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Articles 51151 - 51180 of 1824506
Full-Text Articles in Entire DC Network
Pronatalism In The United States: How Political Ideals Surrounding Family And Reproductive Health Care Contribute To Neglect In The Foster Care System, Carson Sebree
Undergraduate Honors Theses
Within the past decade, conservative reproductive politics in the United States have experienced an ideological shift from pro-life toward pronatalism. This has been intensified through the rollback of reproductive rights and the rise of Christian nationalism. However, there has been no commensurate additional funding for the foster care system. This project asks the interrelated questions of: “how is pronatalism linked to neglect within the foster care system?; and, how do theories of democratic care highlight the necessity to rethink support for the foster care system and create policies that will address its shortcomings, given the rise of pronatalist aw and …
Eyetracking Measures Of Performance On The Traveling Salesperson Problem, Kanan Levy
Eyetracking Measures Of Performance On The Traveling Salesperson Problem, Kanan Levy
Undergraduate Honors Theses
Human solutions to the Traveling Salespersons Problem (TSP) have been proposed to employ heuristics integrating global and local spatial information (Pizlo et al., 2006). Because different neuroanatomical regions may be involved in local vs. global processing, as well as attentional shift between levels, performance on the TSP may provide useful insight into changes that occur in the brain as a result of age or of neurodegenerative disorders (Slavin, 2002). In a previous study, we compared the cognitive processes used to solve spatial problems on a large scale (moving through space) and on a small scale (on a sheet of paper). …
The Gray Bird Sings: The Extraordinary Life Of Betty Kwan Chinn (Second Edition), Karen M. Price Ph.D.
The Gray Bird Sings: The Extraordinary Life Of Betty Kwan Chinn (Second Edition), Karen M. Price Ph.D.
Trade & Scholarly Monographs
“Touched by childhood tragedy, Betty Chinn brings hope to those who have fallen on hard times. Left homeless as a child in China, Chinn became mute. When she came to America, she found both her voice and her mission: aiding those without shelter on our own shores. ” -Barack Obama, awarding Betty the Presidential Citizens Medal
As a child during the Chinese Cultural Revolution, she was separated from her family, imprisoned, and ruthlessly tortured. As an immigrant to the United States, she worked tirelessly to shelter and feed thousands in her community. How she survived monstrous conditions, challenged impossible odds, …
Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon
Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon
Honors Scholar Theses
Global demand for animal protein is increasing rapidly, underscoring the need for sustainable meat production. Cultured meat is a promising alternative to traditional meat production techniques, but it requires optimization before it can be implemented on a large scale. Scaffolds are an important component for making cultured meat, as they provide structural support for cell attachment and mimic the texture of meat. Scaffolds need to be biocompatible, mechanically supportive, and biochemically active to promote proper cell adhesion, proliferation, and differentiation. This project investigated brown seaweed extract (SWE) hydrogels as a scaffold for cultivated meat applications and compared their mechanical and …
Teaming With Insects: Entomology For Grades 3-12, Natalie J. Carroll, Christian Y. Oseto, Timothy J. Gibb
Teaming With Insects: Entomology For Grades 3-12, Natalie J. Carroll, Christian Y. Oseto, Timothy J. Gibb
Purdue University Press Books
Teaming With Insects: Entomology for Grades 3–12 is written for youth who enjoy learning about and studying science and nature. Insects have been around for millions of years and have evolved and adapted to many different habitats. Adaptations involving their anatomy, physiology, and behavior are what make insects so diverse and interesting. More than 1 million species of insects are known to scientists, and they believe there may be up to 40 million more we have yet to discover. Most people consider insects pests, and some insects do damage to homes, plants, crops, and stored foods. Others harm humans and …
A Shattered World: Jews And Israel After October 7, Michael Berenbaum, Gilbert N. Kahn
A Shattered World: Jews And Israel After October 7, Michael Berenbaum, Gilbert N. Kahn
The Jewish Role in American Life: An Annual Review
October 7, 2023, was a watershed event in contemporary Jewish history. What took place on that date challenged the two most basic assumptions of post-Holocaust Jewish life: that the future security of the Jewish people would be assured by an independent Jewish state backed by an army, and that the freedom for Jews in the Diaspora to live as Jews would be secured by democratic rights and liberties. The pogroms of October 7 and the worldwide explosion of antisemitism that followed—even preceding Israel taking military action against Hamas—challenged both assumptions. The Israel Defense Forces could not prevent a slaughter from …
Intervention Outcomes Of A Family-Based Intervention: A Case Review Of An Asian American Adolescent At Clinical High Risk For Psychosis, Monica Elizabeth Done
Intervention Outcomes Of A Family-Based Intervention: A Case Review Of An Asian American Adolescent At Clinical High Risk For Psychosis, Monica Elizabeth Done
UNLV Theses, Dissertations, Professional Papers, and Capstones
Schizophrenia is a lifelong and debilitating condition that occurs in approximately 1% of individuals globally and includes positive, negative, disorganized, and other symptoms that interfere with functioning. The onset typically happens in adolescence, and recently, there has been an emphasis on finding individuals who are at high clinical risk for developing psychosis (CHR-P). Additionally, there has been a focus on creating intervention programs that may treat symptoms in the early phases of illness and decrease the likelihood of developing a schizophrenia spectrum and other psychotic disorders (SSD). Furthermore, there is limited research on the potential impacts of cultural identity on …
Ankimedbench: Evaluating Hierarchical Medical Knowledge In Language Model Embeddings, Neel Patel
Ankimedbench: Evaluating Hierarchical Medical Knowledge In Language Model Embeddings, Neel Patel
UNLV Theses, Dissertations, Professional Papers, and Capstones
Despite achieving over 90% accuracy on medical benchmarks, recent studies show physicians cannot effectively leverage language models to improve clinical reasoning. Current benchmarks test isolated factual recall, but clinical practice requires hierarchical navigation through diagnostic categories—starting broad and narrowing systematically from chest pain to cardiovascular pathology to myocardial infarction to specific STEMI types. Existing evaluations cannot measure whether models preserve this taxonomic structure essential for clinical reasoning.
We introduce AnkiMedBench, built from 16,512 medical flashcards used by students preparing for licensing exams. Cards are organized across six hierarchy levels spanning 16 broad medical specialties to 672 specific diseases and conditions. …
A Competency Based Pressure Injury Management Program, Madina Qadir
A Competency Based Pressure Injury Management Program, Madina Qadir
UNLV Theses, Dissertations, Professional Papers, and Capstones
This project aims to implement a simulation-based wound management program to address critical gaps in nurses’ competencies for managing pressure injuries (PIs). The initiative seeks to enhance nurses’ knowledge, clinical skills, and confidence in wound assessment and advanced therapies, fostering evidence-based practice and consistent care delivery. Effective PI management is vital for improving patient outcomes, reducing healing times, and minimizing complications. Yet, nursing education and competency gaps often lead to inconsistent care and increased healthcare costs. A structured competency-based pressure injury management (CB PIM) program integrates evidence-based didactic lectures and interactive simulation-based skills stations to cover essential topics, including wound …
“I Can Gossip With Anybody, I Can’T Chisme With Everybody”: Chisme, Gossip, And Identity In Latina-Americans, Alexa B. Romero
“I Can Gossip With Anybody, I Can’T Chisme With Everybody”: Chisme, Gossip, And Identity In Latina-Americans, Alexa B. Romero
UNLV Theses, Dissertations, Professional Papers, and Capstones
As individuals adjust to their belonging to two distinct cultures, there are various ways of adapting to their navigation of the two cultures (Berry, 1980). The way(s) an individual chooses to adjust to their two cultures can have varying effects on identity. When directly translated, chisme (Spanish) means gossip. Although the two include overlap in function and uses, chisme offers unique purposes for the Latinx community and further, Latina-Americans (González Ybarra & Player 2024; Gutierrez, 2017; Uribe, 2025). By using the communication theory of identity (Hecht, 1993) the purpose of this thesis was to investigate the role of chisme and …
Las Voices: The Impact On Black Young Males In A Critical Mentorship Program In The Las Vegas Valley, Sean C. Tory
Las Voices: The Impact On Black Young Males In A Critical Mentorship Program In The Las Vegas Valley, Sean C. Tory
UNLV Theses, Dissertations, Professional Papers, and Capstones
In America, Black young males face an enormous amount of pressure regarding their social and racial identity. They battle with racial macro/microaggressions, stereotypes, racism, and socialization from various industries and systems. The community organization, King of Jewels, has been able to adopt effective strategies to help Black young males navigate these issues through critical mentorship. This paper will first demonstrate the importance of critical mentorship, Black Racial Identity Development, and will then outline the steps necessary to fill the gaps found in mentorship and Black Racial Identity Development literature. This qualitative study investigates the use of critical mentorship (CM) and …
Trauma Beyond Time: Temporal Constructs In Holocaust Testimonies, Sarah Seiselmyer-Snyder
Trauma Beyond Time: Temporal Constructs In Holocaust Testimonies, Sarah Seiselmyer-Snyder
Purdue University Press Books
Trauma Beyond Time: Temporal Constructs in Holocaust Testimonies challenges our understanding of what it means to be a Holocaust survivor, arguing that the term “post-Holocaust” fundamentally misrepresents survivors’ experiences. Through careful analysis of Holocaust literature and testimony, this book reveals how trauma persists across generations, defying conventional historical timelines. For those who perished, there can be no “after” to the Holocaust—their stories were violently ended. Yet for survivors, the Holocaust didn’t simply conclude in 1945. Their experiences demonstrate how trauma continues to shape lives decades later, making “post-Holocaust” a misleading concept that fails to capture their ongoing reality. Using the …
Building Your Epic Life: Your Journey, Your Way, Your Masterpiece, Luciano Castillo
Building Your Epic Life: Your Journey, Your Way, Your Masterpiece, Luciano Castillo
Purdue University Press Books
Building Your Epic Life empowers young people to understand that failure is an inevitable—and essential—part of the journey to success. The author shares his own challenges and struggles, showing that no path to success is linear and without setbacks. The book teaches the value of building a healthy body, mind, and spirit, and includes exercises that offer actionable plans for individuals to realize a meaningful, self-defined life based on their core values. A proven roadmap workbook and short lectures guide readers through a series of practical steps to discover their purpose, vision, and understand the value of mentorship—and why each …
Markets We Thought We Knew, Nicole J. Olynk Widmar, Michael L. Smith, Erin Robinson
Markets We Thought We Knew, Nicole J. Olynk Widmar, Michael L. Smith, Erin Robinson
Purdue University Press Books
Markets We Thought We Knew explores the evolving nature of markets and how they shape—and are shaped by—consumer behavior. From essential life necessities such as water to everyday commodities and products including locally sourced foods, this book covers a variety of topics and invites readers to examine a diverse range of markets through a reflective lens. By revisiting past market dynamics and real-world examples, readers are encouraged to rethink how preferences are formed, how opinions shift over time, and how value is assigned—sometimes in unexpected ways. What insights might you gather from a deeper understanding of local and global markets, …
Shattered Liberation: Sexualized Violence Against Holocaust Survivors, 1943–1946, Nina Paulovicova, Anna Cichopek-Gajraj, Joanna Beata Michlic
Shattered Liberation: Sexualized Violence Against Holocaust Survivors, 1943–1946, Nina Paulovicova, Anna Cichopek-Gajraj, Joanna Beata Michlic
Purdue University Press Books
Shattered Liberation: Sexualized Violence Against Holocaust Survivors, 1943–1946 challenges the notion of joyous liberation of Holocaust survivors by the Red Army, which is often embedded in popular imagination and collective memory. This is one of the first volumes to shine light on the sexualized violence that some Holocaust survivors endured in the hands of the Soviet Army, partisans, rescuers, and army personnel in filtration camps during the liberation process. By focusing on testimonies and memoirs, this book discusses in detail a wide range of interactions, including sexual violence, rape, forced cohabitation, sex barter, aid, and romance. The book addresses methodological …
Engaging Learners Everywhere: The Interactive Synchronous Hyflex Method, Nathan Mentzer, Abdul Moeed Asad, Adrie A. Koehler, Lakshmy Mohandas, Elnara Mammadova
Engaging Learners Everywhere: The Interactive Synchronous Hyflex Method, Nathan Mentzer, Abdul Moeed Asad, Adrie A. Koehler, Lakshmy Mohandas, Elnara Mammadova
Purdue University Press Books
Initially conceived as a workaround related to the crisis precipitated by the COVID-19 pandemic and the resulting lockdown of 2020, Interactive Synchronous HyFlex reimagines an active-learning, team-based classroom, making it possible for any student to participate fully, whether they are seated in the back row or joining from elsewhere in the world. Developed and refined by the authors through five years of classroom experimentation and National Science Foundation–funded research, this forward-facing approach—which refers to online synchronous hybrid delivery and combines the terms “hybrid” and “flexible”—brings together learners in a single classroom who have the daily option of meeting face-to-face or …
150 Years Of Purdue Engineering: Celebrating Our Consequential Impact On The World, Arvind Raman, Jim Small
150 Years Of Purdue Engineering: Celebrating Our Consequential Impact On The World, Arvind Raman, Jim Small
Purdue University Press Books
Since its founding 150 years ago, Purdue University’s College of Engineering has profoundly shaped and improved the world, amassing breakthroughs spanning nearly all facets of society. Highlighting the indomitable spirit, ingenuity, and achievements of more than 100 Boilermaker alumni and faculty members, 150 Years of Purdue Engineering describes the program’s journey to become one of the most consequential engineering colleges in the United States. Richly illustrated with archival and contemporary images, this book includes stories that focus on Purdue innovators who have created everything from the barcode and the electronic TV receiver to the soft-serve ice cream machine and artificial …
Soil And Water Education For Grades 3-12, Natalie J. Carroll, Christian Y. Oseto, Timothy J. Gibb
Soil And Water Education For Grades 3-12, Natalie J. Carroll, Christian Y. Oseto, Timothy J. Gibb
Purdue University Press Books
Soil and Water Education for Grades 3–12 was written for youth who enjoy learning about science and two critical natural resources: soil and water. Level 1 introduces basic terms and concepts, and is intended for youth in grades 3–5. Activities focus on understanding important soil and water processes. Designed for students in grades 6–8, Level 2 offers readers more concrete examples of advanced soil and water concepts. The exercises in Level 3, which is geared toward grades 9–12, are divided into sections based on how readers might use the information they have learned, placing them in the shoes of homeowners, …
Re: Approval Letter For The Revised Draft Final Annual Operations And Maintenance (O&M) Report: Butte Treatment Lagoon (Btl) System - 2023 (Dated October 29, 2025), Emma Rott
Silver Bow Creek/Butte Area Superfund Site
No abstract provided.
Teaching And Learning In Envisioning Byu, Volume 1: Foundations And Dreams: A Latter-Day Saint University Educative Perspective, Nicholas P. Bown
Teaching And Learning In Envisioning Byu, Volume 1: Foundations And Dreams: A Latter-Day Saint University Educative Perspective, Nicholas P. Bown
Undergraduate Honors Theses
This thesis outlines the principles of teaching and learning found within Envisioning BYU, Volume 1: Foundations and Dreams, a collection of foundational texts on education at Brigham Young University (BYU). In doing so, this thesis addresses a gap in internal university discourse about how to apply “gospel methodology” to teaching and learning at BYU. This study aims to elucidate these principles based on the assumption they may be of philosophical and practical value within BYU, as well as higher education more generally. In order to identify the salient teaching and learning principles found within Foundations, I conducted a qualitative textual …
Assessing Leadership In Business: A Critical Investigation Of Michelle Obama, Narineh Gevorkian
Assessing Leadership In Business: A Critical Investigation Of Michelle Obama, Narineh Gevorkian
Honors Scholar Theses
Michelle Obama, the former First Lady of the United States, has emerged as a powerful figure in politics, social advocacy, and public service. During her time in the White House, she worked on initiatives in education, health, and military family support while inspiring millions of people worldwide through her authenticity and advocacy. What distinguishes Michelle Obama as a leader is how her influence comes not from authority or hierarchy, but from connection, empathy, and moral purpose. This approach allows her to lead with authenticity, motivate others, and address inequalities within communities. As the first African American First Lady in US …
Cross-Country Differences In Adolescent Dietary Patterns And Body Mass Index: Spain And The United States, Rosa I. Chavez Jimenez
Cross-Country Differences In Adolescent Dietary Patterns And Body Mass Index: Spain And The United States, Rosa I. Chavez Jimenez
Honors Scholar Theses
In 2019, NCDs accounted for 38.8% of total global deaths in adolescents aged 10-24, and in 2024, the World Health Organization (WHO) reported that over 390 million youth were overweight or obese worldwide, highlighting the urgency of addressing poor nutrition and sedentary behaviors early in life. There is an extensive amount of research suggesting that the Mediterranean Diet (MD) is related to lowering NCDs and its risk factors. Thus, the present study explored cross-country differences in dietary patterns and Body Mass Index (BMI) between youth in Spain and the United States (US), two countries with contrasting dietary traditions: the Mediterranean …
Assessing Leadership In Business: A Critical Investigation Of Lorne Michaels, Emily Laput
Assessing Leadership In Business: A Critical Investigation Of Lorne Michaels, Emily Laput
Honors Scholar Theses
This thesis critically investigates the leadership effectiveness of Lorne Michaels, the executive producer behind the comedic institution Saturday Night Live (SNL). For nearly half a century, Michaels has cultivated a unique and high-pressure environment credited with launching the careers of numerous comedic actors. Drawing upon Kouzes & Posner's Five Principles of Exemplary Leadership framework and peer-reviewed research, this analysis examines the balance Michaels strikes between fostering innovation and exercising strict control. Ultimately, this thesis aims to provide a nuanced understanding of Lorne Michaels' leadership, assessing its effectiveness within the dynamic and often volatile environment of live television production and …
Stability Analysis Of Thermohaline Convection With A Time-Varying Shear Flow Using The Lyapunov Method, Kalin Kochnev
Stability Analysis Of Thermohaline Convection With A Time-Varying Shear Flow Using The Lyapunov Method, Kalin Kochnev
Honors Scholar Theses
This work applies the Lyapunov method to identify instabilities and compute the growth rate of a linear time-varying system. The linear system studied describes cold fresh water on top of hot salty water with a periodically time-varying background shear flow. A time-dependent weighting matrix is employed to construct a Lyapunov function candidate. The resulting linear matrix inequalities are discretized in time using the forward Euler method. As the number of temporal discretization points increases, the growth rate predicted by the Lyapunov method or Floquet theory, used for comparison, will converge to the same value obtained from numerical simulations. Furthermore, the …
Investigation Of Mono-Allelic Rack1 Deletion Effects On Cell Growth And Pkc Mrna Expression, Ashen Quiambao
Investigation Of Mono-Allelic Rack1 Deletion Effects On Cell Growth And Pkc Mrna Expression, Ashen Quiambao
Seton Hall University Dissertations and Theses (ETDs)
The µ-opioid receptor is a primary target of clinically used opioids, and RACK1 regulates its transcription by physically interacting with MOR transcription regulator PCBP1 in the cytosol to prevent its nucleus translocation. Two mono-allelic RACK1 knockout cell lines named RACKA and RACKB were generated by the CRISPR-Cas9 technique using WT NMB neuroblastoma cells. As RACK1 also contains a PKC-binding domain, this study aims to evaluate the impacts of mono-allelic deletion on cell growth rates and PKC isoform expression. Although a slight decreased tendency of growth rate in RACKA and RACKB cells was observed when compared to that of WT NMB …
Examining Aba Classroom Paraprofessional Training Methods In New Jersey, Melina Campos
Examining Aba Classroom Paraprofessional Training Methods In New Jersey, Melina Campos
Seton Hall University Dissertations and Theses (ETDs)
In recent years, paraprofessionals have become an integral part of educational teams, particularly in classrooms supporting students with disabilities (Giangreco et al., 2010). Their role has been crucial in ensuring that students received necessary academic and social support. This study evaluated the training provided to paraprofessionals in New Jersey, specifically those working in applied behavior analysis (ABA) classrooms. By surveying paraprofessionals on training that they receive and assessing how training influenced their self-perceived knowledge and skills, the study offered insight into how well-prepared these educators felt to support students with disabilities. A total of 57 participants completed the survey. When …
The Impact Of Youth Sports Participation On Academic Performance And Sense Of Belongingness In Middle School Students, Kevin Stahl
The Impact Of Youth Sports Participation On Academic Performance And Sense Of Belongingness In Middle School Students, Kevin Stahl
Seton Hall University Dissertations and Theses (ETDs)
This quantitative study explored the relationship between youth sports participation, academic achievement, and sense of belonging among middle school students. Using data from the Middle Grades Longitudinal Study of 2017–2018 (MGLS; National Center for Education Statistics [NCES], 2018), the research examined whether involvement in youth sports predicts outcomes in mathematics, reading, and students’ perceived connection to their school environment.
The findings challenged the extent to which Astin’s (1984) theory of student involvement generalizes to early adolescent populations. Although the theory proposes participation in structured activities supports both academic and social outcomes, results indicated stronger and more consistent associations between sports …
Integrating The Five As Framework To Enhance Person-Centered Care For Adults Living With Obesity, Alexandra Charles
Integrating The Five As Framework To Enhance Person-Centered Care For Adults Living With Obesity, Alexandra Charles
Electronic Theses and Dissertations 2020 - Present
Obesity has adverse effects on physical health, emotional well-being, and mental functioning. To effectively address this highly prevalent and multifaceted condition, the use of a structured, evidence-informed framework is essential to support providers in delivering consistent, person-centered care for individuals living with obesity. The Five As is a powerful yet not commonly implemented framework in many primary care practices. The Five As framework (Ask, Assess, Advise, Agree, Assist) offers a structured model to guide clinicians in delivering consistent, supportive, and transformative care for individuals with obesity.
The goal of the project was to implement a timely and cost-effective protocol/tool that …
Puf-Based Digital Money With Propagation-Of-Provenance And Offline Transfers Between Two Parties, Benjamin Bean, Cyrus Minwalla, Eirini Eleni Tsiropoulou, Jim Plusquellic
Puf-Based Digital Money With Propagation-Of-Provenance And Offline Transfers Between Two Parties, Benjamin Bean, Cyrus Minwalla, Eirini Eleni Tsiropoulou, Jim Plusquellic
Electrical and Computer Engineering ETDs
Building on prior concepts of electronic money (eCash), we introduce a digital currency where a physical unclonable function (PUF) engenders devices with the twin properties of being verifiably enrolled as a member of a legitimate set of eCash devices and possessing of a hardware-based root-of-trust. A hardware-obfuscated secure enclave (HOSE) is proposed as a means of enabling a PUF-based propagation-of-provenance (POP) mechanism, which allows eCash tokens (eCt) to be securely signed and validated by recipients without incurring any third party dependencies at transfer time. The POP scheme establishes a chain of custody starting with token creation, extending through multiple bilateral …
Benchmarking Of Duplex Sequencing Approaches To Reveal Somatic Mutation Landscapes, Yang Zhang, Vinayak V Viswanadham, Michail Andreopoulos, Dominik Glodzik, Ruolin Liu, Lovelace J Luquette, Se-Young Jo, Azeet Narayan, Muchun Niu, Lisa Anderson, Joseph A Brew, Hsu Chao, Carrie Cibulskis, Guanlan Dong, Uday S Evani, William C Feng, Marta Grońska-Pęski, Adrienne Helland, Nazia Hilal, Nisrine T Jabara, Hu Jin, Ning Li, Monica D Manam, Shayna L Mallett, Alexi Runnels, Constantijn Scharlee, Carter Smith, Smaht Duplex Sequencing Focus Group, Diane Shao, Christopher A Walsh, Viktor A Adalsteinsson, Eunjung Alice Lee, Peter J Park, Kristin G Ardlie, Soren Germer, Richard A Gibbs, Sangita Choudhury, Harsha V Doddapaneni, Gilad D Evrony, Chenghang Zong, Tim H H Coorens
Benchmarking Of Duplex Sequencing Approaches To Reveal Somatic Mutation Landscapes, Yang Zhang, Vinayak V Viswanadham, Michail Andreopoulos, Dominik Glodzik, Ruolin Liu, Lovelace J Luquette, Se-Young Jo, Azeet Narayan, Muchun Niu, Lisa Anderson, Joseph A Brew, Hsu Chao, Carrie Cibulskis, Guanlan Dong, Uday S Evani, William C Feng, Marta Grońska-Pęski, Adrienne Helland, Nazia Hilal, Nisrine T Jabara, Hu Jin, Ning Li, Monica D Manam, Shayna L Mallett, Alexi Runnels, Constantijn Scharlee, Carter Smith, Smaht Duplex Sequencing Focus Group, Diane Shao, Christopher A Walsh, Viktor A Adalsteinsson, Eunjung Alice Lee, Peter J Park, Kristin G Ardlie, Soren Germer, Richard A Gibbs, Sangita Choudhury, Harsha V Doddapaneni, Gilad D Evrony, Chenghang Zong, Tim H H Coorens
Faculty, Staff and Students Publications
Detecting somatic mutations in normal tissues is challenging due to sequencing errors and the low allele fractions of post-zygotic variants. Duplex sequencing greatly reduces errors and can detect mutations at any allele fraction, but systematic, cross-platform comparisons are lacking. We present a comprehensive benchmarking of six duplex sequencing technologies used by the SMaHT Network: CODEC, CompDuplex-seq, HiDEF-seq, NanoSeq, ppmSeq, and VISTA-seq. We evaluated their performance using cord blood DNA, a tumor-normal cell line mixture, and homogenates from six human tissues. Each method shows distinct profiles in genomic footprint, sensitivity, and cost. Despite differences in library construction and sequencing platforms, estimates …