Digital Commons Network^™

A Human Mitofusin 2 Mutation Can Cause Mitophagic Cardiomyopathy, Antonietta Franco, Jiajia Li, Daniel P Kelly, Ray E Hershberger, Ali J Marian, Renate M Lewis, Moshi Song, Xiawei Dang, Alina D Schmidt, Mary E Mathyer, John R Edwards, Cristina De Guzman Strong, Gerald W Dorn

2020-Current year OA Pubs

Cardiac muscle has the highest mitochondrial density of any human tissue, but mitochondrial dysfunction is not a recognized cause of isolated cardiomyopathy. Here, we determined that the rare mitofusin (MFN) 2 R400Q mutation is 15-20× over-represented in clinical cardiomyopathy, whereas this specific mutation is not reported as a cause of MFN2 mutant-induced peripheral neuropathy, Charcot-Marie-Tooth disease type 2A (CMT2A). Accordingly, we interrogated the enzymatic, biophysical, and functional characteristics of MFN2 Q400 versus wild-type and CMT2A-causing MFN2 mutants. All MFN2 mutants had impaired mitochondrial fusion, the canonical MFN2 function. Compared to MFN2 T105M that lacked catalytic GTPase activity and exhibited normal …

A Consortium Of Microalgae And Wastewater Activated Sludge To Generate Biohydrogen, Muhammad Asad Javed

Dissertations

Hydrogen is a clean source and carbon-free source of energy that can be generated photobiologically using green microalgae with 122 kJ/g of energy output which is 2.75 times that of hydrocarbon-based fuels. However, challenges, such as presence of molecular oxygen (O₂) within the cells, hinder their practical application. Molecular O₂, emanating from the activity of photosynthesis, acts as a powerful and effective switch that turns off bioH₂ production activity. The objective of this research was to develop an innovative approach to increase the yield and sustain bioH₂ photoproduction. For this purpose, a consortium of …

Our Authors

Gathered Fragments

No abstract provided.

Pedagogy In Times Of Crisis, James Daria, Abigail Wightman, Shelly Yankovskyy, Amanda J. Reinke

Southern Anthropologist

Editors’ note: With this issue, we launch a new feature of the journal, drawing from a panel discussion or roundtable at SAS, which sparked important discussion for panelists and conference participants. This panel, which took place on April 9, 2022, in Raleigh, NC, was part of the 55th Annual Meeting of the Southern Anthropological Society with the theme “Public Interest and Professional Anthropology in the South.” The roundtable was organized and moderated by Amanda J. Reinke. The transcript was created by Helen Regis and the conversation was lightly edited by the authors, who also had an opportunity to include references …

Building The Soil Carbon Sponge At Murray Wells Farm, Tambellup, Western Australia, Wendy Bradshaw

Natural resources published reports

Peter and Wendy Bradshaw farmed at Murray Wells Farm about 25 km west of Tambellup for around 46 years until they sold and retired in 2022. They produced barley, oats, merino and prime lambs on an annual rainfall of 450 mm over 1,000 arable hectares. The farm was on a moderately undulating landscape with a mix of soils including sandy duplex, well-drained sand over gravel over clay, shallow sandy or loamy gravel over cemented laterite and red to brown loamy soils.Their farming philosophy was to view agriculture as an ecological enterprise that included them as part of the ecosystem, and …

Challenges And Responses : The Impact Of Classification Methods Of Policies On First-Generation College Students In China And The Us, Ken Cheng

SOC 605 Comparative Social Policy Research Project - Student Works

In China and the United States, with the expansion of higher education, more and more people have become the first-generation of college students in their families. However, while gaining opportunities for social upward mobility, they also face many challenges, and the formation of these challenges is related to their multiple identities. In China, these challenges are more related to regional development differences, while in the United States, they are more impacted by identities such as immigrants and ethnic minorities. At the same time, the governments, universities, and society of both countries have introduced various policies to support first-generation college students, …

Citizenship Outside The Courts, Catherine Y. Kim

Faculty Scholarship

No abstract provided.

Big Boned: How Fat Storage And Other Adaptations Influenced Large Theropod Foraging Ecology, Cameron Pahl, Luis A. Ruedas

Biology Faculty Publications and Presentations

Dinosaur foraging ecology has been the subject of scientific interest for decades, yet much of what we understand about it remains hypothetical. We wrote an agent-based model (ABM) to simulate meat energy sources present in dinosaur environments, including carcasses of giant sauropods, along with living, huntable prey. Theropod dinosaurs modeled in this environment (specifically allosauroids, and more particularly, Allosaurus Marsh, 1877) were instantiated with heritable traits favorable to either hunting success or scavenging success. If hunter phenotypes were more reproductively successful, their traits were propagated into the population through their offspring, resulting in predator specialists. If selective pressure favored scavenger …

Drivers Of Plankton Community Structure In Intermittent And Continuous Coastal Upwelling Systems–From Microbes And Microscale In-Situ Imaging To Large Scale Patterns, Moritz Schmid, Su Sponaugle, Anne W. Thompson, Kelly T. Sutherland, Robert Cowen

Biology Faculty Publications and Presentations

Eastern Boundary Systems support major fisheries whose early life stages depend on upwelling production. Upwelling can be highly variable at the regional scale, with substantial repercussions for new productivity and microbial loop activity. Studies that integrate the classic trophic web based on new production with the microbial loop are rare due to the range in body forms and sizes of the taxa. Underwater imaging can overcome this limitation, and with machine learning, enables fine resolution studies spanning large spatial scales. We used the In-situ Ichthyoplankton Imaging System (ISIIS) to investigate the drivers of plankton community structure in the northern California …

Germ Cell Recovery, Cryopreservation And Transplantation In The California White Sturgeon, Acipenser Transmontanus, Amie L. Romney, Danielle M. Myers, Fatima R. Martin, Tawney N. Scanlan, Stuart A. Meyers

Biology Faculty Publications and Presentations

The white sturgeon (Acipenser transmontanus) is the largest freshwater fish in North America. Because of the unique life history characteristics of sturgeon, including longevity, late maturation and long spawning intervals, their aquaculture can be a significant investment of resources. As a result of habitat loss and overharvesting, natural populations of white sturgeon are threatened and there is a growing effort to improve conservation aquaculture programs. Germ cell transplantation is an innovative technology previously demonstrated in a variety of fish species to be able to produce a surrogate broodstock. The technique relies upon optimal donor germ cell recovery and transplantation into …

Characterization And Evaluation Of Bioactivity Of Dry Edible Bean And Bean Derived Small Peptides, Bikram Prasad Upadhyaya

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Obesity, diabetes, and their metabolic complications continue to be one of the major global public health concerns with a few effective treatment options. Although regular exercise and a well-balanced diet that includes calories from pulses can be recommended, much remains to be elucidated about the health effects of the various bioactive compounds including peptides and polyphenols present in pulses. Most of the previous studies have focused on individual dietary compounds and only a few investigated the synergistic effect of active compounds in modulating human health. Hence this dissertation aimed first, to provide a comparative evaluation of the overall therapeutic value …

Biomimetic Approach To Elucidate The Role Of Microenvironment In Regulating Cell Communications In Liver Diseases, Youra Moeun

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Liver is a complex organ but often underappreciated for its functions to remove toxins and fight infection in the body. Despite the ongoing prevalence of patients contracting chronic liver diseases, coupled with a global healthcare investment of approximately $70 billion, there is still no effective treatment for chronic liver diseases including alcoholic liver disease. The purpose of this dissertation was to understand the interactions of parenchymal and non-parenchymal liver cells in physiological and pathological like environments. This allowed us to recreate a healthy and the diseased microenvironments to observe the stages of the liver diseases development and apply appropriate treatments …

Modeling Overdraft-Driven Nitrate Transport In Shallow Wells For Mitigation And Scenario Planning, Jonathan Cronk

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

In Nebraska, average nitrate concentrations in groundwater have doubled since 1974, making water quality management more important than ever. As droughts, heat waves, and floods become more common climate events, understanding their impacts will be necessary to make informed management decisions. Emerging literature describes that drought correlates to an increase in the concentration of nitrate-N at domestic and irrigation wells, however the relative contributions of the mechanisms thought to be responsible is currently unknown.

This research assessed the impact of recharge and pumping rate changes as two mechanisms affecting nitrate-N concentration during drought, assessed the relationship between well depth and …

Serum Ferritin Values In Neonates <29 Weeks' Gestation Are Highly Variable And Do Not Correlate With Reticulocyte Hemoglobin Content, Timothy M Bahr, Sylvia Tan, Emily Smith, Sandra S Beauman, Kurt R Schibler, Cathy A Grisby, Jean R Lowe, Edward F Bell, Abbot R Laptook, Seetha Shankaran, David P Carlton, Carrie Rau, Mariana C Baserga, John Flibotte, Kristin Zaterka-Baxter, Michele C Walsh, Abhik Das, Robert D Christensen, Robin K Ohls, Eunice Kennedy Shriver National Institute Of Child Health And Human Development Neonatal Research Network

Pediatrics Research and Scholarship

OBJECTIVES: To compare serum ferritin and RET-He values among extremely low gestational age neonates ELGANs with other markers of iron-deficient erythropoiesis.

STUDY DESIGN: This is a secondary analysis of the NICHD Darbepoetin Trial. Study data from placebo recipients who had a serum ferritin, a RET-He, and a mean corpuscular volume (MCV) measurement within a 24-hour period were analyzed for correlation.

RESULTS: Mixed linear regression models showed no association between ferritin and RET-He at both early (β = 0.0016, p = 0.40) and late (β = -0.0001, p = 0.96) time points. Positive associations were observed between RET-He and MCV at …

Housing Discrimination And Negative Attitudes Towards Ex-Offender Parents, Julie C. Wertheimer-Meier

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

While the Fair Housing Act prohibits housing discrimination because of race, gender, religion, sex, disability, family status, and national origin, it allows housing providers to discriminate on the basis of criminal history. Prior research shows that housing providers disproportionately deny housing to ex-offender applicants and single parent applicants with young children. An ex-offender parent’s inability to acquire safe and affordable housing decreases the potential for reunification with their children and increases the risk of lost custody or parental rights termination. This dissertation consisted of two experiments that examined the effects of negative attitudes towards ex-offender parents on those parents’ ability …

The Application Of Electrosprayed Minocycline-Loaded Plga Microparticles For The Treatment Of Glioblastoma, Marco Antonio Arriaga, Juan A. Amieva, Jaqueline Quintanilla, Angela C. Jimenez, Julio Ledezma, Silverio A. Lopez, Karen S. Martirosyan, Sue Anne Chew

Health & Biomedical Sciences Faculty Publications

The survival of patients with glioblastoma multiforme (GBM), the most common and invasive form of malignant brain tumors, remains poor despite advances in current treatment methods including surgery, radiotherapy, and chemotherapy. Minocycline is a semi-synthetic tetracycline derivative that has been widely used as an antibiotic and more recently, it has been utilized as an antiangiogenic factor to inhibit tumorigenesis. The objective of this study was to investigate the utilization of electrospraying process to fabricate minocycline-loaded poly(lactic-co-glycolic acid) (PLGA) microparticles with high drug loading and loading efficiency and to evaluate their ability to induce cell toxicity in human glioblastoma (i.e., U87-MG) …

The Effect Of A Mn(Iii)Tetrakis(4-Benzoic Acid)Porphyrin (Mntbap) Coating On He Chronic Recording Performance Of Planar Silicon Intracortical Microelectrode Arrays, George F. Hoeferlin, Lindsey N. Druschel, Olivia K. Krebs, Dhariyat M. Menendez, Jonathan L. Duncan, Jichu Zhang, Gaurav Mittal, Jeffrey R. Capadona

Faculty Scholarship

Intracortical microelectrode arrays (MEAs) are used to record neural activity. However, their implantation initiates a neuroinflammatory cascade, involving the accumulation of reactive oxygen species, leading to interface failure. Here, we coated commercially-available MEAs with Mn(III)tetrakis(4-benzoic acid)porphyrin (MnTBAP), to mitigate oxidative stress. First, we assessed the in vitro cytotoxicity of modified sample substrates. Then, we implanted 36 rats with uncoated, MnTBAP-coated (“Coated”), or (3-Aminopropyl)triethoxysilane (APTES)-coated devices – an intermediate step in the coating process. We assessed electrode performance during the acute (1–5 weeks), sub-chronic (6–11 weeks), and chronic (12–16 weeks) phases after implantation. Three subsets of animals were euthanized at different …

Self-Assembling Peptide-Based High-Relaxivity Prostate-Cancer-Targeted Mri Contrast Agents, Andrew O'Brien

Theses

Magnetic Resonance Imaging (MRI) is a widely used, highly effective imaging method for diagnosing cancer and guiding treatment. MRI contrast agents enhance image quality, with higher relaxivity (r1) contrast agents providing the best utility. Attaching a cancer-specific-targeting group enhances the image further. Therefore, a high-relaxivity cancer-targeted contrast agent is a highly desirable tool for diagnosis and treatment. Our group previously synthesized a peptide-based MRI contrast agent with the Ala(Gd-DO3A) chelator that targeted the prostate cancer biomarker PSMA using the small-molecule ligand DCL. While this agent displayed nominal relaxivity, it was serendipitously found that the relaxivity of a synthetic intermediate containing …

The Pseudomonas Syringae Pv. Tomato Dc3000 Effector Hopd1 Interferes With Cellular Dynamics Associated With The Function Of The Plant Immune Protein Atnhr2b, Luis Francisco Marin-Ponce, Catalina Rodriguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas

Entomology and Plant Pathology Faculty Publications and Presentations

The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes disease in tomato, in the model plant Arabidopsis thaliana, and conditionally in Nicotiana benthamiana. The pathogenicity of Pst DC3000 is mostly due to bacterial virulence proteins, known as effectors, that are translocated into the plant cytoplasm through the type III secretion system (T3SS). Bacterial type III secreted effectors (T3SEs) target plants physiological processes and suppress defense responses to enable and support bacterial proliferation. The Pst DC3000 T3SE HopD1 interferes with plant defense responses by targeting the transcription factor NTL9. This work shows that HopD1 also targets the …

Microfluidic Coaxial 3d Bioprinting Of Cell-Laden Microfibers And Microtubes For Salivary Gland Tissue Engineering, Yu Yin, Ephraim J Vázquez-Rosado, Danielle Wu, Vignesh Viswananthan, Andrew Farach, Mary C Farach-Carson, Daniel A Harrington

Faculty, Staff and Student Publications

Replacement therapy for the salivary gland (SG) remains an unmet clinical need. Xerostomia ("dry mouth") due to hyposalivation can result from injury or disease to the SG, such as salivary acinar death caused by radiation therapy (RT) for head and neck squamous cell carcinoma (HNSCC). Currently, only palliative treatments exist for xerostomia, and many patients endure deteriorated oral health and poor quality of life. Tissue engineering could offer a permanent solution for SG replacement by isolating healthy SG tissues prior to RT, expanding its cells in vitro, and recreating a functional salivary neogland for implantation post-RT. 3D bioprinting methods potentiate …

Ensuring Trust In Genomics Research, Erman Ayday, Jaideep Vaidya, Xiaoqian Jiang, Amalio Telenti

Faculty, Staff and Student Publications

Reproducibility, transparency, representation, and privacy underpin the trust on genomics research in general and genome-wide association studies (GWAS) in particular. Concerns about these issues can be mitigated by technologies that address privacy protection, quality control, and verifiability of GWAS. However, many of the existing technological solutions have been developed in isolation and may address one aspect of reproducibility, transparency, representation, and privacy of GWAS while unknowingly impacting other aspects. As a consequence, the current patchwork of technological tools only partially and in an overlapping manner address issues with GWAS, sometimes even creating more problems. This paper addresses the progress in …

Exercise Training Reduces The Inflammatory Response And Promotes Intestinal Mucosa-Associated Immunity In Lynch Syndrome, Nan Deng, Laura Reyes-Uribe, Johannes F Fahrmann, Whittney S Thoman, Mark F Munsell, Jennifer B Dennison, Eunice Murage, Ranran Wu, Ernest T Hawk, Selvi Thirumurthi, Patrick M Lynch, Christina M Dieli-Conwright, Alexander J Lazar, Sonali Jindal, Khoi Chu, Manoj Chelvanambi, Karen Basen-Engquist, Yisheng Li, Jennifer A Wargo, Florencia Mcallister, James P Allison, Padmanee Sharma, Krishna M Sinha, Samir Hanash, Susan C Gilchrist, Eduardo Vilar

Faculty, Staff and Student Publications

PURPOSE: Lynch syndrome (LS) is a hereditary condition with a high lifetime risk of colorectal and endometrial cancers. Exercise is a non-pharmacologic intervention to reduce cancer risk, though its impact on patients with LS has not been prospectively studied. Here, we evaluated the impact of a 12-month aerobic exercise cycling intervention in the biology of the immune system in LS carriers.

PATIENTS AND METHODS: To address this, we enrolled 21 patients with LS onto a non-randomized, sequential intervention assignation, clinical trial to assess the effect of a 12-month exercise program that included cycling classes 3 times weekly for 45 minutes …

Nanobomb Optical Coherence Elastography In Multilayered Phantoms, Maryam Hatami, Dmitry Nevozhay, Manmohan Singh, Alexander Schill, Paul Boerner, Salavat Aglyamov, Konstantin Sokolov, Kirill V Larin

Faculty, Staff and Student Publications

Many tissues are composed of layered structures, and a better understanding of the changes in the layered tissue biomechanics can enable advanced guidance and monitoring of therapy. The advent of elastography using longitudinally propagating shear waves (LSWs) has created the prospect of a high-resolution assessment of depth-dependent tissue elasticity. Laser activation of liquid-to-gas phase transition of dye-loaded perfluorocarbon (PFC) nanodroplets (a.k.a., nanobombs) can produce highly localized LSWs. This study aims to leverage the potential of photoactivation of nanobombs to incudce LSWs with very high-frequency content in wave-based optical coherence elastography (OCE) to estimate the elasticity gradient with high resolution. In …

A Framework For Standardised Tissue Sampling And Processing During Resection Of Diffuse Intracranial Glioma: Joint Recommendations From Four Rano Groups, Philipp Karschnia, Marion Smits, Guido Reifenberger, Emilie Le Rhun, Benjamin M Ellingson, Norbert Galldiks, Michelle M Kim, Jason T Huse, Oliver Schnell, Patrick N Harter, Malte Mohme, Expert Rater Panel, Louisa Von Baumgarten, Nathalie L Albert, Raymond Y Huang, Minesh P Mehta, Martin Van Den Bent, Michael Weller, Michael A Vogelbaum, Susan M Chang, Mitchel S Berger, Joerg-Christian Tonn

Faculty, Staff and Student Publications

Surgical resection represents the standard of care for people with newly diagnosed diffuse gliomas, and the neuropathological and molecular profile of the resected tissue guides clinical management and forms the basis for research. The Response Assessment in Neuro-Oncology (RANO) consortium is an international, multidisciplinary effort that aims to standardise research practice in neuro-oncology. These recommendations represent a multidisciplinary consensus from the four RANO groups: RANO resect, RANO recurrent glioblastoma, RANO radiotherapy, and RANO/PET for a standardised workflow to achieve a representative tumour evaluation in a disease characterised by intratumoural heterogeneity, including recommendations on which tumour regions should be surgically sampled, …

Air-Side Fouling Of Finned Heat Exchangers: Part 1, Review And Proposed Test Protocol, H. V. Inamdar, E. A. Grol, Justin A. Weibel, S. V. Garimella

CTRC Research Publications

No abstract provided.

The Impacts Of Embryo Development And Asynchronous Hatching On Morphology, Growth, And Development Of Larval Burbot (Lota Lota)., Andrew J. Shapiro

All NMU Master's Theses

Burbot (Lota lota) are native fish in Lake Superior and typically spawn during the winter under the ice or by migrating up rivers. The early life history of larval burbot, where they dispurse, how fast they develop, and what their survival rates have not been extensively studied. Asynchronous hatching is a strategy used by other cod species as a bet-hedging strategy to ensure that some larvae are hatched in more advantageous conditions in a varying environment. Asynchronous hatching has been documented in burbot, but the extent of the period and the impacts that asynchrony has on the development of …

Gene Variants In Bola-Dmb, Decr1, Fasn And Srebf1 Associated With Conceptus Death On Day 16 Of Pregnancy In Holstein Cows [Abstract], Carolina L. Gonzalez-Berrios, Courtney Bowden, Pablo J. Pinedo, Jeanette V. Bishop, Hana Van Campen, Thomas R. R. Hansen, Milton G. Thomas

United States Department of Agriculture Wildlife Services: Staff Publications

Investigating the influences of single nucleotide polymorphisms (SNP) associated with conceptus death (CD) on day 16 of pregnancy could distinguish cows who have inferior value for fertility traits. We hypothesized that CD is associated with SNP genotypes that disrupt maternal recognition and lead to early pregnancy loss. RNA sequences from Holstein cow pregnancies (n = 15) with normal conceptuses (Norm) or CD were subjected to SNP discovery via Qiagen CLC Genomics Workbench. Selected SNP were based on previous fertility studies, differentially expressed genes within pregnancy development analyses and proximity to SNP associated with fertility traits in the Cattle QTL …

An Economic Evaluation Of The Use Of Non-Combusted Alternatives Using A Cost Of Illness Approach: The Philippine Case, Christopher James R. Cabuay

Angelo King Institute for Economic and Business Studies (AKI)

Smoking continues to be one of the leading causes of death and disability around the world. Recent health studies, however, have reported that these diseases are more likely to be due to the smoke from burning rather than the actual nicotine content. This study uses a cost of illness approach in estimating the cost of smoking-related illness in the Philippines and calculating the potential reduction in costs if a significant portion of the adult smoking population switches to the exclusive use of non-combusted alternatives (NCAs), which drastically reduces the risk of contracting smoking-related diseases. This study finds that cost reductions …

Ionic Partitioning Of Kcl In Aot Reverse Micelles From Molecular Dynamics Simulations, Max Crowder, Isabel Lizarraga, Joshua D. Patterson, Arun K. Sharma

Biology, Agriculture and Chemistry Faculty Publications and Presentations

Reverse micelles are an important class of nanoreactors providing an array of applications. The structure and dynamics of aqueous reverse micelles have been intensely investigated, yet there are many subtleties involved in characterizing the arrangement of water and ionic species within these aggregates. The interfacial arrangement of water and dopants added to bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) reverse micelles were investigated by fully atomistic molecular dynamics simulations. Reverse micelles of increasing water to surfactant ratio were doped with concentrations of KCl from 0.0 to 1.0 M to analyze the partitioning of ions and water. Detailed analysis of pair density functions …

Blood Will Tell: Eugenics Education At A Twentieth-Century Southern University, Meg Langhorne, Alison Bell

Southern Anthropologist

During the 1920s and ‘30s, Washington and Lee University (W&L) biology students visited local families suspected of “degeneracy.” At the direction of their professor and with the support of social workers, physicians, and other authorities, students recorded generational histories as well as such variables as age, material conditions, educational level, employment, illnesses, and supposed proclivities toward promiscuity, alcoholism, illegitimacy, “idiocy,” and “feeblemindedness.” W&L Special Collections and Archives contains 25 of these eugenics term papers. Together they document ways that young White men – many from well-to-do southern families – learned or affirmed that their social position was a function of …