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Articles 70291 - 70320 of 820159
Full-Text Articles in Entire DC Network
Manufacturing Of Aligned Electrospun Nanofiber Yarns For Their Enhanced Mechanical And Piezoelectric Properties, Adaugo Mary-Frances Enuka
Manufacturing Of Aligned Electrospun Nanofiber Yarns For Their Enhanced Mechanical And Piezoelectric Properties, Adaugo Mary-Frances Enuka
Theses and Dissertations
Electrospinning is a widely used technique for producing nanofibers with diverse applications. This method uses polymer solutions and strong electric fields to generate nano-sized fibers with unique properties. This study presents a novel manufacturing approach that enables the production of electrospun poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) nanofiber yarns using an automated parallel track system and an adjustable roll-to-roll collector. This research examines the impact of an automated post-drawing system on the polymer chain orientation, crystallinity, tensile strength, morphology, and piezoelectric properties of PVDF-HFP nanofiber yarn. The results reveal that the post-drawing process significantly improves molecular alignment, increases tensile strength, and enhances the …
The Fate Of Fixed Nitrogen In Santa Barbara Basin Sediments During Seasonal Anoxia, Xuefeng Peng, David J. Yousavich, Annie Bourbonnais, Frank Wenzhöfer, Felix Janssen, Tina Treude, David L. Valentine
The Fate Of Fixed Nitrogen In Santa Barbara Basin Sediments During Seasonal Anoxia, Xuefeng Peng, David J. Yousavich, Annie Bourbonnais, Frank Wenzhöfer, Felix Janssen, Tina Treude, David L. Valentine
Faculty Publications
Despite long-standing interest in the biogeochemistry of the Santa Barbara Basin (SBB), there are no direct rate measurements of different nitrogen transformation processes. We investigated benthic nitrogen cycling using in situ incubations with 15NO 3̅ addition and quantified the rates of total nitrate (NO 3̅) uptake, denitrification, anaerobic ammonia oxidation (anammox), N2O production, and dissimilatory nitrate reduction to ammonia (DNRA). Denitrification was the dominant NO 3̅ reduction process, while anammox contributed 0 %–27 % to total NO 3̅ reduction. DNRA accounted for less than half of NO 3̅ reduction except at the …
Loss Of Hormone Receptor Expression After Exposure To Fluid Shear Stress In Breast Cancer Cell Lines, Jonathan Cuccia, Braulio Andres Ortega Quesada, Ethan P. Littlefield, Alejandra M. Ham, Matthew E. Burow, Adam T. Melvin, Elizabeth C. Martin
Loss Of Hormone Receptor Expression After Exposure To Fluid Shear Stress In Breast Cancer Cell Lines, Jonathan Cuccia, Braulio Andres Ortega Quesada, Ethan P. Littlefield, Alejandra M. Ham, Matthew E. Burow, Adam T. Melvin, Elizabeth C. Martin
School of Medicine Faculty Publications
Following metastatic spread, many hormone receptor positive (HR(+)) patients develop a more aggressive phenotype with an observed loss of the HRs estrogen receptor (ER) and progesterone receptor (PR). During metastasis, breast cancer cells are exposed to high magnitudes of fluid shear stress (FSS). Unfortunately, the role for FSS on the regulation of HR expression and function during metastasis is not fully understood. This study was designed to elucidate the impact of FSS on HR(+) breast cancer. Utilizing a microfluidic platform capable of exposing breast cancer cells to FSS that mimics in situ conditions, we demonstrate the impact of FSS exposure …
Mhealth-Based Just-In-Time Adaptive Intervention To Improve The Physical Activity Levels Of Individuals With Spinal Cord Injury: Protocol For A Randomized Controlled Trial, Rachel Carey, Ha Le, Donna Coffman, Inbal Nahum-Shani, Mohanraj Thirumalai, Cole Hagen, Laura Baehr, M. Schmidt-Read, Marlyn Lamboy, Stephanie A Kolakowsky-Hayner, Ralph Marino, Stephen Intille, Shivayogi Hiremath
Mhealth-Based Just-In-Time Adaptive Intervention To Improve The Physical Activity Levels Of Individuals With Spinal Cord Injury: Protocol For A Randomized Controlled Trial, Rachel Carey, Ha Le, Donna Coffman, Inbal Nahum-Shani, Mohanraj Thirumalai, Cole Hagen, Laura Baehr, M. Schmidt-Read, Marlyn Lamboy, Stephanie A Kolakowsky-Hayner, Ralph Marino, Stephen Intille, Shivayogi Hiremath
Moss-Magee Rehabilitation Papers
BACKGROUND: The lack of regular physical activity (PA) in individuals with spinal cord injury (SCI) in the United States is an ongoing health crisis. Regular PA and exercise-based interventions have been linked with improved outcomes and healthier lifestyles among those with SCI. Providing people with an accurate estimate of their everyday PA level can promote PA. Furthermore, PA tracking can be combined with mobile health technology such as smartphones and smartwatches to provide a just-in-time adaptive intervention (JITAI) for individuals with SCI as they go about everyday life. A JITAI can prompt an individual to set a PA goal or …
Chemotherapy Drug Release From Hollow Core Polyethyleneimine Nanoparticles, Brian Boyle
Chemotherapy Drug Release From Hollow Core Polyethyleneimine Nanoparticles, Brian Boyle
Theses and Dissertations
Novel anticancer delivery vehicles are arising from engineered nanostructured materials. This work involved the synthesis, characterization, and release of novel hollow core polymeric nanoparticles (HCPNPs) for controlled drug delivery. Polyethyleneimine (PEI) was bound to AuNPs, forming polymer-shell nanoparticles with dissolution of the gold core via iodine forming HCPNPs with drug intercalated DNA bound to the surface. A high density (85 molecules/particle) of DNA intercalated with daunorubicin was conjugated. Particles were spherical with an average diameter of 105.7 ± 17.3 nm and zeta potential of 20.4 ± 3.54 mV. We hypothesize the DNA backbone electrostatically condensed to the primary amines on …
Python Tooth–Inspired Fixation Device For Enhanced Rotator Cuff Repair, Iden Kurtaliaj, Ethan D. Hoppe, Yuxuan Huang, David Ju, Jacob A. Sandler, Donghwan Yoon, Lester J. Smith, Silvio Torres Betancur, Linda Effiong, Thomas Gardner, Liana Tedesco, Sohil Desai, Victor Birman, William N. Levine
Python Tooth–Inspired Fixation Device For Enhanced Rotator Cuff Repair, Iden Kurtaliaj, Ethan D. Hoppe, Yuxuan Huang, David Ju, Jacob A. Sandler, Donghwan Yoon, Lester J. Smith, Silvio Torres Betancur, Linda Effiong, Thomas Gardner, Liana Tedesco, Sohil Desai, Victor Birman, William N. Levine
Mechanical and Aerospace Engineering Faculty Research & Creative Works
Rotator cuff repair surgeries fail frequently, with 20 to 94% of the 600,000 repairs performed annually in the United States resulting in retearing of the rotator cuff. The most common cause of failure is sutures tearing through tendons at grasping points. To address this issue, we drew inspiration from the specialized teeth of snakes of the Pythonoidea superfamily, which grasp soft tissues without tearing. To apply this nondamaging gripping approach to the surgical repair of tendon, we developed and optimized a python tooth–inspired device as an adjunct to current rotator cuff suture repair and found that it nearly doubled repair …
Teaching Bioinformatics Through The Analysis Of Sars-Cov-2: Project-Based Training For Computer Science Students, Pavlin G Poličar, Martin Špendl, Tomaž Curk, Blaž Zupan
Teaching Bioinformatics Through The Analysis Of Sars-Cov-2: Project-Based Training For Computer Science Students, Pavlin G Poličar, Martin Špendl, Tomaž Curk, Blaž Zupan
Faculty, Staff and Students Publications
MOTIVATION: We learn more effectively through experience and reflection than through passive reception of information. Bioinformatics offers an excellent opportunity for project-based learning. Molecular data are abundant and accessible in open repositories, and important concepts in biology can be rediscovered by reanalyzing the data.
RESULTS: In the manuscript, we report on five hands-on assignments we designed for master's computer science students to train them in bioinformatics for genomics. These assignments are the cornerstones of our introductory bioinformatics course and are centered around the study of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). They assume no prior knowledge of molecular …
The Application Of Multi-Isotope Analysis To Assist In Geoprofiling Unidentified Decedents Within Florida, Liotta D. Noche-Dowdy
The Application Of Multi-Isotope Analysis To Assist In Geoprofiling Unidentified Decedents Within Florida, Liotta D. Noche-Dowdy
USF Tampa Graduate Theses and Dissertations
The biochemistry of human tissues can provide information for geoprofiling (geographic birthplace or migration patterns) of an unidentified decedent. Utilizing isotope analyses (both stable and radiogenic) from teeth, bone, nails, and/or hair reveals specific isotopic information that correlates to diverse geographic regions reflecting an individual’s birthplace and migration patterns. Isotope analysis of enamel and bone has been employed in bioarchaeology for decades to reconstruct the diet, health, and migration patterns of past populations. Isotope ratios measured from enamel can then be compared to isotope ratios measured from bone of the individual to track patterns of migration. The enamel from the …
Physiological And Circadian Outcomes Of Fkbp5 Imbalance: Insights From Two Models, Niat Tsegai Gebru
Physiological And Circadian Outcomes Of Fkbp5 Imbalance: Insights From Two Models, Niat Tsegai Gebru
USF Tampa Graduate Theses and Dissertations
Circadian rhythms are crucial in regulating various physiological processes, including mood regulation, and disruptions in these rhythms have been linked to several neuropsychiatric disorders, such as depression, bipolar disorder, and schizophrenia. FKBP51, a protein involved in stress responses and glucocorticoid receptor function has emerged as a potential regulator of the circadian clock. FKBP51’s interaction with glucocorticoid receptors impacts the transcriptional regulation of circadian-related genes. In recent years, FKBP51 has been associated with genetic and epigenetic alterations in neuropsychiatric disorders, highlighting its potential as a target for intervention. The heterogeneity associated with neuropsychiatric disorders arises from a combination of genetic, environmental, …
Cutaneous Wound Healing And The Effects Of Cannabidiol, Pearl Shah, Kathryne Holmes, Fairouz Chibane, Phillip Wang, Pablo Chagas, Evila Salles, Melanie Jones, Patrick Palines, Mohamad Masoumy, Babak Baban, Jack Yu
Cutaneous Wound Healing And The Effects Of Cannabidiol, Pearl Shah, Kathryne Holmes, Fairouz Chibane, Phillip Wang, Pablo Chagas, Evila Salles, Melanie Jones, Patrick Palines, Mohamad Masoumy, Babak Baban, Jack Yu
School of Medicine Faculty Publications
Cutaneous wounds, both acute and chronic, begin with loss of the integrity, and thus barrier function, of the skin. Surgery and trauma produce acute wounds. There are 22 million surgical procedures per year in the United States alone, based on data from the American College of Surgeons, resulting in a prevalence of 6.67%. Acute traumatic wounds requiring repair total 8 million per year, 2.42% or 24.2 per 1000. The cost of wound care is increasing; it approached USD 100 billion for just Medicare in 2018. This burden for wound care will continue to rise with population aging, the increase in …
The Cns Relapse In T-Cell Lymphoma Index Predicts Cns Relapse In Patients With T- And Nk-Cell Lymphomas, Rahul S. Bhansali, Fredrik Ellin, Thomas Relander, Miao Cao, Wenrui Li, Qi Long, Nivetha Ganesan, Robert Stuver, Steven M. Horwitz, Kitsada Wudhikarn, Steven R. Hwang, N. Nora Bennani, Julio Chavez, Lubomir Sokol, Hayder Saeed, Frank Duan, Pierluigi Porcu, Priyanka Pullarkat, Neha Mehta-Shah, Jasmine M. Zain, Miguel Ruiz, Jonathan E. Brammer, Rishab Prakash, Swaminathan P. Iyer, Adam J. Olszewski, Ajay Major, Peter A. Riedell, Sonali M. Smith, Caroline Goldin, Bradley Haverkos, Bei Hu, Tony Z. Zhuang, Pamela B. Allen, Wael Toama, Murali Janakiram, Taylor R. Brooks, Deepa Jagadeesh, Nisha Hariharan, Aaron M. Goodman, Gabrielle Hartman, Paola Ghione, Fatima Fayyaz, Joanna M. Rhodes, Elise A. Chong, James N. Gerson, Daniel J. Landsburg, Sunita D. Nasta, Stephen J. Schuster, Jakub Svoboda, Mats Jerkeman, Stefan K. Barta
The Cns Relapse In T-Cell Lymphoma Index Predicts Cns Relapse In Patients With T- And Nk-Cell Lymphomas, Rahul S. Bhansali, Fredrik Ellin, Thomas Relander, Miao Cao, Wenrui Li, Qi Long, Nivetha Ganesan, Robert Stuver, Steven M. Horwitz, Kitsada Wudhikarn, Steven R. Hwang, N. Nora Bennani, Julio Chavez, Lubomir Sokol, Hayder Saeed, Frank Duan, Pierluigi Porcu, Priyanka Pullarkat, Neha Mehta-Shah, Jasmine M. Zain, Miguel Ruiz, Jonathan E. Brammer, Rishab Prakash, Swaminathan P. Iyer, Adam J. Olszewski, Ajay Major, Peter A. Riedell, Sonali M. Smith, Caroline Goldin, Bradley Haverkos, Bei Hu, Tony Z. Zhuang, Pamela B. Allen, Wael Toama, Murali Janakiram, Taylor R. Brooks, Deepa Jagadeesh, Nisha Hariharan, Aaron M. Goodman, Gabrielle Hartman, Paola Ghione, Fatima Fayyaz, Joanna M. Rhodes, Elise A. Chong, James N. Gerson, Daniel J. Landsburg, Sunita D. Nasta, Stephen J. Schuster, Jakub Svoboda, Mats Jerkeman, Stefan K. Barta
Department of Medical Oncology Faculty Papers
Little is known about risk factors for central nervous system (CNS) relapse in mature T-cell and natural killer cell neoplasms (MTNKNs). We aimed to describe the clinical epidemiology of CNS relapse in patients with MTNKN and developed the CNS relapse In T-cell lymphoma Index (CITI) to predict patients at the highest risk of CNS relapse. We reviewed data from 135 patients with MTNKN and CNS relapse from 19 North American institutions. After exclusion of leukemic and most cutaneous forms of MTNKNs, patients were pooled with non-CNS relapse control patients from a single institution to create a CNS relapse-enriched training set. …
The Discovery Of First Ret Protac With In Vivo Activity, Yafeng Wang
The Discovery Of First Ret Protac With In Vivo Activity, Yafeng Wang
USF Tampa Graduate Theses and Dissertations
This report mainly discusses the development of RET Proteolysis targeting chimera PROTAC molecules based on Selpercatinib (LOXO-292) and Sulfono-γ-AApeptide PROTAC. A PROTAC molecule is a bifunctional molecule composed of two active domains and a linker. It works by inducing selective protein intracellular degradation. This technology was first discussed by Kathleen Sakamoto, Ray Deshaies and Craig Crews in 2001. Using various E3 Ligase, such as CRBN, pVHL, beta-TrCP1, Mdm2, DCAF15, DCAF16, RNF114, and c-IAP1, the PROTAC technology has been applied to several novel drug development,. Yale University licensed the PROTAC technology to Arvinas in 2013–14. For my research, Chapter one summarizes …
3d Organ-Scale Models Of Tumor Growth And Treatment, Rafael Ramon Bravo
3d Organ-Scale Models Of Tumor Growth And Treatment, Rafael Ramon Bravo
USF Tampa Graduate Theses and Dissertations
To understand the dynamics of cancer, mathematical oncologists have developed models of tumor growth and treatment response. Some models are mechanistic and approach tumor growth at the cell-scale, focusing on the evolution of cancerous cells within the ecology of normal tissue, and are often simulated with agent-based modeling. Other models are more clinically motivated and model tumor growth operating at the organ-scale, using patient data to predict treatment response, and are often simulated with partial differential equations. We developed the Hybrid Automata Library which includes both agent-based modeling and partial differential equations for modeling at either of these scales. We …
The Influence Of Recycled Water On Microbial Source Tracking Techniques That Aim To Identify Untreated Sewage In Surface Waters, Aldo E. Lobos
The Influence Of Recycled Water On Microbial Source Tracking Techniques That Aim To Identify Untreated Sewage In Surface Waters, Aldo E. Lobos
USF Tampa Graduate Theses and Dissertations
Fecal pollution in recreational waters can introduce pathogens that cause increased human health risk, where exposure can occur during recreational activities such as swimming, diving, surfing, and fishing. Furthermore, human fecal pollution (i.e., sewage) can introduce nutrients and other harmful chemicals that may negatively affect the environment and aquatic species. Generally, sewage is considered the highest risk to human health compared to animal fecal pollution because it typically contains a high diversity of pathogens (including human specific viruses), antibiotic resistant bacteria and genes associated with antibiotic resistance. However, pathogens are difficult to detect due to their low concentrations in the …
Site-Specific Conjugation Of Antibodies With Peptides Mediated By Microbial Transglutaminase ( Mtg ) For Adsorption To Gold Nanoparticles, Kwame Osei
Theses and Dissertations
Developing innovative methods that leverage the distinct characteristics of gold nanoparticles (AuNPs) has the potential to significantly advance detection technologies for addressing biological threats and improving disease intervention. However, there are challenges associated with anchoring selective recognition elements, such as antibodies, onto the surface of AuNPs. Despite extensive research, current conjugation approaches for antibody-AuNPs are not universally applicable to all antibodies, are pH-sensitive, result in random orientations that reduce activity, and/or exhibit limited stability. Resolving these issues is crucial to realizing the full potential of AuNP-based assays and promoting their widespread adoption and implementation in the field of diagnostics. The …
Type I Conventional Dendritic Cells Facilitate Immunotherapy In Pancreatic Cancer, Krishnan K Mahadevan, Allison M Dyevoich, Yang Chen, Bingrui Li, Hikaru Sugimoto, Amari M Sockwell, Kathleen M Mcandrews, Lakshmi Kavitha Sthanam, Huamin Wang, Shabnam Shalapour, Stephanie S Watowich, Raghu Kalluri
Type I Conventional Dendritic Cells Facilitate Immunotherapy In Pancreatic Cancer, Krishnan K Mahadevan, Allison M Dyevoich, Yang Chen, Bingrui Li, Hikaru Sugimoto, Amari M Sockwell, Kathleen M Mcandrews, Lakshmi Kavitha Sthanam, Huamin Wang, Shabnam Shalapour, Stephanie S Watowich, Raghu Kalluri
Faculty, Staff and Student Publications
Inflammation and tissue damage associated with pancreatitis can precede or occur concurrently with pancreatic ductal adenocarcinoma (PDAC). We demonstrate that in PDAC coupled with pancreatitis (ptPDAC), antigen-presenting type I conventional dendritic cells (cDC1s) are specifically activated. Immune checkpoint blockade therapy (iCBT) leads to cytotoxic CD8+ T cell activation and elimination of ptPDAC with restoration of life span even upon PDAC rechallenge. Using PDAC antigen-loaded cDC1s as a vaccine, immunotherapy-resistant PDAC was rendered sensitive to iCBT with elimination of tumors. cDC1 vaccination coupled with iCBT identified specific CDR3 sequences in the tumor-infiltrating CD8+ T cells with potential therapeutic importance. This study …
Tuning Polymer Glass Formation Behavior And Understanding Time-Temperature Superposition Breakdown With Oligomeric Additives, Peijing Yue
USF Tampa Graduate Theses and Dissertations
The glass transition of polymer melts has been investigated for over a century. Even so, there are still many unanswered questions about the underlying physics. Countless theories were proposed to explain and define glass transition temperatures. More specifically, both the origins of the glass transition and underlying classification of glass as a state of matter remain unresolved. Time-temperature superposition (TTS) is one of the most widely used techniques to characterize the viscoelastic behavior of a material and the mechanical behavior and is employed to reduce the measuring time during characterization. Despite the widespread use of TTS, many authors have reported …
Aging- And Alcohol-Associated Spatial Transcriptomic Signature In Mouse Acute Pancreatitis Reveals Heterogeneity Of Inflammation And Potential Pathogenic Factors, Rachel R Tindall, Yuntao Yang, Isabella Hernandez, Amy Qin, Jiajing Li, Yinjie Zhang, Thomas H Gomez, Mamoun Younes, Qiang Shen, Jennifer M Bailey-Lundberg, Zhongming Zhao, Daniel Kraushaar, Patricia Castro, Yanna Cao, W Jim Zheng, Tien C Ko
Aging- And Alcohol-Associated Spatial Transcriptomic Signature In Mouse Acute Pancreatitis Reveals Heterogeneity Of Inflammation And Potential Pathogenic Factors, Rachel R Tindall, Yuntao Yang, Isabella Hernandez, Amy Qin, Jiajing Li, Yinjie Zhang, Thomas H Gomez, Mamoun Younes, Qiang Shen, Jennifer M Bailey-Lundberg, Zhongming Zhao, Daniel Kraushaar, Patricia Castro, Yanna Cao, W Jim Zheng, Tien C Ko
Faculty, Staff and Student Publications
The rapidly aging population is consuming more alcohol, leading to increased alcohol-associated acute pancreatitis (AAP) with high mortality. However, the mechanisms remain undefined, and currently there are no effective therapies available. This study aims to elucidate aging- and alcohol-associated spatial transcriptomic signature by establishing an aging AAP mouse model and applying Visium spatial transcriptomics for understanding of the mechanisms in the context of the pancreatic tissue. Upon alcohol diet feeding and caerulein treatment, aging mice (18 months) developed significantly more severe AAP with 5.0-fold increase of injury score and 2.4-fold increase of amylase compared to young mice (3 months). Via …
Neural Circuits Expressing The Serotonin 2c Receptor Regulate Memory In Mice And Humans, Hesong Liu, Yang He, Hailan Liu, Bas Brouwers, Na Yin, Katherine Lawler, Julia M Keogh, Elana Henning, Dong-Kee Lee, Meng Yu, Longlong Tu, Nan Zhang, Kristine M Conde, Junying Han, Zili Yan, Nikolas A Scarcelli, Lan Liao, Jianming Xu, Qingchun Tong, Hui Zheng, Zheng Sun, Yongjie Yang, Chunmei Wang, Yanlin He, I Sadaf Farooqi, Yong Xu
Neural Circuits Expressing The Serotonin 2c Receptor Regulate Memory In Mice And Humans, Hesong Liu, Yang He, Hailan Liu, Bas Brouwers, Na Yin, Katherine Lawler, Julia M Keogh, Elana Henning, Dong-Kee Lee, Meng Yu, Longlong Tu, Nan Zhang, Kristine M Conde, Junying Han, Zili Yan, Nikolas A Scarcelli, Lan Liao, Jianming Xu, Qingchun Tong, Hui Zheng, Zheng Sun, Yongjie Yang, Chunmei Wang, Yanlin He, I Sadaf Farooqi, Yong Xu
Children’s Nutrition Research Center Staff Publications
Declined memory is a hallmark of Alzheimer’s disease (AD). Experiments in rodents and human postmortem studies suggest that serotonin (5-hydroxytryptamine, 5-HT) plays a role in memory, but the underlying mechanisms are unknown. Here, we investigate the role of 5-HT 2C receptor (5-HT2CR) in regulating memory. Transgenic mice expressing a humanized HTR2C mutation exhibit impaired plasticity of hippocampal ventral CA1 (vCA1) neurons and reduced memory. Further, 5-HT neurons project to and synapse onto vCA1 neurons. Disruption of 5-HT synthesis in vCA1-projecting neurons or deletion of 5-HT2CRs in the vCA1 impairs neural plasticity and memory. We show that a selective 5-HT2CR agonist, …
Obesity Is Associated With Muscle Atrophy In Rotator Cuff Tear, Simone D Herzberg, Robert H Brophy, Matthew V Smith, Et Al.
Obesity Is Associated With Muscle Atrophy In Rotator Cuff Tear, Simone D Herzberg, Robert H Brophy, Matthew V Smith, Et Al.
2020-Current year OA Pubs
OBJECTIVE: The primary goal of this study is to evaluate the relationship between Body Mass Index (BMI) and muscle atrophy in individuals with rotator cuff tears.
METHODS: This study consists of patients with rotator cuff tears identified by MRI from two independent cohorts, the Rotator Cuff Outcomes Workgroup (ROW) and the Multicenter Orthopaedic Outcomes Network (MOON). Presence of atrophy (yes/no) and severity of atrophy (as an ordinal variable) were assessed on MRI by expert physicians. We used multivariable regression models to evaluate the relationship between BMI and muscle atrophy while adjusting for age and sex in each study, conducted sensitivity …
Using Human Intestinal Organoids To Understand The Small Intestine Epithelium At The Single Cell Transcriptional Level, Carolyn Bomidi, Xi-Lei Zeng, Victoria Poplaski, Cristian Coarfa, Mary K Estes, Sarah E Blutt
Using Human Intestinal Organoids To Understand The Small Intestine Epithelium At The Single Cell Transcriptional Level, Carolyn Bomidi, Xi-Lei Zeng, Victoria Poplaski, Cristian Coarfa, Mary K Estes, Sarah E Blutt
Faculty, Staff and Students Publications
Single cell transcriptomics has revolutionized our understanding of the cell biology of the human body. State-of-the-art human small intestinal organoid cultures provide ex vivo model systems that bridge the gap between animal models and clinical studies. The application of single cell transcriptomics to human intestinal organoid (HIO) models is revealing previously unrecognized cell biology, biochemistry, and physiology of the GI tract. The advanced single cell transcriptomics platforms use microfluidic partitioning and barcoding to generate cDNA libraries. These barcoded cDNAs can be easily sequenced by next generation sequencing platforms and used by various visualization tools to generate maps. Here, we describe …
Python Tooth-Inspired Fixation Device For Enhanced Rotator Cuff Repair, Iden Kurtaliaj, Ethan D Hoppe, Yuxuan Huang, David Ju, Jacob A Sandler, Donghwan Yoon, Guy M Genin
Python Tooth-Inspired Fixation Device For Enhanced Rotator Cuff Repair, Iden Kurtaliaj, Ethan D Hoppe, Yuxuan Huang, David Ju, Jacob A Sandler, Donghwan Yoon, Guy M Genin
2020-Current year OA Pubs
Rotator cuff repair surgeries fail frequently, with 20 to 94% of the 600,000 repairs performed annually in the United States resulting in retearing of the rotator cuff. The most common cause of failure is sutures tearing through tendons at grasping points. To address this issue, we drew inspiration from the specialized teeth of snakes of the Pythonoidea superfamily, which grasp soft tissues without tearing. To apply this nondamaging gripping approach to the surgical repair of tendon, we developed and optimized a python tooth-inspired device as an adjunct to current rotator cuff suture repair and found that it nearly doubled repair …
Going Cashless: Privacy Implications For Gun Control In A Digital Economy, Liza Goldenberg
Going Cashless: Privacy Implications For Gun Control In A Digital Economy, Liza Goldenberg
The Journal of Business, Entrepreneurship & the Law
This paper will examine how, given the United States’ shift toward a cashless economy, the country’s top credit-card companies’ potential decision to implement a new merchant code for firearm-related transactions as a method of gun control will backfire, jeopardizing consumer privacy and leading to unregulated transactions through cryptocurrencies. Since the majority of gun violence stems from firearm transactions not involving credit cards, credit-card companies should abandon the new merchant code that dissuades Americans from exercising their fundamental rights. The American economy should focus on slowing the shift toward a digital economy so that federal and state governments can implement legislation …
Productivity And Genotypic Diversity Of Trifolium Ambiguum In The Central Caucasian Mountains, Georgia, R G. Patterson, P R. Espie
Productivity And Genotypic Diversity Of Trifolium Ambiguum In The Central Caucasian Mountains, Georgia, R G. Patterson, P R. Espie
IGC Proceedings (1977-2023)
Trifolium ambiguum occurred in12 diverse grassland communities, ranging from 1700 m to 2850 m, in the Kasbegi region, Central Caucasus. Total herbage mass in mid summer ranged from 310 to 763 g/m2with T. ambiguum composition ranging from 0.5 to 51%. Maximum T. ambiguum production occurred in fertile, regularly mown hay meadows, but was reduced in severely grazed pastures. It has persisted in these communities after centuries of grazing or cutting, without fertiliser input. T. ambiguum ploidy levels were predominantly diploid (82% frequency of occurrence), often in conjunction with triploid (18%) or tetraploid (27%) genotypes. The triploid T. ambiguum genotype ( …
Nitrogen Nutrition Of Brant (Branta Bernicla L.) Grazing On Saltmarsh And Pasture Species, G Q.A Anderson, M Andrews, S M. Percival, J S. Kirby
Nitrogen Nutrition Of Brant (Branta Bernicla L.) Grazing On Saltmarsh And Pasture Species, G Q.A Anderson, M Andrews, S M. Percival, J S. Kirby
IGC Proceedings (1977-2023)
Captive brant Branta bernicla were fed a range of food plants to determine their ability to digest nitrogen and specifically soluble protein. Soluble protein levels in droppings were only a small fraction of those in plants, indicating substantial uptake or conversion. For white clover Trifolium repens L., uric acid content indicated that at least 50% of excreted nitrogen had been metabolised, strongly suggesting that a substantial fraction of soluble protein is utilised by the geese. Carbon/nitrogen ratios increased from plants to droppings while carbon/hydrogen ratios remained nearly constant indicating greater uptake of nitrogen relative to carbon. T. repens was found …
Measuring Spatial Variation Within Pastures, C K. Mcdonald, R M. Jones
Measuring Spatial Variation Within Pastures, C K. Mcdonald, R M. Jones
IGC Proceedings (1977-2023)
Paddock means of pasture yield and composition can be inadequate or even misleading due to the spatial variability within pastures. The use of visual estimation procedures, particularly the BOTANAL technique, provides data suitable for analysing variability as many quadrats are sampled within the one paddock. Examples show how data from routine BOTANAL samplings can reveal small and paddock scale spatial variation, and how BOTANAL data can be used to examine relationships between the yield of legume and grass in a mixed pasture grazed at different stocking rates.
Effect Of Seed Storage On Integrity Of Grass Germplasm, G Zurek
Effect Of Seed Storage On Integrity Of Grass Germplasm, G Zurek
IGC Proceedings (1977-2023)
Experiment on estimation of qualitative changes in populations of meadow fescue (Festuca pratensis Huds.) after seed storage in different conditions were taken. Phenotypical traits for plants derived from seeds stored during 54 months in two different kind of storage conditions were compared. Storage of meadow fescue seed during 54 months in unfavourable conditions resulted in decrease of initial germination as well as in increase of abnormal seedlings and variation. Germination of seed samples stored in cold and dry conditions remain at the initial level. Results of the above experiment prove great variability of tested samples in case of reaction …
Post-Harvest Residue Management For Fine Fescue Seed Crops In Oregon, T G. Chastain, W C. Young Iii, G L. Kiemnec, C J. Garbacik, G A. Gingrich, G H. Cook
Post-Harvest Residue Management For Fine Fescue Seed Crops In Oregon, T G. Chastain, W C. Young Iii, G L. Kiemnec, C J. Garbacik, G A. Gingrich, G H. Cook
IGC Proceedings (1977-2023)
On-farm trials were conducted from 1992 to 1995 to investigate the efficacy of alternative post-harvest residue management practices for production of fine-leaf fescue seed crops, creeping red fescue (Festuca rubra L.) and Chewings fescue (F. rubra L. ssp. commutata Gaud.). Seed yield and seed quality were maintained in Chewings fescue seed crops without burning when residue management techniques removed most of the straw and stubble remaining after harvest. Seed yield was reduced to unacceptable levels by all nonthermal management practices in creeping red fescue.
Proximate Composition Of Mature Seeds From Soybean Plants Grown In Different Environments, T M. Kuo, E Eskins, R L. Cooper
Proximate Composition Of Mature Seeds From Soybean Plants Grown In Different Environments, T M. Kuo, E Eskins, R L. Cooper
IGC Proceedings (1977-2023)
The objectives of this study were to examine the influence of differences in sunlight irradiation on the accumulation of soluble oligosaccharides in mature soybean (Glycine max L.) seeds and to determine the correlations between important seed constituents. Plants were grown in field houses covered with plastic filters that varied sunlight irradiation. The most obvious effect of both blueand red-deficient sunlight was a reduction in the level of stachyose and sucrose that coincided with an increase in raffinose level and decreased oil content. Blue growth house which was deficient in red light spectrum also produced seeds with an increased protein content. …
Management Requirements For Dryland Creeping Bentgrass Seed Production, D J. Cattani, R Gjuric, D E. Feindel, P R. Miller, S R. Smith Jr
Management Requirements For Dryland Creeping Bentgrass Seed Production, D J. Cattani, R Gjuric, D E. Feindel, P R. Miller, S R. Smith Jr
IGC Proceedings (1977-2023)
An extensive research project was initiated in 1992 to develop a dryland seed production system for creeping bentgrass (Agrostis stolonifera L.) in western Canada. Two objectives involved determining the appropriate renovation method and spring nitrogen timing and rate to maximize seed yield. Renovation experiments included scalping, mowing, aeration, tillage, burning and chemical and mechanical gapping and spring N fertilization experiments included at 0, 50, 100, and 150 kg N ha-1 at two dates. Scalping (removal of stubble and growth to 1.25 cm) showed the greatest potential to maintain seed yields. Timing of renovation was also important with early (after …