Digital Commons Network^™

Staying Fresh: Unconventional Approaches Towards Advancing Energy Sustainability, Water Resources, And Community Resiliency In The Southwestern United States, Judith Hoyt

Open Access Theses & Dissertations

This dissertation addresses critical challenges in urban heat management, sustainable energy resource utilization, and water quality communication through three studies. Study 1 investigates the impact of roof color on urban heat islands in Tucson, Arizona where approximately 70% of roofs display high albedo (i.e., light) colors. Energy consumption simulations conducted indicate that converting dark- to light-colored roofs could save Tucson approximately $1,400,000 annually in energy costs, highlighting the potential of cool roofs for energy savings and improved thermal comfort. Study 2 assesses the sources of lithium in subsurface waters in West Texas and South Central New Mexico. Water chemistry data …

Method Development For The Quantification Of Critically Valuable Elements In Permian Basin Produced Waters, Carley Oliver

Open Access Theses & Dissertations

Produced waters (PW) are a major byproduct of the oil and gas industrial hydraulic fracturing processes that create a major waste disposal issue with a need for disposal, treatment, and reuse method developments. The current primary disposal method is subsurface injection, which can lead to high pressures in the subsurface. PWs are often highly saline from mineral leaching due to long residence in subsurface reservoirs. According to the USGS National Minerals Information Center, bromine, calcium chloride, iodine, lithium, magnesium, and sodium chloride have been found in and extracted from these domestic subsurface brines, suggesting that there may be other economically …

Ecological, Climatic, And Geomorphic Controls Of The Transport And Fate Of Co2 In Coastal Arctic Aquatic Ecosystems, Alina Spera

Open Access Theses & Dissertations

Due to the ecological and physical responses of arctic tundra to warming, organic carbon (C) stored in frozen permafrost soils is increasingly at risk of mineralization and export to aquatic systems, the coast, and atmosphere. Although many coastal aquatic systems in the Arctic are likely net sources of CO2 to the atmosphere, little is known about the drivers of inorganic C flux and how these systems contribute to regional C budgets. This dissertation addresses several lapses in our understanding of coastal inorganic C dynamics in arctic Alaska via three data chapters. In chapter 2, I examine seasonal and regional variability …

Neural Networks For Decisions Under Uncertainty, Edwin Tomy George

Open Access Theses & Dissertations

Neural networks are used in many real-world applications, ranging from classification tasks to medical diagnostics. For each task, a neural network is typically able to make predictions due to its ability to extract meaningful patterns from processing large amounts of data. Thus, given the increases in available data in recent decades, the performance of neural networks in making accurate predictions has greatly increased. However, this data often comes with ingrained uncertainties due to measurement errors or the inherent variability of individual data points. Neural networks can learn despite the errors in the overall data, but what if we want them …

Materials Data Science Using Cradle: A Distributed, Data-Centric Approach, Thomas G. Ciardi, Arafath Nihar, Rounak Chawla, Olatunde Akanbi, Pawan K. Tripathi, Yinghui Wu, Vipin Chaudhary, Roger H. French

Faculty Scholarship

There is a paradigm shift towards data-centric AI, where model efficacy relies on quality, unified data. The common research analytics and data lifecycle environment (CRADLE™) is an infrastructure and framework that supports a data-centric paradigm and materials data science at scale through heterogeneous data management, elastic scaling, and accessible interfaces. We demonstrate CRADLE’s capabilities through five materials science studies: phase identification in X-ray diffraction, defect segmentation in X-ray computed tomography, polymer crystallization analysis in atomic force microscopy, feature extraction from additive manufacturing, and geospatial data fusion. CRADLE catalyzes scalable, reproducible insights to transform how data is captured, stored, and analyzed. …

A Mixture-Of-Experts Approach To Fine-Tuning The Segment Anything Model, Rajat Sahay

Theses

The emergence of large, general-purpose foundation models has sparked significant interest in the broader machine learning community. Among the many models being released, the Segment Anything Model (SAM) has demonstrated exceptional capabilities for object segmentation in various settings. Given its expansive training data, SAM has been used for image segmentation across various downstream tasks ranging from tumour segmentation to aerial object detection. However, the majority of pretraining data used by SAM consists of naturally-occurring images, which have significantly different characteristics from images of tumours or images taken by drones. In order to align SAM to these previously unseen domains, we …

Current Trends In Bee Conservation And Habitat Restoration In Different Types Of Anthropogenic Habitats, Olivia Kline, Neelendra K. Joshi

Entomology and Plant Pathology Faculty Publications and Presentations

Recent declines in bee populations and ranges have been cause for concern due to the valuable pollination service that they provide. Several factors have been proposed to contribute to these declines, including habitat loss, pathogen spread, and pesticide usage, so many pollinator conservation schemes have involved the addition of pollinator-friendly habitat through wildflower plantings and artificial nesting sites. Because of this, many efforts have been made to enhance bee populations across different landscape types, including natural, agricultural, urban, and industrial areas. Many of these schemes have focused on providing habitat for bees and other animal pollinators in agricultural landscapes, but …

Utilising Discriminant Function Analysis (Dfa) For Classifying Osteoarthritis (Oa) Patients And Volunteers Based On Biomarker Concentration, Laura Jane Coleman, John L Byrne, Stuart Edwards, Rosemary O'Hara

Faculty, Staff and Student Publications

Osteoarthritis (OA) is a degenerative joint disease characterised by the breakdown of cartilage, causing pain, stiffness, and limited movement. Early diagnosis is crucial for effective management but remains challenging due to non-specific early symptoms. This study explores the application of Discriminant Function Analysis (DFA) to classify OA patients and healthy volunteers based on biomarker concentrations of Interleukin-6 (IL-6), Tumour necrosis factor-alpha (TNF-α), and Myeloperoxidase (MPO). DFA was employed to analyse biomarker data from 86 participants (58 patients, 28 volunteers) to evaluate the discriminatory power of these biomarkers in predicting OA. Significant differences were observed in MPO and TNF-α levels between …

Neoantigen-Specific Cytotoxic Tr1 Cd4 T Cells Suppress Cancer Immunotherapy, Hussein Sultan, Yoshiko Takeuchi, Jeffrey P Ward, Naveen Sharma, Tian-Tian Liu, Vladimir Sukhov, Maria Firulyova, Yuang Song, Samuel Ameh, Simone Brioschi, Darya Khantakova, Cora D Arthur, J Michael White, Heather Kohlmiller, Andres M Salazar, Robert Burns, Helio A Costa, Kelly D Moynihan, Yik Andy Yeung, Ivana Djuretic, Ton N Schumacher, Kathleen C F Sheehan, Marco Colonna, James P Allison, Kenneth M Murphy, Maxim N Artyomov, Robert D Schreiber

Faculty, Staff and Student Publications

CD4+ T cells can either enhance or inhibit tumour immunity. Although regulatory T cells have long been known to impede antitumour responses1-5, other CD4+ T cells have recently been implicated in inhibiting this response6,7. Yet, the nature and function of the latter remain unclear. Here, using vaccines containing MHC class I (MHC-I) neoantigens (neoAgs) and different doses of tumour-derived MHC-II neoAgs, we discovered that whereas the inclusion of vaccines with low doses of MHC-II-restricted peptides (LDVax) promoted tumour rejection, vaccines containing high doses of the same MHC-II neoAgs (HDVax) inhibited rejection. Characterization of the inhibitory cells induced by HDVax identified …

Artificial Intelligence In Fusion Protein Three-Dimensional Structure Prediction: Review And Perspective, Himansu Kumar, Pora Kim

Faculty, Staff and Student Publications

Recent advancements in artificial intelligence (AI) have accelerated the prediction of unknown protein structures. However, accurately predicting the three-dimensional (3D) structures of fusion proteins remains a difficult task because the current AI-based protein structure predictions are focused on the WT proteins rather than on the newly fused proteins in nature. Following the central dogma of biology, fusion proteins are translated from fusion transcripts, which are made by transcribing the fusion genes between two different loci through the chromosomal rearrangements in cancer. Accurately predicting the 3D structures of fusion proteins is important for understanding the functional roles and mechanisms of action …

Poly(Adp-Ribose) Polymerase-1 Regulates Pyroptosis Independent Function Of Nlrp3 Inflammasome In Neutrophil Extracellular Trap Formation, Louis J Delinois, Atul Sharma, Ashwin K Ramesh, Laurel D Boatright, Qun Li, Rong Xu, Hongbo R Luo, Bibhuti B Mishra, Jyotika Sharma

Faculty, Staff and Student Publications

Neutrophil extracellular traps (NETs) function to control infectious agents as well as to propagate inflammatory response in a variety of disease conditions. DNA damage associated with chromatin decondensation and NACHT domain-leucine-rich repeat-and pyrin domain-containing protein 3 (NLRP3) inflammasome activation have emerged as crucial events in NET formation, but the link between the two processes is unknown. In this study, we demonstrate that poly(ADP-ribose) polymerase-1 (PARP-1), a key DNA repair enzyme, regulates NET formation triggered by NLRP3 inflammasome activation in neutrophils. Activation of mouse neutrophils with canonical NLRP3 stimulants LPS and nigericin induced NET formation, which was significantly abrogated by pharmacological …

Mechanism And Rational Combinations With Gp-2250, A Novel Oxathiazine Derivative, In Ovarian Cancer, Mark S Kim, Deanna Glassman, Katelyn F Handley, Adrian Lankenau Ahumada, Nicholas B Jennings, Emine Bayraktar, Katherine Foster, Robiya Joseph, Sanghoon Lee, Robert L Coleman, Anil K Sood

Faculty, Staff and Student Publications

BACKGROUND: GP-2250, a novel analog of taurultam (TRLT), has emerged as a potent anti-neoplastic drug; however, the mechanisms underlying its effects are not well understood. Here, we investigated the mechanism of action and the biological effects of GP-2250 using in vitro and in vivo models.

METHODS: We carried out a series of in vitro (MTT assay, Annexin V/PI assay, colony formation assay, reverse-phase protein array [RPPA], and HRLC/IC analysis) to determine the biological activity of GP-2250 and investigate the mechanism of action. In vivo experiments were carried out to determine the therapeutic efficacy of GP-2250 alone and in combination with …

Autophagic Signaling Promotes Systems-Wide Remodeling In Skeletal Muscle Upon Oncometabolic Stress By D2-Hg, Yaqi Gao, Kyoungmin Kim, Heidi Vitrac, Rebecca L Salazar, Benjamin D Gould, Daniel Soedkamp, Weston Spivia, Koen Raedschelders, An Q Dinh, Anna G Guzman, Lin Tan, Stavros Azinas, David J R Taylor, Walter Schiffer, Daniel Mcnavish, Helen B Burks, Roberta A Gottlieb, Philip L Lorenzi, Blake M Hanson, Jennifer E Van Eyk, Heinrich Taegtmeyer, Anja Karlstaedt

Faculty, Staff and Student Publications

OBJECTIVES: Cachexia is a metabolic disorder and comorbidity with cancer and heart failure. The syndrome impacts more than thirty million people worldwide, accounting for 20% of all cancer deaths. In acute myeloid leukemia, somatic mutations of the metabolic enzyme isocitrate dehydrogenase 1 and 2 cause the production of the oncometabolite D2-hydroxyglutarate (D2-HG). Increased production of D2-HG is associated with heart and skeletal muscle atrophy, but the mechanistic links between metabolic and proteomic remodeling remain poorly understood. Therefore, we assessed how oncometabolic stress by D2-HG activates autophagy and drives skeletal muscle loss.

METHODS: We quantified genomic, metabolomic, and proteomic changes in …

A Biallelic Variant Of The Rna Exosome Gene, Exosc4, Associated With Neurodevelopmental Defects Impairs Rna Exosome Function And Translation, Milo B Fasken, Sara W Leung, Lauryn A Cureton, Maha Al-Awadi, Adila Al-Kindy, Ambro Van Hoof, Sohail Khoshnevis, Homa Ghalei, Almundher Al-Maawali, Anita H Corbett

Faculty, Staff and Student Publications

The RNA exosome is an evolutionarily conserved complex required for both precise RNA processing and decay. Pathogenic variants in EXOSC genes, which encode structural subunits of this complex, are linked to several autosomal recessive disorders. Here, we describe a missense allele of the EXOSC4 gene that causes a collection of clinical features in two affected siblings. This missense variant (NM_019037.3: exon3:c.560T>C) changes a leucine residue within a conserved region of EXOSC4 to proline (p.Leu187Pro). The two affected individuals show prenatal growth restriction, failure to thrive, global developmental delay, intracerebral and basal ganglia calcifications, and kidney failure. Homozygosity for the …

Nf1 Mutation Disrupts Activity-Dependent Oligodendroglial Plasticity And Motor Learning In Mice, Yuan Pan, Jared D Hysinger, Belgin Yalçın, James J Lennon, Youkyeong Gloria Byun, Preethi Raghavan, Nicole F Schindler, Corina Anastasaki, Jit Chatterjee, Lijun Ni, Haojun Xu, Karen Malacon, Samin M Jahan, Alexis E Ivec, Benjamin E Aghoghovwia, Christopher W Mount, Surya Nagaraja, Suzanne Scheaffer, Laura D Attardi, David H Gutmann, Michelle Monje

Faculty, Staff and Student Publications

Neurogenetic disorders, such as neurofibromatosis type 1 (NF1), can cause cognitive and motor impairments, traditionally attributed to intrinsic neuronal defects such as disruption of synaptic function. Activity-regulated oligodendroglial plasticity also contributes to cognitive and motor functions by tuning neural circuit dynamics. However, the relevance of oligodendroglial plasticity to neurological dysfunction in NF1 is unclear. Here we explore the contribution of oligodendrocyte progenitor cells (OPCs) to pathological features of the NF1 syndrome in mice. Both male and female littermates (4-24 weeks of age) were used equally in this study. We demonstrate that mice with global or OPC-specific Nf1 heterozygosity exhibit defects …

Charter Schools Annual Report 2022, Tennessee. Department Of Education.

Charter Schools Annual Report

No abstract provided.

Cerebrovascular Reactivity Mri As A Biomarker For Cerebral Small Vessel Disease–Related Cognitive Decline: Multi-Site Validation In The Markvcid Consortium, Peiying Liu, Zixuan Lin, Kaisha Hazel, George Pottanat, Cuimei Xu, Dengrong Jiang, Jay J. Pillai, Emma Lucke, Christopher E. Bauer, Brian T. Gold, Steven M. Greenburg, Karl G. Helmer, Kay Jann, Gregory Jicha, Joel Kramer, Pauline Maillard, Rachel M. Mulavelil, Pavel Rodriguez, Claudia L. Satizabal, Kristin Schwab, Sudha Seshadri, Herpreet Singh, Ángel G. Velarde Dediós, Danny J. J. Wang, Rita R. Kalyani, Abhay Moghekar, Paul B. Rosenberg, Sevil Yasar, Marilyn Albert, Hanzhang Lu

Neurology Faculty Publications

INTRODUCTION: Vascular contributions to cognitive impairment and dementia (VCID) represent a major factor in cognitive decline in older adults. The present study examined the relationship between cerebrovascular reactivity (CVR) measured by magnetic resonance imaging (MRI) and cognitive function in a multi-site study, using a predefined hypothesis.

METHODS: We conducted the study in a total of three analysis sites and 263 subjects. Each site performed an identical CVR MRI procedure using 5% carbon dioxide inhalation. A global cognitive measure of Montreal Cognitive Assessment (MoCA) and an executive function measure of item response theory (IRT) score were used as outcomes.

RESULTS: CVR …

Performance And Enhanced Efficiency Induced By Cold Plasma On Sapo-34 Membranes For Co2 And Ch4 Mixtures, Fnu Gorky, Vashanti Storr, Grace Jones, Apolo Nambo, Jacek B. Jasinski, Maria L. Carreon

Chemical Engineering Faculty Publications and Presentations

In this study, we investigate the influence of cold-plasma-induced enhanced performance and efficiency of SAPO-34 membranes in the separation of CO₂ and CH₄ mixtures. Placing the herein presented research in a broader context, we aim to address the question of whether cold plasma can significantly impact the membrane performance. We subjected SAPO-34 membranes to plasma mild disturbances and analyzed their performance in separating CO₂ and CH₄. Our findings reveal a notable enhancement in membrane efficiency and sustained performance when exposed to cold plasma. The pulsed plasma separation displayed improved structural integrity, and the experimental results …

An Experimental Investigation Of Printing Speed, Layer Thickness, And Nozzle Temperature On The Mechanical Properties Of Silk-Pla Printed Specimens, Razaul Islam

All Theses

Fused Deposition Modeling (FDM) is a popular 3D printing technique used across various industries. Choosing the right printing parameters is essential for ensuring the overall quality and integrity of 3D-printed products. These parameters greatly affect the quality and strength of 3D-printed items. The focus of this study was the influence of printing parameters such as layer thickness, nozzle temperature, and printing speed on the mechanical behavior of 3D-printed silk polylactic acid (PLA). Tensile tests were conducted on a universal testing machine to analyze the mechanical behavior of the printed materials. Moreover, Digital Image Correlation (DIC) analyses were utilized on the …

Analysis Of The Relationship Between Flow Patterns In The Post-Stenotic Region Of Model Arteries And Stenosis Severity., Michael Laroche

Electronic Theses and Dissertations

In this study, contour fields of mean age and turbulence kinetic energy (TKE) in the post-stenotic region of model arteries were analyzed to explore their correlation with FFR. While both high and low FFR cases exhibited similarities with an inner low-age region and an outer high-age region, low FFR cases displayed asymmetry in their low-age regions due to eccentric stenosis. Moreover, gradients of age across the artery diameter were more pronounced for low FFR cases. Additionally, low FFR cases demonstrated significantly higher TKE in the post-stenotic blood jet stream compared to high FFR cases that are reflected in low-age boundary …

Regarding The Proclamation, “Private Property Is The Source Of Freedom In America, Yeomin Yoon

KAUPA Letters

No abstract provided.

Risk Analysis Of Bacteria Exposure From Students' Snacks Consumption In Elementary Schools Of Biringkanaya District, Makassar City, Nuralia, Anwar Mallongi, Syamsuar, Agus Bintara Birawida, Apik Indarty Moedjione, Healthy Hidayanty

Journal of Dentomaxillofacial Science

Indonesia is one of the countries that is rich in food. Street food in Indonesia is one of the attractions recognized worldwide. The habit of consuming snacks among schoolchildren is difficult to change, so strict inspection and supervision of snacks sold around elementary schools are needed. This study aimed to determine the risk of exposure to bacteria from the consumption of student snacks in elementary schools in Biringkanaya District, Makassar City. This type of research uses an observational method with a Quantitative Microbial Risk Assessment (QMRA) approach, which aims to calculate the health risk due to exposure to micro bacteria …

An Ex Vivo Study Of Reuterin's Root Canal Irrigation Effect On Endodontic Biofilm Gene Expression, Armelia S. Widyarman, Muhammad I. Rizal, Thalianabilla Khalisha, Denisa N. Amanda, Deandra Cn. Wiriawan

Journal of Dentomaxillofacial Science

Objective: To assess the efficacy of irrigation solution containing reuterin in inhibiting gene expression of E. faecalis, F. nucleatum, and P. gingivalis during biofilm formation in ex vivo root canal model. Material and Methods: Reuterin was isolated from an Indonesian strain of L. reuteri. Seventy-two premolar root samples were subjected to inoculation with E. faecalis, F. nucleation, and P. gingivalis for 24 hours. Subsequently, the specimens underwent irrigation with a saline solution as negative control, 25% sodium hypochlorite (NaOCl) as positive control, 25 pg/ml of reuterin derived from a L. reuteri strain indigenous to Indonesia, and …

Developing Educational Tools For Sustainable Stormwater Management, Lauren Houskeeper

All Graduate Reports and Creative Projects, Fall 2023 to Present

Rapid population growth and development in Western states are exerting strain on the region’s limited water resources. Urbanization exacerbates this issue by increasing impervious surfaces, limiting infiltration of precipitation during storm events and snowmelt, which results in changes to hydrologic conditions with higher runoff volumes and higher peak flows. Stormwater transports pollutants as it flows across impervious surfaces, discharging high volumes of runoff and elevated loads of urban contaminants into receiving waters. The amount of pollution entering waterways continually increases as urban areas expand. Utah is currently experiencing a rapid transition from undeveloped to developed landscapes, necessitating the implementation of …

A Study Of Aquatic Sedimentation In Chattanooga, Tennessee, Using Gis And Remote Sensing Technology Integrated With Hydrodynamic And Watershed Models, Connor Firat

Masters Theses and Doctoral Dissertations

Suspended sediments can have benefits and drawbacks on aquatic ecosystems depending on the concentrations. The need to quantitatively monitor water bodies and watersheds is key to reduce excessive suspended concentrations. This study aims to integrate remote sensing technology with numerical watershed and hydrodynamic models and apply them on the Tennessee River and the surrounding tributaries to better assess the fate and transport of suspended sediment concentrations (SSC) there. The obtained results indicate that using Google Earth Engine (GEE), Landsat satellite can be used to estimate historical turbidity in the Tennessee River. Using field measurements and laboratory analyses the obtained satellite …

Topology And Geometry-Based Methods For Quantifying The Complexity Of Protein Structures, Masumi Sugiyama

Masters Theses and Doctoral Dissertations

New topological and geometrical methods in knot theory provide a way to rigorously measure the entanglement of open curves in 3-space for the first time. These methods enable the topological analysis of physical systems of filaments, such as those formed by proteins. In this dissertation we employ tools from knot theory in combination with available experimental data and molecular simulations to analyze the topological complexity of tau proteins. These proteins are involved in a class of neurodegenerative diseases, called tauopathies. The methods developed in this dissertation are general and applicable to other proteins as well as any physical systems of …

Predicting Hand Grasping Orientation For Prosthetic Hand Control Using Multimodal Sensor Data (Eeg, Emg, And Imu) With Machine Learning Approach, Nafiseh Ghaffar Nia

Masters Theses and Doctoral Dissertations

Enhancing the control of prosthetic hands is a crucial challenge that directly impacts the daily functionality of individuals with limb loss. Our research delves into advanced machine learning (ML) methodologies to accurately predict hand-grasping orientations, thereby improving the precision of prosthetic control. We present a comprehensive framework that amalgamates inputs from multiple sensors. This includes electroencephalography (EEG) to discern user intentions, electromyography (EMG) to evaluate muscle activity, and inertial measurement units (IMU) to track features of hand movement. By harmoniously integrating these varied data streams, our ML model strives to provide predictions of hand orientation that are more accurate and …

High Fat Diet & Social Isolation: Interactive Effects On Pain, Cognition, & Neuroinflammation, Ian M. Campuzano

Research Psychology Theses

Prior research has established a role for both social isolation and exposure to high fat Western diets in altering a range of behaviors from reduced memory performance to increased depression-like behaviors. The present study scrutinizes the interplay among these variables during the peri-adolescent developmental phase, utilizing Long-Evans rats as the experimental model. Our overarching hypothesis is that rats exposed to either social isolation, a high-fat diet, or both will result in heightened pain sensitivity, diminished cognitive flexibility, and increased neuroinflammatory responses within brain regions implicated in sociability, cognition, memory, and pain processing. Behavioral flexibility will be assessed using a maze-based …

Factors Impacting Treatment Decisions In Caregivers Of Autistic Children, Tiffany Harris

Dissertations

Caregivers of autistic children are not only tasked with deciding upon various treatment options for their children’s symptoms but must also navigate the initial crisis that is experienced after receiving a diagnosis of ASD for their children (Blancher, 1984; Ellis, 1989). Leventhal’s Common Sense Model of Self-Regulation (the CSM; Leventhal et al., 1980) is a theoretical framework developed to understand the self-regulatory processes involved in adapting to and managing health threats and posits that cognitive and emotional illness perceptions influence illness outcomes through coping behaviors. This is the first study to examine all major components of the CSM (i.e., cognitive …

Examining The Association Between Parental Attachment, Fictive Kin Bonds, Psychological Needs Satisfaction, Emotional Distress, And Subjective Well-Being Among Black Americans, Roxanne Watts

Dissertations

Secure attachment with parents predicts less emotional distress and greater subjective well-being across the lifespan. One mechanism by which parental attachment supports mental health outcomes is through psychological needs satisfaction (PNS). In addition to the attachment to parents, relationships with fictive kin caregivers are prevalent and important among Black families. The present study investigated the association between parental attachment, fictive kin bonds, psychological needs satisfaction and mental health outcomes (i.e., emotional distress and subjective well-being) in a sample of Black American adults. Two hundred twenty-three (N = 223) participants completed measures of parental attachment, fictive-kin bonds, psychological needs satisfaction …