Digital Commons Network^™

Response To Comments On The Tolerance To Clostridioides Difficile Spores To Sodium Hypochlorite Disinfection, Humaira Ahmed, Lovleen Tina Joshi

Peninsula Dental School

No abstract provided.

Editorial: Transfusion Medicine And Blood, Volume Ii, Robert W. Maitta

Faculty Scholarship

No abstract provided.

Exploring Microplastic Concentrations And The Sedimentology Of San Diego's Offshore Benthic Environments, Rachel Sarner

Theses

Marine pollution studies are showing infiltration of plastic debris in remote parts of oceans including the deep sea. Microplastics (MPs) are being discovered not only in the ocean’s water column but also benthic sediments reaching thousands of meters in depth. This is the first study to analyze MP concentrations in conjunction with sedimentary characteristics from varying deep-sea environments (coastal, deep ocean, offshore terrace) in the Southern California Continental Borderland (CCB). Our goal for this study was to observe how the concentration and type (morphology, color, size) of microplastics varied with distance from shore, with water depth, and between different submarine …

Nano Biosensing Platform For Infectious Disease Diagnostics, Li Liu

Theses

CRISPR-Cas enabled biosensors offer great potential to be at the forefront of diagnostic medicine. This thesis focuses on the principles of CRISPR-Cas technology and its application to biosensors and virus diagnostics. It also provides a detailed discussion of the different detection methods available at this stage. The breakthrough achievements of CRISPR biosensors in nucleic acid detection are summarized. The CRISPR-Cas nano biosensors exhibit high accuracy, sensitivity, selectivity, and versatility, offering great potential for next-generation diagnostic and point-of-care devices. The prospects and future trends of CRISPR biosensors are also described. From an engineering perspective, this thesis develops and applies several methods …

Ciliary Tip Actin Dynamics Regulate Photoreceptor Outer Segment Integrity, Roly Megaw, Abigail Moye, Zhixian Zhang, Fay Newton, Fraser Mcphie, Laura C Murphy, Lisa Mckie, Feng He, Melissa K Jungnickel, Alex Von Kriegsheim, Peter A Tennant, Chloe Brotherton, Christine Gurniak, Alecia K Gross, Laura M Machesky, Theodore G Wensel, Pleasantine Mill

Faculty, Staff and Students Publications

As signalling organelles, cilia regulate their G protein-coupled receptor content by ectocytosis, a process requiring localised actin dynamics to alter membrane shape. Photoreceptor outer segments comprise an expanse of folded membranes (discs) at the tip of highly-specialised connecting cilia, into which photosensitive GPCRs are concentrated. Discs are shed and remade daily. Defects in this process, due to mutations, cause retinitis pigmentosa (RP). Whilst fundamental for vision, the mechanism of photoreceptor disc generation is poorly understood. Here, we show membrane deformation required for disc genesis is driven by dynamic actin changes in a process akin to ectocytosis. We show RPGR, a …

The Macrophage Landscape Across The Lifespan Of A Human Cardiac Allograft, Xiao Li, Diwakar Turaga, Rich G Li, Chang-Ru Tsai, Julianna N Quinn, Yi Zhao, Ruby Wilson, Katherine Carlson, Jun Wang, Joseph A Spinner, Edward J Hickey, Iki Adachi, James F Martin

Faculty, Staff and Student Publications

Background: Much of our knowledge of organ rejection after transplantation is derived from rodent models.

Methods: We used single-nucleus RNA sequencing to investigate the inflammatory myocardial microenvironment in human pediatric cardiac allografts at different stages after transplantation. We distinguished donor- from recipient-derived cells using naturally occurring genetic variants embedded in single-nucleus RNA sequencing data.

Results: Donor-derived tissue resident macrophages, which accompany the allograft into the recipient, are lost over time after transplantation. In contrast, monocyte-derived macrophages from the recipient populate the heart within days after transplantation and form 2 macrophage populations: recipient MP1 and recipient MP2. Recipient MP2s have cell …

An Unexpected Path For Malat1 In Neurons: Trafficking Out Of The Nucleus For Translation, Bradley W Wright, Jeremy E Wilusz

Faculty, Staff and Students Publications

The Malat1 (metastasis-associated lung adenocarcinoma transcript 1) long noncoding RNA is highly and broadly expressed in mammalian tissues, accumulating in the nucleus where it modulates expression and pre-mRNA processing of many protein-coding genes. In this issue of Genes & Development, Xiao and colleagues (doi:10.1101/gad.351557.124) report that a significant fraction of Malat1 transcripts in cultured mouse neurons are surprisingly exported from the nucleus. These transcripts are packaged with Staufen proteins in RNA granules and traffic down the lengths of neurites. They then can be released in a stimulus-dependent manner to be locally translated into a microprotein that alters neuronal gene …

Targeting The Substrate For Atrial Fibrillation: Jacc Review Topic Of The Week, Mark D Mccauley, Gianluca Iacobellis, Na Li, Stanley Nattel, Jeffrey J Goldberger

Faculty, Staff and Students Publications

The identification of the pulmonary veins as a trigger source for atrial fibrillation (AF) has established pulmonary vein isolation (PVI) as a key target for AF ablation. However, PVI alone does not prevent recurrent AF in many patients, and numerous additional ablation strategies have failed to improve on PVI outcomes. This therapeutic limitation may be due, in part, to a failure to identify and intervene specifically on the pro-fibrillatory substrate within the atria and pulmonary veins. In this review paper, we highlight several emerging approaches with clinical potential that target atrial cardiomyopathy-the underlying anatomic, electrical, and/or autonomic disease affecting the …

Molecular Insights Into The Prototypical Single-Stranded Dna-Binding Protein From E. Coli, Nina J Bonde, Alexander G Kozlov, Michael M Cox, Timothy M Lohman, James L Keck

2020-Current year OA Pubs

The SSB protein of

A Fungal Protein Organizes Both Glycogen And Cell Wall Glucans, Liza Loza, Tamara L Doering

2020-Current year OA Pubs

Glycogen is a glucose storage molecule composed of branched α-1,4-glucan chains, best known as an energy reserve that can be broken down to fuel central metabolism. Because fungal cells have a specialized need for glucose in building cell wall glucans, we investigated whether glycogen is used for this process. For these studies, we focused on the pathogenic yeast

Full Issue: Volume 20, Number 1 - Spring 2024

Jefferson Surgical Solutions

A publication for friends and colleagues of Jefferson’s Department of Surgery

In This Issue

Cover Story Nader Hanna, MD, Named Inaugural Chief of the Division of Surgical Oncology - Page 1

Overview Our Jefferson Bicentennial: Thank You Dr. George McClellan - Page 2

Leadership Jefferson Health Names Enterprise Surgeon Leaders - Page 2

Clinical Integration New Center Offers State-of-the-Art Treatment for Chest Wall Disorders - Page 3

Please Welcome New Faculty - Page 3

Those Who Give Paying it Forward: Grateful Alumna Funds Surgical Pilot Grants - Page 4

News in Brief News in Brief - Page 4

What Does ‘Sex’ Mean To You? The Rainbow Bench Gives Their Opinion. A Discussion On Textualism By Amicus Curiae Briefs Written By Nonprofits In Bostock V. Clayton County, Isabella Rojas

Political Analysis

Isabella Rojas is a recent Magna Cum Laude graduate of Seton Hall University Class of 2023, holding a B.A. in Political Science. She is an incoming J.D. Candidate at Rutgers Law School. Ms. Rojas hopes to pursue a career in legal academia while continuing research in LGBTQ+ scholarship. The objective of her research was to critically analyze the influence of the nonprofit sector on the interpretation of legal text. Her thesis was inspired by her interest in judicial interpretation and the philosophical framework underlying court opinions.

Secularization And Women's Liberation: An Analysis Of Women's Freedoms In The Modern Era, Anna Humphrey

Political Analysis

Anna Humphrey, Seton Hall University class of 2024, is a senior Political Science major with minors in Spanish and Environmental Studies. Anna is a member of the Communications Committee for Adelante Latino/a Student Organization and is a Statistics Tutor at the Math Learning Lab on campus. After graduation, she hopes to work in legislative analysis or lobbying. She is primarily interested in environmental and immigration policy. Her thesis paper was inspired by both Professor Jeffrey Togman's research on the rise of atheism and her passion for women's rights.

Nader Hanna, Md, Named Inaugural Chief Of The Division Of Surgical Oncology

Jefferson Surgical Solutions

In January, Jefferson Health and the Sidney Kimmel Medical College, in collaboration with the Sidney Kimmel Cancer Center, named Nader Hanna, MD, as the inaugural chief of the Division of Surgical Oncology and Vice Chair for Cancer Services in the Department of Surgery. Dr. Hanna is also serving as the Enterprise Surgeon Leader for Surgical Oncology (see article on page 2).

The newly created division shares faculty members and advanced practice clinicians with other divisions covering complex general surgical oncology and breast surgery, as well as melanoma, gastrointestinal, colorectal, peritoneal and endocrine surgical oncology specialties, among others.

In his new …

Ankle Mobility And Balance In A Healthy College-Aged Population: A Correlational Study, Anna G. Johnson, Madelyn M. Roiger, Elisa M. Stewart

Applied Health Sciences Student Works

A review of current literature shows a relationship between ankle mobility and balance in an older population, with limited evidence in younger, healthy populations. To bridge this gap, this study aims to explore this relationship in a healthy, college-aged population. Maintaining upright balance is an integrated effort by the vestibular, somatosensory, and visual systems. Regaining a loss of balance happens with three strategies: an ankle strategy for small perturbations, a hip strategy for moderate perturbations, and a stepping strategy for the largest loss to regain a new base of support. Taking these sequential responses into account, the investigators specifically looked …

Conference Speaker Bios, Dusty Layton, Angela Williams

Conference Documents

No abstract provided.

Commencement Program [Spring 2024], Clark University

Commencement Programs

Annual Commencement Program for graduation ceremonies at Clark University, held on May 20th, 2024.

Shielding International Students From Foreign Influence: The Vital Role Of Robust University Research Security Programs, Susan Martinis

Conference Presentations

No abstract provided.

Comprehensive In Vitro And In Vivo Evaluation Of Therapeutic Potential Of Bacopa-Derived Asiatic Acid Against A Human Oral Pathogen Streptococcus Mutans, Rajendran Jeyasri, Pandiyan Muthuramalingam, Arumugam Priya, Rajaiah Alexpandi, N. R. Siva Shanmugam, Saminathan Nivetha, Hyunsuk Shin, Shunmugiah Karutha Pandian, Arumugam Veera Ravi, Manikandan Ramesh

Food for Health: Publications

Dental caries is a common human oral disease worldwide, caused by an acid-producing bacteria Streptococcus mutans. The use of synthetic drugs and antibiotics to prevent dental caries has been increasing, but this can lead to severe side effects. To solve this issue, developing and developed countries have resorted to herbal medicines as an alternative to synthetic drugs for the treatment and prevention of dental caries. Therefore, there is an urgent need for plant-derived products to treat such diseases. Bacopa monnieri, a well-documented medicinal plant, contains 52 phytocompounds, including the pentacyclic triterpenoid metabolite known as asiatic acid (ASTA). Hence, …

Taming The "Dartmouth Animal", Kira Lynn Parrish-Penny

Sociology Undergraduate Senior Theses

Reflecting on Dartmouth College’s tumultuous early years of coeducation in the 1970s, former president John Kemeny remarked that several students “took it on as a sort of mission to make life miserable for women students when they first arrived… from verbal abuse to … raids on women’s dormitories and… doing everything possible that a minority of students can do to make the women feel unwelcome.” Using archival data from the Rauner Special Collections Library, this thesis explores two questions: (1) how were norms and values around masculinity constructed at Dartmouth? (2) How did this construction of masculinity shift over the …

Initiating Access To Palliative Care Earlier In Pediatric Oncology Patients, Anita Sharma

Doctor of Nursing Practice Final Manuscripts

Abstract

Background: Pediatric oncology patients should be referred to palliative care early but despite research indicating the clear benefits, there is still a delay in referring patients. One of the reasons include lack of understanding amongst providers on what palliative care is. Palliative care provides symptom management, emotional support and helps the patient and family with difficult decision making.

Objectives: This project aimed to increase the number of early referrals to palliative care. The Pediatric Palliative Care Screening Tool (PaPas) was utilized to help identify the triggers which should prompt the referral.

Methods: A chart review of patients diagnosed with …

Synthesis And Regioselective Functionalization Of Tetrafluorobenzo-[Α]-Fused Bopypy Dyes, Sebastian O. Oloo, Guanyu Zhang, Petia Bobadova-Parvanova, Seleen Al Horani, Masa Al Horani, Frank R. Fronczek, Kevin M. Smith, Maria Da Graça H. Vicente

Faculty Publications

The synthesis of a new bis-BF2 tetrafluorobenzo-[α]-fused BOPYPY dye from 4,5,6,7-tetrafluoroisoindole and 2-hydrazinopyrazine is reported. The regioselectivity of nucleophilic substitution reactions at the periphery of the tetrafluorinated BOPYPY and its α-bromo derivative were investigated using N-, O-, S-, and C-based nucleophiles. Among the aromatic fluorine atoms, the F² atom is consistently regioselectively substituted, except when the α-position contains a thiophenol group; in this case, F⁴ is substituted instead due to stabilizing π-π-stacking between the two aromatic groups. The α-bromo BOPYPY derivative also reacts under Stille cross-coupling reaction conditions to produce the corresponding α-substituted product. The spectroscopic properties of …

Perspective—Surface-Display Techniques In Electrochemical Biosensor Designs For Health Monitoring, Courtney J. Weber, Megan D. Whisonant, Olivia M. Clay, Olja Simoska

Faculty Publications

Enzymatic and microbial electrochemical biosensors integrate enzymes and microorganisms as biological recognition elements into the sensor design and functionality. Enzyme-based sensors offer high sensitivity and selectivity for target analyte detection. However, these have limited stability necessary for continuous analyte monitoring. Contrarily, microbe-based electrochemical sensors provide a means for continuous analyte sensing but are associated with challenges related to analyte selectivity in complex samples. To address these limitations, surface-display methods, which bind enzymes to microbial surfaces, enhance biosensor selectivity and sensitivity. This perspective outlines the application of surface-display techniques, offering a promising avenue for health monitoring.

‘Mary Gardens’ Bring Catholic Piety To The Garden, Kayla Harris, Bridget Retzloff

Marian Library Faculty Publications

Each spring, some Catholics plant flowers around a statue of the Virgin Mary, reviving a tradition that goes back to medieval England when holy figures were remembered and shared through plant and flower names.

In Mary gardens, plants and flowers have specific Marian names or religious names in addition to their botanical and common names. For example, the plant today known as foxglove or Digitalis purpurea was once known as “Our Lady’s Glove.” Mary gardens also usually include a statue or image of Mary, sometimes holding the infant Jesus, as a focal point for devotion.

As librarians at the University …

A Multi-Material Platform For Imaging Of Single Cell-Cell Junctions Under Tensile Load Fabricated With Two-Photon Polymerization, Jordan Rosenbohm, Grayson Minnick, Bahareh Tajvidi Safa, Amir M. Esfahani, Xiaowei Jin, Haiwei Zhai, Nickolay V. Lavrik, Ruiguo Yang

Department of Mechanical and Materials Engineering: Faculty Publications

We previously reported a single-cell adhesion micro tensile tester (SCAμTT) fabricated from IP-S photoresin with two-photon polymerization (TPP) for investigating the mechanics of a single cell-cell junction under defined tensile loading. A major limitation of the platform is the autofluorescence of IP-S, the photoresin for TPP fabrication, which significantly increases background signal and makes fluorescent imaging of stretched cells difficult. In this study, we report the design and fabrication of a new SCAμTT platform that mitigates autofluorescence and demonstrate its capability in imaging a single cell pair as its mutual junction is stretched. By employing a two-material design using IP-S …

Joint Control Of A Flying Robot And A Ground Vehicle Using Leader-Follower Paradigm, Ayşen Süheyla Bağbaşi, Ali Emre Turgut, Kutluk Bilge Arikan

Turkish Journal of Electrical Engineering and Computer Sciences

In this study, a novel control framework for the collaboration of an aerial robot and a ground vehicle that is connected via a taut tether is proposed. The framework is based on a leader-follower paradigm. The leader follows a desired trajectory while the motion of the follower is controlled by an admittance controller using an extended state observer to estimate the tether force. Additionally, a velocity estimator is also incorporated to accurately assess the leader’s velocity. An essential feature of our system is its adaptability, enabling role switching between the robots when needed. Furthermore, the synchronization performance of the robots …

Systemic Inflammatory Markers Of Persistent Cerebral Edema After Aneurysmal Subarachnoid Hemorrhage, Nadia Khan, Magdalena Kurnik-Łucka, Gniewomir Latacz, Krzysztof Gil

Faculty, Staff and Student Publications

In the first part of this article, the role of intestinal epithelial tight junctions (TJs), together with gastrointestinal dopaminergic and renin-angiotensin systems, are narratively reviewed to provide sufficient background. In the second part, the current experimental data on the interplay between gastrointestinal (GI) dopaminergic and renin-angiotensin systems in the regulation of intestinal epithelial permeability are reviewed in a systematic manner using the PRISMA methodology. Experimental data confirmed the copresence of DOPA decarboxylase (DDC) and angiotensin converting enzyme 2 (ACE2) in human and rodent enterocytes. The intestinal barrier structure and integrity can be altered by angiotensin (1-7) and dopamine (DA). Both …

Revealing Microbial Community Composition And Disinfection Efficacy Evaluation In Wholesale Pork Markets Through 16s Rdna Sequencing, Miao He, Lingyan Ma, Wentao Lv, Yingping Xiao, Hua Yang, Xiofan Wang, Jiangchao Zhao, Hangzhen Lan, Wen Wang, Xingning Xiao

Animal Science Faculty Publications and Presentations

This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p <  .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p <  .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection …

Living On The Outskirts Of Things: Women And Nonbinary People Of Color With Cognitive Disabilities Describe Their Employment Experiences, Laura Spura Rodriguez

Dissertations and Theses

This study examines the experiences of discrimination and the strategies used to navigate employment for women of color and nonbinary people of color (POC) with cognitive disabilities (CD) through their own narratives. Using Dis/ability Critical Race Theory and an interpretive description methodology, this study engaged 10 women of color and/or nonbinary POC with CD in semi-structured interviews in order to answer the following research questions: 1) What stories do women of color and nonbinary POC with CD tell about their experiences seeking employment? 2) What are the narratives of women and nonbinary POC with CD about their experiences of racism, …

Applying Intensification And Process Integration To Accelerate Development And Scale-Up Of Vaccine Production, Mathias Garny

Vaccine Technology IX

Please click Additional Files below to see the full abstract