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Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis By Modulating The Expression Of Apoptotic Genes, Members Of Polycomb Repressive Complex 2, And Levels Of Tri-Methylating Lysine 27 At Histone 3 In Human Malignant Melanoma Cells, Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E. Giova, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis L. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were …

“You Are Not The Same Person You Were:” On Diagnosis Seeking During A Liminal Period And Systemic Lupus Erythematosus, Kaylee A. Appleton

USF Tampa Graduate Theses and Dissertations

“You are the healthiest looking sick person I’ve ever met”

(Daphne, 62)

Systemic Lupus Erythematosus (SLE) is one of many chronic illnesses considered to be “invisible.” Once the onset of symptoms occurs, the illness can drastically change the dynamic of an individual's life in ways that may not be noticeable to others, even before a chronic illness diagnosis. I discuss how these individuals experience the liminal period of developing symptoms and seeking a diagnosis. Furthermore, I discuss vignettes that exemplifies the occurrence of a liminal state while looking at the current for-profit set-up of healthcare in the United States. Utilizing …

Exploring The Swimming Behavior Of A Hydromechanical Copepod In The Context Of Positive Density Dependence, Eva J. Muir

USF Tampa Graduate Theses and Dissertations

This dissertation investigates the swimming behavior and population dynamics of the marine copepod, Acartia tonsa. This copepod is of interest as it utilizes hydromechanical signals within the environment to navigate its surroundings, resulting in a distinctive hop/sink swimming pattern. This adaptive trait serves several purposes including searching for mates, obtaining food, and detecting predators, however the evolution of this unique swimming style has not been explored. To address this knowledge gap, the first chapter of this dissertation explores the swimming characteristics and escape responses of captive-reared and wild-caught A. tonsa. When reared in aquaculture, A. tonsa populations experience higher populations …

A Model-Agnostic Computational Method For Discovering Gene–Phenotype Relationships And Inferring Gene Networks Via In Silico Gene Perturbation, Rastko Stojšin, Xiangning Chen, Zhongming Zhao

Faculty, Staff and Student Publications

Background Deep learning architectures have advanced genotype‒phenotype mappings with precision but often obscure the roles of specific genes and their interactions. Our research introduces a model-agnostic computational methodology, capitalizing on the analytical strengths of deep learning models to serve as biological proxies, enabling interpretation of key gene interactions and their impact on phenotypic outcomes. The objective of this research is to refine the understanding of genetic networks in complex traits by leveraging the nuanced decision-making of advanced models.

Results Testing was conducted across several computational models representing varying levels of complexity trained on gene expression datasets for the prediction of …

Pharmacological Management Of Fragile X Syndrome: A Systematic Review And Narrative Summary Of The Current Evidence, Lance V. Watkins, Seungyoun Moon, Lisa Burrows, Samuel Tromans, Julian Barwell, Rohit Shankar

Peninsula Medical School

Introduction: Fragile X syndrome (FXS) is the most common inherited cause of Intellectual Disability. There is a broad phenotype that includes deficits in cognition and behavioral changes, alongside physical characteristics. Phenotype depends upon the level of mutation in the FMR1 (fragile X messenger ribonucleoprotein 1) gene. The molecular understanding of the impact of the FMR1 gene mutation provides an opportunity to target treatment not only at symptoms but also on a molecular level. Methods: We conducted a systematic review to provide an up-to-date narrative summary of the current evidence for pharmacological treatment in FXS. The review was restricted to randomized, …

Single Cell-Transcriptomic Analysis Informs The Lncrna Landscape In Metastatic Castration Resistant Prostate Cancer, Debanjan Saha, Ha X Dang, Meng Zhang, David A Quigley, Felix Y Feng, Christopher A Maher

2020-Current year OA Pubs

Metastatic castration-resistant prostate cancer (mCRPC) is a lethal form of prostate cancer. Although long-noncoding RNAs (lncRNAs) have been implicated in mCRPC, past studies have relied on bulk sequencing methods with low depth and lack of single-cell resolution. Hence, we performed a lncRNA-focused analysis of single-cell RNA-sequencing data (n = 14) from mCRPC biopsies followed by integration with bulk multi-omic datasets. This yielded 389 cell-enriched lncRNAs in prostate cancer cells and the tumor microenvironment (TME). These lncRNAs demonstrated enrichment with regulatory elements and exhibited alterations during prostate cancer progression. Prostate-lncRNAs were correlated with AR mutational status and response to treatment with …

Fast And Slow: Recording Neuromodulator Dynamics Across Both Transient And Chronic Time Scales, Pingchuan Ma, Peter Chen, Elizabeth I Tilden, Samarth Aggarwal, Anna Oldenborg, Yao Chen

2020-Current year OA Pubs

Neuromodulators transform animal behaviors. Recent research has demonstrated the importance of both sustained and transient change in neuromodulators, likely due to tonic and phasic neuromodulator release. However, no method could simultaneously record both types of dynamics. Fluorescence lifetime of optical reporters could offer a solution because it allows high temporal resolution and is impervious to sensor expression differences across chronic periods. Nevertheless, no fluorescence lifetime change across the entire classes of neuromodulator sensors was previously known. Unexpectedly, we find that several intensity-based neuromodulator sensors also exhibit fluorescence lifetime responses. Furthermore, we show that lifetime measures in vivo neuromodulator dynamics both …

Investigations Of The Eutectic Formation And Skin Rejuvenation By Hyaluronan - Kojic Acid Dipalmitate System, Syed Waqar Hussain Shah, Sumbal Imran, Iram Bibi, Kashif Ali, Nadia Bashir

Karbala International Journal of Modern Science

Eutectic phenomenon has been investigated in binary system based on biopolymer hyaluronan (HN) and kojic acid dipalmitate (KAD). Solid-liquid phase diagram showed a significant dependence of melting points on weight fraction of KAD up to KAD < 0.5. A negligible regain to melting temperature of pure KAD occurred later. Simulations of molecular mechanics using a four-unit segment of HN and KAD revealed the interaction between carbonyl of KAD with 4-OH on N-acetylglucosamine unit of oligomer. Infrared vibrational spectroscopy also endorsed the existence of a weakly interacting system. Such behavior was expected due to steric hinderance and rigidity of biopolymer. The thermal decomposition temperature of HN (i.e., 215 °C) was increased to 322 °C in HK50 having HN and KAD in 1:50 w/w. Bioelectric impedance analysis revealed that these green materials could promote skin health in humans.

Synthesis And Characterization Of Renewable Heterogeneous Catalyst Zno Supported Biogenic Silica From Pineapple Leaves Ash For Sustainable Biodiesel Conversion, Nadila Pratiwi, Suriati Eka Putri, Yulia Shinta, Arya Ibnu Batara, Diana Eka Pratiwi, Abd Rahman, Nur Ahmad, Heryanto Heryanto

Karbala International Journal of Modern Science

This study reports on the first case of the low-cost and environmentally friendly ZnO/SiO2 heterogeneous catalyst from pineapple leaves ash (PLA). Catalyst shows excellent performance in catalyzing the transesterification of waste cooking oil (WCO) with methanol for biodiesel conversion. This study focuses on assessing the influence of Zn content on physicochemical characteristics, using XRD, FTIR, SEM, and N2 adsorption-desorption methods. In addition, three different Zn content levels (20, 25, and 30 %wt) were applied. The results showed that all ZnO/SiO2 samples exhibited characteristics suitable for use as catalyst with an average crystallite size of 31.83-34.15 nm, and a surface area …

Surprised By Similarity: C.S. Lewis And Thomas Merton On The Self, John M. Gillespie

Sehnsucht: The C. S. Lewis Journal

Although they never corresponded, and they never met, the Anglican professor C.S. Lewis and the Trappist monk Thomas Merton nevertheless wrote in strikingly similar terms of the “true self,” or a person redeemed by Christ, and a “false self,” the rebellious person one believes themselves to be, but is not. There is very little academic work on the confluence of Lewis and Merton, and none thus far that specifically addresses their shared concept of the self. The aim of this paper is to address this gap in scholarship and, through an intertextual investigation of each man’s major works, fill it. …

Breeding Cool-Season Forage Grasses For A Warming Climate, D. P. Malinowski, J. Ford, A. Stewart, D. Woodfield, W. E. Pinchak

IGC Proceedings (1977-2023)

In many parts of the world, changing climatic conditions are resulting in increased temperatures and more variable precipitation, intensifying the duration and severity of drought, especially in summer. Warming climate is considered one reason for the increasing failure of traditional, summer-active cool-season perennial grasses at the margin of their zone of adaptation in naturally C4 grass-dominated ecosystems of the Southern Great Plains of the USA. Two cool-season perennial forage grasses orchardgrass (Dactylis glomerata L.) and tall fescue (Lolium arundinaceum (Schreb.) Darbysh.) are of major economic and ecological importance in these regions. In 2008, we initiated a breeding program …

Invasive Annual Grass Issues In Canadian Rangelands, V. Maloney

IGC Proceedings (1977-2023)

It has been understood for several years now that Canadian grasslands are among the world’s most endangered ecosystems. While the endangerment of the world’s coral reefs and rainforests may make the news more often, the increasingly fast decline of native Canadian grasslands is having a major impact on species diversity as well as global carbon supplies. In this paper, I will discuss the impact invasive annual grasses have had on two important rangeland ecosystems in Canada. Furthermore, strategies for protecting and restoring these invaluable lands will be discussed.

Forage Data Hub – A Platform For Sharing Valuable Datasets For Resilience, Amanda J. Ashworth, L. Marshall, J. J. Volenec, M. Berti, E. Van Santen, C. Williams, V. Gopakumar, J. Foster, V. Picasso, J. Su

IGC Proceedings (1977-2023)

In accord with the necessity to enhance ecosystem services and productivity in food systems, is the increase of data availability at multiple scales and over time. To help meet this need, we discuss the development of a National Forage Data Hub which will act as a platform to curate, share, and analyze data pertaining to forage systems. This centralized hub will leverage existing datasets by bridging multiple sources including forage crop—soil, water, and nutrient availability—yield (animal and crop) potential (and gaps)— climate—management systems at high spatial and temporal resolution enabling system interaction assessments through next-generation analytics. This novel approach to …

Tennessee Master Beef Producer Program Promotes Sustainable Beef Production, K. M. Mason, G. E. Bates, J. D. Rhinehart

IGC Proceedings (1977-2023)

Tennessee is home to 1.75 million beef cattle as of January 2022 (USDA, 2022). The majority of cattle farms in Tennessee are cow-calf operations, with a few stocker-backgrounder operations across the state. Most of the cows in the state are maintained on tall fescue (Schedonorus arundinaceus (Schreb.) Dumort. pastures, with hay being fed in the winter months and sometimes during the summer. Maintenance of a strong cow herd and sufficient grazing land relies on utilization of sustainable production practices. With urban sprawl creating competition for land area, it is important to use production practices that are efficient and attainable …

Guidelines For Optimal Use Of Near Infrared Spectroscopy (Nirs) With Sample Preparation And Presentation Across Instrument Platforms, D. W. Mcintosh

IGC Proceedings (1977-2023)

Guidelines for optimal use of near infrared spectroscopy (NIRS) with sample preparation and presentation across instrument platforms will cover methods and resources specifically for NIRS analysis. Recommendations for sample preprocessing and scanning required to ensure compatibility across calibrations will be highlighted. Proper calibration determinations, monitoring, and performance will be demonstrated to create a baseline for correct use. Additional materials about the use and reporting of nutritive content predictions for publication will be discussed. Goals of this topic session are to develop an understanding of the basics of NIRS technology and provide the basics to what a laboratory is required to …

Challenges And Opportunities For Revegetation In Areas Dominated By Invasive Annual Grasses, B. A. Mealor, J. A. Crose

IGC Proceedings (1977-2023)

Invasive annual grasses (IAG) are a primary ecological threat to sustainability and ecological integrity of rangelands in the western U.S. While availability of effective tools to control IAG is increasing, ensuring compatibility of control practices with other management practices, such as seeding desirable species, is a critical information need. We summarize a series of studies investigating influences of timing, seeding depth, species selection, and planting timing with various herbicides in sites dominated by invasive annual grasses.

Transitioning To Multiple-Use Polyculture Grassland-Derived Bioenergy Feedstock Systems, J. P. Muir, J. L. Foster, A. Falk, S. Weiss, W. D. Pitman, W. Alsion, H. D. Naumann, J. R. Bow, C. Shadrock, E. Osei, O. Lasater

IGC Proceedings (1977-2023)

We argue that research and development of grassland-derived bioenergy feedstock (GBF) has focused unduly on dedicated monospecific biomass systems to the detriment of more stable multiple-use multispecies grasslands. This has retarded GBF adoption as a viable, sustainable contribution to renewable energy production in North America. We hypothesize that focusing on multiple-use GBFs will foster greater feedstock availability for nascent renewable energy generation while minimizing financial risk to grassland husbandry during market transitions from current grazing systems to more flexible business models. Our hypothesis is that source and demand are more likely to develop simultaneously under less risky multiple-use grassland management. …

Observing And Simulating Temperate Grasslands In Central Europe, C. Nendel

IGC Proceedings (1977-2023)

This presentation summarises recent activities to observe grassland features using remote sensing and uses this data to feed mechanistic simulation models for temperate grassland vegetation in Central Europe, in order to assess underlying processes that are difficult to observe. Public interest has recently focused on grassland ecosystem services, such as carbon stocks, nitrate retention and greenhouse gas emissions; variables that in principle can be simulated using models. However, current grassland models suffer from the fact that species dynamics in grasslands are very active, and may change in response to water supply and management. As different species come with different traits, …

The Effect Of Nitrogen Rates And Plant Density On Grain Yield Components And Persistence In Intermediate Wheatgrass (Thinopyrum Intermedium) And Mountain Rye (Secale Strictum), M. T. Newell, N. Munday, R. C. Hayes

IGC Proceedings (1977-2023)

Intermediate wheatgrass (IWG; Thinopyrum intermedium) and Mountain Rye (Mtn Rye; Secale strictum) have potential for release as dual-purpose (grazing and grain production) perennial grains in Australia due to their superior longevity compared to hybrid perennial wheats. Initially developed as perennial forage grasses, few management guidelines exist to inform agronomic practices to maximise grain yields and profitability in Australian environments. An experiment was established in 2020 to examine the effect of plant density and nitrogen rates on grain yield components. The experiment compared the two species (IWG, Mtn Rye) sown at three plant densities (50, 100 and 200 plants/m2) …

Participatory Evaluation Of Urochloa (Brachiaria) Grasses In The Cool Highlands Of Central Kenya, Elkana Nyambati, W. Ayako, S. K. Mailu

IGC Proceedings (1977-2023)

Grasslands are increasingly threatened by over exploitation, degradation and climate change that has undermined their productive capacity, leading led to inadequate quantity and quality feed. Urochloa grass, a native of eastern Africa has been widely improved and adapted as livestock forage. A farmer participatory study was conducted to evaluate productivity of eight Urochloa grasses (Urochloa brizantha cv. Marandu, U. brizantha cv. Xareas, U. brizantha cv. Piata, U. brizantha cv. MG4, U. decumbens cv. Basilisk, U. humidicola cv. Humidicola, U. humidicola cv. Llanero and Urochloa hybrid Mulato II) compared to controls of Pennisetum purpureum (Napier grass cv. Kakamega 1 …

Is There A Need For Tailored Graduate Programs For International Students?, K. T. Oduor, Lynn E. Sollenberger, J. Dubeux, L. M. D. Queiroz

IGC Proceedings (1977-2023)

International studies often present opportunities for capacity development and mentorship for students to equip them with the knowledge and skills to address the challenges in their home countries. Typically, international graduate students are drawn from diverse educational and cultural backgrounds different from those in their host countries. Adjusting to these changes might be challenging and time-consuming, thus influencing their academic journey. Understanding these challenges might provide international students the opportunity to address them in time and, where possible, seek help. In this paper, we discuss some graduate program-related challenges international students face and provide potential recommendations that might result in …

Sunn Hemp As An Alternative Forage In The Texas High Plains Region, S. A. O’Shaughnessy, H. M. Mosqueda, D. K. Brauer

IGC Proceedings (1977-2023)

Forage production in the Texas High Plains, a semi-arid region, is critical to sustain the local cattle industry. However, the main source of water for irrigation is the highly depleted Ogallala Aquifer, making forage crop water productivity (CWP) of high importance. In this one-year study, three types of forages were cultivated under deficit irrigation treatments of 80% and 50% of full crop water use. The forages were: a non-brown midrib forage sorghum; a legume, sunn hemp; and a sorghum/sunn hemp mix. The experiment was conducted in Bushland, Texas in 2019. Treatment plots were arranged in a split plot design with …

Differences In Carbon Sequestration And Water Use Between A Semi-Arid Native Grassland And Encroaching Vachellia Karroo Woodland, C. Weideman, A. R. Palmer, G. Carolyn, Kathleen Smart

IGC Proceedings (1977-2023)

Tree and shrub encroachment and proliferation in global grasslands and savannas is widely considered to imply a trade-off between ecosystem C and H2O regulating functions, based on the premise that increased C sequestration associated with higher woody biomass ostensibly drive declines in water production through concomitant increases in evapotranspiration (ET), but evidence for this across climatic and environmental gradients is equivocal. To evaluate these claims, we ran a paired eddy covariance experiment in a native semi-arid C4 grassland and adjacent encroaching Vachellia karroo woodland on the eastern seaboard of South Africa and compared ecosystem C and H2O budgets over nearly …

Contribute To A Definition Of Extensive Grazing (Mediterranean), J. Potes, J. P. F. Almeida, C. Matos, A. Teixeira

IGC Proceedings (1977-2023)

The subject of this paper is a definition of Extensive Grazing from Extensive Systems of Animal Production in Mediterranean environments. The edaphoclimatic characterization is essential to understand the low level of primary productivity as a consequence of irregular precipitation over the year and its absence during the long, dry and hot summer. The powerless of soils justify the limited contribution of plants and make clear the necessary recovery of the soil and storage of water naturally (soil) or artificially (irrigation). Considering the IPBES (Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services) definition of extensive grazing it has been used the …

Soil Carbon Monitoring Program For Ranches In Dryland Ecosystems, David E. Prado-Tarango, S. Ates, J. Talbot

IGC Proceedings (1977-2023)

Implementation of ranching practices that lead to greater soil carbon sequestration has become increasingly important due to rising atmospheric CO2 levels. Sustainable ranching practices are suggested to improve soil health and sequester more carbon in the soil. However, there is a paucity of measured data from replicated on-farm studies to support this premise. Thus, we developed a soil carbon monitoring program for a ranch located in the state of Oregon. Our monitoring program is focused on measuring the net carbon budget and the soil carbon sequestration totals. To achieve this goal, we combined field-sampling data with the COMET-Farm model. A …

Answering Complex Questions As We Make Headway On Annual Grasses And Fire, T. Prather, L. Jones, E. Strand, G. Harrison, K. Johnston

IGC Proceedings (1977-2023)

Annual grasses challenge our ability to meet forage production and plant community conservation objectives. Direct competition, potential mutualism, and shortened fire return interval interplay resulting in complexity of how to meet challenges we face. Bromus tectorum presents challenges to sagebrush dominated plant communities with shortened fire return intervals and the advancing invasion of Ventenata dubia poses threats to forage production through competitive interactions that may involve mutualism to enhance V. dubia’s invasion of annual and perennial grass dominated plant communities. Use of fuel breaks can reduce the extent of fires and their proper function hinges on their reduced plant biomass …

Rangeland Devastated By Invasive Annual Grass, H. E. Quicke

IGC Proceedings (1977-2023)

This paper serves as an introduction to the thematic session on protection and restoration of western US rangelands. Exotic annual grass species have steadily invaded western US rangeland for decades. More recently, the problem of invasive annual grasses received national attention with multiple stakeholders working together under the auspices of Western Governors’ Association to develop a common strategy to fight back.

Preventing Conversion To A Highly Degraded Invasive Dominated System, C. V. Ransom

IGC Proceedings (1977-2023)

Invasive annual grasses (IAG) negatively impact rangeland across the Western US. Within the Intermountain Region, downy brome, Japanese brome, medusahead, and ventenata are among the most prevalent invasive annual grass species. Research conducted at Utah State University has addressed numerous approaches to IAG management as well investigating restoration strategies of highly degraded sites. Under harsh climates common to the Western US, restoration has been extremely challenging, causing focus to shift towards prioritizing the protection of existing desirable plant communities. In either restoration or plant community protection approaches, annual rainfall is the largest driver of success. Changes or shifts in plant …

Red Deer Browsing Decelerates Shrub Regrowth Despite Increasing Wolf Presence, F. Riesch, C. Raab, Bettina Tonn, N. Gerber, M. Zetsche, J. Signer, Marcus Meißner, Sven Herzog, Niko Balkenhol, Johannes Isselstein

IGC Proceedings (1977-2023)

Semi-natural open habitats in Europe have been shaped by traditional land use practices, such as extensive mowing or livestock grazing. However, socio-economic transformations have led to the abandonment of many grassland and heathland areas and conservation management is now required to maintain these biodiverse habitats. Grazing by wild red deer (Cervus elaphus) can be a convenient alternative to laborious mechanical management or livestock grazing. Yet it remains unclear if free-ranging ungulates can counteract shrub growth sufficiently to maintain open habitats—especially with natural predators, i.e. wolves (Canis lupus), recolonizing Europe. To assess red deer effects on shrub …

Using Recurrent Phenotypical Selection To Improve Drought Tolerance Of Red Clover, M. Sahin, S. Reid, E. Sahin, S. Ates, S. Elias, David B. Hannaway

IGC Proceedings (1977-2023)

Red clover is one of the most important forage legumes in the world. It is utilized extensively in European and North American farming systems. Approximately, 4 million hectares of red clover are grown globally each year for forage production, soil improvement, and as a pollen and nectar source for pollinators (Riday, 2010). In North America, red clover is widely used in temperate regions for pasture, hay, and silage in combination with grasses for soil improvement and as a pollen and nectar source for bumblebees (Bombus). In the northwest, it is also grown for seed production (Anderson et al., 2016) with …