Arts and Humanities Commons^™

Non-Invasive Manipulation Of Membrane Potential In Intact Living Cells, Robin Dando

USF Tampa Graduate Theses and Dissertations

All living cells contain the electrogenic enzyme Na/K ATPase, whose function is to pump ions against the electrochemical gradient, in order to provide potential energy which is later used for cellular processes such as action potentials, muscle contraction and facilitated transport. Using a technique developed in our lab, exploiting the molecule's voltage dependence, it is possible to increase this pump function by many folds. Optical measurement of the membrane potential of living cells was made using a potentiommetric dye, with successful manipulation of the ionic concentration and membrane potential reported. Additional supporting results are presented, along with extension of this …

Interagency Collaboration And Communication: Funders And Service Providers Working Together To Create A Management Service Organization, Beth E. Eschenfelder

USF Tampa Graduate Theses and Dissertations

Charitable nonprofit organizations are growing in number and scope, but the body of literature and knowledge about communication within these organizations is limited. Today, charitable organizations face a flurry of economic and environmental factors that challenge their continued survival: growing community needs, mounting funding cuts from all segments of government, increased competition for funding, a limited pool of dedicated community leaders to serve on boards, decreased charitable giving for some causes, and persistent calls for greater accountability. As nonprofits explore new ways of coping with these socioeconomic pressures---such as reorganization, consolidation, mergers, and management service organizations---there is a clear need …

The Model-Based Systematic Development Of Logis Online Graphing Instructional Simulator, Darrel R. Davis

USF Tampa Graduate Theses and Dissertations

This Developmental Research study described the development of an interactive online graphing instructional application and the impact of the Analysis Design Development Implementation Evaluation (ADDIE) model on the development process. An optimal learning environment was produced by combining Programmed Instruction and Adaptive Instruction principles with a graphing simulator that implemented guided contingent practice. The development process entailed the creation and validation of three instruments measuring knowledge, skills, and attitudes, which were components of the instruction.

The research questions were focused on the influence of the ADDIE model on the development process and the value of the LOGIS instructional application. The …

Examining The Distinction And Concordance Between Implicit Measures Of Alcohol Expectancies: Toward Agreement On Their Meaning And Use, Maureen C. Below

USF Tampa Graduate Theses and Dissertations

Alcohol expectancies have traditionally been measured with explicit self-report questionnaires, but in recent years implicit measures have also been used to explore the tenets of expectancy theory. The basic psychometric properties of reliability and validity have not been established for most implicit tasks, and the convergent validity of different implicit measures has not been explored. Despite these shortcomings, many researchers continue to treat implicit tasks as reliable and valid assessment tools. To address reliability and validity of implicit measures, 218 undergraduate women and men were recruited from the University of South Florida to examine the psychometric properties of and concordance …

Political Pedagogy And Art Education With Youth In A Street Situation In Salvador, Brazil: An Ethnographic Evaluation Of The Street Education Program Of Projeto Axé, Lance A. Arney

USF Tampa Graduate Theses and Dissertations

Projeto Axé is a non-governmental organization that carries out political-pedagogical work and art education for children and adolescents living in a street situation in Salvador, Bahia, Brazil. I conducted an exploratory ethnographic study with Projeto Axé's Street Education program in order to observe its day-to-day activities and to conduct a utilization-focused program evaluation (Patton 1997) of its pedagogical praxis. This thesis will describe how ethnographic fieldwork methods are interwoven with the political-pedagogical approaches of Projeto Axé's Street Education program and explain how street educators attempt to construct citizenship with participants in the Street Education program. Findings are based on an …

Cost/Effectiveness Analysis Of Obtaining Operational Estimates Of Reference Evapotranspiration, Peninsular Florida, Usa, Michael G. Kittridge

USF Tampa Graduate Theses and Dissertations

The objective of this study is to conduct a cost/effectiveness analysis of the computation of reference evapotranspiration (ETo) in the peninsular of Florida. A meteorological station on the Fort Meade Mine in Polk County, Florida was used to provide data for the calculation of ETo. Five ETo equations were tested to determine the accuracy and cost/effectiveness to the fully measured ASCE Penman-Monteith (Full ASCE-PM) equation on daily, monthly, and annually time steps. The ETo equations ranged in amounts of parameters from the Full ASCE-PM to the Hargreaves. The energy terms accounted for approximately 90% of the total ETo flux. Solar …

Clear Speech Effects For Vowels Produced By Monolingual And Bilingual Talkers, Teresa Demasi

USF Tampa Graduate Theses and Dissertations

'Clear speech' is a speaking style that talkers often employ when they know they may have trouble being understood, as when speaking in noise or to a person with a hearing loss. When 'clear speech' produced by native talkers is presented in noise to native listeners, it has been shown to be about 10-15 percentage points more intelligible, on average, than normally produced speech. Recent research has shown that bilingual listeners may experience a smaller intelligibility benefit than monolingual listeners from 'clear speech' produced by monolingual talkers. The present study compares the ability of monolingual and bilingual talkers to produce …

The Effects Of Decision-Making And Leadership Styles On Relationships And Perceived Effectiveness In The University Development Context, Rachael K. Van Loveren

USF Tampa Graduate Theses and Dissertations

This study examined how employees' perceptions of leadership, decision-making, and relationships are associated with their perception of a development operations' effectiveness. Deans, development officers, central development staff, and unit development staff at the University of South Florida were surveyed via email. The results indicated that employees' perceptions of leadership, decision-making, and relationships are strongly related to their perceived job satisfaction, trust, commitment, and control mutuality and consequently their perception of the development operation's effectiveness.

An Evaluation Of Movement Patterns And Effects Of Habitat Patch Size On The Demography Of The Florida Mouse (Podomys Floridanus), Irmgard Lukanik

USF Tampa Graduate Theses and Dissertations

Habitat degradation by humans has been the main reason for the decline in numbers of P. floridanus, the only mammal indigenous to the state of Florida, in the past century. The mouse inhabits what remains of scrub and sandhill associations, which are characterized by patches of sandy soils within a more mesic landscape. It has long been accepted that small populations are more prone to decline and extinction than are larger ones as a result of environmental fluctuations. I hypothesized that the demography of a population of P. floridanus would be affected by a restriction in numbers through habitat …

Effects Of Clear Speech And Linguistic Experience On Acoustic Characteristics Of Vowel Production, Michelle Bianchi

USF Tampa Graduate Theses and Dissertations

The present study investigated the hypothesis that later and/or early learners of English as a second language may exhibit an exaggerated or restricted degree of change in their production performance between clear and conversational speech styles for certain acoustic cues. Monolingual English talkers (MO), early Spanish-English bilinguals (EB) and late Spanish-English bilinguals (LB) were recorded using both clear and conversational speaking styles. The stimuli consisted of six target vowels /i, I, e, E, ae/ and /a/, embedded in /bVd/ context. All recorded target-word stimuli were isolated into words. Vowel duration was computed, and fundamental frequency (F0), and formant frequency values …

Geomorphology Of Submarine Spring West Of Fort Myers, Florida, Shihadah M. Saleem

USF Tampa Graduate Theses and Dissertations

In March of 2000, March of 2001, and April of 2002, multibeam bathymetry and backscatter data were collected, which revealed several low-temperature hydrothermal submarine springs in the Mudhole Submarine Springs (MHSS) area that were investigated by SCUBA divers. High-resolution multibeam sonar provides a precise way of defining the geomorphology of the seafloor. The bathymetry data were used to understand (1) vent geomorphology and how it varied from vent to vent; (2) spatial patterns of active vents compared to extinct vents and known land springs identified by Kohout (1977) and Breland (1980); and (3) potential correlations between geochemical and geomorphological characteristics …

Explaining Drinking Patterns And Heavy Drinking Among Racial And Ethnic Subgroups In The United States, Michael S. Caudy

USF Tampa Graduate Theses and Dissertations

The study of racial differences in the consumption of alcohol and the prevalence of alcohol-related problems has clearly matured in recent years. Researchers have moved away from single-factor explanations and are beginning to develop and test theories focusing on the complex interplay of psychological, historical, cultural, and social factors that describe and explain alcohol use among racial and ethnic subgroups in the United States. The current study continues this maturation process by further examining the complex interaction effects of predictor variables that have established their utility in explaining racial/ethnic subgroup differences in alcohol consumption and alcohol-related problems. This study analyzes …

Improving Pediatric Cardiology Consultation Methods By Introducing Digital Interactive 3-D Heart Models: A Proof Of Concept Study, Adam Verigan

USF Tampa Graduate Theses and Dissertations

The purpose of a pediatric cardiology consultation is to inform, or educate, the patient and family of all aspects surrounding a certain congenital heart defect. Consultation education methods and materials may include verbal descriptions, two-dimensional (2-D) heart diagrams, and take-home pamphlets. Because the human heart is a complex three-dimensional (3-D) object, the problem lies within the clarity to which these methods are performed by the doctors and understood by the patients and families. Therefore, during a consultation the cardiologist must a) possess the ability to describe a defect visually as well as verbally and b) ensure that the patient and …

Understanding And Closing The Gaps: A Gap Audit Approach Linking Archaeology And Land Acquisition Strategies In Florida, Lori D. Collins

USF Tampa Graduate Theses and Dissertations

The population in Florida is projected to double over the next 50 years. Large land areas now in rural settings will become residential and urban areas. More than seven million acres of agriculture and open space will convert to housing developments, shopping malls, and business space. At stake are natural and cultural resources, which are lost or fragmented in this growth process. New planning measures are called for in order to grow in ways that minimize and least impact resources.

Archaeological value in preservation projects is often examined after priorities for natural resources have been set, relegating archaeology to a …

The Use Of Microarrays In The Detection Of The Gene Expression Of Ribulose- 1,5- Bisphosphate Carboxylase/Oxygenase (Rubisco) In The Marine Environment, Kathryn Lafaye Bailey

USF Tampa Graduate Theses and Dissertations

The Calvin-Benson-Bassham (CBB) pathway is the primary pathway for the entry of inorganic carbon in the biosphere. Autotrophic organisms use this cycle to ultimately convert CO₂ into carbohydrates using a key enzyme known as ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene that encodes for the large subunit of RubisCO is rbcL and detection of its expression can be used to determine the autotrophic organisms present in the environment. Recently, microarrays have been used to study functional gene expression from environmental samples such as those obtained from sediments and soil. The purpose of this thesis is to combine microarray technology and rbcL …

Expanded Tonality: The Treatment Of Upper And Lower Leading Tones As Evidenced In Sonata "Undine,” Iv By Carl Reinecke, Joshua Blizzard

USF Tampa Graduate Theses and Dissertations

In the Romantic period, expanded tonality offers a creative challenge to composers as they explore new ways of establishing the hierarchy of pitches and utilizing the chromatic and diatonic resources. Prominent compositional techniques of this period include the use of linear harmony with less clearly defined root movements, the structural placement of dominant function, new approaches that redefine tonal stability, motivic treatment that generates harmony and form, flexible treatment of rhythm and meter, and functional treatment of chromatic pitches. This study explores the ways in which characteristics of the Romantic period are influenced by the upper/lower leading tone and the …

Parenting Style, Home-Based Involvement, And Educational Expectations Of Black Parents: Their Roles In The Development Of Pre-Literacy Readiness Of Black Children, Iravonia Rawls

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate the relationship of parenting style, home-based involvement, parents’ educational expectations and pre-literacy readiness. Sixty-two preschool children and his or her parent or guardian participated in this study of: 1) The relationship between parenting style and pre-literacy readiness of Black children enrolled in Head Start programs; 2) The relationship between parents’ educational expectations of Black children enrolled in Head Start programs and preliteracy readiness; 3) The relationship between home-based involvement of Black parents and levels of pre-literacy readiness of their children enrolled in Head Start programs; and 4) The relationship between the predictor …

Identity Politics: Postcolonial Theory And Writing Instruction, Toni P. Francis

USF Tampa Graduate Theses and Dissertations

In this dissertation I intend to apply postcolonial theory to primary pedagogical and administrative concerns of the writing program administrator. Writing Program Administrators, or WPAs, take their responsibilities seriously, remaining cognizant of both the negative and positive repercussions of the pedagogical decisions that take shape in the scores of composition classrooms they administer. This dissertation intends to infuse the WPA position with the ethos of scholarly praxis by historicizing and contextualizing the field of composition, and by placing the teaching of writing within the historical memory of slavery and colonialism. Sound WPA research is theoretically informed, systematic, principled inquiry that …

Self-Potential Anomalies And Co2 Flux On Active Volcanoes: Insights From Time And Spatial Series At Masaya, Telica, And Cerro Negro, Nicaragua, Heather L. Lehto

USF Tampa Graduate Theses and Dissertations

Considerable effort worldwide has gone into monitoring heat and mass transfer at active volcanoes, as this information may provide clues about changes in volcanic activity and impending eruptions. One method used is the self-potential (SP) method, which has been employed on volcanoes to map hydrothermal systems and structural features and to monitor changes in the hydrothermal system due to volcanic activity. Continuous monitoring of SP has been employed on a few volcanoes and has produced encouraging results. This study presents new time series data collected from continuous monitoring stations at Masaya and Telica, and spatial series data from Masaya, Telica, …

Transendothelial Migration Of Metastatic Cancer Under The Influence Of Cigarette Smoke Condensate, Daniel Opp

USF Tampa Graduate Theses and Dissertations

Cigarette smoke's influence on cancer has primarily been a subject of epidemilogic and tumorigenic studies. There have been no proper investigations with interests focused on how cigarette smoke affects the cellular mechanics of metastasis. Gathering an understanding of how smoke influences metastatic invasion could be vital in regulating or possibly eliminatings cancer's ability to initiate new tumor growth sites. This project focuses on cigarette smoke's influence on cellular mechanics of endothelial cells, and the invasive potential of cancer against a fully active endothelium. It is already known that cigarette smoke has a carcinogenic effect, but it is hypothesized that the …

Operational And Safety Effects Of Signage And Lighting Configurations For Public Transit Buses In Florida, Stephanie Antoinette Bromfield

USF Tampa Graduate Theses and Dissertations

Although public transit bus accounts for only a small percentage of the mode share for transportation in Florida, the annual passenger miles were over 1 billion with over 200 million passenger trips in 2005. These numbers warrant close attention to be paid to the safety of public transit vehicles. Despite the relatively low occurrence of fatalities and bus crashes, each crash of a high occupancy vehicle such as a public transit bus could expose more people to injury than a private automobile crash. Bus crashes also have a significant impact on the automobiles that are involved. Since a high percentage …

Prediction Of English And Spanish Early Literacy Skills Of English Language Learners In The Primary Grades, Giselle Sanchez

USF Tampa Graduate Theses and Dissertations

This study explored how language, emergent literacy, and reading skills in both English and Spanish develop with a group of English language learners (ELLs) (n = 267). Specifically, the researcher investigated what early language and literacy skills were the most important predictors of reading abilities as indicated by the Book Task in prekindergarten through first grade. Early language and literacy skills were assessed utilizing subtest from the Woodcock Language Proficiency Batter - Revised, the Woodcock Language Proficiency Battery - Revised - Spanish Form and the Phonological Awareness Task. Participants came from households where Spanish was one of the languages spoken. …

Wheelchair Positioning And Pulmonary Function In Children With Cerebral Palsy, Lee Barks

USF Tampa Graduate Theses and Dissertations

Background: In children with cerebral palsy (CP), poor trunk control fosters spinal deformity, pulmonary compromise (Canet, et al., 1998), increased health risks, and costs of long-term care (Braddock, 2001). Evidence links posture and pulmonary function, but influence of wheelchair parameters on pulmonary mechanics is unknown.

Objectives: 1) Determine relative contribution of five wheelchair configuration parameters to improvement in pulmonary mechanics--total airway resistance (RAW), tidal volume, minute ventilation (MV), and deadspace to tidal volume ratio; 2) Describe recruitment and retention of school-aged children with CP; and 3) Discuss response of the participants to the protocol.

Method: This within-subjects, descriptive study employed …

The Relationships Among Pain, Dyspnea, Constipation And Quality Of Life In Lung Cancer Patients Enrolled In A Hospice Program, Lisa A. Laches

USF Tampa Graduate Theses and Dissertations

There is evidence of a relationship between pain and associated symptoms, specifically constipation and dyspnea, and quality of life. Literature supports that endstage lung cancer patients suffer more symptoms than those with other types of cancers, and the course of treatment is primarily palliative, as many of these diagnosed patients cannot be cured. The purpose of this secondary analysis of data was to evaluate the relationships between pain and other common symptoms in end stage lung cancer patients in hospice care, and the relationships among pain, dyspnea, constipation and quality of life. The study sample included fifty lung cancer patients …

Effects Of Dual Language Learning On Early Language And Literacy Skills In Low Income Preschool Students, Vanessa Tápanes

USF Tampa Graduate Theses and Dissertations

This paper presents a framework for literacy skill development relating to both monolingual and dual language learners. The purpose of this study was to identify the differences that may exist between monolingual and dual language learners' performance on literacy tasks, before having a significant amount of exposure to the preschool curriculum. The sample included 78 monolingual language learners and 44 dual language learners who were assessed using the Woodcock Language Proficiency Battery-Revised (WLPB-R). The researcher used scoring methods that took into consideration split vocabulary in dual language learners where a conceptual scoring technique was used (Bedore, Pena, Garcia, & Cortez, …

Perceptions Of School Based Mental Health Services By Directors And Supervisors Of Student Services, Decia N. Dixon

USF Tampa Graduate Theses and Dissertations

Key stakeholders in schools must be educated about the importance of increasing access to mental health services in schools. School-based mental health services are designed to increase children's competence and help them meet the societal expectations of school success. The present study examined types of mental health services provided to students in school districts throughout Florida; the extent to which those services were provided to children and families; the beliefs of student services directors and supervisors regarding qualifications of school mental health service providers to provide mental health services; and their beliefs about the impact of mental health services on …

Cellmap: An Automated Multielectrode Array Cell Culture Analysis System Based On Electrochemical Impedance Spectroscopy, Abdur Rub Abdur Rahman

USF Tampa Graduate Theses and Dissertations

The objective of this research is to develop fundamental understanding of cell-substrate (CS) and cell-cell (CC) interactions in the culture space for time evolving cell cultures. Space resolved CC and CS interactions are important indicators of cell-density distribution, localized cellular behavior, and multiple cell-layers which are differentiators of normal and abnormal cell behavior. In this research, CS and CC interactions and the variations therein due to a) Cell growth, 2) cell-drug interaction, and 3) effect of Cytotoxin were studied using multielectrode, multi-frequency Electrochemical Impedance Spectroscopy (EIS). Contemporary impedance based methods sense either CC or CS interaction as a space averaged …

Hydrostratigraphy And Groundwater Migration Within Surficial Deposits At The North Lakes Wetland, Hillsborough County, Florida, Jason J. Laroche

USF Tampa Graduate Theses and Dissertations

A wetland in west-central Florida was studied to characterize the local hydrostratigraphic configuration of surficial deposits overlying more-permeable limestones and conceptualize groundwater recharge. Eight continuous cores were drilled through the surficial deposits and partially into the underlying limestone. A total of 111 samples were extracted from the cores for laboratory sediment analyses and testing. The surficial deposits are roughly eight meters thick and made up of upper and lower clean-sand hydrostratigraphic layers (S1 and S3, respectively) separated by a low-permeability layer of clayey sand (S2). Also, a discontinuous low-permeability layer of clayey sand (S4) lies between S3 and the top …

An Urban Model Of Applied Preservation, David Stewart Barksdale Butler

USF Tampa Graduate Theses and Dissertations

This research prioritized the identification and retention of African American cultural heritage in the face of dramatic landscape alteration associated with comprehensive redevelopment. As an approach aimed at providing the most comprehensive understanding of cultural phenomenon, the holistic tradition applied by anthropology asserts that it is productive to identify and apply as many sources of data toward engaging research as is possible. Consistent with this goal, this study applied several categories of data toward investigating material symbols of African American cultural heritage in Tampa, Florida. The holistic anthropological approach demonstrated the relevance and complementarity of research documenting cultural heritage and …

Electrochemical Behavior Of Aluminized Steel Type 2 In Scale-Forming Waters, Leonardo Caseres

USF Tampa Graduate Theses and Dissertations

Aluminized steel Type 2 (AST2), often used for culvert pipes, is subject to corrosion which is the most important durability limitation factor. It was desired to determine if the outer aluminized layer will retain passivity and if protective galvanic action will develop. Thus, corrosion of unblemished and blemished AST2 surfaces was investigated in simulated natural waters.

Experiments with unblemished specimens showed passive corrosion rates (~0.06 µm/yr) in scale-forming, 0.01 M Cl- solutions but sustained corrosion in other less protective media (with rates 3~10 µm/yr). Corrosion was manifested macroscopically by discoloration and few macro pits, but it likely proceeded also microscopically …