Digital Commons Network^™

Ongoing Study Of The Association Of Apoe Gene Polymorphisms With Cognitive Impairment In The Rio Grande Valley Hispanic Population, Jaime Miguel L. Saveron, Daniela Gamez, Chun Xu

Research Symposium

Background: Alzheimer's disease (AD), a prevalent neurodegenerative disorder, exhibits a significant genetic component. In addition, mild cognitive impairment (MCI) is a key risk factor for AD. While certain demographic factors and genetic variants associated with AD and cognitive impairment have been identified in non-Hispanic populations, limited research exists on this subject within the Hispanic population, particularly in the Rio Grande Valley. This study aims to investigate the relationship between the APOE gene, specifically its ε4 allele, and cognitive impairment in the Hispanic population of the Rio Grande Valley.

Methods: A total of 269 Hispanic subjects, aged 60 and above, with …

Newly Diagnosed Ulcerative Colitis In A Young Hispanic Mexican Female At South Texas: A Case Report, Elimar Gonzalez Morales, Jose Gomez Casanovas, Shreel Patel, Khairiya Haj-Yahya

Research Symposium

Introduction: Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) characterized by recurring episodes of inflammation limited to the mucosal layer of the colon. Traditionally, this disease has been associated mostly with white Americans, however in recent years, an increase incidence has been reported in minorities in the US and around the world. In Hispanic population, cases are frequently underdiagnosed, and literature is scarse. In this case, we present a young patient without past medical history who presented with what appeared to be an infectious gastroenteritis and found to have a severe UC.

Case Presentation: A 22-year old Hispanic …

Nebulization Based Inhalation Nanomedicine For Lung Cancer Treatments, Rahul Tiwari, Meghana Kolli, Neeraj Chauhan, Eswara Naga Hanuma, Vivek Kashyap, Subhash Chauhan, Murali Yallapu

Research Symposium

Background: Lung cancer is reported to have a high incidence rate and first leading cause of cancer-related morbidity and mortality across the world including in the United States. Noninvasive nebulized inhalation is a promising delivery strategy for lung, which can enhance the targeting efficiency and detention time interval of nanoparticles in the lung tissue, thus elevating the therapeutic index of therapeutic agent(s) at lower dosages. The aim of this study is to develop inhalable nanoparticles (INPs) for effective delivery of therapeutic agents in lung cancer cell lines and ex vivo models.

Methods: The inhalation nanoparticles (INPs) were prepared by solvent …

Modes Of Fracture Of Flat Bone: Fracture Direction And Stress, Nolan Wang, Mary K. Holden, Everett Baker, Nathaniel Lerch, Nate Fitch

Seaver College Research And Scholarly Achievement Symposium

This study delineates the application of fractography to discern the multifaceted modes of fracture in flat bones, explicating the interplay between fracture directionality and stress states regarding the viscoelastic nature of bone. Despite the perceived brittleness, bone exhibits toughness, attributed to its intricate structure design and amalgamating properties of solid and viscous materials. This research elucidates the variegated fracture modes inherent to bone's dualistic nature by examining fracture surfaces and identifying fracture markings using stereoscopic microscopy. Our observations across seven bone specimens reveal predominant fracture patterns from top to bottom in six instances, with a singular deviation where the fracture …

Coomassie Brilliant Blue Dye As A Method For Analyzing Fracture Markings In Bone, Abigail Hoffmeister, David Harutunyan, Matthew Aizawa, Everett Baker, Brandon Mendoza, Chase Freeman, Siran Iskanian

Seaver College Research And Scholarly Achievement Symposium

Coomassie Brilliant Blue Dye is a dye commonly used to stain proteins. Because of its ability to adhere to proteins, this research has focused on perfecting a method of dyeing a fractured flat bone in order to most accurately observe and analyze fracture markings within the trabecular layer. Stereoscopic microscopy was the chosen technique of analysis for this research because of its proven effectiveness in glass and ceramic fractography to observe varying depths. In order to most effectively apply stereoscopic microscopy to this research, the following variables were manipulated to maximize color contrast in the trabecular layer in order to …

Network Evaluation Of Influential Sensors: A Proposed Approach, John Mello, Kamryn Kamps

Seaver College Research And Scholarly Achievement Symposium

In 2013, the White House issued Presidential Policy Directive 21 (PPD-21) on Critical Infrastructure Security and Resilience to advance a nationally unified effort to ensure a secure, functioning, and resilient critical infrastructure. In 2017, US Senator Dianne Feinstein expressed her concern that inspections conducted on critical infrastructure may not adequately consider the safety consequences of increasingly extreme weather patterns linked to climate change or considerable seismic hazards. Engineers responded to these opportunities by updating surveillance and monitoring programs designed to capture actual infrastructure behavior during operations. While advances in modern instrumentation and computer technologies make it easier to acquire behavior …

Mathematically Modeling How Trapping Regimes That Target Specific Crayfish Life Stages Impact Removal Efficacy, Rini Pattison

Seaver College Research And Scholarly Achievement Symposium

The red swamp crayfish, Procambarus clarkii, is an invasive species introduced into several streams within the Santa Monica Mountains (SMM) in Southern California. Crayfish predation decimates native aquatic species. Thus, the Mountains Restoration Trust (MRT) and Environmental Restoration Group have worked to remove crayfish through regular trapping in Malibu Creek.

To aid conservation efforts, former SURB students William Milligan and Dev Patel developed mathematical models of crayfish removal efficacy. Milligan created a differential equation model of how crayfish removal affects local newt populations. Patel expanded Milligan’s crayfish model by creating a discrete model of the crayfish life cycle that newly …

Drone Imagery Of Submarine Groundwater Discharge Effects On Coastal Primary Productivity On Coral Reefs In Maunalua Bay, Hawai'i, Lulu Wang, Florybeth Flores La Valle, Grace Kim

Seaver College Research And Scholarly Achievement Symposium

Submarine groundwater discharge (SGD) plays a crucial role in coastal ecosystems, especially coral reefs, by introducing terrestrial nutrients and freshwater, significantly affecting the primary productivity of coastal ecosystems and potentially altering ecological balances. La Valle et al. (2023) emphasized the importance of SGD in nutrient distribution and algal productivity, highlighting the need for comprehensive understanding and management strategies in the face of increasing nutrient runoff due to land use changes due to increased population. To better understand the impact, our experiments utilize remote sensing and data science by using a DJI Mavic 3 Enterprise drone with a thermal imaging camera …

Deep Learning Can Be Used To Classify And Segment Plant Cell Types In Xylem Tissue, Reem Al Dabagh, Benjamin Shin, Sean Wu, Fabien Scalzo, Helen Holmlund, Jessica Lee, Chris Ghim, Samuel Fitzgerald, Marinna Grijalva

Seaver College Research And Scholarly Achievement Symposium

Studies of plant anatomical traits are essential for understanding plant physiological adaptations to stressful environments. For example, shrubs in the chaparral ecosystem of southern California have adapted various xylem anatomical traits that help them survive drought and freezing. Previous studies have shown that xylem conduits with a narrow diameter allows certain chaparral shrub species to survive temperatures as low as -12 C. Other studies have shown that increased cell wall thickness of fibers surrounding xylem vessels improves resistance to water stress-induced embolism formation. Historically, these studies on xylem anatomical traits have relied on hand measurements of cells in light micrographs, …

Checking For Ticks: Analyzing The Efficiency Of Different Tick Collection Methods, Peter Briggs, Lawson Trimmell, Javier Monzón

Seaver College Research And Scholarly Achievement Symposium

Ticks are the most important arthropod disease vectors in both North America and Europe. Field collection of wild ticks is vital for research on the ecology of vector-borne diseases. Dragging and trapping are the two most common methods for collecting wild ticks. Dragging involves a researcher pulling a canvas through a field to collect ticks searching for a host, while trapping exploits ticks’ natural attraction to carbon dioxide to lure them onto a canvas where they get caught on tape. Our study aimed to evaluate which of these two methods is more effective. We chose six sites across three states, …

The Effect Of Submarine Groundwater Discharge On Greenhouse Gas Production On A Coral Reef In Maunalua Bay, Hi, Kylie Nichols

Seaver College Research And Scholarly Achievement Symposium

THE EFFECT OF SUBMARINE GROUNDWATER DISCHARGE ON GREENHOUSE GAS EMISSIONS IN MAUNALUA BAY, HI

Authors: Kylie Nichols, Dr. Carmella Vizza, Dr. Florybeth La Valle

Abstract: In the urban coastal environments of Honolulu, Hawai’i, water high in inorganic nutrients such as nitrates, phosphates, and ammonia, often becomes further enriched by human activity. This water seeps into the ocean by mode of submarine groundwater discharge (SGD).

We hypothesize that SGD influences greenhouse gas (GHG) production in coastal systems at a measurable rate and is overlooked as a significant source of methane emissions. Marshes and wetlands are the largest contributors to methane production …

Effectiveness Of Frog Skin Secretions Against Uv-B Radiation, Connor Mcgaha, Kinkade Mcmurray, Lee B. Kats

Seaver College Research And Scholarly Achievement Symposium

As an increasing number of their species face possible extinction, amphibian populations have been steadily declining over the past decades (IUCN). While various factors have been suggested or proven to be partially responsible for this reduction, increased ultraviolet B radiation (UVBR) appears to be one of the more prominent explanations, especially when considering the continual thinning of the Earth’s ozone layer due to anthropogenic climate change. However, it has also been discovered that skin secretions, which are reported to function as a component of amphibians’ innate immune systems, may have an effect on UVBR (Cramp and Franklin). With this in …

A Tick's Quest: The Effects Of Climatic Stress On Host-Seeking Behavior Of Wild Ticks, Lawson Trimmell, Peter Briggs, Javier Monzón

Seaver College Research And Scholarly Achievement Symposium

The Lone Star Tick (Amblyomma americanum) is one of the most epidemiologically relevant tick species in the United States and is experiencing a range expansion. Our study aimed to investigate the effects of climatic stress on the behavior and physiology of the Lone Star Tick at the expansion front. We hypothesized that there are differences in survivorship, questing (host-seeking) behavior, and dehydration tolerance based on sex or geographic origin. We also predicted that ticks increase their host-seeking behavior as they approach death due to dehydration stress. We collected adult ticks from six sites across Oklahoma and New York/New Jersey. We …

Effect Of Three-Month Use Of Assistive Shoes On Gait In Patients With Peripheral Artery Disease, Jonathan Kirkland

UNO Student Research and Creative Activity Fair

Effect of Three-Month Use of Assistive Shoes on Gait in Patients with Peripheral Artery Disease

Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., Christian Wintle

UNO Student Research and Creative Activity Fair

Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).

Christian J. Wintle^a, Jordan B. Hernandez^a,b,c, Dobromir Dotov^d, and Jonathan B. Clayton^a,b,e,f,g

^aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA

^bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA

^cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA

^dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA

^eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, …

Mechanistic Investigation Of C—C Bond Activation Of Phosphaalkynes With Pt(0) Complexes, Roberto M. Escobar, Abdurrahman C. Ateşin, Christian Müller, William D. Jones, Tülay Ateşin

Research Symposium

Carbon–carbon (C–C) bond activation has gained increased attention as a direct method for the synthesis of pharmaceuticals. Due to the thermodynamic stability and kinetic inaccessibility of the C–C bonds, however, activation of C–C bonds by homogeneous transition-metal catalysts under mild homogeneous conditions is still a challenge. Most of the systems in which the activation occurs either have aromatization or relief of ring strain as the primary driving force. The activation of unstrained C–C bonds of phosphaalkynes does not have this advantage. This study employs Density Functional Theory (DFT) calculations to elucidate Pt(0)-mediated C–CP bond activation mechanisms in phosphaalkynes. Investigating the …

Lncrna Impact On Regorafenib Resistance In Colorectal Cancer, Ricardo Pequeno Bracho, Kyle Doxtater, Dennis Kwabiah, Yamile Abuchard Anaya, Sophia Leslie, Mohammad Shabir Hussain, Manish Tripathi

Research Symposium

Cancer metastasis is one of the deadliest aspects of the disease, with about 90% of all cancer-related deaths due to its development at different sites within the body. Colorectal cancer (CRC) is the second leading cause of cancer mortality in the United States, with 40-50% of all patients developing metastasis at some point during their fight with the disease. With the approval of Regorafenib for treating metastatic colorectal cancer, steps have been taken to combat metastasis in colorectal cancer. A vital aspect of the development of metastasis is the development of resistance to first-line chemotherapy. Regorafenib is an oral small-molecule …

La1-Xsrxcoo3 Perovskite Nanomaterial: Synthesis, Characterization, And Its Biomedical Application, Adhira Tippur, Anyet Shohag, Luke Franco, Ahmed Touhami, Swati Mohan, Mohammed Uddin

Research Symposium

Early cancer detection is paramount for effective treatment and potential cures. This research explores the application of perovskite materials, specifically Sr2+-doped Lanthanum Cobaltite (La1-xSrxCoO3) nanomaterials, in cancer detection, with a focus on rats as an experimental model. The ferroelectric nature of these materials, synthesized through a combination of sol-gel and molten-salt processes, was examined at varying Sr2+ doping levels (1-20 wt%). Rigorous characterization, employing X-ray diffraction and scanning electron microscopy, confirmed the uniform morphology of nano cubes, laying the foundation for subsequent investigations. The magnetic properties of the perovskite nanoparticles were probed, suggesting their potential as a diagnostic tool for …

Validation And Implementation Of Human-Specific Multiplex Analysis To Quantify The Inflammatory Response In The Plasma Of Common Marmosets (Callithrix Jacchus), Donald Rogers

UNO Student Research and Creative Activity Fair

Validation and Implementation of Human-specific Multiplex Analysis to Quantify the Inflammatory Response in the Plasma of Common Marmosets (Callithrix jacchus)

Donald R. Rogers¹, Aliyah Jabenis^1,2, Mackenzie Conrin, M.S.^1,3, Shivdeep Hayer, D.V.M, Ph.D.¹, Claudia Rauter Ph.D.¹, Noel Johnson, D.V.M., M.P.H.⁴, Jonathan B. Clayton, D.V.M., Ph.D.^1,5,6,7, Paul W. Denton, Ph.D.^1,7

¹University of Nebraska at Omaha, Department of Biology

²University of Nebraska Medical Center, Department of Neuroscience

³University of Nebraska Medical Center, Environmental Health and Safety

⁴University of Nebraska Medical Center, Department …

Influence Of Neurotoxin Load On Parkinson’S Disease Pathophysiology, Abhishekh Pokhrel, Daniel Salinas, Nawaz Hack, Kelsey Potter-Baker

Research Symposium

Background: Parkinson’s disease (PD) is a debilitating neurodegenerative disorder impacting movement, mood, and cognition. Among those affected, veterans, due to their occupational exposures, are particularly susceptible, contributing to over 110,000 PD cases in the United States. Studies have largely attributed this increased prevalence among veterans to environmental neurotoxins such as Agent Orange, MPTP, and 6-OHDA. However, it remains unclear how neurotoxin exposure load influences biological mechanisms in PD. This study aims to elucidate the influence of neurotoxin load on PD-associated molecular changes, neuroplasticity, neurodegeneration, and cognitive and motor function within a clinical population of the Rio Grande Valley region …

Human Blood Cell Isolation: The Critical First Step In Our Laboratory’S Immunobiology Experimental Protocals, Victor Rivero

UNO Student Research and Creative Activity Fair

HUMAN BLOOD CELL ISOLATION: THE CRITICAL FIRST STEP IN OUR LABORATORY’S IMMUNOBIOLOGY EXPERIMENTAL PROTOCALS

Victor Rivero1 Paul W. Denton1, [email protected]

1Department of Biology, University of Nebraska at Omaha, Omaha, NE

The Denton Immunobiology Laboratory focuses on enhancing human natural killer (NK) cell killing capabilities, particularly in the context of combating cancer. NK cells are immune cells that have the ability to kill diseased cells via two mechanisms: direct killing, and antibody-dependent cell-mediated cytotoxicity (ADCC). We recently published our novel approach to testing both methods of killing by using NK cells derived from the same human donor. Our testing approach allows …

Highlights Of The 2023 Brano Heart Failure Forum, Bela Merkely, Rajko Radovancevic, Bojan Vrtovec, Igor D. Gregoric

The VAD Journal

Since 2007, the Branislav “Brano” Radovancevic Heart Failure Forum (BHFF) has been held annually to provide a venue for experts to present and discuss “Innovations and New Treatment Strategies in Heart Failure.” Clinicians and researchers gather yearly in a different Eastern European city to discuss the latest in heart failure diagnostics and therapeutics. The 2023 BHHF forum was held on the 13^th thru 15^th of September 2023 in Budapest, Hungary. It was attended by over 100 faculty from 16 countries. In addition, participation through online streaming was available. Throughout the forum, 16 sessions focused on challenges and solutions …

Honey Targets Ribosome Biogenesis Process In Human Pancreatic Cancer Cells To Inhibit Their Growth And Metastatic Phenotypes, Aun A. Bangash, Muhammad Bangash, Haider Ahsan, Shiza Khan, Mudassier Ahmad, Dae Joon Kim, Sahir Alvi, Bilal Hafeez

Research Symposium

Background: Pancreatic cancer (PanCa) is the fourth deadliest cancer worldwide and is expected to become the second deadliest cancer by 2030. In the USA, the National Cancer Institute put forth a grim prediction stating that there will be 64,050 new cases in 2023 alone and about 50,000 of these patients will die. Existing therapeutic regimens against PanCa are not that effective and show unacceptable toxicities. Therefore, developing highly effective new agents with less toxicity is urgently required, which could be used as a monotherapy or as an adjuvant to treat PanCa patients. Honey is known for its tremendous health benefits …

The Benefits Of Peer Visitation On The Rehabilitation Of New Amputees: A Qualitative Study, Bruce N. Elliott, Caitlyn Ofiesh, Dpt, Shannon Morrisey Dpt, Julie Mulcahy Dpt, Kristina Economou Dpt, Dennis Beaudry Dpt

Internet Journal of Allied Health Sciences and Practice

Purpose: For the purpose of this study a peer visitor is being defined as someone who has experienced some type of limb loss and is now mentoring other amputees. Their focus is on health and recovery as opposed to illness as they listen, provide emotional support, and most importantly inspire hope in an individual who has experienced a new amputation. The primary purpose of this study is to examine the reports of amputees regarding their personal experiences related to whether they spoke with a peer visitor following surgery. The study focuses on the benefits of peer visitation and how it …

Pilot Study For Use Of An Arthrometer To Assess Joint Laxity In People With Ehlers-Danlos Syndrome, Dawn T. Gulick, Valerie Iovine, Kerstin Palombaro

Internet Journal of Allied Health Sciences and Practice

Purpose: Joint hypermobility is a condition in which synovial joints move beyond normal limits. Approximately 10-25% of children and 5-25% of adults experience hypermobility syndrome. One such hypermobility syndrome is an inherited connective tissue disorder known as Ehlers-Danlos Syndrome (EDS). Typically, a score of 4-5 out of 9 on the Beighton scale is indicative of hypermobility in adults. Whereas 6 out of 9 is the criteria for children. No significant correlations were found between the systemic features of EDS and the Beighton score. The purpose of this pilot study was to see if an arthrometer could be used …

Experiences Of Minoritized Learners In Preparation For Graduate Medical And Health Care Education, Ashley M. White, Lindsey E. Eberman, Matthew J. Drescher, Justin Young, Kenneth E. Games

Internet Journal of Allied Health Sciences and Practice

Context: Disparities in the healthcare system persist in society today, affecting both minoritized patients and providers. A diverse healthcare workforce is ideal to treat a patient population that is also becoming increasingly diverse. We examined the experiences of minoritized students pursuing healthcare-related degrees, including athletic training, in pre-medicine and healthcare professions. Methods: We used a phenomenological approach to explore the lived experiences of 10 minoritized pre-medicine and healthcare profession students (age=20±2 years). Participants engaged in an online semi-structured interview (Zoom, San Jose, CA). All interviews were audio-recorded and transcribed. We used a consensual qualitative research (CQR) approach with a 3-person …

Educational Lessons From A Pandemic: Lecture Recording And Learning Outcomes, Melissa Lesser, Lora Packel

Internet Journal of Allied Health Sciences and Practice

Purpose: The use of lecture capture (LC) in higher education classrooms has increased, especially since the onset of the COVID-19 pandemic; however, its effectiveness as a learning strategy is largely untested in graduate students. In undergraduate students, the use of LC has mixed findings related to academic performance. Some studies show no improvement in assessment or course grades while others show small gains. The purpose of this study was to investigate the impact of LC on academic performance in graduate doctor of physical therapy students (DPT). Methods: In module 1, students received two virtual synchronous lectures which were recorded …

Student Perspectives On Transition From Bachelor’S To Master’S Degree For Professional Athletic Trainers: Advantages And Disadvantages, Bobbi A. Severt, Michael B. Phillips, Janet Kesterson Isbell

Internet Journal of Allied Health Sciences and Practice

Purpose: The purpose of this study is to understand the perspectives of students interested in a career in athletic training on the degree change and what they perceive as advantages and disadvantages to pursuing a master's level professional degree in athletic training. Method: A case study was conducted using an interpretivism framework informed by a constructionism theoretical stance. A focus group of six current undergraduate pre-athletic training students and individual interviews of three pre-athletic training graduates along with current and historical documents were used to collect data. An inductive approach was used to analyze data along with reflexive thematic analysis …

Molecular Mechanism Regulating The Fate And Function Of The Drosophila Sleep-Wake Circuit, Ethan Cato Wilson, Mubarak Hussain Syed

Brain & Behavioral Health Research Day

Post-bachelor research showing the evaluation of IGF2 mRNA-binding protein expression in adult Drosophila type II neural stem cells in the development of the sleep-wake circuit within the dorsal fan shape body. The genetic silencing of Imp on Chromosome III is sufficient to reduce the amount of sleep-wake neurons expression.

Funding: This research was supported by the National Science Foundation, the Sloan Foundation, and the Brain & Behavior Research Foundation

Within-Field Soil Moisture Variability And Time-Invariant Spatial Structures Of Agricultural Fields In The Us Midwest, Yi Yang, Bin Peng, Kaiyu Guan, Ming Pan, Trenton E. Franz, Michael H. Cosh, Carl J. Bernacchi

School of Natural Resources: Faculty Publications

Understanding soil moisture variability and estimating high-resolution soil moisture at subfield to field scales is critical for agricultural research and applications. However, systematic investigation of subfield scale soil moisture variability over cropland is still lacking from both measurement and satellite remote sensing. In this study, we aim to investigate (1) the characteristics of within-field soilmoisture distribution over typical cropland in the US Midwest and (2) the capabilities of satellite remote sensing in capturing the spatiotemporal variabilities of soil moisture at subfield scale. Specifically, we conducted soil moisture field experiments in three typical commercial agricultural fields (∼85 acres per field) in …