Arts and Humanities Commons^™

B-Raf Is An Essential Component Of The Mitotic Machinery Critical For Activation Of Mapk Signaling During Mitosis In Xenopus Egg Extracts, Sergiy I. Borysov

USF Tampa Graduate Theses and Dissertations

Activation of the MAPK cascade during mitosis is critical for spindle assembly and normal mitotic progression. The underlying regulatory mechanisms that control activation of the MEK/MAPK cascade during mitosis are poorly understood. The goal of my dissertation research is to identify the MEK kinase responsible for activation of the MAPK cascade during mitosis and to elucidate the biochemical mechanisms that regulate its activity. In the described herein work I purified and characterized the MEK kinase activity present in M-phase arrested Xenopus egg extracts. I demonstrate that B-Raf is the critical MEK kinase required for activation of the MAPK pathway at …

Statistical Modeling And Assessment Of Software Reliability, Louis Richard Camara

USF Tampa Graduate Theses and Dissertations

The present study is concerned with developing some statistical models to evaluate and analyze software reliability. We have developed the analytical structure of the logistic model to be used for testing and evaluating the reliability of a software package. The proposed model has been shown to be useful in the testing and debugging stages of the developmental process of a software package. It is important that prior to releasing a software package to marketing that we have achieved a target reliability with an acceptable degree of confidence. The proposed model has been evaluated and compared with several existing statistical models …

Enzyme Linked Spectroscopic Assays For Glyoxylate; The Use Of Peptidylglycine Alpha-Amidating Monoxygenase For The Discovery Of Novel Alpha-Amidated Hormones, Sarah Elizabeth Carpenter

USF Tampa Graduate Theses and Dissertations

Peptide hormones are responsible for cellular functions critical to the survival of an organism. Approximately 50% of all known peptide hormones are post-translationally modified at the C-terminus. Enzymatic oxidative conversion of C-terminal glycine extended peptide precursors results in an a-amidated peptide and glyoxylate. Peptidylglycine a-amidating monooxygenase (PAM) is the single known enzyme responsible for catalyzing this reaction. PAM is an O2, Cu(II), and Zn(II) dependent bifunctional enzyme. Initially, PAM hydroxylates the glycyl a-carbon followed by dealkylation of the hydroxylated intermediate to an a-amidated product and glyoxylate. PAM is also responsible for the conversion of glycine extended fatty acids to fatty …

Stabilization Of Marginal Soils Using Recycled Materials, Delfin G. Carreon

USF Tampa Graduate Theses and Dissertations

Marginal soils, including loose sands, soft clays, and organics are not adequate materials for construction projects. These marginal soils do not possess valuable physical properties for construction applications. The current methods for remediation of these weak soils such as stone columns, vibro-compaction, etc. are typically expensive. Waste materials such as scrap tires, ash, and wastewater sludge, offer a cheaper method for stabilizing marginal soils. As an added benefit, utilizing waste materials in soil stabilization applications keeps these materials from being dumped into landfills, thereby saving already depleting landfill space. Included in this report is an extensive investigation into the current …

Cloning And Analysis Of Putative Collagenases Of The U32 Family In Streptococcus Mutans And Streptococcus Agalactiae (Group B Streptococci), Valerie Carson

USF Tampa Graduate Theses and Dissertations

Analysis of the genomic sequences of Streptococcus mutans UA159 and Group B Streptococcus (GBS) strains Streptococcus agalactiae NEM316 and S. agalactiae 2603V/R indicated the presence of two putative collagenase genes in each organism. smcol1 from S. mutans was previously cloned and analyzed and the results indicated that the enzyme belonged to the U32 family of collagenases/peptidases. This enzyme shares homology with the prtC of Porphyromonas gingivalis, one of the principal examples of the U32 family of peptidases. Considering the potential role of these enzymes in the pathogenicity of P. gingivalis (periodontitis or gum disease), GBS (premature rupture of the amniochorionic …

Cue Reactivity And The Role Of Social Alcohol Expectancies In The College-Aged Drinking Population, Ashlee C. Carter

USF Tampa Graduate Theses and Dissertations

Research has shown alcohol expectancies to be critically important in understanding maladaptive drinking patterns within alcohol use disorders. Alcohol expectancies, thought to be automatically elicited in the presence of environmental alcohol-related cues, represent both cognitive and affective associations with drinking behavior. However, the automatic and affective properties of alcohol expectancies have not yet been thoroughly measured in the literature. Psychophysiological measures, including skin conductance, heart rate, and the acoustic startle response in particular, offer a uniquely powerful set of indices for the automatic affective processing of alcohol-related cues.

Therefore, the present study was designed to examine how alcohol expectancies moderate …

The Hearing Abilities Of Elasmobranch Fishes, Brandon M. Casper

USF Tampa Graduate Theses and Dissertations

The hearing abilities of elasmobranch fishes were examined in response to several types of stimuli using auditory evoked potentials (AEP). Audiograms were acquired for the nurse shark, Ginglymostoma cirratum, the yellow stingray, Urobatis jamaicensis, in a controlled environment using a monopole underwater speaker. A dipole stimulus was used to measure the hearing thresholds of the horn shark, Heterodontus francisi, and the white-spotted bamboo shark, Chiloscyllium plagiosum. The dipole experiments yielded much lower thresholds than any other experiment, suggesting that this type of sound specifically stimulated the macula neglecta by creating a strong velocity flow above the head of the shark. …

Folk Wiki: The Shared Traditions Of Folk Music And The Wiki Way, Phillip Mark Chamberlin

USF Tampa Graduate Theses and Dissertations

Wiki is often perceived as representing a revolutionary break from conventional notions of authorship, writing, and textual history. Dialogues concerning Wiki tend to ignore the characteristics that Wiki shares with earlier forms of collaboration, particularly folk music. In both Wiki and folk music, content is often collectively shared and authored, even if specific individuals create and change the content. Many collaborators are anonymous, quasi-anonymous, or pseudo-anonymous, but the perception of this anonymity is, in both genres, problematic. Second, both Wiki documents and folk songs exist in the "Eternal Now," a seemingly perpetual state that makes these texts available for addition, …

The Effects Of Multimedia Annotations On L2 Vocabulary Immediate Recall And Reading Comprehension: A Comparative Study Of Text-Picture And Audio-Picture Annotations Under Incidental And Intentional Learning Conditions, Zhaohui Chen

USF Tampa Graduate Theses and Dissertations

This dissertation investigated the effects of multimedia annotation on L2 vocabulary learning and reading comprehension. The overarching objective of this study was to compare the effects of text-picture annotation and audio-picture annotation on L2 vocabulary immediate recall and reading comprehension. This study also sought to examine the different effects under incidental and intentional learning conditions. The participants were 78 intermediate adult ESL learners from three universities in northwest U.S. The participants read an Internet-based English text. Twenty target words, annotated in either text-picture or audio-picture, were embedded in the reading text. The participants accessed the annotations by clicking on the …

Monitoring Water Quality In Tampa Bay: Coupling In Situ And Remote Sensing, Zhiqiang Chen

USF Tampa Graduate Theses and Dissertations

Water quality in Tampa Bay was examined using concurrent in situ and satellite remote sensing observations. Chlorophyll and suspended sediment concentrations showed large short-term variability, primarily driven by tide and wind forcing. Superimposed on these high frequency variations were recurrent phytoplankton blooms stimulated by decreases in turbidity 1-2 days after wind-induced bottom sediment resuspension events; the blooms were particularly strong if neap tides occurred after the wind events. The in situ data show that observations once per month are inadequate to sample short-term variability and that therefore the current monthly water quality surveys may have uncertainties of -50 to 200% …

Genetic Variation In The Chloroplast Genome Of A Newly Described Aster Species, Chrysopsis Delaneyi, Justine Ann Clark

USF Tampa Graduate Theses and Dissertations

The genus Chrysopsis (Asteraceae) contains eleven species native to Florida, including the newly described species, Chrysopsis delaneyi. Populations of this endemic plant species inhabit the Lake Wales Ridge (LWR) and the Atlantic Ridge (AR) of the Florida peninsula. Differences in morphology have been demonstrated within C. delaneyi, based on their locations. My objective was to determine the relationships between the LWR and the AR populations by analysis of chloroplast sequence and nuclear sequence variation. Approximately 160 samples of C. delaneyi and its sister species C. scabrella have been collected from fifteen sites throughout Florida. Six single base differences were detected, …

Performance Based Design Of Degrading Structures, Mouchir Chenouda

USF Tampa Graduate Theses and Dissertations

Seismic code provisions are now adopting performance-based methodologies, where structures are designed to satisfy multiple performance objectives. Most codes rely on approximate methods to predict the desired seismic demand parameters. Most of these methods are based on simple SDOF models, and do not take into account neither MDOF nor degradation effects, which are major factors influencing structural behavior under earthquake excitations. More importantly, most of these models can not predict collapse explicitly under severe seismic loads. This research presents a newly developed model that incorporates degradation effects into seismic analysis of structures. A new energy-based approach is used to define …

Traumatic Loss And Transformative Life Experiences: The Lived Experience Of Green Cross Traumatologists Deployed To The New York City World Trade Center Disaster, Carron C. Cherrie

USF Tampa Graduate Theses and Dissertations

This exploratory study examines the lived experience of Green Cross traumatologists deployed to the New York City World Trade Center disaster. The deployment took place five days after the terrorist attacks on September 11, 2001. The author was a member of a ten member advance team that provided crisis stabilization services to an international union in Lower Manhattan. Disaster Mental Health Services and community outreach were provided for a month. The purpose of the study was to describe in an anthropologically holistic perspective the lived experience of traumatologists, who as Americans were also affected by the terrorist disaster. Thirty-one traumatologists …

The Influence Of Performance Background On Instrumentalists’ Ability To Discriminate And Label Cornet And Trumpet Timbre, Gary Compton

USF Tampa Graduate Theses and Dissertations

This study was an examination of timbre discrimination and labeling, with specific consideration of the soprano-brass timbres of cornets and trumpets. This study sought to determine whether instrumentalists differed in their ability to label and discriminate cornet and trumpet timbres, and whether these abilities were influenced by the instrumentalists' performing instrument and years of playing experience. Wind, brass and percussion players (n=100) from existing instrumental groups in the Tampa Bay area participated in the study. These groups were collegiate trumpet majors from a large public university school of music, a collegiate wind ensemble from a private university, and a brass …

Behavioral And Auditory Evoked Potential (Aep) Hearing Measurements In Odontocete Cetaceans, Mandy Lee Hill Cook

USF Tampa Graduate Theses and Dissertations

Bottlenose dolphins (Tursiops truncatus) and other odontocete cetaceans rely on sound for communication, navigation, and foraging. Therefore, hearing is one of their primary sensory modalities. Both natural and anthropogenic noise in the marine environment could mask the ability of free-ranging dolphins to detect sounds, and chronic noise exposure could cause permanent hearing losses. In addition, several mass strandings of odontocete cetaceans, especially beaked whales, have been correlated with military exercises involving mid-frequency sonar, highlighting unknowns regarding hearing sensitivity in these animals.Auditory evoked potential (AEP) methods are attractive over traditional behavioral methods for measuring the hearing of marine mammals because they …

The Association Of Head Circumference With Selected Cognitive Outcomes In Older Adults In Charlotte County, Florida, Cathleen Copenhaver

USF Tampa Graduate Theses and Dissertations

OBJECTIVE: The brain reserve hypothesis was examined in a secondary analysis of cross-sectional data from a community-based sample of 468 older adults residing in Charlotte County, Florida. The objective of the analysis was to determine the association between head circumference and eight cognitive outcomes and to assess any potential effect modification of existing associations by Apolipoprotein E (APOE) genotype. METHODS: Cognitive outcomes include scores from the Modified Mini-Mental State Exam (3MS), the Hopkins Verbal Learning Test-Revised (HVLT-R), Stroop Color-Word Test, Trail-Making Test A and B, and a word-stem completion task measuring implicit memory. Descriptive statistics were calculated for each variable. …

A Descriptive Analysis Of The Relationship Between Specific Teacher Characteristics And Teacher Efficacy In Florida’S Low-Performing Public High Schools, Pamela S. Craig

USF Tampa Graduate Theses and Dissertations

This study was designed to collect data to determine the specific characteristics (gender, level and area of degree status, certification status, pedagogical training, gender, number of years of teaching experience, number of years teaching at the current school, and courses currently taught) of language arts teachers at Florida's low-performing pubic high schools and compare these characteristics to teachers' sense of efficacy (the extent to which teachers' believe they have the ability to bring about changes in student achievement independent of the student's background, behaviors, or motivation level). A total of 615 teachers representing 84 schools in 36 districts participated in …

Risk And Protective Factors Related To Resilience In Adolescents In An Alternative Education Program, Kelly M. Crawford

USF Tampa Graduate Theses and Dissertations

Using a multivariate correlational design, this study was designed to determine the degree to which selected risk and protective factors were related to resilience in an at-risk student population. A total of 12 individual, family and external risk and protective factors were identified and data were collected from 142 6th through 11th grade students in an alternative education program for individuals who had committed severe behavioral infractions. A series of multiple regression analyses were conducted using three outcome variables: a rating of students' resilient behavior, number of behavior referrals in the students' records, and standardized reading achievement scores. Five percent …

Some Problems On Products Of Random Matrices, Edgardo S. Cureg

USF Tampa Graduate Theses and Dissertations

We consider three problems in this dissertation, all under the unifying theme of random matrix products. The first and second problems are concerned with weak convergence in stochastic matrices and circulant matrices, respectively, and the third is concerned with the numerical calculation of the Lyapunov exponent associated with some random Fibonacci sequences. Stochastic matrices are nonnegative matrices whose row sums are all equal to 1. They are most commonly encountered as transition matrices of Markov chains. Circulant matrices, on the other hand, are matrices where each row after the first is just the previous row cyclically shifted to the right …

The Identification Of Functional, Sequestered, Symbiotic Chloroplasts In Elysia Clarki: A Crucial Step In The Study Of Horizontally Transferred, Nuclear Algal Genes, Nicholas E. Curtis

USF Tampa Graduate Theses and Dissertations

A comparison of Elysia (=Tridachia) crispata (Mörch, 1863) from the Virgin Islands with elysiid slugs from the mangrove swamps and canals in the Florida Keys that have previously been identified as E. crispata reveals many differences in habitat, gross and microscopic anatomy, food preferences of juveniles, sources of symbiotic chloroplasts and their localization within the digestive tubules, radular morphology, and nucleic acid sequences of two genes. The differences between the two groups of slugs are such that the Florida Keys animals are considered to represent a new species, Elysia clarki. Elysia clarki feeds on siphonaceous algae, and intracellularly sequesters the …

Parent Involvement: Differences Between African Americans And European Americans In One Florida School District, Michelle Darter-Lagos

USF Tampa Graduate Theses and Dissertations

The purpose of this research study was to (a) analyze perceptions of parent involvement across raters (i.e., seventh grade students versus their parents) and across ethnicity (i.e., African American versus European American), and (b) examine how perceptions of parent involvement are related to academic achievement. A subsample of archival survey data collected in one central Florida school district was analyzed for the current study. Findings revealed a positive but weak relationship between students' and parents' perceptions of parent involvement regardless of ethnicity. Significant differences were found in the perceived levels of involvement by ethnicity, even when controlling for SES. In …

Pain As A Motivator Of Smoking: Effects Of Pain Induction On Smoking Urge And Behavior, Joseph W. Ditre

USF Tampa Graduate Theses and Dissertations

Tobacco smoking has been associated with the development, protraction, and exacerbation of chronically painful conditions. Conversely, there is reason to believe that smokers may be motivated to use tobacco as a means of coping with their pain. To date, no controlled, experimental studies have tested for a causal relationship between pain and smoking motivation. The primary aim of the current study was to test the hypothesis that laboratory-induced cold-pressor pain would enhance smoking motivation, as measured by self-reported urge to smoke and observation of immediate smoking behavior. The effect of a smoking cue was also tested. Smokers (n = 132) …

Charaterization Of Asbestos Exposure Among Automotive Mechanics Servicing And Handling Asbestos-Containing Materials, Gary Scott Dotson

USF Tampa Graduate Theses and Dissertations

The historic use of asbestos-containing materials during the manufacturing of automobiles has resulted in a perception of an increased risk of asbestos-related pulmonary diseases within mechanics. This study was conducted to assess the potential asbestos exposures mechanics encounter while servicing vehicles assembled with parts containing asbestos, in addition to compare the cumulative lifetime asbestos exposures for different maintenance activities against theorical threshold exposures for asbestosis, lung cancer and mesothelioma. Exposure data were assembled from four independent exposure assessments performed to elucidate the airborne asbestos levels generated during the removal and replacement of brakes, gaskets, clutches and seam sealants containing asbestos. …

The Impacts Of The Handoffs On Software Development: A Cost Estimation Model, Michael Jay Douglas

USF Tampa Graduate Theses and Dissertations

Effective software cost estimation is one of the most challenging and important activities in software development. The software industry does not estimate projects well. Poor estimation leads to poor project planning with resulting schedule overruns, inadequate staffing, low system quality, and many aborted projects. Research on software estimation is needed to build more accurate models of the key aspects of software development. The goals of research in this dissertation are to investigate and improve the modeling of team size and project structures in current software estimation methods.Mathematical models for estimating the impacts of project team size and three variations of …

Evaluation Of Certain Exponential Sums Of Quadratic Functions Over A Finite Field Of Odd Characteristic, Sandra D. Draper

USF Tampa Graduate Theses and Dissertations

Let p be an odd prime, and define f(x) as follows: f(x) as the sum from 1 to k of a_i times x raised to the power of (p to the power of (alpha_i+1)) in F_(p to the power of n)[x] where 0 is less than or equal to alpha_1 < alpha_2 < ... < alpha_k where alpha_k is equal to alpha. We consider the exponential sum S(f, n) equal to the sum_(x as x runs over the finite field with (p to the n elements) of zeta_(p to the power of Tr_n (f(x))), where zeta_p equals e to the power of (2i times pi divided by p) and Tr_n is the trace from the finite field with p to the n elements to the finite field with p elements.We provide necessary background from number theory and review the basic facts about quadratic forms over a finite field with p elements through both the multivariable and single variable approach. Our main objective is to compute S(f, n) explicitly. The sum S(f, n) is determined by two quantities: the nullity and the type of the quadratic form Tr_n (f(x)).

We give an effective algorithm for the computation of the nullity. Tables of numerical values of the nullity are included. However, the type is more subtle and more difficult to determine. Most of our investigation concerns the type. We obtain "relative formulas" for S(f, mn) in terms of S(f, n) …

Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon

USF Tampa Graduate Theses and Dissertations

Streptococcus pneumoniae is a major bacterial pathogen which causes pneumoniae, meningitis, otitis media, and bacteremia. Currently there are two vaccines available, Pneumococcal Polysaccharide Vaccine (PPV) for adults and the Pneumococcal Conjugate Vaccine (PCV) for children. The PCV vaccine was developed in 2000 specifically for children and infants due to the ineffectiveness of the PPV vaccine in children. This is a cross sectional study of invasive S. pneumoniae in Florida during 2003. This study is designed to determine the population characteristics, clinically relevant antibiotic resistance patterns and specific risk factors for development of antibiotic resistance of invasive S. pneumoniae. Participants for …

Optical Imaging Of Radiolabeled Drugs In Tissue Sections Using The Microimager, Paul Dungel

USF Tampa Graduate Theses and Dissertations

The MicroImager is a fast, high resolution, real time, digital autoradiographic imaging tool with broad applications. This study utilizes the MicroImager to evaluate radiolabeled drug behavior in subcutaneous tissue. Experiments were conducted in conjunction with mathematical models to determine the diffusion coefficient (D) and elimination constant (k) for radiolabeled dexamethasone. Osmotic pumps containing [3H]dexamethasone were implanted into rat subcutaneous tissue over 6h, 24 h, and 60 h. Local tissue was explanted and slides were prepared for imaging. The MicroImager was then used to quantify the local concentration of 3H-dexamethasone in the tissue surrounding the tip of the osmotic pump. Betavision+ …

Vibration Level Characterization From A Needle Gun Used On U.S. Naval Vessels, Scott E. Dunn

USF Tampa Graduate Theses and Dissertations

United States (U.S.) Navy sailors are exposed to a very large number of hazards, both chemical and physical. Occupational vibration from pneumatic air tools is one of the potential exposure hazards. There are very limited data as to the exposures to one type of tool, a needle gun or needle scaler, used by the sailors.The purpose of this study was to characterize the vibration levels generated by a needle gun used in the U.S. Navy. The design of the study evaluated the difference pressure had on the acceleration levels generated from the needle scaler. Five subjects were used in the …

Improving Long-Term Resettlement Services For Refugees, Asylees, And Asylum-Seekers: Perspectives From Service Providers, Kristina M. Dunman

USF Tampa Graduate Theses and Dissertations

In the United States, rights of asylum and refuge are extended to people who can prove that they have been politically persecuted. Resettlement services for refugees and asylees often focus on the short-term acquisition of employment and English language skills. These policies ignore the long-term complexities involved in reestablishing individual and group identities after war trauma and resettlement. This research is an investigation into the perspectives of service providers who work with refugees, asylees, and asylum-seekers on the need for more comprehensive, long-term services to assist in the months and years following resettlement, and on potential programs to address those …

Monitoring And Modeling Water Quality At The C.W. Young Regional Reservoir, Daniel Robert Dye

USF Tampa Graduate Theses and Dissertations

This work explores the relationship between nutrient loading and changes in water quality in a sub-tropical, above-ground, off-stream municipal water supply reservoir, the C.W. Bill Young Regional Reservoir. The three source waters for the reservoir have varied but high levels of nutrients such as phosphorus and nitrogen. In other reservoirs, these nutrients have been linked to deterioration of water quality and increased expense in water treatment. The need to minimize excess nutrients results led to the primary research question: what allocation of withdrawals from the three sources will minimize the deterioration of water quality? To answer this question, the relationship …