Arts and Humanities Commons^™

Structured Light For Three-Dimensional Microscopy, Leo G. Krzewina

USF Tampa Graduate Theses and Dissertations

The conventional light microscope is an indispensable tool for many physical and life science applications, but is of limited usefulness for three-dimensional imaging due to its increasingly narrow depth of field at high magnifications. Focused regions may be obscured by defocused neighbors or noise from extraneous light sources and subsurface scattering. By rejecting light originating from outside the depth of focus it is possible to minimize these problems. When a contiguous series of such focused slices, or optical sections, are obtained along an axis of an extended object they may be combined to form a complete, focused three-dimensional surface image. …

Occupancy And Function Of The Hepatic Hmg-Coa Reductase Promoter, William Raymond Lagor

USF Tampa Graduate Theses and Dissertations

HMG-CoA reductase (HMGR) catalyzes the rate controlling step in cholesterol production. This enzyme is highly expressed in the liver where it is subject to extensive hormonal and dietary regulation. This study was undertaken to examine the occupancy and function of the hepatic HMGR promoter in regards to insulin and sterol regulation. HMGR protein and mRNA are substantially decreased in diabetic animals and rapidly restored by administration of insulin. Nuclear run-on assays revealed that HMGR transcription was substantially reduced in the diabetic rats, and fully restored within two hours after insulin treatment. In vivo footprinting revealed several areas of protein binding …

Variation In Pediatric Gastroenteritis Admissions Among Florida Counties, 1995-2002, Jean Lee

USF Tampa Graduate Theses and Dissertations

Background: Hospitalizations for pediatric gastroenteritis are considered potentially avoidable and are used to monitor access and quality of primary care for children. Previous reports have found pediatric gastroenteritis admissions higher in Florida compared to the South and the nation.Purpose: The purpose of this project was to explore variation in county admission rates for pediatric gastroenteritis related to non-clinical factors in Florida during 1995-2002. Specific aims included identifying the unique contributions of county socioeconomic characteristics and availability of primary care resources to annual county pediatric gastroenteritis hospital admission rates. Method: The study was retrospective and longitudinal assessing variation in annual county …

The Case For High-Order, Pleistocene Sea-Level Fluctuations In Southwest Florida, Paul Octavius Knorr

USF Tampa Graduate Theses and Dissertations

Florida's Plio-Pleistocene strata record episodes of sea-level highstands. The age of the strata is often ambiguous as there are no consistently reliable dating techniques that can be unequivocally applied to many of the units. The lack of preservation of continuous Plio-Pleistocene sedimentary sequences is a consequence of Pleistocene mean sea-levels not flooding peninsular Florida, the low volume of sedimentary supply, and the lack of new accommodation space. This study investigates a 6 m cyclothem-type set of six shallow-marine shell beds separated by five subaerially exposed packstone beds. These strata are part of the biostratigraphically-defined early Pleistocene (1.1 --

1.6 Ma) …

Three Science Teacher Educators With Elementary Teaching Experience: What Do We Bring To Science Teacher Education?, Cyndy S. Leard

USF Tampa Graduate Theses and Dissertations

This research explored the common experiences, values, and beliefs of three science teacher educators with elementary teaching experience and how these commonalities influenced their thinking about teaching preservice elementary teachers. This qualitative study used a life story approach and included an autobiographical component. Data were collected through interviews, observations, and archival records. The findings were shared in eight chronologically ordered vignettes that portrayed an individual representing a composite of the participants. The participants shared numerous life experiences including: growing up in small towns, having older parents, being part of a two sibling family, having strong female role models, learning science …

Measuring Social Stressors In Organizations: The Development Of The Interpersonal Conflict In Organizations Scale (Icos), Valentina Bruk Lee

USF Tampa Graduate Theses and Dissertations

Interpersonal conflict in organizations has been recognized as a leading social stressor across occupations with detrimental effects on employee well-being and organizational outcomes. However, reliable and valid measures of conflict are scarce and even the most widely used scales are limited by weaknesses in construct definition. In order to address the need for an improved measurement tool, the 63-item Interpersonal Conflict in Organizations Scale (ICOS) was developed. The ICOS was based on a comprehensive conceptualization of conflict that defines the construct on the basis of three definitional components: disagreement, interference, and negative emotion (Barki & Hartwick, 2004). In addition, the …

N-Thiolated Β-Lactam Antibiotics: Synthesis And Structure-Activity Studies Of C3- Oxygenated Derivatives And Attachment To New, Functionalized Caprolactone Monomers And Polymers, J Michelle Leslie

USF Tampa Graduate Theses and Dissertations

N-Thiolated beta-lactams are a new class of anti-MRSA and anti-Bacillus agents that have recently been reported by our laboratories. From previous studies performed in our laboratories, it is believed that the N-thiolated beta-lactams exert their antimicrobial activity through a unique mode of action that is completely unlike that of classical beta-lactam antibiotics. In the first chapter of this dissertation, a review of previously prepared N-thiolated beta-lactam analogues and their mode of action is presented. In the second chapter, the synthesis of seven different C3-oxygenated derivatives is described. These analogues were tested for antibacterial activity against Staphylococcus aureus, nine different strains …

Electrically Charged Sol-Gel Coatings For On-Line Preconcentration And Analysis Of Zwitterionic Biomolecules By Capillary Electrophoresis, Wen Li

USF Tampa Graduate Theses and Dissertations

Novel on-line methods are presented for the extraction, preconcentration and analysis of zwitterionic biomolecules using sol-gel-coated columns coupled to a conventional UV/visible detector. The presented approaches do not require any additional modification of the commercially available standard CE instrument. Extraction, stacking, and focusing techniques were used in the preconcentration procedures. The positively charged sol-gel coatings were created using N-octadecyldimethyl[3-(trimethoxysilyl) proply]ammonium chloride (C18-TMS) in the coating sol solutions. Due to the presence of a positively charged quaternary ammonium moiety in C18-TMS, the resulting sol-gel coating carried a positive charge. The negatively charged sol-gel coatings were due to the presence of sulfonate …

Control Of Lysogeny In Marine Bacteria: Studies With Φhsic And Natural Populations, Amy K. Long

USF Tampa Graduate Theses and Dissertations

Viruses have an estimated global population size of 10 to the 31st, with a significant proportion found in the marine environment. Viral lysis of bacteria affects the flow of carbon in the marine microbial food web, but the effects of lysogeny on marine microbial ecology are largely unknown. In this thesis, factors that influence the control of lysogeny were studied in both the phiHSIC/Listonella pelagia phage-host system and in bacterioplankton populations in the Gulf of Mexico. Using macroarrays dotted with phiHSIC amplicons, viral gene expression over the course of a synchronous infection experiment was measured. Early, middle, late, and continually …

Breathing Easier: Ethnographic Study Of Acute Respiratory Infection In Children In Rural Ecuador, John S. Luque

USF Tampa Graduate Theses and Dissertations

This dissertation research utilizes anthropological methods to determine the degree to which the signs and symptoms female caregivers identify as causes of acute respiratory infections (ARIs) in under-fives in rural Ecuador correspond with Western biomedical categories. By employing both a semi-structured medical history questionnaire and more open-ended ethnographic methods such as in-depth informant interviews and focus group research, the researcher identifies the factors which determine timely health care-seeking behaviors of female caregivers in this case study. Economic factors such as the cost of medications and lost work hours were determined to be the primary financial obstacles for timely health care-seeking. …

Gender Differences During Heat Strain At Critical Wbgt, Christina L. Luecke

USF Tampa Graduate Theses and Dissertations

Heat stress is influenced by environmental conditions, workload and clothing. A critical environment is the upper limit of compensable heat stress for a given metabolic rate and clothing ensemble. The physiological strains associated with heat stress are core and skin temperatures, heart rate and physiological strain index (PSI). Because heat dissipation mechanisms may differ between men and women, there may be gender differences in the critical environment and the associated physiological variables. Gender differences were explored between acclimated men (n = 20) and women (n = 9) at the upper limit of compensable heat stress. Participants walked on a motorized …

Virginia Woolf And The Persistent Question Of Class: The Protean Nature Of Class And Self, Mary C. Madden

USF Tampa Graduate Theses and Dissertations

From the beginning of her career, Virginia Woolf moves beyond the perspective of her inherited class position to challenge a damaging class system. She increasingly recognizes the extent of her own complicity in the creation and maintenance of class structures supporting patriarchy, war, and British imperialism. Highlighting ambiguities inherent in the very category of class, she acknowledges the limiting "boxes" of language itself in attempts to rethink class. For Woolf, class is not monolithic but internally differentiated by gender and race. Examining Woolf's early work in relation to class theory shows that throughout her career Woolf interrogates the imbrication of …

Explaining Gender Differences In Salary Negotiations, Meisha -Ann Martin

USF Tampa Graduate Theses and Dissertations

The current study explores the effects of gender on salary negotiation behaviors and expectancies and the relationship between these variables and starting salary outcomes. College students from a variety of different majors were surveyed prior to and then approximately two to four months after graduation. Though there was no gender difference in final salary or difference between initial and final salary offer, men reported using more aggressive and active salary negotiation behaviors. The results also suggest that men may have felt more empowered in the salary negotiation context. They expected higher salaries than women did, anticipated less discomfort and believed …

Investigation Of The Effects Of Increased Levels Of O-Glcnac Protein Modification On Protein Kinase C And Akt, Jason Aaron Matthews

USF Tampa Graduate Theses and Dissertations

O-linked N-acetylglucosamine (O-GlcNAc) is an abundant and ubiquitous post-translational modification that has been shown to play a role in regulating a variety of intracellular processes. The pathway responsible for generating the O-GlcNAc modification, the hexosamine biosynthetic pathway (HBP), has also been shown to affect the activity and translocation of certain protein kinase C (PKC) isoforms. To investigate if the effects of HBP flux on PKC translocation observed by others is related to the O-GlcNAc modification, O-GlcNAc levels in human astroglial cells were elevated using four separate O-GlcNAc modulating agents followed by analysis of cytosol and membrane concentrations of PKC-epsilon, -alpha, …

Moderating Effect Of Negative Affectivity On The Job Satisfaction-Turnover Intentions And Justice-Turnover Intentions Relationships, Joseph J. Mazzola

USF Tampa Graduate Theses and Dissertations

Although many people experiences dissatisfaction with their jobs at some time or another, not all of them quit their jobs because of it. Why do some employees simply continue to work in an unsatisfying environment? In this study, it is hypothesized that part of the reason is based on the individual's personality, specifically their scores on positive and negative affectivity. Results showed that neither NA nor PA moderated the relationship between job satisfaction and turnover intentions/job search, contrary to previous literature. This study suggests that the moderation effect of personality on this relationship, either does not exist or is more …

Quantitative Biological Microscopy By Digital Holography, Christopher J. Mann

USF Tampa Graduate Theses and Dissertations

In this dissertation, improved techniques in digital holography, that have produced high-resolution, high-fidelity images, are discussed. In particular, the angular spectrum method of calculating holographic optical field is noted to have several advantages over the more commonly used Fresnel transformation or Huygens convolution method. It is observed that spurious noise and interference components can be tightly controlled through the analysis and filtering of the angular spectrum. In the angular spectrum method, the reconstruction distance does not have a lower limit, and the off-axis angle between the object and reference waves can be lower than that of the Fresnel requirement, while …

Electrogenetherapy Of Established B16 Murine Melanoma By Using An Expression Plasmid For Hiv-1 Viral Protein R, Andrea Nicole Mccray

USF Tampa Graduate Theses and Dissertations

Novel therapies and delivery methods directed against malignancies such as melanoma, and particularly metastatic melanoma, are needed. The HIV-1 accessory protein Vpr (viral protein R) has previously been demonstrated to induce G2 cell cycle arrest as well as in vitro growth inhibition/killing of numerous tumor cell lines. In vivo electroporation has been utilized as an effective delivery method for pharmacologic agents as well as DNA plasmids that express "therapeutic" proteins and has been targeted to various tissues including malignant tumors. In this study, we assessed the ability of electroporation-mediated delivery of Vpr plasmid (pVpr) to induce growth attenuation or complete …

Analysis Of Acoustic Emission In Cohesionless Soil, Jeyisanker Mathiyaparanam

USF Tampa Graduate Theses and Dissertations

Acoustic emission is a widely used nondestructive technique for identification of structural damage. The AE technique relies on transient energy waves generated by the materials during their failure. As for soils, the basic causes of acoustic emission are the mechanisms which are responsible for shearing of soils. Mobilization of shear strength within a soil itself and the interaction of the soil with the adjacent natural or construction materials are directly related to the level of acoustic emission in soils. It is envisioned that acoustic emission signals in deforming soils can be used as an early warning sign in real time …

The Role Of Social Structural And Social Contextual Factors In Shaping Chronic Disease And Chronic Disease Risk Behavior: A Multilevel Study Of Hypertension, General Health Status, And Mental Distress, Caroline Mae Mckay

USF Tampa Graduate Theses and Dissertations

At present there is a reliance on behavioral interventions that have been limited in their effectiveness to reduce the public health burden of chronic disease, partly because the effects of social context on the initiation and maintenance of health behaviors is not incorporated into public health policy and practice. Yet current research indicates that there are macro-level structural and contextual influences on population health that cannot be reduced to individual or compositional effects. This study investigated the associations between social structural factors, community social context, individual characteristics, and self-reported correlates of disease. Distal influences included social structural inequalities such as …

Crystal Engineering Of Novel Pharmaceutical Forms, Jennifer Anne Mcmahon

USF Tampa Graduate Theses and Dissertations

In the context of pharmaceutical development, it is abundantly clear that there is a need for greater understanding and control of crystalline phases. The field of crystal engineering is poised to address such issues and has matured into a paradigm for the supramolecular synthesis of new compounds with desired properties. Crystal structures are unpredictable by nature, however, the interactions that lead to crystal formation are becoming much more predictable. By means of model compound studies, the delineation of the hierarchy of hydrogen bonding between complementary functional groups or supramolecular heterosynthons can be accomplished. Competitive co-crystallization studies along with data extracted …

The Ethics Of The Spirit In Galatians: Considering Paul’S Paranesis In The Interpretation Of His Theology, Steven Douglas Meigs

USF Tampa Graduate Theses and Dissertations

The faith versus works dichotomy that has been a foundation of Protestant Christianity for centuries is derived in large part from a flawed understanding of Paul's theology in the epistles to the Galatians and Romans. In the wake of WWII, scholars began reexamining Pauline theological constructs and proposing new ways of understanding Paul's arguments regarding faith and works. James D. G. Dunn dubbed this dialogue the "new perspective." This paper will contribute to one particular aspect of new perspective dialogue: understanding the relation of the paranetic material in the final two chapters of Galatians to Paul's theological arguments in the …

Pediatric Healthcare Providers’ Screening Practices: Impact Of Training On Early Identification Of Autism, Aja M. Meyer

USF Tampa Graduate Theses and Dissertations

This study explored the effectiveness of the Autism System of Care (ASC) trainings by measuring change in pediatric healthcare providers' method of identifying young children at-risk for autism spectrum disorders. The majority of participants were pediatricians working in either hospitals or clinics who voluntarily participated in the training. A pretest-posttest nonequivalent-groups design was used in this study. Pre- and post-test questionnaires were used to measure change in participants' screening practices. Due to a small number of participants, most findings from the study were not statistically significant. The small number of healthcare providers who participated in the ASC training was a …

Evaluation Of Aashto Design Specifications For Cast-In-Place Continuous Bridge Deck Using Remote Sensing Technique, Ebrahim Mehranipornejad

USF Tampa Graduate Theses and Dissertations

This research project concerns the construction, testing, and remote health monitoring of the first smart bridge structure in Florida, the East Bay bridge in Gibsonton, Hillsborough County. The East Bay Bridge is a four span, continuous, deck-type structure with a total length of 120' and width of 55'. The superstructure consists of an 18'' cast-in-place reinforced concrete slab, and is supported on pre-stressed pile bents, each consisting of 5 piles. The smart sensors used for remote health monitoring are the newly emerged Fabry --Perot (FP) Fiber Optic Sensors, and are both surface-mounted and embedded in the concrete deck.Static and Dynamic …

Regression Approach To Software Reliability Models, Abdelelah M. Mostafa

USF Tampa Graduate Theses and Dissertations

Many software reliability growth models have beenanalyzed for measuring the growth of software reliability. In this dissertation, regression methods are explored to study software reliability models. First, two parametric linear models are proposed and analyzed, the simple linear regression and transformed linearregression corresponding to a power law process. Some software failure data sets do not follow the linear pattern. Analysis of popular real life data showed that these contain outliers andleverage values. Linear regression methods based on least squares are sensitive to outliers and leverage values. Even though the parametric regression methods give good results in terms of error measurement …

Developing Culture Conditions To Study Keratocyte Phenotypes In Vitro, Kurt Musselmann

USF Tampa Graduate Theses and Dissertations

The corneal wound healing response involves the activation of keratocytes to proliferate from a quiescent phenotype. The mitogens that cause the initial transformation of the quiescent keratocytes to the active phenotype have not been identified. Even though serum is commonly used to replicate this in vitro, the cornea is avascular and therefore likely not exposed to serum. In the first part of this dissertation, a DMEM/F12 extract of corneal stromas was made and shown to stimulate keratocyte proliferation in both a dose-dependent and cell-density dependent manner. This extract contains mitogens that differ from the mitogens present in serum based on …

Thermal Analyses Of Hydrophilic Polymers Used In Nanocomposites And Biocompatible Coatings, Kadine Mohomed

USF Tampa Graduate Theses and Dissertations

ABSRACT: This research focuses on two hydrophilic polymers that form hydrogels when they sorb water: Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly(2,3-dihydroxypropyl methacrylate) (PDHPMA). Present work in the field obviated the need to properly characterize the thermal and dielectric properties of these materials.The dielectric permittivity, e', and the loss factor, e", of dry poly(2-hydroxyethyl methacrylate) and poly(2,3-dihydroxypropyl methacrylate) were measured using a dielectric analyzer in the frequency range of 0.1Hz to 100 kHz and between the temperature range of -150 °C to 275°C. The dielectric response of the sub-Tg gamma transition of PHEMA has been widely studied before but little to no …

Distribution And Habitat Characterization Of The Florida Burrowing Owl In Non-Urban Areas, Mark S. Mueller

USF Tampa Graduate Theses and Dissertations

The full geographic distribution and habitat use of the Florida Burrowing Owl, a state "Species of Special Concern," is not well-understood, particularly in remote, non-urban areas. This thesis aimed to expand and improve knowledge about non-urban burrowing owls. We first compiled databases of historic sighting observations. Fieldwork verified and updated existing breeding observation point records and also yielded new breeding locations. Using a GIS, we characterized observed land use, landcover, relevant soil attributes, projected future land use and managed area status for selected points. We quantified landcover within biologically-determined buffer distances around burrows from our own field-verified records. Using standard …

A Design Of Experiments Study Of Procedure For Assembling Bascule Bridge Fulcrum, Cuong Q. Nguyen

USF Tampa Graduate Theses and Dissertations

A bascule bridge is a type of movable bridge that can be opened or closed to facilitate the movement of water-borne traffic such as ships and yachts. Trunnion-Hub-Girder (THG) assembly plays a role as a fulcrum in the bascule bridge. To make the fulcrum, the trunnion is shrink-fitted into the hub, and then the trunnion-hub assembly is shrink-fitted into the girder. Hundreds of thousands of dollars could be lost due to failures during this step. Crack formations in the hubs of various Florida bascule bridges during assembly led the Florida Department of Transportation to commission a project with USF professors …

The Contribution Of The Neighborhood Context To Social Disparities In Access To Health Care Among Sexually Experienced Adolescent Females, Jodi Nearns

USF Tampa Graduate Theses and Dissertations

Access to health care is an important resource for sexually experienced adolescent females in the prevention of unintended pregnancy and sexually transmitted infections, including HIV. However, a paucity of research exists regarding the extent to which social disparities in access to health care exist among this vulnerable population of adolescents, including the potential contribution of the neighborhood context. Therefore, the primary aims of this dissertation were to examine (1) the extent to which racial and socioeconomic disparities in access to health care exist among sexually experienced adolescent females, (2) the extent to which access to health care among sexually experienced …

Effects Of Translocation On The Florida Burrowing Owl, Athene Cunicularia Floridana, Per Anders Nixon

USF Tampa Graduate Theses and Dissertations

At present, the Florida Burrowing Owl is being threatened by extensive habitat development throughout their small range in the state. Unfortunately, developers are able to collapse burrowing owl burrows during the non-breeding season and flush the owls from an area. In other areas such as Arizona and British Columbia translocation is being utilized to mitigate the effects of development on burrowing owls. In March 2006, the only translocation of burrowing owls in Florida was conducted by Mosaic Phosphate Company. The purpose of this thesis was to elucidate the effects of translocation on Florida burrowing owls. Topics of research include activity …