Engineering Commons^™

Simulation And Optimization Of A Sheathless Size-Based Acoustic Particle Separator, Shivaraman Asoda

USF Tampa Graduate Theses and Dissertations

Standing surface acoustic waves (SSAW) have been widely used for sorting of cells and particles. However, the major challenges faced with the acoustic driven separation process is the need for an optimized setup to achieve effective separation and the range of particles that can be separated. In this thesis, a custom simulation model is studied to investigate and optimize the separation of varying size particles in a sheathless acoustic separation platform that was developed in our research lab. Specifically, the effect of flowrate, pressure amplitude, wavelength and interdigitated transducers (IDTs) physical parameters on the separation efficiency is explored. We also …

Turbulent Mixers For Protein Folding Experiments, Venkatesh Inguva

Doctoral Dissertations

Protein folding studies require the development of micro-mixers that require less sample, mix at faster rates, and still provide a high signal to noise ratio. Chaotic to marginally turbulent micro-mixers are promising candidates for this application. In this study, various turbulence and unsteadiness generation concepts are explored that avoid cavitation. The mixing enhancements include flow turning regions, flow splitters, and vortex shedding. The relative effectiveness of these different approaches for rapid micro-mixing is discussed. Simulations found that flow turning regions provided the best mixing profile. Various turbulence models are simulated to determine appropriate model of the design requirements. Experimental validation …

Microfluidic Biosensor With Functionalized Gold Nano Particles On Interdigitated Electrodes, Bharath Babu Nunna

Dissertations

The integration of the microfluidics to the biosensor has growing demand with favorable conditions such as reduced processing time and low reagent consumption. The immuno biosensing with the microfluidic platform helped to make the electrochemical biosensing assays portable due to which this sensing mechanism can be easily implemented in point of care devices. The implementation of the biosensing in the microchannels significantly reduces the sample requirement form milli liter (mL) to micro liter (uL), and thus leads to low volume sample requirement during the sensing. The primary factors contributing to the microfluidic biosensors performance are probe immobilization, specific binding and …

Development Of Microdialysis Probes In Series Approach Toward Eliminating Microdialysis Sampling Calibration: Miniaturization Into A Pdms Microfluidic Device, Randy Espinal Cabrera

Graduate Theses and Dissertations

A new microdialysis sampling method and microfluidic device were developed in vitro. The method consisted of using up to four microdialysis sampling probes connected in series to evaluate the relative recovery (RR) of different model solutes methyl orange, fluorescein isothiocyanate (FITC)-dextran average mol. wt. 4,000 (FITC-4), FITC-10, FITC-20, and FITC-40. Different flow rates (0.8, 1.0, and 1.5 µL/min) were used to compare experimentally observed relative recoveries with theoretical estimations. With increasing the number of probes in series, the relative recovery increases and ~100% (99.7% ± 0.9%) relative recovery for methyl orange was obtained. For larger molecules such as fluorescein isothiocyanate …

Electric-Field Assisted Manipulation And Self-Assembly Of Particle Suspensions, Edison Chijioke Amah

Dissertations

The aim of this dissertation is to model the processes by which particles suspended in liquids and at liquid surfaces self-assemble when they are subjected to uniform and non-uniform electric fields. To understand the role of electric forces, three related problems were studied numerically and experimentally.

In the first problem, particles are assumed to be suspended inside a liquid and a nonuniform electric field is applied using electrodes mounted in the domain walls which causes positively polarized particles to collect in the regions where the electric field intensity is locally maximal and the negatively polarized particles collect in the regions …

Microfluidic Technology And Application In Urinal Analysis, Jiwen Xiang

LSU Doctoral Dissertations

Microfluidic technology offers numerous advantages in minimizing and integrating the traditional assays. However, the lack of efficient control components of the microfluidic systems has been hindering the widely commercialization of the technology. The research work in this dissertation focused on the development of effective control components for microfluidic applications.

A linear peristaltic pump was firstly designed, fabricated, and tested for conventional microfluidics by synchronously compressing the microfluidic channel with a miniature cam-follower system in Chapter 2. The miniature cam-follower system and microfluidic chip was prototyped using three-dimensional (3D) printing technology and soft lithography technology. Results from experimental test showed that …

Numerical Modeling Of Capillary-Driven Flow In Open Microchannels: An Implication Of Optimized Wicking Fabric Design, Mehrad Gholizadeh Ansari

Masters Theses

"The use of microfluidics to transfer fluids without applying any exterior energy source is a promising technology in different fields of science and engineering due to their compactness, simplicity and cost-effective design. In geotechnical engineering, to increase the soil's strength, hydrophilic wicking fibers as type of microfluidics have been employed to transport and drain water out of soil spontaneously by taking advantage of natural capillary force without using any pumps or other auxiliary devices. The objective of this study is to understand the scientific mechanisms of the capability for wicking fiber to drain both gravity and capillary water out of …

Developing A 3d In Vitro Model By Microfluidics, Hung-Ta Chien

Dissertations and Theses

In vitro tissue models play an important role in providing a platform that mimics the realistic tissue microenvironment for stimulating and characterizing the cellular behavior. In particular, the hydrogel-based 3D in vitro models allow the cells to grow and interact with their surroundings in all directions, thus better mimicking in vivo than their 2D counterparts. The objective of this thesis is to establish a 3D in vitro model that mimics the anatomical and functional complexity of the realistic cancer microenvironment for conveniently studying the transport coupling in porous tissue structures. We pack uniform-sized PEGDA-GelMA microgels in a microfluidic chip to …

Manufacturing Microfluidic Flow Focusing Devices For Stimuli Responsive Alginate Microsphere Generation And Cell Encapsulation, Michael A. Karasinski

Graduate College Dissertations and Theses

In this paper a novel stimuli responsive hydrogel material, methacrylated sodium alginate beta-cyclodextrin (Alg-MA-β-CD), was used in combination with a microfluidic device to create microspheres. Currently there is no reliable method for fabricating homogeneous stimuli-responsive microspheres, in-house microfluidic devices are not reliable in manufacture quality or long-term use. Alginate hydrogels have many attractive characteristics for bioengineering applications and are commonly used to mimic the features and properties of the extracellular matrix (ECM). Human mesenchymal stem cells (hMSCs) are of top interest to tissue engineers. hMSCs are widely available and can be harvested and cultured directly out of human bone marrow. …

Precise Flow-Control Using Photo-Actuated Hydrogel Valves And Pid-Controlled Led Actuation, Colm Delaney, Simon Coleman, Jeff Whyte, Nigel Kent, Dermot Diamond

Articles

Herein we demonstrate remarkable control of flow within fluidic channels using photo-actuated hydrogel valves. By polymerizing the valves in situ it has been possible to create highly reproducible valves. Through the use of an LED platform and a PID algorithm we have generated extremely accurate flow control and created prototype devices to document their potential application within the microfluidics field.

Improving Sensitivity Of Electrochemical Sensors With Convective Transport In Free-Standing, Carbon Nanotube Structures, Benjamin J. Brownlee, Kevin M. Marr, Jonathan C. Claussen, Brian D. Iverson

Faculty Publications

High-aspect-ratio, porous membrane of vertically-aligned carbon nanotubes (CNTs) were developed through a templated microfabrication approach for electrochemical sensing. Nanostructured platinum (Pt) catalyst was deposited onto the CNTs with a facile, electroless deposition method, resulting in a Pt-nanowire-coated, CNT sensor (PN-CNT). Convection mass transfer enhancement was shown to improve PN-CNT sensor performance in the non-enzymatic, amperometric sensing of hydrogen peroxide (H₂O₂). In particular, convective enhancement was achieved through the use of high surface area to fluid volume structures and concentration boundary layer confinement in a channel. Stir speed and sensor orientation especially influenced the measured current in …

Concurrent Detection And Isolation Of Cellular And Molecular Biomarkers, Wanfeng Huang

Open Access Dissertations

Detection of cancer markers such as protein biomolecules and cancer cells in bodily fluids is of great importance in early diagnosis, prognosis as well as evaluation of therapy efficacy. Numerous devices have been developed for detecting either cellular or molecular targets, however there has not yet been a system that can simultaneously detect both cellular and molecular targets effectively. Molecule and cell-based assays are important because each type of target can tell a different story about the state of the disease and the two types of information can potentially be combined and/or compared for more accurate biological or clinical assessments. …

Fundamental Studies Of Electrochemical Reactions And Microfluidics In Proton Exchange Membrane Electrolyzer Cells, Jingke Mo

Doctoral Dissertations

In electrochemical energy devices, including fuel cells, electrolyzers and batteries, the electrochemical reactions occur only on triple phase boundaries (TPBs). The boundaries provide the conductors for electros and protons, the catalysts for electrochemical reactions and the effective pathways for transport of reactants and products. The interfaces have a critical impact on the overall performance and cost of the devices in which they are incorporated, and therefore could be a key feature to optimize in order to turn a prototype into a commercially viable product. For electrolysis of water, proton exchange membrane electrolyzer cells (PEMECs) have several advantages compared to other …

Movement And Distribution Of Bacteria Near Surfaces, Daniel A. Quinkert, Adib Ahmadzadegan, Arezoo M. Ardekani

The Summer Undergraduate Research Fellowship (SURF) Symposium

Bacteria are found everywhere in nature, including within human/animal bodies, biomedical devices, industrial equipment, oceans and lakes. They can be found in planktonic state within a bulk liquid phase or attached to surfaces with the potential to form biofilms. In this study we are interested in the movement and distribution of bacteria near surfaces. The concentrations and fluid interactions of bacteria were characterized at various distances from a surface. Psuedomonas putida F1 was observed in a suspension near a surface. Bacteria movements were visualized with an inverted microscope at 40x magnification. P. putida F1 exhibited greater density in close proximity …

Pore Scale Transport Of Miscible And Immiscible Fluids In Porous Media, Tolulope O. Odimayomi, Arezoo M. Ardekani

The Summer Undergraduate Research Fellowship (SURF) Symposium

The separation of harmful or valuable substances entrapped in porous media has applications in processes such as enhanced oil recovery, diffusion in tissue, and aquifer remediation. In this study the motion and removal rate of immiscible and miscible solutions have been analyzed to gain understanding of solvent effectiveness as it is diluted due to diffusion or mixing within porous materials. The extraction of oil using water, a surfactant solution of 4% CTAB in water, and a foam produced form the surfactant solution is observed using two dimensional flows between parallel slides containing cylindrical obstacles. The fluid motion is visualized. The …

Analysis Of Shock-Plugs In Quasi-One-Dimensional Compressible Flow, Matthew Alexander Thompson

Graduate Theses - Mechanical Engineering

At small length scales, such as in micro-nozzles, the assumption that a shock wave is infinitesimally thin breaks-down due to the thickness of the shock being non-negligible compared to the dimensions of the nozzle. In this thesis, shock waves of finite thickness, or “shock-plugs,” are modeled using the same methods and assumptions as a standard shock wave analysis. Due to the finite thickness of shock-plugs, however, two additional parameters are required in order to account for the differing inlet and exit areas, as well as the pressure on the side walls of the channel. A “typical” micro-nozzle with appropriate dimensions …

Leidenfrost Energy Barriers, James Peck, Anton Hassebrook, Craig Zuhlke, Troy P. Anderson, Dennis R. Alexander, George Gogos, Sidy Ndao

UCARE Research Products

In this work, an “Energy Barrier” was created to control the motion of Leidenfrost droplets. This barrier was created by functionalizing a portion of a mirror-polished stainless steel 304 surface with Femtosecond Laser Surface Processing (FLSP). FLSP results in superhydrophilic, hierarchical, micro- and nanostructures which are highly wetting and thus have an increased Leidenfrost temperature. Water droplets in the film boiling state were deposited, and propelled by gravity towards the Energy Barrier interface. Room temperature droplets were deposited over a range of surface temperatures beginning with the Leidenfrost temperature of mirror-polished stainless steel 304 and culminating at the Leidenfrost temperature …

Analysis Of Capillary Flow In Interior Corners : Perturbed Power Law Similarity Solutions, Joshua Thomas Mccraney

Dissertations and Theses

The design of fluid management systems requires accurate models for fluid transport. In the low gravity environment of space, gravity no longer dominates fluid displacement; instead capillary forces often govern flow. This thesis considers the redistribution of fluid along an interior corner. Following a rapid reduction of gravity, fluid advances along the corner measured by the column length z = L(t), which is governed by a nonlinear partial differential equation with dynamical boundary conditions. Three flow types are examined: capillary rise, spreading drop, and tapered corner. The spreading drop regime is shown to exhibit column length growth L ~ …

Design, Fabrication, And Testing Of A 3d Printer Based Microfluidic System, Carlton A. Mcmullen

Graduate Theses and Dissertations

A pneumatically actuated PDMS based microfluidic devices were designed and fabricated by soft-lithography. Two types of molds were fabricated out of different material for this experiment. The first mold, (device 1), was fabricated from a sheet of Polymethyl methacrylate (PMMA) material, similar to Plexiglas. The device features were micro-engraved onto the face of the material. The second mold, (device 2), was fabricated from the use of fused deposition modeling (FDM) 3D printing. The pumping efficiency of the PDMS devices was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Tested …

Numerical Studies Of Liquid-Liquid Segmented Flows In Square Microchannels Using A Front-Tracking Algorithm, Eamonn Daire Walker

LSU Doctoral Dissertations

Liquid-liquid segmented flows in a square microchannel are investigated numerically using a hybrid front-tracking/front-capturing method. The code is found to be well-adapted to a large range of flow parameters, but droplet flows are limited by poor accuracy at Laplace number above 100 – 1000 and plug flows are limited by the code’s current inability to adequately model the flow in thin films at low capillary numbers. A Schwarz-Aitken acceleration technique is investigated as a means to reduce computation time, but is found not to be advantageous compared to the parallel multigrid formulation of the code. Numerical simulations are divided into …

Effect Of Junction Geometry On Monodispersed Microdroplet Generation In Microfluidic Aqueous Two-Phase Systems, Young Gyu Nam

Theses and Dissertations

Aqueous two-phase system (ATPS) consists of two immiscible water-based solutions of polymers, which can form phase partitioning. Dextran and polyethylene glycol I used in this thesis is the one of common components of aqueous two-phase system give a reliable and incompatible environment for purification of biomedical products and cellular macromolecules. Recently, ATPS have received increasing attention as a separation method in microfluidic device due to the advantages of biocompatibility, unlimited combination, and low interfacial tension. Hence, it became an important to discover researches related to ATPS microfluidic device.

Microdroplets produced in microfluidic device are a largely interesting phenomenon for various …

Electrokinetic Particle Manipulations In Spiral Microchannels, John Dubose

All Theses

Recent developments in the field of microfluidics have created a multitude of new useful techniques for practical particle and cellular assays. Among them is the use of dielectrophoretic forces in 'lab-on-a-chip' devices. This sub-domain of electrokinetic flow is particularly popular due to its advantages in simplicity and versatility. This thesis makes use of dielectrophoretic particle manipulations in three distinct spiral microchannels. In the first of these experiments, we demonstrate the utility of a novel single-spiral curved microchannel with a single inlet reservoir and a single outlet reservoir for the continuous focusing and filtration of particles. The insulator-based negative-dielectrophoretic (repulsive) force …

Development Of Confocal Imaging Techniques For Probing Interfacial Dynamics In Microscale, Gas-Liquid, Two-Phase Flow, Joseph E. Hernandez

Dissertations, Master's Theses and Master's Reports - Open

Micro-scale, two-phase flow is found in a variety of devices such as Lab-on-a-chip, bio-chips, micro-heat exchangers, and fuel cells. Knowledge of the fluid behavior near the dynamic gas-liquid interface is required for developing accurate predictive models. Light is distorted near a curved gas-liquid interface preventing accurate measurement of interfacial shape and internal liquid velocities. This research focused on the development of experimental methods designed to isolate and probe dynamic liquid films and measure velocity fields near a moving gas-liquid interface. A high-speed, reflectance, swept-field confocal (RSFC) imaging system was developed for imaging near curved surfaces.

Experimental studies of dynamic gas-liquid …

A Parametric Investigation Of A Novel, Modular, Gasketless, Microfluidic Interconnect Using Parallel Superhydrophobic Surfaces, Christopher Ramsey Brown

LSU Master's Theses

The gasketless microfluidic interconnect has the potential to offer a standardized approach to interconnects between modular microfluidic components. This strategy uses parallel superhydrophobic surfaces (contact angle ≥ 150ᴼ) to passively seal adjacent, concentric, microfluidic ports separated by an air gap using a liquid bridge created between the chips. The parallel superhydrophobic surfaces do not require the addition of a gasket or other additional components so that the assembly process scales favorably with an increasing number of fluidic interconnects. The gasketless seal does not contribute to geometric constraint between the component chips which allows alignment between chips to scale favorably with …

There Can Be Turbulence In Microfluidics At Low Reynolds Number, Guiren Wang, F. Yang, Wei Zhao

Faculty Publications

Turbulence is commonly viewed as a type of macroflow, where the Reynolds number (Re) has to be sufficiently high. In microfluidics, when Re is below or on the order of 1 and fast mixing is required, so far only chaotic flow has been reported to enhance mixing based on previous publications since turbulence is believed not to be possible to generate in such a low Re microflow. There is even a lack of velocimeter that can measure turbulence in microchannels. In this work, we report a direct observation of the existence of turbulence in microfluidics with Re on the order …

Multiphase Flows With Digital And Traditional Microfluidics, Michael Andrew Nilsson

Open Access Dissertations

Multi-phase fluid systems are an important concept in fluid mechanics, seen every day in how fluids interact with solids, gases, and other fluids in many industrial, medical, agricultural, and other regimes. In this thesis, the development of a two-dimensional digital microfluidic device is presented, followed by the development of a two-phase microfluidic diagnostic tool designed to simulate sandstone geometries in oil reservoirs. In both instances, it is possible to take advantage of the physics involved in multiphase flows to affect positive outcomes in both.

In order to make an effective droplet-based digital microfluidic device, one must be able to precisely …

Development Of A Pdms Based Micro Total Analysis System For Rapid Biomolecule Detection, Balaji Srinivasan Venkatesh

Graduate Theses and Dissertations

The emerging field of micro total analysis system powered by microfluidics is expected to revolutionize miniaturization and automation for point-of-care-testing systems which require quick, efficient and reproducible results. In the present study, a PDMS based micro total analysis system has been developed for rapid, multi-purpose, impedance based detection of biomolecules. The major components of the micro total analysis system include a micropump, micromixer, magnetic separator and interdigitated electrodes for impedance detection. Three designs of pneumatically actuated PDMS based micropumps were fabricated and tested. Based on the performance test results, one of the micropumps was selected for integration. The experimental results …

Magnetic Manipulation Of Particles And Cells In Ferrofluid Flow Through Straight Microchannels Using Two Magnets, Jian Zeng

All Theses

Microfluidic devices have been increasingly used in the past two decades for particle and cell manipulations in many chemical and biomedical applications. A variety of force fields have been demonstrated to control particle and cell transport in these devices including electric, magnetic, acoustic, and optical forces etc. Among these particle handling techniques, the magnetic approach provides clear advantages over others such as low cost, noninvasive, and free of fluid heating issues. However, the current knowledge of magnetic control of particle transport is still very limited, especially lacking is the handling of diamagnetic particle. This thesis is focused on the magnetic …

Electrohydrodynamic Manipulation Of Liquid Droplet Emulsions In A Microfluidic Channel, Jonathan Wehking

Electronic Theses and Dissertations

This work specifically aims to provide a fundamental framework, with some experimental validation, for understanding droplet emulsion dynamics in a microfluidic channel with an applied electric field. Electrification of fluids can result in several different modes of electrohydrodynamics (EHD). Several studies to date have provided theoretical, experimental, and numerical results for stationary droplet deformations and some flowing droplet configurations, but none have reported a method by which droplets of different diameters can be separated, binned and routed through the use of electric fields. It is therefore the goal of this work to fill that void and report a comprehensive understanding …

Electrokinetic Mixing And Separation In Microfluidic Systems, Fang Yang

Theses and Dissertations

Electrokinetics involves the study of liquid or particle motion under the action of an electric field; it includes electroosmosis, electrophoresis, dielectrophoresis, and electrowetting, etc. The applications of electrokinetics in the development of microfluidic devices have been widely attractive in the past decade. Electrokinetic devices generally require no external mechanical moving parts and can be made portable by replacing the power supply by small battery. Therefore, electrokinetic based microfluidic systems can serve as a viable tool in creating a lab-on-a-chip (LOC) for use in biological and chemical assays. Here we present our works of electrokenitic based mixing and separation in microfluidics …