Digital Commons Network^™

Bioengineered Skin For Diabetic Foot Ulcers: A Scoping Review, Nathaniel R. Primous, Peter Elvin, Kathleen V. Carter, Hagner L. Andrade, Javier La Fontaine, Naohiro Shibuya, Cláudia Cristina Biguetti

School of Podiatric Medicine Publications

Diabetic foot ulcers (DFUs) pose a significant threat to individuals with diabetes mellitus (DM), such as lower limb amputation and severe morbidity. Bioengineered skin substitutes (BSS) are alternatives to traditional interventions for treating DFUs, but their efficacy compared to standard wound care (SWC) or other treatment types, such as allografts, remains unknown. A scoping review of human studies was conducted to identify current approaches in the treatment of DFUs using BSS as compared with other treatment options. Systematic searches in PubMed, Cochrane Library, and Web of Science were conducted to identify comparative studies that enrolled 10 or more patients and …

Use Of Crispr Interference For Efficient And Rapid Gene Inactivation In Fusobacterium Nucleatum, Peng Zhou, Bibek G C, Flynn Stolte, Chenggang Wu

Faculty, Staff and Student Publications

Gene inactivation by creating in-frame deletion mutations in Fusobacterium nucleatum is time consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPR interference (CRISPRi) system. This system employs the nuclease-inactive Streptococcus pyogenes Cas9 protein (dCas9), specifically guided to the gene of interest by a constantly expressed single-guide RNA (sgRNA). Mechanistically, this dCas9-sgRNA complex serves as an insurmountable roadblock for RNA polymerase, thus repressing the target gene transcription. Leveraging this system, we first examined two non-essential genes, ftsX and radD, which are pivotal for fusobacterial cytokinesis and coaggregation. Upon adding the inducer, theophylline, …

'Science By Consensus' Impedes Scientific Creativity And Progress: A Simple Alternative To Funding Biomedical Research, Nejat Düzgüneş

Pacific Faculty Work

The very low success rates of grant applications to the National Institutes of Health (NIH) and the National Science Foundation (NSF) are highly detrimental to the progress of science and the careers of scientists. The peer review process that evaluates proposals has been claimed arbitrarily to be the best there is. This consensus system, however, has never been evaluated scientifically against an alternative. Here we delineate the 15 major problems with the peer review process. We challenge the Science Advisor to the President, and the leadership of NIH, NSF, the U.S. National Academy of Sciences and other funding agencies throughout …

Antibody Profiling And Predictive Modeling Discriminate Between Kaposi Sarcoma And Asymptomatic Kshv Infection, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood

School of Biological Sciences: Faculty Publications

Protein-level immunodominance patterns against Kaposi sarcoma-associated herpesvirus (KSHV), the aetiologic agent of Kaposi sarcoma (KS), have been revealed from serological probing of whole protein arrays, however, the epitopes that underlie these patterns have not been defined. We recently demonstrated the utility of phage display in high-resolution linear epitope mapping of the KSHV latency-associated nuclear antigen (LANA/ORF73). Here, a VirScan phage immunoprecipitation and sequencing approach, employing a library of 1,988 KSHV proteome-derived peptides, was used to quantify the breadth and magnitude of responses of 59 sub-Saharan African KS patients and 22 KSHV-infected asymptomatic individuals (ASY), and ultimately to support an application …

The Impact Of Iron Supplementation On The Preterm Neonatal Gut Microbiome: A Pilot Study, Matthew Van Ormer, Maranda Thompson, Melissa K. Thoene, Jean-Jack Riethoven, Sathish Kumar Natarajan, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Objective The gastrointestinal microbiome in preterm infants exhibits significant influence on optimal outcomes–with dysbiosis shown to substantially increase the risk of the life-threatening necrotizing enterocolitis. Iron is a vital nutrient especially during the perinatal window of rapid hemoglobin production, tissue growth, and foundational neurodevelopment. However, excess colonic iron exhibits potent oxidation capacity and alters the gut microbiome–potentially facilitating the proliferation of pathological bacterial strains. Breastfed preterm infants routinely receive iron supplementation starting 14 days after delivery and are highly vulnerable to morbidities associated with gastrointestinal dysbiosis. Therefore, we set out to determine if routine iron supplementation alters the preterm gut …

Antibody Profiling And Predictive Modeling Discriminate Between Kaposi Sarcoma And Asymptomatic Kshv Infection, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood

School of Medicine Faculty Publications

Protein-level immunodominance patterns against Kaposi sarcoma-associated herpesvirus (KSHV), the aetiologic agent of Kaposi sarcoma (KS), have been revealed from serological probing of whole protein arrays, however, the epitopes that underlie these patterns have not been defined. We recently demonstrated the utility of phage display in high-resolution linear epitope mapping of the KSHV latency-associated nuclear antigen (LANA/ORF73). Here, a VirScan phage immunoprecipitation and sequencing approach, employing a library of 1,988 KSHV proteome-derived peptides, was used to quantify the breadth and magnitude of responses of 59 sub-Saharan African KS patients and 22 KSHV-infected asymptomatic individuals (ASY), and ultimately to support an application …

How Physicians’ Perceptions Of Satisfaction And Morale Affect Retention, Ernest George Britton

Walden Dissertations and Doctoral Studies

Physician turnover affects care delivery and contributes to costs, emphasizing the need for retention to minimize unnecessary costs of hiring, training, and lost productivity among healthcare organizations. Healthcare leaders tend to be concerned about the negative affects that physician turnover has on patient care, profitability, and organizational viability. The U.S. healthcare system may benefit from reduced expenditures associated with retention. Grounded in Ellenbecker’s job retention model, the purpose of this qualitative multiple-case study was to explore strategies healthcare organizational leaders use to retain employed primary care and internal medicine physicians. The participants comprised six physician leaders from three healthcare organizations …

Quantifying Wildfire Drivers And Predictability In Boreal Peatlands Using A Two-Step Error-Correcting Machine Learning Framework In Tefire V1.0, Rongyun Tang, Mingzhou Jin, Daniel M. Ricciuto, Anping Chen, Yulong Zhang

Biological and Agricultural Engineering Faculty Publications and Presentations

Wildfires are becoming an increasing challenge to the sustainability of boreal peatland (BP) ecosystems and can alter the stability of boreal carbon storage. However, predicting the occurrence of rare and extreme BP fires proves to be challenging, and gaining a quantitative understanding of the factors, both natural and anthropogenic, inducing BP fires remains elusive. Here, we quantified the predictability of BP fires and their primary controlling factors from 1997 to 2015 using a two-step correcting machine learning (ML) framework that combines multiple ML classifiers, regression models, and an error-correcting technique. We found that (1) the adopted oversampling algorithm effectively addressed …

Collagen Type Xii Is Undetectable In Keratoconus Bowman’S Layer, Mohammed Rigi, Hyeck-Soo Son, Loren Moon, Mario Matthaei, Divya Srikumaran, Albert S Jun, Charles G Eberhart, Uri S Soiberman

Faculty, Staff and Student Publications

PURPOSE: Corneal biomechanical failure is the hallmark of keratoconus (KC); however, the cause of this failure remains elusive. Collagen type XII (

METHODS: TaqMan quantitative PCR was performed on 31 corneal epithelium samples of progressive KC and myopic control eyes. Tissue microarrays were constructed using full-thickness corneas from 61 KC cases during keratoplasty and 18 non-KC autopsy eyes and stained with an antibody specific to COL12A1. Additionally,

RESULTS: COL12A1 expression was reduced at transcript levels in KC epithelium compared to controls (ratio: 0.58, p<0.03). Immunohistochemical studies demonstrated that COL12A1 protein expression in BL was undetectable, with reduced expression in KC epithelium, basement membrane, and stroma.

CONCLUSIONS: The apparent absence of COL12A1 in KC BL, together with the functional importance that

Predicting Lemna Growth Based On Climate Change And Eutrophication In Temperate Freshwater Drainage Ditches, Jared Feller, Mark Taylor, Paul Henry Lunt

School of Geography, Earth and Environmental Sciences

The Somerset Levels and Moors, UK is an area of high conservation value, including nationally and internationally protected wetland sites. Duckweed (Lemna) mats in the Somerset Levels and Moors are increasingly common and are an indication of hyper-eutrophication. Duckweed mats can contribute to increased management burdens and decreases in biodiversity. Research suggests duckweed will thrive in temperate regions as the climate warms. This study investigated the impact of climate warming and eutrophication on duckweed growth in the Somerset Levels and Moors using a previously published model to simulate duckweed growth from the years of 2020–2080. Simulated local climate change projections …

Progestin-Associated Meningiomatosis With Unusual Schwannoma-Like Morphology, Katherine A Krause, Jared K Woods, Alexandra J Golby, Eudocia Q Lee, Shyam Tanguturi, Zachary Spigelman, Azra H Ligon, Umberto De Girolami, Matthew Torre

Duncan NRI Faculty and Staff Publications

No abstract provided.

Novel Design And Analysis For Rare Disease Drug Development, Shein-Chung Chow, Annpey Pong, Susan S. Chow

Student Publications

For rare disease drug development, the United States (US) Food and Drug Administration (FDA) has indicated that the same standards as those for drug products for common conditions will be applied. To assist the sponsors in rare disease drug development, the FDA has initiated several incentive programs to encourage the sponsors in rare disease drug development. In practice, these incentive programs may not help in achieving the study objectives due to the limited small patient population. To overcome this problem, some out-of-the-box innovative thinking and/or approaches, without jeopardizing the integrity, quality, and scientific validity of rare disease drug development, are …

Revascularization Strategies For Acute And Chronic Mesenteric Ischemia: A Narrative Review, Jacob J Gries, Takashi Sakamoto, Bing Chen, Hafeez Ul Hassan Virk, Mahboob Alam, Chayakrit Krittanawong

Faculty, Staff and Students Publications

Mesenteric ischemia is a challenging condition characterized by insufficient blood perfusion to the mesentery and, consequently, intestinal tissues that continues to perplex clinicians. Despite its low prevalence, the condition’s variable clinical presentation and elusive radiographic diagnosis can delay life-saving interventions in the acute setting and deteriorate the quality of life of patients when left undiagnosed or misdiagnosed. Purpose: Review and summarize recent diagnostic updates and emergent intervention strategies for acute and chronic mesenteric ischemia. Methods: A narrative review of all relevant studies from January 2022 through September 2023. Results: A total of 11 studies from MEDLINE, supplemented with 44 studies …

Nrf2 Activation In Trp53;P16-Deficient Mice Drives Oral Squamous Cell Carcinoma, Samera H Hamad, Rani S Sellers, Nathan Wamsley, Paul Zolkind, Travis P Schrank, Michael B Major, Bernard E Weissman

2020-Current year OA Pubs

UNLABELLED: Aberrant activation of the NRF2/NFE2L2 transcription factor commonly occurs in head and neck squamous cell carcinomas (HNSCC). Mouse model studies have shown that NRF2 activation alone does not result in cancer. When combined with classic oncogenes and at the right dose, NRF2 activation promotes tumor initiation and progression. Here we deleted the tumor suppressor genes p16INK4A and p53 (referred to as CP mice), which are commonly lost in human HNSCC, in the presence of a constitutively active NRF2E79Q mutant (CPN mice). NRF2E79Q expression in CPN mice resulted in squamous cell hyperplasia or dysplasia with hyperkeratosis in the esophagus, oropharynx, …

Recent Advances And Applications Of Nanostructured Membranes In Water Purification, Didem Aydin, İlkay Hilal Gübbük, Mustafa Ersöz

Turkish Journal of Chemistry

In recent years, water pollution caused by hazardous materials such as metals, drugs, pesticides, and insecticides has become a very serious environmental and health problem that needs to be addressed urgently. The nutritional needs associated with the increasing population also increase the demand for water use and rapidly increase the rate of freshwater consumption. Since most of the water in the universe is in the form of sea water, which cannot be directly used, freshwater resources are limited ,compared to the existing available water. When addressing the purification of all kinds of pollution in environmental research, nanostructured membranes attract attention …

Sustainable Bio-Based Solid Phase Extraction Adsorbent For The Determination Of Various Types Of Organic Compounds, Mustafa Zafer Ozel, James H. Clark, Avtar S. Matharu

Turkish Journal of Chemistry

A sustainable, bio-based, mesoporous material, Starbon A800, was explored for use as an adsorbent in solid phase extraction (SPE). A solution containing seven nitrosamines was first used as a standard to optimise conditions for extraction efficiency with Starbon A800. After optimising conditions, 25 compounds of varying polarity (terpenes, phenolics, pesticides, PAHs, amines, and nitrosamines) were extracted with SPE using either Starbon® A800, C18 or Porous Graphitic Carbon (PGC) as the adsorbent, for comparison purposes. At the same time, 3 different elution solvents (heptane, dichloromethane, and ethanol) were used for each type of adsorbent. Hansen solubility parameters can be used to …

Cover And Contents

Turkish Journal of Chemistry

No abstract provided.

N-(2-Aminobenzoyl)Benzotriazole Mediated Synthesis Of 3- Acyl-2-Alkyl(Aryl)-4-Hydroxyquinolines And 3-Acylamino-4(3h) Quinazolinones, İlbilge Merve Şenol, Sevtem Gökbulut Satioğlu, İlhami Çeli̇k

Turkish Journal of Chemistry

New methods have been developed for the synthesis of the substituted quinolines and quinazolinones derivatives by utilizing N-(2-aminobenzoyl)benzotriazoles under mild reaction conditions. 3-Acyl-2-alkyl(aryl)-4-hydroxyquinolines were obtained in modarete yields by the reaction of N-(2-aminobenzoyl)benzotriazoles and diketones in the presence of tert-BuOK. 3-Acylamino-4(3H) quinazolinones were obtained in good yields via N-(2-aminobenzoyl)benzotriazoles, orthoester and hyrazides in one-pot.

Structure-Activity Relationship Of Anticancer Drug Candidate Quinones, Nadire Özenver, Neslihan Sönmez, Merve Yüzbaşıoğlu Baran, Ayşe Uz, Lütfiye Ömür Demi̇rezer

Turkish Journal of Chemistry

Breast cancer is one of the most prevalent cancer types worldwide. Chemotherapy is a substantial approach in the management of breast cancer despite the occurrence of chemotherapy-associated side effects and the development of multidrug resistance in cancer cells. At this point, a variety of quinone derivatives may represent potential as possible anticancer drug candidates due to possessing structural similarity towards clinically used anticancer drugs like doxorubicin. Therefore, we investigated the cytotoxic effects of various quinone derivatives with structural diversity towards a variety of breast cancer cells. We further determined their toxicity in healthy cells to evaluate their drug capability potential. …

Synthesis And Characterization Of Surface-Modified Magnetic Mesoporous Silicate Materials For Phosphate Adsorption, Ayşegül Gençer, Burcu Uysal Karataş, Önder Topel, Nadir Ki̇raz

Turkish Journal of Chemistry

The magnetic mesoporous silica material, Mag-MCM-41, was synthesized by coating magnetite (Fe3O4) nanoparticles with a mesoporous material called MCM-41. Mag-MCM-41 and modified nanomaterials Mag-MCM-41-NN and Mag-MCM-41-NN-Fe+3 which were modified with aminopropyl functional groups. In water and wastewater, phosphate anions are considered significant contaminants due to their detrimental impact on the environment. They promote the growth of algae, leading to eutrophication. The purpose of this study is to investigate the removal of phosphate anions from aqueous solutions using modified magnetic silica particles. The Mag-MCM-41 material exhibits hexagonal properties and belongs to the class of “mesoporous” materials. It has a surface area …

An Efficient One-Pot And Simple Multicomponent Approach To The Synthesis Of Highly Functionalized Furans Containing Dialkyl Phenol, Fourogh Jalili Milani, Mahmoud Nassiri, Jaber Salehzadeh

Turkish Journal of Chemistry

In this study, arylglyoxals, acetylacetone, and 2,6-dimethyl phenol or 2,6-di-tert-butyl phenol are combined to efficiently synthesize a series of 1-(4-(3,5-dialkylphenyl)-2-methyl-5-phenylfuran-3-yl) ethan-1-one derivatives in excellent yields. These reactions were carried out in acetone at reflux under catalyst-free conditions in the presence of triethylamine as a base for 3 h. NMR, FT-IR, EI-MS, and elemental studies were used to characterize the products' structural characteristics. The present study has also several benefits, such as excellent yields and the ease of workup procedure, making it an appealing, practical, and acceptable one-pot method for producing functionalized derivatives of dialkyl furan.

Voltammetric Measurement Of Catechol-O-Methyltransferase Inhibitor Tolcapone In The Pharmaceutical Form On The Boron-Doped Diamond Electrode, Musa Kiran, Yavuz Yardim

Turkish Journal of Chemistry

This study presents an electroanalytical approach to measure the catechol-O-methyltransferase (COMT) inhibitor tolcapone (TOL) using a boron-doped diamond (BDD) electrode. The application of cyclic voltammetry (CV) technique revealed that TOL exhibited a distinct, diffusion-controlled, irreversible anodic peak at a potential of approximately +0.71 V (vs. Ag/AgCl) in a 0.1 mol L–1 phosphate buffer solution (PBS) with a pH of 2.5. The oxidation of TOL is highly dependent on the pH and supporting electrolytes. Based on the data obtained from the pH investigation, a proposed mechanism for the electro-oxidation of TOL is suggested. Using the square wave voltammetry (SWV) technique, a …

Alcohol-Mediated Changes In Myoblast Hif-1Α Protein Expression And Relationships With Decreased Differentiation Capacity: A Preliminary Analysis, Colby Newman, Alexandra Khartabil, Matthew Dileo, Zenaida Villalba, Danielle Levitt

International Journal of Exercise Science: Conference Proceedings

Alcohol-mediated myopathy, or decreased functional skeletal muscle (SKM) mass, is highly prevalent among individuals with alcohol use disorder (AUD). SKM regeneration aids in maintaining functional SKM muscle mass. Using an in vitro (myoblast) model, we showed that alcohol (EtOH) treatment decreases differentiation capacity in association with decreased glycolytic function, but the underlying mechanisms are incompletely understood. Hypoxia-inducible factor (Hif)-1α is a transcription factor that promotes glycolysis, the bioenergetic pathway that primarily supports myoblast energetic demands. EtOH can increase or decrease Hif-1α expression in various cell types, but this has not been assessed in myoblasts. PURPOSE: Test the hypothesis that alcohol …

Within-Day Reliability Of Submaximal Running Economy Testing, Garrett M. Oehlert, Eric J. Jones, Dustin P. Joubert

International Journal of Exercise Science: Conference Proceedings

Running economy (RE), a measure of oxygen consumption associated with a given intensity, has been identified as a critical component of endurance performance. Acute, same-day interventions, such as alterations in footwear, have been shown to significantly impact RE. Quantifying the reliability of within-day RE measures would determine minimal detectable differences that could be expected when testing footwear and other acute interventions. PURPOSE: Assess the within-day reliability of RE testing on a calibrated metabolic measurement system. METHODS: Trained male distance runners (n=10) were recruited to perform 4 × 5-minute trials under constant conditions (set speed and in their own …

Salivary C-Reactive Protein And Cortisol Analysis In Collegiate Female Lacrosse Athletes During A 2-Game Week, Kelsi R. Lambright, Stephanie Mathews, Jennifer A. Bunn

International Journal of Exercise Science: Conference Proceedings

Cortisol (CORT) and C-reactive protein (CRP) are two indicators of stress and are linked to tissue inflammation and illness. Increased stress and inflammation have been shown to decrease cognitive functioning, leading to diminished performance for athletes. Subjectively, athletes indicate fluctuations in stress, but few studies have assessed the physiological response of training and competition in elite female athletes. PURPOSE: The primary aim was to assess the acute response of CORT and CRP in Division I female lacrosse athletes during a high-stress week, including two games and three practices during the competitive season. The secondary aim was to assess the …

Impact Of Menstrual Cycle On Resting And Postprandial Metabolism In Recreationally Active, Eumenorrheic Females, Adelle Kanaan, Emma Ware, Erika Zoellner, Catherine Mbango, Deltra Muoki, Wayne Brewer, Mindy Patterson, Derek Miketinas, Siddhartha Angadi, Glenn A. Gaesser, Wesley Tucker

International Journal of Exercise Science: Conference Proceedings

Changes in estrogen and progesterone across the menstrual cycle impact many biological systems including resting and postprandial metabolism. PURPOSE: To investigate whether menstrual cycle phase impacted resting and postprandial energy expenditure (EE) and substrate utilization in recreationally active, eumenorrheic females. METHODS: In this study, 8 eumenorrheic females (mean±SD age: 33±8 years, BMI: 22.5±2.2 kg/m²; VO_2max: 36.9±3.8 ml/kg/min) had resting energy expenditure (REE) and substrate utilization continuously measured with indirect calorimetry for 45 min at rest after an overnight fast, and for 3 h after a mixed meal (490 kcal, 53% carbohydrate, 31% fat, 20% …

Driving Entrainment Of Locomotor And Respiratory Systems To Determine Metabolic Efficiency, Michael T. Young, Jennifer M. Yentes, Kuanting Chen

International Journal of Exercise Science: Conference Proceedings

Many biological rhythms, including breathing and locomotor rhythms, are interconnected through a phenomenon known as coupling. Locomotor respiratory coupling (LRC) is quantified using a ratio of stride cycles to breaths. For example, an entrained LRC ratio of two strides to one breathing cycle, is a typical ratio humans use while walking. While various ratios have been recorded, previous research has suggested that not deviating from a particular ratio is associated with low energy expenditure. However, recent research challenges the assumption that stronger LRC correlates with lower energy expenditure, but rather, LRC variability is associated with lower energy expenditure. In addition, …

Release Of Microrna15a/16 From Skeletal Muscle During Exercise Regulates Proteostasis In Muscle And Cancer, Patrick Ryan, Selina Uranga, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

Proteostasis, the intricate process of maintaining the proteome, is a critical matter for cellular health. The regulation of the multiple intersecting metabolic pathways comprising the proteostatic network is correspondingly complex and incompletely understood; however, a new and intriguing candidate in understanding this process has emerged in the form of microRNA (miRNA), small RNA species which are capable of regulating hundreds of genes at once by binding to and degrading mRNA transcripts. We have previously shown that muscle contraction results in the release of two miRNA species (miR15a/16) from skeletal muscles, and that a perfusate rich in these miRNAs slows growth …

Differences In Skeletal Muscle Oxygenation Of Vastus Laterals And Gastrocnemius During Exhaustive Exercise, Kaitlyn Gingell, Ricardo Torres, Jordan Ledington, Leroy Bolden, Tomas Chapman-Lopez, Dylan Willburn, Meena Meiyyappan, Adam Crone, Panagiotis Koutakis, Jeff S. Forsse

International Journal of Exercise Science: Conference Proceedings

Maximal oxygen uptake (VO2 Max) is utilized to assess cardiorespiratory fitness (CRF) and performance in various populations. Although VO2max is the gold standard for assessing aerobic performance and CRF, the accessibility, time, and cost are limiting factors for frequent evaluation. Recently, skeletal muscle oxygen saturation (SmO2) has been utilized to characterize the amount of oxygen available in skeletal muscle tissue and the efficiency of oxygen uptake and to serve as an alternative metric to assess CRF. However, minimal research has been conducted to observe the difference in SmO2 location placement between the vastus laterals (VL) and gastrocnemius (GS) during exhaustive …

The Transient Influence Of Acute Bouts Of Aerobic Exercise On Vitamind Concentrations In Moderate Stages Of Chronic Kidney Disease, Meena Meiyyappan, Leroy Bolden, Tomas Chapman-Lopez, Ricardo Torres, James K. Taylor, Keri Collins, Crawford Allison, Panagiotis Koutakis, Jeff S. Forsse

International Journal of Exercise Science: Conference Proceedings

Chronic kidney disease (CKD) is a gradual loss of renal filtration and has been shown to be associated with lower levels of vitamin D (Vit-D). Vit-D is highly synthesized in the kidneys and is linked to multiple health conditions. Aerobic exercise of differing intensity and modality has shown potential in acutely improving Vit-D concentration in healthy and diseased populations. Purpose: To determine the influence of acute bouts of high-intensity interval exercise (HIIE) and steady-state exercise (SSE) on Vit-D concentrations in individuals with moderate stages of CKD. Methods: A total of 13 participants (n = 7 women; n = 6 men; …