Digital Commons Network^™

The Role Of Wt1 In Müllerian Duct Development, Jace Aloway

Dissertations and Theses (Open Access)

WT1 is a zinc finger transcription factor widely expressed in the urogenital system. Human mutations of WT1 lead to pediatric nephroblastoma as well as frequent differences of sex development (DSDs). Previous studies have suggested that WT1 acts as an activator for Amhr2, a necessary component of typical male differentiation. We used the mouse as a model to investigate the role of WT1 in sex development, where we deleted Wt1 in the Müllerian duct (MD) mesenchyme using a novel conditional null reporter allele, Wt1 flox-RFP. This allele utilizes the Cre-lox system to delete exons 8 and 9 of Wt1 …

Pharmacological Inhibition Of Lsd1/Kdm1a In Diffuse Midline Glioma Models And Impact On Kinase Signaling, Faith Angelina Hernandez, Faith Angelina Hernandez

Dissertations and Theses (Open Access)

Diffuse Midline Gliomas (DMG) occur in the midline structures of the brain such as the brainstem. DMG, a subset of pediatric high-grade gliomas (pHGGs), confers poor prognosis in children with survival rates below two years post-diagnosis. This is heavily due to limited treatment options as radiation and chemotherapy are only temporarily effective and surgery pose high risks. DMG are characterized by histone mutations typically occurring in histone 3.1 and 3.3 alleles, with about more than 80% of patients containing the H3K27 mutation. Recent publications, highlight epigenetic modulators as promising cancer-specific targets emphasizing their potential as a novel therapeutic strategy. Epigenetic …

Characterizing And Targeting The Genomic Consequences Of Atrx Deficiency In Glioma, Sharvari Dharmaiah

Dissertations and Theses (Open Access)

Mutational inactivation of histone chaperone ATRX (a-thalassaemia/mental retardation X-linked) represents a defining molecular feature in several cancers, including malignant glioma. As standard of care only leads to transient responses and poor outcomes, there is an unmet clinical need for developing new therapies that target ATRX-deficient glioma. Loss of ATRX gives rise to abnormal G-quadruplex DNA secondary structures at GC-rich sites of the genome, such as telomeric and pericentromeric regions, enhancing replication stress and genomic instability. These mutations are mutually exclusive with TERT promoter mutations, promoting the alternative lengthening of telomeres (ALT) pathway as a telomere maintenance mechanism in ATRX-deficient glioma. …

When Is It Convenient For Indigenous Women To Influence State Policies?, Alaide Vences Estudillo, Norma Don Juan Pérez, Patricia Torres Sandoval, Fabiola Del Jurado Mendoza

Peace and Conflict Studies

This paper examines the difficulties that a network of Indigenous women in Mexico deal with when they seek peace for their people under a hybrid political structure. This paper discusses some distinctive aspects of the politics that these women engage in, how they conceive their political ideas, and how they use these ideas to structurally transform the colonial relationship that the liberal state maintains toward their people. They devise various strategies to influence state policies and to withstand the discrimination they face whenever they participate in democratic affairs.

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

The Magic Of Bioluminescence: How Animals Illuminate The Dark, Eleanor St. Clair

The Synapse: Intercollegiate science magazine

No abstract provided.

To Mine Or Not To Mine?: Discovery Of Polymetallic Nodules And Discussions On Deep-Sea Mining, Haze Doleys

The Synapse: Intercollegiate science magazine

No abstract provided.

The Bridge Newsletter Winter 2024, Missouri University Of Science And Technology

The Bridge Newsletter

• TED x Missouri S&T: Unveiling Resilience
• ElGawady named interim chair
• Ma named inaugural Kummer Impact Professor
• Sensor patent granted
• Burken awarded ASCE President’s Medal

Field Assessment Of Fertilization, Nursery, And Crop Management Practices Among Tomato (Solanum Lycopersicum L.) Growers In The Ilocos Provinces, Philippines, Annalissa Aquino, Sancho Bon, Angelica Guanlao, Ann Mylalulex Magnaye, Filomena Sta Cruz, Pompe C. Sta. Cruz

The Philippine Agricultural Scientist

Growth and yield of many economically important vegetables like tomatoes are heavily influenced by crop management practices, particularly fertilizer application, irrigation scheme, and pest management. To attain maximum tomato yields and increase farmers’ profits, these crop production components must be optimized in local farm levels. Before optimization measures can be conducted, baseline information on prevailing practices needs to be evaluated. To assess the nutrient management and other crop production practices of tomato farmers in the Ilocos provinces, Philippines, on-farm surveys were conducted in 16 and 18 barangays planting fresh-type and processing-type tomatoes, respectively. The study involved a total of 88 …

Hildegard Of Bingen’S Embryology: Enabling Women’S Reproductive Power Without Seed, Minji Lee

Department of Religion Faculty Scholarship and Creative Works

This paper examines Hildegard of Bingen’s embryological theory within the intricate landscape of medieval medicine and religion. It situates Hildegard’s understanding of conception within ongoing historical debates surrounding the roles of male and female reproductive substances, particularly the concept of seed or semen. By analyzing her conceptual framework against prevailing classical and medieval reproductive theories, the research illuminates Hildegard’s distinctive contribution to understanding conception as a holistic bodily process. Classical embryological discourse predominantly oscillated between Aristotle’s one-seed theory—which positioned men as sole generative agents—and Galen’s two-seed theory, which marginally acknowledged female reproductive participation. While technically aligning with Aristotelian principles by …

Ai Or Student Writing? Analyzing The Situational And Linguistic Characteristics Of Undergraduate Student Writing And Ai-Generated Assignments, Larissa Goulart, Marine Laísa Matte, Alanna Mendoza, Lee Alvarado, Ingrid Veloso

Department of Linguistics Faculty Scholarship and Creative Works

Since the release of OpenAI's ChatGPT, universities have faced the issue of whether there is still a place for written assignments in higher education. ChatGPT's capacity to mimic various written forms raises questions about the necessity of traditional assessments. Given this background, this study explores to what extent AI-generated assignments can replicate the situational and linguistic features of student-authored assignments. Using a corpus of undergraduate assignments from an English as a Foreign Language (EFL) context, we compare student responses with ChatGPT's outputs. Employing a register approach, we analyze the situational and linguistic characteristics of texts across three different registers—essays, critiques, …

Impact Of Human Immunoglobulin Genetic Variants On The Expressed Antibody Repertoire., Eric Engelbrecht

Electronic Theses and Dissertations

B cells produce immunoglobulins (IGs; antibodies), which are critical protein components of the immune system. Human IGs are composed of two pairs of identical ‘heavy’ chains and ‘light’ kappa or lambda chains, encoded by genes located at three loci in the genome: the IG heavy chain locus (IGH, chromosome 14), and the IG lambda (IGL, chromosome 22) and kappa (IGK, chromosome 2) loci. Each locus spans ~920-100 kilobase pairs (Kbp) of DNA and harbors families of gene segments that are somatically rearranged during B cell development. The IG loci collectively harbor >180 functional genes, many of which are part of …

Cuisine As A Compass: Exploring The Therapeutic Benefits Of Community, Cooking, And More Within The Lgbtqia+ Population, Sera Shevchenko, Karen Park Otd, Otr/L, Bcp, Susan Macdermott Otd, Otr/L

Fall 2024 OTD Capstone Symposium

Background: The LGBTQIA+ population experiences unique barriers to occupation and well being. Anti-LGBTQ legislation encroaches on inclusive and affirming care affecting occupational access. Limited occupational participation and additional exhaustive adaptive strategies to engage in occupations safely have an emotional cost. This research study explores how food or mealtime occupations such as cooking, eating, and hosting can support LGBTQIA+ well-being. This research was made possible by LGBTQIA+ participants that are experts of their lived experience.

Methods: This mixed methods research used both qualitative and quantitative methods to gather data on the significance of food-based occupations, culture, mental health, and identity in …

Assessment Of Nephroprotective Properties Of Vitex Agnus Castus Extract In Cisplatin-Treated Wistar Rats: A Pilot Study, Aparna Tripathy, Babi Dutta, Archana Parampalli Raghavendra, Sudarshan Surendran

Open Access archive

Cisplatin (CP) is used to treat various solid tumors but is associated with nephrotoxicity, which varies with dose and duration. Vitex Agnus castus (VAC) berries, known for their anti-inflammatory and antioxidant properties, may alleviate CP-induced renal toxicity. To investigate the gender-specific responses to cisplatin-induced nephrotoxicity and evaluate VAC extract's nephroprotective effects. Four-month-old Wistar rats (n=36) (24 male, 12 female) were used. In phase 1, gender-based differences in CP-induced nephrotoxicity were assessed. The gender group with higher nephrotoxicity was selected for phase 2 to evaluate VAC's nephroprotective properties. Animals were randomly grouped as Normal Control (6 males & 6 females), CP …

Cold-Shock Proteome Of Myoblasts Reveals Role Of Rbm3 In Promotion Of Mitochondrial Metabolism And Myoblast Differentiation, Paulami Dey, Srujanika Rajalaxmi, Pushpita Saha, Purvi Singh Thakur

Open Access archive

Adaptation to hypothermia is important for skeletal muscle cells under physiological stress and is used for therapeutic hypothermia (mild hypothermia at 32 °C). We show that hypothermic preconditioning at 32 °C for 72 hours improves the differentiation of skeletal muscle myoblasts using both C2C12 and primary myoblasts isolated from 3 month and 18-month-old mice. We analyzed the cold-shock proteome of myoblasts exposed to hypothermia (32 °C for 6 and 48 h) and identified significant changes in pathways related to RNA processing and central carbon, fatty acid, and redox metabolism. The analysis revealed that levels of the cold-shock protein RBM3, an …

Harnessing Trichoderma Mycoparasitism As A Tool In The Management Of Soil Dwelling Plant Pathogens, Srishti Singh, Alok Kumar Singh, Bhubaneswar Pradhan, Sudipta Tripathi

Open Access archive

Maintaining and enhancing agricultural productivity for food security while preserving the ecology and environment from the harmful effects of toxicants is the main challenge in modern monoculture farming systems. Microbial biological agents can be a promising substitute for traditional synthetic pesticides to manage plant diseases. Trichoderma spp. are soil-dwelling ascomycete fungi and are common biocontrol agents against diverse phytopathogens. Trichoderma-based biocontrol techniques can regulate and control soil-borne plant diseases through mechanisms such as mycoparasitism, the production of antibiotics and hydrolytic enzymes, rhizo-sphere competence, the effective competition for available resources, induction of plant resistance and facilitation of plant growth. Numerous secondary …

A Genotyping Array For The Globally Invasive Vector Mosquito, Aedes Albopictus, Luciano Veiga Cosme, Margaret Corley, Thomas Johnson, Dave W. Severson, Guiyun Yan, Xiaoming Wang, Nigel Beebe, Andrew Maynard, Mariangela Bonizzoni, Ayda Khorramnejad, Ademir Jesus Martins, José Bento Pereira Lima, Todd P. Livdahl

Biology

Background: Although whole-genome sequencing (WGS) is the preferred genotyping method for most genomic analyses, limitations are often experienced when studying genomes characterized by a high percentage of repetitive elements, high linkage, and recombination deserts. The Asian tiger mosquito (Aedes albopictus), for example, has a genome comprising up to 72% repetitive elements, and therefore we set out to develop a single-nucleotide polymorphism (SNP) chip to be more cost-effective. Aedes albopictus is an invasive species originating from Southeast Asia that has recently spread around the world and is a vector for many human diseases. Developing an accessible genotyping platform is essential in …

How Many Tiny Snails? Circumscription Of Three Species Of Pyrgulopsis (Mollusca: Gastropoda: Hydrobiidae)In Nevada Mountain Springs, Trenton Meadows

Theses and Dissertations

The springsnail genus Pyrgulopsis is one of the most diverse groups of freshwater gastropods in North America (Hershler, 2014), with many species displaying high levels of endemism (Hurt, 2003). This study attempts to circumscribe three putative species of springsnails: Pyrgulopsis serrata, Pyrgulopsis marcida, and a newly identified clade, referred to as Lineage C, from the Ruby Lake National Wildlife Refuge, Nevada, USA and nearby springs. Using an integrative taxonomic approach, we combined morphological, anatomical, and molecular data to examine species boundaries and evolutionary relationships.

Many lines of evidence were considered to determine species boundaries. Phylogenetic analysis of the mitochondrial …

Density Functional Theory And Machine Learning Analysis Of C—C Reductive Coupling From Terminal Cyanido And Cyaphido Complexes, Roberto Magdiel Escobar

Theses and Dissertations

Carbon–carbon (C–C) bond activation has gained increased attention as a direct method for the synthesis of pharmaceuticals and industrial chemistry. Due to the thermodynamic stability and kinetic inaccessibility of the C–C bonds, however, activation of C–C bonds by homogeneous transition-metal catalysts under mild homogeneous conditions is still a challenge. Most of the systems in which the activation occurs either have aromatization or relief of ring strain as the primary driving force. The activation of unstrained C–C bonds of phosphaalkynes does not have this advantage. This study employs Density Functional Theory (DFT) calculations using the Gaussian16 package to elucidate and compare …

Feasibility Study On Microwave Glucose Level Monitoring, Ricardo Cepeda

Theses and Dissertations

Diabetes management heavily relies on regular blood glucose monitoring, which traditionally involves invasive techniques. In recent years, significant research has been directed towards developing non-invasive glucose monitoring methods, particularly using electromagnetic waves. This study explores the feasibility of detecting blood glucose levels non-invasively by measuring the resonant frequency shifts of an antenna, influenced by the dielectric properties of blood. Microwave measurement techniques have garnered attention due to their ability to safely penetrate human tissue and detect biochemical markers like glucose. While previous studies have established a link between glucose concentration and dielectric properties, many techniques suffer from poor sensitivity or …

Synthesizing And Characterizing Some Pharmaceutical Resins Using Mannich Bases And Studying Their Biological Activity, Tamador Ali Mahmood

Baghdad Science Journal

Mannich bases functional groups were used, which is considered an inventive phenol formaldehyde resin was created in this study. The project was divided into two phases. The first portion involves creating a monomer of Mannich bases through a condensation reaction. The reaction occurs between aromatic aldehyde (P-hydroxy benzaldehyde) and primary amines like (Metoclopramide, trimethoprim, 2-aminobenzothiazoly, sulfamethoxazole, and 4-amino antipyrine) and with minor amines such as Carbazol. By condensing Mannich bases made in part one with formaldehyde and phenol, the second stage included making phenol formaldehyde resin with Mannich bases. The synthesized resins (Phenol-formaldehyde) have been characterized. The characterization was done …

The Positivity Principle: Investigating A School Administrator Training Series Designed To Promote Systemic Positivity, Catherine Gillach

Theses and Dissertations

Education has historically been recognized as a stressful profession, yet the Covid-19 pandemic (2020) paired with intensified socio-political influences have exacerbated educator stress, anxiety, and negativity, prematurely pushing more career educators to leave the profession. Teacher and principal pre-service preparation programs largely neglect to train educators in strategies for personally managing negative stressors, and negativity mitigation strategies are often overlooked as a critical facet of school climate and culture. Therefore, the purpose of this dissertation in practice was to examine the degree to which a school administrator training series could stimulate personal and systemic positivity within participants’ schools in a …

Rational Design Of Engineered Soft Materials For Mineral Scale Control: Synthesis, Characterization, And Performance Evaluation, Nadhem Ismail

Theses and Dissertations

Inorganic crystallization, referred to as mineral scaling, have been posing tremendous operational challenges to water-based industries over the years. Multiple recent initiatives have focused on developing sustainable and highly effective antiscalants/scale inhibitors; however, most of the investigated antiscalants have demonstrated low scaling ion tolerance and unknown scale inhibitor-mineral crystal interaction. Engineered soft materials, particularly Quantum Dots (QDs) and biopolymer-based formulations, are among the most desirable solutions since they both solve critical environmental issues and offer insights into the inhibition mechanism. This dissertation reports on the development and performance evaluation of three distinct soft materials-based formulations; Carboxyl-Silicon Quantum Dots (CSiQDs), Carboxymethylcellulose …

Evaluating Uas As A Tool For Improving Elk Population Counts In A Suburban Forested Area Of North Central Colorado, Michael Wussow

Theses and Dissertations

For decades, wildlife researchers have taken advantage of an aerial perspective for performing population counts because of challenges associated with ground observations. Due to advances in aviation technology and regulations, unoccupied aerial systems (UAS) have become increasingly available for wildlife population monitoring in addition to manned aircraft, with success largely dependent on the species, their habitat, and UAS utilized. This study compares a currently accepted method of counting North American elk (ground/spotting scope counts) to a novel method (UAS) to evaluate if a commercial-off-the-shelf quadcopter (COTS) UAS equipped with zoom-capable RGB cameras would be an acceptable tool for counting elk …

Researching Chlorophyll-Α Levels In Freshwater Lakes To Understand The Environmental State Of The Turtle Mountains Using Ndci Through Remote Sensing Techniques, Elliot Quinn Peltier

Theses and Dissertations

Chlorophyll α is one of the main indicators of water quality. Increasing levels have various triggers such as agricultural runoff, wastewater from treatment plants, sewage, and humus. Addressing chlorophyll α is crucial since high concentrations are precursors to eutrophication and are hazardous to human health. The Turtle Mountains in North Dakota and Manitoba is an island forest laden with lakes and trees. However, the noticeable extent of agricultural surface on the United States side of the border has made obvious the disintegration of forested area. Also, a lack of information and studies do not allow anyone to comprehend the level …

Using Problematic Sexual Behaviors And The Developmental Life Course Model To Predict Criminal Justice Involvement And Psychopathology By Early Adulthood, Amanda Schwartz

Theses and Dissertations

The developmental life course (DLC) model explains risk factors and developmental patterns among juveniles who have committed more than one sexual offense by considering the impact of major life events, experiences, and risk and protective factors throughout various developmental stages. Using an archival longitudinal dataset, the Longitudinal Studies of Child Abuse and Neglect (LONGSCAN), this study applied the DLC model to explore the relationship between gender and onset and persistence of problematic sexual behaviors (PSBs) in childhood and adolescence with criminal justice involvement and psychopathology by early adulthood (N = 330, 46% male, M = 18.26 years) after controlling for …

Empowering Community Leaders To Drive Sustainable Tourism And Small Business Development In Dangriga, Belize, Philip Nunez

Economics Undergraduate Honors Theses

This paper discusses the complexities of tourism development in Dangriga, Belize and how simple efforts can greatly impact small businesses in Central America.

The Effectiveness Of Mbsr On The Occupational Stress Of Correctional Officers, Nikolas Kerr

Dissertations

This dissertation looks at the negative effects of the work environment and other factors related to correctional officers lived experience. The intervention concentrates on the effect of mindfulness-based stress reduction on the occupational stress of correctional officers. Correctional officers have always dealt with difficult work conditions, but over the last 30 years the increase in the number of incarcerated people, the lack of retention among correctional officers, and the proliferation of inmates with complex mental health issues and gang affiliations have all created a much more complex and dangerous work environment. Combine this with the new understanding of the effects …

Enhancing Webtas: From An Atam Simulator To A Developmental Environment, Elizabeth G. Schmidt

Electrical Engineering and Computer Science Undergraduate Honors Theses

As interest in a niche research area, like the abstract Tile Assembly Model (aTAM), grows it is crucial to ensure that simulation tools are accessible and user-friendly for a broad audience. Existing resources can pose challenges for less experienced users, highlighting the need for enhancements that improve usability and functionality. To address these issues, key upgrades to the current online simulation platform, WebTAS, were implemented, including breakpoint functionality and nondeterminism checks, alongside features such as XML file integration, image capturing, and simulation view resets. These enhancements collectively elevate the platform from a basic learning tool to a comprehensive environment for …

Polarimetry Terahertz Imaging Of Human Breast Cancer Surgical Specimens, Nikita Gurjar, Keith Bailey, Magda O. El-Shenawee

Electrical Engineering and Computer Science Faculty Publications and Presentations

Purpose

We investigate terahertz (THz) polarimetry imaging of seven human breast cancer surgical specimens. The goal is to enhance image contrast between adjacent tissue types of cancer, healthy collagen, and fat in excised breast tumors. Based on the biological perception of random growth of cancer and invasion of surrounding healthy tissues in the breast, we hypothesize that cancerous cells interact with the THz electric field in a different manner compared with healthy cells. This difference can be best captured using multiple polarizations instead of single polarization.

Approach

Time domain pulsed signals are experimentally collected from each pixel of the specimen …