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The Cost Of Cargo Preference: How Shipping Requirements Hinder Food Aid Efficiency, Grace E. Pattara Apr 2026

The Cost Of Cargo Preference: How Shipping Requirements Hinder Food Aid Efficiency, Grace E. Pattara

Senior Honors Theses

Cargo preference for food aid (CPFA) mandates that 50 percent of U.S. food aid shipments be transported on U.S.-flagged vessels (USFVs). However, only seven USFVs are equipped to accommodate bulk food aid, causing shipments to be sent on vessels not designed for such transport. This constraint increases operational costs by an average of 270 percent, delays delivery timelines, and consumes 30-50 percent of the food aid budget. As the American legislature stands in an important moment in foreign aid history when U.S. foreign assistance is being fundamentally restructured, this thesis argues that Congress should repeal CPFA and permit competitive bidding …


Evaluation Of The Prevention And Management Of Assaultive Behaviors (Pmab) Training For Outpatient Ambulatory Care Staff, Michaela Bowles Apr 2026

Evaluation Of The Prevention And Management Of Assaultive Behaviors (Pmab) Training For Outpatient Ambulatory Care Staff, Michaela Bowles

Doctor of Nursing Practice

Background: Workplace violence (WPV) disproportionately affects healthcare workers and harms both staff well-being and the healthcare organization. Despite growing attention to WPV prevention, effective solutions remain limited. The purpose of this project was to evaluate the Prevention and Management of Assaultive Behaviors (PMAB) training in an outpatient ambulatory care setting and to assess staff’s confidence and ability to respond to escalating situations. Methods: A pre/post observational survey using Likert-scale, multiple choice, yes/no questions and narrative responses measured confidence before, immediately after, and 3 to 12 months following PMAB training. A retrospective review of WPV incident reports three months before and …


How Media Framing Impacts Juror Bias In A Fictional Criminal Case, Teah Sharon Swartzon Apr 2026

How Media Framing Impacts Juror Bias In A Fictional Criminal Case, Teah Sharon Swartzon

Journalism

This study examines how media framing influences juror bias among college-aged students when evaluating a defendant's guilt in a fictional criminal case. Utilizing framing theory, the research explores whether biased news coverage can shape perceptions of a defendant's guilt before trial.

Participants, consisting of students at California Polytechnic State University, aged 18-22, were asked to read two news articles about a fictional criminal case. One framed the fictional defendant as guilty, while the other framed him as innocent. Both articles were generated using the Artificial Intelligence Platform, Claude.

Data was collected in a survey where participants indicated whether they believed …


The Impact Of Corporate Venture Capital On Financing And Innovation In Academic Entrepreneurial Firms, Tingting Luo Apr 2026

The Impact Of Corporate Venture Capital On Financing And Innovation In Academic Entrepreneurial Firms, Tingting Luo

Dissertations and Theses Collection (Open Access)

Amid the ongoing advancement of China’s innovation-driven development strategy, the effective transformation of scientific research into industrial impact has become a central concern. Corporate Venture Capital (CVC), as a strategic investment mechanism connecting industrial capital with entrepreneurial ventures, is increasingly viewed as a critical external resource for high-technology start-ups. At the same time, Academic Entrepreneurship Firms (AEFs), characterized by their deep research capabilities and original technological trajectories, play a distinctive role as knowledge suppliers in uncertain, emerging industries. However, whether AEFs can effectively attract CVC support and convert it into innovation performance remains an open empirical and theoretical question.

This …


The Determinants And Consequences Of Green Credit In Commercial Banks: A Dual-Pillar Perspective Of Asset Quality And Esg, Jianmei Xia Apr 2026

The Determinants And Consequences Of Green Credit In Commercial Banks: A Dual-Pillar Perspective Of Asset Quality And Esg, Jianmei Xia

Dissertations and Theses Collection (Open Access)

As China’s “dual carbon” strategy advances, green credit has increasinglybecome a crucial financial instrument driving the green transition of China’s industrial structure. From a dual perspective of asset quality and ESGperformance, this paper systematically examines the determinants andconsequences of green credit practices among commercial banks. Employing a multiple-method approach that combines comparative case study, in-depthinterview, and large-sample empirical analysis, this study aims to address twocore questions: First, what are the key factors driving commercial banks toprovide green credit, and what is the logic behind their differentiated decision- making? Second, how does green credit affect banks’ financial and non-financial performance? This …


Enhancing Market Acceptance Of Sports Rehabilitation Services: A Perspective Of Public Health Literacy And Information Acquisition, Na Li Apr 2026

Enhancing Market Acceptance Of Sports Rehabilitation Services: A Perspective Of Public Health Literacy And Information Acquisition, Na Li

Dissertations and Theses Collection (Open Access)

This article takes public health literacy as its point of departure, constructs a theoretical model of “Health Literacy–Health Information Acquisition–Market Acceptance”, and introduces rehabilitation experience and service accessibility as moderating variables, in order to systematically examine the formation pathways and underlying mechanisms of market acceptance in the sports rehabilitation service sector.

This study adopts a combination of questionnaire survey and empirical analysis, collecting data from public samples across multiple regions and conducting statistical tests. The findings indicate that, Firstly, public health literacy has a statistically significant positive influence on the market acceptance of sports rehabilitation services. As the level of …


The Impact Of Mortality Awareness On Task And Relationship Leadership Styles, Sang Yeol Lee Apr 2026

The Impact Of Mortality Awareness On Task And Relationship Leadership Styles, Sang Yeol Lee

Dissertations and Theses Collection (Open Access)

In current organizational management practice, the question of how leaders can transform the universal awareness of human finitude into constructive leadership is an issue that embodies both profound humanistic concern and significant managerial implications. Based on transcendence management theory and leadership style theory, this study explores how mortality awareness affects leaders’ task orientation and relationship orientation styles, revealing its underlying mechanisms and boundary conditions. By conducting a three-wave, matched-pair questionnaire survey involving 203 enterprise managers and their subordinates, the theoretical hypotheses were tested. The findings are as follows: First, mortality awareness has a significant direct positive effect on both task-oriented …


From Ancient Harmony To Modern Sustainability: Assessing The Coupling Of Taoist “Harmony Between Humanity And Nature” With Esg And Its Impact On Firm Innovation And Performance, Biyi Wang Apr 2026

From Ancient Harmony To Modern Sustainability: Assessing The Coupling Of Taoist “Harmony Between Humanity And Nature” With Esg And Its Impact On Firm Innovation And Performance, Biyi Wang

Dissertations and Theses Collection (Open Access)

Against the backdrop of intensifying global ecological crises, the ESG evaluation system has become a core metric for corporate sustainable development worldwide. However, the Western-dominated ESG system lacks crucial cultural dimensions in its application within Chinese corporate practices. The ecological wisdom embedded in the Taoist concept of “Harmony between Humanity and Nature” offers a significant philosophical foundation for the ESG localization. Extant research has focused on the influence of Confucian culture on ESG, with insufficient exploration of the modern managerial value of “Harmony between Humanity and Nature”. A scientific coupling framework linking this concept with ESG indicators has yet to …


Determinants Of Evaluation Demand In The United States Federal Government: A Sequential Mixed Methods Study With Hierarchical Linear Modeling, Ruqayyah Abu-Obaid Apr 2026

Determinants Of Evaluation Demand In The United States Federal Government: A Sequential Mixed Methods Study With Hierarchical Linear Modeling, Ruqayyah Abu-Obaid

Dissertations

This dissertation examines the factors that influence demand for evaluation in the federal government of the United States. Although evaluation has become a central element of evidence-based policymaking, limited empirical research explains what drives variation in evaluation activity across federal agencies. The study addresses this gap through a sequential exploratory mixed-methods design that integrates qualitative document analysis with quantitative hierarchical (panel) data modeling. Hierarchical linear modeling (HLM) was employed to analyze longitudinal data from 23 federal agencies (2008–2024), accounting for both within- and between-agency variation over time and providing more accurate estimates of factors influencing evaluation demand.

In the qualitative …


Patenting The Unexpected, Sean B. Seymore Apr 2026

Patenting The Unexpected, Sean B. Seymore

Vanderbilt Law School Faculty Publications

Unexpected discoveries have transformed human existence. They’ve revolutionized science and spawned entire new fields of research. While unexpected discoveries seem inventive and worthy of patent protection, they don’t formally align with traditional patent theory. The bedrock principle that an invention begins with the conception of an idea in the inventor’s mind and ends with implementation doesn’t apply to unexpected discoveries. This misalignment is most acute for pharmaceuticals, where the discovery of new uses for old drugs has tremendous social and economic importance. Since a drug can inherently treat multiple conditions in a patient—even those unknown to the patient, doctor, or …


Paraeducators' Implementation Fidelity Of Functional Communication Training Following An Intervention Package Of Behavior Skills Training And ECoaching, Autumn M. Alexander Apr 2026

Paraeducators' Implementation Fidelity Of Functional Communication Training Following An Intervention Package Of Behavior Skills Training And ECoaching, Autumn M. Alexander

Human Movement Studies & Special Education Theses & Dissertations

My first purpose of this study was to determine if an intervention package of Behavior Skills Training (BST) and eCoaching was effective in increasing and maintaining paraeducator implementation fidelity when implementing Functional Communication Training (FCT) with students with multiple disabilities. Second, I sought to measure the effectiveness of FCT on three students’ challenging behavior and a functional communicative response (FCR). Third, I collected information from paraeducators and teachers about the perceptions of the training methods and FCT for practical usage in the classroom. I used a multiple-probe research design across three paraeducator and student dyads and demonstrated that the intervention …


An Integrated Bayesian Network-Based Zero Trust Model To Quantify Cyber Risk In Small-Medium Businesses, Ahmed Abdelmagid Apr 2026

An Integrated Bayesian Network-Based Zero Trust Model To Quantify Cyber Risk In Small-Medium Businesses, Ahmed Abdelmagid

Engineering Management & Systems Engineering Theses & Dissertations

Small-medium businesses (SMBs) play a pivotal role in the worldwide economy as they constitute the most considerable portion of businesses in developed countries like the UK and the US. As such, SMBs are likely targets of cybercrimes by malicious agents because of their vulnerable IT systems. The digital infrastructure of SMBs is more likely to be hit by cyberattacks than large businesses due to many factors that facilitate hackers’ missions. These factors include a limited financial budget devoted to cybersecurity, a lack of knowledge, an underrating of how dangerous cyber threats are, and a shortage of IT expertise. The enormous …


Optimization Of Niobium Film For Particle Accelerators And Quantum Applications, Bektur Abdisatarov Apr 2026

Optimization Of Niobium Film For Particle Accelerators And Quantum Applications, Bektur Abdisatarov

Electrical & Computer Engineering Theses & Dissertations

Niobium (Nb) films play a central role in superconducting technologies used in particle accelerators and superconducting quantum circuits. Optimizing the physical properties of Nb films is therefore critical for improving both radiofrequency (RF) performance in superconducting radiofrequency (SRF) cavities and coherence in superconducting qubits. This thesis investigates the relationship between Nb film microstructure, impurity content, and electromagnetic response across these two application domains.

For particle accelerator applications, we studied Nb films deposited using high-power impulse magnetron sputtering (HiPIMS) with DC bias onto a 1.3 GHz elliptical SRF cavity. Nb film cavities exhibit a pronounced medium-field Q-slope, limiting their achievable accelerating …


Radicals Transforming Organic Matter: Implications For Non-Pyrogenic Black Carbon And Nitrogen Formation, Molecular Dynamics In Tropical Soils, And Lignin Valorization, João Vitor Dos Santos Apr 2026

Radicals Transforming Organic Matter: Implications For Non-Pyrogenic Black Carbon And Nitrogen Formation, Molecular Dynamics In Tropical Soils, And Lignin Valorization, João Vitor Dos Santos

Chemistry & Biochemistry Theses & Dissertations

Radicals drive the transformation of organic matter in natural and engineered systems, altering its structure, reactivity, and stability. Condensed aromatic carbon (ConAC) and condensed aromatic nitrogen (ConAN) are among the most persistent forms of organic matter in soils and are traditionally interpreted as products of biomass burning. This dissertation challenges the long-standing assumption that these materials are exclusively pyrogenic, showing that iron-mediated radical chemistry can generate condensed aromatic structures under ambient conditions.

Chapters II and III show that Fenton-like radical oxidation of lignin-rich biomass produces condensed aromatic structures compositionally similar to pyrogenic black carbon and black nitrogen when assessed with …


Quantifying Nutrient Fluxes Across The Sediment-Water Interface In The Lafayette River Estuary, Adriana E. Amrhein Apr 2026

Quantifying Nutrient Fluxes Across The Sediment-Water Interface In The Lafayette River Estuary, Adriana E. Amrhein

OES Theses and Dissertations

The Lafayette River, a shallow, eutrophic tributary of the Chesapeake Bay, experiences seasonal harmful algal blooms (HABs). While management efforts focus on reducing external nutrient inputs, internal loading from benthic sediments remains poorly constrained. This study quantifies spatial and seasonal variability in nutrient and radium isotope fluxes across the sediment–water interface at three sites where HABs are known to initiate and represent contrasting sediment textures and hydrodynamic conditions. Sediment cores and porewater samples were collected in summer 2023 and winter 2024 to measure grain size, porosity, dissolved nutrients (NH₄⁺, NOₓ⁻, PO₄³⁻), and radionuclide activities (²²⁴Ra and ²²⁸Th). Radium isotope disequilibrium …


Social Capital As An Intervening Explanatory Mechanism Linking Socioeconomic Status And Deliberative Decision-Making In Citizen Advisory Boards In California Municipalities, Christopher Gerry Apr 2026

Social Capital As An Intervening Explanatory Mechanism Linking Socioeconomic Status And Deliberative Decision-Making In Citizen Advisory Boards In California Municipalities, Christopher Gerry

School of Public Service Theses & Dissertations

Citizen advisory boards (CABs) are widely used in local government as institutional mechanisms for participatory governance and public input. While these bodies are intended to promote inclusive and deliberative decision-making, persistent socioeconomic inequalities raise concerns about whether CABs mitigate or reproduce disparities in participation and influence. This dissertation examines how socioeconomic status (SES) shapes deliberative decision-making within CABs and whether this relationship operates indirectly through board-level social capital.

Integrating perspectives from participatory governance, deliberative democracy, and social capital theory, the study conceptualizes deliberative decision-making as a collective, board-level process shaped by relational and institutional dynamics rather than individual attributes alone. …


A Theoretical Framework For Recursion In Complex System Governance, Gilbert Goddin Apr 2026

A Theoretical Framework For Recursion In Complex System Governance, Gilbert Goddin

Engineering Management & Systems Engineering Theses & Dissertations

The purpose of this research was to construct an original theoretical framework for understanding recursion in Complex System Governance (CSG), grounded in the propositions and axioms of systems theory and management cybernetics. This framework addresses a research gap for cybernetic recursion relating to the governance of complex systems. Leveraging the CSG framework (Keating & Katina, 2019; Keating, 2022), holonic principles (Koestler, 1970; Mella, 2009), management cybernetics (Beer, 1979; Beer, 1984; Beer, 1995), and metacybernetics (Yolles, 2021; Yolles & Fink, 2015c; Yolles & Frieden, 2021), recursion is theorized as a governable capability, whereby metasystemic governance anticipates emerging complexity and purposefully regulates …


Women's Displacement And The Reclamation Of Identity: Navigating Landscapes Of Contestation In The Postcolonial Novel, Carol P. Harvest Apr 2026

Women's Displacement And The Reclamation Of Identity: Navigating Landscapes Of Contestation In The Postcolonial Novel, Carol P. Harvest

English Theses & Dissertations

Landscapes affected by colonial domination are contested landscapes—places not only in danger of environmental destruction through colonial and neocolonial violence but also landscapes of struggle for people who survive in these precarious spaces. Historically, separate postcolonial and ecocritical literary analysis addressed contested landscapes; postcolonial critique centered on the lasting devastation of colonial violence on people and place, while ecocriticism, in its infancy, enjoyed the privilege of ignorance, focusing on endangered species and the preservation of land, but it did not consider the impact of landscapes on the very people who were largely forced to either leave or cross “natural” spaces. …


Cultural Memory And Colonial Legacies: The Pandemic Era In France, Kathryn (Kate) R. Cross Apr 2026

Cultural Memory And Colonial Legacies: The Pandemic Era In France, Kathryn (Kate) R. Cross

Graduate Program in International Studies Theses & Dissertations

In the twenty-first century, how are former colonial powers addressing colonial legacies? Amidst the massive social movements of the pandemic years and greater representations of a new generation of politicians in major elections, the global zeitgeist was increasingly characterized by representation of disenfranchised and previously silenced memories and narratives. These shifts, especially in France, are a sharp contrast to the unofficial historic policy of amnesia maintained regarding traumatic legacies and build on movements that began at the turn of the century. The environment has facilitated an increased ability for cultural products to create, shape, and sustain cultural memories of colonial …


Sequencing Dengue Control Policy In Singapore: An Evolutionary Perspective For Policy Design, Ishani Mukherjee, Panchali Guha Apr 2026

Sequencing Dengue Control Policy In Singapore: An Evolutionary Perspective For Policy Design, Ishani Mukherjee, Panchali Guha

Research Collection School of Social Sciences

About half the global population is now at risk of contracting dengue, a mosquito-borne viral infection that can cause severe morbidity and fatalities. Effective dengue control depends on controlling the mosquito vector, but finding the right mix of vector control policies has proved challenging. Using a content analysis of 208 Hansard records from parliamentary proceedings in Singapore, where dengue outbreaks have significantly increased in both frequency and magnitude since the early 1990s, we trace the processual evolution of Singapore’s anti-dengue policy mix from 1960 to 2023 and conclude that the evolution of dengue control policies is consistent with a customized …


Who You Explain To Matters: Learning By Explaining To Conversational Agents With Different Pedagogical Roles, Zhengtao Xu, Junti Zhang, Anthony Tang, Yi-Chieh Lee Apr 2026

Who You Explain To Matters: Learning By Explaining To Conversational Agents With Different Pedagogical Roles, Zhengtao Xu, Junti Zhang, Anthony Tang, Yi-Chieh Lee

Research Collection School Of Computing and Information Systems

Conversational agents are increasingly used in education for learning support. An application is “learning by explaining”, where learners explain their understanding to an agent. However, existing research focuses on single roles, leaving it unclear how different pedagogical roles influence learners’ interaction patterns, learning outcomes and experiences. We conducted a between-subjects study (N=96) comparing agents with three pedagogical roles (Tutee, Peer, Challenger) and a control condition while learning an economics concept. We found that different pedagogical roles shaped learning dynamics, including interaction patterns and experiences. Specifically, the Tutee agent elicited the most cognitive investment but led to high pressure. The Peer …


A Culture Divided: How Irish Americans Responded To Ireland’S Wartime Neutrality, Robert J. Barron Apr 2026

A Culture Divided: How Irish Americans Responded To Ireland’S Wartime Neutrality, Robert J. Barron

Honors College Theses

In the Second World War, the nation of Ireland declared its neutrality and maintained this status through the course of the long conflict. Meanwhile, Germany, Great Britain, and eventually the United States all competed to gain Ireland’s support in the war. However, one overlooked group continued to keep tabs on the affairs within their homeland: Irish Americans. How does one respond to a nation you have a connection with staying neutral in such a deadly conflict? The answer, for Irish Americans, was that it depended on the year. Before the United States entered the war, Irish Americans firmly defended the …


Probing The Prevalence Of Pharmaceutical Corruption, Marc A. Rodwin Apr 2026

Probing The Prevalence Of Pharmaceutical Corruption, Marc A. Rodwin

Suffolk University Law School Faculty Works

This article reviews the history and current evidence of systematic pharmaceutical industry corruption.  It draws on studies by the OECD on international corruption; reports of the Securities and Exchange Commission; studies of the Public Citizen health Research Group of settlement agreements between federal and state authorities and pharmaceutical firm; the history of the Medicare and Medicaid Anti-Kickback Act; professional and industry codes of ethics; the literature on institutional corruption; and studies of conflicts of interest.  These sources support findings of systemic corruption since the mid-20th century. The paper also explores the relationship between classic corruption, institutional corruption and conflicts of …


Evolution Of Antibiotic Responses During Chronic P. Aeruginosa Infections In The Human Lung, David Frank Ritz Apr 2026

Evolution Of Antibiotic Responses During Chronic P. Aeruginosa Infections In The Human Lung, David Frank Ritz

Dartmouth College Ph.D Dissertations

Typical antibiotic susceptibility testing (AST) of microbial samples is performed in homogeneous cultures in batch environments, which does not account for the highly heterogeneous and dynamic nature of antibiotic responses. The most common mutation found in P. aeruginosa lineages evolved during chronic cystic fibrosis infections in the human lung, a loss of function of repressor MexZ, increases basal levels of multidrug efflux MexXY, but does not increase resistance by traditional minimal inhibitory concentration (MIC) assays. Here, we use single cell microfluidics to show that P. aeruginosa’s response to aminoglycosides is highly heterogeneous, with only a subpopulation of cells surviving exposure. …


False Conflict: Colorblindness And Section 2 Of The Voting Rights Act, W. Kerrel Murray Apr 2026

False Conflict: Colorblindness And Section 2 Of The Voting Rights Act, W. Kerrel Murray

Faculty Scholarship

If friends and foes of section 2 of the Voting Rights Act of 1965 agree on one thing, it is the irrepressible conflict between section 2’s attention to race and constitutional colorblindness.  They’re wrong.  Rightly understood, section 2’s design is remarkably compatible with colorblind constitutionalism’s letter and spirit.

To show how, this Article first returns to section 2’s origins and the preexisting approach its text codifies.  That approach had an underappreciated ethos:  When intentional discrimination’s effects sufficiently distort a political system, an electoral rule’s results might impugn its unconstitutionality, even if the rule had been adopted without discriminatory purpose.  Drawing …


How To Transform Capitalism Through Law, Katharina Pistor Apr 2026

How To Transform Capitalism Through Law, Katharina Pistor

Faculty Scholarship

This essay is based on the presentation of my book, The Law of Capitalism and How to Transform It, at the University of Miami Law School on October 14, 2025. I am grateful for the opportunity to share a summary of this work in the University of Miami Law Review and to my colleagues Ingo Venzke and Martijn Hesselink, who were kind enough to offer their thoughts on it for this mini symposium.


Agenda Control And Precedent, Thomas P. Schmidt Apr 2026

Agenda Control And Precedent, Thomas P. Schmidt

Faculty Scholarship

The U.S. Supreme Court now has vast control over its agenda: It has the power to pick and choose not only what cases it will hear through writs of certiorari, but also what specific legal questions within those cases it will consider. As a result, the Court never revisits a precedent unless it has made a prior, discretionary choice to put the viability of a precedent on its agenda. For that reason, the Court’s powers of agenda control play a significant and underappreciated role in maintaining the continuity of the legal order. Indeed, it is no surprise that the formal …


Thinking Constructively About International Law, Monica Hakimi Apr 2026

Thinking Constructively About International Law, Monica Hakimi

Faculty Scholarship

The international order that has defined the post-World War II period is being radically transformed, presenting a once-in-a-generation opportunity to reconstitute its basic foundations. The pressing questions for international lawyers are about the roles their enterprise can and should play going forward. I argue in this Article that most lack the analytic framework they need even to begin tackling these questions. Worse, most seem mired in an old theory about law that corrupts how they approach their enterprise. My goals in the Article are to expose the prevalence of this bad theory and to present the grounds for discarding it, …


Uncomfortably Reminiscent: Odonnell V. Harris County In History And Memory, Kellen R. Funk Apr 2026

Uncomfortably Reminiscent: Odonnell V. Harris County In History And Memory, Kellen R. Funk

Faculty Scholarship

In ODonnell v. Harris County, a federal trial court enjoined the misdemeanor bail system of Houston, Texas, freeing approximately 20,000 indigent defendants annually. To do this, the trial court drew upon Reconstruction era precedents establishing intensive federal oversight of state criminal proceedings. The court explicitly invoked the Civil War and Reconstruction transformation of the federal judiciary, comparing the County’s defenses to slavery era arguments. Conversely, when the en banc Fifth Circuit reviewed a similar order in Dallas, Texas, six years later, it rejected this historical framework, condemning such litigation as an impermissible “ongoing federal audit of state criminal proceedings.” …


Tfeb Degradation Is Regulated By An Ikk/Β-Trcp2 Phosphorylation-Ubiquitination Cascade, Yan Xiong, Jaiprakash Sharma, Meggie N Young, Wen Xiong, Ali Jazayeri, Karl F Poncha, Ma Xenia G Ilagan, Qing Wang, Bei Gao, Hui Zheng, Nicolas L Young, Marco Sardiello Apr 2026

Tfeb Degradation Is Regulated By An Ikk/Β-Trcp2 Phosphorylation-Ubiquitination Cascade, Yan Xiong, Jaiprakash Sharma, Meggie N Young, Wen Xiong, Ali Jazayeri, Karl F Poncha, Ma Xenia G Ilagan, Qing Wang, Bei Gao, Hui Zheng, Nicolas L Young, Marco Sardiello

Center on Aging Staff Publications

Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and cellular clearance pathways. TFEB activity is tightly controlled by multiple post-translational mechanisms, but the exact molecular mechanism controlling its stability has remained elusive. Here, we identify the IκB kinase (IKK) complex as a key regulator of TFEB protein stability through a phosphorylation-ubiquitination cascade. A high-content kinase inhibitor screen reveals that IKK inhibition increases TFEB protein levels, and genetic ablation of IKK components increases TFEB stability, upregulates lysosomal genes, and enhances lysosomal biogenesis and degradative capacity. Mechanistically, we show that IKK phosphorylates TFEB on a cluster of serine residues …