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Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba

Electronic Theses and Dissertations

As sessile organisms, plants manage stress through complex signaling networks involving phytohormones such as phytosulfokine (PSK). PSK, a disulfated pentapeptide, regulates plant growth, development, and stress responses by interacting with specific PSK receptors (PSKRs). In this study, we explored the trafficking dynamics of PSK, its post-application fate, and the synthesis of an analog. We administered both native PSK and a fluorescent version tagged with TAMRA (5(6)-carboxytetramethylrhodamine) to various Arabidopsis thaliana genotypes, including wild type, a PSKR-deficient mutant, and a strain overexpressing PSKR1 tagged with green fluorescent protein (GFP) over the wild-type background. Fiber-optic fluorescence microscopy revealed that receptor presence influences …

Oh, My Quad: A Clinical Commentary And Evidence-Based Framework For The Rehabilitation Of Quadriceps Size And Strength After Anterior Cruciate Ligament Reconstruction, Braidy Solie, Mitchell Carlson, Christopher Doney, Michael Kiely, Robert Laprade

Physical Therapy Faculty Research and Publications

Quadriceps weakness after anterior cruciate ligament reconstruction (ACLR) is a well-known phenomenon, with more persistent quadriceps weakness observed after ACLR with a bone-patellar tendon-bone or quadriceps tendon autograft than with a hamstring tendon autograft. Longstanding quadriceps weakness after ACLR has been associated with suboptimal postoperative outcomes and the progression of radiographic knee osteoarthritis, making the recovery of quadriceps size and strength a key component of ACLR rehabilitation. However, few articles have been written for the specific purpose of optimizing quadriceps size and strength after ACLR. Therefore, the purpose of this review article is to integrate the existing quadriceps muscle basic …

The Disordered Negatively Charged C-Terminus Of The Large Hect E3 Ubiquitin Ligase Herc2 Provides Structural And Thermal Stability To The Hect C-Lobe, Kelly L. Waters, Kayla J. Rich, Noah D. Schwaegerle, Tianyi Yang, Shuanghong Huo, Donald E. Spratt

Chemistry

Homologous to the C-terminus of E6AP (HECT) and RCC1-like domain (RLD)-containing protein 2 (HERC2) is a large, 528 kDa E3 ubiquitin ligase that is associated with cancer, oculocutaneous albanism type 2, Prader-Willi syndrome, and other neurological diseases. HERC2 has been found to contribute to double-stranded DNA break repairs, tumor suppression, maintaining centrosome architecture, and ubiquitylation. The C-terminal portion of the HECT domain (C-lobe) of HERC2 is responsible for transferring ubiquitin to a substrate but the precise function of the other eight domains in HERC2 are unknown. Interestingly, HERC2 contains a unique and negatively charged C-terminal …

Rate Coefficients For Rotational State-To-State Transitions In H2O + H2 Collisions As Predicted By Mixed Quantum–Classical Theory, Carolin Joy, Dulat Bostan, Bikramaditya Mandal, Dmitri Babikov

Chemistry Faculty Research and Publications

Aims. A new dataset of collisional rate coefficients for transitions between the rotational states of H₂O collided with H₂ background gas is developed. The goal is to expand over the other existing datasets in terms of the rotational states of water (200 states are included here) and the rotational states of hydrogen (10 states). All four symmetries of ortho- and para-water combined with ortho- and para-hydrogen are considered.

Methods. The mixed quantum–classical theory of inelastic scattering implemented in the code MQCT was employed. A detailed comparison with previous datasets was conducted to ensure that this …

Constraining Decadal-Scale Erosion And Delivery Of Post-Wildfire Debris Flow Deposits, Casey Langstroth

All Graduate Theses and Dissertations, Fall 2023 to Present

Debris flows generated from wildfire pose significant risk to increased sedimentation to instream river networks, degradation of water quality, and accumulated sediment behind downstream reservoirs. Despite an abundance of research investigating the probability of debris flow generation, the constraints on deposit initial volume, and most recently the initial grain size distribution, there remains a significant knowledge gap in understanding the temporal scale at which debris flows supply sediment to river networks after deposition. To provide reliable estimates of sediment delivery from debris flow deposits over time, two important metrics must be constrained: 1) how does sediment delivery to river networks …

Clusteredlog: Optimizing Log Structures For Efficient Data Recovery And Integrity Management In Database Systems, Mariha Siddika Ahmad, Brajendra Panda

Electrical Engineering and Computer Science Faculty Publications and Presentations

In modern database systems, efficient log management is crucial for ensuring data integrity and facilitating swift recovery from potential data corruption or system failures. Traditional log structures, which store operations sequentially as they occur, often lead to significant delays in accessing and recovering specific data objects due to their scattered nature across the log. ClusteredLog addresses the limitations of traditional logging methods by implementing a novel logical organization of log entries. Instead of simply storing operations sequentially, it groups related operations for each data item into clusters. As a result, ClusteredLog enables faster identification and recovery of damaged data items …

“How Can I Teach When I Was Not Taught How?”: Teacher Education For Mother Tongue Instruction For Stem In Southwest Nigeria, Motunrayo Adeosun

Theses and Dissertations

In this qualitative study, the experiences, training, and challenges of teachers in primary schools in a community in southwest Nigeria were explored. The purpose of this study was to identify the experiences of teachers about their training for mother tongue instruction and how they teach pupils with the mother tongue or local language of the community, the challenges faced by teachers in teaching with the mother tongue, and the strategies used by teachers to overcome the challenges. This study found that teachers received no training and there is no curriculum for the training of teachers to teach in the mother …

Understanding Mental Health Awareness And Knowledge Of Flight Instructors, Eric Michael Buteau

Theses and Dissertations

Mental health has become an increasing concern in the aviation industry, with pilots often withholding health information for fear of losing their medical certification. Certified Flight Instructors (CFIs), who interact closely with students, are in a unique position to recognize and address mental health crises but lack sufficient training in this area. This study examines CFIs' awareness, knowledge, comfort, and willingness to engage with mental health issues in aviation. A survey was distributed to CFIs at a large aviation university. Survey results revealed no significant correlation between years of experience and knowledge or comfort with mental health topics. However, a …

Parp1 Modulates Gata3-Mediated Gene Regulation In Breast Cancer, Mikhala Cooper

Theses and Dissertations

In eukaryotic cells, chromatin acts as a physical barrier for many transcription factors, preventing them from efficiently binding to their recognition sequences within the nucleosome. Pioneer factors are a class of transcription factors that bind to nucleosomes and activate silent chromatin. Pioneer factors are frequently involved in various immune cell pathways, tissue development, and homeostasis, thereby underscoring their important roles in development and disease. Previous research found that pioneer factors have at least three fundamental properties: 1) they can interact with their cognate recognition sequences before transcription activation, 2) they can increase local chromatin accessibility, and 3) they have integral …

Advisor Impacts On Student Learning In Undergraduate Engineering Sae Competition Projects, Sam Ertl

Theses and Dissertations

This study examines the effects of advisor characteristics on student learning in large scale undergraduate engineering project competitions. A survey was conducted to measure statistical significance between student ratings of their advisors and student ratings of their learning in key skill areas. Additional items measured include demographics and project characteristics which allowed for additional analyses. The purpose of this study was to identify areas of improvement for project advisors to advance the learning potential for student participants. The survey was distributed in an online format to students participating in five SAE University Program projects. Collected data was analyzed using Kendall’s …

The Positivity Principle: Investigating A School Administrator Training Series Designed To Promote Systemic Positivity, Catherine Gillach

Theses and Dissertations

Education has historically been recognized as a stressful profession, yet the Covid-19 pandemic (2020) paired with intensified socio-political influences have exacerbated educator stress, anxiety, and negativity, prematurely pushing more career educators to leave the profession. Teacher and principal pre-service preparation programs largely neglect to train educators in strategies for personally managing negative stressors, and negativity mitigation strategies are often overlooked as a critical facet of school climate and culture. Therefore, the purpose of this dissertation in practice was to examine the degree to which a school administrator training series could stimulate personal and systemic positivity within participants’ schools in a …

An Investigation Into The Corrosivity Of Hydrocarbon Well Produced Water, Lucas James Jerylo

Theses and Dissertations

Produced water has been the largest byproduct of oil and gas production since the inception of the industry. Historically, very little concern was exhibited over what was done with this water. In recent decades, environmental concern has increased oversight on what happens to this produced water. Produced water is injected into deep formations, but this volume is limited due to concerns over seismic activity spawned from this practice. Produced water can be recycled in agricultural and municipal industries but it must first undergo a time-consuming and expensive cleaning process. This has turned oil and gas operators to utilizing this water …

Rational Design Of Engineered Soft Materials For Mineral Scale Control: Synthesis, Characterization, And Performance Evaluation, Nadhem Ismail

Theses and Dissertations

Inorganic crystallization, referred to as mineral scaling, have been posing tremendous operational challenges to water-based industries over the years. Multiple recent initiatives have focused on developing sustainable and highly effective antiscalants/scale inhibitors; however, most of the investigated antiscalants have demonstrated low scaling ion tolerance and unknown scale inhibitor-mineral crystal interaction. Engineered soft materials, particularly Quantum Dots (QDs) and biopolymer-based formulations, are among the most desirable solutions since they both solve critical environmental issues and offer insights into the inhibition mechanism. This dissertation reports on the development and performance evaluation of three distinct soft materials-based formulations; Carboxyl-Silicon Quantum Dots (CSiQDs), Carboxymethylcellulose …

Numerical Simulation And Assessment Of Underground Blue Hydrogen Storage In Depleted Gas Reservoir, Abraham Kazala

Theses and Dissertations

Nowadays, the world needs more energy than ever before. This need emphasizes that the world also requires a sustainable system that can meet these growing demands while addressing the overall impact on the environment. For this reason, the international community, including all levels of environmental policymakers, have shown a great deal of concern about the tremendous negative effects of fossil fuels on the globe. The impact of carbon dioxide as a Greenhouse Gas (GHG) from burning fossil fuels on the ozone layer and what it entails in global warming and, therefore, climate change has motivated research on finding either alternatives …

The Impact Of Land Use And Land Cover Change On Vegetation, Ecosystem Dynamics, And Conservation In The Northern Great Plains, Seth R. Fore

Theses and Dissertations

Land use and land cover change (LULCC) is one of the primary contributors to global biodiversity and habitat loss. Identifying sustainable development strategies requires understanding the social drivers of land use and conservation decisions as well as their impacts on ecological processes. These socio-ecological interconnections were explored in Northern Great Plains region. Results showed that common responses to socio-economic drivers have resulted in widespread loss of grassland areas throughout the region and that shifts in the functional characteristics of plant communities caused by LULCC have significantly altered key ecosystem functions linked to primary production, carbon/nutrient turnover, and water cycling. Expanding …

Applied Indigenous Knowledge Translation: Naatookyookaasii, Lynn Marie Mad Plume

Theses and Dissertations

This dissertation portfolio comprises three projects aimed at advancing Indigenous health and integrating cultural perspectives into research and practice. Guided by principles of community engagement and ethical research practices, these projects align closely with my career goals in Indigenous health policy and research. The first project, "Measuring Wellness Through Indigenous Partnerships," is a scoping review that examines global Indigenous wellness measures and assesses community involvement in defining measures of wellness. This review informs future practice directions and supports my advocacy for culturally responsive methodology. The second project, "Reciprocal Facilitation: Protocols and Practices for Listening Sessions with Tribal Communities," introduces a …

Personality Across Realities: Correlations Between Personality Profiles And Preferences Within Roleplaying Games, Brittany Lee Mancha

Theses and Dissertations

Clinically maladaptive behaviors and personality types have been studied within gamers, both those who play video games and those who roleplay in tabletop games. However, the connection between personality types and the preferences of the players themselves in what and how they roleplay is just barely being studied. Personality traits, such as extraversion/introversion and motivation by pain or pleasure, may be both influencing the gameplay decisions and preferences of players, and vice versa. A total of 500 respondents completed the Millon Index of Personality Styles, Revised (MIPS-R), followed by a demographics survey and finally a survey assessing Dungeons and Dragons', …

Cyclical Musicians: Collegiate Music Students’ Experiences Of Instrumental Musicking Processes Across The Menstrual Cycle, Anne Marques Catarin

Theses and Dissertations

Instrumental music researchers tend to focus on non-sex-specific health topics, such as posture (e.g., Garnett et al., 2022; Rousseau et al., 2023), musculoskeletal health (e.g., Mizrahi, 2020; Pratt et al., 2020), or neurological movement disorders (e.g., Altenmüller & Jabusch, 2010; Frucht, 2009). Despite the considerable extent of topics covered in musician health, the role of menstrual health in instrumentalists is currently underdeveloped. Using a case study design (Stake, 1995; Yin, 2014), I explored the unspoken experiences of collegiate instrumental music majors who menstruate, in an investigation to better understand the role of menstrual cycles in their musicking. For four months, …

Developing Inclusive Practices Through Peer Coaching In College Science Teaching, Briana Miller

Theses and Dissertations

Historically underrepresented students pursuing STEM majors encounter systemic barriers. Students are more likely to experience success when faculty implement inclusive practices. Faculty often lack the pedagogical training necessary to adopt inclusive practices, further perpetuating inequities. To eliminate barriers and offer equitable opportunities for success in STEM majors, this case study explores the implementation of peer coaching and Culturally Relevant Science Teaching (CRST) in college level science teaching, focusing on the experiences of three participating faculty. Through a year-long professional development (PD) cycle, three science faculty members from a small liberal arts college engaged in reflective practices aimed at improving their …

The Reservoir Geomechanics And Production Effects On Permafrost Stability During Polymer Eor, Shane S. Namie

Theses and Dissertations

The North Slope of Alaska (ANS) contains an estimated 40 billion barrels of heavy oil in relatively shallow formations (984 to 1,969 ft / 304 to 600 m) within the Prudhoe Bay Unit (PBU), Milne Point Unit (MPU) and Kuparuk River Unit (KRU). Extracting this resource presents unique challenges, especially when hydrocarbon-bearing zones are near the permafrost. Research on tertiary recovery processes in these shallow zones is limited, with prior studies indicating minor permafrost thawing and subsidence over 30 to 40 years but lacking a numerical simulation model supported by laboratory data.This study investigates the feasibility of an enhanced oil …

Petrophysical And Geomechanical Alterations During Supercritical Co2 Submerge: Laboratory Testing On Samples From The Middle Bakken, Ruichong Ni

Theses and Dissertations

The promising strategy of CO2 capture, utilization and storage (CCUS) aims to reduce CO2 emissions and tackle the pressing problem of global warming. Previous studies have demonstrated the importance of changes in petrophysical and geomechanical characteristics in assessing target formations' capacity to sequester CO2. Permeability is one of these characteristics that is essential to the movement of CO2 inside rock formations and has a direct effect on how well CO2 is sequestered. Existing methods calculate permeability by using effective stress, but they have made unpractical assumptions that the effective stress coefficient could be stable or gradually increase after CO2 exposure. …

Structural Degradation Causing Silicone Sealant Failure: A Comprehensive Analysis Using Evolved Gas Analysis And Thermal Desorption-Pyrolysis Gas Chromatography, Eugene Agbor Oga

Theses and Dissertations

Silicone sealants are essential components in various applications, but their long-term performance can be compromised by aging and weathering. This study investigated the thermal degradation behavior of poly dimethyl silicone sealants to gain insights into the underlying mechanisms of failure. A combined approach using Evolved Gas Analysis (EGA) and Thermal Desorption-Pyrolysis Gas Chromatography-Mass Spectrometry (TD-Pyr-GC-MS) was employed to analyze the degradation products and identify the factors influencing the degradation process.Aged and weathered sealants exhibited distinct thermal degradation profiles compared to fresh samples. The evolution of siloxane degradation products occurred at lower temperatures in aged sealants, indicating accelerated degradation. TD-Pyr-GC-MS analysis …

Risk Factor Patterns For Elder Abuse Among United States Indigenous Peoples: Native Elder Maltreatment Survey Secondary Data Analysis, Maylynn Riding In

Theses and Dissertations

Elder abuse is a significant public health issue, with findings indicating that 9% to 10% of older adults in the United States experience abuse. This figure is likely mirrored among Indigenous Peoples residing within tribal boundaries, although specific data is scarce. This study aims to fill the gap in understanding the prevalence and risk factors associated with elder abuse among United States Indigenous Peoples (USIP) through a secondary data analysis of the Native Elder Maltreatment Survey (NEMS). The NEMS employs two key instruments: the Hwalek-Sengstock Elder Abuse Screening Test (HS EAST), which is validated for community use, and the Native …

Elementary Teacher Instructional Self-Efficacy In Leader In Me Lighthouse Schools, Lara Storm

Theses and Dissertations

This study is a secondary data comparative analysis using the Leader in Me Measurable Results Assessment (MRA) to examine whether teachers at Leader in Me Lighthouse certified schools have a greater degree of instructional self-efficacy than teachers at non-Lighthouse certified Leader in Me schools. Literature shows that students in classes with teachers that have a higher degree of instructional self-efficacy experience higher student achievement and a more supportive classroom climate. In addition, those teachers with higher efficacy experience lower levels of stress and burnout and are more engaged in professional development. The guiding notion of this investigation is that implementing …

Handheld Multimodal Point Spectroscopy, Rgb Imaging, And Fusion Artificial Intelligence To Determine The Species And Freshness Of Fish Fillets, Mitchell Sueker

Theses and Dissertations

When fish species are mislabeled and the fish’s freshness is inaccurately represented, health, environmental, and economic problems can arise for both consumers and the fish industry. Fish are some of the easier foods to mislabel, as the processing of whole fish to fillets makes the visual identification of the species and freshness assessment more challenging. Due to the shortcomings of the current industry standards, confirmed by our interviews with over 20 professionals in all fish supply chain nodes, developing a system that can provide accurate and rapid on-site fraud detection is of necessity. In an attempt to meet these needs, …

Nanoparticles: Applications For Energy Innovation, Wen Sun

Theses and Dissertations

The work described in this dissertation will have three primary goals that are all essential for improving the energy demand using nanotechnology. The first goal is to comprehensively understand the application of SiO2-based nanoparticles (SiO2-based-NPs) in enhanced oil recovery (EOR) and the challenges associated with their use. The second goal is to investigate the application of ruthenium-based nanoparticles using reduced graphene oxide as the matrix (Runano-based/rGO) for the electrochemical synthesis of ammonia under ambient conditions. The third goal is to develop binder-free 3D nanomaterials and assess their electrochemical properties for supercapacitor applications. To fulfill this goal, two different nanomaterials have …

Development Of Fluorescent-Based Nanoparticles For Bioimaging, Gene Regulation Expression, And Photo-Induced Synergistic Cancer Therapy, Di Sun

Theses and Dissertations

Fluorescent-based nanoparticles have been applied to a broad range of biological applications, as discussed in this dissertation, including bioimaging, gene regulation expression, and photo-induced synergistic cancer therapy. Prior to applications, this dissertation first focused on the development and characterization of several fluorescent-based nanoparticles using different methods. These include 1) Streamlined synthesis of dual-emissive fluorescent-based silicon quantum dots (SiQDs). The SiQDs were generated as a promising, non-toxic labeling tool for bioimaging of a variety of cell types. both in visible and near-infrared (NIR) ranges. 2) Polymer-based nanoparticles - Polymer dots (Pdots) with a strong NIR fluorescence. The Pdots were functionalized as …

Investigating The Impact Of Urban Areas On Quasi-Linear Convective Systems, Claiborne Wooton

Theses and Dissertations

Urban areas absorb and radiate energy at a higher rate compared to surrounding rural areas due to several factors, including an urban area’s tendency to have sparse vegetation and tree canopies along with high incidences of pavement. Urban areas have been shown to impact both the environment in which thunderstorms form (by altering thermodynamic, microphysical, and wind flow patterns) and the thunderstorms themselves; however, most of this previous work focused on isolated thunderstorms amid weak environmental vertical wind shear. This thesis analyzes how the structure of observed quasi-linear convective systems (QLCSs)—i.e., lines of thunderstorms—are altered as they pass over urban …

The Fcc 5-Year Deorbit Rule’S Impact On The Space Industry Including Non-Traditional Scientific And Environmental Stakeholders, Kristin Shahady

Theses and Dissertations

In pursuit of a safer and more sustainable space environment, the Federal Communications Commission (FCC) introduced the 5-year deorbit rule, a regulatory mandate aimed at mitigating space debris by requiring satellite operators to remove their spacecraft from LEO within 5 years after their mission is complete. While the intent behind this rule is admirable in the desire to remediate and limit the generation of orbital debris, it raises questions whether it was crafted with consideration of all stakeholders, whether it will truly mitigate the creation of orbital debris, and whether it exhibits characteristics of arbitrariness and capriciousness. Agencies’ actions be …

Investigation Of Gas Injection Eor In Conventional And Unconventional Reservoirs Based On Laboratory Experiments And Field Pilot Tests, Jin Zhao

Theses and Dissertations

Currently, fossil fuels (including oil, natural gas, and coal) are still the dominant energy sources in the United States. However, their extraction and utilization generate a significant volume of carbon dioxide (CO2) and associated gases, such as methane (CH4), ethane (C2H6), propane (C3H8), etc. Injecting these gases back into deep oil reservoirs offers a practical means to enhance oil recovery while reducing air pollution. The target formations are usually characterized by good porosity and permeability to ensure sufficient injectivity and storage capacity. However, recent unconventional reservoir development indicated that tight formations could also be potential targets for enhanced oil recovery …