Digital Commons Network^™

Gastrocnemius Myofiber Type And Mitochondrial Alterations Associated With Peripheral Artery Disease Severity, Kate Kosmac, Rena Dana Wang, Jada Stewart, Parminder Kaur, Ahmed Ismaeel, Haseeb Ahsan, Lisa Hartnell, Esther E. Dupont-Versteegden, Mary M. Mcdermott, Robert L. Sufit, Luigi Ferrucci, Charlotte A. Peterson

Physiology Faculty Publications

The extent of walking impairment varies among individuals with peripheral artery disease (PAD), which may reflect differences in the adaptability of lower extremity muscles to ischemia-reperfusion injury characteristic of the disease. Analyses of gastrocnemius muscle biopsies from 113 individuals with PAD [mean ankle-brachial index (ABI) = 0.65 ± 0.13, 38 (33.6%) women, 76 (67.2%) Black] showed a wide range of myofiber type distributions (9.6%-82.6% type 1 myofibers). The abundance of oxidative type 1 myofibers negatively correlated with ABI (r = −0.22, P = 0.02), a measure of PAD severity. The abundance of type 1 myofibers also negatively correlated to 2a/x …

Inter-Rater Reliability Of Muscle Ultrasonography Performed By Multidisciplinary Novice Sonographers In The Evaluation Of Critically Ill Patients With Acute Kidney Injury Requiring Continuous Kidney Replacement Therapy, Felipe González-Seguel, Vinh Q. Tran, Chaitanya Anil Pal, Zan T. Shareef, Hayley P. Israel, Arimitsu Horikawa-Strakovsky, Yuan Wen, Benjamin R. Griffin, Javier A. Neyra, J. Pedro Teixeira, Kirby P. Mayer

Physiology Faculty Publications

Early diagnosis of muscle wasting in critically ill patients with acute kidney injury requiring continuous kidney replacement therapy (AKI-CKRT) may improve outcomes via timely rehabilitation and nutrition. Muscle ultrasound (MUS) has recently gained traction for assessing muscle atrophy in the intensive care unit (ICU) but requires training to achieve reproducibility. We evaluated the inter-rater reliability of MUS in patients with AKI-CKRT performed by multidisciplinary raters including nephrologists. Two blinded independent raters used portable ultrasound to acquire images of the rectus femoris (RF). All raters were clinicians routinely caring for patients with CKRT in the ICU and were initially novices in …

Neuro293, Joshua T. Moses, Fahad B. Shah, Nicholas M. Mcvay, Dylan E. Capes, Christopher C. Bosse-Joseph, Jocelyn Salazar, Victoria K. Slone, John E. Eberth, Jonathan Satin, Andrew N. Stewart

Physiology Faculty Publications

Efficient interrogation of neurobiology remains bottlenecked by obtaining mature neurons. Immortalized cell lines still require lengthy differentiation periods to obtain neuron-like cells, which may not efficiently differentiate and are challenging to transfect with plasmids relative to other cell lines such as HEK-293's. To overcome challenges with limited access to cells that express mature neuronal proteins, we knocked out the RE1-silencing transcription factor (REST) from HEK-293's to create a novel neuron-like cell, which we name Neuro293. RNA-sequencing and bioinformatics analyses revealed a significant upregulation of genes associated with neurobiology and membrane excitability including pre-/post-synaptic proteins, voltage gated ion channels, neuron-cytoskeleton, as …

S6k1 Is A Targetable Vulnerability In Tumors Exhibiting Plasticity And Therapy Resistance, Saptadwipa Ganguly, Ravshan Burikhanov, Vitaliy M. Sviripa, Sally Ellingson, Jieyun Jiang, Christian M. Gosser, David Orren, Eva M. Goellner, Gautham G. Shenoy, Mahadev Rao, John D’Orazio, Christine F. Brainson, Chang Guo Zhan, Peter H. Spielmann, David S. Watt, Vivek M. Rangnekar

Physiology Faculty Publications

Background: Most tumors initially respond to treatment, yet refractory clones subsequently develop owing to resistance mechanisms associated with cancer cell plasticity and heterogeneity. Methods: We used a chemical biology approach to identify protein targets in cancer cells exhibiting diverse driver mutations and representing models of tumor lineage plasticity and therapy resistance. An unbiased screen of a drug library was performed against cancer cells followed by synthesis of chemical analogs of the most effective drug. The cancer subtype target range of the leading drug was determined by PRISM analysis of over 900 cancer cell lines at the Broad Institute, MA. RNA-sequencing …

Skeletal Muscle Stem Cell Mitochondria Are Transferred To Muscle Fibers In Response To A Hypertrophic Stimulus, Jensen Goh, Julia G. Williams, Sarah E. Ogle, Jai K. Joshi, Logan N. Scott, Benjamin Burke, Alex R. Keeble, Nicholas T. Thomas, Christopher S. Fry, Ahmed Ismaeel, John J. Mccarthy

Physiology Faculty Publications

The fusion of skeletal muscle stem cell (MuSC) to myofibers during hypertrophy has exclusively focused on the transfer of the MuSC nucleus, leaving the fate of other MuSC organelles, such as mitochondria, largely unexplored. The objective of this study was to determine whether MuSCs transfer their mitochondria upon myofiber fusion in response to a hypertrophic stimulus. To achieve this goal, we specifically labeled MuSC mitochondria with Dendra2 fluorescence by crossing the MuSC-specific CreER (Pax7^CreER/CreER) mouse with the Rosa26-Dendra2 mouse to generate the Pax7-Dendra2 mouse. To induce the fusion of MuSC to myofibers, Pax7-Dendra2 mice underwent synergist ablation surgery …

Establishing Metric Method’S To Identify Variation In Human Biological Sex Using The Upper And Lower Limb Bones Of Non-Population Specific Samples., Roland P. Sanchez

Graduate Student Theses, Dissertations, & Professional Papers

Sex estimation of human skeletal remains is a facet of the biological profile. The pelvis has been suggested as the best source to accurately predicting the biological sex, but not always available after the recovery of human remains. At times, only long bones of human skeletal remains are recovered for identification. It has been shown that metric measurements using long can accurately predict biological sex. The most common methods use discriminant functions to predict the likelihood an individual falls within a certain population. These studies rely on population specific collections of skeletal remains for increased accuracy. It’s advised to avoid …

Seahorsedad: A Study Of Metaphors In Transmasculine Birther’S Instagram Posts, Alexa Anne Runnion

Graduate Student Theses, Dissertations, & Professional Papers

Dominant notions of pregnancy frame gestation as a solely female process. However, the experiences of transmasculine birthers challenge this assumption, demonstrating that pregnancy is not inherently tied to womanhood but is instead shaped by social and cultural narratives and representations. This paper explores the unique pregnancy and parental exercises of transmasculine birthers by examining public social media posts under the hashtag #seahorsedad on Instagram. Utilizing metaphorical criticism, I aim to uncover how transmasculine birthers, known within the community as “seahorse dads,” both uphold and resist traditional notions of gender, pregnancy, and parenting through a combination of textual and visual metaphor. …

Aspen Stands Enrich Bird Communities Following A Wildfire In A Montane, Mixed Conifer Forest, Cody Q. Lane

Graduate Student Theses, Dissertations, & Professional Papers

Fire is a major ecological force in most terrestrial systems, but the responses of animal communities to fire vary markedly across ecosystems due to variability in fire regimes and vegetation communities. Quaking aspen (Populus tremuloides) is unique within coniferous forests of western North America for its ability to regenerate following wildfire via vegetative resprouting. As managers employ techniques to restore fire regimes and conserve declining aspen forests, it becomes important to understand how aspen alters postfire communities. Our objectives were to (1) assess how aspen interacted with fire severity and years since fire to influence avian species and …

Spatial And Temporal Patterns Of Nutrient Limitation In A Western Montana River, Matthew T. Nichols

Graduate Student Theses, Dissertations, & Professional Papers

Enhanced loading of nitrogen (N) and phosphorus (P) is pervasive in rivers around the world. As a consequence of nutrient-rich conditions, many well-lit rivers experience severe algal blooms, which threaten water quality and aquatic habitats. Nutrient enrichment in the upper Clark Fork River, located in western Montana, has increased the productivity of benthic algae, Cladophora glomerata, which routinely forms extensive blooms during summer growing seasons (July – September). Addressing eutrophication in rivers begins with identifying the nutrients that contribute toward algal productivity. I deployed nutrient diffusing substrates (NDS), collected bi-weekly surface water samples, and conducted algal surveys at four …

Fuel Break Effectiveness – Determining Ignition Thresholds Based On Radiant And Convective Energy Transfer, Daniel Michael Jimenez

Graduate Student Theses, Dissertations, & Professional Papers

Extended fire seasons and increased fire severity in and around protected ecosystems and the wildland urban interface (WUI) will increasingly challenge fire suppression, impact communities and threaten property and resources. Addressing this growing threat through proactive management solutions that protect values at risk requires improved fire behavior prediction capabilities.

Here I explore the radiant and convective energy transfer mechanisms of fire ignition or extinction associated with fuel discontinuities. Through a series of wind tunnel experiments I identified energy and ignition thresholds unique to changing wind speed, fuel moistures and fire line characteristics, and developed a multi-variate linear regression model to …

Indigenous Placemaking, Colonial Placetaking, And Indigenous Data Sovereignty: Implications Of Environmental Data Collection On Tribal Lands, Paige Nizhonii Kya'iyo Johnson

Graduate Student Theses, Dissertations, & Professional Papers

This project investigates how Indigenous Data Sovereignty protocols apply to environmental data collected on Tribal lands by federal and state entities. The 2011 and 2014 flooding of the Missouri River and a need for further soil moisture data led the United States Army Corps of Engineers Omaha District to implement a Mesonet weather station monitoring network across the Upper Missouri River Basin (UMRB). Historically, the Army Corps of Engineers has not included Indigenous perspectives into their decision-making, and this has caused Tribes to be distrustful of partnerships with them. The UMRB monitoring network will span across the Upper Missouri River …

Hormonal Decay After Death: Evaluating Dhea-S As A Potential Molecular Marker For Postmortem Estimation, Marisa Lydia Novak

Graduate Student Theses, Dissertations, & Professional Papers

Post-mortem interval (PMI) estimation is a central component of forensic anthropological investigation, but traditional methods for estimating time since death lose accuracy after ~72 hours. Recent research indicates that some steroid hormones may undergo predictable degradation patterns after death, offering potential use as biochemical clocks for PMI estimation beyond this threshold. Dehydroepiandrosterone (DHEA), and its sulfate form (DHEA-S) are of particular interest due to their metabolic stability, systemic influence, and association with trauma. This study investigates whether DHEA-S hormonal degradation patterns correlate with time elapsed since death and body temperature, evaluating their potential as biochemical PMI markers. 160 mL of …

Structural And Dynamic Properties Of Gas-Phase Molecules In High-Order Harmonic Generation: Insights From Time-Dependent Density Functional Theory Calculations, Christopher Michael King

Graduate Student Theses, Dissertations, & Professional Papers

High-order harmonic generation, or HHG, is a highly nonlinear quantum optical process capable of generating X-ray/ultraviolet radiation. An atom or molecule is irradiated with a mid-IR laser at high intensity, ionizes, and then emits high-frequency light at integer multiples of the driving laser frequency when an ionized electron recollides with the molecular ion. A convenient tabletop source of coherent XUV light and a common seed for free-electron lasers, HHG can also serve as a self-probe of the source atom or molecule. In the latter case, the HHG spectrum can provide valuable insight into the structure and dynamics of the species …

Editorial: A Decade Of Apasti – Advancing Regional Prosperity Through Science, Technology And Innovation, Basilios Tsikouras

ASEAN Journal on Science and Technology for Development

A decade after its establishment, APASTI has emerged as a key regional mechanism for promoting science, technology and innovation as drivers of sustainable development, resilience, and shared prosperity in Southeast Asia. This editorial highlights the contributions of APASTI to policy alignment, knowledge exchange, research visibility, and collaborative network-building across ASEAN, while also acknowledging ongoing constraints related to governance, capacity, funding disparities, and uneven regional integration. It introduces a special issue of 14 papers published in AJSTD that collectively evaluate the implementation and impact of APASTI across diverse sectors, including geohazards, energy, materials, health, infrastructure, transport, and information systems. By positioning …

Managing Digital Transformation With Blockchain: Distributed Ledger Innovation Platforms, Nobuyuki Fukawa, Gregory J. Fisher

Business and Information Technology Faculty Research & Creative Works

Realizing its overarching potential in achieving digital transformation, leading organizations, such as Air New Zealand, have recently employed blockchain technology to manage 3D printing innovation. Despite the potential benefits, including the key role of blockchain as a supporting technology in Industry 5.0, these applications of blockchain technology in 3D printing are still at a nascent stage in practice and mostly limited to producer innovation. Additionally, 3D printing and blockchain technologies are often independently revolutionizing value capture and value creation, whereas the theoretical implications of their combined impacts on innovation management remain an underexplored domain. Limited digital transformation efforts have been …

The Antibiofilm Efficacy Of Copper And Zinc-Enhanced Borate Bioactive Glasses On Polymicrobial Biofilms, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Healthcare-acquired infections (HAIs) are a significant global challenge driven by biofilm-forming pathogens. Polymicrobial biofilms, involving interactions between multiple microbial species, exacerbate treatment difficulties due to their enhanced resistance to antimicrobial therapies. Borate bioactive glasses (BBGs) are an emerging class of biomaterials that have attracted significant interest in infection control. The incorporation of copper and zinc into the BBG matrix can effectively disrupt biofilm formation and bacterial colonization. This study investigates the antibiofilm efficacy of copper and zinc-doped BBGs against polymicrobial biofilms formed by S. epidermidis, E. coli, and P. aeruginosa. Using static and dynamic biofilm models, BBGs were applied through …

The Role Of Organizational Routines In Perpetuating Disparities Among African American Students In Secondary Advanced Academic Programs: A Mixed Methods Study, J. Nichelle Wimbush

Theses and Dissertations

The academic excellence gap, which refers to the disproportionalities of minority groups in advanced academic programs, presents challenges for educational leaders striving to provide access to equitable educational opportunities for all students. This study, guided by Charles Tilly’s opportunity hoarding, examines the systematic underrepresentation of African American students in advanced academic programs. Using a transformative mixed methods design, the researcher analyzes enrollment patterns, the intersectionality of race and gifted identification in K-5, and teacher and leader perceptions of how organizational routines influence student enrollment in advanced academic courses, grades 9-12, in a school district in West Central Georgia. Redacted student …

An Evaluation Of Frank Brown's Free Market, Leigh Ann Mccormick

Theses and Dissertations

Background: This study aimed to conduct an in-depth evaluation of Frank Brown Hall, Free-Market (Food Pantry), within the grant timeframe from January to April 2024. The central objective was to examine the distinct resource demands of students limited to selecting three items each day. By employing careful observation, effective communication, and active listening, we successfully obtained essential student feedback that enhanced our understanding of their needs and preferences, improving the support provided during that semester. Methods: Throughout the grant, data were systematically classified according to the chosen items, the students’ academic majors, and the cumulative number of visits to the …

Targeting Als: A Multi-Cellular Study On The Potential Therapeutic Role Of Azole-Based Compounds, Alisha R. Kennedy

Theses and Dissertations

Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig’s disease, is a progressive neurodegenerative disorder that leads to the degeneration of motor neurons, which control voluntary muscle movements such as eating, speaking, and breathing. As these neurons are damaged, voluntary movement ceases, leading to a fatal outcome. The survival time of ALS patients varies, ranging from a few months to up to 10 years. Chapter 1 of this thesis provides an overview of ALS, highlighting the role of transactive response DNA-binding protein 43 (TDP-43), a key feature in ALS and other neurodegenerative diseases. The aggregation of TDP-43, particularly due to …

Purification Of Recombinant His6-Gfp-Preptin Fusion Protiens, Apoorva Kollaram

Theses and Dissertations

Preptin, a 34-amino-acid peptide derived from proinsulin-like growth factor II, has emerged as a promising therapeutic candidate for both Type 2 diabetes mellitus and osteoporosis. Its dual functionality—stimulating insulin secretion without inducing hypoglycemia and promoting osteoblast proliferation—positions it as a unique alternative to traditional sulfonylureas and osteogenic agents. In this study, we developed a purification protocol for green fluorescent-preptin fusion proteins. These fusion proteins will be used to investigate the functional significance of residues Pro17, Val18, Gly19, Trp27, Lys28, and Gln29 in rat preptin, which shares high sequence similarity with the human form. Site-directed mutagenesis was performed using NEB Q5 …

Understanding Women’S Attitudes Toward Midwifery Practices For Maternal Care In Middle Georgia, Kristen M. Simpson

Theses and Dissertations

Compared to other high-income countries, women in the United States are more likely to die from problems related to pregnancy and childbirth. In Georgia, maternal mortality is particularly acute. Midwifery models of maternal care have been shown to produce positive pregnancy and childbirth outcomes for patients, but these services are underutilized in Georgia. This study sought to identify women’s attitudes toward midwifery, what contributed to those attitudes, and what influenced women in Middle Georgia to seek midwifery services using a qualitative, Grounded Theory approach. Three participants who self-identified as having used a midwife before were interviewed using a semi-structured protocol. …

Lights, Camera, Action: A Student's Perspective On Reimagining Film Production Opportunities, Ma'lon I. Lane

Theses and Dissertations

The pursuit of a crew job in the film industry is a multifaceted endeavor, offering various pathways that cater to diverse aspirations and circumstances. Central to this exploration is the question of whether attending a university and obtaining a degree in film is essential for success. I was determined to answer the following: What is the likelihood that a higher education in Film Production leads to more efficient job acquisition in the film industry? The project is considered narrative, originating from the personal quality of the research questions. I used my resources to gather qualitative data through interviews of film …

Why A Healthy Diet Is Important For Women's Health, Halley Doster

Theses and Dissertations

Nutrition plays a critical role in women’s reproductive health, yet many women face barriers to maintaining a balanced diet. This study aimed to examine the relationship between nutritional intake and reproductive health outcomes among women of reproductive age, as well as to assess their knowledge and challenges related to healthy eating. Using a cross-sectional survey design, data were collected from 50 women at a community health center and analyzed using SPSS to generate descriptive statistics and explore patterns through chi-square and regression analyses. Findings revealed that most participants had moderate to low fruit and vegetable intake, and fewer than half …

Attacking Diabetes One Day At A Time, Kyla Duncan

Theses and Dissertations

The "Attacking Diabetes One Day at a Time" initiative targets the growing concern of public health, type 2 diabetes, among under-reserved communities. This comprehensive care program emphasizes the importance of prevention through education, improving lifestyles, and access to care. Based on insights from the Self-Care Management Theory, this initiative seeks to provide individuals with sound, culturally tailored strategies that reduce disease risk and help them manage healthy lifestyles. Major components include educational workshops, and resource navigation. The success of this program, from community engagement through evaluation on a phased implementation plan, is guaranteed. The expected outcomes of this work will …

Online Parameter Adaptation Of Lqr Controllers Via Rls For Prosthetic Joint Control: Experimental Validation On A Quanser Qube-Servo 2 Platform, Cynthia Lopez-Jordan

Theses and Dissertations

This thesis develops and experimentally validates an online adaptive Linear Quadratic Regulator (LQR) control method for prosthetic joint systems using Recursive Least Squares (RLS)-based real-time parameter estimation on the Quanser QUBE-Servo 2 platform. Traditional LQR controllers assume a fxed system model, which limits adaptability and results in reduced tracking accuracy, poor robustness, and loss of optimal performance when applied to dynamically changing prosthetic joints, infuenced by load variations, user gait changes, and mechanical wear. Limited experimental validation exists for combining RLS with online LQR adaptation in prosthetic-like systems.

To address these limitations, an RLS-driven Adaptive LQR framework was implemented to …

An Analysis Of Coal Ash Pollution In The State Of Georgia, Mick W. Etchison

Theses and Dissertations

This social science paper will explore the factors contributing to the prevalence of coal ash in specific regions of Georgia and its impact on local ecosystems and human health. It provides an analysis of the current environmental and political issues surrounding the containment and spread of coal ash throughout the State. Coal ash is primarily found in the central, southwestern, and northeastern regions where coal-fired power plants and coal ash storage sites are located. The paper will then discuss the political, economic, and legal reasons behind the lack of effective regulation and mitigation of coal ash spread. Coal ash illustrates …

From Curiosity To Controversy: American Perception Of Japanese Immigrants, 1853-1924, Chloe Elizabeth Curbelo

Theses and Dissertations

In the mid-nineteenth century, as the United States reached the Pacific through westward expansion, Asia emerged as a new stage for commerce and power. Japan, long isolated under the Tokugawa shogunate, became an object of American fascination when forced into relations. While early depictions cast Japan as an exotic yet uncivilized nation in need of Western guidance, Japan’s rapid modernization and the growing number of Japanese immigrants on the West Coast transformed curiosity into anxiety. My paper traces this evolution of perception from 1850 to 1924, arguing that shifting views of Japan and its people reflected America’s ethnocentric notions of …

How Do School Counselors And School Social Workers Support High School Bullying Victims: A Qualitative Exploratory Case Study, Dalentina N. Robertson

Theses and Dissertations

Bullying and student aggression are increasing in public high schools. Bullying and cyberbullying are a safety concern, and victims of bullying have shown a decline academically and emotionally. Additionally, high schools need increased support with bullying behaviors. The purpose of this exploratory case study was to explore school counselors’ and school social workers’ insights and perspectives regarding the support they provided to bullying victims in two school districts in rural Georgia. Bronfenbrenner’s Ecological Systems Theory was the framework used to focus research on understanding how environmental systems influence an individual’s development. Semi-structured interviews were conducted from a purposeful sample of …

Nanotube Spectral Fingerprinting And Machine Learning For Optimized Bioimaging/Sensing And Disease Detection Applications In Als, Rodrigo Monroy Lopez

Open Access Master's Theses

Single-walled carbon nanotubes (SWCNTs) possess unique physicochemical and optical properties that make them ideal candidates for biomedical imaging, biosensing, and disease diagnostics. This thesis explores the potential of SWCNT-based spectral fingerprinting combined with ML (Machine Learning) algorithms to optimize bioimaging, disease detection and prediction, with a specific focus on differentiating between healthy and amyotrophic lateral sclerosis (ALS) lymphoblastic patient samples. By functionalizing SWCNTs with single-stranded DNA, we enhance their stability and target specificity, enabling their application in serum patient samples.

A comprehensive spectral analysis of DNA-SWCNTs was conducted using near-infrared fluorescence spectroscopy and other characterization techniques, including UV-Vis-absorption spectroscopy. The …

The P3 Event-Related Potential (Erp) In Visual And Auditory Tasks: Implications For Executive Functioning In Adhd Preadolescent Children, Alex Visal Seng

Open Access Master's Theses

Attention-Deficit/Hyperactivity Disorder (ADHD) is a widespread psychological disorder in children, poorly impacting executive functioning domains of inhibitory control and working memory. Event-related potentials (ERPs) provide neuroelectric insight into these cognitive processes. However, most ERP research focuses on one task type, limiting direct comparisons of cognitive demands across different task paradigms. This study examined P3 amplitude and latency in ADHD children using a visual NoGo task (response inhibition) and an auditory oddball task (working memory), and associations between the P3 ERP and behavioral measures of executive functions.

Cross-sectional data of symptomatic ADHD children (N=13; F=5; aged 9.15 ± 1.86 years) were …