Digital Commons Network^™

Elevated Co2 Increases The Canopy Temperature Of Mature Quercus Robur (Pedunculate Oak), William Hagan Brown, Emanuel Gloor, Ralph Fyfe, A. Rob Mackenzie, Nicholas J. Harper, Peter Ganderton, Kris Hart, Giulio Curioni, Susan Quick, Scott J. Davidson, Emily Yetton, Jen L. Diehl, Sophie Fauset

School of Geography, Earth and Environmental Sciences

The canopy thermal response of natural forests to elevated CO₂ (eCO₂) is an understudied biophysical feedback in the global climate system. We investigated the effects of eCO₂ (150 μmol mol⁻¹ above ambient) on canopy temperature (T_can) dynamics of mature (> 175 years) Quercus robur (oak) at the Birmingham Institute for Forest Research Free Air CO₂ Enrichment (BIFoR-FACE) facility in Staffordshire, England, during the growing seasons of 2021, 2022 and 2023. We employed long-term, high-frequency thermal infrared (TIR) imaging to measure T_can. Our results show that daily maximum oak T_{can …}

Towards Developing Glut2 Specific Probes For Biochemical And Biomedical Applications, Abdelrahman Ismail

Dissertations, Master's Theses and Master's Reports

Facilitated Glucose transporters (GLUTs) are membrane proteins responsible for the uptake of simple sugars and other structurally similar metabolites in all mammalian cells. One of the hallmarks of cancer is dysfunctional sugar metabolism, known as the Warburg effect, and one of the manifestations of that effect is abnormal GLUT expression. There are 14 known GLUT proteins with different expression patterns in different tissues, and tumors tend to express certain GLUTs that are not present in surrounding healthy tissue. Therefore, selective targeting of GLUTs presents a potentially powerful tool for diagnostics and treatment of cancer and other diseases. This has been …

Near-Infrared Fluorescent Cyanine Probes For Monitoring Nad(P)H Dynamics In Living Cells And Drosophila Larvae, May Waters

Dissertations, Master's Theses and Master's Reports

Nicotinamide adenine dinucleotide (NAD+/NADH) plays a critical role in cellular metabolism, with abnormally elevated NADH levels linked to disrupted metabolic processes and cancer. In this study, we developed near-infrared fluorescent probes to selectively measure NADH concentrations. We present five cyanine-based probes, which were optimized by modifying the connection bridges to fine tune electronics. Two NAD(P)H-biosensing probes consisting of 1,3,3-trimethyl-3H-indolium and 3-quinolinium acceptors, linked by thiophene, A, and 3,4-ethylenedioxythiophene, B, bridges are detailed. They exhibit near-infrared fluorescence at 742.1 nm and 727.2 nm for probes A and B, respectively, and generate absorbance signals at 690.6 nm and 685.9 nm. Probe A …

Hybrid Mixtures Of Factor Analyzers For High Dimensional Data, Kazeem Abiodun Kareem

Dissertations, Master's Theses and Master's Reports

Factor analysis is a powerful tool for modeling latent structures in high-dimensional data, traditional approaches assume a single global structure, limiting their ability to capture heterogeneity. The Mixture of Factor Analyzers (MFA) extends classical factor analysis by modeling data as a mixture of Gaussian-distributed local subspaces, effectively uncovering cluster-specific latent structures. However, MFA relies on Gaussian mixtures, making it sensitive to outliers and ill-suited for heavy-tailed data. The Mixture of $t$-Factor Analyzers (M$t$FA) addresses these limitations by incorporating multivariate $t$-distributions, improving robustness. Despite their advantages, both MFA and M$t$FA face significant computational challenges in high-dimensional settings, particularly due to costly …

Near-Infrared Hemicyanine And Bodipy Sensors For Monitoring Mitochondrial Viscosity In Live Cancer Cells, Drosophila Melanogaster Larvae, And Adpkd Kidney Tissue, Joseph Peters

Dissertations, Master's Theses and Master's Reports

Two near-infrared fluorescent sensors, sensor A and sensor B, were synthesized and characterized. Sensor A was a hemicyanine derived structure, and sensor B was a BODIPY derived sensor. The sensors were tested to measure their ability to monitor viscosity, and sensor A was used to monitor viscosity in live cells. Both sensors were found to be effective in monitoring the viscosity level through optical measurements with glycerol. Sensor A was found to be successful in monitoring mitochondrial viscosity in HeLa MBA-MD-453 and MDA-MB-231 cell lines. It was also successful in monitoring viscosity in Drosophila melanogaster larvae as well as …

Initial Fungal Decomposer Community Determines Wood-Derived Carbon In Discrete Soil Carbon Pools In Aspen Clearcuts, Theresa K. Meyer

Dissertations, Master's Theses and Master's Reports

Soil carbon storage can be affected by forest management activities that alter the amount of biomass at a site, including slash retention and salvage harvesting. Initial fungal colonization of retained biomass may affect soil carbon storage due to differences in fungal decomposition strategies. To examine the effect of wood addition on wood-derived soil carbon storage and the interaction between initial fungal decomposer communities and wood application depth, δ¹³C modified wood inoculated with white- or brown-rot fungi was applied to soils across Michigan’s Upper Peninsula. Wood-derived carbon was assessed after 3.5 years in bulk soil samples and in three …

Sugar Maple (Acer Saccharum Marsh.) Tree-Ring Growth And Carbon Stable Isotope Responses To Nitrogen And Climate, Libbie Ring

Dissertations, Master's Theses and Master's Reports

This dendrochronological and stable carbon isotope study used tree rings from sugar maple (Acer Saccharum Marsh.) collected at long-term nitrogen (N) amended sites throughout Michigan to understand N amendment and deposition, climate, and growth relationships. Climate responses varied by site, however, we found importance in grouping sites by “productivity” which is related to inherent N and soil nutrients. Our results did not detect an overall effect of N amendments on growth during the treatment period. However, data shows N amendments benefiting larger diameter trees, while smaller diameter trees responded more positively without amendments, especially on more productive sites. Results …

Development Of Near-Infrared Probe For The Detection Of Nadh And Nad(P)H In Live Cells And D. Melanogaster Larvae, Sophia J. Jaeger

Dissertations, Master's Theses and Master's Reports

A near-infrared fluorescent probe, A, was designed by substituting the carbonyl group of the coumarin dye’s lactone with a 4-cyano-1-methylpyridinium methylene group and then attaching an electron-withdrawing NADH-sensing methylquinolinium acceptor via a vinyl bond linkage to the coumarin dye at the 4-position. The probe exhibits primary absorption maxima at 603, 428, and 361 nm, and fluoresces weakly at 703 nm. The addition of NAD(P)H results in a significant blue shift in the fluorescence peak from 703 to 670 nm, accompanied an increase in fluorescence intensity. This spectral shift is attributed to the transformation of A−π–A−π–D configuration to D−π–A−π–D pyridinium platform …

Barbie Vlogger As Teen Influencer For Little Girls, Brilynn A. Janckila

Dissertations, Master's Theses and Master's Reports

Mattel’s YouTube Barbie Vlogs series (2015-2022) targets 6–11-year-old girls with short narratives and prosocial messages addressing their prevalent interests and everyday experiences. While the vlog is clearly an attempt by Mattel to appeal to a market sector that includes both little girls and their Millennial parents, this analysis examines Barbie Vlogger as a social media influencer in order to explore how little girls learn to become older girls through the performance of teenage girlhood as enacted in the Barbie Vlogs. What is Barbie doing in the vlogs to show little girls who a teen girl is and what she …

Evaluating Challenges And Solutions For Buckthorn Classification In Shadowed Environments, Moses Tangwam

Dissertations, Master's Theses and Master's Reports

Invasive species, such as buckthorns, pose significant ecological threats by displacing native vegetation and reducing biodiversity. This study examines the impact of shadows on the classification accuracy of buckthorns using drone-based multispectral imagery collected in a forested area near Michigan Technological University. Shadows impacted approximately 70% of the imagery, notably distorting reflectance in key spectral bands such as near-infrared (NIR) and red edge. The study evaluated vegetation indices like NDVI and the performance of machine learning models, specifically the Random Forest classifier, under these shadowed conditions. Conventional shadow correction techniques, including histogram normalization, Shadow Index (SI), and Inverse Distance Weighting …

Microbial Inactivation And Spray Drying For The Recovery Of Single-Cell Protein From Chemically Deconstructed Polyethylene Terephthalate, Reeba Elizabeth Reji

Dissertations, Master's Theses and Master's Reports

Polyethylene terephthalate (PET) is one of the most widely used polymers in plastics packaging. However, the increasing demand for PET has created challenges in waste disposal. Previous research has shown that PET can be deconstructed using ammonium hydroxide (CDPET). Microorganisms capable of utilizing the product as a sole carbon source can be converted to single-cell protein (SCP). A custom microbial inactivation unit was developed to ensure the safety and stability of generated SCP. Our study evaluated the effect of exposure time (45–60 minutes) at 140 °C with the goal of maximizing microbial inactivation. Our findings suggest that microbial inactivation can …

Life Cycle Assessment And Life Cycle Cost Assessment Of A Floating Treatment Wetland In A Wastewater Lagoon Located In Copper Harbor, Michigan, Erin Browne

Dissertations, Master's Theses and Master's Reports

A full-scale floating treatment wetland was implemented in a wastewater treatment lagoon in Copper Harbor, Michigan. Copper Harbor is a small, rural community located in a cold climate. Many studies have focused on the performance of floating treatment wetlands in mesocosm studies in warmer climates, but there is not a lot of research conducted on the feasibility and performance of full-scale floating treatment wetlands in wastewater lagoons, specifically in cold climates. The 2024 treatment season was the first season with the floating treatment wetland implemented in the wastewater lagoon. A preliminary assessment of the cost and environmental impacts of a …

Community Diversity And Makeup Affect The Capacity For Bioconversion Of Chemically Deconstructed Pet Plastic Waste Into Biomass, Hunter Stoddard

Dissertations, Master's Theses and Master's Reports

Plastic waste management is an issue worldwide, particularly for locations with challenging logistics, such as remote locations, those impacted by natural disasters, and regions with armed conflict. These locations also struggle with food logistics and malnutrition. It is possible to tackle both issues by combining chemical deconstruction to depolymerize plastic waste with microbial conversion of the liquid product into biomass that can be used as an edible protein powder. The focus of this work is on the bioconversion of products from the chemical deconstruction of polyethylene terephthalate (PET) using ammonium hydroxide. Microbial communities have previously been shown to utilize the …

Growth, Survival, And Herbivore Browsing Preferences Of Seedlings Planted In Black Ash Stands Of Northern Michigan And Wisconsin, Seth W. Davenport

Dissertations, Master's Theses and Master's Reports

To mitigate the cascading ecosystem impacts of widespread black ash (Fraxinus nigra) mortality due to the invasive emerald ash borer (Agrilus planipennis), forested wetlands can be underplanted with seedlings of alternative species that later repopulate the vacated canopy. This study examined the survival and growth, factors influencing performance, and herbivore browsing preferences of seven non-ash species planted in northern Michigan and Wisconsin. The best performing species were river birch (Betula nigra), silver maple (Acer saccharinum), and swamp white oak (Quercus bicolor). The worst performing species was black spruce (Picea …

Direct Methane Capture Via Aerobic Methanotrophs, Waaseyaaban Landgren

Dissertations, Master's Theses and Master's Reports

Methane, a greenhouse gas with global warming potential 80 times greater than carbon dioxide, heavily contributes to global warming. Removing 1 Gt of atmospheric methane by 2050 would limit global temperature increase from reaching 1.5°C. Currently, biotrickling filter systems for removing atmospheric methane via methanotrophs exist, but not for very low methane concentrations (< 1 v%). Current research work at the University of Washington isolated and engineered a microbe strain which thrives at 500 ppmv methane, solving one obstacle in making this technology feasible. In this thesis, techno-economic analysis and environmental life cycle assessment conducted on this process have assessed its economic feasibility, greenhouse gas reduction potential, and possible areas of improvement. Study results show that at 500 ppmv methane, this process could remove atmospheric methane at a cost of $3,992-5,224 per tCH₄. The ideal case also produces annual net reduction in warming potential by 276-311 tCO₂e per process unit deployed.

Methods In Statistics, Machine Learning, And Deep Learning For Combining Multi-Omics Dataset, Md Mutasim Billah

Dissertations, Master's Theses and Master's Reports

Transcriptome-wide association studies (TWAS) have emerged as a powerful strategy to bridge genome-wide association studies (GWAS) with gene regulatory mechanisms by integrating genotypic data with gene expression data. While early TWAS methods typically rely on linear models and single-tissue expression references, recent advances underscore the need for flexible, multi-tissue approaches that can capture heterogeneous regulatory architectures and tissue-specific expression patterns. This dissertation introduces a three‑part research project that advances multi‑tissue transcriptome‑wide association studies (TWAS) along complementary axes of methodology, statistical power, and modelling flexibility.

In chapter One, TWAS‑CTL introduces a two‑stage cross‑tissue learner that trains any user‑chosen single‑tissue imputers (STLs) …

Design And Control Of Reversible Adhesion: Switchable Adhesive Molecules And Electrochemical Approaches, Kan Wang

Dissertations, Master's Theses and Master's Reports

Smart adhesives are materials that can reversibly gain or lose adhesion in response to external stimuli such as temperature, pH, electric fields, or light. Their switchable and temporary adhesion makes them highly promising for applications in wound healing, manufacturing, and robotic locomotion. Among them, catechol-based polymers have been widely studied for pH- and electro-responsive adhesion, due to their reversible non-covalent crosslinking. However, their performance is limited by irreversible covalent crosslinking reactions and the need for conductive surfaces in electrochemical systems. While boronic acid has been used to protect catechol, its reversibility remains limited. Therefore, new adhesive molecules and system designs …

Identifying Patterns In Avian Plasma Metabolites For Use In Comparing Between-Site Differences In Habitat Quality, Katherine A. Brouwer

Dissertations, Master's Theses and Master's Reports

Habitat loss and degradation are especially important considerations for migratory songbirds, since they depend on a variety of distinct habitats within different life phases (such as breeding, wintering, and migration) that are widely dispersed across a spatial scale. Additionally, abundance of migratory songbirds has been in severe decline, making habitat maintenance and recovery of particular interest as a research avenue. Identifying high quality habitats across a bird’s annual range is therefore of increasing importance and represents fundamental information for guiding management decision making. Free-floating metabolites found in blood are frequently used to determine short-term mass changes in birds and have …

Cyanine-Based Dual-Emission Sensor For Ratiometric Nad(P)H Imaging In Cells, Renal Tissues, And Entire Organisms, Peter Agyemang

Dissertations, Master's Theses and Master's Reports

Monitoring the dynamic fluctuations of NADH and NADPH, key coenzymes in Intracellular redox homeostasis and bioenergetics, are essential for understanding both normal physiology and disease pathology. However, precise, real-time imaging of NAD(P)H levels in complex biological systems remains a major challenge due to interference from endogenous fluorescence and the limitations of intensity-based sensors. We present a newly engineered dual-emission fluorescent sensor, sensor A, which overcomes these obstacles by offering ratiometric detection with high specificity and sensitivity. The sensor features a 3-quinolinium moiety conjugated to a cyanine dye scaffold via an amine linker, enabling selective reactivity toward NAD(P)H. Upon reduction, …

Design And Development Of Fluorescent Probes For Sensing And Bioimaging Of Nad(P)H, Intracellular Viscosity, And So2, Dilka Liyana Arachchige

Dissertations, Master's Theses and Master's Reports

Understanding the dynamic biochemical and biophysical changes within living systems is vital for advancing disease diagnosis and therapeutic development. This work presents the design and development of innovative fluorescent probes, based on cyanine and coumarin scaffolds, for the highly sensitive and selective detection of key metabolic and biophysical markers, NAD(P)H, mitochondrial viscosity, and sulfur dioxide (SO₂) in live cells and fruit fly larvae. A series of cyanine-based probes were engineered to undergo strong fluorescence enhancement upon reduction by NAD(P)H, enabling real-time visualization of metabolic fluctuations in cancer cells and Drosophila larvae. These probes exhibit excellent sensitivity, mitochondrial targeting capability, and …

Prediction Of Compressive Strength Of Fly Ash-Recycled Mortar Based On Grey Wolf Optimizer–Backpropagation Neural Network, Jing Jing Shao, Lin Bin Li, Guang Ji Yin, Xiao Dong Wen, Yu Xiao Zou, Xiao Bao Zuo, Xiao Jian Gao, Shan Shan Cheng

School of Engineering, Computing and Mathematics

The evaluation of the mechanical performance of fly ash-recycled mortar (FARM) is a necessary condition to ensure the efficient utilization of recycled fine aggregates. This article describes the design of nine mix proportions of FARMs with a low water/cement ratio and screens six mix proportions with reasonable flowability. The compressive strengths of FARMs were tested, and the influence of the water/cement ratio (w/c) and age on the compressive strength was analyzed. Meanwhile, a backpropagation neural network (BPNN) model optimized by the grey wolf optimizer (GWO), namely the GWO-BPNN model, was established to predict the compressive strength of FARM. The input …

Effects Of Invigorating And Relaxing Music On Heart Rate, Blood Pressure, And Heart Rate Variability, Matthew Joshua Faur

Honors Undergraduate Theses

Heart-rate variability (HRV) has been documented to correlate with several health measures, including autonomic nervous system longevity and athletic performance (Zulfiqar, 2010). While increasing age does not affect heart rate, there is a steady decline in HRV, which is a biological marker of aging and the decline of autonomic nervous system function. Furthermore, men who exercise regularly tend to have increased HRV, suggesting a mitigation of the typical age-dependent loss of HRV (Kristal-Boneh et al., 1995). Previous studies have suggested that music may have a physiological effect (e.g., fluctuating vitals, emotional changes) on cardiovascular parameters. However, little is known about …

Comparative Semiotic Analysis Of Non-Verbal Communication: Apis Mellifera Waggle Dance And Human Kinesic Signaling Systems, Nishtha Tikalal

Honors Undergraduate Theses

There are many parallels between two seemingly disparate forms of non-verbal communication: the waggle dance of Apis mellifera (honeybees) and human kinesic signaling systems. Through a comparative semiotic analysis, this research aims to conduct a comparative semiotic analysis, investigating structural and functional similarities between these communication methods, challenging anthropocentric assumptions about meaning making in the natural world. This research employs a meta-synthesis approach, drawn from a methodological literature review to examine historical representations of bee-human relationships, semiotics of non-verbal communication, and the specific mechanisms of the waggle dance and human kinesics. This methodology involves systematic categorization and analysis of extracted …

Respiratory Cycle Influence On Lumbosacral Muscle Function: A Tensiomyographic Analysis, Anthony Trombetta

Honors Undergraduate Theses

Introduction:

Tensiomyography (TMG) has significant potential in examining muscle functionality while evading the limitations found in other methods muscle analysis. Specifically, the erector spinae and other lumbosacral muscles can be analyzed with little challenge. Such analyses include data on muscle displacement (Dm), delay time (Td), contraction time (Tc), half-relaxation time (Tr), and sustain time (Ts). Of these parameters, the most evaluated and with the highest level of reliability are Dm, Td, and Tc. However, stiffness of the lumbar spine and lumbosacral muscles fluctuates throughout the respiratory cycle. These fluctuations may pose an issue when examining the functionality of muscles. This …

Impact Of Tgf-Β And Twist1 On Motility And Proliferation In Estradiol-Treated Mcf-7 Tamr Cells In-Vitro, Thomas L. Scott Iii

Honors Undergraduate Theses

Breast cancer is a highly malignant cancer and is known to be grouped into three main types: adenocarcinoma, invasive ductal carcinoma (IDC) and ductal carcinoma. Breast cancer commonly arises as adenocarcinoma, located in the milk ducts, and becomes IDC when it spreads into the surrounding tissue from the influence of cytokines and hormones. This study outlines the rate of proliferation and motility of a type of adenocarcinoma, MCF-7-TamR, to become invasive ductal carcinoma when Twist1 is downregulated and exposed to TGF- and estradiol (E2) in-vitro. We hypothesize that the downregulation of Twist1 will reduce cell adhesion and cytoskeleton proteins …

Tools And Trends: An Analysis Of Maya Dental Modification, Channing M. Cook

Honors Undergraduate Theses

The ancient Maya practiced dental modification, intentionally filing, incising, and inlaying stones into the front teeth, potentially to display identity and status. The literature surrounding Maya dental modification tends to overlook mechanical aspects of the process, and many studies approach dental modification through limiting or destructive means. Optical microscopy presents an adaptable, cost-effective option for closer inquiry. Preliminary research suggests the process of dental modification leaves distinct patterns of tool use, depending on style, that can be identified through optical microscopy. The sample selected includes 25 incisors and canines from 14 Classic period Maya individuals from Altun Ha, representing 12 …

Lost Innocence, Mateo Valdivieso

Honors Undergraduate Theses

Human trafficking is defined as involving “force, fraud, or coercion to obtain some type of labor or commercial sex act” (U.S. Department of Homeland Security). It disproportionately affects vulnerable populations, particularly children within the U.S. welfare system. This research aims to analyze the characteristics of this system that produce vulnerabilities in welfare children that heighten their risk of being trafficked. The study focuses on socio-economic, psychological, and environmental vulnerabilities, examining how these factors, combined with increased internet accessibility, exacerbate the risks of trafficking. Using a qualitative approach, the research uses secondary data from peer-reviewed journal articles, government reports, and reputable …

Measuring Achilles Tendon Stiffness And Kinetics In-Vivo, Chloe L. Pinkston

Honors Undergraduate Theses

This study aims to measure the gastrocnemii Achilles subtendon passive stiffness and energy storage during walking. Stiffness was first determined using a noninvasive method that combines ultrasound, motion capture, musculoskeletal modeling, and a force transducer. Eight healthy participants were tested using this method to obtain the passive force-displacement relationship of the Achilles tendon at two different knee angles to calculate stiffness of the gastrocnemii subtendon. A gait analysis session was then performed using ultrasound, motion capture, and an instrumented treadmill at self-selected slow, normal, and fast walking speeds, using stiffness to calculate the subtendon’s force and energy storage. Gastrocnemii Achilles …

Comparative Efficacy Of Hallucinogens In Treating Mood Disorders Through A Meta-Analysis Of Symptom Reduction, Dosage, And Duration, John Marco D.F. Muniz

Honors Undergraduate Theses

Background: Hallucinogens including psilocybin, lysergic acid diethylamide (LSD), ketamine, N,N-dimethyltryptamine (DMT) (as ayahuasca), have re-emerged as potential rapid-acting treatments for mood disorders. We conducted a meta-analysis of placebo-controlled trials evaluating their efficacy in depression and anxiety disorders. Methods: A systematic review identified 12 trials (Total ≈ 670) meeting inclusion criteria (randomized, placebo-controlled). Data on Cohen’s d and Hedges’ g effect sizes for depression- and anxiety-related outcomes were extracted. We computed pooled effect sizes (weighted by sample size and inverse variance), performed subgroup analyses by drug, diagnosis, follow-up duration, and outcome measure type, and assessed heterogeneity (I², Q …

Effect Of Metal Ions On The Reactive Oxygen Species Scavenging Capabilities Of Cerium Oxide For Biological Applications, Agastya Mittal

Honors Undergraduate Theses

Ceria nanoparticles' capabilities to defend cells against oxidative stress through superoxide dismutase activity are well-documented. Ceria nanoparticles demonstrate a ratio between 3⁺/4⁺ oxidative states on the surface of the particles to exhibit oxygen vacancies, a feature that allows these particles to scavenge Reactive Oxygen Species (ROS) in times of Oxidative Stress. Oxidative stress arises from many wounds in the body, such as wounds in the skin, and can have significant impacts on the health of an individual. Even when receiving treatment for the skin wound, ROS can circulate from around the wound, be generated from an imbalance …