Digital Commons Network^™

How Monitoring Food Is Protecting People, Grace Sugden

Capstone Projects and Master's Theses

While the issue of food safety has always been a concern for humanity, what that looks like has changed drastically over time. Instead of trying to judge if a stream has safe drinking water, consumers are now faced with deciding which preservatives and pesticides they expose themselves to. With the multitude of United States agencies tasked with protecting the public, standards and quality control processes should be transparent and efficient. Since the creation of the Bureau of Chemistry, numerous acts have been passed that regulate ingredients, processing procedures, and minimize contamination risks. When a spoiled product enters the food web, …

Parenting A Child Who Has An Autistic Sibling: Effects On Adjustment And Mental Health, Jess Del Mundo

Capstone Projects and Master's Theses

This capstone project focuses on informing parents about the challenges and needs of siblings of children with autism. Parenting is a profound and meaningful responsibility, and having children with special needs adds to this daunting job. It is imperative for parents to nurture the development and awareness of all of their children, both neurodivergent and neurotypical. This workshop will give parents the tools to navigate this responsibility. I developed a workshop that first informs parents about the positive outcomes for children who have siblings with autism, such as higher levels of empathy and compassion. Second, I will address the challenges …

Sonidos De Aztlán: A Historical Analysis Of Chicano Music, Alejandro Gomez

Capstone Projects and Master's Theses

This paper analyzes music made primarily by Chicanos in the U.S. and social movements that the music was a part of. Case studies include the Zoot Suit Riots, the Delano Grape Strike, The Chicano Movement, Tejano/Conjunto and Tex-Mex, Narcocorridos, and the Chicanx Renaissance.

The Impact Of Parental Involvement On Academic Achievement And Self-Concept Of Elementary School Students, Alexis Randall

Capstone Projects and Master's Theses

Lack of parental involvement in schools is the missing link to long-term student success at the elementary school level. Within the first few years of elementary school, parental help and support is all that students need as they begin to grow and learn new concepts at school The continuing absence of parental involvement in school can have a long-term impact on the students, hindering their growth in the years to come in their academics and self-concept. A literature review and surveys with parents and teachers indicated that the lack of parental involvement in schools contributed to students’ behaviors, lack of …

Whispers Of Thyme: Echoes Of Herbal Wisdom, Gianna Constantine

Capstone Projects and Master's Theses

Traditional herbal medicines used for centuries are now highly uncommon compared to the man made pharmaceuticals doctors have prescribed us with. Yes, they help and do what they are meant to but how come we go to these first? Why instead of peppermint or ginger tea, do we take pepto bismol? Without a thought we immediately turn to the chemically man made medicines marketed to us rather than trying something natural that has the same purpose first. We pollute the Earth that has done nothing but given us the tools to help ourselves and our discomforts, while relying on our …

Implementing An Enhanced Care Management Program Serving Post-Incarcerated Individuals, Brianna Tome

Capstone Projects and Master's Theses

The post-incarcerated population has poor health and social outcomes in San Benito and Santa Clara counties. Youth Recovery Connections is a nonprofit organization serving individuals with substance use disorders, gang, and justice involved individuals. The Enhanced Care Management program was implemented in the organization with the addition of serving post-incarcerated individuals. Contributing factors that lead to negative health outcomes include lack of case managers with lived experience, lack of self management skills, and instability of care transitions. Consequences that can arise from these factors include increased healthcare costs, recidivism, and lack of stable housing. The identified consequences cause negative health …

Nuestro Futuro: Exploring Perspectives About Cultivating Bilingual & Bicultural Latinx Educators In California, Jaime Garcia Ramirez

Capstone Projects and Master's Theses

In response to the nationwide effort to cultivate, recruit, and retain qualified bilingual and bicultural Latinx teachers and the growing demand for bilingual education in California, this qualitative study explores the perceptions of teacher educators at University X. The research considers the significant presence of emergent bilingual learners (EBLs), the increasing Latinx population, teacher shortages, and high attrition rates. The study aims to investigate how University X educators and teachers perceive initiatives designed to increase the number of bilingual and bicultural Latinx educators and their suggestions for improvement. Through a comprehensive analysis of collected data and an extensive review of …

Increasing Preschool Children’S Scientific Inquiry, Dianna Leon

Capstone Projects and Master's Theses

This capstone project focused on increasing scientific inquiry in young children. The problem that exists is that there is not enough focus on scientific learning in preschool settings compared to school settings with older age groups. According to Dejarnette (2018), young children can learn about science and are naturally curious, making them natural scientists. Science curriculum has many benefits for children, such as the development of observation, documentation, investigation, communication, and literacy skills. Science curriculum can create curiosity and enjoyment in young children while they learn about the world around them, creating a lifelong interest in learning (Larimore, 2020). Exciting …

Addressing Food Insecurity Among College Students, Serena Plares

Capstone Projects and Master's Theses

In this capstone project, the focal topic was food insecurity and food assistance with the target population being college students attending California State University Monterey Bay. This demographic includes a diverse group of young adults from various socioeconomic backgrounds and cultures. Food insecurity is rampant across our country, county, and college campuses. It impacts families and individuals of all backgrounds and there is a need for knowledge pertaining to this topic and affordable, nutritious food access and resources. One in three children and one in four adults are affected by food scarcity in Monterey County and thirty-four percent of county …

Imposter Syndrome: Navigating As A First-Generation College Student, Jessica Hamm

Capstone Projects and Master's Theses

The focal topic of this capstone project is navigating imposter syndrome among first-generation college students. Imposter syndrome is an internal experience in which you believe you are less capable than others judge you to be. It is a mental state in which you doubt your own talents/abilities. In a community jeopardized by an influx of what is called imposter syndrome, there exist students who are high achieving yet fail to recognize their success as being earned or deserved. Imposter syndrome describes an individual who is high achieving yet fails to recognize their success as being earned, but instead attributes it …

Mental Health Awareness In Adolescents And The Impact Of Social Media, Vanessa Clark

Capstone Projects and Master's Theses

This capstone project focuses on informing parents of the effects of social media on adolescents’ mental health. Adolescents are frequent users of social media as they are more likely to explore online content and be unaware of the potential dangers that can pose a threat to their mental health (Rapee et al. 2019). Due to social media being difficult to monitor at all times, along with advances in technology through school curriculums, we want to acknowledge its presence and usage. Social media allows the opportunity to connect with others digitally but there needs to be awareness of the potentially harmful …

The Viral Spread Of Xenophobia: Analyzing The Influence Of Far-Right Politics On Anti-Asian Sentiment Expressed On Social Media During Covid-19., Robert F. Arnold Iii

Capstone Projects and Master's Theses

Throughout history, viral outbreaks have frequently led to the emergence of biased and discriminatory attitudes, often targeting populations that deviate from Western norms. These sentiments commonly manifest as xenophobia and racism (Chen, 2020; Chou, 2021). This report serves as an in-depth analysis of the role far-right political phrasing of Covid-19 as the “Chinese Virus,” played in the proliferation of anti-Asian and xenophobic attitudes, expressed on the social media platform X (Twitter). This study also aims to better understand the various different factors that cause followers of far-right politics to engage and believe in anti-Asian sentiment online. Using Integrated Threat Theory …

The Constitutively Active Form Of A Key Cholesterol Synthesis Enzyme Is Lipid Droplet-Localized And Upregulated In Endometrial Cancer Tissues, Hudson W Coates, Tina B Nguyen, Ximing Du, Ellen M Olzomer, Rhonda Farrell, Frances L Byrne, Hongyuan Yang, Andrew J Brown

Faculty, Staff and Student Publications

Cholesterol is essential for both normal cell viability and cancer cell proliferation. Aberrant activity of squalene monooxygenase (SM, also known as squalene epoxidase), the rate-limiting enzyme of the committed cholesterol synthesis pathway, is accordingly implicated in a growing list of cancers. We previously reported that hypoxia triggers the truncation of SM to a constitutively active form, thus preserving sterol synthesis during oxygen shortfalls. Here, we show SM truncation is upregulated and correlates with the magnitude of hypoxia in endometrial cancer tissues, supporting the in vivo relevance of our earlier work. To further investigate the pathophysiological consequences of SM truncation, we …

Profiling The Activity Of The Para-Caspase Malt1 In B-Cell Acute Lymphoblastic Leukemia For Potential Targeted Therapeutic Application, Firas M Safa, Terri Rasmussen, Lorena Fontan, Min Xia, Ari Melnick, Adrian Wiestner, Patricia Lobelle-Rich, Jan A Burger, Yara Mouawad, Hana Safah, Erik K Flemington, Nakhle S Saba

Faculty, Staff and Student Publications

B-cell acute lymphoblastic leukemia (B-ALL) remains a hard-to-treat disease with a poor prognosis in adults. Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is a para-caspase required for B-cell receptor (BCR)-mediated NF-κB activation. Inhibition of MALT1 in preclinical models has proven efficacious in many B-cell malignancies including chronic lymphocytic leukemia, mantle cell lymphoma and diffuse large B-cell lymphoma. We sought to examine the role of MALT1 in B-ALL and determine the biological consequences of its inhibition. Targeting MALT1 with both Z-VRPR-fmk and MI-2 efficiently kills B-ALL cells independent of the cell-of-origin (pro, pre, mature) or the presence of the Philadelphia …

Dlk-Mapk Signaling Coupled With Dna Damage Promotes Intrinsic Neurotoxicity Associated With Non-Mutated Tau, Sanming Li, Ethan R Roy, Yanyu Wang, Trent Watkins, Wei Cao

Faculty, Staff and Student Publications

Alzheimer's disease (AD) is the most prevalent form of neurodegeneration. Despite the well-established link between tau aggregation and clinical progression, the major pathways driven by this protein to intrinsically damage neurons are incompletely understood. To model AD-relevant neurodegeneration driven by tau, we overexpressed non-mutated human tau in primary mouse neurons and observed substantial axonal degeneration and cell death, a process accompanied by activated caspase 3. Mechanistically, we detected deformation of the nuclear envelope and increased DNA damage response in tau-expressing neurons. Gene profiling analysis further revealed significant alterations in the mitogen-activated protein kinase (MAPK) pathway; moreover, inhibitors of dual leucine …

The Intrinsic Substrate Specificity Of The Human Tyrosine Kinome, Tomer M Yaron-Barir, Brian A Joughin, Emily M Huntsman, Alexander Kerelsky, Daniel M Cizin, Benjamin M Cohen, Amit Regev, Junho Song, Neil Vasan, Ting-Yu Lin, Jose M Orozco, Christina Schoenherr, Cari Sagum, Mark T Bedford, R Max Wynn, Shih-Chia Tso, David T Chuang, Lei Li, Shawn S-C Li, Pau Creixell, Konstantin Krismer, Mina Takegami, Harin Lee, Bin Zhang, Jingyi Lu, Ian Cossentino, Sean D Landry, Mohamed Uduman, John Blenis, Olivier Elemento, Margaret C Frame, Peter V Hornbeck, Lewis C Cantley, Benjamin E Turk, Michael B Yaffe, Jared L Johnson

Faculty, Staff and Student Publications

Phosphorylation of proteins on tyrosine (Tyr) residues evolved in metazoan organisms as a mechanism of coordinating tissue growth1. Multicellular eukaryotes typically have more than 50 distinct protein Tyr kinases that catalyse the phosphorylation of thousands of Tyr residues throughout the proteome1-3. How a given Tyr kinase can phosphorylate a specific subset of proteins at unique Tyr sites is only partially understood4-7. Here we used combinatorial peptide arrays to profile the substrate sequence specificity of all human Tyr kinases. Globally, the Tyr kinases demonstrate considerable diversity in optimal patterns of residues surrounding the site of phosphorylation, revealing the functional organization of …

Bacteria Synergized With Pd-1 Blockade Enhance Positive Feedback Loop Of Cancer Cells-M1 Macrophages-T Cells In Glioma, Qi Chen, Yuyi Zheng, Xiaojie Chen, Yuan Xing, Jiajie Zhang, Xinyi Yan, Qi Zhang, Di Wu, Zhong Chen

Faculty, Staff and Student Publications

Cancer immunotherapy is an attractive strategy because it stimulates immune cells to target malignant cells by regulating the intrinsic activity of the immune system. However, due to lacking many immunologic markers, it remains difficult to treat glioma, a representative "cold" tumor. Herein, to wake the "hot" tumor immunity of glioma, Porphyromonas gingivalis (Pg) is customized with a coating to create an immunogenic tumor microenvironment and further prove the effect in combination with the immune checkpoint agent anti-PD-1, exhibiting elevated therapeutic efficacy. This is accomplished not by enhancing the delivery of PD-1 blockade to enhance the effect of immunotherapy, but by …

Enhanced Ctla-4 Blockade Anti-Tumor Immunity With Apg-157 Combination In A Murine Head And Neck Cancer, Daniel Sanghoon Shin, Saroj Basak, Mysore S Veena, Begoña Comin-Anduix, Arjun Bhattacharya, Tien S Dong, Albert Ko, Philip Han, Jonathan Jacobs, Neda A Moatamed, Luis Avila, Matteo Pellegrini, Marilene Wang, Eri S Srivatsan

Faculty, Staff and Student Publications

BACKGROUND: A phase I clinical study for patients with locally advanced H&N cancer with a new class of botanical drug APG-157 provided hints of potential synergy with immunotherapy. We sought to evaluate the efficacy of the combination of APG-157 and immune checkpoint inhibitors.

METHODS: CCL23, UM-SCC1 (human), and SCCVII (HPV-), MEER (HPV+) (murine) H&N cancer cell lines were utilized for in vitro and in vivo studies. We measured tumor growth by treating the mice with APG-157, anti-PD-1, and anti-CTLA-4 antibody combinations (8 groups). The tumor microenvironments were assessed by multi-color flow cytometry, immunohistochemistry, and RNA-seq analysis. Fecal microbiome was analyzed …

Mechanical Venous Thrombectomy For Deep Venous Thrombosis In Cancer Patients: A Single-Center Retrospective Study, Riya M Patel, Koustav Pal, Syed Hadi Ahmed, Joshua D Kuban, Milan Patel, Ketan Shah, Peiman Habibollahi, Zeyad Metwalli, Varshana Gurusamy, Sanjay Gupta, Cristhiam M Rojas-Hernandez, Vahid Afshar-Kharghan, Michael H Kroll, Rahul A Sheth

Faculty, Staff and Student Publications

PURPOSE: Venous thromboembolism (VTE) is a major contributor to the mortality of cancer patients. Mechanical thrombectomy (MT) is an endovascular technique that physically removes a thrombus without thrombolytics. The purpose of this study was to evaluate safety, efficacy, and clinical outcomes following MT for lower extremity DVT in cancer patients.

METHODS: This single-center, retrospective study evaluated outcomes following MT of lower extremity DVT in cancer patients from November 2019 to May 2023. The primary outcome measure was clinical success, defined as a decrease in Villalta score by at least 2 points following the intervention. Secondary outcomes included repeat intervention-free survival …

Modeling Acute Myocardial Infarction And Cardiac Fibrosis Using Human Induced Pluripotent Stem Cell-Derived Multi-Cellular Heart Organoids, Myeongjin Song, Da Bin Choi, Jeong Suk Im, Ye Na Song, Ji Hyun Kim, Hanbyeol Lee, Jieun An, Ami Kim, Hwan Choi, Joon-Chul Kim, Choongseong Han, Young Keul Jeon, Sung Joon Kim, Dong-Hun Woo

Faculty, Staff and Student Publications

Heart disease involves irreversible myocardial injury that leads to high morbidity and mortality rates. Numerous cell-based cardiac in vitro models have been proposed as complementary approaches to non-clinical animal research. However, most of these approaches struggle to accurately replicate adult human heart conditions, such as myocardial infarction and ventricular remodeling pathology. The intricate interplay between various cell types within the adult heart, including cardiomyocytes, fibroblasts, and endothelial cells, contributes to the complexity of most heart diseases. Consequently, the mechanisms behind heart disease induction cannot be attributed to a single-cell type. Thus, the use of multi-cellular models becomes essential for creating …

Mapping Recommendations Towards An Asian Code Against Cancer (Acac) As Part Of The World Code Against Cancer Framework: An Asian National Cancer Centers Alliance (Ancca) Initiative, Sok King Ong, Sarah K Abe, Gillian Li Gek Phua, Harindra Jayasekara, Kayo Togawa, Laureline Gatellier, Jeongseon Kim, Yawei Zhang, Siti Zuhrini Kahan, Siti Norbayah Yusof, Jong Soo Han, C S Pramesh, Manju Sengar, Abhishek Shankar, Clarito Cairo, Suleeporn Sangrajran, Erdenekhuu Nansalmaa, Tseveen Badamsuren, Tashi Dendup, Kinley Tshering, Jie He, R Soeko Werdi Nindito D, Azmi Ritana, Jeong Soo Im, Eun Young Park, Giang Nguyen Huong, Tran Thi Thanh Huong, Mohammed Biglari, Aasim Yusuf, Kishore Kumar Pradhananga, Champadeng Vongdala, Muhammad Taufiq Bin Jaafar, Nor Saleha Ibrahim Tamin, Yi Yi Myint, Kyaw Kan Kaung, Md Shafiur Rahman, Eshani Fernando, Beauta Rath, Babu Sukumaran, William Yk Hwang, Carolina Espina, Joachim Schüz, Manami Inoue, Tomohiro Matsuda

Faculty, Staff and Student Publications

This paper outlines the process undertaken by Asian National Cancer Centers Alliance (ANCCA) members in working towards an Asian Code Against Cancer (ACAC). The process involves: (i) identification of the criteria for selecting the existing set of national recommendations for ACAC (ii) compilation of existing national codes or recommendations on cancer prevention (iii) reviewing the scientific evidence on cancer risk factors in Asia and (iv) establishment of one or more ACAC under the World Code Against Cancer Framework. A matrix of national codes or key recommendations against cancer in ANCCA member countries is presented. These include taking actions to prevent …

Effect Of Delayed Cell Infusion In Patients With Large B-Cell Lymphoma Treated With Chimeric Antigen Receptor T-Cell Therapy, Andrew P Jallouk, Naishu Kui, Ryan Sun, Jason R Westin, Raphael E Steiner, Ranjit Nair, Loretta J Nastoupil, Luis E Fayad, Ajlan Al Zaki, Misha Hawkins, Sherry Adkins, Mansoor Noorani, Kaberi Das, Jared Henderson, Elizabeth J Shpall, Partow Kebriaei, Jeremy Ramdial, Christopher R Flowers, Sattva S Neelapu, Sairah Ahmed, Paolo Strati

Faculty, Staff and Student Publications

Complications occurring after lymphodepleting chemotherapy (LDC) may delay chimeric antigen receptor (CAR) T-cell infusion. The effect of these delays on clinical outcomes is unclear. We performed a retrospective analysis of 240 patients with relapsed/refractory large B-cell lymphoma treated with standard-of-care axicabtagene ciloleucel (axi-cel) and identified 40 patients (16.7%) who had delay in axi-cel infusion. Of these, 85% had delay due to infection. At time of LDC initiation, patients with delayed infusion had lower absolute neutrophil count (P=0.006), lower platelets (P=0.004), lower hemoglobin (P5 days (4.6 vs. 8.2 months; P=0.036), but not 1 day (5.7 vs. 8.2 months; P=0.238). Following propensity …

Plant Model Of Α-Synucleinopathy: Expression Of Α-Synuclein A53t Variant In Hairy Root Cultures Leads To Proteostatic Stress And Dysregulation Of Iron Metabolism, Jasmina Kurepa, Kristen A. Bruce, Greg A. Gerhardt, Jan A. Smalle

Neurology Faculty Publications

Synucleinopathies, typified by Parkinson’s disease (PD), entail the accumulation of α- synuclein (αSyn) aggregates in nerve cells. Various αSyn mutants, including the αSyn A53T variant linked to early-onset PD, increase the propensity for αSyn aggregate formation. In addition to disrupting protein homeostasis and inducing proteostatic stress, the aggregation of αSyn in PD is associated with an imbalance in iron metabolism, which increases the generation of reactive oxygen species and causes oxidative stress. This study explored the impact of αSyn A53T expression in transgenic hairy roots of four medicinal plants (Lobelia cardinalis, Artemisia annua, Salvia miltiorrhiza, and Polygonum multiflorum). In all …

Neurocorrelates Of Character Choice In A Videogame Task, Elijah Nieman

All NMU Master's Theses

An individual’s experience of autonomy or lack thereof has a significant effect on interest and motivation towards everyday activities. The present study looks to expand on basic research by examining the neurocorrelates of interest following supporting and thwarting of autonomy through character choice in a videogame task. Previous research has demonstrated an important role of digital avatars on perceived autonomy and interest, and early neurological results have shown that choice of visual frame affects reward processing of task outcomes. Together, autonomy supports seem to enhance engagement and interest in a situation. In the present work, participants were presented with five …

Evaluating Embryo Survival Of Surrogate Fish Species Using Multiple Incubators: Continuing Efforts Towards Reintroducing Arctic Grayling (Thymallus Arcticus) Into Lower Michigan Streams, Joshua Mutchler

All NMU Master's Theses

Arctic Grayling Thymallus arcticus have been successfully reintroduced to the Upper Missouri River Basin of Montana using Remote Site Incubators (RSI). Widespread use of RSIs as part of reintroduction efforts in Michigan is challenged by low gradient streams, which limit deployment success. In this study, I evaluated the utility of an alternative instream rearing device, the Floating Basket Incubator (FBI), by directly comparing the survival of Rainbow Trout Oncorhynchus mykiss, Brook Trout Salvelinus fontinalis, and Walleye Sander vitreus (as Arctic Grayling surrogates) between RSI and FBI in stream and hatchery environments. In addition, I assessed how abiotic and …

Occupational Therapy Services In Pediatric Mental Health, Hallie Jerome

Occupational Therapy Capstone Presentations

Children and youth experiencing mental health concerns often have limited or decreased engagement in meaningful occupations. Additionally, children and youth who experience mental health concerns at an early age are more likely to experience negative health outcomes such as poor occupational engagement, academic achievement, adverse childhood experiences (ACES), maltreatment/abuse/neglect, family and peer conflict/rejection, low self-esteem, early substance use, and many others impacting their overall health and well-being. My capstone project aimed to administer occupation-based interventions in a pediatric mental health program while I advance my skills working with an interdisciplinary team. The literature and needs assessment indicated a need for …

Mems Optical Cavities For Low Power Communication., Jacob Schopp

Electronic Theses and Dissertations

This thesis discusses the use of MEMS (microelectromechanical systems) optical cavities for use in low power communication. MEMS optical cavities are thin air gaps between an actuated MEMS mirror and a fixed glass encapsulation. By moving the MEMS mirror the reflectivity of the MEMS optical cavity can be changed. This change in reflectivity can be used to transmit information passively by modulating reflected light intensity instead of generating new light using a light source. These MEMS optical cavities are demonstrated using optical fibers for illumination delivery and reflected light collection. In addition to being reflected for communication, the illuminating light …

Acoustofluidic Delivery Of Biomolecules To Cells: A Novel Approach For Preparing Car-T Cells., Madison C. Colebank

Electronic Theses and Dissertations

The continuous pursuit of effective methods for gene transfection in primary human T cells is crucial for advancing cell-based therapies. Viral vectors, currently used in cell-based therapies, involve a lengthy and costly process. This thesis explores the efficacy of coupling acoustofluidic and sonoporation techniques as an alternative non-viral gene transfection approach with primary T cells. Sonoporation, in conjunction with microbubbles, has been demonstrated to induce cavitation, resulting in the formation of pores in the cell membrane through which molecules can enter. Using flow cytometry, we compared the loading efficiency of primary T cells with that of Jurkat T cells to …

The Evaluation Of Compound X In A Mouse Model In Chikv Disease, Kaitlyn Blotter

Undergraduate Honors Capstone Projects

Chikungunya virus (CHIKV) is an infectious alphavirus spread by Aedes aegypti and Aedes alpopictus mosquitoes. Recently, there have been various outbreaks in the Americas, the Caribbean, and across Asia and Africa (Bettis et al., 2022). Although mortality rates of Chikungunya disease are relatively low, the disease has been shown to have high morbidity and chronic effects. Currently, there are no approved antivirals for treatment of CHIKV infection. The goal of this study was to identify whether Compound X is an effective treatment in a mouse model of CHIKV infection. Compound X was active in cell culture models of CHIKV infection, …

An Investigation Of Protein Arginine Methyltransferase 1 Substrate Recognition Through Ribonucleoprotein Modifications, Isabella Beverly Lonardo

Undergraduate Honors Capstone Projects

Just as humans need to communicate with each other, our cells have a series of processes that allows them to communicate from one to another. Protein Arginine Methyltransferase I is an enzyme that helps mediate cellular communication by selectively binding to target proteins and performing chemistry that has broad-reaching downstream effects. However, the mechanisms that underly PRMT1 selectivity have yet to be elucidated. This study uses alterations to a series of known targets, called ribonucleoproteins, to identify some of the characteristics that govern recognition by the PRMT1 enzyme. I have made significant progress in the creation, expression, and purification of …