Digital Commons Network^™

Pipecolic Acid And Novel Insights Into Cerebral Malaria, Akua E. Mensah

Theses and Dissertations

Cerebral malaria (CM), a severe manifestation of Plasmodium infection, prompts our investigation into the nuanced role of pipecolic acid in its pathophysiology. To unravel the molecular intricacies, we conducted in vitro lysine labeling techniques of mice infected with P. berghei ANKA parasites, and human P. falciparum grown in vitro, aiming to discern the impact of Plasmodium on pipecolic acid production. Previous observations indicated an elevation in pipecolic acid levels correlating with neurological decline in children with CM. In our study, confirming elevated pipecolic acid presence in the plasma and brain tissues of CM patients and the animal model of CM, …

Systematics And Biogeography Of Shallow Subterranean Carabidae In The Eastern United States, Curt Harden

All Dissertations

The shallow subterranean habitats (SSHs) of the eastern United States are reviewed, along with the associated ground beetles (Carabidae). Leaf litter, deep soil, fractured rock layers, and terrestrial epikarst are the predominant SSHs inhabited by Carabidae in the region. The long enigmatic genus Horologion is found, through study of morphology and DNA sequence data, to represent a paleoendemic lineage distributed only in West Virginia and Virginia; the likely sister to Horologion is the Gondwanan tribe Bembidarenini. A new species, Horologion hubbardi sp. n., is described from several individuals collected in or near drip pools in caves; the beetles probably …

Evaluation Of An Emergency Department Provider Information Update On The Initiation Of Suboxone With A Community Treatment Program Referral For Opioid Overdose And Opioid Use Disorder, Carmen Brewington

All Dissertations

Over 21 million people in the United States (U.S.) require substance use disorder (SUD) treatment; yet less than 4 million people receive treatment (Bressan & Kening, 2023). According to Thomas et al. (2022), opioid overdose deaths are increasing, and improving access to evidence-based treatment is critical. Hospital emergency departments (EDs) play a lifesaving in the emergency treatment of opioid overdoses; but can also serve as gateways to opioid treatment by healthcare providers for opioid addictions (Reuter et al., 2022). Mortality, after an ED visit for opioid overdose, is high (Thomas et al., 2022). Community resources are limited, thus making it …

Chm116 Principles Of Chemistry Ii Lecture Slides, Leah S. Cohen, Probal Banerjee

Open Educational Resources

This file contains the lecture slides that are used in CHM116, Principles of Chemistry II.

Nationwide Practices And Perspectives On Early Literacy Activities In Daycare, Analaura Amador

Open Access Theses & Dissertations

No abstract provided.

Bcl2 Mediated Targeted Drug Delivery For The Treatment Of Kidney Fibrosis And Stomach Cancer, Humayra Afrin

Open Access Theses & Dissertations

Apoptosis, the programmed death of cells, is primarily regulated by a delicate balance between pro-apoptotic and anti-apoptotic signals. The Bcl-2 (B-cell lymphoma 2) family of proteins acts as anti-apoptotic agents, promoting cell survival. Dysregulation of these proteins is a common occurrence in conditions such as cancer and fibrosis, where overexpression of anti-apoptotic members can foster tumor cell survival and fibroblast activation. In this study, our aim was to explore the therapeutic potential of Bcl-2 inhibitors, both as a small molecule (specifically Navitoclax (Navi)), inhibitor and as Bcl-2 siRNA, for targeted treatment. Intravenous administration of Navi often leads to thrombocytopenia, necessitating …

Talking About Sex: The Relationship Between Cultural Constructs And Sexual Behaviors Among Latina Emerging Adults, Ariana Cervantes-Borges

Open Access Theses & Dissertations

Emerging adults account for a significant portion of yearly STI cases with Latinxs living in border regions often being disproportionally affected. Moreover, Latinas may hold much of the responsibility for sexual health when compared to their male counterparts; however, culture may serve as both a protective and risk factor for deleterious sexual behaviors. These disparities highlight the importance of assessing Latina sexual risk and to a further extent, perceptions of sexual and reproductive health services. The present study assessed how cultural constructs were associated with sexual risk and STI testing views among Latinas and how these associations may be moderated …

Sulfonated Polyethersulfone Membranes For Electrodialysis Desalination And The Influence Of Solvent Evaporation On Current Efficiency, Salinity Reduction, And Permselectivity, Li Chen

Open Access Theses & Dissertations

The high cost of ion exchange membranes significantly limits the public application of electrodialysis. The research of novel, inexpensive ion exchange membranes is essential to developing and applying electrodialysis desalination technology. This research focuses on fabricating cation exchange membranes with polyethersulfone (PES) and sulfonated PES (sPES) for water treatment. N-Methyl-2-Pyrrolidone (NMP) was used as an organic solvent to dissolve PES. After different solvent evaporation times were optimized from 0 hr to 24 hr, those membranes were formed through the phase inversion technique. The performance results show that the PES membranes performed the best when the solvent evaporated at 3 hr, …

Water Sufficiency For Organismal Function In Dryland Critical Zones, Lindsey Dacey

Open Access Theses & Dissertations

Water availability is crucial for organismal survival and growth in dryland environments, affecting both ecological interactions and carbon dynamics. The goal of this thesis is to develop soil water release curves (SWRCs) that link soil water potentials (Ψ) to soil water content (θ). Using the SWRCs, temporal soil water sufficiency curves are developed, which quantify the amount of time that dryland critical zones have enough water to sustain the physiology of organisms. These curves allow for effectively indicating water availability across different species, coverage types, and soil conditions, enhancing our understanding of water dynamics in drylands and contributing important parameters …

Biofabrication Of Human Tissue-On-A-Chip Models Using Engineered Biocompatible Electrospun Scaffolds, Zayra Naomi Dorado

Open Access Theses & Dissertations

This study explored the adoption of furfuryl gelatin (F-gelatin) based electrospun scaffolds compared with poly-caprolactone (PCL) as promising biomaterials for tissue engineering applications. Tissue-on-a-chip models, incorporating F-gelatin and PCL electrospun scaffolds, offer promising avenues for healthy and disease-in-vitro tissue models that can be explored to investigate underlying physiological mechanisms involved in disease development. Previous research has demonstrated the cytocompatibility of F-gelatin when used for modifying implant surfaces and tissue repair applications [1]. Our earlier published works have also successfully utilized F-gelatin for in-vitro cardiac tissue engineering [2][3]. We designed F-gelatin and PCL electrospun scaffolds to replicate the native tissue extracellular …

Prelimbic Cortex Neuronal Assemblies In Behaviors Predictive Of Drug-Seeking Before And After Cocaine Self-Administration, Karla J. Galvan

Open Access Theses & Dissertations

Literature has established that there are certain behaviors that are predictive of drug seeking and relapse such as impulsivity, distress tolerance (DT), Pavlovian conditioned approach (PCA), anxiety, and sensation-seeking. However, few studies have examined the behavioral interactions among these tasks and drug-seeking, and none have examined the neural interactions in the prelimbic cortex, which has an essential role in drug-seeking and is implicated in each of the aforementioned behaviors of interest.We therefore hypothesized that certain behaviors including high impulsivity, low distress tolerance, high anxiety, and high locomotor activity would predict high cocaine-seeking. We further hypothesized that the neuronal activity in …

Assessing Nordihydroguaiaretic Acid Properties And Its Potential Therapeutic Effect For Glioblastoma, Jose Arturo Guerrero

Open Access Theses & Dissertations

This study employs a combination of theoretical and experimental analysis to spectroscopically investigate the biomechanistic structure relationship and therapeutic effects of the Nordihydroguaiaretic Acid (NDGA) chemical derived from the Larrea Tridentata plant. These relationships are crucial for understanding NDGA's efficacy in disease prevention, treatment, and potential toxicological effects. While the medicinal and antiviral properties of the NDGA have been studied extensively, there remains a gap in optically identifying and reporting its structural changes. The current research successfully reveals evident trends in NDGA's vibrational signatures, particularly highlighting the absence of the Raman feature at 780 〖cm〗^(-1) as indicative of a fully …

Applying Multi-Scale Computational Approaches To Study Disease Related Biomolecules, Wenhan Guo

Open Access Theses & Dissertations

Computational biophysics plays a significant role in understanding biological processes in various biology systems and provides new sights to investigate disease-related biomolecules. During the doctoral research, I utilized multi-scale computational approaches, including structural modeling, Molecular Dynamics (MD) simulation, electrostatic analysis (DelPhi, DelPhiForce), and machine learning based Hybridizing Ions Treatment-2 (HIT-2) program, to investigate biomolecules. My research includes bound ions effects on kinesin Ncd binding to microtubule, ion concentration effects on kinesin BimC binding affinity, microtubule dynamics, etc. Ions are crucial for biomolecular interactions, especially for highly charged biomolecules. Bound ion effects are difficult to study in implicit solvent models. Based …

Our Author Is Crazy, Zani A. Meaders

Open Access Theses & Dissertations

This thesis delves into the realm of young adult (YA) fantasy literature, exploring how its meta-narrative elements, fast-paced story beats, and relatable characters can facilitate the acquisition of healthy coping mechanisms among its readers. In the preface, I discuss the importance of providing YA audiences with narratives that not only entertain but also serve as tools for navigating the complexities of adolescence. Drawing upon psychological theories and literary analysis, I argue that meta-awareness in YA fantasy can offer readers a unique perspective on their own struggles and encourage them to develop resilience and coping skills.

The accompanying YA Fantasy story …

Combining Green Analytical Methods And Machine Learning To Develop Urinary Fatty Acid Models For Cancer Detection, Elizabeth Noriega Landa

Open Access Theses & Dissertations

This study aimed to explore fatty acids (FAs) as non-invasive biomarkers for prostate cancer (PCa) detection and prognosis, and their potential applications in other cancers. The objectives of this study were: 1) Develop a non-invasive urinary FAs-based model for diagnosing PCa; 2) Develop a FAs-based liquid biopsy model for non-invasive monitoring of PCa progression; 3) Investigate FAs composition in periprostatic adipose tissue (PPAT) collected from PCa positive patients; 4) Investigate the potential of urinary FAs biomarkers for diagnosing clear cell renal cell carcinoma (ccRCC) and ovarian cancer (OC).For the PCa diagnostic model, urine samples from 334 biopsy-confirmed PCa-positive and 232 …

Establishing The Validity Of A Measure Of Implicit Bias Toward People With Alcohol Use Disorder, Erin Marie Portillo

Open Access Theses & Dissertations

Alcohol use disorder has been identified as one of the major burdens of disease but also remains one of the disorders with the lowest treatment prevalence. For individuals who seek treatment for their alcohol use disorder, they may experience barriers that impact their treatment for alcohol use disorder. These barriers may extend to a person’s beliefs, attitudes, and stigma. While attitudes and beliefs refer to their perceived need for treatment and beliefs of self-reliance, stigma is multistep process that makes way for stereotypes, prejudice, and acts of discrimination to take place. With labels such as “alcoholic” implying negative connotation and …

Characterization Of The Androgen Receptor H1-H3 Loop As A Putative Fkbp Regulatory Surface, Isela Rodriguez

Open Access Theses & Dissertations

Prostate Cancer (PCa) is one of the most common life-threatening malignancies diagnosed among American men. Initiation and progression of PCa are dependent upon androgen receptor (AR) regulated genes. Functional receptor conformation is influenced by the cooperation of chaperone and cochaperone proteins including the 52 and 51 kDa FK506 binding proteins (FKBP52 and FKBP51). FKBP52 is known for being a positive regulator of AR, PR (progesterone receptor), and GR (glucocorticoid receptor) activity, whereas FKBP51 negatively regulates steroid hormone receptor activity. As a result, these two proteins have become highly promising therapeutic targets for the disruption of mechanisms important in several endocrine-related …

Evaluating The Effectiveness Of A Resilience Intervention On College Students' Mental Illness And Subsequent Alcohol Use, Aitiana Ivonne Sanchez-Garciaguirre

Open Access Theses & Dissertations

Mental illness and stigma surrounding mental illness are common among college-aged individuals. Alcohol use is also common among college-aged individuals, and often coexists with increased symptoms of mental illness, leading to unhealthy perpetual coping mechanisms. However, increased resilience and adaptive coping strategies may suppress the need to use alcohol as a coping mechanism when experiencing symptoms of mental illness and the stigma associated with it. The present study sought to utilize a resilience intervention to increase resilience and adaptive coping strategies among Hispanic college students to reduce their alcohol use and experiences of depression and/or anxiety. Additionally, the present study …

Produced Water: Characterization And Treatment, Ramon Antonio Sanchez Rosario

Open Access Theses & Dissertations

In recent years, environmental concerns have urged companies in the energy sector to modify their industrial activities to facilitate greater environmental stewardship. For example, the practice of unconventional oil and gas extraction has drawn the ire of regulators and various environmental groups due to its reliance on millions of barrels of fresh water for hydraulic fracturing well stimulation, which is generally withdrawn from natural sources and public water supplies. Additionally, this process generates two substantial waste streams, which are collectively characterized as flowback and produced water. Whereas flowback water is comprised of various chemical additives that are used during hydraulic …

Characterizing The Mentoring Landscape In An Online Biology Cure: An Examination Of Student And Graduate Teaching Assistant Perspectives And Outcomes, Karen Alejandra Santillan

Open Access Theses & Dissertations

Both undergraduate research experiences (UREs) and course-based undergraduate research experiences (CUREs) provide students with benefits and opportunities such as improving technical skills and making connections that provide resources. Where these two experiences differ is accessibility. Similar to their URE counterpart, CUREs entail mentorship on the part of the faculty/graduate teaching assistant (GTA) instructor (Dolan, 2016). There is general agreement that mentoring has many benefits for undergraduate STEM students, such as an improvement in retention rates, increased confidence and self-efficacy, and a more established sense of belonging and science identity (Dolan, 2016). CURE instructors often adopt the role of “mentor” in …

Atlas-Based Analysis Of The Neural Projections From The Lateral Hypothalamic Area To The Lower Brainstem In The Adult Male Rat, Jessica Valeria Salcido Padilla

Open Access Theses & Dissertations

Within the last decade, the number of diseases associated with dysregulation of the neural circuits responsible for energy balance, glucosensing, and feeding control has increased. One brain region containing these circuits is the lateral hypothalamic area (LHA), which plays an important role in the sleep/wake cycle, energy balance, and motivated behavior. Although the LHA has been termed a â??feeding centerâ??, other regions within the hindbrain are known for the regulation of similar functions including energy balance, glucosensing, and feeding control. While many studies have explored and reported projections from the LHA to the hindbrain, few have further investigated the interconnections …

Selective Transfection Of A Transferrin Receptor-Expressing Cell Line With Dna-Lipid Nanoparticles And Synthesis Of Parasite-Derived Glycans As Biomarkers For Leishmaniasis, Irodiel Vinales Lozano

Open Access Theses & Dissertations

Despite notable progress in lipid nanoparticle (LNP)-mediated gene delivery, achieving selective transfection of specific cell types, such as cancer cells, remains a significant hurdle, hindering the advancement of innovative gene therapies. In this study, we engineered an LNP formulation encapsulating plasmid DNA (pDNA) encoding the monomeric Green Lantern (mGL) fluorescent reporter protein. The DT7 peptide ligand targeting human transferrin receptor 1 (hTfR1) was also conjugated to the LNP surface for targeted delivery to hTfR1-expressing cells. Optimization of LNP composition yielded favorable particle diameter, ζ-potential, yield, and pDNA encapsulation efficiency. Evaluation of transfection selectivity using a panel of two engineered cell …

Synthesis And Characterization Of Indole-Based Zinc Dipyrrin Photosensitizers, Jean-Pierre Sanza

Electronic Theses and Dissertations

Metal complexes of dipyrromethene (dipyrrins) used as sensitizers in photocatalysis offer a way to harness solar energy in chemical bonds to create new fuels. This offers the dual role of reducing fossil fuel dependence and atmospheric CO₂ levels. Traditionally, metal dipyrrin complexes are synthesized using substituted pyrroles, aldehydes, and transition metals. Indoles have a more expanded pi-electron system and their dipyrrin-type complex may exhibit visible light absorption, suggesting that they can act as photosensitizers for CO₂ reduction processes. A novel indoledipyrromethene was synthesized using unsubstituted indole and mesitaldehyde. The complex exhibits visible light absorption at 422 nm. Its …

Conversion Of Microcrystalline Cellulose To Hexane Using Hydrogenated Metal Oxides At Low Temperature And Pressure, Mubarak Osman

Electronic Theses and Dissertations

Increasing global energy demand and environmental concerns have fueled the exploration of sustainable and efficient methods for renewable fuel production. The conversion of cellulosic waste to hydrocarbon fuels using hydrogenated metal oxides presents a novel and eco-friendly approach to sustainably address energy demand. In this study, hydrogenated metal oxides were used as catalysts in a relatively low-temperature and atmospheric-pressure environment to facilitate the conversion of cellulosic waste into hydrocarbon fuels. Hydrogenated metal oxides have been introduced as potential hydrogen transfer catalysts. The expected result is the selective conversion of cellulose to hexane at relatively low temperatures, which significantly reduces energy …

A Comparison Of Student Academic Variables In Mixed Teaching Methods And Traditionally Taught Biology Courses At Participating Community Colleges In Tennessee, Elesha Goodfriend

Electronic Theses and Dissertations

The primary purpose of this study was to compare academic variables from students participating in mixed teaching methods/course revitalization biology courses to those in traditional courses at two community colleges in Tennessee. A two-group comparison design using archived data was used, which evaluated differences in final grades, exam scores, and homework completion as indicators of the academic success between an urban institution and a rural college. A power analysis was performed using G*Power software to provide the needed sample size, indicating a size of 176. There were 227 students in the active teaching lecture classrooms, and 198 students in the …

In A State Of Becoming, Benjamin Conley

Electronic Theses and Dissertations

The artist discusses his Master of Fine Arts exhibition titled, In a State of Becoming. The exhibition was on view at the Tipton Gallery in downtown Johnson City, TN from February 26 March 8, 2024. In a State of Becoming showcased three large scale paintings, five multimedia prints, two sculptural installations, and a video projection installation. Conley's thesis research and current artistic practice revolve around the interfaces, connections, and relationships of humans and animals. Conley explicitly uses language like "animal" to describe "non-human animals" in his work's context. The exhibited works focused primarily on how the artist and/or the viewer …

Keeping It In The Ground: An Analysis Of Colorado Oil And Gas Law And The Legal Tools To Limit Production, Colin Hull

Colorado Environmental Law Journal

No abstract provided.

Neural Network Characterization Methods For Noisy Quantum Hardware Results, Daniel Richard Breslawski

Theses

As the nascent quantum computing paradigm matures and quantum devices become widely available, discrete "eras" are emerging which characterize evolving quantum computing technologies, much like semiconductor technology node classifications. The current period has been coined the Noisy Intermediate-Scale Quantum (NISQ) era, as devices above 50 qubits in size exist but computations using these devices accumulate error quickly from classical interference sources and state decoherence. NISQ machines may surpass the capabilities of modern classical computers in ideal circumstances, but the accumulation of error from physical noise limits the size and implementability of reliable quantum algorithms. Because of these limitations, strategies are …

Investigation On The Synthesis And Characterization Of Novel Phase Change Materials For Packaging Application, Sriram Srinivasan

Theses

Phase change material (PCM) is one of the majorly growing techniques used for thermal heat storage in various fields including construction for insulation and packaging industry. PCMs are widely used to increase the shelf life of the produce and prevent from harsh climatic conditions during transportation. In this present investigation, a PCM system was developed in the form of microcapsules using lauric acid (LA) and capric acid (CA) emulsions in melamine formaldehyde (MF) solution and polyurethane foam (PUF) using polytetrahydrofuran, isoprene diisocyanate, and polyethylene glycol (PEG). Acetic acid and triethylamine were used to control the pH during microcapsule preparation. The …

Adipose Stem Cell Response To Borophosphate Bioactive Glass, Nada A. Abokefa, Bradley A. Bromet, Rebekah L. Blatt, Makenna S. Pickett, Richard K. Brow, Julie A. Semon

Materials Science and Engineering Faculty Research & Creative Works

Silicate and Borate Bioactive Glasses Have Been Reported to Create Alkaline Conditions by Rapidly Releasing Ions When Reacting in Aqueous Solution. at Certain Levels, This Alkaline Solution Can Negatively Affect Cell Viability. Adding Phosphate Ions to the Glass Composition Can Control the Degradation Rate of Bioactive Glasses and Create a Neutral PH Environment. This Study Evaluated a Series of Boro phosphate Bioactive Glasses (BPBGs) with Nominal Molar Compositions 16Na₂O_-24CaO-XB₂O₃-(60-X) P₂O₅, Where X = 0, 40, or 60. the Phosphate (X0) Glass (PBG) Produced an Acidic Solution When Dissolved …