Digital Commons Network^™

Genome-Wide Mutagenesis To Investigate The N-Terminal Methylome: The Protective Effects Of Hsp31 And Other Methylated Proteins In Yeast, James Rooney, Jacob Lindsey

The Journal of Purdue Undergraduate Research

The purpose of this study was to understand the role of methylation in regulating the cellular stress response of Hsp31 in Saccharomyces cerevisiae yeast cells. Hsp31 is known to be methylated by the N-terminal methyltransferase Tae1. Changing the methylation site can affect the methylation status of Hsp31, which may play a role in the protective activity of Hsp31 against cellular stress. GLO1 is a gene in yeast involved in catalyzing the detoxification of methylglyoxal (MGO), which is a by-product of glycolysis. We established that S. cerevisiae in the glo1Δ and background is sensitive to cellular stress by MGO. Mutant strains …

Machine Learning Of Big Data: A Gaussian Regression Model To Predict The Spatiotemporal Distribution Of Ground Ozone, Jerry Gu

The Journal of Purdue Undergraduate Research

Tracking pollution levels on the ground is important to the environment and public health. One of the pollutants of concern is ozone, which, at high concentrations, can cause respiratory and cardiovascular problems. The National Center for Atmospheric Research (NCAR) has published valuable ozone data obtained from ground-based sensors installed at selected locations. Because it is unfeasible to measure the exact ozone levels everywhere at any time, it would be valuable to predict the temporal-spatial distributions of ozone concentration based on existing data. This would help us better understand the patterns and trends in the data and make better decisions to …

A Computational Profile Of Invasive Lionfish In Belize: A New Insight On A Destructive Species, Joshua E. Balan

The Journal of Purdue Undergraduate Research

Since their discovery in the region in 2009, invasive Indonesian-native lionfish have been taking over the Belize Barrier Reef. As a result, populations of local species have dwindled as they are either eaten or outcompeted by the invaders. This has led to devastating losses ecologically and economically; massive industries in the local nations, such as fisheries and tourism, have suffered greatly. Attempting to combat this, local organizations, from nonprofits to ecotourism companies, have been manually spear-hunting them on scuba dives to cull the population. One such company, Reef Conservation Institute (ReefCI), operating out of Tom Owens Caye outside of Placencia, …

Parentally Exposed Zebrafish Larvae Have Altered Craniofacial Measurements: Multigeneration Developmental Atrazine Toxicity, Isabelle Akoro

The Journal of Purdue Undergraduate Research

Atrazine is a herbicide used throughout the midwestern United States to prevent broadleaf weeds in crops. The U.S. Environmental Protection Agency (EPA) has set the maximum contaminant level at 3 ppb (μg/L) in drinking water. Atrazine is an endocrine disrupter interfering with the function of hormones and disrupting normal physiology and homeostasis throughout development and the life course of an organism. The zebrafish model was used to test the hypothesis that an embryonic parental atrazine exposure will cause modifications in morphology in developing offspring. AB adult zebrafish were bred. Embryos were collected and exposed to atrazine concentrations of 0, 0.3, …

Around And About Illinois Masonic Medical Center, 1970, V5 N2, Summer, Advocate Aurora Health

Historical Documents - Combined

Illinois Masonic Hospital, Chicago, IL: Issue of "Around & About Illinois Masonic Medical Center", a quarterly community newsletter. This issue has updates and news about staff, fundraising, events, and research. Also included is a directory of new interns and residents.

There is a follow up to the story about the "Tranquility Ball" and Buzz Aldrin's boot print from the Winter 1969-70 issue. The bronzed boot print was exhibited around Chicago and Tiffany & Co. created commemorative jewelry featuring the boot print that was sold as a fundraising effort.

Around And About Illinois Masonic Medical Center, 1971, V6 N2, Summer/Fall, Advocate Aurora Health

Historical Documents - Combined

Illinois Masonic Hospital, Chicago, IL: Issue of "Around & About Illinois Masonic Medical Center", a quarterly community newsletter. This issue has updates and news about staff, fundraising, events, and research. Also included is a roster of residents and interns.

Due Process And Equal Protection: A Constitutional Approach To Same-Sex Marriage, Ashley Musselman

Tennessee Journal of Law and Policy

No abstract provided.

Just What The Doctor Ordered: The Need For Cross-Cultural Education In Law Schools, Andrew King-Ries

Tennessee Journal of Law and Policy

No abstract provided.

Environmental Impact Assessment In Post-Colonial Societies: Reflections On The Proposed Expansion Of The Panama Canal, Carmen G. Gonzalez

Tennessee Journal of Law and Policy

No abstract provided.

Saving Biodiversity At The Crossroads Of The Americas, Colin Crawford

Tennessee Journal of Law and Policy

No abstract provided.

A Philosophy Primer, Anthony Cunningham

Philosophy Faculty Publications

This philosophy primer serves as an introduction to the general pursuit of philosophy and the practice of sound reasoning. The primer identifies and explains seven important guiding ideals for conducting any intellectual inquiry, along with highlighting eight “good moves” to cultivate and eighteen “bad moves” to avoid.

Innovative Microwave-Assisted Biosynthesis Of Copper Oxide Nanoparticles Loaded With Platinum(Ii) Based Complex For Halting Colon Cancer: Cellular, Molecular, And Computational Investigations, Nada K. Sedky, Iten M. Fawzy, Afnan Hassan, Noha Khalil Mahdy, Samir N. Shamma, Reem T. Attia, Mohammad Y. Alfaifi, Serag Eldin Elbehairi, Fatma A. Mokhtar, Sherif Ashraf Fahmy

Faculty Journal Articles

In the current study, we biosynthesized copper oxide NPs (CuO NPs) utilizing the essential oils extracted from Boswellia carterii oleogum resin, which served as a bioreductant and capping agent with the help of microwave energy. Afterwards, the platinum(ii) based anticancer drug, carboplatin (Cr), was loaded onto the CuO NPs, exploiting the electrostatic interactions forming Cr@CuO NPs. The produced biogenic NPs were then characterized using zeta potential (ZP), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction spectroscopy (XRD), and Fourier transform infrared spectroscopy (FTIR) techniques. In addition, the entrapment efficiency and release profile of the loaded Cr were evaluated. Thereafter, SRB assay …

The Rider (2024-01-29), Natalie Lapsley

The Rider Archives

Vol.9 No.17

Patient-Derived Models Of Cancer In The Nci Pdmc Consortium: Selection, Pitfalls, And Practical Recommendations., Amber N Habowski, Deepthi P Budagavi, Sandra D Scherer, Arin B Aurora, Giuseppina Caligiuri, William F Flynn, Ellen M Langer, Jonathan R Brody, Rosalie C Sears, Giorgia Foggetti, Anna Arnal Estape, Don X Nguyen, Katerina A Politi, Xiling Shen, David S Hsu, Donna M Peehl, John Kurhanewicz, Renuka Sriram, Milagros Suarez, Sophie Xiao, Yuchen Du, Xiao-Nan Li, Nora M Navone, Estefania Labanca, Christopher D Willey

Faculty Research 2024

For over a century, early researchers sought to study biological organisms in a laboratory setting, leading to the generation of both in vitro and in vivo model systems. Patient-derived models of cancer (PDMCs) have more recently come to the forefront of preclinical cancer models and are even finding their way into clinical practice as part of functional precision medicine programs. The PDMC Consortium, supported by the Division of Cancer Biology in the National Cancer Institute of the National Institutes of Health, seeks to understand the biological principles that govern the various PDMC behaviors, particularly in response to perturbagens, such as …

Miniaturized Fluorescence Ph Sensor With Assembly Free Ball Lens On A Tapered Multimode Optical Fiber, Bohong Zhang, Farhan Mumtaz, Muhammad Roman, Dinesh Reddy Alla, Rex E. Gerald, Jie Huang

Electrical and Computer Engineering Faculty Research & Creative Works

In biochemistry, the absence of a compact, assembly-free pH sensor with high sensitivity and signal-to-noise ratio has been a persistent hurdle in achieving accurate pH measurements in real time, particularly in complex liquid environments. This manuscript introduces what we believe to be a novel solution in the form of a miniaturized pH sensor utilizing an assembly-free ball lens on a tapered multimode optical fiber (TMMF), offering the potential to revolutionize pH sensing in biochemical applications. A multimode optical fiber (MMF) was subjected to tapering processes, leading to the creation of an ultra-thin needle-like structure with a cross-sectional diameter of about …

Aspen Recovery In Northern Yellowstone: A Comment On Brice Et Al. (2021), Luke E. Painter, Robert L. Beschta, William J. Ripple

Aspen Bibliography

Aspen sapling recruitment increased as browsing by elk decreased, following the 1995–96 reintroduction of wolves in Yellowstone National Park. We address claims by Brice et al. (2021) that previous studies exaggerated recent aspen recovery. We conclude that their results actually supported previous work showing a trophic cascade benefiting aspen.

Robots As Pirates, Henry H. Perritt Jr.

Catholic University Law Review

Generative AI has created much excitement over its potential to create new works of authorship in the literary and graphical realms. Its underling machine-learning technology works by analyzing the relations among elements of preexisting material in enormous databases assembled from publicly available and licensed sources. Its algorithms “learn” to predict “what comes next” in different types of expression. A complete system thus can become glib in creating new factual summaries, essays, fictional stories and images.

A number of authors of the raw material used by Generative AI engines claim that the machine learning process infringes their copyrights. Careful evaluation of …

The Role Of The Cdk8 Kinase Module In Maintaining Proteostasis, Stephen Willis

Theses and Dissertations

The underlying etiology of numerous disease states results from perturbations in the maintenance of cellular proteostasis. Carcinogenesis relies on these perturbations to foster uncontrolled cell growth and eventual metastases, while neurodegenerative diseases are a consequence of such perturbations. Control of these processes occurs at numerous molecular levels, commonly starting with transcription. A key transcriptional complex that is involved is the CDK8 Kinase Module (CKM). The CKM is conserved from yeast to man, forming a tetrameric complex consisting of MED12, MED13, CDK8, and CCNC. The CKM has not only been implicated in a variety of cancers but also in a spectrum …

Mosaic Loss Of Y Chromosome Is Associated With Aging And Epithelial Injury In Chronic Kidney Disease, Parker C Wilson, Amit Verma, Yasuhiro Yoshimura, Yoshiharu Muto, Haikuo Li, Nicole P Malvin, Eryn E Dixon, Benjamin D Humphreys

2020-Current year OA Pubs

BACKGROUND: Mosaic loss of Y chromosome (LOY) is the most common chromosomal alteration in aging men. Here, we use single-cell RNA and ATAC sequencing to show that LOY is present in the kidney and increases with age and chronic kidney disease.

RESULTS: The likelihood of a cell having LOY varies depending on its location in the nephron. Cortical epithelial cell types have a greater proportion of LOY than medullary or glomerular cell types, which may reflect their proliferative history. Proximal tubule cells are the most abundant cell type in the cortex and are susceptible to hypoxic injury. A subset of …

Analysis Of Anemia And Iron Supplementation Among Glioblastoma Patients Reveals Sex-Biased Association Between Anemia And Survival, Ganesh Shenoy, Becky Slagle-Webb, Chachrit Khunsriraksakul, Bhavyata Pandya Shesh, Jingqin Luo, Vladimir Khristov, Nataliya Smith, Alireza Mansouri, Brad E Zacharia, Sheldon Holder, Justin D Lathia, Jill S Barnholtz-Sloan, James R Connor

2020-Current year OA Pubs

The association between anemia and outcomes in glioblastoma patients is unclear. We analyzed data from 1346 histologically confirmed adult glioblastoma patients in the TriNetX Research Network. Median hemoglobin and hematocrit levels were quantified for 6 months following diagnosis and used to classify patients as anemic or non-anemic. Associations of anemia and iron supplementation of anemic patients with median overall survival (median-OS) were then studied. Among 1346 glioblastoma patients, 35.9% of male and 40.5% of female patients were classified as anemic using hemoglobin-based WHO guidelines. Among males, anemia was associated with reduced median-OS compared to matched non-anemic males using hemoglobin (HR …

Spectrum Of The Lakes: Unveiling Water Color Of Minnesota's Sentinel Lakes With Satellite Remote Sensing For Water Quality Monitoring., Andrew J. Dooley

Theses and Dissertations

Surface waters are precious natural resources requiring costly time and labor for effective water quality monitoring. New applications of water color analysis by satellite remote sensing are a promising approach to water quality monitoring for scientific, industrial, recreational, and cultural benefit. Water color is an inherent proxy of ecological health and water quality. This research expands previous applications of lake water color analysis and pioneers water color chromaticity analysis for midcontinent lakes in Minnesota, USA. The results of this project are the first accounts of Minnesota’s Sentinel Lake water color, variability of water color by ecoregion, and temporal consistency of …

High Crystalline Quality Homoepitaxial Si-Doped Β-Ga2O3(010) Layers With Reduced Structural Anisotropy Grown By Hot-Wall Mocvd, D. Gogova, D. Q. Tran, V. Jokubavicius, L. Vines, M. Schubert, R. Yakimova, P. P. Paskov, V. Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

A new growth approach, based on the hot-wall metalorganic chemical vapor deposition concept, is developed for high-quality homoepitaxial growth of Si-doped single-crystalline β-Ga₂O₃ layers on (010)-oriented native substrates. Substrate annealing in argon atmosphere for 1 min at temperatures below 600 ^oC is proposed for the formation of epi-ready surfaces as a cost-effective alternative to the traditionally employed annealing process in oxygen-containing atmosphere with a time duration of 1 h at about 1000 ^oC. It is shown that the on-axis rocking curve widths exhibit anisotropic dependence on the azimuth angle with minima for in-plane direction …

Intouch Week Of January 29, 2024, New York Medical College

InTouch

• NYMC Faculty Mentor Two Semi-Finalists in Regeneron Science Talent Search Contest
• 2024 Brings New Year's Resolutions for NYMC Students
• LCME Element of the Week
• NYMC and TU to Host “Ninety Minutes: The Medical Magazine of the Web”
• Student Spotlight: Dextran Increases Risks After Carotid Endarterectomy

Health Disparities Among African American Multiple Myeloma Patients In The United States, Mechelle Antionette Rouse

Walden Dissertations and Doctoral Studies

No abstract provided.

The Effect Of Yoga On Affect And Optimism In Adolescents, Philip Allessi

Walden Dissertations and Doctoral Studies

No abstract provided.

The Role Of Family Support In The Self-Rated Health Of Older Adults In Eastern Nepal: Findings From A Cross-Sectional Study, Ivan Fumagalli, Stefano Pagani, Christian Vergara, Luca Dede', Dilachew A Adebo, Maurizio Del Greco, Antonio Frontera, Giovanni Battista Luciani, Gianluca Pontone, Roberto Scrofani, Alfio Quarteroni

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVE: Computational models of the cardiovascular system allow for a detailed and quantitative investigation of both physiological and pathological conditions, thanks to their ability to combine clinical-possibly patient-specific-data with physical knowledge of the processes underlying the heart function. These models have been increasingly employed in clinical practice to understand pathological mechanisms and their progression, design medical devices, support clinicians in improving therapies. Hinging upon a long-year experience in cardiovascular modeling, we have recently constructed a computational multi-physics and multi-scale integrated model of the heart for the investigation of its physiological function, the analysis of pathological conditions, and to …

Leveraging Trop2 Antibody-Drug Conjugates In Solid Tumors, Blessie Elizabeth Nelson, Funda Meric-Bernstam

Faculty, Staff and Student Publications

Antibody-drug conjugates (ADCs) have become the cornerstone of effective therapeutics in solid and hematological malignancies by harnessing potent cytotoxic payloads with targeted tumoricidal delivery. Since the monumental shift occurred with HER2-targeted ADCs, the discovery of the TROP2 antigen has revolutionized the landscape of ADC development. Moving beyond the traditional ADC design, multiple novel ADCs have successfully shaped and improved survival outcomes in patients with various tumor histologies. Here we review and contrast the clinical impact of the well-known TROP2 ADCs currently in clinical use. We also shed light on upcoming investigational TROP2 ADCs showing promise with novel ADC platforms.

Patient-Derived Models Of Cancer In The Nci Pdmc Consortium: Selection, Pitfalls, And Practical Recommendations, Amber N Habowski, Deepthi P Budagavi, Sandra D Scherer, Arin B Aurora, Giuseppina Caligiuri, William F Flynn, Ellen M Langer, Jonathan R Brody, Rosalie C Sears, Giorgia Foggetti, Anna Arnal Estape, Don X Nguyen, Katerina A Politi, Xiling Shen, David S Hsu, Donna M Peehl, John Kurhanewicz, Renuka Sriram, Milagros Suarez, Sophie Xiao, Yuchen Du, Xiao-Nan Li, Nora M Navone, Estefania Labanca, Christopher D Willey

Faculty, Staff and Student Publications

For over a century, early researchers sought to study biological organisms in a laboratory setting, leading to the generation of both in vitro and in vivo model systems. Patient-derived models of cancer (PDMCs) have more recently come to the forefront of preclinical cancer models and are even finding their way into clinical practice as part of functional precision medicine programs. The PDMC Consortium, supported by the Division of Cancer Biology in the National Cancer Institute of the National Institutes of Health, seeks to understand the biological principles that govern the various PDMC behaviors, particularly in response to perturbagens, such as …

Ahp Db: A Reference Database Of Proteins In The Human Aqueous Humor, Tae Jin Lee, Arnav Goyal, Garrett Jones, Joshua Glass, Vishal Doshi, Kathryn Bollinger, Lane Ulrich, Saleh Ahmed, Sai Karthik Kodeboyina, Amy Estes, Marc Töteberg-Harms, Wenbo Zhi, Shruti Sharma, Ashok Sharma

Faculty, Staff and Student Publications

The aqueous humor (AH) is a low-viscosity biofluid that continuously circulates from the posterior chamber to the anterior chamber of the eye. Recent advances in high-resolution mass-spectrometry workflows have facilitated the study of proteomic content in small-volume biofluids like AH, highlighting the potential clinical implications of the AH proteome. Nevertheless, in-depth investigations into the role of AH proteins in ocular diseases have encountered challenges due to limited accessibility to these workflows, difficulties in large-scale AH sample collection and the absence of a reference AH proteomic database. In response to these obstacles, and to promote further research on the involvement of …

Social Workers’ Education In Identifying Victims Of Human Trafficking, Simmone Lafette Jones

Walden Dissertations and Doctoral Studies

No abstract provided.