Digital Commons Network^™

H2a.Z Histone Variants Facilitate Hdaci-Dependent Removal Of H3.3k27m Mutant Protein In Pediatric High-Grade Glioma Cells, Katarzyna B Leszczynska, Francisca N L Vitorino, Benjamin A Garcia, Et Al.

2020-Current year OA Pubs

Diffuse intrinsic pontine gliomas (DIPGs) are deadly pediatric brain tumors, non-resectable due to brainstem localization and diffusive growth. Over 80% of DIPGs harbor a mutation in histone 3 (H3.3 or H3.1) resulting in a lysine-to-methionine substitution (H3K27M). Patients with DIPG have a dismal prognosis with no effective therapy. We show that histone deacetylase (HDAC) inhibitors lead to a significant reduction in the H3.3K27M protein (up to 80%) in multiple glioma cell lines. We discover that the SB939-mediated H3.3K27M loss is partially blocked by a lysosomal inhibitor, chloroquine. The H3.3K27M loss is facilitated by co-occurrence of H2A.Z, as evidenced by the …

Bridging Therapy With Axicabtagene Ciloleucel For Large B-Cell Lymphoma: Results From The Us Lymphoma Car-T Consortium, Michael D Jain, Miriam T Jacobs, Feng Gao, Armin Ghobadi, Et Al.

2020-Current year OA Pubs

During the manufacturing period of autologous chimeric antigen receptor (CAR) T-cell therapy, patients may experience a decline in their condition due to cancer progression. In this study, we investigated the impact of bridging therapy (BT) on the outcome of patients with relapsed/refractory large B-cell lymphoma who received antilymphoma treatment between leukapheresis and axicabtagene ciloleucel (axi-cel) infusion. We conducted our analysis using data from the multicenter US Lymphoma CAR-T Consortium, with a median follow-up of 33 months (range, 4.3-42.1). Out of the 298 patients who underwent leukapheresis, 275 patients received axi-cel. A total 52% of patients (n = 143) who received …

Tnfr1 Signaling Is Positively Regulated By Jak-2 And C-Src Via Tyrosine Phosphorylation, Fatma Zehra Hapi̇l Zevkli̇ler, Fatma Ece Çopuroğlu, Mustafa Gökhan Ertosun, Ufuk Mert, Derya Özeş, Osman Nidai Özeş

Turkish Journal of Biology

Background/aim: Tumor necrosis factor alpha (TNFα, a.k.a. TNF) is a pleiotropic cytokine that exerts most of its effects through type 1 TNF receptor (TNFR1). Following TNF binding, TNFR1 recruits TRADD (tumor necrosis factor receptor type 1-associated DEATH domain). This interaction triggers formation of signalosome complexes which have been claimed to induce apoptosis (via downstream caspase activations), inflammation (via NF-kappaB) and stress pathways (JNK & p38). However, the mechanism underlying TNF-induced ERK and AKT activation is not completely revealed. TNFR1 is known to constitutively bind c-Src and JAK2, and these enzymes were previously demonstrated to modulate TNF signaling. Therefore, we hypothesized …

Prognostic Gene Biomarkers For C-Src Inhibitor Si162 Sensitivity In Melanoma Cells, Seyhan Türk, Ayşegül Yilmaz, Ümit Yavuz Malkan, Gülberk Uçar, Can Türk

Turkish Journal of Biology

Background/aim: Early detection and treatment are crucial in combating malignant melanoma. Src is an important therapeutic target in melanoma due to its association with cancer progression. However, developing effective Src-targeting drugs remains challenging and personalized medicine relies on biomarkers and targeted therapies for precise and effective treatment. This study focuses on Si162, a newly synthesized c-Src inhibitor, to identify reliable biomarkers for predicting Si162 sensitivity and explore associated biological characteristics and pathways in melanoma cells. Materials and methods: Primary melanoma cells (M1, M21, M24, M84, M133, M307, and M2025) were obtained from patients diagnosed with melanoma. Si162 cytotoxicity tests were …

Expression Patterns Of M6a Rna Methylation Regulators Under Apoptotic Conditions In Various Human Cancer Cell Lines, Azime Akçaöz Alasar, Buket Sağlam, İpek Erdoğan Vatansever, Bünyamin Akgül

Turkish Journal of Biology

Background/aim: Cancer is a complex disease that involves both genetic and epigenetic factors. While emerging evidence clearly suggests that changes in epitranscriptomics play a crucial role in cancer pathogenesis, a comprehensive understanding of the writers, erasers, and readers of epitranscriptomic processes, particularly under apoptotic conditions remains lacking. The aim of this study was to uncover the changes in the expression of m6A RNA modifiers under apoptotic conditions across various cancer cell lines. Materials and methods: Initially, we quantified the abundance of m6A RNA modifiers in cervix (HeLa and ME180), breast (MCF7 and MDA-MB-231), lung (A549 and H1299), and colon (CaCo2 …

Circrna Ldlr Promotes Proliferation And Aerobic Glycolysis Of Gastric Cancer Cells By Targeting Chd1 With Mir-449b-5p, Fanye Zeng, Juntao Zhao, Mengting Tong, Wenting He, Nan Li, Yuxiang Fan, Yanhua Zhu, Liping Zhang, Hongliang Zhang

Turkish Journal of Biology

Background/aim: Circular RNAs can serve as detection biomarkers and therapeutic targets for tumors. Our study aimed to elucidate the mechanisms associated with circRNA LDLR (circLDLR) in gastric cancer (GC) proliferation and aerobic glycolysis. Materials and methods: Expression signatures of circLDLR, miR-449b-5p, and CHD1 were examined in GC samples using quantitative PCR. Proliferation ability of MKN-45 cells was assessed via CCK-8 and EdU assays, and cell apoptosis was measured by flow cytometry. Glucose uptake, lactate production, ATP/ADP ratios, and NAD+/NADH ratios in cell supernatants were quantified to evaluate aerobic glycolysis. Subcellular isolation assay, quantitative PCR, immunoblot analysis, RNA immunoprecipitation (RIP), and …

Beneficial Effects Of A Novel Polyherbal Formulation On The Skeletal Muscle Antioxidant Status, Inflammation, And Muscle-Signaling Proteins In Exercised Rats, Mehmet Tuzcu, Oğuzhan Özdemi̇r, Cemal Orhan, Nurhan Şahi̇n, Abhijeet Morde, Muralidhara Padigaru, Prakash Bhanuse, Kazım Şahi̇n

Turkish Journal of Biology

Background/aim: Exhausting exercise can damage muscle tissue due to free radical interactions. It is hypothesized that the increase in free radicals following muscle injury, either due to oxidative damage to biomolecules or the activation of inflammatory cytokines, may lead to secondary muscle damage. This study investigated the effects of a novel joint health formula (JHF) containing bisdemethoxycurcumin-enriched curcumin, 3-O-Acetyl-11-keto-beta-boswellic acid-enriched Boswellia (AKBA), and Ashwagandha on exhaustion time, grip strength, antioxidant status, and muscle-signaling proteins in exhaustively exercised rats. Materials and methods: Twenty-eight rats were divided into four groups: Control (C), exercise (E), E + JHF 100 (100 mg/kg), and E …

Effect Of Thymol On Oxidative Stress And Reelin Signaling Pathway In Alzheimer's Disease Model, Barış Bi̇tmez, Burcu Çevreli̇, Emel Kaşikçi

Turkish Journal of Biology

Background/aim: The purpose of this study was to investigate how thymol affects cognitive functions and the levels of MDA, GSH, Aβ1-42, ApoE, reelin, and LRP8 in an AD model induced in male Wistar albino rats with the application of D-galactose (D-gal) and aluminum chloride (AlCl3). Materials and methods: In this work, 3-month-old male Wistar albino rats were used. Group 1 served as the Control, Group 2 received 0.5 mL/day saline + 0.5 mL/day sunflower oil, Group 3 was administered 200 mg/kg/day AlCl3 + 60 mg/kg/day D-gal, Group 4 received 30 mg/kg/day thymol, and Group 5 was administered 200 mg/kg/day AlCl3 …

Divergent Proteomic Profiles Of Opium Poppy Cultivars, Setenay Aykanat, Mine Türktaş

Turkish Journal of Biology

We examined the proteomic profiles of three registered opium poppy cultivars (Papaver somniferum L.) having different alkaloid contents. The study was conducted on both the stem and capsule organs. A high number of differentially expressed proteins (DEPs) were identified between the cultivars and the organs. We analyzed DEPs for their contribution in GO terms and KEGG pathways. The up-regulated DEPs were significantly enriched in photosynthesis and translation, for morphine-rich and noscapine-rich cultivars, respectively. The data depicted that photosynthesis is crucial for Benzylisoquinoline alkaloid (BIA) biosynthesis, but different processes are also effective on morphine and noscapine biosynthesis, which occur at different …

Self-Managment And Breastfeeding In Women With Preexisting Type 2 Diabetes Mellitus From Thailand, Ratchanok Phonyiam

Dissertations

The prevalence of type 2 diabetes mellitus (T2DM) is rising at a younger age, leading to an increased number of pregnant women with preexisting T2DM. Although traditional beliefs and practices influence diabetes management and breastfeeding, recommendations integrating Thai cultural beliefs in maternal care are lacking. The purpose of this dissertation is to describe diabetes self-management in pregnancy and breastfeeding experiences in women with preexisting T2DM from Thailand. This dissertation is a convergent parallel mixed-methods study, guided by the National Institute on Minority Health and Health Disparities (NIMHD) Framework. Data were collected two times. The first time was during pregnancy (T1). …

Gamma Delta T Cells In Acute Myeloid Leukemia: Biology And Emerging Therapeutic Strategies, Adishwar Rao, Akriti Agrawal, Gautam Borthakur, Venkata Lokesh Battula, Abhishek Maiti

Faculty, Staff and Student Publications

γδ T cells play an important role in disease control in acute myeloid leukemia (AML) and have become an emerging area of therapeutic interest. These cells represent a minor population of T lymphocytes with intrinsic abilities to recognize antigens in a major histocompatibility complex-independent manner and functionally straddle the innate and adaptive immunity interface. AML shows high expression of phosphoantigens and UL-16 binding proteins that activate the Vδ2 and Vδ1 subtypes of γδ T cells, respectively, leading to γδ T cell-mediated cytotoxicity. Insights from murine models and clinical data in humans show improved overall survival, leukemia-free survival, reduced risk of …

Dynamics Of Nanocluster Aerosol In The Indoor Atmosphere During Gas Cooking, Satya S. Patra, Jinglin Jiang, Xiaosu Ding, Chunxu Huang, Emily K. Reidy, Vinay Kumar, Paige Price, Connor Keech, Gerhard Steiner, Philip Stevens, Nusrat Jung, Brandon E. Boor

Purdue University Libraries Open Access Publishing Fund

Nanocluster aerosol (NCA: particles in the size range of 1–3 nm) are a critically important, yet understudied, class of atmospheric aerosol particles. NCA efficiently deposit in the human respiratory system and can translocate to vital organs. Due to their high surface area-to-mass ratios, NCA are associated with a heightened propensity for bioactivity and toxicity. Despite the human health relevance of NCA, little is known regarding the prevalence of NCA in indoor environments where people spend the majority of their time. In this study, we quantify the formation and transformation of indoor atmospheric NCA down to 1 nm via high-resolution online …

Psychological/Behavioral Interventions For Emerging Adults With Chronic Pain, Michael R. Foy, Sandra Kechichian, Judith G. Foy, Maisa Ziadni

Psychological Science Faculty Works

Background: Emerging adults, of whom significant numbers report chronic pain, are characterized as having unique needs and challenges. Psychological/behavioral treatments found to be beneficial for reducing pain outcomes in children and adults are understudied in emerging adults. Following a systematic review of the literature, our objective is to report on quantitative studies of psychological/behavioral interventions for chronic pain in emerging adults. Method: We conducted a search of six databases (Cochrane Central Register of Controlled Trials, Google Scholar, ProQuest, PsycINFO, PubMed, and Web of Science) and reference sections in dissertations and systematic reviews to 4/29/2023. Keywords and phrases were search term …

Association Of Plasma Ykl-40 With Mri, Csf, And Cognitive Markers Of Brain Health And Dementia, Matthew P Pase, Jayandra J Himali, Raquel Puerta, Alexa S Beiser, Mitzi M Gonzales, Claudia L Satizabal, Qiong Yang, Hugo J Aparicio, Daniel J Kojis, Charles S Decarli, Oscar L Lopez, Will Longstreth, Vilmundur Gudnason, Thomas H Mosley, Joshua C Bis, Alison Fohner, Bruce M Psaty, Mercè Boada, Pablo García-González, Sergi Valero, Marta Marquié, Russell Tracy, Lenore J Launer, Agustín Ruiz, Myriam Fornage, Sudha Seshadri

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVES: Higher YKL-40 levels in the CSF are a known biomarker of brain inflammation. We explored the utility of plasma YKL-40 as a biomarker for accelerated brain aging and dementia risk.

METHODS: We performed cross-sectional and prospective analyses of 4 community-based cohorts in the United States or Europe: the Age, Gene/Environment Susceptibility-Reykjavik Study, Atherosclerosis Risk in the Communities study, Coronary Artery Risk Development in Young Adults study, and Framingham Heart Study (FHS). YKL-40 was measured from stored plasma by a single laboratory using Mesoscale Discovery with levels log transformed and standardized within each cohort. Outcomes included MRI total …

A Qualitative Examination Of Teachers’ Experiences Building Student Resilience In Rural Kenya, Priscilla Ann Stafford Allen

Walden Dissertations and Doctoral Studies

Students in rural and underresourced school settings often perform poorly due to adversity. This is evident in subcounty schools where challenges such as domestic violence and low socioeconomic status further impact students' academic performance. Researchers have demonstrated that the relationship between students and teachers is instrumental in building resilience and developing social emotional competence. By fostering resilience, teachers can play a crucial role in mitigating these challenges. However, few studies have examined teacher experience in building resilience in students through a qualitative study. Using a generic qualitative descriptive study and grounded in the theory of resilience, semistructured interviews were completed …

Extrasynaptic Localization Is Essential For Α5gabaa Receptor Modulation Of Dopamine System Function, Alexandra M. Mccoy, Thomas D. Prevot, Md Yeunus Mian, Dishary Sharmin, Adeeba N. Ahmad, James M. Cook, Etienne L. Sibille, Daniel J. Lodge

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

Dopamine system dysfunction, observed in animal models with psychosis-like symptomatology, can be restored by targeting gamma-aminobutyric acid type A receptors (GABAARs) containing the α5, but not α1, subunit in the ventral hippocampus (vHipp). The reason for this discrepancy in efficacy remains elusive; however, one key difference is that gamma-aminobutyric acid type A receptors containing the α1 subunit (α1GABAARs) are primarily located in the synapse, whereas gamma-aminobutyric acid type A receptors containing the α5 subunit (α5GABAARs) are mostly extrasynaptic. To test whether receptor location is responsible for this difference in efficacy, we injected an siRNA into the vHipp to knock down …

Rbpms Regulates Cardiomyocyte Contraction And Cardiac Function Through Rna Alternative Splicing, Peiheng Gan, Zhaoning Wang, Svetlana Bezprozvannaya, John R Mcanally, Wei Tan, Hui Li, Rhonda Bassel-Duby, Ning Liu, Eric N Olson

Faculty, Staff and Student Publications

Aims: RNA binding proteins play essential roles in mediating RNA splicing and are key post-transcriptional regulators in the heart. Our recent study demonstrated that RBPMS (RNA binding protein with multiple splicing) is crucial for cardiac development through modulating mRNA splicing, but little is known about its functions in the adult heart. In this study, we aim to characterize the post-natal cardiac function of Rbpms and its mechanism of action.

Methods and results: We generated a cardiac-specific knockout mouse line and found that cardiac-specific loss of Rbpms caused severe cardiomyocyte contractile defects, leading to dilated cardiomyopathy and early lethality in adult …

Bridging Therapy With Axicabtagene Ciloleucel For Large B-Cell Lymphoma: Results From The Us Lymphoma Car-T Consortium, Michael D Jain, Miriam T Jacobs, Feng Gao, Loretta J Nastoupil, Jay Y Spiegel, Yi Lin, Saurabh Dahiya, Matthew Lunning, Lazaros Lekakis, Patrick Reagan, Olalekan Oluwole, Joseph Mcguirk, Abhinav Deol, Alison R Sehgal, Andre Goy, Brian T Hill, Charalambos Andreadis, Javier Munoz, Julio C Chavez, N Nora Bennani, Aaron P Rapoport, Julie M Vose, David Miklos, Sattva S Neelapu, Frederick L Locke, Armin Ghobadi

Faculty, Staff and Student Publications

During the manufacturing period of autologous chimeric antigen receptor (CAR) T-cell therapy, patients may experience a decline in their condition due to cancer progression. In this study, we investigated the impact of bridging therapy (BT) on the outcome of patients with relapsed/refractory large B-cell lymphoma who received antilymphoma treatment between leukapheresis and axicabtagene ciloleucel (axi-cel) infusion. We conducted our analysis using data from the multicenter US Lymphoma CAR-T Consortium, with a median follow-up of 33 months (range, 4.3-42.1). Out of the 298 patients who underwent leukapheresis, 275 patients received axi-cel. A total 52% of patients (n = 143) who received …

Differential Upregulation Of Th1/Th17-Associated Proteins And Pd-L1 In Granulomatous Mycosis Fungoides, Mario L Marques-Piubelli, Jesus Navarrete, Debora A Ledesma, Courtney W Hudgens, Rossana N Lazcano, Ali Alani, Auris Huen, Madeleine Duvic, Priyadharsini Nagarajan, Phyu P Aung, Ignacio I Wistuba, Jonathan L Curry, Roberto N Miranda, Carlos A Torres-Cabala

Faculty, Staff and Student Publications

Granulomatous Mycosis Fungoides (GMF) is a rare form of mycosis fungoides (MF) characterized by a granulomatous infiltrate associated with the neoplastic lymphoid population and is considered to have a worse prognosis compared with regular MF. The upregulation of the T helper (Th) axis, especially Th17, plays an important role in the pathogenesis of several inflammatory/infectious granulomatous cutaneous diseases, but its role in GMF is still not elucidated to date. In this study, we evaluated the immunohistochemical expression of Th1 (Tbet), Th2 (GATA-3), Th17 (RORγT), T regulatory (Foxp3), and immune checkpoint (IC) (PD-1 and PD-L1) markers in a cohort of patients …

At Chdi Conference, Advocates Inspire Acceleration Of Quest For Huntington’S Disease Therapies, Kenneth P. Serbin Phd

At Risk for Huntington's Disease

No abstract provided.

A Global Database Of Dissolved Organic Matter (Dom) Concentration Measurements In Coastal Waters (Coastdom V1), Christian Lønborg, Cátia Carreira, Gwenaël Abril, Susana Agustí, Valentina Amaral, Agneta Andersson, Javier Arístegui, Punyasloke Bhadury, Mariana B. Bif, Alberto V. Borges, Steven Bouillon, Maria L. I. Calleja, Luiz C. Cotovicz, Stefano Cozzi, Maryló Doval, Carlos M. Duarte, Bradley Eyre, Cédric G. Fichot, E. Elena García-Martín, Alexandra Garzon-Garcia, Michele Giani, Rafael Gonçalves-Araujo, Renee Gruber, Dennis A. Hansell, Fuminori Hashihama, Ding He, Johnna M. Holding, William R. Hunter, J. Severino P. Ibánhez, Valeria Ibello, Shan Jiang, Guebuem Kim, Katja Klun, Piotr Kowalczuk, Atsushi Kubo, Choon-Weng Lee, Cláudia B. Lopes, Federica Maggioni, Paolo Magni, Celia Marrase, Patrick Martin, S. Leigh Mccallister, Roisin Mccallum, Patricia M. Medeiros, Xosé A. G. Morán, Frank E. Muller-Karger, Allison Myers-Pigg, Marit Norli, Joanne M. Oakes, Helena Osterholz, Hyekyung Park, Maria L. Paulsen, Judith A. Rosentreter, Jeff D. Ross, Digna Rueda-Roa, Chiara Santinelli, Yuan Shen, Eva Teira, Tinkara Tinta, Guenther Uher, Masahide Wakita, Nicholas Ward, Kenta Watanabe, Yu Xin, Youhei Yamashita, Liyang Yang, Jacob Yeo, Huamao Yuan, Qiang Zheng, Xosé A. Álvarez-Salgado

Research outputs 2022 to 2026

Measurements of dissolved organic carbon (DOC), nitrogen (DON), and phosphorus (DOP) concentrations are used to characterize the dissolved organic matter (DOM) pool and are important components of biogeochemical cycling in the coastal ocean. Here, we present the first edition of a global database (CoastDOM v1; available at 10.1594/PANGAEA.964012, Lonborg et al., 2023) compiling previously published and unpublished measurements of DOC, DON, and DOP in coastal waters. These data are complemented by hydrographic data such as temperature and salinity and, to the extent possible, other biogeochemical variables (e.g. chlorophyll a, inorganic nutrients) and the inorganic carbon system (e.g. dissolved inorganic carbon …

Relative Periodicity Of Empirical Audio Samples With Application To Dissonance Perception, Linden Faye, Spencer Kuhn, Anil Venkatesh

LASER Journal

The concept of dissonance in music perception has been variously associated with the physical concepts of roughness, instability, and tension by appealing to a subjective cross-sensory analogy. For all the subjectivity around the term, it is noteworthy that non-musical test subjects in clinical experiments have produced remarkably consistent rank-orderings of musical sounds, according to the perceived dissonance of those sounds. Further studies in psychology, neuroscience, and mathematics show that consonance perception appears to be influenced not only by convention and culture but by the psychoacoustics of sound perception, likely by some combination of the Helmholtz theory of roughness and the …

What Waterfowl Hunters Want: Exploring Heterogeneity In Hunting Trip Preferences, Katherine A. Sainsbury, Howard W. Harshaw, David C. Fulton, Nicholas W. Cole, Ashley A. Dayer, Jennie N. Duberstein, Andrew H. Raedeke, Rudy M. Schuster, Mark P. Vrtiska

School of Natural Resources: Faculty Publications

Canadian and American waterfowl hunters were surveyed to identify their hunting trip preferences. Respondents were individuals that were now participating or had participated in waterfowl hunting, and most had hunted the majority of the last five years. We identified four latent classes of waterfowl hunters that varied in their preferences for harvest, access effort, length of travel, quantity of waterfowl seen, and the potential for interference/competition. We found a diminishing return associated with the number of waterfowl harvested, and that ‘devoted’ and ‘local’ hunters did not perceive appreciable benefit from harvesting more birds beyond harvesting a single bird. Results highlight …

Mitosis Mystery!, Lluvia Garcia

Critical Proceedings of the STEM ED Conference (2023)

No abstract provided.

The Rider (2024-02-26), Natalie Lapsley

The Rider Archives

Vol.9 No.21

Caregivers' Attachment Relationships With Infants And Toddlers During The Covid-19 Pandemic, Rebecca Ellen Roth

Walden Dissertations and Doctoral Studies

Health and safety protocols related to the COVID-19 pandemic and its variants continue to influence practices in infant–toddler programs one western state in the United States; caregivers in the state are concerned about attachment relationships with infants and toddlers. The purpose of this basic qualitative study was to explore caregiver perceptions of attachment relationships related to the COVID-19 pandemic and its variants and the influence of health and safety protocols on caregiver practices. The conceptual framework combined attachment and biobehavioral theories. Data from interviews with four infant and six toddler caregivers were recorded, transcribed, and analyzed by searching for patterns …

Ecology And Management Of The Crapemyrtle Aphid (Hemiptera: Aphididae) On Crapemyrtle (Myrtales: Lythraceae) In The Southern United States, Caleb J. Wilson, Matthew A. Bertone

Entomology Faculty Publications

Crapemyrtle aphid [Sarucallis kahawaluokalani (Kirkaldy 1907)] (CMA) is an introduced pest of crapemyrtle (Lagerstroemia spp. L.), which is among the most common ornamental tree genera in the southern United States. Native to Southeast Asia, CMA has become established on 5 other continents. CMAs produce honeydew as they feed which supports sooty mold growth and can result in leaf defoliation on infested trees. CMAs tend to be most abundant in crapemyrtles planted in locations with extensive surrounding impervious surface cover. A large community of generalist natural enemies are found in close association with CMA, which suggests that CMAs may help conserve …

The Mirror Project: Reflections On The Experiences Of African-American Female Adolescents Experiencing Foster Care, Bahia Anise-Cross Degruy Overton

Dissertations and Theses

As the author Zora Neale Hurston says, "If you're silent about your pain, they'll kill you and say you enjoyed it." The Mirror Project (MP) aims to break this silence by giving voice to Black women who have experienced foster care in Portland, Oregon during their adolescence. In focus groups and interviews, participants shared their stories. Racial identity development theory, phenomenology and Afrocentric feminist epistemology provided lenses for gaining insight into their experiences in a predominantly white city. The MP revealed six themes: lack of youth engagement in foster care decisions, the need for a cultural lens in social work, …

Exploring The Mechanical Strength, Antimicrobial Performance, And Bioactivity Of 3d-Printed Silicon Nitride-Peek Composites In Cervical Spinal Cages, Cemile Basgul, Paul Desantis, Tabitha Derr, Noreen J. Hickok, Ryan M. Bock, Steven M. Kurtz

Department of Orthopaedic Surgery Faculty Papers

In this study, our goal was to assess the suitability of a polyether-ether-ketone (PEEK) and silicon nitride (Si3N4) polymer composite for antimicrobial three-dimensional (3D)-printed cervical cages. Generic cage designs (PEEK and 15 vol.% Si3N4-PEEK) were 3D-printed, including solid and porous cage designs. Cages were tested in static compression, compression shear, and torsion per ASTM F2077. For antibacterial testing, virgin and composite filament samples were inoculated with Staphylococcus epidermidis and Escherichia coli. In vitro cell testing was conducted using MC3T3-E1 mouse preosteoblasts, where cell proliferation, cumulative mineralization, and osteogenic activity were measured. The 3D-printed PEEK and Si3N4-PEEK cages exhibited adequate mechanical …

Scgist: Gene Panel Design For Spatial Transcriptomics With Prioritized Gene Sets, Mashrur Ahmed Yafi, Md Hasibul Husain Hisham, Francisco Grisanti, James F Martin, Atif Rahman, Md Abul Hassan Samee

Faculty, Staff and Students Publications

A critical challenge of single-cell spatial transcriptomics (sc-ST) technologies is their panel size. Being based on fluorescence in situ hybridization, they are typically limited to panels of about a thousand genes. This constrains researchers to build panels from only the marker genes of different cell types and forgo other genes of interest, e.g., genes encoding ligand-receptor complexes or those in specific pathways. We propose scGIST, a constrained feature selection tool that designs sc-ST panels prioritizing user-specified genes without compromising cell type detection accuracy. We demonstrate scGIST's efficacy in diverse use cases, highlighting it as a valuable addition to sc-ST's algorithmic …