Digital Commons Network^™

Reactive Amine Functionalized Microelectrode Arrays Provide Short-Term Benefit But Long-Term Detriment To In Vivo Recording Performance, Lindsey N. Druschel, Pierce E. Boucher, George F. Hoeferlin, Dhariyat M. Menendez, Jonathan L. Duncan, Jeffrey R. Capadona

Faculty Scholarship

Intracortical microelectrode arrays (MEAs) are used for recording neural signals. However, indwelling devices result in chronic neuroinflammation, which leads to decreased recording performance through degradation of the device and surrounding tissue. Coating the MEAs with bioactive molecules is being explored to mitigate neuroinflammation. Such approaches often require an intermediate functionalization step such as (3-aminopropyl)triethoxysilane (APTES), which serves as a linker. However, the standalone effect of this intermediate step has not been previously characterized. Here, we investigated the effect of coating MEAs with APTES by comparing APTES-coated to uncoated controls in vivo and ex vivo. First, we measured water contact angles …

Compression Characteristics And Damage Mechanism Of Rose Orange, Lu Siyu, Luo Qiang, Lai Yushu, Xia Qiming

Food and Machinery

Objective: Reduced the damage of rose orange in the process of packaging and transportation, and provided a theoretical basis for postharvest storage and spectral quality testing. Methods: According to the basic geometric data of rose orange and the maximum compression force and puncture force obtained from TPA test and puncture test, the finite element model was constructed to simulate the compression of rose orange. Results: The diameter of rose orange ranged from 58.2 mm to 78.6 mm, the average weight was 169.746 g, the average thickness of peel at the equator was 3.1 mm, the maximum puncture force was 6.26 …

Preparation, Structural Characteristics And Antioxidant Activity Of Cardamine Violifolia Oligopeptides, Wen Xiaolong, Xiang Jiqian, Zhang Liang, Li Yajie, Long Lan

Food and Machinery

Objective: This study aimed to prepare oligopeptidess of Camelina violifolia and expand its application range. Methods: C. violifolia protein was hydrolyzed by alkaline protease, and then used ultrafiltration membrane for isolating and concentrating, and obtained oligopeptides by freeze-drying. The structure and antioxidant activity of C. violifolia oligopeptides were analyzed. Results: The optimum conditions for the hydrolysis of C.violifolia protein were as follows: hydrolysis temperature 50 ℃, pH 10, enzyme dosage 4%, substrate concentration 2%, and hydrolysis degree 17.9%. The C. violifolia oligopeptides contents of protein and polypeptide were 49.01% and 42.87%, respectively. The oligopeptides had a strong absorption band at …

Review Of Caenorhabditis Elegans In Food Nutritional Function Evaluation, Chen Wang, He Hailun, Zheng Yanjun, Yi Cuiping

Food and Machinery

As an ideal model organism, Caenorhabditis elegans has been increasingly used in the evaluation of the nutritional function of food. Compared with other models such as cells and mice, C. elegans have the advantages of small size, short lifespan, high genetic homology with humans, easy observation of research results, and large batch culture in the laboratory. Therefore, the article summarizes the research methods, potential mechanisms and research progress of C. elegans in food nutritional function such as anti-obesity, antioxidant and anti-aging. In order to provide a reference for new methods in the evaluation of food nutrition function.

Research Progress On Polyphenols In Chestnut Rose Fruit, Yin Yanyan, Zhang Jian, Lu Lunwei, Li Lulu, Zhang Deqin

Food and Machinery

Chestnut rose fruit, known as "Three King fruits", has high edible value and medicinal value. Polyphenols are the second major functional component of chestnut rose fruit with pharmacological and health functions such as antioxidant, regulation of glucose and lipid metabolism, anti-inflammatory and bacteriostatic, antialcoholism and liver protection, and have great potential for food development. This review summarized the research progress of the classification, extraction, qualitative and quantitative analysis, and bioactivity of chestnut rose fruit polyphenols in recent years and prospected the future development direction.

Selective Neural Inhibition Via Photobiomodulation Alleviates Behavioral Hypersensitivity Associated With Small Sensory Fiber Activation, Andrew Buzza, Kalista Tapas, Junqi Zhuo, Stephen J. Lewis, Michael W. Jenkins, Michael Moffitt

Faculty Scholarship

Objective: Photobiomodulation at higher irradiances has great potential as a pain-alleviating method that selectively inhibits small diameter nerve fibers and corresponding sensory experiences, such as nociception and heat sensation. The longevity and magnitude of these effects as a function of laser irradiation parameters at the nerve was explored. Methods: In a rodent chronic pain model (spared nerve injury—SNI), light was applied directly at the sural nerve with four delivery schemes: two irradiance levels (7.64 and 2.55 W/cm2) for two durations each, corresponding to either 4.8 or 14.4 J total energy, and the effect on sensory hypersensitivities was evaluated. Results: At …

Asosiasi Jaminan Sosial Dengan Partisipasi Kerja Penduduk Lansia Di Indonesia, Evelyn Fairuz Wibowo, Dwini Handayani

Jurnal Ekonomi Kependudukan dan Keluarga

Indonesia has entered a period of aging population. The number of elderly people has increased sharply without social security ownership, making the elderly population vulnerable to poverty. Financial demands make the elderly must continue to work in their old age, even though their biological and psychological conditions are declining. The study aims to analyzes the effect of social insurance (pension insurance and health insurance) on the work participation of the elderly population which is controlled based on individual, household, and social characteristics. Using secondary data from the 2020 National Socioeconomic Survey and analyzed using logistic biner regression, this …

On Modeling Arterial Blood Flow With Or Without Solute Transport And In Presence Of Atherosclerosis, Daniel N. Riahi, Saulo Orizaga

School of Mathematical & Statistical Sciences Faculty Publications

In this article, we review previous studies of modeling problems for blood flow with or without transport of a solute in a section of arterial blood flow and in the presence of atherosclerosis. Moreover, we review problems of bio-fluid dynamics within the field of biophysics. In most modeling cases, the presence of red blood cells in the plasma is taken into account either by using a two-phase flow approach, where blood plasma is considered as one phase and red blood cells are counted as another phase, or by using a variable viscosity formula that accounts for the amount of hematocrit …

Association Between Regulatory T Cells And Ischemic Heart Disease: A Mendelian Randomization Study, Yucheng Hou, Ke Si, Jingyue Yang, Tan Liu, Basel Abdelazeem, Nonthikorn Theerasuwipakorn, Jingwei Zhao, Zhenya Shen

Faculty, Staff and Student Publications

BACKGROUND: An imbalance of innate and acquired immune responses is significantly involved in the pathophysiology of coronary atherosclerosis and the occurrence of ischemic heart disease (IHD). Regulatory T cells (Tregs) play an essential regulatory role in atherosclerotic plaque formation and maintenance; therefore, dysfunction of Tregs triggers the formation of atherosclerotic plaques and accelerates their progression. However, due to the inherent limitations of observational research, clinical evidence is limited concerning the relationship between the variation in peripheral Tregs and the risk of IHD, and the cause-and-effect relationship between these factors is unclear. Mendelian randomization (MR) uses genetic variation as a proxy …

Awareness And Attitude Regarding Menstrual Cup Usage Among Nursing Students In Selected Areas Of Udupi District., Royden Gomes, Monica Dsouza, Neena Quadras, Wendy H. Sellate, Maria Pais, Ranjani P

Manipal Journal of Nursing and Health Sciences

Introduction

Menstruation is a complex, coordinated biological process that signals the start of a woman's reproductive life. Women and adolescent girls all across the world require menstrual hygiene management solutions that are safe, effective, and affordable. The market has a wide variety of alternatives, including sanitary napkins, tampons, menstrual cups, and more. Menstrual cups are one of the world's safest, most comfortable, environmentally friendly, and sustainable menstrual hygiene management options.

Methods

The current study used a descriptive survey methodology with a purposive sample to determine the awareness and attitude toward menstrual cup use among 60 nursing students. Structured questionnaires were …

Comprehensive Characterization Of Polyproline Tri-Helix Macrocyclic Nanoscaffolds For Predictive Ligand Positioning, Chia-Lung Tsai, Je-Wei Chang, Kum-Yi Cheng, Yu-Jing Lan, Yi-Cheng Hsu, Qun-Da Lin, Tzu-Yuan Chen, Orion Shih, Chih-Hsun Lin, Po-Hsun Chiang, Mantas Simenas, Vidmantas Kalendra, Yun-Wei Chiang, Chun-Hsien Chen, U-Ser Jeng, Sheng-Kai Wang

Faculty, Staff and Student Publications

Multivalent ligands hold promise for enhancing avidity and selectivity to simultaneously target multimeric proteins, as well as potentially modulating receptor signaling in pharmaceutical applications. Essential for these manipulations are nanosized scaffolds that precisely control ligand display patterns, which can be achieved by using polyproline oligo-helix macrocyclic nanoscaffolds via selective binding to protein oligomers and cell surface receptors. This work focuses on synthesis and structural characterization of different-sized polyproline tri-helix macrocyclic (PP3M) scaffolds. Through combined analysis of circular dichroism (CD), small- and wide-angle X-ray scattering (SWAXS), electron spin resonance (ESR) spectroscopy, and molecular modeling, a non-coplanar tri-helix loop structure with partially …

The Effectiveness Of Learning Videos To Improve Elementary School Teachers' Understanding Of The Learning Characteristics Of Students With Special Needs, Ade Kartini, Imas Diana Aprilia, Endang Rochyadi

Journal of ICSAR

The lack of understanding of elementary school teachers about the learning characteristics of students with special needs and the lack of adaptive learning media is allegedly factors that do not optimize learning. This study aims to measure the effectiveness of learning videos in improving elementary school teachers' understanding of the learning characteristics of students with special needs. The research method used is descriptive quantitative with One Group Pre-test and Post-test design. Data collection was carried out through tests with research subjects, namely six teachers from two elementary schools in Sumedang Regency. The results of this study indicate an increase in …

Impaired Neutrophil Extracellular Trap-Forming Capacity Contributes To Susceptibility To Chronic Vaginitis In A Mouse Model Of Vulvovaginal Candidiasis, Junko Yano, Paul L. Fidel

School of Dentistry Faculty Publications

Vulvovaginal candidiasis (VVC), caused by is characterized by aberrant inflammation by polymorphonuclear neutrophils (PMNs) in the vaginal lumen. Data from the established murine model shows that despite potent antifungal properties, PMNs fail to clear due to local heparan sulfate that inhibits the interaction between PMNs and , resulting in chronic vaginal immunopathology. To understand the role of neutrophil extracellular traps (NETs) in defense against at the vaginal mucosa, we investigated the NET-forming capacity of PMNs in chronic VVC-susceptible (CVVC-S/C3H) and -resistant (CVVC-R/CD-1) mouse strains. Immunofluorescence revealed the formation of NETs (release of DNA with PMN-derived antimicrobial proteins) in PMN cocultures …

Expression Of Atoh1, Gfi1, And Pou4f3 In The Mature Cochlea Reprograms Nonsensory Cells Into Hair Cells, Melissa M Mcgovern, Ishwar V Hosamani, Yichi Niu, Ken Y Nguyen, Chenghang Zong, Andrew K Groves

Faculty, Staff and Students Publications

Mechanosensory hair cells of the mature mammalian organ of Corti do not regenerate; consequently, loss of hair cells leads to permanent hearing loss. Although nonmammalian vertebrates can regenerate hair cells from neighboring supporting cells, many humans with severe hearing loss lack both hair cells and supporting cells, with the organ of Corti being replaced by a flat epithelium of nonsensory cells. To determine whether the mature cochlea can produce hair cells in vivo, we reprogrammed nonsensory cells adjacent to the organ of Corti with three hair cell transcription factors: Gfi1, Atoh1, and Pou4f3. We generated numerous hair cell–like …

Frozen Tissue Coring And Layered Histological Analysis Improves Cell Type-Specific Proteogenomic Characterization Of Pancreatic Adenocarcinoma, Sara R Savage, Yuefan Wang, Lijun Chen, Scott Jewell, Chelsea Newton, Yongchao Dou, Qing Kay Li, Oliver F Bathe, Ana I Robles, Gilbert S Omenn, Mathangi Thiagarajan, Hui Zhang, Galen Hostetter, Bing Zhang

Faculty, Staff and Students Publications

BACKGROUND: Omics characterization of pancreatic adenocarcinoma tissue is complicated by the highly heterogeneous and mixed populations of cells. We evaluate the feasibility and potential benefit of using a coring method to enrich specific regions from bulk tissue and then perform proteogenomic analyses.

METHODS: We used the Biopsy Trifecta Extraction (BioTExt) technique to isolate cores of epithelial-enriched and stroma-enriched tissue from pancreatic tumor and adjacent tissue blocks. Histology was assessed at multiple depths throughout each core. DNA sequencing, RNA sequencing, and proteomics were performed on the cored and bulk tissue samples. Supervised and unsupervised analyses were performed based on integrated molecular …

Clinical Practice Guideline-Inconsistent Management Of Fever And Neutropenia In Pediatric Oncology: A Children's Oncology Group Study, L. Lee Dupuis, Brian T. Fisher, A. J. Sugalski, Allison Caren Grimes, Michelle Nuño, S. Ramakrishnan, M. P. Beauchemin, Paula D. Robinson, Nancy Santesso, Alexandra Walsh, A. R. Wrightson, Lolie Yu, Susan K. Parsons, Lillian Sung

School of Medicine Faculty Publications

Background: The primary objective was to measure the proportion of episodes where care delivery was inconsistent with selected recommendations of a clinical practice guideline (CPG) on fever and neutropenia (FN) management. The influence of site size on CPG-inconsistent care delivery, and association between patient outcomes and CPG-inconsistent care were described. Methods: This retrospective, multicenter study included patients less than 21 years old with cancer who were at high risk of poor FN outcomes and were previously enrolled to a Children's Oncology Group (COG) study at participating National Cancer Institute Community Oncology Research Program (NCORP) institutions from January 2014 through December …

Back Matter

The Journal of Purdue Undergraduate Research

No abstract provided.

Cover Image Research Summary

The Journal of Purdue Undergraduate Research

Researchers in the Hans Lab examine the behavior and development of forensically relevant insects, particularly blow flies. Both graduate and undergraduate students participate in research on various topics related to decomposition and insect activity that assist with death investigations.

Alumni Spotlight: Caleb Hettinger

The Journal of Purdue Undergraduate Research

Caleb Hettinger published an article in volume 8 (2018) of the Journal of Purdue Undergraduate Research during his time spent completing his baccalaureate degree.

Toward Improved Global Food Security: Uncovering How Tomatoes Fight Root-Knot Nematodes, Chingyan H. Huang

The Journal of Purdue Undergraduate Research

No abstract provided.

Exploring The Evolution Of Callose Synthase In Green Plants, Giovanna Durante

The Journal of Purdue Undergraduate Research

No abstract provided.

Model Selection Through Cross-Validation For Supervised Learning Tasks With Manifold Data, Derek Brown

The Journal of Purdue Undergraduate Research

No abstract provided.

The Effects Of Wildfire Aerosol Emissions On Air Quality, Emma Braun, Audrey Shirley

The Journal of Purdue Undergraduate Research

No abstract provided.

Comparing Effects Of Atrazine Exposure On Neuroendocrine Molecular Targets At Two Developmental Exposure Periods In The Zebrafish, Jenna Swihart

The Journal of Purdue Undergraduate Research

No abstract provided.

Formulation Of Preservation Solutions For Model Generation With In Vivo Tissue Morphology, Holly Pickett

The Journal of Purdue Undergraduate Research

No abstract provided.

Active Herbicide Ingredients In Roundup Ready Xtend Products, Glyphosate And Dicamba, Results In Hypoactivity In Zebrafish Larvae When Exposed During Development, Ryker Bond

The Journal of Purdue Undergraduate Research

No abstract provided.

Lake Michigan Shoreline Landowner Survey, Colby Smock

The Journal of Purdue Undergraduate Research

No abstract provided.

Gossypium Hirsutum As A Study Species To Understand Plant Responses To Drought Stress, Sam Schafer

The Journal of Purdue Undergraduate Research

No abstract provided.

Laboratory Screening Of Sorghum Lines For Incompatibility: A Postattachment Resistance Mechanism To The Parasitic Weed Striga Hermonthica, Cameron Matthews

The Journal of Purdue Undergraduate Research

No abstract provided.

The Role Of Vocal Development Patterns: Predicting Neurogenetic Risk In Infancy Using Early Vocal Development And Sex, Alyssa Cregg, Rachel Siela, Olivia Battaglia, Kaylee Bobay, Madison Chin, Athena Fordwor, Conghao Gao, Deeksha Handa, Erin Lee, Tiernan Mcdivitt, Grace Strabala, Victoria Tuell, Laurel Williams

The Journal of Purdue Undergraduate Research

Extant literature documents a higher rate of language/speech disorders in males; however, despite sex being a potential moderator of outcomes, we do not know what role it plays in early vocal behavior of infants at high risk for such disorders. The purpose of this study was to ask: (1) Do high-risk infants demonstrate atypical vocal development patterns? (2) Is the quality and quantity of early babble distinct for male and female infants, and does this pattern vary across risk? To answer these questions, we examined the canonical babbling ratio (CBR; the ratio of canonical syllables, those with a consonant and …