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Bp1003 Decreases Stat3 Expression And Its Pro-Tumorigenic Functions In Solid Tumors And The Tumor Microenvironment, Maria Gagliardi, Rhonda Kean, Bingbing Dai, Jithesh Augustine, Michael Roberts, Jason Fleming, D. Craig Hooper, Ana Ashizawa

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Overexpression and aberrant activation of signal transducer and activator of transcription 3 (STAT3) contribute to tumorigenesis, drug resistance, and tumor-immune evasion, making it a potential cancer therapeutic target. BP1003 is a neutral liposome incorporated with a nuclease-resistant P-ethoxy antisense oligodeoxynucleotide (ASO) targeting the STAT3 mRNA. Its unique design enhances BP1003 stability, cellular uptake, and target affinity. BP1003 efficiently reduces STAT3 expression and enhances the sensitivity of breast cancer cells (HER2⁺, triple negative) and ovarian cancer cells (late stage, invasive ovarian cancer) to paclitaxel and 5-fluorouracil (5-FU) in both 2D and 3D cell cultures. Similarly, ex vivo and in …

Hydroxyurea In The Sickle Cell Disease Modern Era, Chazmyn Riley, Walter K. Kraft, Robin Miller

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

INTRODUCTION: Sickle cell disease is an inherited disorder characterized by hemoglobin S polymerization leading to vaso-occlusion and hemolytic anemia. These result in a variety of pathological events, causing both acute and chronic complications. Millions around the world are affected by sickle cell disease with predominance in sub-Saharan Africa. Hydroxyurea was the first drug approved for use in sickle cell disease to reduce the occurrence of painful crises and blood transfusions in patients with frequent, moderate to severe painful crises.

AREAS COVERED: With the development of new therapeutics, the role of hydroxyurea is evolving. This narrative review aims to provide clinical …

Evaluating The Communication And Accountability Of Nurses In The Clinic Setting, Emily Baarz

Walden Dissertations and Doctoral Studies

Research has demonstrated the importance of effective communication and its relationship to positive patient, provider, and organizational outcomes. In the local organization, nursing staff verbalized concerns regarding lack of professional communication that may contribute to a hostile work environment and affect the quality of patient care delivered. To address this concern, the organization implemented a staff education program focused on effective communication practices and level of accountability. The purpose of this quality improvement project was to determine if the educational intervention focused on effective communication practices and level of accountability increased the participants’ perceptions of communication and accountability among nursing …

Synthesis And Characterization Of Nial-Hydride Heterometallics: Perturbing Electron Density Within Al–H–Ni Subunits, Aleida G. Gonzalez, Fernando Gonzalez, Edgar De Leon, Kaitlyn M. Birkhoff, Sam Yruegas, Haoyuan Chen, Manar Shoshani

School of Integrative Biological & Chemical Sciences Faculty Publications

Heterometallic hydride complexes are of growing interest due to their potential to contribute to highly active insertion-based catalysis; however, methods to modulate electron density within this class of molecules are underexplored. Addition of ancillary ligands to heterotrimetallic NiAl2H2 species (1) results in the formation of heterobimetallic NiAl-hydride complexes with varying phosphine donors (2-(L)2). Incorporation of sigma donating ancillary ligands of increasing strength led to contractions of the Ni–Al distances correlated to a strengthening of a back donation interaction to the Al–H sigma antibonding orbital, most prominently present in 2-(PMe3)2. Demethylation of the aryl ether from 2-(PMe3)2 provides access to a …

Identifying The Interactors Of Otud6b-2 That Are Important For Its Function On C-Myc, Farah Albatsh

Master's Theses

The complexity of molecular signaling pathways, particularly in cancer research, has made the proto-oncogene c-Myc a key focus due to its role as a master regulator of cellular processes such as growth, division, and apoptosis. Dysregulation of c-Myc is linked to various cancers, making it a highly sought therapeutic target despite challenges in directly inhibiting its function. Recent efforts have shifted towards indirect targeting methods, including BET inhibitors, cyclin-dependent kinase inhibitors, and siRNA formulations. A novel approach using PROTACs and the investigation of c-Myc-specific deubiquitinases (DUBs) has shown potential, particularly the emerging player OTUD6B-2. This study aims to identify and …

Structural Basis For Substrate Binding And Selection By Human Mitochondrial Rna Polymerase, Karl Herbine, Ashok Nayak, Dmitry Temiakov

Department of Biochemistry and Molecular Biology Faculty Papers

The mechanism by which RNAP selects cognate substrates and discriminates between deoxy and ribonucleotides is of fundamental importance to the fidelity of transcription. Here, we present cryo-EM structures of human mitochondrial transcription elongation complexes that reveal substrate ATP bound in Entry and Insertion Sites. In the Entry Site, the substrate binds along the O helix of the fingers domain of mtRNAP but does not interact with the templating DNA base. Interactions between RNAP and the triphosphate moiety of the NTP in the Entry Site ensure discrimination against nucleosides and their diphosphate and monophosphate derivatives but not against non-cognate rNTPs and …

Data From: Sheared Turbulent Flows And Wake Dynamics Of An Idled Floating Tidal Turbine, Lilian Lieber, Alex Nimmo Smith

Faculty of Science and Engineering Datasets

Input data (minimally processed ADCP Matlab files) used to generate this study's results. The data is divided into two folders: 1) Broad-scale Transects and 2) Fine-scale Transects and 3), a README file is provided.

Informatics-Based Discovery Of New Natural Products, Their Biosynthesis, And Their Biological Roles, Ethan Older

Theses and Dissertations

In this work, genome mining and biosynthetic knowledge are applied for the discovery of new natural products, their biosynthetic pathways, and their promising biological activities and roles through the development of two unique new informatics-based approaches.

In chapter one, an overview of microbial natural product biological functions and biosynthesis is provided. The use of bioinformatics leveraging microbial natural product biosynthetic knowledge is also introduced. This chapter highlights the importance and need for developing innovative approaches to accessing microbial natural products, a vastly untapped source of new molecules with promising biological activities.

In chapter two, a correlational network linking the gene-encoded …

Draft Genomes Of Neisseria Perflava Umb0578, Proteus Mirabilis Umb8339, And Enterococcus Faecalis Umb7967 Isolated From Urine Samples, Alex Kula, Ali Khan, Megan Martinez, Jevan Terry, Helen Appleberry, Alan J. Wolfe, Catherine Putonti

Biology: Faculty Publications and Other Works

The urinary tract of females harbors a variety of microorganisms, both for those with and without symptoms. Here, we present the draft genome sequences of three isolates from urine samples—Neisseria perflava UMB0578, Proteus mirabilis UMB8339, and Enterococcus faecalis UMB7967.

Draft Genomes Of One Staphylococcus Haemolyticus And Five Staphylococcus Lugdunensis Strains Isolated From Catheterized Urine Samples Of Females, Helen Appleberry, Haaris Anjum, Taleah Cage, Kayla Jarm, Haashir Khan, Lizzie Proctor, Junelle Saroca, Alan J. Wolfe, Catherine Putonti, Alex Kula

Biology: Faculty Publications and Other Works

Although Staphylococcus haemolyticus and Staphylococcus lugdunensis are members of the normal human flora, they also can cause infection. Here, we present the draft genomes of five strains of S. lugdunensis and one strain of S. haemolyticus isolated from transurethral catheterized urine samples from different females experiencing lower urinary tract symptoms.

Investigations Of Cell Tower Antennas Parameters On Reduction Of Radio Frequency Radiation Levels From Radio Base Stations, Florence U. Rashidi

Tanzania Journal of Engineering and Technology (TJET)

The widespread deployment of mobile cellular base stations in populated areas has raised public health concerns due to increased exposure to radio frequency (RF) radiation emissions. Exposure to high levels of RF radiation can have potential thermal and non-thermal biological effects. Optimizing the configuration of cell tower antenna parameters is crucial for mitigating these radiation levels. This study therefore aims at systematically investigating the influence of different cell tower antenna parameters on reducing the RF radiation levels from mobile base stations. Field measurements were conducted at two cell sites shared by multiple mobile operators. Electric field strengths were measured at …

Synergetic And Orthogonal Switching In Dual Photochromic Metal-Organic Frameworks, Gina Rene Wilson

Theses and Dissertations

Cooperative behavior and orthogonal responses of two classes of coordinatively integrated photochromic molecules towards distinct external stimuli were demonstrated on the first example of a photo-thermo-responsive hierarchical platform. Synergetic and orthogonal responses to temperature and excitation wavelength are achieved by confining the stimuli-responsive moieties within a metal-organic framework (MOF), leading to the preparation of a novel photo-thermo-responsive spiropyran-diarylethene based material. Synergistic behavior of two photoswitches enables the study of stimuli-responsive resonance energy transfer as well as control of the photoinduced charge transfer processes, milestones required to advance optoelectronics development. Spectroscopic studies in combination with theoretical modeling revealed a nonlinear effect …

Development Of A Urothelial Cell Line Expressing A Caspase-1 Biosensor To Measure Cellular Inflammatory Responses To Potential Uropathogens, Nikita Riya Kesav

Master's Theses

Urinary tract infections are one of the most common bacterial infections, historically implicating Escherichia coli as the causative pathogen. However, it is now known that there are a variety of bacteria that exist within the urinary tract, acting as either beneficial uroprotectives or opportunistic uropathogens. Furthermore, there are significant gaps in our understanding as to how these bacteria interact with the urothelium and what role each plays in maintaining or disrupting homeostasis. Research has shown that Toll-like receptor 4 (TLR4) signaling and Nod-like Receptor Protein 3 play a role in inflammasome activation during infection, triggering caspase-1 activation and inducing release …

Evolutionary Patterns Of Anolis Lizard Skulls: An Investigation In Geometric Morphometrics And An Educational Exploration Of Structure And Function, Katherine Beyer Starr

Master's Theses

This study explores the evolutionary patterns of Anolis lizard skulls and integrates findings into a curriculum focused on elucidating the structure and function of a lizard's head. The first objective was to develop an understanding of how the evolutionary patterns, of an extraordinary group of lizards (Anolis) compares to their relatives. Most of the prior research on Anolis morphology has focused on the post-cranium because of its significance towards subdivision of the arboreal habitat. But the group’s remarkable diversity in head shape has been seriously under-investigated. By performing geometric morphometric analysis of skull shape across a large sample of anoles …

Regulation Of Sterile Inflammatory Responses By Mitochondria-Associated Proteins, Meghana Venkatesan

Master's Theses

Inflammation is an important defense mechanism through which cells respond to pathogenic insults and damage/stress signals. Immune cells mediate extracellular inflammatory responses by synthesizing and releasing small proteins called cytokines, which play critical roles in modulating these responses. Specifically, key pro-inflammatory cytokines such as IL-1beta are released upon the assembly and activation of a multi-protein intracellular complex called the inflammasome, the most well-studied of which is the NLRP3 inflammasome. Several proteins have been identified as important regulators of cellular stress responses, including vacuole membrane protein 1 (VMP1). The present research shows that VMP1-depleted cells exhibit exacerbated cellular inflammation and are …

Mechanism Of Gabapentinoid Potentiation Of Opioid Effects On Cyclic Amp Signaling In Neuropathic Pain, Anibal Garza-Carbajal, Alexis Bavencoffe, Juan J Herrera, Kayla N Johnson, Edgar T Walters, Carmen W Dessauer

Faculty, Staff and Student Publications

Over half of spinal cord injury (SCI) patients develop opioid-resistant chronic neuropathic pain. Safer alternatives to opioids for treatment of neuropathic pain are gabapentinoids (e.g., pregabalin and gabapentin). Clinically, gabapentinoids appear to amplify opioid effects, increasing analgesia and overdose-related adverse outcomes, but in vitro proof of this amplification and its mechanism are lacking. We previously showed that after SCI, sensitivity to opioids is reduced by fourfold to sixfold in rat sensory neurons. Here, we demonstrate that after injury, gabapentinoids restore normal sensitivity of opioid inhibition of cyclic AMP (cAMP) generation, while reducing nociceptor hyperexcitability by inhibiting voltage-gated calcium channels (VGCCs). …

Clinical And Genomic-Based Decision Support System To Define The Optimal Timing Of Allogeneic Hematopoietic Stem-Cell Transplantation In Patients With Myelodysplastic Syndromes, Cristina Astrid Tentori, Caterina Gregorio, Marie Robin, Nico Gagelmann, Carmelo Gurnari, Somedeb Ball, Juan Carlos Caballero Berrocal, Luca Lanino, Saverio D'Amico, Marta Spreafico, Giulia Maggioni, Erica Travaglino, Elisabetta Sauta, Manja Meggendorfer, Lin-Pierre Zhao, Alessia Campagna, Victor Savevski, Armando Santoro, Najla Al Ali, David Sallman, Francesc Sole, Guillermo Garcia-Manero, Ulrich Germing, Nicolaus Kroger, Shahram Kordasti, Valeria Santini, Guillermo Sanz, Wolfgang Kern, Uwe Platzbecker, Maria Diez-Campelo, Jaroslaw P Maciejewski, Lionel Ades, Pierre Fenaux, Torsten Haferlach, Amer M Zeidan, Gastone Castellani, Rami Komrokji, Francesca Ieva, Matteo Giovanni Della Porta, Genomed4all And Synthema Consortiums

Faculty, Staff and Student Publications

Purpose: Allogeneic hematopoietic stem-cell transplantation (HSCT) is the only potentially curative treatment for patients with myelodysplastic syndromes (MDS). Several issues must be considered when evaluating the benefits and risks of HSCT for patients with MDS, with the timing of transplantation being a crucial question. Here, we aimed to develop and validate a decision support system to define the optimal timing of HSCT for patients with MDS on the basis of clinical and genomic information as provided by the Molecular International Prognostic Scoring System (IPSS-M).

Patients and methods: We studied a retrospective population of 7,118 patients, stratified into training and validation …

Impacts Of Interleukin-10 Promoter Genotypes On Prostate Cancer, Yu-Ting Chin, Chung-Lin Tsai, Hung-Huan Ma, Da-Chuan Cheng, Chia-Wen Tsai, Yun-Chi Wang, Hou-Yu Shih, Shu-Yu Chang, Jian Gu, Wen-Shin Chang, Da-Tian Bau

Faculty, Staff and Student Publications

Prostate cancer (PCa) is a multifactorial disease influenced by genetic, environmental, and immunological factors. Genetic polymorphisms in the interleukin-10 (IL-10) gene have been implicated in PCa susceptibility, development, and progression. This study aims to assess the contributions of three IL-10 promoter single nucleotide polymorphisms (SNPs), A-1082G (rs1800896), T-819C (rs3021097), and A-592C (rs1800872), to the risk of PCa in Taiwan. The three IL-10 genotypes were determined using PCR-RFLP methodology and were evaluated for their contributions to PCa risk among 218 PCa patients and 436 non-PCa controls. None of the three IL-10 SNPs were significantly associated with the risks of …

The Sufficiency Of Disclosure Of Ai Inventions, Mateo Aboy, Aparajita Lath, Timo Minssen, Kathleen Liddell

Articles

• The complex and data-driven nature of artificial intelligence (AI) raises questions for the sufficient disclosure of patent applications in this field. What are the European patent disclosure requirements for AI inventions?

• One challenge is that, prior to training, AI systems can be considered generic models. But after training, they transform into specialized AI systems to solve a particular problem. This transformation requires training data, making it an integral part of the AI system’s definition. But to what extent is the disclosure of the training data or training process necessary for patent disclosure?

• The Boards of Appeal of the European Patent …

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Thermal Regimes Of Beaver Impacted Stream Reaches In The Tualatin River Basin, Miles Mayer

Dissertations and Theses

Climate change and urbanization are expected to cause major changes to hydrologic regimes over the Pacific Northwest Region. The Tualatin River Basin, an area of the Pacific Northwest just west of Portland, OR has been subjected to considerable water-quality issues, including stream temperature. As ecosystem engineers, beavers have the potential to mitigate stream degradation and, consequently, there is increasing interest in beaver-related restoration. However, different conclusions have been found on beaver dam impacts to stream thermal regimes. These differences have been attributed to geographical differences in study location and variability in methods and investigated spatiotemporal scales. We investigated summer thermal …

Defining Relevant Markets In Digital Ecosystems, Daniel A. Crane

Articles

Traditional market definition focuses on the substitutability of two products or geographies. In digital ecosystems, competition often occurs in important ways that elide definition in conventional substitutability terms. This Article considers three kinds of economic rivalry that do not fit the conventional market definition mold: (1) Single-side competition: In two sided markets, firms may compete on just one side of the market. For example, Google and Facebook compete for the same advertisers, but their users on the other side of the market do not necessarily think of the offerings (social media and universal search) as substitutes; (2) Ecosystem competition: Technology …

Physical Activity Modifies The Risk Of Incident Cardiac Conduction Disorders Upon Inflammation: A Population-Based Cohort Study, Haiyan Zhao, Man Li, Dan Wu, Shuohua Chen, Chenrui Zhu, Yulong Lan, Hongmin Liu, Yuntao Wu, Shouling Wu

Research outputs 2022 to 2026

BACKGROUND: Emerging evidence suggests a central role for inflammation in cardiac conduction disorder (CCD). It is unknown whether habitual physical activity could modulate the inflammation-associated risks of incident CCD in the general population. METHODS AND RESULTS: This population-based cohort was derived from the China Kailuan study, including a total of 97 192 participants without prior CCD. The end points included incident CCD and its subcategories (atrioventricular block and bundlebranch block). Systemic inflammation was indicated by the monocyte-to-lymphocyte ratio (MLR). Over a median 10.91-year follow-up, 3747 cases of CCD occurred, with 1062 cases of atrioventricular block and 2697 cases of bundle-branch …

Preparation Of A Chiral Hyperbranched Polymer Based On Cinchona Alkaloids And Investigation Of Its Catalytic Activity In Asymmetric Reactions, Rafiqul Islam, Mohammad Shahid Ullah, Abdus Salam, Shinichi Itsuno

Turkish Journal of Chemistry

Cinchona alkaloid-derived sulfonamides and ester dimers containing chiral hyperbranched polymers have been successfully synthesized and applied as catalysts in asymmetric reactions. Several hyperbranched polymers derived from cinchona alkaloids, incorporating sulfonamides and esters, were synthesized through Mizoroki–Heck coupling polymerization. These polymers were subsequently applied in enantioselective Michael addition reactions. As the prepared polymers are not soluble in frequently used organic solvents, they act as efficient catalysts in the enantioselective reaction of β-ketoesters to nitroolefins, achieving up to 99% enantioselectivity with good yields. The insoluble property allows them to better satisfy “green chemistry” requirements and be used several times without losing the …

Magnetic Solid-Phase Extraction Technique Based On Fe3o4@Coppy-Pth Nanocomposite For Extraction Of Cobalt, Chromium, And Nickel Prior To Determination By Micro-Sample Injection System-Flame Atomic Absorption Spectrometry In Alcoholic And Nonalcoholic Beverages, Meli̇ke Küçüksakalli, Qamar Salamat, Buket Ti̇reli̇, Şükrü Gökhan Elçi̇

Turkish Journal of Chemistry

A novel Fe3O4@coPPy-PTH nanocomposite-based sorbent was prepared via in-situ oxidative polymerization using Fe3O4 nanoparticles with spherical and flower-like morphologies of thiophene and pyrrole as the feedstocks. The synthesized nanocomposite utilized sensitive extraction and determination of the metal ions Co(II), Cr(III), and Ni(II) without a chelating agent, followed by a micro-sample injection system-flame atomic absorption spectrometer (MIS-FAAS). The composition and morphology of Fe3O4@coPPy-PTH nanocomposite were characterized by advanced spectroscopic and imaging techniques like SEM and FT-IR. SEM observations showed the size of Fe3O4 nanoparticles changed from 30 nm to 120 nm in diameter after the copolymer (PPy-PTH) coating. The Fe3O4@coPPy-PTH nanocomposite …

Identification Of Blood At Simulated Crime Scenes Using Silver Nanoparticles With Sers, Uğur Köroğlu, Necdet Sağlam, Uğur Tamer, Ramazan Akcan, İsmai̇l Hakki Boyaci, Eylül Evran

Turkish Journal of Chemistry

Analysis of substances and samples obtained from the crime scene are very important in solving forensic cases. In order to determine the variables involved in a crime and to expedite the investigation process, especially the rapid analysis of body fluids in a small quantity and within an environment containing diverse components is necessary. For this reason, it is of great importance to analyse biological fluids with rapid, non-chemical contamination, non-destructive, low-cost and accurate techniques. In recent years, with advancements in laser technology, spectroscopic methods have been introduced as an analytical technique in forensic medicine and chemical studies. This study focuses …

Impact Of Cyp1a2 Genotypes On The Ergogenic Effects And Subjective Mood States Of Caffeine Ingestion In Resistance-Trained Women, Jessica M Prather, Christine M Florez, Amie Vargas, Bella Soto, Audrey Ross, Abby Harrison, Ariane H Secrest, Darryn S Willoughby, Sydney Kutter, Lem W Taylor

Faculty, Staff and Students Publications

Caffeine’s metabolism is determined by CYP1A2 genotypes: AC/CC (SLOW) and AA (FAST). This trial evaluated CYP1A2 genotypes’ impact on exercise and cognitive effects in 36 resistance-trained females assessed under placebo (PL) and caffeine (6 mg/kg bw anhydrous caffeine-CAF) conditions, before ingestion and throughout the session. 23andMe® (San Francisco, CA, USA) determined genotypes using saliva. Data were analyzed using two-way RMANOVA and paired-samples t-tests (p < 0.05). A significant main effect for genotype existed for leg press repetitions to failure (RTF) for CAF (p = 0.038), with the FAST group performing more repetitions than the SLOW (p = 0.027). There was a significant condition x genotype interaction for the subjective outcome index score (p = …

Epcam-Targeted Betulinic Acid Analogue Nanotherapy Improves Therapeutic Efficacy And Induces Anti-Tumorigenic Immune Response In Colorectal Cancer Tumor Microenvironment, Debasmita Dutta, Ashique Al Hoque, Brahamacharry Paul, Jun Hyoung Park, Chinmay Chowdhury, Mohiuddin Quadir, Soumyabrata Banerjee, Arghadip Choudhury, Soumik Laha, Nayim Sepay, Priyanka Boro, Benny Abraham Kaipparettu, Biswajit Mukherjee

Faculty, Staff and Students Publications

Background: Betulinic acid (BA) has been well investigated for its antiproliferative and mitochondrial pathway-mediated apoptosis-inducing effects on various cancers. However, its poor solubility and off-target activity have limited its utility in clinical trials. Additionally, the immune modulatory role of betulinic acid analogue in the tumor microenvironment (TME) is largely unknown. Here, we designed a potential nanotherapy for colorectal cancer (CRC) with a lead betulinic acid analogue, named as 2c, carrying a 1,2,3-triazole-moiety attached to BA through a linker, found more effective than BA for inhibiting CRC cell lines, and was chosen here for this investigation. Epithelial cell adhesion molecule (EpCAM) …

The Complex Molecular Epileptogenesis Landscape Of Glioblastoma, Victoria Soeung, Ralph B Puchalski, Jeffrey L Noebels

Faculty, Staff and Students Publications

The cortical microenvironment surrounding malignant glioblastoma is a source of depolarizing crosstalk favoring hyperexcitability, tumor expansion, and immune evasion. Neosynaptogenesis, excess glutamate, and altered intrinsic membrane currents contribute to excitability dyshomeostasis, yet only half of the cases develop seizures, suggesting that tumor and host genomics, along with location, rather than mass effect, play a critical role. We analyzed the spatial contours and expression of 358 clinically validated human epilepsy genes in the human glioblastoma transcriptome compared to non-tumor adult and developing cortex datasets. Nearly half, including dosage-sensitive genes whose expression levels are securely linked to monogenic epilepsy, are strikingly enriched …

Junctophilin-2 Regulates Mitochondrial Metabolism, Sasha Z Prisco, Lynn M Hartweck, Felipe Kazmirczak, Jenna B Mendelson, Stephanie L Deng, Madelyn Blake, Satadru K Lahiri, Xander H T Wehrens, Kurt W Prins

Faculty, Staff and Students Publications

No abstract provided.

Comprehensive Technical Analysis Of A Kickboxing Fight In K1 Format Based On Observation, Marta Niewczas, Wojciech Wąsacz, Wiesław Chwała, Tomasz Pałka, Ewa Sobiło-Rydzik, Tadeusz Ambroży, Kristína Němá, Łukasz Rydzik

Baltic Journal of Health and Physical Activity

Introduction: Observation and specialized analysis of confrontations in combat sports are fundamental for introducing corrections in training programs and for modifying the individual technical-tactical profiles of athletes in these types of activities. These actions comprehensively assess the progress of sports activities, ultimately inspiring and guiding the direction of training in sports clubs. The aim of this study was to analyze and assess the level of the offensive structure of Kickboxing sport fights in the K1 format, in terms of global simulated sparring, in selected thematic sets. Materials and Methods: The research material consisted of a multimedia recording of 10 simulated …