Digital Commons Network^™

Non-Lethal Sampling Methods For Fish Health Assessments Using Smallmouth Bass Micropterus Dolomieu, Lindsey Nicole Hartzell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the early 2000s, fish kills of different species, including smallmouth bass Micropterus dolomieu, were reported in areas of the Potomac River drainage, including the Shenandoah River in Virginia. Since the first reports of fish kills and lesions affecting smallmouth bass, they have continued to show signs of adverse health effects. Additionally, in some areas of the Potomac River drainage, population declines have occurred, and lethal sampling is discouraged. To develop a holistic, integrative approach to assess fish health, Smallmouth Bass were collected and sampled using non-lethal techniques at three sites within the Shenandoah River drainage, VA and one …

The Effect Of Sars-Cov-2 On Bacterial Diversity And Abundance In Community Wastewater, Joshua Lambert

Graduate Theses, Dissertations, and Problem Reports (ETD)

Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) enters human respiratory cells through binding of its spike (S) protein to the ACE-2 receptor, which is highly expressed in lung cells, but is also found in over 150 other cell types encompassing major organs including GI enterocytes. SARS-CoV-2 causes gut dysbiosis in infected individuals and patients with COVID-19 and also coincides with lower alpha diversity of the individual’s gut microbiome. Certain gut microbiome-associated genera are lower in abundance in COVID-19 patients including Lachnospira, Prevetolla, Roseburia, Bifidobacterium, and Faecalibacterium while genera such as Bacteroides, Enterococcus and Proteobacteria are increased during a SARS-CoV-2 …

Dynamic Force Feedback Controller Improves Metrics Of Stepping In A Biomimetic Robot Leg, Isabella Mae Kudyba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Force feedback is a type of robotic control where forces are measured, fed back into the control system, and actuation is adjusted to compensate for environmental forces, with the goal of quick and precise limb positioning in walking. Robots of today use single force sensors (e.g. pressure sensitive pads, load cells) per leg which are often bulky, expensive, and susceptible to crosstalk. Walking robots often employ model predictive controllers or policy controllers trained through reinforcement learning (RL) to actuate their limbs, but these methods are time and energy consuming. Biomimetic robots offer an alternative approach, investigating biological theories with the …

Power, Economy, And Food Systems In The Confederacy: A Material History Of The Confederate Subsistence Department, John Martin Mcmillan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation questions how human and non-human actors share power. By exploring Confederate logistics within the Subsistence Department, the collaboration between bureaucrats and the material world is revealed. Contributing to scholarship aimed at expanding the field of Civil War studies, this dissertation not only provides a more complex and interesting view of the conflict, but also an understanding that human beings are not solely responsible for the war’s results. By applying a materialist approach, the causes and outcomes of an event as complicated as a civil war can be better explained and understood. The choice to stockpile food for distribution …

Identifying The Role And Influence Of Interleukin-27 On The Neonatal Immune Response, Jordan Kane Vance

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first month of life is a critical and vulnerable developmental stage, characterized by a heightened risk of life-threatening infections. Each year, 1.3 million newborns succumb to neonatal bacterial sepsis. The immune system during the neonatal period has unique features that lead to inefficiencies at clearing infection. Early life immunity is characterized by a Th2-biased profile with decreased IL‑12, IFN-γ, and TNF-α, along with increased IL‑6 and IL‑23. Additionally, IL‑27, an immunosuppressive cytokine, is also elevated during this period and further contributes to susceptibility to infection. Early diagnosis and intervention are critical in administering effective care in newborns and there …

Rational Design Of Small Molecules Targeting Mitochondrial Redox Proteins, Matheus Barbosa Belchior

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reactive oxygen species play a crucial role in many cellular processes. Despite being a natural byproduct of cellular metabolism, overexpression of these species causes damage to the cell, leading to cell dysfunction. Central to the regulation of these highly reactive molecule is the mitochondria. Impairment in its function has been associated with oxidative stress within the cell. Herein, a ligand-based and structure-based approach to design new small molecules to inhibit mitochondrial redox proteins associated with oxidative stress and reactive oxygen species. In the first project two molecules were synthesized and characterized as potential monoamine oxidase B inhibitors. Results showed two …

Ciliary Arl14b In Vision, Alexis Breann Crockett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary cilia are highly specialized organelles that regulate signaling, trafficking, and sensory perception across multiple tissues, including the retina. The small GTPase ADP Ribosylation Factor-Like Protein 13B (ARL13B) is an atypical ARF-family protein enriched in cilia. ARL13B acts as a guanine exchange factor (GEF) for another small GTPase ARF-like Protein 3 (ARL3). Mutations in ARL13B cause Joubert syndrome, a ciliopathy characterized by brain malformation, developmental delays, and retinal degeneration. Despite ARL13B’s known ciliary localization and clinical relevance, ARL13B’s precise functions in cilia remain poorly defined. This dissertation investigates the cilia-specific roles and GEF-specific roles of ARL13B in photoreceptors using two …

Sleep, Endocannabinoid Signaling, And Cortical Processing In Mouse Models Of Autism Spectrum Disorder, Bryan Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sleep plays a crucial role in memory consolidation, neuronal plasticity, and cognitive function. Individuals with autism spectrum disorder (ASD) often experience sleep disturbances, which are positively correlated with symptom severity. However, on the surface, sleep architecture is remarkably normal under baseline conditions in many rodent models of ASD. The goals if this dissertation were to investigate whether regulation of sleep, biochemical signaling during sleep, and downstream consequences of sleep on behavior are altered in two mouse models of ASD. In Aim 1, we tested the hypothesis that sleep phenotypes emerge in ASD models under conditions of high sleep pressure. We …

Weakly Reversible Deficiency Zero Realizations Of Polynomial Dynamical Systems, Neal Ryan Buxton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Weakly reversible, deficiency zero (WR0) systems form a large class of polynomial ODEs modeling chemical reaction networks. Their behavior is exceptionally stable: they have unique positive steady states, which are locally asymptotically stable (they are also conjectured to be globally asymptotically stable). This powerful result (the Deficiency Zero Theorem) applies to a large class of high dimensional, nonlinear polynomial dynamics, and is independent of the choice of parameters in the model. In this dissertation we present two algorithms that expands the scope of the Deficiency Zero Theorem to:

1. Networks with WR0 realizations, i.e. networks that are not necessarily WR0 …

Three Essays On The Socioeconomics Of Drinking Water Pfas Contamination, Nabin Babu Khanal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Per- and polyfluoroalkyl substances (PFAS) are a group of over 15,000 synthetic compounds widely used for their resistance to heat, stains, grease, and water. While central to many modern conveniences, ingestion of PFAS is increasingly linked to adverse human health outcomes and are now detected globally in drinking water systems. This dissertation investigates three interrelated aspects of PFAS contamination in U.S. drinking water: (1) socioeconomic determinants and spatial patterns of contamination; (2) the economic impacts of contamination on residential property values; and (3) the effect of inconsistent PFAS regulations on public trust in environmental governance.

Using data from the U.S. …

Identification, Mapping And Treatment Of The Invasive Rosa Multiflora Using Unmanned Aerial Systems And Machine Learning Models, Kylie Virginia Shaw

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species are introduced species that cause detrimental impacts to the native ecosystem or cause harm to human health. The work necessary for land managers to survey for and treat invasive species takes up limited time, labor, and financial resources. This study assesses how Unmanned Aerial Systems (UAS) and machine learning models can be used to make the identification, mapping, and treatment of invasive vegetation more efficient and effective. A UAS-mounted multispectral camera captured images of our target species, Rosa multiflora (multiflora rose), throughout its growing season in southwest Pennsylvania. A random forest machine learning model was trained on these …

Watershed-Level Survey Of Antimicrobial Resistance From Onsite Wastewater Systems, Trey Franklin Clark

Graduate Theses, Dissertations, and Problem Reports (ETD)

The emergence of antimicrobial resistance (AMR) has quickly become one of the largest global health and development threats. Research related to environmental modes of transmission of AMR has accelerated in recent years, and the US Environmental Protection Agency is particularly interested in this emerging contaminant. Knowledge of how onsite (decentralized) wastewater treatment systems contribute to this issue is lacking, so this research serves to analyze and quantify the environmental dissemination of AMR and fecal contamination from these sources at a watershed level while using statistical analysis to assess relationships between physiochemical, spatial, and sampling parameters. This research surveyed four watersheds …

Development Of Novel Carbohydrate-Based Nanoparticles For Precision Drug Delivery, Krystal Ann Hughes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoparticle-based drug delivery has made tremendous advancements in the last decade. However, several critical factors, including precision drug delivery at the site of interest, long-term storage stability, and controlling release profiles of drugs in the systemic circulation, need to be addressed for successful clinical translation of nanomedicine. This dissertation presents novel carbohydrate-based nanoparticles that achieve precise intracellular delivery and therapeutic release at the disease site. We present the formulation and optimization of polymeric and polymer-lipid hybrid nanoparticles that enable precision intracellular delivery and passive accumulation of therapeutics in the bone marrow. Intracellular delivery is highly impactful and sought after. One …

Saferespirator: Comprehensive Database For N95 Filtering Facepiece Respirator Leak Detection Including Infrared, Rgb Videos, And Quantitative Fit Testing, Geoffrey Marchais, Mohamed Arbane, Barthelemy Topilko, Jean Brousseau, Clothilde Brochot, Yacine Yaddaden, Ali Bahloul, Xavier Maldague

Études primaires

The COVID-19 pandemic underscored the challenges of performing mandatory Quantitative Fit Tests (QNFT) for healthcare professionals and the limitations of self-administered fit checks. To address this, it is crucial to develop faster and more efficient methods for detecting, locating, and quantifying leaks in Filtering Facepiece Respirators (FFRs), providing wearers with immediate feedback on their safety. Infrared (IR) technology, which relies on temperature variation analysis around the face seal, has proven effective for locating leaks but has not yet achieved automated quantification. This paper introduces a validated protocol for creating a comprehensive database to advance automatic leak detection. The database includes …

Mechanical And Antimicrobial Properties Of Green And Photoactive Agtio2/Poly(3hydroxybutyrate) (Phb) Electrospun Membranes, Safa Ladhari, Nhu Nang Vu, Alireza Saidi, Amine Aymen Assadi, Phuong Nguyen-Tri

Études primaires

The rise of antibiotic-resistant microbes and concerns over non-biodegradable waste highlight the need for innovative materials with integrated functionalities that address these critical issues. In this study, we synthesized poly(3hydroxybutyrate) (PHB) electrospun nanofibers blended with gelatin (Ge) and loaded with photoactive AgTiO2 nanoparticles with improved mechanical and biological properties. Biological tests revealed excellent antibacterial activity of the prepared membrane, with efficiency exceeding 99% against Escherichia coli and 95% against Staphylococcus epidermidis after 90 and 60 min of exposure to low-power commercial LED lights, respectively. Filtration studies using a dead-end stainless-steel cell reveal that both bacteria are eliminated (>99% after …

Study Of The Relationship Between Interoceptive Sensibility And Distress Tolerance In Community And College Samples, William W. Moore, Ashley Howell

Modern Psychological Studies

Interoceptive sensibility involves the detection, integration, and interpretation of internal bodily signals, primarily from cardiovascular, pulmonary, or gastrointestinal systems. Dysfunction in interoceptive processing has been increasingly recognized as a clinically relevant factor in numerous neurological, psychiatric, and behavioral disorders. This study aimed to elucidate the relationship between interoceptive sensibility and distress tolerance, hypothesizing that individuals with heightened interoceptive sensibility may report poorer distress tolerance. This relationship could help identify patterns between interoceptive sensibility and tendencies toward maladaptive coping strategies or emotional dysregulation. To explore these relationships, two studies were conducted utilizing self-report measures of interoceptive sensibility and distress tolerance in …

Born To Be Kind: The Evolutionary Significance Of Prosocial Behaviors In Humans, Yijia Xu

Modern Psychological Studies

Humans prefer kind romantic partners. Worldwide surveys have identified kindness as the defining feature of an ideal long-term partner, often explained as a signal for evolutionary fitness, demonstrating the ability to provide resources and ensure survival. Instead, we propose an alternative explanation: human preference for prosociality stems from an innate desire to be treated well. To test this, we asked participants whether they preferred kindness directed towards them or kindness exhibited more generally. Results indicate that participants were significantly more attracted to people who were kind to them personally than to people who were broadly kind to others. Interestingly, participants …

Leveraging Deep Learning For The Automated Detection Of Hemophilic Arthropathy In Knee Radiographs, En-Wei Lin, Edzer Wu, Ming-Ching Shen, Shao-Li Han

Rehabilitation Practice and Science

Background/Purpose:

Hemophilic arthropathy (HA) is a progressive joint disease caused by recurrent hemarthroses in patients with hemophilia. Early radiographic identification of HA is critical for timely intervention and long-term joint preservation. This study aimed to develop and evaluate deep learning models for automated detection of HA in knee radiographs.

Methods:

A retrospective dataset of 849 knee radiographs (AP and lateral views) was used to train three convolutional neural networks: ResNet-34, DenseNet-121, and EfficientNet-B0. Model performance was assessed using standard classification metrics, including accuracy, precision, recall, F1-score, and AUC. Interpretability was evaluated using Grad-CAM heatmaps.

Results:

DenseNet-121 achieved balanced performance on …

Diabetes Potentiates The Emergence And Expansion Of Antibiotic Resistance, John C. Shook, Benjamin P. Darwitz, Kaleb J. Tyson, Amanda Z. Velez, Sophia K. Bridwell, Joshua B. Parsons, Sarah E. Rowe, Christopher Marshall, Brian P. Conlon, Lance R. Thurlow

Biological Sciences Faculty Research and Publications

Individuals with diabetes mellitus frequently develop severe skin and soft tissue infections (SSTIs) that are recalcitrant to antibiotic treatment. We examined how diabetes affects the emergence of antibiotic resistance in a Staphylococcus aureus SSTI. We determined that S. aureus evolves antibiotic resistance rapidly in diabetic mice, while resistance did not occur in nondiabetic mice over the course of infection. Diabetes-associated immune cell dysfunction plays a minor role in the emergence of resistance, while hyperglycemia plays a dominant role facilitating the expansion and takeover of resistant mutants in diabetic infections. Furthermore, vancomycin intermediate resistant isolates display a pronounced fitness defect in …

Giant Cane (Arundinaria Gigantea) And The Swainson’S Warbler (Limnothlypis Swainsonii): Implementing Innovative Monitoring Techniques For Species Of Conservation Concern, Gabriela Carroll

Graduate Theses/Dissertations

In the U.S., following colonization and agricultural expansion, giant cane (Arundinaria gigantea) has been reduced to approximately 2% of its original extent, potentially causing shifts in the abundance and distribution of over 70 associated animal species. Among those species is the Swainson’s Warbler (Limnothlypis swainsonii), which has been known to occupy canebrakes during the breeding season. Though there is little known about the historical range of the Swainson’s Warbler and its current population status, some have hypothesized that its population may have declined due to the reduction of its suitable breeding habitat. As this species is …

The Long-Term Psychological Effects Of Violent Crime On Victims: A Review Of Evidence And Supportive Interventions, Jayden E. Oleksyn-Lamb

Graduate Theses/Dissertations

This study utilizes anonymous survey data collected from 26 mental health professionals in various regions of Canada to understand which psychological disturbances are the most prevalent among victims of violent crime as well as which psychological interventions are most beneficial for treating violent crime victims. The data revealed that Post-Traumatic Stress Disorder (PTSD), Major Depressive Disorder (MDD), Generalized Anxiety Disorder (GAD), anger, guilt, self-blame, and shame are each highly prevalent. Findings also revealed that certain therapeutic methods including Eye Movement Desensitization Reprocessing (EMDR), Supportive Counselling (SC), Progressive Relaxation (PR), Cognitive Reprocessing Therapy (CPT) and Cognitive Restructuring (CR) are associated with …

Tailwater Trout Population Dynamics, Connor Dylan Cunningham

Graduate Theses/Dissertations

Dams have been constructed throughout the world and provide several resources for humans (e.g., flood control, hydropower). These structures heavily alter the upstream and downstream environment. One consequence of these alterations is the creation of “tailwaters”, which can create cold-water habitat via hypolimnetic releases. In the southeast United States these tailwater environments are often managed for the recreational use of nonnative salmonids. The unnatural flow regimes of tailwaters affect the biotic and abiotic conditions and ultimately the dynamic rate functions (i.e., recruitment, growth, mortality) of the fish inhabiting them. As such, the goal of this research is to provide fisheries …

The Effect Of Hypochlorous Acid On Cultured Osteoblast And Escherichia Coli, Cristina Flaquer Fusté

Graduate Theses/Dissertations

Hypochlorous acid (HOCl), a naturally occurring compound in neutrophils, has been used as an antimicrobial in wound treatment. Osteoblasts, bone-forming cells, within the bone tissue play a large role in bone fracture healing. Orthopedic infections are challenging to treat and highly morbid, including surgical site infection of arthroplasty procedures or infection in the setting of compound fractures. HOCl is being considered as a surgical antiseptic to decrease infections in orthopedic surgical procedures. This study therefore seeks to determine the effects of HOCl on osteoblasts and E. coli. Cultured osteoblasts were incubated with varying concentrations of HOCl to examine osteoblast …

Characterization Of The Overexpression Of Reca Homologs Rad51 And Dmc1 In Tetrahymena Thermophila, Jianna M. Cox

Graduate Theses/Dissertations

RecA homologs, Dmc1 and Rad51, work to repair DNA double-strand breaks (DSBs) within the cell through the recombination of homologous sections of DNA. Dmc1 works to repair programmed DSBs through meiotic recombination, while Rad51 functions to repair both meiotic and non-meiotic DSBs, the latter repaired through the process of homologous recombination repair (HHR). Chemotherapeutics, exogenous agents, work to form DSBs in cancer cells, attempting to inhibit the cell’s growth. A hyper recombinant phenotype is often seen in cancer cells due to the overexpression of RAD51, leading to drug resistance, the persistence of cancers, and an overall poor patient outcome. …

Activity, Movement, And Reproductive Patterns Of The Northern Giant Musk Turtle (Staurotypus Triporcatus) In Belize, Larissa Sage Saarel

Graduate Theses/Dissertations

The Northern Giant Musk Turtle (Staurotypus triporcatus), a near-threatened species native to southern Mexico, Guatemala, Belize, and western Honduras, faces multiple environmental challenges, yet its activity patterns, movement patterns, and reproductive biology remain poorly understood. This thesis investigates both seasonal activity and reproductive cycles of female S. triporcatus in Belize. To assess diel and seasonal activity, I tracked 12 female turtles and collected data using activity loggers. I also collected movement data through radio-telemetry and acoustic tracking methods. Results indicated that the species is primarily nocturnal, with increased activity during the wet season, particularly during flooding events. Turtles …

Cyber Warfare And The Future Of Conflict, Victor A. Mercado

Graduate Theses/Dissertations

This thesis examines whether cyber warfare now poses a more immediate threat to U.S. national security than weapons of mass destruction (WMD). Cyber operations have become a dominant instrument of contemporary conflict, with malicious actors operating in a persistent “grey zone” that blurs traditional boundaries between war and peace. These operations target military and civilian entities, both directly and often as collateral damage due to the uncontrollable nature of cyber threats. WMDs, despite their catastrophic destructive potential, remain largely constrained by established deterrence frameworks.

The evolving nature of cyber warfare warrants comparison to WMD effects and impact, as cyber capabilities …

Responses Of Native And Invasive Aquatic Vegetation To Changes In Water Depth In An Ozark Spring-Fed Pond, Daphne Miles

Graduate Theses/Dissertations

Spring-fed aquatic ecosystems in the Ozark Highlands are characterized by stable water depths, clear water, and diverse aquatic vegetation (filamentous algae, submerged, floating, and emergent plants). Sudden changes in water depth can impact aquatic vegetation dynamics but the impacts of prolonged changes are poorly understood. I investigated seasonal and annual changes to aquatic vegetation abundance in a spring impoundment during and after a two-year period of low water depth that was caused by a broken outlet below an artificial dam. Water depth was significantly lower in 2020 and 2021 (low water years), compared to 2022 and 2023 (high water years). …

A Serum Biomarker Panel And Miniarray Detection System For Tracking Disease Activity And Flare Risk In Lupus Nephritis, Chenling Tang, Gongjun Tan, Aygun Teymur, Jiechang Guo, Arturo Haces-Garcia, Weihang Zhu, Richard Williams, Jing Ning, Ramesh Saxena, Tianfu Wu

Faculty, Staff and Student Publications

Introduction: Lupus nephritis (LN) leads to end stage renal disease (ESRD), and early diagnosis and disease monitoring of LN could significantly reduce the risk. however, there is not such a system clinically. In this study we aim to develop a biomarker-panel based point-of-care system for LN.

Methods: Immunoassay screening combined with genomic expression databases and machine learning techniques was used to identify a biomarker panel of LN. A quantitative biomarker-panel mini-array (BPMA) system was developed and the sensitivity, specificity, reproducibility, and stability of the were examined. The performance of BPMA in disease monitoring was validated with machine models using a …

Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Populations Of Central Illinois, Merylann Akinyi Ochieng

Masters Theses

The Asian tiger mosquito, Aedes albopictus, is a significant vector for diseases such as Dengue, Chikungunya, and Zika. Its increasing resistance to insecticides poses a critical challenge for vector control efforts. This study investigates knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) genes, specifically V1016G and F1534C, which are associated with pyrethroid resistance. Aedes albopictus populations from Central Illinois were analyzed using allele-specific PCR techniques to address three primary objectives: (1) determining whether V1016G and F1534C mutations are present in these populations, (2) determining which mutation is more widespread, and (3) investigating how resistance gene …

Time To Reassess: Campus Programming In The First Six Weeks And Its Impact On Belonging For First-Time Students, Joe Smith

Masters Theses

Research has shown that a student’s first six weeks of college have a strong impact on the rest of their college experience. Because of this, institutions concentrate programming efforts in the first six weeks of the academic year to increase the likelihood of positive outcomes for their first-time students. However, little research has been done on this topic in recent years. This quantitative study sought to examine first-time student event attendance in the first six weeks of the academic year and its impact on sense of belonging, which has been shown to be an important factor of the college experience. …