Digital Commons Network^™

Evaluating Osmia Cornifrons As An Effective Pollinator For Agricultural Crops: An Analysis Of Hair Morphology And Sex-Ratio Distorting Bacterium, Wolbachia, Nellie Heitzman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective pollination relies not only on the presence of pollinators but also on their morphological adaptations and reproductive biology. In this study, I investigated both the external hair morphology and the intracellular bacterial infection status of Osmia cornifrons, a solitary bee species increasingly used in orchard pollination due to its efficiency and early seasonal activity. Using Scanning Electron Microscopy (SEM), I characterized the distribution and structure of two major hair types present on the bee's body: branched and unbranched. Branched hairs were found widely distributed across the head, thorax, dorsal abdomen, and legs, and appeared to function primarily in incidental …

Individual And Collective Properties Of Tunable Photochemical Belousov-Zhabotinsky Micro-Reactors, Kudakwashe Benedict Shumba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cell-like model chemical systems are powerful tools that can be used to explore the role of intercellular coupling on population level behaviors in communities of biological cells. Firstly, we present a new method for fabricating such micro-reactors using the photosensitive Belousov–Zhabotinsky (BZ) reaction system employed in silica microparticles. These BZ micro-reactors have a tunable response to photochemical coupling, varying from a fully excitatory response to a fully inhibitory response. Their response can be tuned through variations in either the reactive mixture or, on an individual micro-reactor level, by changes in the synthesis temperature used during the fabrication of the silica …

Demonstrating The Potential Of Biophilic Design, Sustainable Site Initiatives, And Regenerative Landscape Design To Transform Neglected And Underutilized Spaces Into Vibrant, Eco-Friendly Hubs In Mckeesport Pennsylvania., Chinweuba Stephen Kikanme

Graduate Theses, Dissertations, and Problem Reports (ETD)

McKeesport is a city located along Monongahela and Youghiogheny rivers in Allegheny County, Pennsylvania, about 13 miles southeast of Pittsburgh. It is part of the River metropolitan area. McKeesport, once a thriving center of steel production, is now facing significant challenges associated with industrial decline, urban blight, flood hazard, population decline, brownfield sites and environmental degradation. However, the transformation of neglected spaces within the city into sustainable and vibrant urban hubs offers a powerful opportunity for revitalization. By incorporating natural elements such as green roofs, urban agriculture, and native plant restoration, this project seeks to create spaces that not only …

Leveraging Physiological Signal Activity And Self-Report Data To Assess Students’ Trust In “My Friendly Mind” App And Its Impact On Their Mental Health Knowledge: A Mixed-Method Phase 1 Clinical Trial Focusing On Depression And Attention Deficit Hyperactivity Disorder From Human Factors Standpoint., Yeganeh Shahsavar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mental health issues have become a significant global public health concern, especially among younger generations. The growing number of mental health challenges, combined with limited access to quality care, makes the problem even worse. Studies reveal that over 70% of individuals worldwide in need of mental health services do not receive appropriate care. Digital health technologies have the potential to enhance mental health services by making them more accessible and affordable. Despite the increasing popularity of mental health mobile applications (mHealth), there remains a lack of robust evidence of their effectiveness and the level of user trust, particularly in areas …

P23h Rhodopsin Accumulation Disrupts Synaptic Protein Levels In Rod Photoreceptors, Samantha Thompson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rod photoreceptors are specialized neurons located in the retina, the neural tissue that lines the back of the eye. Rod photoreceptors are necessary for dim light and peripheral vision through the photoactivation rhodopsin (Rho). Rho is synthesized in the inner segment of rods before being unidirectionally trafficked to the outer segment. In diseases such as retinitis pigmentosa (RP), Rho trafficking can be disrupted, leading to progressive rod cell death. In many cases of RP, Rho is mislocalized to the rod photoreceptor synapse (“spherule”). The impact of Rho mislocalization on spherule structure and protein organization is poorly understood. We hypothesize that …

Introduction Of Polar Functional Groups To Pyridinedipyrrolide Zirconium Photosensitizers Through Post-Synthetic Modifications, Marisa Nicole Tordella

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photosensitizers are vital components to applications such as photoredox catalysis, photodynamic therapy, and various avenues of solar energy production. The abilities of transition metal photosensitizers containing precious metals have been well studied over the years. However, because of the growing scarcity of these metals, there has been a shift in finding more earth abundant alternatives. To help fill this gap in the literature, the Milsmann group has developed and studied the photophysical properties of Zr(IV) complexes with various ligand systems. Due to the electron deficient metal center, these complexes exhibit ligand-to-metal charge transfer (LMCT), unlike the metal-to-ligand charge transfer commonly …

Investigating The Physiological, Metabolomic, And Transcriptomic Changes In Response To Telomerase Deletion In Saccharomyces Cerevisiae, Taizina Momtareen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Telomerase is an enzyme found in rapidly dividing cells that maintains chromosome ends and prevents the loss of genetic information during cell division. When telomerase is inactive, cells gradually lose their telomeres and eventually stop dividing. In yeast, a rare subset of cells can bypass this growth arrest by switching to a recombination-based mechanism to maintain telomeres. Therefore, yeast provides a powerful system to study this adaptation, as telomerase-deficient strains can generate 'survivors' that mimic the Alternative Lengthening of Telomeres (ALT) pathway observed in some human tumors. In this work, my goal was to examine how a telomerase-null (TLC1 …

Exploration Of Coping And Mental Health In West Virginia Farmers, Jodi Meadows Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Compared nationally, West Virginia currently ranks lowest for life expectancy, highest for drug related deaths and 35^th for access to adequate health (WV DHHR Organization Assessment & Strategic Plan, 2022). The state also ranks first in the nation for depression with one in four adults experiencing some form of depressive condition each year (CDC, 2020). The rurality of the state may limit social interaction and availability of medical treatment facilities (Brew et al., 2016).

The situation is even more dire for those in farming-related occupations. Farmers often struggle with management stress, utilizing appropriate coping strategies, and accessing mental health …

Police Abuse In America And The Search For Remedy, Howard Ryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The dissertation contends that, despite many diligent efforts—from mass protests to a presidential task force—America is not yet on path to remedying its outsized patterns of police abuse. The abuse problem is broadly characterized herein through a “triad” of over-enforcement, excessive force, and racial discrimination. The Washington Post’s “Fatal Force” database, covering the years 2015 through 2024, is offered as a bellwether of American progress in addressing its police abuse; and, it is observed, the nation’s yearly count of fatal police shootings of civilians has been increasing, rather than decreasing.

To help make sense of our lack of national progress, …

White Paper And Black Water: Environmental Control Of Labor In Appalachian Paper Mill Communities, Matthew C. Powell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In several small Appalachian communities, the West Virginia Pulp & Paper Company dominated the local landscape. The company stripped the mountainsides of spruce trees for pulpwood and used rivers for industrial processes and waste disposal. The small towns along these rivers and between these mountains relied on paper mills for employment. Corporate control over the natural environment, and water more specifically, was a necessary requirement for paper production, but also acted as a source of power over employees and their communities. When paper workers sought to organize, the company responded by leveraging their control over the environment against them to …

From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Situated in the Appalachian Mountains, one of the oldest mountain ranges on Earth, West Virginia waters boast rich ichthyofauna including native sportfish species walleye (Sander vitreus), largemouth bass (Micropterus nigricans), and muskellunge (Esox masquinongy). These three species are all native to the contemporary Ohio River watershed and play a major recreational and ecological role in local fisheries. Ecologically, all three species are apex predators and play a key role in ecosystems by directly influencing local fish assemblages. As dominant apex predators all three species are highly sought after in recreational fisheries with largemouth bass …

The Effect Of Carrier Type And Material Used On Results Of 1,1,1,2-Tetrafluoroethane Application To Teeth, Jonathon Logan Williams Dds

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The formulation of a pulpal diagnosis is crucial to prescribe appropriate treatment recommendations for endodontics. The application of a cold stimulus to the tooth’s surface utilizing the refrigerant 1,1,1,2-Tetrafluoroethane (R-134a) is a common way to obtain a pulpal diagnosis. Cotton as a form of applicator is used to absorb the R-134a and create a temperature change in the pulp. There is limited research if there is a better material than cotton to generate this cold response. No studies to date have been found to determine if a sponge as an applicator would generate a more significant cold response than …

Capillary Electrophoresis Analyses Using Ultraviolet Detection And Novel Mass Spectrometry Interfaces For The Characterization Of Small Molecules And Nanoparticles Of Pharmaceutical Interest, Yousef Shaaban Hassan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Capillary electrophoresis (CE) coupled to mass spectrometry (MS) offers a powerful analytical platform that unites high-efficiency separation with molecular-level identification. Despite its potential, limitations in interfacing and usability have constrained the widespread application of CE-MS. This thesis presents methodological advancements to enhance CE-based analysis through innovative interfacing, automation, and application to both small molecules and nanoparticle vaccine formulations. First, a nanoflow sheath CE-VSSI-MS interface is developed to overcome the limitations associated with flow-dependent ionization, enabling the use of narrow-bore capillaries, high separation voltages, and flexible pH conditions. This configuration effectively separated and detected diverse analytes, including β-blockers, amino acids, and …

Evidence-Based Lighting Design For Healthcare: Health Effects Of Timed Blue-Light At Night (Blan) On Night-Shift Staff, Seyedeh Nazli Hosseini

Graduate Theses, Dissertations, and Problem Reports (ETD)

The effects of light at night on night-shift clinical staff in healthcare settings is a topic of growing significance, considering the widespread adoption of artificial lighting and the increasing prevalence of night-shift work in the healthcare industry. The intersection of neuroscience and architecture provides a unique lens to explore the intricate relationship between lighting conditions and the circadian hygiene of night-shift medical staff. This project investigates the strategies associated with light exposure schedules for staff during night-shift work in healthcare settings.

Exposure to artificial light at night may contribute to mood disturbances and sleep disorders and consequently affect alertness and …

Monitoring West Virginia Wastewater For Norovirus: A Test Case For Tracking Foodborne Illness Outbreaks In Rural Communities Using Wastewater, Haley M. Phillippi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Foodborne illnesses are a global public health challenge. Foodborne illnesses, like norovirus, often have a large disease and societal burden. Norovirus is a single-stranded, positive-sense RNA virus, with 10 identified genogroups (GI-GX). Genogroups GI and GII are responsible for nearly all human cases of acute gastroenteritis worldwide. In the United States, HuNoV is not notifiable case-by-case. Instead, outbreaks are reported to national surveillance systems when two or more cases are epidemiologically linked. Most norovirus outbreaks occur during Winter to early Spring, however, certain individuals may still shed and transmit the virus without being detected or reported. Wastewater monitoring is a …

Filling Research Gaps In Occupational Health In Thermal Spray Coatings: A Scoping Review, Lab Characterization, And Lung Deposition Of Cold Sprayed Emissions, Bailley N. Reggetz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Thermal Spray Coating (TSC) processes are industrial surface treatment methods used to create coatings for repair, protection, and performance enhancement of components exposed to wear, corrosion, heat, or other harsh conditions. The most commonly used TSC techniques (flame spraying, electric arc spraying, HVOF spraying, plasma spraying, and cold spraying) are used across numerous industries including aerospace, automotive, biomedicine, and energy due to their ability to enhance surface properties such as providing corrosion resistance, wear resistance, and thermal insulation. While TSC techniques have been commercially available for nearly a century, they have undergone continuous development to accommodate more specialized applications, unique …

Revitalizing The Urban Riverfront: Redesigning The West End Bridge In Pittsburgh,Pa For Sustainable Multi-Use Connectivity And Public Space Creation., Mina Kargari Rezapour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban waterfronts are critical assets for cities, offering opportunities for ecological restoration, public engagement, and multi-modal transportation. However, many riverfronts remain underutilized due to infrastructure barriers and lack of accessibility.

Pittsburgh’s riverfront is dynamic asset that supports recreation, cultural expression, and community interaction. The Three River Heritage Trail Loop, a visionary project led by River Life over the past 20 years, seeks to connect diverse neighborhoods and natural assets along the city’s rivers. At the heart of this initiative is the West End Bridge, serving as the bookend and a pivotal gateway to complete the loop and critical link for …

Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Viruses are among the most abundant biological entities on Earth, yet their diversity and ecological roles in aquatic vertebrates, particularly fishes, remain poorly characterized. This dissertation advances fish virology through integrative approaches that combine historical context, molecular diagnostics, and genomic discovery. A novel hepadnavirus (ApHBV) was identified in asymptomatic Alosa pseudoharengus, emphasizing the prevalence of subclinical infections in migratory forage species. In Catostomus commersonii, transcriptomic profiling revealed sexually dimorphic immune responses to hepatitis B and C-like viruses, highlighting complex host-pathogen dynamics. In Micropterus nigricans, next-generation sequencing uncovered a novel nackednavirus and multiple adomaviruses within hyperpigmented lesions, expanding …

Neurobiological Variables Mediating The Relationship Between Breast Cancer And Sleep Disruption, Brittany Domenique Elliott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neurobiological Variables Mediating the Relationship between

Breast Cancer and Sleep Disruption

Brittany D. Elliott, MS

Breast cancer is the most commonly diagnosed malignancy in women, with more than two million new cases diagnosed world-wide each year. Improvements in screening technology and diagnostics have increased survival rates, but cancer-associated sleep disruptions persist for the majority of breast cancer survivors well beyond the treatment period. In addition to reducing quality of life, reduced sleep quality is associated with greater incidences of affective dysfunction, reduced treatment efficiency, aggressive cancer progression, increased symptom severity, and increased mortality by all causes. Thus, identifying the variables …

Non-Lethal Sampling Methods For Fish Health Assessments Using Smallmouth Bass Micropterus Dolomieu, Lindsey Nicole Hartzell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the early 2000s, fish kills of different species, including smallmouth bass Micropterus dolomieu, were reported in areas of the Potomac River drainage, including the Shenandoah River in Virginia. Since the first reports of fish kills and lesions affecting smallmouth bass, they have continued to show signs of adverse health effects. Additionally, in some areas of the Potomac River drainage, population declines have occurred, and lethal sampling is discouraged. To develop a holistic, integrative approach to assess fish health, Smallmouth Bass were collected and sampled using non-lethal techniques at three sites within the Shenandoah River drainage, VA and one …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Development And Applications Of Capillary Vibrating Sharp-Edge Spray Ionization Coupled With Atmospheric Pressure Chemical Ionization (Cvssi-Apci), Madison Elizabeth Pursell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mass spectrometry (MS) is a technique that is used in fields in which both complex and non-complex mixtures are analyzed. The state-of-the-art ion sources that are utilized for MS are being demonstrated to be functional for direct infusion of samples as well as for use with online separations. However, ion sources, such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) can encounter problems such as ion suppression and an inability to ionize based on size and/or polarity, respectively. Further these sources are bulky and require gas flow, heat sources, and additional high voltage power supplies for optimal performance. …

Microbial And Plant Biodiversity In Appalachian Agroecosystems And Their Implications For Soil Health, Kinsey Reed Close

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sustainability of agricultural landscapes depends on understanding the complex interactions between plants, soil, and microbes. In Appalachia, a region shaped by complex terrain and long histories of human and ecological interaction, small family farms produce hay, livestock, and row crops. These agroecosystems, though often treated as “artificial” by many ecologists, have the potential to support both food production and biodiversity conservation if managed with an understanding of ecological complexity. This dissertation examines how plant and microbial diversity interact with soil and management to influence nutrient cycling, soil health, and long-term resilience in Appalachian agricultural systems. A literature review revealed …

Dynamic Force Feedback Controller Improves Metrics Of Stepping In A Biomimetic Robot Leg, Isabella Mae Kudyba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Force feedback is a type of robotic control where forces are measured, fed back into the control system, and actuation is adjusted to compensate for environmental forces, with the goal of quick and precise limb positioning in walking. Robots of today use single force sensors (e.g. pressure sensitive pads, load cells) per leg which are often bulky, expensive, and susceptible to crosstalk. Walking robots often employ model predictive controllers or policy controllers trained through reinforcement learning (RL) to actuate their limbs, but these methods are time and energy consuming. Biomimetic robots offer an alternative approach, investigating biological theories with the …

Synergetic Effect Of Cellulose And Chitosan Polymers In Cancer Treatment, Grace Peterson

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research explores the development of a biocompatible, natural drug delivery system designed for targeted cancer therapy. Lung cancer cells are used in these particular tests, but further research indicates treatment could be used for various cancer types. Traditional cancer treatments like chemotherapy are often limited by poor selectivity for tumor cells, causing significant damage to healthy tissues. The study proposes a dual-layered delivery system, utilizing Carboxymethyl Cellulose (CMC) as the core material and a charge-reversible chitosan shell to encapsulate and target cancer drugs. CMC, with its water solubility and ability to form stable gels, serves as a stable drug …

Replicating The Functionality Of Ghost Knifefish Cerebellar Feedback Using Synthetic Nervous Systems, Sheldon Paul Cj Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory inputs allow animals to perceive, react, and adapt to an environment. However, the sensory information received by the body, such as visual, auditory, and proprioceptive information, could become overwhelming, thus overloading the brain. Yet, animals can process all this information by canceling redundant signals from their surroundings, allowing them to be more sensitive to novel or unexpected signals in their environment. Each species (i.e., birds, fish, mammals) has its own way of using and filtering sensory information, from auditory to locomotion adaptivity. Cerebellar circuits contribute to sensory filtering in a variety of systems. In particular, research on Ghost knifefish …

Rational Design Of Small Molecules Targeting Mitochondrial Redox Proteins, Matheus Barbosa Belchior

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reactive oxygen species play a crucial role in many cellular processes. Despite being a natural byproduct of cellular metabolism, overexpression of these species causes damage to the cell, leading to cell dysfunction. Central to the regulation of these highly reactive molecule is the mitochondria. Impairment in its function has been associated with oxidative stress within the cell. Herein, a ligand-based and structure-based approach to design new small molecules to inhibit mitochondrial redox proteins associated with oxidative stress and reactive oxygen species. In the first project two molecules were synthesized and characterized as potential monoamine oxidase B inhibitors. Results showed two …

Identifying The Role And Influence Of Interleukin-27 On The Neonatal Immune Response, Jordan Kane Vance

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first month of life is a critical and vulnerable developmental stage, characterized by a heightened risk of life-threatening infections. Each year, 1.3 million newborns succumb to neonatal bacterial sepsis. The immune system during the neonatal period has unique features that lead to inefficiencies at clearing infection. Early life immunity is characterized by a Th2-biased profile with decreased IL‑12, IFN-γ, and TNF-α, along with increased IL‑6 and IL‑23. Additionally, IL‑27, an immunosuppressive cytokine, is also elevated during this period and further contributes to susceptibility to infection. Early diagnosis and intervention are critical in administering effective care in newborns and there …

Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ability for beef cattle to utilize feed efficiently is a critical determinant for both economic and environmental sustainability in livestock production. However, despite extensive research, the complex microbial ecosystem within the rumen and its relationship to feed efficiency remain incompletely understood. This dissertation explores the physiological and microbial foundations of feed efficiency by characterizing how rumen microbial communities and their functions differ among beef cattle with divergent residual feed intake (RFI) phenotypes and how the RFI status of beef cattle influenced their response to dietary interventions such as supplementation with a Bacillus-based feed additive. In the first study, …

Weakly Reversible Deficiency Zero Realizations Of Polynomial Dynamical Systems, Neal Ryan Buxton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Weakly reversible, deficiency zero (WR0) systems form a large class of polynomial ODEs modeling chemical reaction networks. Their behavior is exceptionally stable: they have unique positive steady states, which are locally asymptotically stable (they are also conjectured to be globally asymptotically stable). This powerful result (the Deficiency Zero Theorem) applies to a large class of high dimensional, nonlinear polynomial dynamics, and is independent of the choice of parameters in the model. In this dissertation we present two algorithms that expands the scope of the Deficiency Zero Theorem to:

1. Networks with WR0 realizations, i.e. networks that are not necessarily WR0 …