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Synthesis And Photooxygenation Of 3-(P-Substituted Phenyl)-3a,8a-Dihydro-4h-Cyclohepta[D]Isoxazoles: Facial Selectivity, Mahi̇re Emel Olgun, Abdullah Menzek, Ertan Şahi̇n, Yasi̇n Çeti̇nkaya

Turkish Journal of Chemistry

Two 3-(p-substituted phenyl)-3a,8a-dihydro-4H-cyclohepta[d]isoxazoles were synthesized by 1,3-dipolar cycloaddition of the corresponding nitrile oxides with cycloheptatriene. Two endoperoxides were synthesized as facially selective and single products in high yields (93-95%) from the reactions of isoxazole derivatives with singlet oxygen. The exact configurations of the endoperoxide with a methyl group in the phenyl ring and the diol synthesized from it were confirmed by X-ray analysis. To elucidate the mechanism, the formation energy of the endoperoxide was investigated by simulations using the software package Gaussian 09 and density functional theory calculations via the M06-2X/6-311+G(d,p) level method in dichloromethane. The results were consistent with …

New Imidazolidindionedioximes And Their Pt(Ii) Complexes: Synthesis And Investigation Of Their Antitumoral Activities On Breast Cancer Cells, Emrah Karahan, Tuğba Gençoğlu Katmerli̇kaya, Emel Önal, Aydan Dağ, Ayşe Gül Gürek, Vefa Ahsen

Turkish Journal of Chemistry

Breast cancer is one of the most common types of cancer worldwide and has the most lethality ratio for females among all cancers. Although current cancer therapeutics have made considerable advancements, there is still room for improvement in terms of efficacy. Many anticancer drugs have a risk of causing serious adverse effects due to their non-specific cytotoxic effects on both tumor and healthy cells. New therapeutics might have a greater ability to kill cancer cells, reduce the volume of tumors, and improve overall therapy response rates. Herein, we report the efficient synthesis and characterization of three amphi vic-dioximes and their …

Rational Design Of Allosteric Macromolecular Switches For Specific Biosensing And Conditional Genetic Control, Chenggang Xi

McKelvey School of Engineering Graduate Student Theses & Dissertations

Living cells inherently perceive and react to environmental stimuli, enabling communication within and between communities and across biological kingdoms. To harness the power of these natural genetic regulatory systems, synthetic biology has expanded its toolkit, facilitating applications in biosensing and metabolic engineering. However, natural sensory elements often face challenges in rapidly differentiating and accurately reporting chemicals with high structural resemblance, particularly in complex biomedical or environmental samples. In this work, we have enhanced the performance of existing genetic circuits to remove signal crosstalk, achieving exclusive responses to single effectors, and we have implemented modular sensory elements to expand detection ranges …

Return To Play After Arthroscopic Management Of Rotator Cuff Tears In Professional Athletes Of Contact Sports, Gautam Yagnik, Patrick Szukics, Jacob Seiler, Jonas Ravich, Luis Vargas, John Zvijac, John Uribe

All Publications

Background: Rotator cuff tears are rare injuries in professional athletes who participate in contact sports, and limited data exist to guide players and team physicians regarding outcomes after surgical management.

Purpose: To report the outcomes and return-to-play rates of professional contact sport athletes who underwent arthroscopic management of rotator cuff tears.

Study design: Case series; Level of evidence, 4.

Methods: All professional athletes of contact sports who underwent arthroscopic management of a rotator cuff tear between 2002 and 2019 at the institution were identified. Patient information collected were age, sport, position, date of injury, date of surgery, and time to …

Cell-Based Medicinal Products: Progress And Perspectives, Lijian Hui, Xiang Yuan, Jingqi Wu

Bulletin of Chinese Academy of Sciences (Chinese Version)

Cell-based medicinal products are a breakthrough that has been proven to treat previously incurable diseases, making them a topic of global interest. This review delves into the current state and progress of cell therapy, highlighting the remarkable therapeutic effects of various types of cell-based medicinal products. We specifically explore the potential development of cell-based medicinal products in China, focusing on promoting clinical translation and commercialization. We propose necessary actions to achieve these goals, such as fostering cutting-edge basic research, innovative therapeutic approaches, scalable manufacturing processes, and policy changes.

Analysis Of Serum Exosome Metabolites Identifies Potential Biomarkers For Human Hepatocellular Carcinoma, Tingting Zhao, Yan Liang, Xiaolan Zhen, Hong Wang, Li Song, Didi Xing, Hui Li

Faculty, Staff and Student Publications

Currently, the clinical cure rate for primary liver cancer remains low. Effective screening and early diagnosis of hepatocellular carcinoma (HCC) remain clinical challenges. Exosomes are intimately associated with tumor development and their contents have the potential to serve as highly sensitive tumor-specific markers. A comprehensive untargeted metabolomics study was conducted using exosome samples extracted from the serum of 48 subjects (36 HCC patients and 12 healthy controls) via a commercial kit. An ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) strategy was used to identify the metabolic compounds. A total of 18 differential metabolites were identified using the non-targeted metabolomics approach of UPLC-QTOF-MS/MS. …

Epigenome-Wide Association Study Of Prostate Cancer In African American Men Identified Differentially Methylated Genes, Anders Berglund, Kosj Yamoah, Steven A. Eschrich, Rana Falahat, James J. Mulé, Sungjune Kim, Jaime Matta, Julie Dutil, Gilberto Ruiz-Deya, Carmen Ortiz Sanchez, Liang Wang, Hyun Park, Hirendra N. Banerjee, Tamara Lotan, Kathryn Hughes Barry, Ryan M. Putney, Seung Joon Kim, Clement Gwede, Jacob K. Kresovich, Youngchul Kim, Hui Yi Lin, Jasreman Dhillon, Ratna Chakrabarti, Jong Y. Park

School of Public Health Faculty Publications

Introduction: Men with African ancestry have the highest incidence and mortality rates of prostate cancer (PCa) worldwide. Methods: This study aimed to identify differentially methylated genes between tumor vs. adjacent normal and aggressive vs. indolent PCa in 121 African American patients. Epigenome-wide DNA methylation patterns in tumor DNA were assessed using the human Illumina Methylation EPIC V1 array. Results: Around 5,139 differentially methylated CpG-sites (q < 0.01, lΔβl > 0.2) were identified when comparing normal vs. tumor, with an overall trend of hypermethylation in prostate tumors. Multiple representative differentially methylated regions (DMRs), including immune-related genes, such as CD40, Galectin3, OX40L, and STING, were detected …

Socioeconomic Status, Physical Inactivity, And Bmi In Transitional Urban China: Contextualizing The Theory Of Fundamental Causes, Jun Xu, Fang Gong, Wei Zhao

International Journal of Physical Activity and Health

Drawing insights from the theory of fundamental causes of disease, this study examined the relationships between socioeconomic status (SES) and selected risk factors in transitional urban China. Specifically, under China’s distinct institutional and sociocultural environment, we examined how contextualized SES (i.e., party membership, hukou status, housing ownership, and subjective social status) and conventional SES indicators (i.e., education, income, and occupation) were associated with physical inactivity and body mass index (BMI). Utilizing the urban subsample of the 2017 Chinese General Social Survey (CGSS), we found that individuals with higher education, income, Communist Party membership, and urban hukou status had lower risks …

Engaging Vs. Non-Engaging Abutments: An In Vitro Study Evaluating Changes In Microgap And Screw Morphology, Fawaz M. Alzoubi, Mohammad Y. Sabti, Esra Alsarraf, Faris A. Alshahrani, Steven J. Sadowsky

Pacific Faculty Work

Background: The purpose of this study was to compare the microgap size between engaging (E) and non-engaging (NE) abutments and screw morphology changes between E and NE abutments using scanning electron microscopy (SEM) before and after cyclic loading (CL). Methods: Thirty-six implants were arranged into four groups as follows: Group 1, single units with E abutments; Group 2, single units with NE abutments; Group 3, three-unit fixed partial dentures with a hemi-engaging design; and Group 4, three-unit FPDs with two NE abutments. The microgap was evaluated using a stereomicroscope. SEM was used to qualitatively evaluate screw morphology. The specimens were …

Investigation Of Heavy Metal Composition And Associated Health Risks From Selected Groundwater Wells In Temeke, Dar-Es-Salaam, Justin M. Jeremiah

Tanzania Journal of Engineering and Technology (TJET)

Water quality continues to be the challenge to residents in Dar es Salaam due to various anthropogenic activities in the city. This study was conducted to assess groundwater quality by determining concentrations of heavy metals. Both probability and Nonprobability sampling techniques were used to collect samples from 40 groundwater wells in 24 wards of Temeke municipal and analysed using Microwave Plasma Atomic Emission Spectrometer (Agilent 4210 MP-AES) at the Geochemistry Laboratory of the University of Dar es Salaam. Results indicated varying heavy metal concentrations ranging from 0.71 ± 0.08 to 6.64 ± 0.21 mg/l for As, 0.02 ± 0.01 to …

Junctophilin-2 Regulates Mitochondrial Metabolism, Sasha Z Prisco, Lynn M Hartweck, Felipe Kazmirczak, Jenna B Mendelson, Stephanie L Deng, Madelyn Blake, Satadru K Lahiri, Xander H T Wehrens, Kurt W Prins

Faculty, Staff and Students Publications

No abstract provided.

The Complex Molecular Epileptogenesis Landscape Of Glioblastoma, Victoria Soeung, Ralph B Puchalski, Jeffrey L Noebels

Faculty, Staff and Students Publications

The cortical microenvironment surrounding malignant glioblastoma is a source of depolarizing crosstalk favoring hyperexcitability, tumor expansion, and immune evasion. Neosynaptogenesis, excess glutamate, and altered intrinsic membrane currents contribute to excitability dyshomeostasis, yet only half of the cases develop seizures, suggesting that tumor and host genomics, along with location, rather than mass effect, play a critical role. We analyzed the spatial contours and expression of 358 clinically validated human epilepsy genes in the human glioblastoma transcriptome compared to non-tumor adult and developing cortex datasets. Nearly half, including dosage-sensitive genes whose expression levels are securely linked to monogenic epilepsy, are strikingly enriched …

Bp1003 Decreases Stat3 Expression And Its Pro-Tumorigenic Functions In Solid Tumors And The Tumor Microenvironment, Maria Gagliardi, Rhonda Kean, Bingbing Dai, Jithesh Augustine, Michael Roberts, Jason Fleming, D. Craig Hooper, Ana Ashizawa

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Overexpression and aberrant activation of signal transducer and activator of transcription 3 (STAT3) contribute to tumorigenesis, drug resistance, and tumor-immune evasion, making it a potential cancer therapeutic target. BP1003 is a neutral liposome incorporated with a nuclease-resistant P-ethoxy antisense oligodeoxynucleotide (ASO) targeting the STAT3 mRNA. Its unique design enhances BP1003 stability, cellular uptake, and target affinity. BP1003 efficiently reduces STAT3 expression and enhances the sensitivity of breast cancer cells (HER2⁺, triple negative) and ovarian cancer cells (late stage, invasive ovarian cancer) to paclitaxel and 5-fluorouracil (5-FU) in both 2D and 3D cell cultures. Similarly, ex vivo and in …

Hydroxyurea In The Sickle Cell Disease Modern Era, Chazmyn Riley, Walter K. Kraft, Robin Miller

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

INTRODUCTION: Sickle cell disease is an inherited disorder characterized by hemoglobin S polymerization leading to vaso-occlusion and hemolytic anemia. These result in a variety of pathological events, causing both acute and chronic complications. Millions around the world are affected by sickle cell disease with predominance in sub-Saharan Africa. Hydroxyurea was the first drug approved for use in sickle cell disease to reduce the occurrence of painful crises and blood transfusions in patients with frequent, moderate to severe painful crises.

AREAS COVERED: With the development of new therapeutics, the role of hydroxyurea is evolving. This narrative review aims to provide clinical …

Evaluating The Communication And Accountability Of Nurses In The Clinic Setting, Emily Baarz

Walden Dissertations and Doctoral Studies

Research has demonstrated the importance of effective communication and its relationship to positive patient, provider, and organizational outcomes. In the local organization, nursing staff verbalized concerns regarding lack of professional communication that may contribute to a hostile work environment and affect the quality of patient care delivered. To address this concern, the organization implemented a staff education program focused on effective communication practices and level of accountability. The purpose of this quality improvement project was to determine if the educational intervention focused on effective communication practices and level of accountability increased the participants’ perceptions of communication and accountability among nursing …

Synthesis And Characterization Of Nial-Hydride Heterometallics: Perturbing Electron Density Within Al–H–Ni Subunits, Aleida G. Gonzalez, Fernando Gonzalez, Edgar De Leon, Kaitlyn M. Birkhoff, Sam Yruegas, Haoyuan Chen, Manar Shoshani

School of Integrative Biological & Chemical Sciences Faculty Publications

Heterometallic hydride complexes are of growing interest due to their potential to contribute to highly active insertion-based catalysis; however, methods to modulate electron density within this class of molecules are underexplored. Addition of ancillary ligands to heterotrimetallic NiAl2H2 species (1) results in the formation of heterobimetallic NiAl-hydride complexes with varying phosphine donors (2-(L)2). Incorporation of sigma donating ancillary ligands of increasing strength led to contractions of the Ni–Al distances correlated to a strengthening of a back donation interaction to the Al–H sigma antibonding orbital, most prominently present in 2-(PMe3)2. Demethylation of the aryl ether from 2-(PMe3)2 provides access to a …

Identifying The Interactors Of Otud6b-2 That Are Important For Its Function On C-Myc, Farah Albatsh

Master's Theses

The complexity of molecular signaling pathways, particularly in cancer research, has made the proto-oncogene c-Myc a key focus due to its role as a master regulator of cellular processes such as growth, division, and apoptosis. Dysregulation of c-Myc is linked to various cancers, making it a highly sought therapeutic target despite challenges in directly inhibiting its function. Recent efforts have shifted towards indirect targeting methods, including BET inhibitors, cyclin-dependent kinase inhibitors, and siRNA formulations. A novel approach using PROTACs and the investigation of c-Myc-specific deubiquitinases (DUBs) has shown potential, particularly the emerging player OTUD6B-2. This study aims to identify and …

Physical Activity Modifies The Risk Of Incident Cardiac Conduction Disorders Upon Inflammation: A Population-Based Cohort Study, Haiyan Zhao, Man Li, Dan Wu, Shuohua Chen, Chenrui Zhu, Yulong Lan, Hongmin Liu, Yuntao Wu, Shouling Wu

Research outputs 2022 to 2026

BACKGROUND: Emerging evidence suggests a central role for inflammation in cardiac conduction disorder (CCD). It is unknown whether habitual physical activity could modulate the inflammation-associated risks of incident CCD in the general population. METHODS AND RESULTS: This population-based cohort was derived from the China Kailuan study, including a total of 97 192 participants without prior CCD. The end points included incident CCD and its subcategories (atrioventricular block and bundlebranch block). Systemic inflammation was indicated by the monocyte-to-lymphocyte ratio (MLR). Over a median 10.91-year follow-up, 3747 cases of CCD occurred, with 1062 cases of atrioventricular block and 2697 cases of bundle-branch …

Structural Basis For Substrate Binding And Selection By Human Mitochondrial Rna Polymerase, Karl Herbine, Ashok Nayak, Dmitry Temiakov

Department of Biochemistry and Molecular Biology Faculty Papers

The mechanism by which RNAP selects cognate substrates and discriminates between deoxy and ribonucleotides is of fundamental importance to the fidelity of transcription. Here, we present cryo-EM structures of human mitochondrial transcription elongation complexes that reveal substrate ATP bound in Entry and Insertion Sites. In the Entry Site, the substrate binds along the O helix of the fingers domain of mtRNAP but does not interact with the templating DNA base. Interactions between RNAP and the triphosphate moiety of the NTP in the Entry Site ensure discrimination against nucleosides and their diphosphate and monophosphate derivatives but not against non-cognate rNTPs and …

Thermal Regimes Of Beaver Impacted Stream Reaches In The Tualatin River Basin, Miles Mayer

Dissertations and Theses

Climate change and urbanization are expected to cause major changes to hydrologic regimes over the Pacific Northwest Region. The Tualatin River Basin, an area of the Pacific Northwest just west of Portland, OR has been subjected to considerable water-quality issues, including stream temperature. As ecosystem engineers, beavers have the potential to mitigate stream degradation and, consequently, there is increasing interest in beaver-related restoration. However, different conclusions have been found on beaver dam impacts to stream thermal regimes. These differences have been attributed to geographical differences in study location and variability in methods and investigated spatiotemporal scales. We investigated summer thermal …

Data From: Sheared Turbulent Flows And Wake Dynamics Of An Idled Floating Tidal Turbine, Lilian Lieber, Alex Nimmo Smith

Faculty of Science and Engineering Datasets

Input data (minimally processed ADCP Matlab files) used to generate this study's results. The data is divided into two folders: 1) Broad-scale Transects and 2) Fine-scale Transects and 3), a README file is provided.

Informatics-Based Discovery Of New Natural Products, Their Biosynthesis, And Their Biological Roles, Ethan Older

Theses and Dissertations

In this work, genome mining and biosynthetic knowledge are applied for the discovery of new natural products, their biosynthetic pathways, and their promising biological activities and roles through the development of two unique new informatics-based approaches.

In chapter one, an overview of microbial natural product biological functions and biosynthesis is provided. The use of bioinformatics leveraging microbial natural product biosynthetic knowledge is also introduced. This chapter highlights the importance and need for developing innovative approaches to accessing microbial natural products, a vastly untapped source of new molecules with promising biological activities.

In chapter two, a correlational network linking the gene-encoded …

Draft Genomes Of Neisseria Perflava Umb0578, Proteus Mirabilis Umb8339, And Enterococcus Faecalis Umb7967 Isolated From Urine Samples, Alex Kula, Ali Khan, Megan Martinez, Jevan Terry, Helen Appleberry, Alan J. Wolfe, Catherine Putonti

Biology: Faculty Publications and Other Works

The urinary tract of females harbors a variety of microorganisms, both for those with and without symptoms. Here, we present the draft genome sequences of three isolates from urine samples—Neisseria perflava UMB0578, Proteus mirabilis UMB8339, and Enterococcus faecalis UMB7967.

Draft Genomes Of One Staphylococcus Haemolyticus And Five Staphylococcus Lugdunensis Strains Isolated From Catheterized Urine Samples Of Females, Helen Appleberry, Haaris Anjum, Taleah Cage, Kayla Jarm, Haashir Khan, Lizzie Proctor, Junelle Saroca, Alan J. Wolfe, Catherine Putonti, Alex Kula

Biology: Faculty Publications and Other Works

Although Staphylococcus haemolyticus and Staphylococcus lugdunensis are members of the normal human flora, they also can cause infection. Here, we present the draft genomes of five strains of S. lugdunensis and one strain of S. haemolyticus isolated from transurethral catheterized urine samples from different females experiencing lower urinary tract symptoms.

Investigations Of Cell Tower Antennas Parameters On Reduction Of Radio Frequency Radiation Levels From Radio Base Stations, Florence U. Rashidi

Tanzania Journal of Engineering and Technology (TJET)

The widespread deployment of mobile cellular base stations in populated areas has raised public health concerns due to increased exposure to radio frequency (RF) radiation emissions. Exposure to high levels of RF radiation can have potential thermal and non-thermal biological effects. Optimizing the configuration of cell tower antenna parameters is crucial for mitigating these radiation levels. This study therefore aims at systematically investigating the influence of different cell tower antenna parameters on reducing the RF radiation levels from mobile base stations. Field measurements were conducted at two cell sites shared by multiple mobile operators. Electric field strengths were measured at …

Synergetic And Orthogonal Switching In Dual Photochromic Metal-Organic Frameworks, Gina Rene Wilson

Theses and Dissertations

Cooperative behavior and orthogonal responses of two classes of coordinatively integrated photochromic molecules towards distinct external stimuli were demonstrated on the first example of a photo-thermo-responsive hierarchical platform. Synergetic and orthogonal responses to temperature and excitation wavelength are achieved by confining the stimuli-responsive moieties within a metal-organic framework (MOF), leading to the preparation of a novel photo-thermo-responsive spiropyran-diarylethene based material. Synergistic behavior of two photoswitches enables the study of stimuli-responsive resonance energy transfer as well as control of the photoinduced charge transfer processes, milestones required to advance optoelectronics development. Spectroscopic studies in combination with theoretical modeling revealed a nonlinear effect …

Development Of A Urothelial Cell Line Expressing A Caspase-1 Biosensor To Measure Cellular Inflammatory Responses To Potential Uropathogens, Nikita Riya Kesav

Master's Theses

Urinary tract infections are one of the most common bacterial infections, historically implicating Escherichia coli as the causative pathogen. However, it is now known that there are a variety of bacteria that exist within the urinary tract, acting as either beneficial uroprotectives or opportunistic uropathogens. Furthermore, there are significant gaps in our understanding as to how these bacteria interact with the urothelium and what role each plays in maintaining or disrupting homeostasis. Research has shown that Toll-like receptor 4 (TLR4) signaling and Nod-like Receptor Protein 3 play a role in inflammasome activation during infection, triggering caspase-1 activation and inducing release …

Evolutionary Patterns Of Anolis Lizard Skulls: An Investigation In Geometric Morphometrics And An Educational Exploration Of Structure And Function, Katherine Beyer Starr

Master's Theses

This study explores the evolutionary patterns of Anolis lizard skulls and integrates findings into a curriculum focused on elucidating the structure and function of a lizard's head. The first objective was to develop an understanding of how the evolutionary patterns, of an extraordinary group of lizards (Anolis) compares to their relatives. Most of the prior research on Anolis morphology has focused on the post-cranium because of its significance towards subdivision of the arboreal habitat. But the group’s remarkable diversity in head shape has been seriously under-investigated. By performing geometric morphometric analysis of skull shape across a large sample of anoles …

Regulation Of Sterile Inflammatory Responses By Mitochondria-Associated Proteins, Meghana Venkatesan

Master's Theses

Inflammation is an important defense mechanism through which cells respond to pathogenic insults and damage/stress signals. Immune cells mediate extracellular inflammatory responses by synthesizing and releasing small proteins called cytokines, which play critical roles in modulating these responses. Specifically, key pro-inflammatory cytokines such as IL-1beta are released upon the assembly and activation of a multi-protein intracellular complex called the inflammasome, the most well-studied of which is the NLRP3 inflammasome. Several proteins have been identified as important regulators of cellular stress responses, including vacuole membrane protein 1 (VMP1). The present research shows that VMP1-depleted cells exhibit exacerbated cellular inflammation and are …

Epcam-Targeted Betulinic Acid Analogue Nanotherapy Improves Therapeutic Efficacy And Induces Anti-Tumorigenic Immune Response In Colorectal Cancer Tumor Microenvironment, Debasmita Dutta, Ashique Al Hoque, Brahamacharry Paul, Jun Hyoung Park, Chinmay Chowdhury, Mohiuddin Quadir, Soumyabrata Banerjee, Arghadip Choudhury, Soumik Laha, Nayim Sepay, Priyanka Boro, Benny Abraham Kaipparettu, Biswajit Mukherjee

Faculty, Staff and Students Publications

Background: Betulinic acid (BA) has been well investigated for its antiproliferative and mitochondrial pathway-mediated apoptosis-inducing effects on various cancers. However, its poor solubility and off-target activity have limited its utility in clinical trials. Additionally, the immune modulatory role of betulinic acid analogue in the tumor microenvironment (TME) is largely unknown. Here, we designed a potential nanotherapy for colorectal cancer (CRC) with a lead betulinic acid analogue, named as 2c, carrying a 1,2,3-triazole-moiety attached to BA through a linker, found more effective than BA for inhibiting CRC cell lines, and was chosen here for this investigation. Epithelial cell adhesion molecule (EpCAM) …