Engineering Commons^™

Fluorescent Imaging On A Microfluidics Chip For Quantification Of Leukocyte Count, Aneeka Majid

Biomedical Engineering Undergraduate Honors Theses

We have demonstrated a method for conducting a leukocyte count in whole blood using a microfluidics chip and epi-fluorescence setup. Leukocyte counts provide physicians with a wealth of information about a patient’s medical condition and as such are routinely completed for many hospital visits. Miniaturization of this diagnostic tool may enable physicians to provide healthcare in resource-limited settings, where patients would otherwise not receive this test. The microfluidics chip was fabricated in polydimethylsiloxane (PDMS) using soft-film lithography. Following further processing and cleaning, the PDMS mold is exposed to UV-ozone for surface activation, and then sealed with a glass coverslip to …

Development Of An In Vitro Myogenesis Assay, Anna Arnaud

Biomedical Engineering Undergraduate Honors Theses

The objective of this study was to explore the interaction between mouse C2C12 cells and the extracellular matrix, particularly the process of myoblasts converting to myocytes. This study aimed to create a myogenesis assay that presents a process to effectively monitor the development of mouse C2C12 myoblasts into differentiated skeletal myotubes through detection of the protein MyoD. Myogenesis, the development of muscle tissue, occurs when muscle progenitor cells, myoblasts, fuse to form multinucleated myotubes, followed by cell fusion and resulting in a myofiber capable of contraction. An in vitro myogenesis assay would enable further research to efficiently test the effect …

Multiplexed Microfluidic Loop-Mediated Isothermal Amplification Of The 16s Rrna Gene For The Diagnosis Of Neonatal Sepsis In Resource-Limited Environments, Griffin Thomas Sonaty

Biomedical Engineering Undergraduate Honors Theses

Bacterial infection, or sepsis, places a disproportionately high burden on newborns in developing countries. This is due in part to a lack of diagnostic tools suitable for sustainable use in resource-limited nurseries. One potential vehicle for a new diagnostic assay is loop-mediated isothermal amplification (LAMP), a high-yield DNA amplification method. LAMP has been used to detect single genes from bacteria in blood serum samples to aid in sepsis diagnosis. While specific, this approach can only provide detection for one species at a time. LAMP could be adapted to detect a broader set of bacteria, while retaining a degree of specificity …

Intracellular Uptake Of Chitosan Nanoparticle-Based Vaccines, Katherine E. Wilson

Biomedical Engineering Undergraduate Honors Theses

Particle-based delivery systems are a promising approach to enhance antigen-specific immunity for use in vaccines. Chitosan, a natural polysaccharide derived from the exoskeletons of crustaceans, shows great potential as a vaccine carrier due to its favorable properties as a biomaterial, including biocompatibility and biodegradability. In this study, the intracellular uptake of chitosan nanoparticles is explored and compared to that of other commonly used particle-based delivery vehicles, such as poly (lactic-co-glycolic acid) (PLGA). Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) was used as a model protein antigen and encapsulated in both chitosan particles via precipitation-coacervation and PLGA particles via double emulsion solvent …

The Production, Properties And Applications Of The Zinc Imidazolate, Zif-8., John L. Tatarko Jr.

Electronic Theses and Dissertations

Venna, Carreon, and Jasinski produced and characterized the first samples of the zinc imidazolate framework ZIF-8 at the University of Louisville in 2010. In this dissertation the production, properties, and applications of this unique metal-organic framework are explored. Previously, only minute laboratory amounts (1/4 gram), of ZIF-8 were produced via time-consuming and expensive processes. Production quantities have been synthesized via both a continuous and a batch process using a spray drying operation to effect separation of the solid product (ZIF-8) from the mother liquor. Approximately 85% of the mother liquor (methanol), can be recovered from the spray dryer resulting in …

Zinc Oxide Based Inks For Semiconductor Applications, Shreyas Pathak

Masters Theses

Conventional printing methods are gaining popularity in the manufacturing of electronic components hence being termed as Printed Electronics (PE). IDTechEx has predicted that the total market size of flexible electronics by 2024 would be $70.4 billion. Their report predicts that majority of applications would consume conductive inks. (1)

The main challenge in PE is developing suitable inks for the different printing processes being used to manufacture these electronic components. The experiments in this work mainly involved selecting suitable components for formulating solvent based, semiconductive ZnO inks and then sintering using the NovaCentrix® PulseForge® 1200. ZnO inks were also doped with …

Feasibility Testing Of Chill Filtration Of Brown Spirits To Increase Product Stability., Juan Cristobal Merizalde Carrillo

Electronic Theses and Dissertations

This thesis summarizes a feasibility study focused on filtering American whiskey, matured in new toasted and charred casks, at 25 ºF in order to remove haze which forms when product is diluted from cask strength (62% ABV) to bottling strength (40 – 50% ABV). Chill filtration is a process already implemented in the brown spirits industry in order to guarantee the stability of products at different ethyl alcohol concentrations. The filtration trials and cellulose-pad performance were evaluated in terms of economics, turbidity reduction, test time, throughput, color reduction, and shelf life stability. Those trials with satisfactory overall color and stability …

Characterization Of Novel Chitosan/Polyelectrolyte Nanoparticles, Michaela Wynne Mertz

Biomedical Engineering Undergraduate Honors Theses

Chitosan-based nanoparticles are under investigation as delivery vehicles for drugs, genes, and proteins. Due to the biocompatibility, mucoadhesive nature, and biodegradable nature of chitosan and binding qualities, anionic nature, and biocompatible qualities of heparin, chitosan/heparin nanoparticles (CHNPs) are a promising platform for the delivery of labile proteins. However, the literature lacks a definitive process to control the size, efficiency, and yield of CHNPs at higher concentrations of reagents. Additionally there is a need to improve loading efficiency of CHNPs. In this study CHNPs have been synthesized and characterized for size, yield, protein loading efficiency, and release. The CHNPs were prepared …

Fractionation Of Chitosan Using A Novel Precipitation Method, Davis J. Ward

Biomedical Engineering Undergraduate Honors Theses

Chitosan is a derivative of chitin, a biopolymer found in the exoskeletons of crustaceans. Chitosan is under heavy investigation in the field of drug delivery due to its wide versatility. One such delivery mechanism is the use of hydrogels in cancer immunotherapy delivery. The viscosity of chitosan in its use as a hydrogel depends on the length of chitosan’s N-acetyl D-glucosamine chains, which in turn determines the molecular weight of chitosan. Because there is such a wide range of viscosity in a typical sample of chitosan, there is a need for a simple technique to fractionate the chitosan based on …

Monitoring The Effects Of Mmp Inhibitors On Extracellular Matrix Degradation For Use In Implant Protection, Jake D. Jones

Biomedical Engineering Undergraduate Honors Theses

In order to lengthen the functional time of implantable ECM, the mechanism of degradation must be inhibited. The matrix metalloproteases are the primary method by which ECM is broken down, and the TIMP family of proteins exists as proof that inhibition of these proteases is possible naturally. In particular, the glycoprotein TIMP-1 plays an inhibitory role with most of the MMPs including the collagenases. Furthermore, there exist synthetic or chemical molecules that likewise inhibit the catalytic actions of MMPs. One such molecule is Batimastat (BB-94) which has been proven to be a dose dependent inhibitor of MMPs during the inflammatory …

Characterization And Development Of Tsi Three-Dimensional Laser Doppler Velocimetry System, John Andrew Dominick

Chemical Engineering Undergraduate Honors Theses

The study of dense gas dispersion at atmospheric conditions is critical to understand the safety concerns of industrial operations where large amounts of toxic or flammable materials are stored and generated. A large airborne release of these materials into the atmosphere could have catastrophic effects. The Chemical Hazards Research Center (CHRC) at the University of Arkansas performs important research on dense gas dispersion. The CHRC houses the world’s largest ultra-low speed wind tunnel, which is used to simulate dispersion at atmospheric conditions. Substantial amounts of data, including velocities, are recorded for modeling real life gas dispersion events. Before conducting experiments, …

Separation Of Emulsified Oil From A Brackish Water Stream Using Ultrafiltration And Coalescence, Casey W. Polk

Chemical Engineering Undergraduate Honors Theses

In the past 40 years, a variety of enhanced oil recovery (EOR) methods have been developed and applied to mature, mostly depleted, and shale formation oil reservoirs. Chemical and sonic stimulation are two enhanced oil recovery methods in which emulsions are created either as a primary or secondary effect. The resulting viscosity of the oil in water emulsion is considerably lower than that of dry crude, thus increasing recovery from pay zones. During chemical enhanced oil recovery, caustic or surfactants are injected into oil reservoirs, which results in the creation of stable oil-water emulsions. The emulsions from chemical enhanced oil …

Effect Of Sodium Acetate On Butanol Production By Clostridium Saccharoperbutylacetonicum Via Abe Fermentation, Brigetta Super

Chemical Engineering Undergraduate Honors Theses

Clostridium saccharoperbutylacetonicum is used in ABE fermentation which historically produces a solvent mixture of 3:6:1 parts acetone, butanol, and ethanol respectively. The fermentation process undergoes two phases, an acidogenesis phase which produces butyric acid, and a solventogenic phase which reutilizes the butyric acid, making butanol. For ABE fermentation to compete economically with butanol production from petroleum, the concentration of butanol in the solvent product must be increased dramatically. Unpublished research by Carver student Kayla Kimbrough in the summer of 2012 showed that the addition of salts (sodium acetate) to PYG fermentation media increased butanol production from Clostridium saccharoperbutylacetonicum significantly. When …

Electrocoagulation For Sulfate Removal, Michael A. Layton

Chemical Engineering Undergraduate Honors Theses

In the United States, one of the major rising issues is water shortage, especially in Western, inland states with arid climates. Not only do deficiencies exist for potable water, but for irrigation and agricultural purposes as well. Currently, the Brackish Groundwater National Desalination Research Facility in Alamogordo, New Mexico has four separate wells that are being used for experimentation. A major concern is that a considerable amount of this water is currently wasted by being sent to evaporation ponds without any sort of treatment for human consumption. The WERC-A-HOLICS have identified two possible methods to alleviate this problem. Electrocoagulation (EC) …

Electrocoagulation For Sulfate Removal, Hailey Marie Dunsworth

Chemical Engineering Undergraduate Honors Theses

In the United States, one of the major rising issues is water shortage, especially in Western, inland states with arid climates. Not only do deficiencies exist for potable water, but for irrigation and agricultural purposes as well. Currently, the Brackish Groundwater National Desalination Research Facility in Alamogordo, New Mexico has four separate wells that are being used for experimentation. A major concern is that a considerable amount of this water can be used as another reliable source for potable water if treated. The WERC-A-HOLICS have identified two possible methods to alleviate this problem. Electrocoagulation (EC) is a technology useful for …

Radiative Cooling To The Night Sky, Alexander R. Enderlin

Chemical Engineering Undergraduate Honors Theses

Silicon wafer fabrication facilities require a significant amount of temperate cooling water to meet high internal cooling loads. The tertiary chilled water systems operate year round to fulfil the constant cooling load needed for production. Conventional chillers require a large amount of electricity to cool the water by the vapor compression cycle. As energy costs increase and environmental stewardship becomes the norm in industry, sustainable technologies are needed to chill process cooling water without the significant energy consumption of today’s chillers. Night sky radiation cools a medium by radiating heat from a warm medium to the colder night sky. The …

Impregnation And Adsorption Of Rare Earth Elements On Amberlite Xad-7, Bobby G. Hampton

Chemical Engineering Undergraduate Honors Theses

Beads were washed then impregnated with an organophosphorous extractant D2EHPA. The washing process, as well as factors in the impregnation process were studied. Water was deemed sufficient to prewash the beads before use over small and large amounts of acid with acetone. The impregnation of beads at an Amberlite D2EHPA ratio of 1:1 was determined to be best. Absorption isotherms of Neodymium were the first to be studied. By testing different concentrations, Amberlite was determined to absorb the max amount at 4000 ppm. The pH values from 2-7 were determined to have no effect on the amount of Neodymium absorbed …

Separatome Of Escherichia Coli Analysis Of Deae Chromatography As Ph Of Loading Changes, Rita E. Tejada Vaprio

Chemical Engineering Undergraduate Honors Theses

Recombinant DNA technology is used to produce therapeutics in host organisms and undergoes several purification steps before it can be used by the end user. Escherichia coli which has been widely studied has been the microorganism of choice mainly because of its low production costs. The primary objective of this study was to investigate the optimal conditions to purify a protein of interest with antifungal properties. Varying the loading pH alters the overall charge of both the target protein and host cell proteins (HCPs). Identifying genomic proteins that remain bound regardless of loading pH on DEAE columns will guide future …

Electrocoagulation For Sulfate Removal, Gina Marie Densmore

Chemical Engineering Undergraduate Honors Theses

In the United States, one of the major rising issues is water shortage, especially in Western, inland states with arid climates. Not only do deficiencies exist for potable water, but for irrigation and agricultural purposes as well. Currently, the Brackish Groundwater National Desalination Research Facility in Alamogordo, New Mexico has four separate wells that are being used for experimentation. A major concern is that a considerable amount of this water is currently wasted by being sent to evaporation ponds without any sort of treatment for human consumption. The WERC-A-HOLICS have identified two possible methods to alleviate this problem. Electrocoagulation (EC) …

Use Of Peptoid Microspheres To Improve Enzyme Linked Immunosorbent Assay (Elisa) Microarray For Early Cancer Detection, Valerie Del Carmen Reyez Loayza

Chemical Engineering Undergraduate Honors Theses

The pursuit of an efficient and economical technique for early detection of cancer has led to the development of sophisticated biosensors for antigen analysis. As biosensors become more complex, coatings are necessary and important. The preferred coatings need to have the following properties: high surface area for binding, resistance to biofouling, and flexible synthesis. The focus of this project is to determine whether using peptoid microsphere-coated glass slides in the enzyme linked immunosorbent assay (ELISA) microarray technique is better than using commercially available glass slides. Current microarray slides are coated with reactive groups, such as amines, epoxides, or aldehydes. It …

Ultrasonic Hoguanauts Werc Task 2: Separation Of Oil From Water, Jesus Eduardo Martinez

Chemical Engineering Undergraduate Honors Theses

Engineering, Chemical Engineering, Environmental

Investigating Polymer Based Scaffolds For Urinary Bladder Tissue Engineering, Srikanth Sivaraman

All Dissertations

Current surgical treatments for urinary bladder disorders rely on the use of autologous intestinal segments and xenografts such as small intestinal submucosa, which suffer from various complications including mechanical mismatch and graft shrinkage. Despite early promises of bladder tissue engineering, a recent report of unsuccessful clinical trials suggests that the technology needs further improvement and evaluation through animal models of bladder dysfunction. Therefore, the objective of this doctoral dissertation was to characterize a viable bladder tissue scaffold (patch) which mimics the mechanical properties of the bladder, maintains the phenotype of the BSMC seeded in it and finally, tested in vivo …

Thermal Invisibility Based On Scattering Cancellation And Mantle Cloaking, M Farhat, P.-Y. Chen, H Bagci, C. Amra, S Guenneau, A Alù

Electrical and Computer Engineering Faculty Research Publications

We theoretically and numerically analyze thermal invisibility based on the concept of scattering cancellation and mantle cloaking. We show that a small object can be made completely invisible to heat diffusion waves, by tailoring the heat conductivity of the spherical shell enclosing the object. This means that the thermal scattering from the object is suppressed, and the heat flow outside the object and the cloak made of these spherical shells behaves as if the object is not present. Thermal invisibility may open new vistas in hiding hot spots in infrared thermography, military furtivity, and electronics heating reduction.

Recent Research Progress For Non-Pt-Based Oxygen Reduction Reaction Electrocatalysts In Fuel Cell, Ping Song, Ming-Bo Ruan, Jing Liu, Guang-Jun Ran, Wei-Lin Xu

Journal of Electrochemistry

The expensive Pt-based catalyst suffers from its susceptibility to time-dependent drift, methanol crossover, and carbon monoxide (CO) deactivation, which will block the large-scale commercial application of fuel cells. It is, therefore, necessary to develop efficient, low-cost, highly stable non-precious metal oxygen reduction reaction (ORR) catalysts with high catalytic performance. Here, we will pay attention to the research progress of non-precious metal and heteroatom-doped (N, B, P, S, F) metal-free ORR catalysts. Importantly, we mainly focus on the work developed by Xu’s group with the Fe-based non-precious metal and F/NF-doped metal-free ORR catalysts. The purpose of these works is to improve …

Preparation And Properties Of Binary Oxides CoXCr1-XO3/2 Electrocatalysts For Oxygen Evolution Reaction, Tai-Lai Yang, Wen-Yan Dong, Hui-Min Yang, Li Zhang, Zhen-Hai Liang

Journal of Electrochemistry

Binary metal mixed oxides of Co and Cr with nominal compositional formulas, Co_xCr_1-xO_3/2 (x=0, 0.2, 0.4, 0.6, 0.8 and 1.0), were obtained by thermal decomposition method. The morphologies, crystal forms and valence states of the catalyst powders were analyzed by SEM, XRD and XPS, respectively. And the electrochemical properties, overpotentials and stabilities of Co_xCr_1-xO_3/2 electrodes for oxygen evolution reaction (OER) in an alkaline solution were evaluated by linear sweep voltammetry (LSV), staircase voltammetry and chronoamperometry, respectively. The results indicated that the mixtures of Co₃O₄ and eskolaite-Cr_{2 …}

Synthesis And Electrochemical Properties Of Cnts/V2O5 Hollow Microspheres, Liang Hao, Lai-Fa Shen, Jie Wang, Jia-Jia Zhu, Xiao-Chen Zhao, Xiao-Gang Zhang

Journal of Electrochemistry

The carbon nanotubes/vanadium oxides (CNTs/V₂O₅) hollow microspheres (VOC) were prepared via solvothermal process. The effects of the ratio for CNTs to V₂O₅ on the morphologies, structures and electrochemical performances were systemically investigated. The results indicate that CNTs dramatically enhanced the rate performances of VOC composite electrodes. When the ratio of CNTs is 7.1%, the VOC composite electrode exhibited the best electrochemical performance, which delivered a specific capacitance of 346 F·g^-1 at 0.5 A·g^-1 and maintained 75% at 8 A·g^-1 in 5 mol L^-1 LiNO₃. A hybrid capacitor …

Porous Co3O4 Hollow Nanospheres Cathode Catalyst For High-Capacity And Long-Cycle Li-Air Batteries, Tong Liu, Na Li, Qing-Chao Liu, Xin-Bo Zhang

Journal of Electrochemistry

In this paper, a high specific surface area of porous Co3O4 hollow nanospheres was successfully synthesized via hydrothermal carbonization at 140 °C, followed by calcination using cobalt nitrate hexahydrate (Co(NO3)2·6H2O), hexamethylenetetramine (HMT), sucrose, and sodium citrate (Na3C6H5O7). The porous Co3O4 hollow nanospheres consisted of nanoparticles with high specific surface area of mesoporous structure, and could provide active reaction sites for OER and ORR. When used as lithium-air battery cathode catalyst, the Co3O4/Super P (SP) electrode exhibited excellent cycle performance, resulting in high capacity and long life of lithium-air batteries.

Syntheses And Electrochemical Performances Of Li4Ti5O12 Anode Materials For Lithium Ion Battery, Yong-Long Zhang, Xue-Bu Hu, Yao-Qiong Wang, Dong-Hai Huang

Journal of Electrochemistry

Series of spinel lithium titanate (Li₄Ti₅O₁₂) materials were synthesized by one-step solid-state reaction using lithium acetate and TiO₂ as raw materials under different temperatures (750/800/850 °C) and atmospheres (N₂/air). The as-prepared Li₄Ti₅O₁₂ materials were investigated by thermogravimetric analysis, X-ray diffraction, scanning electron microscopy, cyclic voltammotry and charge-discharge test to analyze their crystal structures, microstructures and electrochemical performances. The results show that the Li₄Ti₅O₁₂（L-800N）material obtained at 800 °C under nitrogen atmosphere exhibited better electrochemical performance and smaller particle size. The initial …

Simulation Of Deformation Of Red Blood Cells, Tai-Hsien Wu

Research and Creative Activities Poster Day

Using simulation to discuss the deformation of cells is not only efficient, but also inexpensive. In this study, a red blood cell (RBC) model is introduced which is simple but rigorous. This RBC model has been stretched to examine its properties and its results agree well with an experiment which was conducted in 2003. Therefore, the model allows researchers to conduct studies related to deformation of RBCs or investigate properties of RBCs. Based on this RBC model, we can couple it with the computational fluid dynamics to investigate the interaction between cells and flows, as well as mimic other cells …

Life-Cycle Energy And Ghg Emissions Of Forest Biomass Harvest And Transport For Biofuel Production In Michigan, Fengli Zhang, Dana M. Johnson, Jinjiang Wang

College of Business Publications

High dependence on imported oil has increased U.S. strategic vulnerability and prompted more research in the area of renewable energy production. Ethanol production from renewable woody biomass, which could be a substitute for gasoline, has seen increased interest. This study analysed energy use and greenhouse gas emission impacts on the forest biomass supply chain activities within the State of Michigan. A life-cycle assessment of harvesting and transportation stages was completed utilizing peer-reviewed literature. Results for forest-delivered ethanol were compared with those for petroleum gasoline using data specific to the U.S. The analysis from a woody biomass feedstock supply perspective uncovered …