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Articles 121801 - 121830 of 1826496
Full-Text Articles in Entire DC Network
Harnessing Collective Structure Knowledge In Data Augmentation For Graph Neural Networks, Rongrong Ma, Guansong Pang, Ling Chen
Harnessing Collective Structure Knowledge In Data Augmentation For Graph Neural Networks, Rongrong Ma, Guansong Pang, Ling Chen
Research Collection School Of Computing and Information Systems
Graph neural networks (GNNs) have achieved state-of-the-art performance in graph representation learning. Message passing neural networks, which learn representations through recursively aggregating information from each node and its neighbors, are among the most commonly-used GNNs. However, a wealth of structural information of individual nodes and full graphs is often ignored in such process, which restricts the expressive power of GNNs. Various graph data augmentation methods that enable the message passing with richer structure knowledge have been introduced as one main way to tackle this issue, but they are often focused on individual structure features and difficult to scale up with …
Immediate Effect Of Local Vibration On Motor Unit Firing Behavior And Muscle Strength In Healthy Young Adult Males, Yuichi Nishikawa, Aleš Holobar, Kohei Watanabe, Toshihiko Komatsuzaki, Takanori Chihara, Jiro Sakamoto, Takashi Kawagoe, Hidetaka Nagayasu, Kyoko Mori, Kenji Kawano, Noriaki Maeda, Shinobu Tanaka, Allison S. Hyngstrom
Immediate Effect Of Local Vibration On Motor Unit Firing Behavior And Muscle Strength In Healthy Young Adult Males, Yuichi Nishikawa, Aleš Holobar, Kohei Watanabe, Toshihiko Komatsuzaki, Takanori Chihara, Jiro Sakamoto, Takashi Kawagoe, Hidetaka Nagayasu, Kyoko Mori, Kenji Kawano, Noriaki Maeda, Shinobu Tanaka, Allison S. Hyngstrom
Physical Therapy Faculty Research and Publications
Purpose
The aim of this study was to examine the effect of vibration on motor unit (MU) firing behavior and physical performance of antagonist muscles in healthy young adult males.
Methods
Fourteen males (age = 24.3 ± 3.6 years) were included in this study. There were two conditions, one in which participants received 80 Hz vibration in the distal tendon of the hamstring for 30 s and the control condition (no vibration). High-density surface electromyography (HD-SEMG) signals and maximal voluntary contraction (MVC) of knee extensor muscles were evaluated before and after the respective conditions and recorded from the vastus lateralis …
Relative Muscular Effort In The Older Adults During The Sit-To-Stand Task: Monitoring Neuromuscular Reserve And Movement Limitations, Nayun Ahn, Michael H. Haischer, Cara L. Lewis, Kristof Kipp
Relative Muscular Effort In The Older Adults During The Sit-To-Stand Task: Monitoring Neuromuscular Reserve And Movement Limitations, Nayun Ahn, Michael H. Haischer, Cara L. Lewis, Kristof Kipp
Exercise Science Faculty Research and Publications
Background
Due to lower maximum strength, older adults purportedly need to exert greater muscular effort to stand up from a chair. This study investigated joint-specific muscular effort during a sit-to-stand by calculating relative-muscular-effort and investigating differences between healthy older and young adults.
Methods
Fifteen older (age: 69.9 ± 3.5 years) and 15 young (age: 22.2 ± 2.6 years) adults performed five self-paced sit-to-stand trials. Kinematic and kinetic data were collected and used to calculate inverse dynamics net joint moment. Peak isometric knee and hip extensor torques were measured and used as inputs to regression models that predicted angle- and velocity-dependent …
Cognitive Apprenticeship Strategies For The Media Literacy Classroom, Nate Floyd, Jaclyn Spraetz
Cognitive Apprenticeship Strategies For The Media Literacy Classroom, Nate Floyd, Jaclyn Spraetz
Communications in Information Literacy
Inspired by the apprenticeship model of teaching and learning, two instructors report on their efforts to place current events at the center of a semester-long media and information literacy course. They discuss strategies to harness curiosity about contemporary topics (e.g., misinformation, climate change, algorithms, right-to-repair, blockchain, artificial intelligence, etc.), and create engagement-driven course content. The paper provides concrete examples of activities and assignments used to situate learning in real-world contexts and build students' confidence in navigating information independently. This case study offers insights for other educators seeking to apply cognitive apprenticeship principles to media and information literacy instruction.
Building On Strengths: A Collaborative, Asset-Focused Approach To Teaching Critical Information Literacy Skills, Rebecca B. Penrose, Kristi J. Chavez
Building On Strengths: A Collaborative, Asset-Focused Approach To Teaching Critical Information Literacy Skills, Rebecca B. Penrose, Kristi J. Chavez
Communications in Information Literacy
This article showcases an instructor–librarian collaborative model for teaching critical information literacy (CIL) skills in a higher education course by incorporating interactive workshops into a sequence of required course assignments. Using an asset-focused design, this assignment sequence allows students to first demonstrate their existing research strategies and then evaluate those strategies during workshop activities, applying the CIL principles they are learning. In this way, the approach recognizes students’ existing strengths and builds upon them, while emphasizing self-reflection opportunities and an intentional research focus on diversity, equity, inclusion, and representation. Results from our one-year study are also highlighted. The suggested instructional …
Centering Relevance In Information Literacy Teaching And Learning, Anne Jumonville Graf
Centering Relevance In Information Literacy Teaching And Learning, Anne Jumonville Graf
Communications in Information Literacy
The process of determining whether a source of information is relevant is multidimensional, dynamic, and subjective. This essay puts information science scholarship on relevance, including the process and nature of making relevance judgments, in conversation with models of teaching and learning information literacy. Teaching librarians are encouraged to recognize students’ relevance judgments as sites of reflection and instruction. This essay suggests a variety of ways librarians might do this, from re-thinking source evaluation methods to emphasizing the opportunities available at the source selection stage. The process of determining relevance is a practical site of reflective possibility and deserves greater attention …
Environmental Light Controls The Daily Organization Of Breathing By Activating Brn3b-Expressing Intrinsically Photosensitive Retinal Ganglion Cells In Mice, Aaron A. Jones, Allison R. Spears, Deanna M. Arble
Environmental Light Controls The Daily Organization Of Breathing By Activating Brn3b-Expressing Intrinsically Photosensitive Retinal Ganglion Cells In Mice, Aaron A. Jones, Allison R. Spears, Deanna M. Arble
Biological Sciences Faculty Research and Publications
Rhythmic, daily fluctuations in minute ventilation are controlled by the endogenous circadian clock located in the suprachiasmatic nucleus (SCN). While light serves as a potent synchronizer for the SCN, it also influences physiology and behavior by activating Brn3b-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs). It is currently unclear the extent to which the external light environment shapes daily ventilatory patterns independent of the SCN. To determine the relative influence of environmental light versus circadian timing on the organization of daily rhythms in minute ventilation, we used whole-body plethysmography to measure the breathing of mice housed on a non-entraining T28 cycle …
Does Self-Care Moderate The Association Between Adverse Childhood Experiences, Trauma Symptoms, And Parental Reflective Functioning?, Vinaya Thomas
Does Self-Care Moderate The Association Between Adverse Childhood Experiences, Trauma Symptoms, And Parental Reflective Functioning?, Vinaya Thomas
Electronic Theses and Dissertations
Unresolved adverse and traumatic experiences in parents can amplify the risk of perpetuating intergenerational transmission of trauma. Parents’ positive and adaptive practices such as self-care might act as a protective factor against this ripple effect. This study investigated whether self-care moderated the relationship between parental ACEs, post-traumatic stress symptoms, and parental reflective functioning in mothers who are experiencing high psychosocial stress (n = 59) residing in rural Appalachia. Within our sample, there was no statistically significant linear relationship between our predictors, ACE score and PTSD symptoms, and our outcome of interest, maternal pre-mentalizing. We did not find empirical support for …
Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams
Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams
Electronic Theses and Dissertations
Carnivorous plants face significant conservation challenges due to habitat destruction, climate change, and illegal harvesting. This study explores how elevation and population size influence key-life history traits of D. rotundifolia, such as flowering phenology, prey capture efficiency, seed production, and germination rates. Higher elevations and larger populations are associated with extended flowering durations and increased prey capture. However, seed production and germination rates show complex patterns that are likely influenced by genetic diversity, resource availability, or environmental conditions. These insights inform targeted conservation strategies, emphasizing habitat preservation, enhancing prey availability, and maintaining genetic diversity. Public education and community engagement …
Graduation Rates Of Students With Disabilities At A Participating Southern University, Kelly Mitchell
Graduation Rates Of Students With Disabilities At A Participating Southern University, Kelly Mitchell
Electronic Theses and Dissertations
The purpose of this non-experimental, correlational quantitative study was to compare the graduation rates of first-time, full-time students with disabilities to those of first-time, full-time students in the general population at the participating university. The sample used for analysis was full-time, first-time students entering between 2015 and 2020. Using archival data from the Disability Services database and the University's Banner system, four- and six-year graduation rates were compared. The original data set consisted of 22,070 students in the starting focus cohorts. After removing entering transfer students and students who enrolled in less than 15 credits for their entering semester, 8,337 …
Mapping Amis Mill In Rogersville, Tennessee In The 1780s Using Geophysical Methods And Remote Sensing, Amy Collins
Mapping Amis Mill In Rogersville, Tennessee In The 1780s Using Geophysical Methods And Remote Sensing, Amy Collins
Electronic Theses and Dissertations
Geophysics and remote sensing were used at the Amis Mill and Homesite to determine the location of structures during the 1780s. Areas of interest were surveyed using shallow geophysical and remote sensing techniques including the orchard, horse pasture, original kitchen on the east side of the house, the west side of the house, plowed field, store site, and cemetery. After analyzing the results of the geophysical surveys and remote sensing, 22 sites were chosen to test inside and outside potential features. A large rectangular feature, possibly a tavern with a cellar, was discovered. Four other features were identified, three of …
Detecting Anomalies In Dynamic Attributed Graphs: An Unsupervised Learning Approach, Austin Hamilton
Detecting Anomalies In Dynamic Attributed Graphs: An Unsupervised Learning Approach, Austin Hamilton
Electronic Theses and Dissertations
Dynamic attributed graphs, which evolve over time and hold node-specific attributes, are essential in fields like social network analysis, where anomalous node detection is a growing area. Vehicular social networks (VSNs), a subset of these graphs, are ad hoc networks in which vehicles exchange data with one another and with infrastructure. In this dynamic context, identifying anomalous nodes is challenging but crucial for maintaining trust within the network. This work presents an unsupervised deep learning approach for anomalous node detection in VSNs. This model achieved an accuracy of 71% while detecting synthetic anomalies in a simulated network based on real-world …
Perceived Stigma's Influence On The Association Between Suicide Risk Severity And Help-Seeking For Suicidal Crises, Donna Zastrow
Perceived Stigma's Influence On The Association Between Suicide Risk Severity And Help-Seeking For Suicidal Crises, Donna Zastrow
Electronic Theses and Dissertations
Introduction: Unfortunately, research examining help-seeking for suicide is limited. This study examined age, race, gender, suicide risk severity, and perceived stigma as predictors of formal and informal help-seeking behaviors individuals engage in during suicidal crises, and explored perceived stigma as a moderator of the relationship between risk severity and help-seeking. Method: Data from 80 college students who had experienced a past suicidal crisis were analyzed using two moderation analyses. Results: Rates of formal (67.5%) and informal (86.2%) help-seeking were high in the study sample. Higher suicide risk severity was positively associated with formal help-seeking (b = …
Community College Student Success During Covid-19 Pandemic Based On Class Modality, Rebecca Aslinger
Community College Student Success During Covid-19 Pandemic Based On Class Modality, Rebecca Aslinger
Electronic Theses and Dissertations
The purpose of this non-experimental, quantitative, comparative study was to determine whether there were significant differences in Introduction to Education grades as measured by GPA among community college students between 2018-23 with the change of class modality due to the Covid-19 pandemic.
This study examined differences in Introduction to Education grades as measured by GPA at three community colleges from 2018-23. Archival data were used for this study. The population for this study consisted of all students enrolled in the Introduction to Education course at three institutions. Both in-person and online classes were examined during the time before, during, and …
Heat Acclimated Algae Improves Heat Tolerance And Longevity In Daphnia, Ashit Dutta
Heat Acclimated Algae Improves Heat Tolerance And Longevity In Daphnia, Ashit Dutta
Electronic Theses and Dissertations
Xenohormesis suggests that consuming stressed food provides physiological adaptation to the consumer for similar stresses. To evaluate this, Daphnia magna was provided with heat-stressed Nannochloropsis limnetica grown at 25℃ whereas the control group was provided Nannochloropsis limnetica grown at 12℃. In the heat tolerance experiment, Daphnia kept at 28℃ and 20℃ while consuming 25℃ algae had significantly higher heat tolerance compared to the control. Additionally, in stressed conditions, Daphnia consuming 25℃-grown food had significantly higher lifespan compared to the control. Next, Daphnia consuming 25℃-grown algae had significantly higher levels of antioxidants which could explain the lifespan extension. Finally, in fatty …
College Student’S Learning Experiences During Covid-19 Among Traditional And Post-Traditional Students Enrolled In Institutions Affiliated With The Appalachian College Association, Jo Anne Smith
Electronic Theses and Dissertations
The purpose of this study was to determine the differences in the learning experiences, feelings and emotions, level of study support, and online learning during the COVID-19 pandemic between traditional and post-traditional college students enrolled in an institution affiliated with the Appalachian College Association.
A two-group comparison research design using a web-based survey was used for the study. Using the College Student’s Learning Experiences during COVID-19 among Traditional and Post-Traditional Students Enrolled in Institutions Affiliated with the Appalachian College Association survey, the research questions were analyzed with a sample size of N = 58, traditional college student group and N=33, …
Educator Perceptions Of Career And Technical Education Courses And The Involvement In Career And Technical Student Organizations On Student Attendance, Robert Satterfield
Educator Perceptions Of Career And Technical Education Courses And The Involvement In Career And Technical Student Organizations On Student Attendance, Robert Satterfield
Electronic Theses and Dissertations
The purpose of this study was to investigate the perceptions of secondary teachers, administrators, and educational supervisors of the implications of Career and Technical Education courses and the involvement in Career and Technical Student Organizations on student attendance. Data for this study were collected quantitatively by utilizing Microsoft Forms to administer the survey to potential participants. The survey administered for this study contained 13 Likert scale questions and two open-ended questions. Once data were collected SPSS Version 25 was used to analyze the data from the survey. After data analysis, it was concluded that all 13 of the Likert scale …
Landslide Inventory And Unstable Slope Monitoring Along Highways In Eastern Tennessee, Robert Mcsweeney
Landslide Inventory And Unstable Slope Monitoring Along Highways In Eastern Tennessee, Robert Mcsweeney
Electronic Theses and Dissertations
This research introduces an unstable slope management program (USMP) for Tennessee based on federal slope management standards, along with improved methods for landslide monitoring with unmanned aerial systems (UAS) lidar and photogrammetry. In mountainous regions, monitoring slope hazards is a critical function of transportation management. A mobile field assessment form created with Survey123 was used to collect 22 unstable slope ratings in eastern Tennessee. Location points were appended with photographs, notes, and site information. Landslide scores ranged from 325 (Fair) to 1005 (Poor). UAS monitoring of a slow-moving soil landslide along I-40 near Rockwood, TN, produced high-resolution lidar and photogrammetry …
Investigating Phytosulfokine Trafficking: Insights Into The Role Of Phytohormones In Plant Signaling, Martin Tindi
Investigating Phytosulfokine Trafficking: Insights Into The Role Of Phytohormones In Plant Signaling, Martin Tindi
Electronic Theses and Dissertations
Phytosulfokine is a critical signaling peptide involved in plant growth, development, and stress responses. This thesis investigates the trafficking mechanisms of Phytosulfokine (PSK) in Arabidopsis thaliana, employing fluorescently labelled PSK for non-destructive imaging using a custom-built fiber-optic fluorescence microscope. The study aims to determine the mobility and fate of PSK in plant tissues. Experimental results reveal the short-range and long-range movement of PSK across leaves. Additionally, the project highlights the optimization of non-destructive imaging techniques and the synthesis of caged dexamethasone to facilitate optogenetic studies. These findings provide new insights into PSK trafficking mechanisms in plants, enhancing our understanding …
The Effectiveness Of Online Learning Technologies In Zimbabwean Primary And Secondary Schools During The Covid-19 Pandemic (2020-2022), Kudakwashe Magwenzi
The Effectiveness Of Online Learning Technologies In Zimbabwean Primary And Secondary Schools During The Covid-19 Pandemic (2020-2022), Kudakwashe Magwenzi
Electronic Theses, Projects, and Dissertations
ABSTRACT
Zimbabwe, a country with modest technological infrastructure and insufficient government support systems, was forced to switch to online instructions almost overnight as the COVID-19 pandemic raged on. This research investigates the effectiveness of learning technologies applied in Zimbabwean primary and secondary schools during the pandemic based on responses gathered from surveys and in-depth interviews with various stakeholders. This research was informed by the essences of constructivism and connectivism theories that say that optimal learning takes place through engagement, collaboration, and interaction and educational technologies potentially can foster effective learning by facilitating access to information, enabling peer interactions and utilization …
The Impact Of Benevolent Sexism On Evaluations Of Female Leaders, Daniella A. Lockhart
The Impact Of Benevolent Sexism On Evaluations Of Female Leaders, Daniella A. Lockhart
Electronic Theses, Projects, and Dissertations
This study investigated the impact of benevolent sexism beliefs on evaluations of female leadership candidates in hiring decisions, as well as the moderating effects of occupational gender composition and the gender of the comparison candidate. While seemingly positive, benevolent sexism subtly reinforces traditional gender roles by portraying women as warm but less competent, particularly in leadership roles where competence is crucial. The data in this study was collected from 146 participants via an online questionnaire using MTurk and snowball sampling. Aligning with previous research on gender bias, results indicated that benevolent sexism negatively impacts perceptions of competence and warmth, especially …
The Impact Of Mindfulness On Student Engagement: During And After The Covid-19 Global Pandemic, Michelle Mendiola
The Impact Of Mindfulness On Student Engagement: During And After The Covid-19 Global Pandemic, Michelle Mendiola
Electronic Theses, Projects, and Dissertations
This qualitative study explored the lived experiences of educational leaders who incorporated mindfulness practices (MFP) student engagement (SE) in their classrooms during and after the COVID-19 global pandemic, utilizing hermeneutic phenomenology and bracketing as research methodologies. The study aimed to understand how these leaders' use of mindfulness impacted student engagement in both online and in-person learning environments. Drawing on semi-structured qualitative interviews, the research examined how mindfulness practices were adapted in response to the unique challenges posed by remote education during the pandemic and how these adaptations have persisted or evolved in post-pandemic teaching. Key findings reveal that mindfulness not …
The Impact Of Wellness Programs On Teacher Stress And Coping, Jessica Brinkley
The Impact Of Wellness Programs On Teacher Stress And Coping, Jessica Brinkley
Electronic Theses, Projects, and Dissertations
This qualitative study explored the lived experiences of ten secondary-level educators and their perceptions of work-related stress and wellness programs as a coping mechanism. Participants were middle and high school teachers in one school district in the Pacific Northwest that has a wellness program. Through semi-structured interviews, it employs a transcendental phenomenological analysis to merge participants’ experiences with Lazarus and Folkman’s (1987) Transactional Model of Stress and Coping. The research underscores the need for systemic changes to address teacher stress and well-being before wellness programs can serve as an effective coping mechanism. Critical themes found are High Workload and Time …
Development Of An In Vitro Squamous Metaplasia Model Using Airway Basal Stem Cells, Arash Tahmasbi
Development Of An In Vitro Squamous Metaplasia Model Using Airway Basal Stem Cells, Arash Tahmasbi
Electronic Theses, Projects, and Dissertations
Squamous metaplasia (SM) of the airway epithelium is an abnormal condition that is caused by exposure to airborne toxicants, cigarette smoke, pollutants, and pathogens. Since SM of the airway was first documented in the 1970s, data on this epithelial disease remain limited. More research is needed to fully understand this epithelial disorder and to prevent its progression into severe illnesses such as chronic bronchitis (CB), COPD, and squamous cell carcinoma. We developed 3D models of SM using airway basal stem cells (ABSCs) isolated from lung obtained from postmortem human donors. The ABSCs were cultured at the air-liquid interface (ALI) for …
The Effects Of Covid-19 Lockdowns On Cybersecurity, Natalie Sanders
The Effects Of Covid-19 Lockdowns On Cybersecurity, Natalie Sanders
Electronic Theses, Projects, and Dissertations
The COVID-19 pandemic and subsequent lockdowns forced many Americans to quickly adapt to working from home, many of which had never done so in the past. In addition, organizations were forced to modify security policies and protocols to allow for remote access to sensitive information. The rapid change in security policies as well as the sudden growth of remote access during the pandemic presented a broader landscape for cyber criminals to attack. In this report, we review the number and type of cyberattacks reported from two (2) years before the pandemic through two (2) years after (1998 through 2023), to …
Personalities Of Victims: A Look Into What Makes The Human Mind More Susceptible To Social Engineering, Matthew Ulloa
Personalities Of Victims: A Look Into What Makes The Human Mind More Susceptible To Social Engineering, Matthew Ulloa
Electronic Theses, Projects, and Dissertations
Social engineering is a frequent attack used to steal information from victims. This attack has plagued businesses worldwide for many years and continues to get worse every year as it deals with the human aspect of cyber security, something often ignored in cyberspace. Humans have been deemed as the weakest link in cyber security. Previous researchers have taken it upon themselves to discover links between the human mind and social engineering. This graduate research paper was conducted on the foundation of these research papers, based on the Five Factor Model of Personalities (FFM) and susceptibility to social engineering. This research …
Assessing Khumbu Region Glaciers Mass Balance Change Using Geospatial Techniques From 2012 To 2024, Yashvi Jagatbhai Shah
Assessing Khumbu Region Glaciers Mass Balance Change Using Geospatial Techniques From 2012 To 2024, Yashvi Jagatbhai Shah
Electronic Theses, Projects, and Dissertations
The glaciers in the Hindu Kush Himalaya are critical for sustaining the water supply of South Asia's major rivers, upon which over 230 million people, both in the mountainous regions and lowland areas, depend. Climate change is driving rapid glaciers melt near the summit of Mount Everest, located at an elevation of approximately 7,906 meters (25,938 feet). This has resulted in glacier thinning occurring up to 80 times faster than the original ice formation. However, due to their disposition in a complex topographic setting and inaccessible terrain, continuous glacier observations pose significant challenges.
This study evaluated the mass balance of …
Señoritas On And Off The Silver Screen: How Chicana Women Organized For Equality And Challenged The Cultural Prison Of Sexualized Media Representations In The First Half Of The Twentieth-Century, Rossandra Paloma Martinez
Señoritas On And Off The Silver Screen: How Chicana Women Organized For Equality And Challenged The Cultural Prison Of Sexualized Media Representations In The First Half Of The Twentieth-Century, Rossandra Paloma Martinez
Electronic Theses, Projects, and Dissertations
This research is personal for me due to lived experience and day-to-day situations where I have felt the pressures of unattainable beauty standards and society’s fluid definition of beauty and femininity surrounding Chicana, Latinas, Mexican, and Mexican-American women. What inspired me to pursue these issues and topics was the work of Dr. Vicki Lynn Ruiz in her book From Out of the Shadows: Mexican Women in Twentieth-Century America. This research focuses on rival and contested images of Chicana, Mexican, Mexican-American and Latina labor advocates and activists from the early twentieth as opposed to sexualized and eroticized packaged caricatures that Hollywood …
Anaplasma Phagocytophilum’S Effect On Gopher Tortoises Of South East Florida, Elena Tonkin
Anaplasma Phagocytophilum’S Effect On Gopher Tortoises Of South East Florida, Elena Tonkin
Harriet L Wilkes Honors College Theses 2020 - Present
Recent studies have revealed Anaplasma phagocytophilum as a new pathogen that has started to affect gopher tortoise populations throughout Florida. This study investigated how the pathogen, A. phagocytophilum, has potentially begun to spread amongst the gopher tortoises in the Abacoa Greenway and the Florida Atlantic University Preserve (FAUP). To investigate these tortoises, blood samples were taken from multiple specimens from Range VI of the Greenway and FAUP. DNA isolated from these samples were then analyzed for the presence of A.phagocytophilum utilizing PCR and gel electrophoresis, followed by Sanger sequencing. This research demonstrated that A.phagocytophilum has not made its way …
Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba
Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba
Electronic Theses and Dissertations
As sessile organisms, plants manage stress through complex signaling networks involving phytohormones such as phytosulfokine (PSK). PSK, a disulfated pentapeptide, regulates plant growth, development, and stress responses by interacting with specific PSK receptors (PSKRs). In this study, we explored the trafficking dynamics of PSK, its post-application fate, and the synthesis of an analog. We administered both native PSK and a fluorescent version tagged with TAMRA (5(6)-carboxytetramethylrhodamine) to various Arabidopsis thaliana genotypes, including wild type, a PSKR-deficient mutant, and a strain overexpressing PSKR1 tagged with green fluorescent protein (GFP) over the wild-type background. Fiber-optic fluorescence microscopy revealed that receptor presence influences …