Digital Commons Network^™

Impa1-Derived Inositol Maintains Stemness In Castration-Resistant Prostate Cancer Via Impdh2 Activation, Che-Chia Hsu, Guihua Wang, Chien-Feng Li, Xian Zhang, Zhen Cai, Tingjin Chen, Bo-Syong Pan, Rajesh Kumar Manne, Gagan Deep, Haiwei Gu, Yuzhuo Wang, Danni Peng, Vasudevarao Penugurti, Xiaobo Zhou, Zhigang Xu, Zhongzhu Chen, Ming Chen, Andrew J Armstrong, Jiaoti Huang, Hong-Yu Li, Hui-Kuan Lin

Faculty, Staff and Student Publications

Acquisition of prostate cancer stem cells (PCSCs) manifested during androgen ablation therapy (ABT) contributes to castration-resistant prostate cancer (CRPC). However, little is known about the specific metabolites critically orchestrating this process. Here, we show that IMPA1-derived inositol enriched in PCSCs is a key metabolite crucially maintaining PCSCs for CRPC progression and ABT resistance. Notably, conditional Impa1 knockout in the prostate abrogates the pool and properties of PCSCs to orchestrate CRPC progression and prolong the survival of TRAMP mice. IMPA1-derived inositol serves as a cofactor that directly binds to and activates IMPDH2, which synthesizes guanylate nucleotides for maintaining PCSCs with ARlow/- …

Intrapartum And 30-Day Postpartum Complications In Patients With Antenatal Covid-19 Infection: A Retrospective Cohort Study, Sriya Donthi, Jacqueline Kloos, Kelly S. Gibson, David C. Kaelber

Student Scholarship

Objective: The study was aimed at comparing intrapartum and postpartum outcomes between pregnant patients with and without antenatal COVID-19 infection using aggregated, deidentified electronic health record (EHR) data. Design and Setting: This retrospective cohort study included data from over 80 health care organizations within the TriNetX Analytics Research Network. Population: Individuals admitted for delivery from Jan 2020 to May 2023 were studied. Methods: We studied individuals with ICD-10 codes for delivery, COVID-19 diagnosis, and primary outcomes. We compared the incidence of adverse intrapartum and 30-day postpartum outcomes in those with and without antenatal COVID-19. Main Outcome Measures: The main outcomes …

Contrasting Responses Of Commercially Important Northwest Atlantic Bivalve Species To Ocean Acidification And Temperature Conditions, Teagan Mcmahon, Diana Thatcher, Branwen Williams, Alan Wanamaker, Brittany Jellison, Heidi Franklin, Katherine Guay, Nina M. Whitney, Joseph A. Stewart, Michèle Lavigne

Faculty Publications

Modern calcifying marine organisms face numerous environmental stressors, including overfishing, deoxygenation, increasing ocean temperatures, and ocean acidification (OA). Coastal marine settings are predicted to become warmer and more acidic in coming decades, heightening the risks of extreme events such as marine heat waves. Given these threats, it is important to understand the vulnerabilities of marine organisms that construct their shells from calcium carbonate, which are particularly susceptible to warming and decreasing pH levels. To investigate the response of four commercially relevant bivalve species to OA and differing temperatures, juvenile Mercenaria mercenaria (hard shell clams), juvenile Mya arenaria (soft shell clams), …

Theoretical And Numerical Investigation Of An Ultra-Broadband Near-Perfect Absorption In Auxetic Metamaterials, Md. Rezwan Ahmed, Oishi Jyoti,, Ferdous Farjana, Pritu P. Sarkar, Alok Kumar Paul, Md. Samiul Habib

Mechanical Engineering Faculty Publications

In this paper, we present and numerically investigate an auxetic metamaterial absorber based on vanadium dioxide (VO2), achieving more than 90% absorption of the incident terahertz (THz) waves between 4.15 THz to 8.43 THz with an average absorption of 98.4%. To our knowledge, the absorption bandwidth is higher than previously reported VO2-based absorbers. The proposed absorber contains two VO2 based resonator rings placed diagonally at the top of the dielectric substrate in such a way as to create an auxetic shape and provide more design space than the existing absorbers. Considering the vector nature of electromagnetic fields in three-dimensional space, …

Post-Artesunate Delayed Hemolysis In Pediatric Malaria Patients In The United States, Sesh A Sundararaman, Karen L Hanze Villavicencio, Brianne Roper, Ziyi Wang, Amy K F Davis, Jonathan A Mayhew, Michelle L Wang, Nina L Tang, Vijaya L Soma, Gail F Shust, Margaret E Feeney, Indi Trehan, Jill E Weatherhead, Chandy C John, Jeffrey S Gerber, Audrey R Odom John

Faculty, Staff and Students Publications

Post-artesunate delayed hemolysis (PADH) occurred in 6 of 24 children treated with artesunate for severe malaria in the United States; however, severe hemolysis requiring hospitalization or transfusion was rare. In children in the United States treated with artesunate, counseling, and symptom monitoring may be preferred to weekly laboratory surveillance for PADH.

Initial Feasibility Cohort Of Temporally Modulated Pulsed Proton Re-Irradiation (Tmppr) For Recurrent High-Grade Intracranial Malignancies, Alonso Larosa, Zachary Fellows, Andrew Wroe, Len Coutinho, Eduardo Pons, Nicole Mcallister, Ranjini Tolakanahalli, Tugce Kutuk, Matthew Hall, Robert Press, Michael Mcdermott, Yazmin Odia, Manmeet Ahluwalia, Minesh Mehta, Alonso Gutierrez, Rupesh Kotecha

All Publications

Recurrent high-grade intracranial malignancies have a grim prognosis and uniform management guidelines are lacking. Re-irradiation is underused due to concerns about irreversible side effects. Pulsed-reduced dose rate radiotherapy (PRDR) aims to reduce toxicity while improving tumor control by exploiting dose-rate effects. We share our initial experience with temporally modulated pulsed proton re-irradiation (TMPPR), focusing on workflow, safety, feasibility, and outcomes for the first patient cohort. TMPPR was administered to patients with recurrent or progressive central nervous system malignancies using intensity modulated proton therapy with three fields. Patient and treatment data were collected, responses categorized using RANO assessment, and toxicities graded …

Deletion Of Fgfr2 In Ductal Basal Epithelium With Tamoxifen Induces Obstructive Meibomian Gland Dysfunction, Xiaowei Yang, Xingwu Zhong, Haotian Lin, Andrew J W Huang, Lixing W Reneker

2020-Current year OA Pubs

PURPOSE: Fibroblast growth factor receptor 2 (Fgfr2) is crucial for the homeostasis of meibomian gland (MG). However, the role of Fgfr2 in MG ductal epithelial progenitors remains to be delineated. Herein, we created a new transgenic mouse model with conditional deletion of Fgfr2 from MG ductal progenitors and investigated the cell-specific role in the pathogenesis of obstructive meibomian gland dysfunction.

METHODS: Peritoneal injection of tamoxifen (TAM) at 50 µg/gm for three consecutive days was performed to induce conditional deletion of Fgfr2 in two-month-old Krt5Fgfr2CKO or Krt5Fgfr2CKO-mTmG mice. Phenotypes of MG after Fgfr2 deletion were monitored by meibography, lipid staining, and …

Characterization Of Driver Mutations Identifies Gene Signatures Predictive Of Prognosis And Treatment Sensitivity In Multiple Myeloma, Jian-Rong Li, Abinand Krishna Parthasarathy, Aravind Singaram Kannappan, Shahram Arsang-Jang, Jing Dong, Chao Cheng

Faculty, Staff and Students Publications

In multiple myeloma (MM), while frequent mutations in driver genes are crucial for disease progression, they traditionally offer limited insights into patient prognosis. This study aims to enhance prognostic understanding in MM by analyzing pathway dysregulations in key cancer driver genes, thereby identifying actionable gene signatures. We conducted a detailed quantification of mutations and pathway dysregulations in 10 frequently mutated cancer driver genes in MM to characterize their comprehensive mutational impacts on the whole transcriptome. This was followed by a systematic survival analysis to identify significant gene signatures with enhanced prognostic value. Our systematic analysis highlighted 2 significant signatures, TP53 …

Transport Of Β-Amyloid From Brain To Eye Causes Retinal Degeneration In Alzheimer’S Disease, Qiuchen Cao, Shige Yang, Xiaowei Wang, Huaiqing Sun, Weijie Chen, Yuliang Wang, Junying Gao, Yanchi Wu, Qiuhua Yang, Xue Chen, Songtao Yuan, Ming Xiao, Maiken Nedergaard, Yuqing Huo, Qinghuai Liu

Faculty, Staff and Students Publications

The eye is closely connected to the brain, providing a unique window to detect pathological changes in the brain. In this study, we discovered β-amyloid (Aβ) deposits along the ocular glymphatic system in patients with Alzheimer's disease (AD) and 5×FAD transgenic mouse model. Interestingly, Aβ from the brain can flow into the eyes along the optic nerve through cerebrospinal fluid (CSF), causing retinal degeneration. Aβ is mainly observed in the optic nerve sheath, the neural axon, and the perivascular space, which might represent the critical steps of the Aβ transportation from the brain to the eyes. Aquaporin-4 facilitates the influx …

Nerve Injury Inhibits Oprd1 And Cnr1 Transcription Through Rest In Primary Sensory Neurons, Ashok Subedi, Asieh Etemad, Aadhya Tiwari, Yuying Huang, Biji Chatterjee, Samantha M Mcleod, Yungang Lu, Diangelo Gonzalez, Krishna Ghosh, Mario Sirito, Sanjay K Singh, Elisa Ruiz, Sandra L Grimm, Cristian Coarfa, Hui-Lin Pan, Sadhan Majumder

Faculty, Staff and Students Publications

The transcription repressor REST in the dorsal root ganglion (DRG) is upregulated by peripheral nerve injury and promotes the development of chronic pain. However, the genes targeted by REST in neuropathic pain development remain unclear. The expression levels of four opioid receptor genes (Oprm1, Oprd1, Oprl1 and Oprk1) and the cannabinoid CB1 receptor (Cnr1) gene in the DRG regulate nociception. In this study, we determined the role of REST in controlling their expression in the DRG induced by spared nerve injury (SNI). SNI induced chronic pain hypersensitivity in wild-type mice and was accompanied by increased levels of Rest transcript and …

Obesity And Outcomes In Adoptive Cellular Therapy In Solid Tumors, Derrick L Tao, Mirella Nardo, Cheuk Hong Leung, Heather Y Lin, Lei Kang, Hung Le, Ecaterina E Dumbrava, David S Hong

Faculty, Staff and Student Publications

This cohort study investigates the association of obesity with adoptive cell therapy outcomes in patients with solid tumors.

Strategies To Reduce Employee Turnover In The Insurance Industry In Jamaica, Kenneth Barrington Roberts

Walden Dissertations and Doctoral Studies

High employee turnover in insurance agencies can have a detrimental effect on overall team performance and significantly impact on the organization's financial health. To address the problem, insurance managers must understand effective strategies to improve employee retention. Grounded in Herzberg’s two-factor theory, the purpose of this qualitative pragmatic inquiry was to explore strategies insurance managers use to reduce their employee turnover rate. The participants were three insurance managers of the Jamaican insurance industry. Data were collected using semistructured virtual interviews and a review of public documents related to turnover data. Through thematic analysis, three themes emerged: a) supportive leadership for …

Federally Qualified Health Center Access To Maternity Care For Incarcerated Pregnant Women, Pamela Baldwin

Walden Dissertations and Doctoral Studies

Background: There has been an increase in pregnant incarcerated women, which is a growing concern for this high-risk and special population. This specialty group indicates a need for attention to ensure access to timely and proper medical care. Incarcerated pregnant women are a noted vulnerable group and do not have access to standardized clinical guidelines. Imprisonment imposes unique restrictions regarding access to care. A Federally Qualified Health Center (FQHC) in Pennsylvania has been providing ambulatory/obstetrics and gynecology (OBGYN) care to incarcerated pregnant women. Purpose: This research aimed to investigate strategies FQHC leadership can use to improve access to care for …

Cultural Responsivity And The Disproportionality Of African American Students In Special Education, Mia Hortman

Walden Dissertations and Doctoral Studies

The problem that was addressed in this study was a rural school district in southeastern Georgia was identified by the Georgia Department of Education as overidentifying African American students as having an intellectual disability (ID). The purpose of this study was to examine the perceptions of special education eligibility team members regarding this overidentification of African American students into the category of ID. This study was guided by Ladson-Billings’s (1995) theory of culturally responsive pedagogy. The research questions focused on the special education team members’ perceptions about the overidentification of African American students and their suggestions for a more culturally …

Actionable Structural Variant Detection Via Rns-Ngs And Dna-Ngs In Patients With Advanced Non-Small Cell Lung Cancer, Dwight Owen, Daniel Morgensztern, Et Al.

2020-Current year OA Pubs

IMPORTANCE: The National Comprehensive Cancer Network (NCCN) guidelines for non-small cell lung cancer suggest that RNA next-generation sequencing (NGS) may improve the detection of fusions and splicing variants compared with DNA-NGS alone. However, there is limited adoption of RNA-NGS in routine oncology clinical care today.

OBJECTIVE: To analyze clinical evidence from a diverse cohort of patients with advanced lung adenocarcinoma and compare the detection of NCCN-recommended actionable structural variants (aSVs; fusions and splicing variants) via concurrent DNA and RNA-NGS vs DNA-NGS alone.

DESIGN, SETTING, AND PARTICIPANTS: This multisite, retrospective cohort study examined patients sequenced between February 2021 and October 2023 …

Diagnosis And Treatment Of Giardia Lamblia Article Review, Wahda A. M. Khrofa, Ramzia H. A. Almizwary, Alyaa A. N. Alsaffo

BioMed Visions Journal

Giardia lamblia is a microscopic parasite belonging to the genus Giardia, renowned for its complex antigenic mechanisms. This parasite predominantly inhabits the small intestine, leading to a range of symptoms, including diarrhea, abdominal pain, and discomfort, affecting both humans and various animals such as dogs, cats, cattle, and sheep. The transmission of Giardia lamblia occurs through contaminated food, surface water, and fecal matter. The parasite spreads primarily through the fecal-oral route when hands contaminated with infected feces is exposed to the mouth, or when the infected cysts are ingested directly or indirectly.

Giardia lamblia goes through two primary life stages …

Subjective Deliberate Indifference: The Correct Standard For Pre-Trial Detainees' Fourteenth Amendment Claims For Inadequate Medical Care, Douglas Weeks

Pace Law Review

This article examines the legal standard of “subjective deliberate indifference” as it pertains to the Fourteenth Amendment claims of pre-trial detainees facing inadequate medical care. With the Supreme Court’s evolving interpretation of constitutional protections for incarcerated individuals, this piece argues that subjective deliberate indifference offers a more appropriate framework for evaluating the state’s duty to provide adequate medical treatment. By analyzing relevant case law, including the significant distinctions between pre-trial detainees and convicted prisoners, the article highlights the necessity of considering the mental state of correctional officials in medical negligence claims. Furthermore, it explores the implications of this standard on …

Samuel Alito's Dobbs Opinion And The Resurrection Of Second-Class Citizenship, Sue Mcgrath

Pace Law Review

This article examines Justice Samuel Alito’s opinion in the landmark Dobbs v. Jackson Women’s Health Organization case, analyzing its implications for women’s rights and reproductive freedom in the United States. By contextualizing Alito’s arguments within a historical framework, the piece argues that his decision represents a significant regression in civil rights, effectively resurrecting a form of second-class citizenship for women. The analysis delves into the legal reasoning employed, the socio-political ramifications of the ruling, and the broader implications for gender equality. Ultimately, the article calls for a critical reevaluation of judicial interpretations that prioritize certain rights over others, highlighting the …

On The Role Of Prediction In Streaming Hierarchical Learning, Ramy Mounir

USF Tampa Graduate Theses and Dissertations

In today's world, AI systems need to make sense of large amounts of data as it unfolds in real-time, whether it's a video from surveillance and monitoring cameras, streams of egocentric footage, or sequences in other domains such as text or audio. The ability to break these continuous data streams into meaningful events, discover nested structures, and predict what might happen next at different levels of abstraction is crucial for applications ranging from passive surveillance systems to sensory-motor autonomous learning. However, most existing models rely heavily on large, annotated datasets with fixed data distributions and offline epoch-based training, which makes …

Integrating Ecophysiology And Omics To Unlock Crop Response To Drought And Herbivory Stress, Insha Shafi, Manish Gautam, Rupesh Kariyat

Entomology Faculty Publications and Presentations

Introduction: Plants are constantly exposed to a diverse spectrum of environmental stressors that typically occur concurrently as multifactorial challenges, both spatially and temporally (Joshi et al., 2024). These stressors include abiotic stresses, such as drought, flooding, salinity, nutrient deficiencies, metal toxicity and extreme temperatures, as well as biotic pressures such as herbivory, pathogen attacks, and inter and intraspecific competition (Porter et al., 2020; González Guzmán et al., 2022). Drought and herbivory are arguably among the most critical factors that limit crop productivity in agricultural settings (Gautam et al., 2024), making it imperative to understand plant responses to their combined effects. …

Zooplankton Abundance, Composition, And Diversity And Associated Environmental Factors Across The Gulf Of Mexico And Along The Northwestern Coast Of Cuba, Shaniqua J. Gladney

USF Tampa Graduate Theses and Dissertations

My research focused on characterizing the zooplankton community of two regions in the Gulf of Mexico (GOM) along two transects, one across the middle of the GOM from Tuxpan, Mexico, to St. Petersburg, FL, and one along the northwestern coast of Cuba. Zooplankton bongo samples and associated environmental and physical processes were examined. These data were collected during two Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE) expeditions aboard the R/V Weatherbird II, during September 2015 (GOM) and May 2017 (Cuba). A Zooscan imaging system was used to identify and enumerate zooplankton taxa. Then zooplankton abundance, composition, and …

Does The Use Of Hydrotherapy And Behavioral Therapy In Addition To Pharmaceutical Medicine Reduce Chronic Back Pain In Adults?, Srujal Amin, Payton Golden

Master's Projects

Background: Chronic back pain (CBP) is a debilitating condition affecting millions worldwide, significantly impairing physical function, reducing quality of life, and imposing substantial healthcare costs. CBP is traditionally managed with pharmacological treatments, including opioids, acetaminophen, and nonsteroidal anti-inflammatory drugs (NSAIDs) which are often supplemented by prescribed physical activity to alleviate pain and improve quality of life. Recently, alternative therapies such as hydrotherapy and behavioral interventions have become more popular as safe and effective approaches to improving pain management in CBP patients. This integrative review aims to provide valuable insights into pain management, supporting healthcare professionals in developing more effective holistic …

Using The Conditional Maximum Likelihood Function With The Genetic Algorithm To Estimate Starima Models (Pλ,D,Qm), Ali Tareq Abdulmajeed, Firas A. Mohammed Almohana

Journal of Economics and Administrative Sciences

Strategic planning for a specific phenomenon depends mainly on accurate prediction by developing a model to represent that phenomenon. Therefore, this research dealt with spatiotemporal series models such as the STARIMA model (pλ, d, qm) model when there is a spatial correlation for neighbouring sites and a spatial correlation for the same geographical location. The methods for estimating the parameters are the Conditional Maximum Likelihood method and the method of estimating the parameters using the genetic algorithm (MLEGA). These models were compared using the statistical comparison metrics (RMSE). The data represents the daily infections with the COVID-19 epidemic for (10) …

A Genetic Algorithm Approach For Improving The Probabilistic Inventory Model With The Continuous Review: A Practical Application In The Department Of Pharmacy, Ahmed Jamal Mohammed, Faris M. Alwan

Journal of Economics and Administrative Sciences

This study investigates to shed light on artificial intelligence techniques, specifically the genetic algorithm, to improving traditional solutions, as well as finding the best policy for the problem of probabilistic inventory with continuous review by finding the optimal reorder point and the optimal economic quantity to avoid the risk of stock out (shortage), reduce total costs, and reach the optimal solution with little time and effort. The research was conducted in the stores of the department of pharmacy of the Ninawa health department for the period from January 1, 2021, to January 1, 2023, on a sample of three drugs …

Hidradenitis Suppurativa And Peripheral Ulcerative Keratitis, Indira Acharya, Mohamed I Jalloh, Caroline D Trevisan, Christopher J Haas

Journal of Community Hospital Internal Medicine Perspectives

The co-occurrence of inflammatory eye disease and hidradenitis suppurativa (HS) is uncommon; ocular involvement typically manifests as anterior uveitis. In this report, we present a unique case of peripheral ulcerative keratitis (PUK) where both infectious and rheumatological workup was negative, leading us to consider the possibility of an association between this condition and severe HS. As far as we are aware, there have only been six documented cases of PUK associated with HS.

Sevelamer-Induced Gastrointestinal Mucosal Injury: A Critical Review For Clinicians, Lefika Bathobakae, Phenyo Phuu, Saif Yasin, Rammy Bashir, Jessica Escobar, Ruhin Yuridullah, Gabriel Melki, Mohamed Elagami, Yana Cavanagh, Walid Baddoura

Journal of Community Hospital Internal Medicine Perspectives

Sevelamer is a non-absorbable polymer used to treat hyperphosphatemia in individuals with end-stage renal disease (ESRD) undergoing hemodialysis. The deposition of sevelamer crystals in the gastrointestinal (GI) tract, especially in the colon, can cause mucosal inflammation, pseudopolyps, ulceration, ischemia, or necrosis. Owing to its rarity and lack of physician awareness, the actual incidence and prevalence of sevelamer-induced gastrointestinal mucosal injury (SIGMI) remain unknown. The current evidence is retrospective, in the form of observational studies. This systematic review of case reports provides an overview of SIGMI, with a focus on its etiology, signs and symptoms, pathogenesis, diagnosis, and management. Electronic databases, …

New Era – Same Perspective, Surendra Marur, Gillian Junker

Journal of Community Hospital Internal Medicine Perspectives

Brief history of JCHIMP, future direction and acknowledgement of our 2023 peer reviewers and sources of our authors.

2024-25 Women In Science Conference Schedule (Pdf), Lynn University

Women in Science Conference

2024-25 Women in Environmental Science Conference Schedule (PDF)

Tak1 Blockade Plus Azacitidine Induces Apoptosis, Augments Cytarabine, And Ablates Clonogenicity In Mll-Af9+ Human Aml Cell Lines, Austin P. Runde, Cameron Lewis, Peter Breslin Sj, Jiwang Zhang

School of Medicine

Acute myeloid leukemia (AML) encompasses a diverse group of cancers that originate in the blood-forming tissues of the bone marrow [1]. Aside from the PML-RARA⁺ subtype, AML carries a 5-year survival rate of 28% for patients 20+ years of age. AML is the most common cancer of the hematopoietic system and is slightly more common in biological males; the average age at diagnosis is 68 years. Standard frontline treatment for AML is a two-phase regimen of intensive chemotherapy (CTx) employing daunorubicin and cytarabine; etoposide may also be added in select regimens. Despite 60-70% of patients achieving complete remission (CR), …

Notes On North American Butterflies, 2, Harry Pavulaan, Janice E. Arndt

The Taxonomic Report of the International Lepidoptera Survey

New natural history elements and distribution records of several North American butterflies are reported. While diversity and distribution of butterflies in North America are commonly believed to be fully known, the findings presented here show that much is yet to be learned of our butterfly fauna.