Digital Commons Network^™

Overnight Rice Fermentation: An Analysis Of The Physicochemical Changes In White, Brown, And Enriched Rice Varieties, Kavin Sivakumar

Master's Theses

Fermentation refers to the activity of microorganisms breaking down substrates, resulting in changes to a food product. One fermented food practice from the Indian subcontinent is overnight fermented rice. The traditional preparation consists of covering cooled cooked rice in water and leaving it at room temperature to ferment overnight. The objective of this project was to investigate overnight rice fermentation through quantifying its physicochemical changes. The study utilized three types of rice: white, enriched white, and brown. Mixtures of cooked rice and water at a 1 gram: 2 milliliter ratio were fermented at 29 degrees Celsius and 60% humidity for …

Research-Based Early Literacy Practices For Lower Elementary Educators, Erica Joy Austin

Masters Projects

Low literacy rates are a significant issue in the United States. Low literacy skills correlate with many notable societal issues including poverty, higher chances of unemployment and incarceration, and even poor health. There are many significant contributors to the issue of low literacy including but not limited to poverty, teacher attrition, chronic student absenteeism and tardiness, lack of literacy practices taking place in homes, and lack of research-based literacy practices taking place in elementary classrooms. Although all of these issues significantly affect the literacy rates in the United States, among all of these issues, teachers have the most control over …

Mitochondrial Permeability Transition Dictates Mitochondrial Maturation Upon Switch In Cellular Identity Of Hematopoietic Precursors, Sandeep P Dumbali, Paulina D Horton, Travis I Moore, Pamela L Wenzel

Faculty, Staff and Student Publications

The mitochondrial permeability transition pore (mPTP) is a supramolecular channel that regulates exchange of solutes across cristae membranes, with executive roles in mitochondrial function and cell death. The contribution of the mPTP to normal physiology remains debated, although evidence implicates the mPTP in mitochondrial inner membrane remodeling in differentiating progenitor cells. Here, we demonstrate that strict control over mPTP conductance shapes metabolic machinery as cells transit toward hematopoietic identity. Cells undergoing the endothelial-to-hematopoietic transition (EHT) tightly control chief regulatory elements of the mPTP. During EHT, maturing arterial endothelium restricts mPTP activity just prior to hematopoietic commitment. After transition in cellular …

Exploring How Caregiver Multiracial Socialization Is Related To Multiracial Identity Pride, Self-Esteem, And Racial Political Identity In Emerging Adulthood, Jennie Jasperson

Theses and Dissertations

The current study examined how caregiver multiracial socialization processes play a role in multiracial emerging adults' multiracial identity pride self-esteem, and racial political identity. The constructs of caregiver multiracial socialization, multiracial identity pride in emerging adulthood, sense of self-esteem in emerging adulthood, and racial political identity in emerging adulthood were examined among 387 multiracial emerging adults between the ages of 18-29 from across the United States. To examine these relationships, multiple regressions were run to analyze data. The results of this study underscore the significance of understanding the role that socialization processes within multiracial families play in ethnic racial identity …

Emergent Themes In Identity Development For Transracial Adoptees: An Interpretative Phenomenological Analysis, Andrew G. Cecil

Doctoral Dissertations

The purpose of the study was to examine the identity development processes of transracial adoptees in college, using Interpretative Phenomenological Analysis (IPA) to explore the lived experiences of individuals from this community through in-depth interviewing. This study identified nine superordinate themes and eighteen sub-themes providing knowledge on the dynamic processes of multidimensional identity development of transracial adoptees in college. Despite variability and differences across participant experiences, these themes function as major paradigms illustrating participants’ understanding of their respective identity development and lived experiences. The results of this study may serve as a basis for further exploration of transracial adoptees’ identity …

3d Chromatin Architecture, Brd4, And Mediator Have Distinct Roles In Regulating Genome-Wide Transcriptional Bursting And Gene Network, Pawel Trzaskoma, Seolkyoung Jung, Aleksandra Pękowska, Christopher H Bohrer, Xiang Wang, Faiza Naz, Stefania Dell'orso, Wendy D Dubois, Ana Olivera, Supriya V Vartak, Yongbing Zhao, Subhashree Nayak, Andrew Overmiller, Maria I Morasso, Vittorio Sartorelli, Daniel R Larson, Carson C Chow, Rafael Casellas, John J O'Shea

Faculty, Staff and Student Publications

Discontinuous transcription is evolutionarily conserved and a fundamental feature of gene regulation; yet, the exact mechanisms underlying transcriptional bursting are unresolved. Analyses of bursting transcriptome-wide have focused on the role of cis-regulatory elements, but other factors that regulate this process remain elusive. We applied mathematical modeling to single-cell RNA sequencing data to infer bursting dynamics transcriptome-wide under multiple conditions to identify possible molecular mechanisms. We found that Mediator complex subunit 26 (MED26) primarily regulates frequency, MYC regulates burst size, while cohesin and Bromodomain-containing protein 4 (BRD4) can modulate both. Despite comparable effects on RNA levels among these perturbations, acute depletion …

Family Functionality And Social-Emotional Learning Skills For Middle School Students: A Predictive Correlational Study, Elisabeth Anne Chapman

Doctoral Dissertations and Projects

The purpose of this non-experimental, quantitative, predictive correlational study was to determine if there was a predictive relationship between family functionality, based on the linear combination of expressiveness, cohesion, conflict, and social-emotional learning skills for middle school students. The findings of this study provided data to better understand the extent to which home life impacts the ability of middle school students to apply social-emotional skills at school and can provide educators with data to use as a baseline in understanding and evaluating the current levels of a child’s social-emotional learning skills. The research observed 80 middle school students, Grades 6-8, …

Rheumatoid Arthritis Disease Activity Is Associated With Frailty In Veterans, Courtney N. Loecker

Theses & Dissertations

Frailty, a physiologic vulnerability to everyday stressors, affects approximately 30% of the 1.3 million Americans with rheumatoid arthritis (RA) and is associated with increased risk for adverse health outcomes. Active RA manifests as inflammation, pain, and joint damage leading to functional impairments and morbidity which cumulatively influences frailty. However, the relationship between RA disease activity and frailty is incompletely understood.

This study evaluated the relationship of RA disease activity and frailty at baseline and over up to five years in Veterans. A cohort study was performed using data from the Veterans Affairs Rheumatoid Arthritis registry. RA disease activity was measured …

Pharmacokinetics And Tissue Distribution Of A Lipid-Based Extended-Release Nano-Formulation Of Mo-Oh-Nap Tropolone To Enhance Pulmonary Delivery For The Treatment Of Pulmonary Metastatic Osteosarcoma, Wafaa N. Aldhafiri

Theses & Dissertations

Osteosarcoma is the most common primary bone tumor in children and adolescents. Metastasis is prevalent in 25% of patients, at diagnosis, with the lungs being the most common metastatic site. Pulmonary metastatic osteosarcoma is a significant therapeutic challenge with a 5-year survival rate of ≤30%. Patients with pulmonary metastatic disease at diagnosis receive the same standard of care as patients with local disease, which hasn’t changed over the past 30 years. The treatment and prevention of pulmonary metastatic osteosarcoma represents a significant critical and unmet need.

MO-OH-Nap tropolone is a novel small molecule with cytotoxic activity across multiple human osteosarcoma …

Understanding The Role Of Mastl In Colon Homeostasis And Colitis-Associated Cancer Development, Kristina Pravoverov

Theses & Dissertations

Colitis-associated cancer (CAC) is the most devastating complication of longstanding inflammatory bowel disease (IBD). Understanding the mechanisms underlying the switch from damage-repair to dysplasia in IBD is critical for identifying novel therapies to facilitate mucosal healing while mitigating CAC risk. Microtubule-associated serine-threonine kinase-like (MASTL), though crucial in cell growth and repair, is upregulated in sporadic colorectal cancer and CAC. This study sought to delineate the role of MASTL in colitis and CAC development.

First, we examined whether MASTL is associated with colitis and recovery. Review of publicly available data found significant elevation of MASTL and interleukin (IL)-22 in biopsies from …

The Development Of Tissue-Specific Glucocorticoid Prodrug Nanomedicines For The Treatment Of Inflammatory/Autoimmune Diseases, Haochen Jiang

Theses & Dissertations

Addressing chronic inflammatory diseases such as focal segmental glomerulosclerosis (FSGS) and psoriasis requires treatments that not only provide effective relief but also minimize adverse effects. Central to our approach is the targeted delivery of potent anti-inflammatory and immune suppressive glucocorticoids (GC) directly to the sites of tissue pathology. This strategy enhances therapeutic efficacy and significantly reduces the side effects typically associated with GC therapy.

In the case of FSGS, a rare nephritis characterized by scarring and podocyte injury, we have developed a polyethylene glycol (PEG)-based dexamethasone (Dex) prodrug (PEG-Dex). This formulation focuses the drug's activity on the kidneys, limiting off-target …

Novel Mechanisms For The Neural Control Of Breathing In Acute Lung Injury, Kajal Kamra

Theses & Dissertations

Acute lung injury (ALI) triggers inflammation that disrupts the normal alveolar-capillary endothelial barrier, impairing gas exchange and causing hypoxemia, which reflexively increases respiration. An elevated respiratory rate (fR) usually develops within hours to days following ALI. This acute respiratory failure disorder affects approximately 200,000 new cases annually in the US and accounts for 10% of ICU admissions, with high morbidity and mortality. The neural mechanisms underlying respiratory dysfunction post-ALI are not fully understood. My doctoral dissertation aimed to investigate the mediators of abnormal ventilation during ALI. Systemic hypoxia stimulates the carotid body (CB) chemoreflex, a crucial protective reflex that maintains …

Molecular Characterization Of Viral Escape From Art And Crispr Hiv-1 Excision In Infected Humanized Mice, Chen Zhang

Theses & Dissertations

Not available.

A Third Generation Long-Acting Dimeric Dolutegravir Prodrug Nanoformulation, Bhoomika Suresh Gowda

Theses & Dissertations

Dolutegravir (DTG) is an integrase strand transfer inhibitor (INSTI) widely used as a part of first-line combination antiretroviral therapy. DTG has a high barrier to resistance However, one therapeutic challenge centers on its life-long daily use. Sub-optimal adherence to daily oral pills underscore the need for long-acting formulations. To these ends, our research activities have focused on the development and translation of ultra-long-acting ART. Monomeric ester prodrugs provided initial success in extending the apparent half-life of DTG and other INSTIs. One is the DTG prodrug formulation called NM2DTG. NM2DTG’s significantly extended the parent drug’s apparent half-life [Nature Communications, 3226 (2022)]. …

Dying Right: Supporting Anti-Cancer Therapy Through Immunogenic Cell Death, Elizabeth Schmitz

Theses & Dissertations

The primary goal of most anti-cancer therapy is to eradicate neoplastic cells. Simultaneously, neoplastic cell death is the first step of the cancer-immunity cycle, which starts with the release of antigens from cancer cells and ends with T-cell directed killing of cancer cells, in addition to long-standing memory to tumor antigens encountered. Immunogenic damage-associated-molecular patterns (DAMPs) are endogenous cell molecules repurposed in times of insurmountable cell stress (e.g., CALR, ATP, HMGB1) to facilitate communication between dying cells and adaptive immune cells, acting as adjuvants for the cancer-immunity cycle, in a cellular fate termed immunogenic cell death (ICD). It …

Delivery Of Small Molecule Using Polymeric Nanoparticles For The Treatment Of Alcoholic Liver Disease And Its Pharmacokinetics And Biodistribution In Mice, Jingyi Ma

Theses & Dissertations

Alcoholic liver disease is a challenging chronic disease worldwide. Alcohol abuse and subsequent inflammation, oxidative stress, and disordered lipid metabolism result in liver damage. This dissertation aims to develop a reliable therapeutic strategy for alcoholic liver disease treatment. We have employed polymeric nanoparticles as the delivery system to the liver and utilized PDE4B inhibitor as a novel approach for alcoholic liver disease treatment.

Chapter 1 is an overview of alcoholic liver disease, including the background, pathology, risk factors, and current treatment. In addition, the application of PDE4 inhibitors in alcoholic liver disease was discussed, suggesting a promising avenue for future …

Chemotherapy-Induced Modulation Of Tumor Antigen Presentation, Alaina C. Larson

Theses & Dissertations

Pancreatic cancer is a lethal malignancy, with a five-year overall survival rate of just 13%. Current treatment approaches thus require modification and attention has shifted towards improving immunotherapy efficacy. Select chemotherapy drugs possess inherent immunomodulatory activity and could be used to initiate immunotherapeutic success, thereby restoring anti-tumor immunity in pancreatic cancer. In this work, I evaluated the impact of the chemotherapy drug gemcitabine on tumor antigen presentation by human leukocyte antigen class I (HLA-I). Gemcitabine regulated HLA-I expression on multiple levels, increasing pancreatic cancer cells’ HLA-I mRNA transcripts, total protein and surface expression, as well as surface stability. Temperature-dependent assay …

Body Length Determines Flow Refuging For Rainbow Trout (Oncorhynchus Mykiss) Behind Wing Dams, Terry R. Dial, Laura A. Collins, James C. Liao, Bret W. Tobalske

Environment and Society Faculty Publications

Complex hydrodynamics abound in natural streams, yet the selective pressures these impose upon different size classes of fish are not well understood. Attached vortices are produced by relatively large objects that block freestream flow, which fish routinely utilize for flow refuging. To test how flow refuging and the potential harvesting of energy (as seen in Kármán gaiting) varies across size classes in rainbow trout (Oncorhynchus mykiss; fingerling, 8 cm; parr, 14 cm; adult, 21 cm; n = 4 per size class), we used a water flume (4,100 L; freestream flow at 65 cm s^-1) and created …

Modulation Of The Internal Wave Regime Over A Tropical Seamount Ecosystem By Basin-Scale Oceanographic Processes, Ted Robinson, Philip Hosegood, Adam Bolton

School of Biological and Marine Sciences

Shallow seamounts are becoming increasingly recognised as key habitats for conservation due to their role asbiological refuges, particularly throughout oligotrophic oceans. Traditionally, Taylor caps have been invoked asthe mechanism driving biomass aggregation over seamounts but emerging evidence based on higher resolutionmeasurements highlights the importance of internal waves (IW) to the local ecosystem. These waves can flush thebenthic habitat with cool water from depth and impact on nutrient supply over short time scales through turbulentmixing that may also influence fish behaviour. They are dependent on the regional stratification, however,and thus influenced by planetary-scale variability in oceanographic conditions. We present here detailed …

Re: Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites Field Sampling Plan (Fsp) Bres No. 121 – Travona Dump., Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Role Of Pycr2 In Colorectal Cancer, Raju Lama Tamang

Theses & Dissertations

Despite significant progress in clinical management, colorectal cancer (CRC) remains the 3rd most common cause of cancer-related deaths. A positive association of PYCR2 (Pyrroline-5-carboxylate reductase-2), a terminal enzyme of proline metabolism, with CRC aggressiveness was recently reported including by us. However, how PYCR2 promotes colon carcinogenesis remains ill understood. In our studies, comprehensive in-silico and biochemical evaluations were performed along with genetic manipulations and in-vivo tumor growth assays to gain mechanistic understanding. Results showed that PYCR2 expression was significantly upregulated in CRC and associated with poor patient survival specifically among PYCR isoforms (PYCR-1, -2 and -3). Genetic inhibition of PYCR2 …

Asporin/Crabp2 Axis Drives "Cell-State" Transition To Enable Neuroendocrine-Like Phenotype In Prostate Cancer, Shobhit P. Lall

Theses & Dissertations

Prostate cancer (PCa) stands as the top frequently diagnosed malignancy and the second-most leading cause of death in males in the United States in 2024. Androgen-targeted therapies are effective for early-stage PCa. However, it recurs with castration resistant PCa, leading to survival for less than 2 years. Further advancement of PCa causes it to metastasize and develop “de novo” and treatment related neuroendocrine PCa cancer (NEPC), a lethal form of PCa with a poor survival of 7 months. Hence, to understand the biology beyond PCa metastasis, we developed a composite mouse model with loss of Pten and p53 function and …

Determining If Cx45 Acts As An Arrythmogenic Substrate In The Diseased Myocardium, Stephen R. Sobota

Theses & Dissertations

Proper contraction of the heart relies on gap junction channels (GJCs) composed of Cx40 and Cx43 in the atrial and ventricular tissues which mediate electrical conductance in the working myocardium. Also expressed is Cx45, which is limited to the pacemaker system. Studies in diseased human hypertrophic cardiac tissue have shown expression of Cx45 and decrease of Cx43 in the myocardium. Cx43 and Cx45 in vitro form heterometric GJCs, where the channel characteristics (metabolite transfer, electrical conductance, etc.) resemble those of Cx45. There is little-to-no data showing whether GJCs composed of Cx43 and Cx45 in cardiac hypertrophy result in altered conductance. …

Targeting Ksr1 To Inhibit Stemness And Therapy Resistance, Heidi M. Vieira

Theses & Dissertations

Tumor heterogeneity is an important contributor to therapy resistance and relapse. Within the tumor, there are subpopulations of tumorigenic, self-renewing cells with enhanced clonogenicity and chemoresistance, termed tumor-initiating cells (TICs). Despite their importance in treatment response, there are no effective therapies to target TICs in solid tumors.

This study identifies a novel role for Kinase Suppressor of Ras 1 (KSR1) in regulating TICs and therapy resistance in Ras-driven cancers. KSR1 is a molecular scaffold in the Raf/MEK/ERK kinase cascade downstream of Ras. KSR1 is necessary for Ras-mediated transformation, but dispensable for normal cell growth; further, ksr1^-/- mice are phenotypically …

Re: Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites Field Sampling Plan (Fsp) Bres No. 49 – Old Glory., Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Bmfou Berkeley Pit Waterfowl Protection Program - Request For Change, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Re: Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites Field Sampling Plan (Fsp) Bres No. 125 – Child Harold-2 Dump., Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.

Qtl Mapping For Fatty Acid Composition In Olive Oil Using A High-Density Genetic Map Based On Snp Markers, Ali̇ Can Kaya, Meryem İpek, Ahmet İpek, Mehmet Ali̇ Gündoğdu, Nesri̇n Aktepe Tangu, Sevi̇n Teoman Duran, Murat Şeker, Mustafa Akbulut

Turkish Journal of Agriculture and Forestry

Olive (Olea europaea L.) is an evergreen tree species that grows naturally in regions with Mediterranean climates. Its oil and fruits are commercially valuable. Olive oil contains high levels of omega-9 (oleic acid). Because the high percentage of oleic acid makes olive oil deterioration-resistant, the development of olive varieties containing high oleic acid is one of the major goals of olive breeding programs. Therefore, this study aimed to determine quantitative trait loci (QTL) affecting fatty acid composition of olive oil. Thus, early selection of olive genotypes with a high oleic acid content can be possible. For the determination of QTLs …

Legal Regime Of The Application Process For Gmo Products In Türkiye, Ünal Yildiz, Özgür Temi̇z, Kami̇l Hali̇loğlu, Nesri̇n Yildiz

Turkish Journal of Agriculture and Forestry

In Turkiye, in accordance with EU harmonization laws, Biosafety Act No. 5977 entered into force on 18.09.2010. The basic legislation regarding the application process is composed of the Biosafety Act, Regulations on Genetically Modified Organisms and Their Products, and Regulations on Working Procedures and Principles of Biosafety Board and Committees. Biosafety legislation foresees a special application procedure for GMO food, food products, feed, and other products. The application regulated in the Biosafety Law is a special administrative procedure in nature. The primary objective of this study is to explain the legal regime of this application procedure. While explaining this issue, …