Digital Commons Network^™

Fabrication Of Two-Dimensional Material-Based Nano-Capacitors Using Bismuth Selenite (Bi2seo5) To Study Its Dielectric Properties, Major Kc

McKelvey School of Engineering Graduate Student Theses & Dissertations

In recent years, the demand for high-performance micro and nanodevices has surged, necessitating the exploration of novel dielectric materials to replace conventional silicon dioxide. Following the continuation of the Moorse law, as device dimensions reduce to nanoscale levels, the properties of silicon dioxide can degrade, leading to issues such as increased leakage current and reduced gate control. Materials with superior electrical properties, such as higher dielectric constant, lower leakage current, and better thermal stability allowing for the development of faster, more efficient, and more reliable devices are in higher demand than ever. Two-dimensional layered semiconductor nanomaterials represented by compounds such …

Tracking And Mitigating Imprint Erasure During Human Pluripotent State Transitions, Laura A. Fischer

Arts & Sciences Graduate Student Theses and Dissertations

Early human embryogenesis is difficult to study due to practical and ethical limitations, but the successful culture of “naïve” human pluripotent stem cells (hPSCs) has expanded our ability to model cells akin to the pre-implantation epiblast. Naïve hPSCs are readily obtained through in vitro resetting of “primed” hPSCs, cells which model the post-implantation epiblast. Naïve hPSCs offer the advantages of modeling the pre-implantation transcriptome and epigenome, the ability to form embryonic and extraembryonic tissues, and the capacity to self-organize into integrated human embryo models. However, a persistent drawback of naïve hPSCs is the loss of parent-specific epigenetic marks, called imprints. …

Outpatient Administration Of Car T-Cell Therapy: A Focused Review With Recommendations For Implementation In Community Based Centers, Ariel Perez, Tiba Al Sagheer, George Nahas, Yuliya Linhares

All Publications

Chimeric Antigen Receptor T-cell (CAR-T) therapy has transformed the treatment landscape for hematological malignancies, showing high efficacy in patients with relapsed or refractory (R/R) disease and otherwise poor prognosis in the pre-CAR-T era. These therapies have been usually administered in the inpatient setting due to the risk of cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). However, there is a growing interest in the transition to outpatient administration due to multiple reasons. We review available evidence regarding safety and feasibility of outpatient administration of CD19 targeted and BCMA targeted CAR T-cell therapy with an emphasis on the …

Dredging The Wetlands, Tilling The Field: Ecotonal Poetics Of The Anthropocene, Stacey Balkun

Electronic Theses and Dissertations

This dissertation interrogates the use of poetic fragmentation as a tool of ecological representation and coins the term “ecotonal poetics.” Ecotonal poesis, this dissertation explains, is the stretching of lyric across the field of the page, using space, sound, language, and parataxis in its attempt to not only represent but create ecotonal ecologies through text, sound, and visual cues. This dissertation puts forth four case studies of contemporary American women poets turning the space of the page into an ecotone where text, white space, author, reader, body, and environment meet. The poets studied here rely heavily on textual experimentation, suggesting …

Beth Bailey Art399 Portfilio, Beth Bailey

Professional Practices (ART 399)

My photographic work documents the landscape and plants in rural Western Kentucky and Tennessee. Through botanical specimens, such as flowers, I show the beauty of this area. I contemplate the influence of humans and the transformation of previously untouched land. Through landscape photography, in color with black and white film and alternative processes, I seek natural places that provide a calm and stable feeling.

Exploring the relationships between objects, considering the lines they create or the interplay of soft shadows, reveals a play of rough and soft textures, accentuated by mid-tones—a shared element with the three chosen artists. Many of …

Beyond The Tap: The Flint, Michigan Water Crisis As An Issue Of Environmental Racism, Vincent J. Vitanza

Student Theses 2015-Present

The following paper provides insight into the Flint, Michigan water crisis of 2014 through the lens of environmental racism. The Flint, Michigan water crisis saw the contamination of the municipal water supply of the town of Flint as a result of the decision to switch the main water source from Lake Huron to the Flint River as a temporary measure as the town of Flint switched to the Karegnondi Water Authority contract to supply their municipal water. Flint is a majority minority community, and the facets of race and socioeconomic status played a significant role in how the crisis was …

Autism Risk Gene Cul3 Alters Neuronal Morphology Via Caspase-3 Activity In Mouse Hippocampal Neurons, Qiang-Qiang Xia, Anju Singh, Jing Wang, Zhong Xin Xuan, Jeffrey D. Singer, Craig M. Powell

Biology Faculty Publications and Presentations

Autism Spectrum Disorders (ASDs) are neurodevelopmental disorders (NDDs) in which children display differences in social interaction/communication and repetitive stereotyped behaviors along with variable associated features. Cul3, a gene linked to ASD, encodes CUL3 (CULLIN-3), a protein that serves as a key component of a ubiquitin ligase complex with unclear function in neurons. Cul3 homozygous deletion in mice is embryonic lethal; thus, we examine the role of Cul3 deletion in early synapse development and neuronal morphology in hippocampal primary neuronal cultures. Homozygous deletion of Cul3 significantly decreased dendritic complexity and dendritic length, as well as axon formation. Synaptic spine density significantly …

Perinuclear Damage From Nuclear Envelope Deterioration Elicits Stress Responses That Contribute To Lmna Cardiomyopathy, Kunal Sikder, Elizabeth Phillips, Zhijiu Zhong, Nadan Wang, Jasmine Saunders, David Mothy, Andrew Kossenkov, Timothy Schneider, Zuzana Nichtova, György Csordás, Kenneth B. Margulies, Jason C. Choi

Center for Translational Medicine Faculty Papers

Mutations in the LMNA gene encoding lamins A/C cause an array of tissue-selective diseases, with the heart being the most commonly affected organ. Despite progress in understanding the perturbations emanating from LMNA mutations, an integrative understanding of the pathogenesis underlying cardiac dysfunction remains elusive. Using a novel conditional deletion model capable of translatome profiling, we observed that cardiomyocyte-specific Lmna deletion in adult mice led to rapid cardiomyopathy with pathological remodeling. Before cardiac dysfunction, Lmna-deleted cardiomyocytes displayed nuclear abnormalities, Golgi dilation/fragmentation, and CREB3-mediated stress activation. Translatome profiling identified MED25 activation, a transcriptional cofactor that regulates Golgi stress. Autophagy is disrupted …

Immunopathogenesis Of Post-Infectious Hydrocephalus, Sascha Powers Bernier

Honors Scholar Theses

Hydrocephalus is characterized by the abnormal accumulation of cerebrospinal fluid (CSF) within the brain ventricles. In post-infectious hydrocephalus (PIH) cases, the condition presents challenges in understanding the immune response. PIH is a complex condition, often persisting after the initial infection is treated and thus requiring a deeper understanding of the immune mechanisms involved in its development. This thesis will explore the immunopathogenesis of PIH, elucidating the relationship between the immune response and neurological complications that would succeed infection. The immune response of PIH includes a series of events, beginning with the activation of immune cells and finishing with the release …

Testing The Hypothesis That Tennis Points Are Independent And Identically Distributed Using Statistical Methods, Ernesto Ugona Santana

Senior Honors Theses

Most research on the probability of winning a tennis match is based on the assumption that the points are independent and identically distributed, treating each point as a Bernoulli trial with fixed probability of success. This assumption, however, seems to contradict experience. Players' performance appears to fluctuate as the match progresses due to the psychological effect of past performance. To test this counterintuitive yet central assumption, previous research has attempted to test the independence hypothesis. However, there exists a research gap in evaluating the identicality-of-distribution hypothesis, a question of broader scope than that of independence. Hence, the purpose of this …

Influence Of Four Veterinary Antibiotics On Constructed Treatment Wetland Nitrogen Transformation, Matthew V. Russell, Tiffany L. Messer, Deborah A. Repert, Richard L. Smith, Shannon L. Bartelt-Hunt, Daniel D. Snow, Ariel P. Reed

Department of Civil and Environmental Engineering: Faculty Publications

The use of wetlands as a treatment approach for nitrogen in runoff is a common practice in agroecosystems. However, nitrate is not the sole constituent present in agricultural runoff and other biologically active contaminants have the potential to affect nitrate removal efficiency. In this study, the impacts of the combined effects of four common veterinary antibiotics (chlortetracycline, sulfamethazine, lincomycin, monensin) on nitrate-N treatment efficiency in saturated sediments and wetlands were evaluated in a coupled microcosm/mesocosm scale experiment. Veterinary antibiotics were hypothesized to significantly impact nitrogen speciation (e.g., nitrate and ammonium) and nitrogen uptake and transformation processes (e.g., plant uptake and …

Beyond The Iudex Threshold: Human Oversight As The Conscience Of Machine Learning, E. Jason Albert, Jessica E. Brown

Colorado Technology Law Journal

No abstract provided.

Nutrition, Obesity, And Seborrheic Dermatitis: Systematic Review, Emily Woolhiser, Noah Keime, Arya Patel, Isaac Weber, Madeline Adelman, Robert P. Dellavalle

Student Publications

Background:

Pathogenesis of seborrheic dermatitis involves lipid secretion by sebaceous glands, Malassezia colonization, and an inflammatory response with skin barrier disruption. Each of these pathways could be modulated by diet, obesity, and nutritional supplements. Current treatment options provide only temporary control of the condition; thus, it is essential to recognize modifiable lifestyle factors that may play a role in determining disease severity.

Objective:

This study aimed to summarize published evidence on diet, nutritional supplements, alcohol, obesity, and micronutrients in patients with seborrheic dermatitis and to provide useful insights into areas of further research.

Methods:

A literature search of Scopus, PubMed, …

Cardiomyocyte-Specific Modified Mrna For Myocardial Regeneration In A Porcine Myocardial Infarction Model, Jiacheng Sun

All ETDs from UAB

The inability of adult mammalian cardiomyocytes (CMs) to undergo cell renewal following ischemic injury stands as a major impediment to heart function recovery and contributes to heart failure (HF). Current therapeutic approaches do not offer an effective approach of reactivating CM cell division to prevent HF in humans. Recognizing this challenge, gene therapy has emerged as a promising approach to promote CM proliferation and enhance cardiac repair. A particularly notable approach is the utilization of modified mRNA (modRNA), which provides a safe, controlled, transient, non-viral, and clinically relevant means of gene delivery. The study was conducted using a novel cardiomyocyte …

Synthesis Of High-Quality Cost Effective Zno Nanorods For Photovoltaic Applications, Ghusoon Jalil Ibrahim Al Bazzar

Theses and Dissertations

Due to its outstanding structural, optical, and electrical properties, zinc oxide (ZnO) has received considerable attention from researchers. Even though there is a high demand for functional devices involving ZnO films and nanostructures, synthesizing devices comprising ZnO nanostructures requires expensive and time-consuming techniques that have limited their viability for comprehensive applications. Thus, there is a high demand for high-quality, cost-effective ZnO nanostructures. We have optimized the simple, low-cost, and time-efficient electrochemical deposition (ECD) technique to successfully synthesize high-quality vertically aligned ZnO nanorods to bridge this gap between fundamental and applied research. Characterization of their structural properties through x-ray diffraction and …

Patient Safety Culture, Compassion Fatigue, Compassion Satisfaction: Impact On Nurse-Sensitive Patient Outcomes, Debbie Anglade

Dissertations

The Institute of Medicine (IOM) defines patient safety as "freedom from accidental injury and ensuring patient safety involves establishing operational systems and processes that minimize the possibility of errors and maximize the possibility of intercepting them when they occur". For over a decade healthcare organizations have placed a great deal of effort into establishing operational systems and processes to minimize errors and achieve a culture of safety in their organization. This effort to improve patient safety has been one of the most highly publicized and critical issues facing the healthcare industry. In 1999 the IOM report To Err Is Human …

Correction: Horne Et Al White Matter Correlates Of Domain-Specific Working Memory, Autumn Horne, Junhua Ding, Tatiana T Schnur, Randi C Martin

Faculty, Staff and Student Publications

In the original publication [...].

Optimization Of The Antifungal Properties Of The Bacterial Peptide Entv By Variant Analysis, Shantanu Guha, Shane A Cristy, Giuseppe Buda De Cesare, Melissa R Cruz, Michael C Lorenz, Danielle A Garsin

Faculty, Staff and Student Publications

Fungal resistance to commonly used medicines is a growing public health threat, and there is a dire need to develop new classes of antifungals. We previously described a peptide produced by Enterococcus faecalis, EntV, that restricts Candida albicans to a benign form rather than having direct fungicidal activity. Moreover, we showed that one 12-amino acid (aa) alpha helix of this peptide retained full activity, with partial activity down to the 10aa alpha helix. Using these peptides as a starting point, the current investigation sought to identify the critical features necessary for antifungal activity and to screen for new variants …

Primate Social Behavior And Embodied Action, Maria Elbon

Master's Theses

The evolutionary origins of uniquely human cognitive processes remain poorly understood. Comparative analysis between humans and great apes can help identify derived characteristics and extrapolate ecological conditions leading to the evolution of specifically human cognitive abilities. It has been posited that the relatively large brain size of primates, in relation to body size, evolved in response to increasing social complexity and the accompanying cognitive demands placed on gregarious organisms that need to maintain cohesive, functional groups. Studies examining primate social behavior and cognition have focused on foundational abilities and cognitive capacities that are theorized to act as progenitors to uniquely …

Structured Light Radial-View Endoscope Imaging Development For Evaluation Of Human Tissue, Xiyan Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

In this thesis, we improved an existing structured light methodology to more accurately estimate tissue optical properties imaged in a single snapshot. The developed approach is a major enhancement of the traditional three-phase spatial frequency domain imaging system: It greatly simplifies the operational procedure while also maintaining high accuracy in optical property measurements .

Leveraging this improved approach, we have developed a low-cost, compact, and radial-view endoscope prototype. This prototype combines affordability with high functionality and a user-friendly design. To assess the performance and reliability of our endoscope, we conducted comprehensive evaluations involving tissue-mimicking phantoms, as well as ex vivo …

Reactivity Of Adsorbed Trace Metals At Metal (Oxyhydr)Oxide Surfaces As Probed With Isotope Exchange, Greg Ledingham

Arts & Sciences Graduate Student Theses and Dissertations

Metal (oxyhydr)oxide surfaces regulate the accessibility of trace metals in soils and aquatic systems. Up to half of the trace metals in urban environments, river systems, and contaminated soils are associated with mineral surfaces yet this pool often remains bioaccessible. Past studies of adsorption-desorption suggest that macroscopic rates of adsorption at mineral surfaces are likely mass transfer-limited and show biphasic rates, with a fast-adsorbing fraction equilibrating in seconds to minutes and a slower-adsorbing pool binding over a timescale of hours to days. In addition, adsorption-desorption hysteresis has been observed for some metals after aging at metal (oxyhydr)oxide surfaces, indicating that …

Functions Of Cdc1 In Mediating Cd4+ Help For Cd8+ T Cells, Renee Wu

Arts & Sciences Graduate Student Theses and Dissertations

Classical dendritic cells (cDCs) are specialized antigen presenting cells that comprise distinct subsets distinguished by transcriptional developmental differences and in the types of immune responses they initiate. Classical type 1 dendritic cells (cDC1) are thought to perform antigen cross-presentation, which is required to prime CD8 T cells, whereas cDC2 have been suggested to be specialized for priming CD4 T cells. CD4 T cell help for CD8 T cell responses has been proposed to be mediated by CD40-dependent licensing of antigen-presenting cells. cDC1 have been suggested as the target of CD4 T cell help on the basis of in vitro analysis …

Clearing The Light Path: Proteoglycans And Their Important Roles In The Lens And Cornea, Mary Ann Stepp, A. Sue Menko

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Some of the earliest studies of glycans were performed on mammalian corneas and lenses with many of the key concepts we currently recognize as being fundamental to our understanding of basic cell biology arising from these studies. Proteoglycans and their GAG side chains are essential components of the ECM of the lens capsule. They also are present in the anterior corneal epithelial basement membrane and the posterior (Decemet's) basement membrane, and they organize collagen fiber diameters and spacing in the corneal stroma to maintain stromal clarity. Studies using genetically engineered mice and characterization of spontaneously arising mutations in genes controlling …

Health Care Utilization And Behavior Changes After Workplace Genetic Testing At A Large Us Health Care System, Elizabeth Charnysh, Subhamoy Pal, Jonathan Reader, Wendy R. Uhlmann, Sarah Mccain, Kunal Sanghavi, Drew Blasco, Rachael Brandt, William Gregory Feero, Rebecca Ferber, Veda N. Giri, Katherine Hendy, Anya E. R. Prince, Charles Lee, J. Scott Roberts

College of Life Sciences Faculty Papers

PURPOSE: This study explored employee health behavior changes and health care utilization after workplace genetic testing (wGT). Wellness-program-associated wGT seeks to improve employee health, but the related health implications are unknown.

METHODS: Employees of a large US health care system offering wGT (cancer, heart disease, and pharmacogenomics [PGx]) were sent electronic surveys. Self-reported data from those who received test results were analyzed. Descriptive statistics characterized responses, whereas logistic regression analyses explored correlates of responses to wGT.

RESULTS: 53.9% (n = 418/776) of respondents (88.3% female, mean age = 44 years) reported receiving wGT results. 12.0% (n = 48/399) received results …

Advertising To Generation Z, Taryn Romaine

Honors College Theses

The goal of this project is to analyze the history and background of those born between 1997 and 2012, also known as Generation Z, and determine its effect on how this Generation receives and reacts to advertising. In addition, through the use of a personally created and conducted poll, I gathered data on a portion of Generation Z individuals and their preferences to certain elements of advertisement design. These elements are text content, layout, color, and relatability/authenticity. My project discusses how advertisers and advertisement designers, such as myself, can implement these preferences in order to effectively advertise to Generation Z. …

Goin' Down Swinging: Queer Fury, Mad Green

Graduate School of Art Theses

How can kickboxing uplift a community? How can Queer rage be utilized in community building and artmaking?

As a Queer artist, my work is inspired by my own experiences. Through drawing, printmaking, photography, video, performance, sculpture, and social practice, I dissect my violent upbringing and its lingering threads in my adult life. In this essay, I discuss the two most prominent features of my art practice: Fight and Community. I navigate these ideas through past works, such as a performance piece of me destroying a news article, a short film about institutional homophobia through aliens and immaculate conception, and most …

Role Of Integrase-Pp2a Interaction In Human T-Cell Leukemia Virus Type-1 Replication And Pathogenesis, Shayna Turbin

Senior Honors Papers / Undergraduate Theses

Human T-cell leukemia virus type-1 (HTLV-1) is an oncogenic retrovirus that causes multiple disorders, including adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 retroviral integrase binds to the regulatory B’56γ subunit of the host cell Protein Phosphatase 2A (PP2A). Integrase contains a highly conserved LxxIxE motif that is essential for binding, which increases integration efficiency and facilitates HTLV-1 hijack of host cell machinery. We aim to understand how mutations introduced in the highly conserved binding site can affect viral particle production and infectivity. We transfected 729B human lymphoblastoid cells and 293T cells with mutant and wildtype virus. Mutations L213A, …

Towards A Remote Patient Monitoring Platform For Comprehensive Risk Evaluations For People With Diabetic Foot Ulcers, Gozde Cay, M G Finco, Jason Garcia, Jill L Mcnitt-Gray, David G Armstrong, Bijan Najafi

Center on Aging Staff Publications

Diabetic foot ulcers (DFUs) significantly affect the lives of patients and increase the risk of hospital stays and amputation. We suggest a remote monitoring platform for better DFU care. This system uses digital health metrics (scaled from 0 to 10, where higher scores indicate a greater risk of slow healing) to provide a comprehensive overview through a visual interface. The platform features smart offloading devices that capture behavioral metrics such as offloading adherence, daily steps, and cadence. Coupled with remotely measurable frailty and phenotypic metrics, it offers an in-depth patient profile. Additional demographic data, characteristics of the wound, and clinical …

Exome Sequencing Implicates Ancestry-Related Mendelian Variation At Syne1 In Childhood-Onset Essential Hypertension, Ian Copeland, Edmond Wonkam-Tingang, Monesha Gupta-Malhotra, S Shahrukh Hashmi, Yixing Han, Aarti Jajoo, Nancy J Hall, Paula P Hernandez, Natasha Lie, Dan Liu, Jun Xu, Jill Rosenfeld, Aparna Haldipur, Zelene Desire, Zeynep H Coban-Akdemir, Daryl A Scott, Qing Li, Hsiao-Tuan Chao, Ana M Zaske, James R Lupski, Dianna M Milewicz, Sanjay Shete, Jennifer E Posey, Neil A Hanchard

Faculty, Staff and Student Publications

Childhood-onset essential hypertension (COEH) is an uncommon form of hypertension that manifests in childhood or adolescence and, in the United States, disproportionately affects children of African ancestry. The etiology of COEH is unknown, but its childhood onset, low prevalence, high heritability, and skewed ancestral demography suggest the potential to identify rare genetic variation segregating in a Mendelian manner among affected individuals and thereby implicate genes important to disease pathogenesis. However, no COEH genes have been reported to date. Here, we identify recessive segregation of rare and putatively damaging missense variation in the spectrin domain of spectrin repeat containing nuclear envelope …

Virtual Bodies: Probing Fake Flesh, Emily Elhoffer

MFA in Visual Art

This thesis explores the fluid and often elusive concept of the body as mediated through technology and art, questioning the boundaries between the physical and virtual. By investigating the interactions of cultural ideals, technological mediation, and material experimentation, the research delves into how contemporary art practices can challenge and expand our understanding of embodiment.

Central to this exploration is the use of varied mediums such as sculpture, digital imagery, and installation art to create what I term "virtual bodies"—conceptual entities that exist at the intersection of imagination and material reality. These creations often reflect and critique societal norms regarding beauty, …