. Tannic acid acts as a therapeutic anticancer agent. The action mechanisms of tannins in breast cancer cells have been studied with studies showing tannins to be cytotoxic to cancer cells in a dose-dependent manner. Tannic acid induces apoptosis in breast cancer cells via caspase …

Program And Proceedings: Nebraska Academy Of Sciences 1880–2023, 143rd Anniversary Year, One Hundred-Thirty-Third Annual Meeting, April 21, 2023

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Humans Past and Present

Applied Science and Technology Section

Biology

Biomedical Sciences

Chemistry

Earth Sciences

Environmental Science

Physics

Science Education

2023 Maiben Lecture: Jason Bartz

2023 Friend of Science Award: Ray Ward and Jim Lewis

Synthesis, Radiolabeling And Evaluation Of A Suite Of Tracers With 44Sc For Detecting Extracellular Dna, Zhiyao Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Neutrophil extracellular traps involve the rapid translocation of DNA to the outside of the cell under certain stimuli. This structure forms a fibrous network that is able to limit the spread of pathogens and to kill microorganisms. It has also been shown to be present in various pathological processes such as inflammation, autoimmune diseases, and cancer metastasis. Currently, the formation process of NETs in vivo is being extensively studied. However noninvasive detection and quantitation has yet to be achieved. A class of PET tracers are described here that consists of a DNA dye as the backbone that is labeled with …

Development Of A Computational Model To Investigate Pathways And The Effects Of Treatment In Fanconi Anemia, Sabrina Kellett

Biological Sciences Undergraduate Honors Theses

Fanconi Anemia (FA) is a rare type of anemia that is not easily studied and can have very detrimental effects. This disease compromises the bone marrow, resulting in decreased hemopoiesis. Symptoms of FA also include abnormalities in the brain and spinal cord, incorrect formation of the kidneys, abnormal formation of the heart and lungs, and a dramatically increased risk of developing cancer. FA can be caused by various mutations in any of the 22 genes that encode for proteins involved in what is called the FA DNA repair pathway. In healthy individuals, this pathway specifically repairs interstrand cross-links (ICLs) recognized …

Designing A Work-Life Balance Learning Module For Cal Poly Construction Management Department, Alex Jones

Construction Management

The construction industry is known to create a highly stressful work environment given its unpredictability and mental/time demanding nature, which creates challenges for professionals to maintain a healthy work-life balance. This senior project aims to research and identify the key areas that foster this stress and propose a learning module for the Cal Poly Construction Management Department to implement into class curriculum. Having this learning module taught to students will raise awareness of the topic for future industry professionals and equip them with the tools to maintain a healthy equilibrium between their work demands and personal life. The learning module …

Opensim-Based Musculoskeletal Modeling: Foundation For Interactive Obstetric Simulator, Bahador Dodge

Electrical & Computer Engineering Theses & Dissertations

The use of mathematical and computational models to understand complex biological systems, such as the human birth process, is a rapidly growing field in medicine. These models can be used to optimize and personalize medical treatments for individual patients, enhance training, and aid in educational efforts. While recent advancements in healthcare, particularly in obstetrics, have improved care for mothers and babies, studies and government reports indicate a rising rate of maternal mortality in the United States.

Despite this rising trend, there is a lack of detailed studies concerning the use of modeling and simulation to develop an interactive obstetrics simulator …

Development Of A Cost-Constrained Intelligent Prosthetic Knee With Real-Time Machine Learning, Predictive Stumble Control, Lucas Jonathan Galey

Open Access Theses & Dissertations

The field of biomechatronics is evolving quickly with advances in computer science, biology, and electrical and mechanical engineering. Coupled with increased interests in machine learning (ML) across all industry sectors, there are opportunities to leverage advanced analytics in uniquely complex problems. This study aimed to deploy real-time ML predictions in a novel microprocessor-controlled prosthetic knee (MPK) device capable of identifying and responding to stumble-events to reduce amputee fall prevalence. Innately, stumbling is a chaotic event. Current MPKs operate by detecting gait characteristics and reacting to preprogrammed states. While these systems are beneficial in significant ways, such as energy expenditure and …

The Impact Of Flight On Cabin Crew Wellness: A Literature Review, Ashleigh Roderick

Senior Honors Theses

Since its conception in 1903, aviation has captivated the attention of people across the globe and has been utilized in many sectors of society. Aviation related occupations pose a unique set of risks to those working in the field, health risks being one of the most prominent. Cancer, cardiovascular disease, musculoskeletal ailments, and genetic damage have all been studied as potential consequences of working in the aviation industry, as some studies indicate a higher prevalence of these issues in aviation employees. Apart from physiological issues, mental health issues have also been observed in those working in aviation. Mental health issues …

Cortical Effects Of Wrist Tendon Vibration During An Arm Tracking Task In Chronic Stroke Survivors: An Eeg Study, Dylan B. Snyder, Scott A. Beardsley, Allison S. Hyngstrom, Brian D. Schmit

Biomedical Engineering Faculty Research and Publications

The purpose of this study was to characterize changes in cortical activity and connectivity in stroke survivors when vibration is applied to the wrist flexor tendons during a visuomotor tracking task. Data were collected from 10 chronic stroke participants and 10 neurologically-intact controls while tracking a target through a figure-8 pattern in the horizontal plane. Electroencephalography (EEG) was used to measure cortical activity (beta band desynchronization) and connectivity (beta band task-based coherence) with movement kinematics and performance error also being recorded during the task. All participants came into our lab on two separate days and performed three blocks (16 trials …

Enhanced Iot-Based Electrocardiogram Monitoring System With Deep Learning, Jian Ni

UNLV Theses, Dissertations, Professional Papers, and Capstones

Due to the rapid development of computing and sensing technologies, Internet of Things (IoT)-based cardiac monitoring plays a crucial role in providing patients with cost-efficient solutions for long-term, continuous, and pervasive electrocardiogram (ECG) monitoring outside a hospital setting. In a typical IoT-based ECG monitoring system, ECG signals are picked up by sensors located on the edge, and then uploaded to the remote cloud servers. ECG interpretation is performed for the collected ECGs in the cloud servers and the analysis results can be made instantly available to the patients as well as their healthcare providers.In this dissertation, we first examine the …

Preparing Homogenous Composites Of Collagen And Cellulose Nanocrystals For Tissue Engineering Research, Zachary Stanley

Biological and Agricultural Engineering Undergraduate Honors Theses

Advancements in medicine and our understanding of stem cells have led to a greater emphasis on further developing research focused on tissue engineering. This research has led to the rise of both two-dimensional and three-dimensional scaffolds that can be utilized to repair bone, skin, vascular, and potentially even nervous tissue. One of the prominent compounds used in modern scaffolds is collagen-based hydrogels due to their low antigenicity and ability to provide structure to cells. There is potential to further improve upon this three-dimensional scaffold by incorporating cellulose nanocrystals (CNCs) into a composite hydrogel with collagen. The addition would increase the …

Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao

Michigan Tech Publications, Part 1

Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …

Digital Patient Engagement At A Perioperative Surgical Home Implemented Community Hospital, Srinivasan Sridhar, Amy Mount Hunter, Bernadette Mccrory

Patient Experience Journal

Patients in rural areas typically require more perioperative ‘optimization’ for surgery. The rural healthcare systems often overwhelmed with coordinating perioperative services and deliver less than optimal surgical outcomes. This is due to limited supporting microsystems and ability to effectively engage and track patients over the 120-day perioperative period to limit post-surgical complications. The study assessed longitudinal patient engagement within a newly established Perioperative Surgical Home (PSH) at a rural community hospital serving 10+ surrounding counties to identify barriers and best practices for engagement. A digital patient engagement platform was implemented and used to assess longitudinal patient outcomes and engagement from …

Regenerative Medicine For Tendon/Ligament Injuries: De Novo Equine Tendon/Ligament Neotissue Generation And Application, Takashi Taguchi

LSU Doctoral Dissertations

Tendon and ligament injuries are debilitating conditions across species. Poor regenerative capacities of these tissues limit restoration of original functions. The first study evaluated the effect of cellular administration on tendon/ligament injuries in horses using meta-analysis. The cellular administration was effective in restoring ultrasonographic echogenicity and increasing vascularity during early phase of healing. Additionally, it improved microstructural organization of healed tissue in terms of cellularity and fiber alignment. However, the study did not support its use for increasing rate of return to performance, expression/deposition of tendon-specific genes/proteins, or mechanical properties.

The findings led to the second study that engineered implantable …

Analysis Of The Adherence Of Mhealth Applications To Hipaa Technical Safeguards, Bilash Saha

Master of Science in Information Technology Theses

The proliferation of mobile health technology, or mHealth apps, has made it essential to protect individual health details. People now have easy access to digital platforms that allow them to save, share, and access their medical data and treatment information as well as easily monitor and manage health-related issues. It is crucial to make sure that protected health information (PHI) is effectively and securely transmitted, received, created, and maintained in accordance with the rules outlined by the Health Insurance Portability and Accountability Act (HIPAA), as the use of mHealth apps increases. Unfortunately, many mobile app developers, particularly those of mHealth …

The Santa Clara, 2023-04-21, Santa Clara University

The Santa Clara

No abstract provided.

Recent Advances In Antimicrobial Peptide Hydrogels, Aryanna Copling, Maxwell Akantibila, Raaha Kumaresan, Gilbert Fleischer, Dennise Cortes, Rahul S Tripathi, Valerie J. Carabetta, Sebastian Vega

Cooper Medical School of Rowan University Departmental Research

Advances in the number and type of available biomaterials have improved medical devices such as catheters, stents, pacemakers, prosthetic joints, and orthopedic devices. The introduction of a foreign material into the body comes with a risk of microbial colonization and subsequent infection. Infections of surgically implanted devices often lead to device failure, which leads to increased patient morbidity and mortality. The overuse and improper use of antimicrobials has led to an alarming rise and spread of drug-resistant infections. To overcome the problem of drug-resistant infections, novel antimicrobial biomaterials are increasingly being researched and developed. Hydrogels are a class of 3D …

Enhancement Of Deep Learning Protein Structure Prediction, Ruoming Shen

Modeling, Simulation and Visualization Student Capstone Conference

Protein modeling is a rapidly expanding field with valuable applications in the pharmaceutical industry. Accurate protein structure prediction facilitates drug design, as extensive knowledge about the atomic structure of a given protein enables scientists to target that protein in the human body. However, protein structure identification in certain types of protein images remains challenging, with medium resolution cryogenic electron microscopy (cryo-EM) protein density maps particularly difficult to analyze. Recent advancements in computational methods, namely deep learning, have improved protein modeling. To maximize its accuracy, a deep learning model requires copious amounts of up-to-date training data.

This project explores DeepSSETracer, a …

A Comparison Of Nonverbal And Paraverbal Behaviors In Simulated And Virtual Patient Encounters, Sarah Powers, Mark W. Scerbo, Matthew Pacailler, Macy Kisiel, Baillie Hirst, Ginger S. Watson, Lauren Hamel, Fred Kron

Modeling, Simulation and Visualization Student Capstone Conference

The present study assessed whether trainees display similar nonverbal and paraverbal behaviors when interacting with a simulated (SP) and virtual patient (VP). Sixty second slices of time following four interactions were rated for the presence and frequency of three nonverbal and paraverbal behaviors. Results revealed that students exhibited fewer behaviors in the VP interaction, possibly due to differences social inhibition or fidelity between the two formats.

From Policy Promotion To Research Output: Brief Analysis Of Technical Challenges Of Hospital-Led Artificial Intelligence Research, Yu Zhuang, Cheng Zhou

Bulletin of Chinese Academy of Sciences (Chinese Version)

In recent years, artificial intelligence has become a key direction of medical and health-related research and a hot spot of international competition. In order to investigate the current situation and challenges in hospital-led artificial intelligence researched, this study selects 14 national pilot hospitals to promote the high-quality development of public hospitals as samples, adopts a combination of quantitative and qualitative methods, analyzes the research articles related to artificial intelligence published by the sample hospitals in recent years, and analyzes the technical challenges in the hospital-led artificial intelligence research. The results show that although the number of hospital-led artificial intelligence research …

Image Analysis Methodology For Evaluating Coating Uniformity Of Drug-Coated Angioplasty Balloons, Allison Dobson Zieschang

LSU Master's Theses

Percutaneous transluminal balloon angioplasty (PTA) is a surgical procedure to treat peripheral artery disease, but restenosis causes suboptimal outcomes in many patients. Drug-coated angioplasty balloons (DCBs) were developed to reduce the risk of restenosis by delivering anti-proliferative drugs to the lesion. The efficacy of DCBs depends on factors like drug loading and drug release rate, both of which are directly impacted by the coating uniformity. This thesis evaluates the uniformity of DBCs using fluorescence microscopy, scanning electron microscopy (SEM), qualitative analysis, and an image processing algorithm. Uniformity was quantified by three measurements: histogram standard deviation, histogram percentage of coverage, and …

"Challenging The Known." 16th Annual Research Week: Event Proceedings, University Of The Incarnate Word

Excellence Summit (Research Week) Archived Proceedings

Presentations of completed and ongoing research activity conducted by graduate students, undergraduate students, and faculty at University of the Incarnate Word. Includes poster, podium, visual arts, interactive demo, and creative and performing arts presentations. Coordinated and presented by the Office of Research and Graduate Studies.