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Articles 61 - 90 of 1182
Full-Text Articles in Engineering
Evaluation Of Cellulose Nanocrystal Inks And Their Structural Characteristics For 3d Bioprinting Of Customized Scaffolds, Patrick William Kuczwara
Evaluation Of Cellulose Nanocrystal Inks And Their Structural Characteristics For 3d Bioprinting Of Customized Scaffolds, Patrick William Kuczwara
Graduate Theses and Dissertations
3D bioprinting of biological scaffolds requires control of the physicochemical properties of each unique structures. A promising material for control of properties is hydrogels, which can help create biomimetic scaffolds with controlled spatial arrangement of materials by integrating biological materials directly into layers during the bioprinting process. Nanocellulose offers a unique combination of properties including mechanical, biomimetic, and biocompatibility. These properties offer flexibility over the types, shapes, and applications of their printed hydrogel scaffolds, (i.e., tissue, drug, encapsulation). However, 3D bioprinting of nanocellulose-based hydrogels requires high loading percentages (i.e., >10 wt%) or chemical crosslinkers (i.e., bis(acyl)phosphane oxides (BAPO)). High solid …
Dendrimer-Based Drug Delivery Systems: History, Challenges, And Latest Developments, Juan Wang, Boxuan Li, Li Qiu, Xin Qiao, Hu Yang
Dendrimer-Based Drug Delivery Systems: History, Challenges, And Latest Developments, Juan Wang, Boxuan Li, Li Qiu, Xin Qiao, Hu Yang
Chemical and Biochemical Engineering Faculty Research & Creative Works
Since the first dendrimer was reported in 1978 by Fritz Vögtle, dendrimer research has grown exponentially, from synthesis to application in the past four decades. The distinct structure characteristics of dendrimers include nanoscopic size, multi-functionalized surface, high branching, cavernous interior, and so on, making dendrimers themselves ideal drug delivery vehicles. This mini review article provides a brief overview of dendrimer's history and properties and the latest developments of dendrimers as drug delivery systems. This review focuses on the latest progress in the applications of dendrimers as drug and gene carriers, including 1) active drug release strategies to dissociate drug/gene from …
Evaluating The Viscoelastic Properties Of Alligator Skin Gelatin, Alligel, Compared To Mammalian And Fish Gelatins, Sarah E. Jimenez
Evaluating The Viscoelastic Properties Of Alligator Skin Gelatin, Alligel, Compared To Mammalian And Fish Gelatins, Sarah E. Jimenez
Honors Theses
No abstract provided.
Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine
Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.
Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly interspaced …
Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar
Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Wastewater based epidemiology (WBE) allows for the tracking of nucleic acid of SARS-CoV-2 in wastewater which gives the opportunity for the public and government officials to be informed about the infectivity of the virus in a community. Advances have been made in WBE that have allowed for higher performance, lower resource use, and faster turnaround time. An adapted concentration method of spin column direct extraction has yielded a proxy virus recovery of 83%, consumable cost of $2.01 per sample, and a turnaround time of 0.33 hour/sample. Other concentration methods have lower proxy virus recoveries, comparable cost, and comparable turnaround time. …
Encapsulation Of Antimicrobial Peptides In Bicontinuous Microemulsions For Topical Delivery To Surgical Site Infections And Chronic Wounds, Madison A. Oehler
Encapsulation Of Antimicrobial Peptides In Bicontinuous Microemulsions For Topical Delivery To Surgical Site Infections And Chronic Wounds, Madison A. Oehler
Masters Theses
Surgical site infections and chronic wounds, especially those caused by antibiotic-resistant microorganisms, result in hospitalization and fatalities each year. Methods to prevent these infections, such as cleaning and preparing medical tools, have had minimal success in preventing infections. Further, antibiotic treatments have become less successful in treating infections and wounds because of antibiotic-resistant bacteria. Antimicrobial peptides (AMP) are a possible treatment solution for wound infections. AMPs are oligopeptides that occur in nature or can be synthesized in vitro which possess a broad spectrum of antimicrobial activity against bacteria and other harmful microorganisms. AMPs operate by disrupting the packing arrangements of …
Hypoxic Incubation Chamber, Simone Lisette Helfrich, Makenzie Nicole Jones
Hypoxic Incubation Chamber, Simone Lisette Helfrich, Makenzie Nicole Jones
Biomedical Engineering: Graduate Reports and Projects
This paper describes the design, manufacturing, and testing of a novel controllable hypoxic incubator with fully functional oxygen gas control and temperature control in a humid environment. On the current market, a majority of the few hypoxic incubators use pre-mixed gas that does not offer precise control over gas concentration. The objective for this project was to create a chamber that allows the user to set the O2 concentration to varying set points of % O2 while maintaining the chamber at a constant body temperature, CO2 level, humidity, and sterility. To start the project, multiple concepts were developed for the …
Functionality Of The Gracilis Collateral Is Impaired Following Arteriogenesis And Cell Transplantation, Ada A. Tadeo
Functionality Of The Gracilis Collateral Is Impaired Following Arteriogenesis And Cell Transplantation, Ada A. Tadeo
Biomedical Engineering
Peripheral Arterial Disease involves narrowed arteries, reducing blood flow to limbs. Increasing blood flow to those extremities is possible by redirecting the blood to flow through natural bypasses (i.e. collateral arteries), which can enlarge via arteriogenesis to maintain blood supply once the prominent arteries have become occluded. This study aims to investigate how arteriogenesis affects collateral function, if myoblast transplantation can stimulate collateral growth, and how that in turn may affect collateral function. Femoral artery ligation was performed to mimic the blockage that occurs in patients with ischemic diseases on lean mice and mice with diet induced obesity (DIO). A …
The Influence Of Processing And Additives On Cellulose Nanofiber Properties For Orthopedic Application, Mitchell P. Chesley
The Influence Of Processing And Additives On Cellulose Nanofiber Properties For Orthopedic Application, Mitchell P. Chesley
Electronic Theses and Dissertations
Current orthopedics are separated into three different classes of materials, metals, polymers, and ceramics. While these devices have had success throughout the years they are not without their faults. Metallic devices for example are usually extraordinarily stiff when compared with the surrounding bone. This difference in stiffness induces localized stress-shielding promoting cortical atrophy, which can lead to osteoporosis. Polymers while having the capacity of being biodegradable and bioabsorbable also have the potential to incite localized demineralization and weakness in surrounding bone. A result of breakdown byproducts not efficiently being evacuated from the area, which additionally acts as catalysts expediating the …
Labeling Melanoma Cells With Black Microspheres For Improved Sensitivity In Detection Via Photoacoustic Flow Cytometry, Tori Kocsis
Electronic Theses and Dissertations
Melanoma is an aggressive form of skin cancer known for developing into metastatic disease. Current clinical diagnostics, including medical imaging and tissue biopsy, provide a poor prognosis since the cancer is in the late stages of disease progression. In recent years, photoacoustic flow cytometry has allowed for the detection of circulating melanoma cells within patient blood samples in vitro. Although this method exploits the naturally-produced melanin within the cells, it has only successfully detected highly-pigmented melanoma cell lines. Since various forms of melanoma exist, each with varying melanin concentrations, this research aims to provide a novel method for detecting lightly-pigmented …
Novel 3d Bench Top Model For Vascular Calcification Research, Ursla-Marie K. Offiah
Novel 3d Bench Top Model For Vascular Calcification Research, Ursla-Marie K. Offiah
Theses and Dissertations
Cardiovascular disease is the leading cause of non-communicable disease in the whole world killing 17 million people in 2012. Among the many vascular diseases is vascular calcification (VC) which is the mineral build up in the walls of blood vessels. Medial calcification is the plaque buildup in the medial layer of the blood vesicle that is characterized by arterial stiffness and high blood pressure. Current calcification research involves two dimensional (2D) lab methods such as flat petri dishes to investigate the mechanism that causes and inhibits vascular calcification. Research has shown that the use of three-dimensional (3D) models can be …
Upstream Methods For Enhancing Engineered Curcumin Biosynthesis, Caleb D. Barton
Upstream Methods For Enhancing Engineered Curcumin Biosynthesis, Caleb D. Barton
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Curcumin is a bright orange compound with myriad applications for human health and wellness. Curcumin occurs naturally in the plant Curcuma longa (commonly known as turmeric) but must be extracted from the roots in an environmentally unfriendly fashion to obtain commercially relevant amounts of the compound. In addition, extraction of curcumin from turmeric spice yields a mixture of various curcuminoids, presenting an issue for isolating it in its pure form and complicating its use in clinical settings.
Heterologous biosynthetic production of curcumin in Escherichia coli has been used extensively as a viable alternative to plant extraction but suffers from poor …
Modeling The Impact And Accelerating The Process Of Transitioning To A Sustainable Healthy Diet Through Decision Support Systems, Prince Agyemang
Modeling The Impact And Accelerating The Process Of Transitioning To A Sustainable Healthy Diet Through Decision Support Systems, Prince Agyemang
Graduate Theses and Dissertations
Food production and consumption are essential in human existence, yet they are implicated in the high occurrences of preventable chronic diseases and environmental degradation. Although healthy food may not necessarily be sustainable and vice versa, there is an opportunity to make our food both healthy and sustainable. Attempts have been made to conceptualize how sustainable healthy food may be produced and consumed; however, available data suggest a rise in the prevalence of health-related and negative environmental consequences of our food supply. Thus, the transition from conceptual frameworks to implementing these concepts has not always been effective. This paper explores the …
Examining Metal Contents In Primary And Secondhand Aerosols Released By Electronic Cigarettes, Kashala Fabrice Kapiamba, Weixing Hao, Stephen Adom, Wenyan Liu, Yue-Wern Huang, Yang Wang
Examining Metal Contents In Primary And Secondhand Aerosols Released By Electronic Cigarettes, Kashala Fabrice Kapiamba, Weixing Hao, Stephen Adom, Wenyan Liu, Yue-Wern Huang, Yang Wang
Chemistry Faculty Research & Creative Works
The usage of electronic cigarettes (ECs) has surged since their invention two decades ago. However, to date, the health effects of EC aerosol exposure are still not well understood because of insufficient data on the chemical composition of EC aerosols and the corresponding evidence of health risks upon exposure. Herein, we quantified the metals in primary and secondhand aerosols generated by three brands of ECs. By combining aerosol filter sampling and inductively coupled plasma mass spectrometry (ICP-MS), we assessed the mass of metals as a function of EC flavoring, nicotine concentration, device power, puff duration, and aging of the devices. …
Monitoring Neuronal Activity In Vivo With A Novel Implantable Battery-Free Wireless System, Carolina Moncion
Monitoring Neuronal Activity In Vivo With A Novel Implantable Battery-Free Wireless System, Carolina Moncion
FIU Electronic Theses and Dissertations
Intracranial neuronal recordings are essential for understanding neuronal function. They can be classified into levels like electrocorticography (ECoG)/local field potentials (LFP) and neuronal spiking activity. Typically, wired systems have been used to conduct these recordings; however, they have been linked to drawbacks, including infections, subdural hematomas, and are limited to a clinical setting, all of which can be detrimental to the patients’ quality of life. To address these issues, wireless biotelemetric devices have been increasingly adopted; nevertheless, they are coupled with their own drawbacks like bulky components and heat-generating batteries. Miniaturized battery-free systems have been developed for addressing these concerns, …
Aerodynamics And Turbulent Wake-Flow Characteristics Of Owls During Flapping Flight, Krishnamoorthy Krishnan
Aerodynamics And Turbulent Wake-Flow Characteristics Of Owls During Flapping Flight, Krishnamoorthy Krishnan
Electronic Theses and Dissertations
Owls exhibit unique flight capabilities in the low Reynolds number flow regime which is prone to complex viscous flow phenomena. They possess unique feather features and flexible wing structures which are postulated to help them fly nearly silently and stably at low speeds in a complex flow setting. Understanding the aerodynamics of owls could pave the way to enhance the future designs of small flying vehicles. Though it has been a focus of research over multiple decades, no conclusive agreement has been attained on the aerodynamic mechanisms associated with owl flight. Particularly, the aerodynamics of flapping owl flight is severely …
Investigating The Effect Of Dissolved Oxygen-Assisted Corneal Cross-Linking (Cxl) On Porcine Corneas, Julianni Dar
Investigating The Effect Of Dissolved Oxygen-Assisted Corneal Cross-Linking (Cxl) On Porcine Corneas, Julianni Dar
Electronic Theses and Dissertations
Corneal cross-linking is a clinical procedure that is known to stop the progression of keratoconus, an eye disease that affects the cornea’s structure, ultimately leading to vision loss in its advanced stages. The typical treatment plan includes riboflavin and UV-A exposure in the hope to increase the mechanical properties of the cornea. There are two types of CXL pathways, with Type-II CXL requiring oxygen. Naturally, the dissolved oxygen is limited in the cornea; therefore, limiting the effect of Type-II CXL. This study proposes to improve the Type-II CXL contribution by integrating dissolved oxygen during the standard CXL treatment used in …
Modeling Leaf Area Index And Canopy Height Using Growing Degree Days, Ellie J. Kuhn
Modeling Leaf Area Index And Canopy Height Using Growing Degree Days, Ellie J. Kuhn
Biological and Agricultural Engineering Undergraduate Honors Theses
As the global population increases and food security is recognized as a critical issue, crop growth prediction models help ensure the sustainability of reliable food sources. Using a prediction model based on temperature and simple, measurable field parameters, e.g., Leaf Area Index (LAI) or Canopy Height (Hcan), may allow farmers and others to intervene mid-season with fertilizer, irrigation, or other inputs to obtain a better harvest.
This study aims to create a general model that could predict LAI and Hcan values for numerous rice varieties using Growing Degree Days (GDD) as the time scale. The models use …
Development Of An Equation To Correlate Brite Tank Temperature And Pressure To Beverage Carbonation Concentration, Devyn Meyer
Development Of An Equation To Correlate Brite Tank Temperature And Pressure To Beverage Carbonation Concentration, Devyn Meyer
Biological and Agricultural Engineering Undergraduate Honors Theses
The Carbo Rock-It™ is an invention created to carbonate beverages at craft breweries. One of the Carbo Rock-It’s main features is that it does not add a significant volume of undissolved gas bubbles to the brite tank in which the beverage is carbonated. This may allow temperature and pressure readings from the brite tank to be used to determine the concentration of dissolved carbon dioxide in the beverage continuously in real time. This eliminates the need for manual samplings, which could save breweries time and allow for more controlled, accurate carbonation. The goal of this project was to develop an …
Atomic Force Microscopy Based Dna Sensing And Manipulation, Matthew Shubert
Atomic Force Microscopy Based Dna Sensing And Manipulation, Matthew Shubert
Mechanical Engineering Undergraduate Honors Theses
Sequencing DNA provides a positive impact for the biomedical community by understanding a wide variety of applications such as human genetics, disease, and pathogens. The reason the Arkansas Micro & Nano Systems lab is involved with research in DNA sequencing is due to the current, leading industry method. Nanopore sequencing was developed by Oxford Nanopore Technology in which its sequencing method separates double stranded DNA to electrically characterize individual nucleotides traveling through a charged nanopore. Unfortunately, nanopore sequencing uses biological materials that require a shelf life and drives high cost. Therefore, the Arkansas Micro & Nano Systems lab has developed …
Evaluation Of Triple Negative Breast Cancer Tumor Matrix, Alejandra Ham
Evaluation Of Triple Negative Breast Cancer Tumor Matrix, Alejandra Ham
Honors Theses
No abstract provided.
Exploring The Effects Of Varied Land Use On Elemental Concentrations Within Streams, Logan Jennings
Exploring The Effects Of Varied Land Use On Elemental Concentrations Within Streams, Logan Jennings
Biological and Agricultural Engineering Undergraduate Honors Theses
It is well documented that human activity influences the chemistry of surrounding waters. As such, it is possible that there is a link between land use within a watershed and the chemical composition of the stream. The objectives of this study are to determine if varied land use does affect the concentrations of macronutrients and trace elements present in the streams of Northwest Arkansas, and if so, to determine what extent urban and agricultural development are responsible for these changes. Water samples were collected across 19 streams in the Northwest Arkansas region between January and March of 2022. Water samples …
Nonlinear Optical Microscopy Assessment Of Tissue Structure And Chondrocyte Viability Of Articular Cartilage, Michael Le
Nonlinear Optical Microscopy Assessment Of Tissue Structure And Chondrocyte Viability Of Articular Cartilage, Michael Le
All Theses
Articular cartilage functions to protect the ends of bones by providing a surface that can withstand compressive forces and minimize friction during movement. Collagen fibers form the organizational backbone of the extracellular matrix (ECM) in cartilage. Proteoglycans within the ECM function to retain water and provide the tissue with the swelling pressure needed to withstand compressional forces. Chondrocytes, the only type of cell found in articular cartilage, produces these collagen fibers and proteoglycans to maintain the tissue structure and function. Significant injuries to articular cartilage can damage the chondrocytes and disrupt their ability to maintain homeostasis in the tissue. Therefore, …
Insect Antennae As Bioinspirational Superstrong Fiber-Based Microfluidics, Griffin J. Donley
Insect Antennae As Bioinspirational Superstrong Fiber-Based Microfluidics, Griffin J. Donley
All Theses
Nature is frequently turned to for inspiration for the creation of new materials. Insect antennae are hollow, blood-filled fibers with complex shape, and are cantilevered at the head. The antenna is muscle-free, but the insect can controllably flex, twist, and maneuver it laterally. To explain this behavior, a comparative study of structural and tensile properties of the antennae of Periplaneta americana (American cockroach), Manduca sexta (Carolina hawkmoth), and Vanessa cardui (painted lady butterfly) was performed. These antennae demonstrate a range of distinguishable tensile properties, responding either as brittle fibers (Manduca sexta) or strain-adaptive fibers that stiffen when stretched (Vanessa cardui …
Fabrication And Characterization Of Lactobacillus Crispatus Containing Bioprints For Bacterial Vaginosis Application., Anthony J. Kyser
Fabrication And Characterization Of Lactobacillus Crispatus Containing Bioprints For Bacterial Vaginosis Application., Anthony J. Kyser
Electronic Theses and Dissertations
Bacterial vaginosis (BV) is a condition in which healthy lactobacilli are replaced by an overabundance of pathogenic bacteria in the female reproductive tract. Current antibiotic treatments often fail to “cure” infection, resulting in recurrence in more than 50% of women, 6 months post-treatment. Recently, probiotics have demonstrated promise to restore vaginal health; however, as with other active agents, delivery requires once-to-twice daily administration. Recently, three-dimensional (3D)-bioprinting has enabled the fabrication of well-defined cell-laden architectures with tunable agent release, thereby presenting a novel approach with which to deliver probiotics. One promising bioink, gelatin alginate, was selected for study, due to its …
Toward Sustainable Urban Food Production: Integrating Hydroponic Cultivation With Wastewater Reuse, Amanda Tan
Toward Sustainable Urban Food Production: Integrating Hydroponic Cultivation With Wastewater Reuse, Amanda Tan
All Theses
The growing crisis of freshwater scarcity together with the increasing global food demand directs attention to the need for alternative water sources for agriculture. The research presented here engages this need by carrying out indoor cultivation of food crops in Nutrient Film Technique (NFT) hydroponic systems using residential wastewater. Randomized single factor experimental designs were used to investigate the physiochemical and microbiological makeup of the hydroponic nutrient solution throughout the cultivation cycle and their relationship with different plant development parameters.
For agricultural wastewater reuse to be of low risk to human health there must be some advancement in pathogen monitoring …
Cyanobacterial And Microcystin Response To Nutrient Additions At Lake Fayetteville Throughout The 2021 Growing Season, Lillie Haddock
Cyanobacterial And Microcystin Response To Nutrient Additions At Lake Fayetteville Throughout The 2021 Growing Season, Lillie Haddock
Graduate Theses and Dissertations
Harmful Algal Blooms (HABs) are becoming a global concern due to their increasing distribution, frequency, intensity, and the occurrence of toxins. While it is known that eutrophication influences algal blooms, there is less known about what triggers these HABs to produce toxins, especially microcystin. In this study, we conducted 21 community bioassays at Lake Fayetteville, a hypereutrophic reservoir in Fayetteville, Arkansas, from April-November 2021 to examine how the addition of phosphorous and nitrogen influence cyanobacteria concentrations, microcystin concentrations, and microcystin toxin production. These experiments included a control, nitrogen (1.0 mg/L as KNO3), low phosphorus (0.025 mg/L as K2HPO4), high phosphorus …
An Engineering And Economic Analysis Of The Implementation Of The Carbo Rock-It, Lydia Huck
An Engineering And Economic Analysis Of The Implementation Of The Carbo Rock-It, Lydia Huck
Graduate Theses and Dissertations
The patented Carbo Rock-It system was developed to carbonate beverages for the craft brewing industry with the goal of reducing time, cost, and environmental impacts of the carbonation process. This research field tests, models, analyzes and optimizes the engineering and economic performance of a commercial-scale Carbo Rock-It placed in production at an operating brewery (Core Brewing and Distilling Co., Springdale, AR). Field tests indicated that the Carbo Rock-It was able to successfully carbonate twelve 120 BBL batches of Scarlet Letter Seltzer to 3.0 vol/vol while meeting all beverage quality and safety requirements. The Carbo Rock-It was able to reduce carbonation …
Nanoparticle Quantification And Distribution On Fluorescently Coated Angioplasty Balloons, Allison Dobson Zieschang
Nanoparticle Quantification And Distribution On Fluorescently Coated Angioplasty Balloons, Allison Dobson Zieschang
Honors Theses
No abstract provided.
The Tin Man Needs A Heart: A Proposed Framework For The Regulation Of Bioprinted Organs, Linda Foit
The Tin Man Needs A Heart: A Proposed Framework For The Regulation Of Bioprinted Organs, Linda Foit
Fordham Law Review
Each day, seventeen people die in the United States while waiting for an organ transplant. At least part of this need could be met by bioprinting, a technology that allows the on-demand production of custom-sized organs from a patient’s own cells. The field of bioprinting is progressing rapidly: the first bioprinted organs have already entered the clinic. Yet, developers of bioprinted organs face significant uncertainty as to how their potentially lifesaving products will be regulated—and by which government agency. Such regulatory uncertainty has the potential to decrease investment and stifle innovation in this promising technological field. This Note examines how …