Digital Commons Network^™

A High-Efficiency Gene Silencing In Plants Using Two-Hit Asymmetrical Artificial Micrornas, Sachin Teotia, Xiaoran Wang, Na Zhou, Mengmeng Wang, Haiping Liu, Jun Qin, Dianwei Han, Chingwen Li, Christine E. Li, Shangjin Pan, Haifeng Tang, Wenjun Kang, Zhanhui Zhang, Xiaoqing Tang, Ting Peng, Guiliang Tang

Michigan Tech Publications, Part 1

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. They are produced through an enzyme-guided process called dicing and have an asymmetrical structure with two nucleotide overhangs at the 3′ ends. Artificial microRNAs (amiRNAs or amiRs) are designed to mimic the structure of miRNAs and can be used to silence specific genes of interest. Traditionally, amiRNAs are designed based on an endogenous miRNA precursor with certain mismatches at specific positions to increase their efficiency. In this study, the authors modified the highly expressed miR168a in Arabidopsis thaliana by replacing the single miR168 stem-loop/duplex with …

Tennessee State Museum Spring 2023 Newsletter & Calendar Of Events, Tennessee. State Museum.

Tennessee State Museum

No abstract provided.

Chapter 25- Mentoring Programs For Staff Of Educational Institutions: Unm Staff Council Mentorship Program, Amy Hawkins

Making Connections

In higher education, staff sometimes feel like the third wheel, the step-child, the forgotten ones sitting on the sidelines as students and faculty bask in the warm glow of academia. Administrators in university settings owe duties to (a) faculty and student needs; and (b) staff development, morale, needs, pay, and benefits. The University of New Mexico’s Staff Council was created so that volunteer university staff elected to serve as councilors can advocate for staff by offering recommendations to the university regarding staff development, morale, needs, pay, and benefits. Each can bring constituent concerns to the full Staff Council and its …

Chapter 29- Conclusion, David Law, Nora Domínguez

Making Connections

Too often, formal mentoring programs are started at universities without thinking through and addressing the details needed for the program to succeed. As stated at the beginning, the primary purpose of this handbook is to provide a “one-stop shop” resource that guides program coordinators to be intentional and effective in designing, implementing, evaluating, sustaining, and funding their academic mentoring program. In this concluding section, we describe how this book’s chapters and case studies connect to form a comprehensive guide for program coordinators and other stakeholders. Making the chapter’s interconnections explicit makes a needed contribution to the mentoring field, particularly as …

Political Theory And The Volunteer: Lessons From Kahn’S Ethnography Of ‘Our Unhappy Politics’, Benjamin Berger

All Papers

This article offers a reading of Paul Kahn’s Democracy in Our America that places this intimate “work of local political theory” in a central position in the landscape of his political thought. The article argues that the figure of the volunteer, as it appears in the volume, holds a space for love and meaning—and for political happiness—that secures for it a critical role in the system of beliefs and practices that sustain self-government in the United States. That framing draws the volunteer into relationship with Kahn’s thinking about the family, the veteran, and law. But it also means that the …

Disrupting Routine: The Expansion Of Precedent, Olena Yarmolyuk, Olena Yarmolyuk

Masters in Architecture Program: Theses

Iconic architecture has presented a preferential nature to the establishment of architectural work. Academically, only the architectural a-side is presented to students. It is used as a means to develop, measure, and identify good work. Meanwhile, the architectural B-side is deliberately hidden away and censored by the profession. It exploits the perverse - displaying all of architecture’s failures, glitches, and anomalies.1 However, the notion of the a and b sides also presents problematic consequences. Prescribing architecture as either a or b side is problematic - it creates a divide between iconic architecture and all the other works deemed ‘insignificant’. Even …

It's A Bitter Sweet Symphony: Licensing Complexities And Copyright Law In The Music Industry., Sadie Lorence

Honors College Theses

Historically, there has consistently been tension in the music industry between the ideas of ownership and accessibility. This past semester, I researched the history of copyright law and how it has evolved in regard to the licensing of musical works. I have conducted a content analysis of The U.S. Copyright Act in the last century to gain insight into its evolution and advancements. After I gathered an adequate understanding of intellectual property law within the realm of the music industry, I focused my case study research on one milestone example of copyright law’s relationship to issues of licensing and ownership …

Development And Validation Of An Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry Method To Investigate The Plasma Pharmacokinetics Of A KCa2.2/KCa2.3 Positive Allosteric Modulator In Mice, Mohammad Asikur Rahman, Devaraj Venkatapura Chandrashekar, Young-Woo Nam, Basir Syed, David Salehi, Hamidreza Montazeri Aliabadi, Miao Zhang, Reza Mehvar

Pharmacy Faculty Articles and Research

Rationale

There is currently no treatment for spinocerebellar ataxias (SCAs), which are a group of genetic disorders that often cause a lack of coordination, difficulty walking, slurred speech, tremors, and eventually death. Activation of K_Ca2.2/K_Ca2.3 channels reportedly exerts beneficial effects in SCAs. Here, we report the development and validation of an analytical method for quantitating a recently developed positive allosteric modulator of K_Ca2.2/K_Ca2.3 channels (compound 2q) in mouse plasma.

Methods

Mouse plasma samples (10 μL) containing various concentrations of 2q were subjected to protein precipitation in the presence of a structurally similar …

Spike-Timing-Dependent Plasticity Alters Sensory Network Connectivity, Adalee Lube

Arts & Sciences Graduate Student Theses and Dissertations

A fundamental question in neuroscience is: how does a sensory system optimize detection of behaviorally relevant stimuli, when those stimuli and the sensory environment are constantly changing? Spike-timing-dependent plasticity (STDP), in which synapse strength changes based on the relative timing of pre- and post-synaptic spiking, has been implicated in changes in neuronal connectivity thought to underlie learning and memory. Synaptic strength changes caused by STDP have been shown in optic tectum, visual cortex, hippocampus, and other brain regions in vitro across many organisms like fish, frogs, and mice. Although it is possible that STDP mechanisms underlie changes in sensory neuron …

A Mechanistic Study Of Err Α/Γ Agonists For Treatment Of Metabolic Dysfunction In Heart Failure, Mckenna Lael Losby

Arts & Sciences Graduate Student Theses and Dissertations

Heart failure is the leading cause of death in the United States and current therapeutic interventions fail to reverse the disease progression. In the failing heart, pathological cardiac remodeling leads to disease progression: a process characterized by hypertrophy, inflammation, fibrosis, and metabolic remodeling. By pharmacologically targeting the nuclear hormone receptors estrogen receptor-related receptor α and γ (ERRα and γ), it may be possible to reverse the array of metabolic pathways that are pathologically inhibited in the failing heart. Fatty acids are the primary fuel source of the adult heart that generate the ATP required for each contraction; however, in heart …

Experimental Investigation Of Non-Hermiticity In Quantum Systems, Maryam Abbasi

Arts & Sciences Graduate Student Theses and Dissertations

The investigation of quantum systems in experimental setups involves interaction with the environment, which introduces channels of dissipation. These systems can be described by a non-Hermitian Hamiltonian, capturing the effect of gain and loss. Realizing these systems in classical cases demonstrates interesting phenomena such as topological features in the optical and mechanical domain, energy transfer, and enhancement in sensing. The primary focus of this thesis is the realization of the non-Hermiticity in a quantum system. To accomplish this, we harness multiple energy levels of an anharmonic superconducting circuit with coupling to an engineered bath to investigate the static and dynamic …

Financial Literacy In College Today: Is It Needed?, Jasper Tatsuya Lem

Undergraduate Honors Theses

Californians are facing several financial crises, headlined by 4.5 million Californians falling below the poverty line, a 4.2% unemployment rate, and the fall of Silicon Valley Bank in early March 2023. In the face of these perilous circumstances, it is worth asking if California’s education system could be improved to accommodate more financial literacy classes. Currently, the Golden State does not mandate a financial literacy course in any level of education, kindergarten through senior year of college. Previous research indicates that financial literacy courses would be more effective if mandated in college courses rather than high school. However, there has …

The Role Of Narrative Medicine In Patient Healing And Meaningful Clinical Practice, Elizabeth Gray Duperier

Honors Theses

The patient-clinician relationship is one that relies heavily on trust and understanding—both of which are reached through effective communication. As medicine advances, it is increasingly important to prioritize the human interaction in the clinical encounter. Illness is more than just a set of diagnoses; it is the culmination of one’s way of being and interacting with the world while experiencing these diagnoses—it is a narrative. In order for physicians to provide quality care, trust and understanding are of the utmost importance, but oftentimes these are not at the forefront of medical practice due to logistical barriers that the US healthcare …

(Un)Constitutional Carry: How Drug And Gun Laws Conspire In Stand Your Ground States To Create A Disarmed, Submissive Class Based On Race, Jackson Whetsel

University of Memphis Law Review

No abstract provided.

“Speechless, Placeless Power”: Affect And Trauma In Moby-Dick And “Bartleby, The Scrivener”, Lauren Colandro

Seton Hall University Dissertations and Theses (ETDs)

Herman Melville’s Moby-Dick and “Bartleby, the Scrivener” contain affectively unsound figures such as Captain Ahab and Bartleby that seem to disrupt larger narrative functions, both developing these characteristics in response to prior trauma. However, narrators are not privy to the extent of their feelings because of their idealistic attachments to the disruptive figures. This thesis examines the commonalities of Melville’s disruptive characters in both stories using affect theory, as well as how their disruptions illuminate the effects of repressed trauma in an increasingly capital-driven society.

West Side Marquis Llc V. Moret

All Decisions

No abstract provided.

Dubai Emirate Land Price Prediction Using Analytics And Machine Learning, Shaikha Ali Mohsin Alattar Alhashmi

Theses

The Dubai Emirate is a burgeoning and vibrant region that has gained significant attention in the real estate industry due to its rapid development. With an increase in demand for land, the region has experienced a substantial surge in land prices. Therefore, precise land price prediction has become paramount for real estate investors, developers, and government officials. The purpose of this study was to employ analytics and machine learning techniques to accurately forecast land prices in the Dubai Emirate.

The study employed a dataset that included influencing factors such as, location, amenities, infrastructure, size, and other variables that affect land …

The Association Between Rape Myths And Continued Contact With The Perpetrator Following Sexual Abuse, Jainee Smith

Student Theses

Sexual violence is a widespread phenomenon. The term rape myth was first coined in 1980 by Martha Burt, and she defined it as “prejudicial, stereotyped, or false beliefs about rape, rape victims, and rapists” (Burt, 1980 p. 217). These myths, as well as any continued contact after a sexual assault, often lead to victim blaming, making it more difficult for victims to report any sexual assault. While rape myths were developed based on adult sexual assault, some research suggests that they also applied to child sexual abuse. This study will explore and expand the literature on rape myth acceptance and …

The Juris Master: A Proposal For Reducing Excessive Public Defender Caseloads, Blake Comeaux

Senior Honors Papers / Undergraduate Theses

The US public defense system is underfunded, understaffed, and underdelivering on the Constitutional promises of the 6^th Amendment, the right to a fair and speedy trial. This state of our public defense system results in monstrous impacts for indigent defendants nationwide. Through indefinite delays in litigation, being abandoned in jail while sitting on waiting lists for public defenders, and being outright denied representation, indigent defendants are deprived of their rights. Beyond just defendant neglect, our current system puts immense strain on public defenders, prosecutors, and state budgets. In an attempt to combat this current state of affairs, this paper …

Ndls Communicator: Week Of 05.15.23, Notre Dame Law School

NDLS Communicator

The Latest News

• ND Law’s 2023 Shaffer Fellows to address housing issues, provide expungement relief in Chicago and rural Kentucky
• Justice Leona Theron of the Constitutional Court of South Africa visits Notre Dame Law School
• ND Law’s Religious Liberty Clinic represents Sikh, Jewish, and Muslim groups defending a Muslim inmate’s religious rights
• 3L Erica Patterson named ABA Law Student Representative of the Year
• Studying abroad – in America: Japanese prosecutor at Notre Dame Law to learn about American law
• Marcus Cole was one of four keynote speakers who provided remarks at the Fairness for All Summit marking the 10th annual …

Negotiated Success: Contractual Benefits That Enhance Recruitment And Retention, Hella Bel Amor, Staci Corey, Dayna Jean Defeo

CAEPR Reports

This report summarizes monetary and non-monetary items used in Collectively Bargained Agreements (CBAs) to enhance retention and recruitment of educators in and outside of Alaska. This report is one of a series commissioned by the Alaska Department of Education and Early Development to support a stakeholder-informed action plan to address the state’s recurring critical challenges in recruiting and retaining teachers. To approach this task, we narrowed our focus to a review of provisions contained within CBAs in Alaska and a sample of districts in the nation, reviewed relevant literature, and collected stakeholder feedback to further inform the report content and …

Ragd Auto-Activating Mutations Impair Mit/Tfe Activity In Kidney Tubulopathy And Cardiomyopathy Syndrome, Irene Sambri, Marco Ferniani, Giulia Campostrini, Marialuisa Testa, Viviana Meraviglia, Mariana E G De Araujo, Ladislav Dokládal, Claudia Vilardo, Jlenia Monfregola, Nicolina Zampelli, Francesca Del Vecchio Blanco, Annalaura Torella, Carolina Ruosi, Simona Fecarotta, Giancarlo Parenti, Leopoldo Staiano, Milena Bellin, Lukas A Huber, Claudio De Virgilio, Francesco Trepiccione, Vincenzo Nigro, Andrea Ballabio

Duncan NRI Faculty and Staff Publications

Heterozygous mutations in the gene encoding RagD GTPase were shown to cause a novel autosomal dominant condition characterized by kidney tubulopathy and cardiomyopathy. We previously demonstrated that RagD, and its paralogue RagC, mediate a non-canonical mTORC1 signaling pathway that inhibits the activity of TFEB and TFE3, transcription factors of the MiT/TFE family and master regulators of lysosomal biogenesis and autophagy. Here we show that RagD mutations causing kidney tubulopathy and cardiomyopathy are "auto- activating", even in the absence of Folliculin, the GAP responsible for RagC/D activation, and cause constitutive phosphorylation of TFEB and TFE3 by mTORC1, without affecting the phosphorylation …

Loneliness And Social Networks Of Older Adults In Rural Communities: A Narrative Synthesis Systematic Review, Basharat Hussain, Mahrukh Mirza, Rebecca Baines, Lorna Burns, Sebastian Stevens, Sheena Asthana, Arunangsu Chatterjee

Peninsula Medical School

Introduction: Loneliness has significant impacts on the health of older adults. Social networks help to improve psychosocial and quality of life outcomes among older adults. A fifth of older adults live in rural communities, where geographic isolation poses challenges to health. The dynamics of social networks of older adults in rural communities has not been explored. Objective: To synthesize the literature related to loneliness and social networks of older adults in rural settings. Methods: A mixed-methods systematic review was conducted through searching six bibliographic databases to retrieve peer-reviewed literature with no time limits. We performed a methodological assessment of each …

Evaluating The Potential Of Geopolymer Concrete As A Sustainable Alternative For Thin White-Topping Pavement, Sathvik S. Chandra, Pshtiwan Shakor, Sarwar Mohmmad, Bankole O. Awuzie, Atul K. Singh, Abishek Rauniyar, M Karakouzian

Civil and Environmental Engineering and Construction Faculty Research

Introduction:

The construction industry uses a large quantity of natural materials in the production of concrete. Although attempts to incorporate green materials in concrete began years ago, not every building uses such materials today, and roadways, particularly, still rely on unsustainable materials.

Methods:

Therefore, this study used alternative materials, including fly ash, manufactured sand aggregates, and different molarities of alkaline activators, to incorporate waste byproducts in a geopolymer concrete white-topping pavement layer. Recent developments have led to the emergence of geopolymers as distinct classes of materials. In the 1990s, fly ash-based geopolymers became more popular than other kinds, as they …

Unraveling The Situation Of Women In Stem Areas From The European Teacher Perspective: Insights From Females Project, Ayşin Kaplan Sayı, Nihal Yurtseven, Şirin Karadeniz, Sinem Vatanartıran

The Qualitative Report

The purpose of this study is to examine the views of teachers on the number and situation of women in STEM areas as well as ways to encourage female students to STEM areas. We carried out the study through basic qualitative research. The participants of the study included 39 teachers from Turkey, Italy, Spain, Greece, and Romania. For data collection, focus group interviews were carried out in each country. The collected data were analyzed through content analysis. The study yielded similar findings in almost all the countries showing that the number of women was not sufficient, and the participants mostly …

Downstream Gradients In Unit Stream Power Influence Log Jam Location And Process Domain, Eliza H. Malakoff

Dartmouth College Master’s Theses

Growing calls for the use of natural materials and processes to meet management goals have positioned artificial log jams as a compelling alternative to hard engineering instream and floodplain habitat. Deep uncertainties remain, however, about where and how wood should be placed to best mimic natural river processes. In this study, I test whether at-a-point or downstream gradients in unit stream power, an estimate of a river’s ability to do work, exert control over where and how log jams form. Using field observations of 360 log jams in New Hampshire and Vermont and an additional 320 previously published locations of …

Re-Membering The Living Earth: A Year In Rural Sri Lanka, Samuel C. King

Dartmouth College Master’s Theses

The following thesis tells the story of my year in rural Sri Lanka. After college, I traveled from suburban New York to the highlands of the island country with the hopes of writing an ethnography on agrarian Buddhism. I soon realized, however, that I was not just embarking on an academic project, but an inner journey to explore ways of being that had been lost in the modern culture I had known. My narrative recounts how immersion in a rice cultivating village deepened my sense for what it means to live in reciprocity with the more-than-human world—a world of plants, …

Placing A Bid: A Comparison Of The Traditional Marketplace (Stocks) And Non-Traditional Markets (Nfts), Nieh, Haley

Santa Clara High Technology Law Journal

Non-fungible Tokens (NFTs) are exploding in the marketplace and are not losing momentum anytime soon. Artists, athletes, celebrities, and even brands and luxury houses are rolling out NFTs. With this excitement, a great deal of profit is being generated; the market cap of NFTs is expected to grow from $3 billion in 2022 to $13.6 billion in 2027 (a compound annual growth rate of 35 percent).1

Blockchain technologies are an empowering platform for democratization of financial instruments and transactions. Cryptocurrency has received some regulation from the United States Securities and Exchange Commission (SEC), but clarity and regulation of NFTs have …

Birthing Reimagined: Perceptions Of Safety And Autonomy In Birth Experiences Among Postpartum Individuals During Covid-19, Beldina Orinda

Senior Honors Papers / Undergraduate Theses

Background: Safety during childbirth is a priority for care providers, but patients' perceptions of safety may differ from those of providers, especially in biomedical settings.1–4 This can influence health outcomes and satisfaction with the birth experience.5–7 We hypothesize that autonomy, defined as the ability to make decisions about care during childbirth, may enhance feelings of safety and positive mental health outcomes.9,15

Methods: Surveys via REDCap v11.1.29 captured the experiences and Edinburgh Postnatal Depression Scale (EPDS) scores of pregnant and postpartum women. Participants (n=49) included 15 pregnant and 34 postpartum individuals, aged 18 and older, who had given …

The Fugazi Second Amendment: Bruen's Text, History, And Tradition Problem And How To Fix It, Patrick J. Charles

Cleveland State Law Review

This Article critiques the Supreme Court’s use of text, history, and tradition in New York Rifle & Pistol Association, Inc. v. Bruen. In doing so, not only is the Supreme Court’s approach to history-in-law in Bruen called into question, but also the Article provides the courts with an historically objective and even-keeled ‘way-ahead’ for future Second Amendment cases and controversies.