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Machine Learning-Based Risk Stratification For Limb-Related Outcomes In Peripheral Artery Disease Patients Undergoing Percutaneous Transluminal Angioplasty, Yujia Zhang, Zhoudong Jing, Zhiqiang Liu, Angel Lai, Guangming Tan, Bryan P Yan

Journal of the Hong Kong College of Cardiology

Background: Patients with peripheral artery disease (PAD) remain at high risk of mortality and major adverse limb events (MALE) after endovascular revascularization, yet outcome prediction tools are limited. We apply machine learning (ML) to improve prognostic stratification in this population. Methods: PAD patients undergoing percutaneous transluminal angioplasty (PTA) between 2007 and 2024 were included. The primary endpoint was 1-year major adverse limb events (MALE) or all-cause death. Data were divided into training (70%) and testing (30%) sets. Six ML models were developed, and performance was evaluated using the area under the receiver operating characteristic curve (AUC). Feature importance and risk …

Examining Collaborative Healthcare For Substance Use Disorder: A Case Study Of Brigham And Women's Hospital Bridge Clinic, Megan Bidgood

Psychology Honors Papers

Individuals with substance use disorders (SUD) are clinically underserved due to increased health and psychiatric comorbidities, structural barriers to treatment, stigma, and insufficient resources. This suggests that there is a need for an accessible and culturally competent treatment model. Brigham and Women’s Hospital (BWH) Bridge Clinic is a multi-disciplinary, low threshold SUD treatment to promote collaborative healthcare and the transitioning of patients to longer-term care. The aim of the current study is to better understand personal accounts of patients and various clinical and non-clinical providers to optimize the BWH Bridge Clinic’s approach to treatment. Patients (N=5) and providers (N=6) participated …

Journal Of Earth And Life Science, Academic Year 2024-2025, Megan Boche, Nathan D. Pyburn, Ben Hoover, Jayden R. Alsleben, Hector Montoya, Jr, Reed Cumberland, Rose Kuhlmann, Ray Anderson, Ellie Isakson, Seth Sisneros-Martinez, Zanden Mellen, Quady Bernu, Cian Verbridge, Benjamin D. Strei, Madelaine Adamich, Arian Garcia, Tegwen Romportl, Sandra J. Marcu, Bemidji State University

Journal of Earth and Life Science

No abstract provided.

Size Structure Of Black Crappie And Bluegill In Northern, Eastern, Western, And Southern Minnesota, Ray Anderson

Journal of Earth and Life Science

Fish size structure is an important factor in a fishery population. Panfish such as black crappie Pomoxis nigromaculatus and bluegill Lepomis macrochirus play a major role in the health of fisheries. Five lakes from southern, northern, eastern, and western Minnesota were selected for analysis. Data were collected using standard sampling methods, including gill nets, electrofishing, and fyke nets. Length-frequency distributions were created to assess size structure in each region. Relative abundance was measured using catch per unit effort. Data analysis included a one-way ANOVA to compare Proportional Size Distribution (PSD) values and the distribution of fish sizes among regions. Chi-square …

Comparison Of Hoop Nets To Trap Nets In Sampling Panfish, Jayden R, Alsleben

Journal of Earth and Life Science

Panfish including bluegill Lepomis macrochirus and black crappie Pomoxis nigromaculatus are gamefish species of significance found in Minnesota. Hoop nets and trap nets are two types of gear that can be used to sample these fish. Fisheries managers and anglers alike are interested in the best tactics to capture panfish. The behavior of fish, including bluegill and black crappie, change throughout the sampling season and make it difficult to assume accurate representations. Trap nets have historically been adequate in sampling near shore populations, but questions surrounding the gear have allowed other options to be pursued. The objective of this study …

The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia

Journal of Earth and Life Science

Microplastics, plastic particles smaller than 5 mm, are an increasing environmental threat, particularly due to their widespread distribution and toxicity. While much research has focused on larger freshwater and marine ecosystems, the presence and impacts of microplastics in smaller inland lakes remain less understood. This study investigates the relationship between plant density and microplastic concentrations in Lake Bemidji, a smaller freshwater lake. A total of 30 water samples were collected across varying vegetation conditions, ranging from open water (0%) to 90% plant coverage. Microplastics were identified and categorized into six morphological types: fibers, fragments, films, spheres/beads, and foam. The samples …

A Sample Of Fish Communities In Beaver Ponds Of Northern Minnesota, Reed Cumberland, Rose Kuhlmann

Journal of Earth and Life Science

The North American beaver Castor canadensis is an ecosystem engineer and a keystone species to the environment. Beaver inhabit ponds and construct dams to build ponds that provide habitat for certain fish. Little information is provided on the species of fish that live in beaver ponds. The objective of this study is to sample the fish communities in beaver ponds while comparing the effectiveness of two styles of minnow traps. Twelve beaver ponds were selected in the Bemidji area to be sampled. The quantity and species caught in each style of trap were recorded to compare effectiveness of each trap …

Effects Of Round-Up On The Environment, Sandra J. Marcu

Journal of Earth and Life Science

Many people around the world have used and still currently use Roundup but are unaware of the effects it has on the environment. Roundup is a spray on application weedkiller that is widely used around the world today both residentially and commercially. It enables its user to grow a garden or a field of crops with a no-tilling approach to eliminate weeds. It is a well-known and popular choice for killing weeds that has been around since the mid 1970’s (Oca, 2017). John Franz, a Monsanto scientist discovered that glyphosate (main ingredient in Roundup) was an herbicide or weedkiller, and …

The Effectiveness Of The Minnesota Buffer Law And Its Impacts On People, Ellen P. Isakson

Journal of Earth and Life Science

In January 2015, the then-governor of Minnesota Mark Dayton announced new legislation that would require 50-foot vegetation buffers along all waterways in Minnesota; this legislation would come to be known as the Minnesota buffer law, exact requirements being “perennial vegetative buffers of up to 50 feet along lakes, rivers, and streams and buffers of 16.5 feet along ditches” (Albert, 2017; MN Board of Water and Soil Resources [MN BWSR] 4 ). A buffer in the context of vegetation and natural resource management is a strip of vegetation around a body of water “designed to intercept stormwater runoff and minimize soil …

Does Depth Affect Zebra Mussel Length In Scalp Lake?, Zanden Mellen

Journal of Earth and Life Science

Since its first sighting in 1996, zebra mussels Dreissena polymorpha have been confirmed in hundreds of Minnesota lakes. Not only were they found in lakes, but they are also found in rivers too. With lack of effective treatments to impede their spread, zebra mussels continue to colonize new areas causing ecological as well as economic harm. The objective of this study was to see if there was a relationship between a zebra mussel length and the depth, they are found using shell length (mm) and depth (cm). The comparison of zebra mussel length and depth was done by performing a …

Age Class Is Not Related To Size Or Composition Of Home Ranges Among Suburban White-Tailed Deer During Parturition, Nathan D. Pyburn

Journal of Earth and Life Science

How white-tailed deer use the landscape in an urban environment can help us understand the effects of urbanization on deer populations. White-tailed deer form observable matriarchal hierarchies in studied populations. This study examines the matriarchal structure, home range size, and home range composition of 25 collared white-tailed deer within the city limits of Bemidji, MN. The results found no significant difference between age class and home range size or composition. However, movement metrics from yearling and mature age classes were more similar than the juvenile age class females. I propose this is because social acceptance between mature females was higher …

The Growth Of Yellow Perch In Relation To Environmental Factors, Hector Montoya Jr.

Journal of Earth and Life Science

Yellow perch Perca flavescens is one of the major fishes in northern Minnesota. These fish are important to the ecosystems they live in but, these   ecosystems are subject to change at anytime if the factors present themselves. Research was done in the past that   studied water temperature, air temperature, climate events, and human activity showing their effect on fish growth. This study looks at the same four factors that influence the ecosystem in Lake Bemiji during the month of September in the years of 2017-2024. Each factor is compared to the average total lengths of yellow perch to test for …

Relationship Of Utricle And Trap Door Size On Common Bladderworts Utricularia Macrorhiza, Tegwen Romportl

Journal of Earth and Life Science

The common bladderwort Utricularia macrorhiza is a carnivorous aquatic plant that captures organisms with its bladders. This study was aimed at setting the stage for future research which might determine if U. macrorhiza could be a biological method of control for invasive species. The objective of this study was to determine the relationship between utricle and trap door sizes and to gather data on the size ranges of these two morphological features. A regression analysis was used to obtain results which showed there was enough evidence to suggest that the size of bladderwort trap doors have a significant, positive relationship …

Tech Addiction: The Legal Limits Of A Growing Concept, Haneen Abu Ghanem

Marquette Intellectual Property & Innovation Law Review

None.

Stars, Statutes, And Sharing: Designing Global Ip Frameworks For Space-Based Discoveries, Ed Koellner

Marquette Intellectual Property & Innovation Law Review

As humanity stretches farther into space physically, commercially, and intellectually, contemporary legal frameworks are beginning to creak under the pressure. Intellectual property laws seem to be playing catch-up. Those laws were written for a world bound by borders and gravity, not for orbital labs, the Earth’s Moon, or AI-generated inventions drifting above the Earth.

This article takes a closer look at the increasingly complex intersection of space law, intellectual property, and artificial intelligence. This domain is no longer reserved for state-led missions or high-level treaties. Now and in the near future, a university lab or a private startup might be …

Treatment Sequence With Tebentafusp And Immune Checkpoint Inhibitors In Patients With Metastatic Uveal Melanoma And Metastatic Gna11/Gnaq Mutant Melanocytic Tumors, Florentia Dimitriou, Marlana Orloff, Erica Koch Hein, Phil Cheng, Isaac Hughes, Ester Simeone, Kamaneh Montazeri, Piyush Grover, Inderjit Mehmi, Camille Gerard, Caroline Gaudy-Marqueste, Jean-Jacques Grob, Olivier Michielin, Omid Hamid, Georgina Long, Ryan Sullivan, Ellen Kapiteijn, Douglas Johnson, Paolo Ascierto, Anthony Joshua, Richard Carvajal, Marcus Butler, Jessica Hassel, Reinhard Dummer

Kimmel Cancer Center Faculty Papers

BACKGROUND: Metastatic uveal melanoma (mUM) is rare. Immune checkpoint inhibitors (ICIs) have shown modest efficacy in mUM. Tebentafusp prolonged overall survival (OS) in a phase 3 study. We aimed to investigate the efficacy and safety of the sequence of tebentafusp and ICIs.

METHODS: Patients with HLA-A * 02:01 positive mUM, or metastatic GNA11/GNAQ mutant melanocytic tumors treated with tebentafusp followed by ICIs (group 1) or the inverse sequence (group 2) at any treatment line were retrospectively identified. The primary objective was OS rate at 2 years.

RESULTS: 131 patients were included; 51 in group 1 and 80 in group 2. …

Effects Of Liraglutide On The Gut Bacterial Community, Jami Anne Bull

Graduate Theses/Dissertations

Overweight and obesity are major health concerns associated with metabolic disorders and gut dysbiosis. Treatment options such as GLP-1 receptor agonists, like liraglutide, promote weight loss and improve insulin sensitivity; however, their impact on the gut bacterial community remains unclear. This study investigated the effects of liraglutide on the gut bacterial community of diet-induced obese (DIO) mice using Illumina DNA sequencing of the bacterial 16S rRNA gene. My study used twelve-week-old male C57BL/6J DIO mice. They were placed on a high-fat diet and treated with liraglutide (0.2 mg/kg) via daily subcutaneous injection for 14 Days. High-fat and low-fat diets control …

The Influence Of Flower-Microbe Interactions On Bumble Bee Behavior And Gut Microbiome Composition, Success Chiamaka Ekemezie

Graduate Theses/Dissertations

This thesis examines how microbial interactions in floral nectar and environmental variation influence bumble bee (Bombus spp.) foraging behavior and gut microbiome composition. Pollen germination experiments and behavioral assays in the lab tested whether Metschnikowia reukaufii (yeast) and Acinetobacter nectaris (bacteria) induce pollen germination and bursting in nectar and affect bee behavior subsequently. Both microbes reduced intact pollen, but bees did not alter flower preference. Furthermore, the study also assessed how floral and bee community composition shaped gut microbiome composition across six Missouri prairies. Using amplicon-sequenced fungal ITS and bacterial 16S rRNA genes, I found that fungal microbiome composition varied …

Cgrp Modulation Of Intracellular Calcium Levels In Trigeminal Ganglion Neurons And Glia, Nicole M. Nalley

Graduate Theses/Dissertations

Elevated levels of calcitonin gene-related peptide (CGRP) are implicated in migraine and TMD pathology, but its effects on the excitability state of Aδ and C-fiber neurons and glia have not been investigated. The goal of this study was to determine changes mediated by CGRP on intracellular calcium levels in trigeminal ganglion neurons and glia in response to depolarizing stimulation. Intracellular calcium levels were determined in Aδ and C-fiber neurons of primary trigeminal ganglion cultures obtained from neonatal Sprague Dawley rats using Fura-2 and fluorescent microscopy. Cells were left untreated or preincubated for 2 hours with CGRP, then incubated with the …

Spatial And Temporal Shifts In Bacterial Communities And Nutrient Removal In Two Springfield Wastewater Facilities, Rubina Sherchan

Graduate Theses/Dissertations

Bacterial communities play a central role in the biological nutrient removal and overall performance of wastewater treatment plants (WWTPs). However, very few is known about the spatial and seasonal dynamics of these communities. This study explores the spatial and seasonal variations in bacterial community composition across multiple treatment stages in two municipal WWTPs—Southwest (SWTP) and Northwest (NWTP)—in Springfield, Missouri. Using 16S rRNA gene amplicon sequencing, I analyzed 87 samples collected during summer and winter of 2024 from influent, sludge, aeration basins, clarifiers, and final effluent stages. I retrieved 16.4 million (summer) and 3.2 million (winter) sequences. Proteobacteria dominated both seasons …

Preventing Chemical And Biological Attacks: Enhanced Threats Requiring Enhanced Solutions, Alex Elliot Ottoni-Belval

Graduate Theses/Dissertations

Chemical weapons (CW) and biological weapons (BW) are becoming more dangerous for experts to employ and more accessible to amateurs. Despite CW and BW attacks throughout history that killed and maimed millions of people, the international community has failed to establish meaningful consequences to prevent them. Agreements such as the Chemical Weapons Convention and the Biological Weapons Convention vow to eliminate and reduce the effects of CWs and BWs but lack respected response precedents and capabilities. The limited and inconsistent enforcement has inadvertently allowed CWs and BWs to become plausible weapons, especially to gain asymmetric advantages, while modern developments exasperate …

Investigating Social Attenuation Of The Stress Response In Juvenile Northern Cottonmouths (Agkistrodon Piscivorus), Alyssa Owens

Graduate Theses/Dissertations

When social organisms encounter a stress-inducing event, the presence of a conspecific can dampen the physiological and/or behavioral response elicited by the stressor. This concept, termed ‘social buffering’, has been well documented in mammals, but there are few studies investigating this phenomenon in organisms traditionally perceived as asocial, including reptiles. However, the paradigm is shifting, and social behavior is now more widely recognized in reptiles, including snakes. In this study I tested whether juvenile Northern Cottonmouths (Agkistrodon piscivorous) experience social buffering by measuring changes in heart rate in snakes following an acute stressor with and without the presence …

Alpha-Synuclein Interaction With Gedunin, Tony Matundura Nyabayo

Graduate Theses/Dissertations

Parkinson’s disease (PD) and other Proteinopathies develop when α-synuclein misfolds and aggregates into toxic amyloids. While existing treatments for PD are primarily focused on managing its symptoms, a viable solution for slowing down the progress of Parkinson’s disease involves targeting the toxic α-synuclein amyloids. Gedunin, a natural inhibitor of heat shock protein 90, has been extensively used to treat malaria. Also, recent research investigations have shed light on its potential beyond malaria therapy, indicating that Gedunin may offer a possible solution for treating a variety of neurodegenerative diseases. Using plate-based assays, we examined how Gedunin influences α-synuclein fibrillation and its …

Lc-Ms Discriminates Between Effects Of Sulfur Nanoparticles Vs Molybdenum Disulfide Nanoparticles On Lettuce (Lactuca Sativa), Mary Fakunle

Graduate Theses/Dissertations

Nanotechnology is gaining attention in agriculture, majorly due to their potential benefits in farming practices. Sulfur and molybdenum disulfide nanoparticles are reported to enhance plant growth, protection, and nutrient delivery. However, there is a need to understand how nano-fertilizers affect the metabolites and nutritional benefits of plants. Here, lettuce was cultivated under greenhouse in one of three treatments: control, 50 ppm S NPs, and 20 ppm MoS2 NPs. A method was developed on Liquid Chromatography – Mass Spectrometry (LC-MS) instrument to quantify the concentration of amino acids and antioxidants in the harvested lettuce plants. Inductively Coupled Plasma – Mass Spectrometry …

Investigating The Presence Of Individuality And Behavioral Syndromes In Northern Cottonmouths (Agkistrodon Piscivorus), Joshua Lowy

Graduate Theses/Dissertations

Within the last 15 ̶20 years, research on behavioral syndromes has seen a surge in prominence and popularity in behavioral ecology, revealing that many animals exhibit individually consistent behavioral tendencies. Behavioral syndromes occur when behavioral traits are correlated with one another within the same context or across different contexts. Despite new appreciation for the ecological, evolutionary, and conservation implications of behavioral syndrome data, the taxonomic breadth of studies is uneven. Snakes are underrepresented within squamate reptiles and, among venomous species, only two rattlesnakes (Crotalus) have been investigated for evidence of behavioral syndromes. My study investigates individuality and the presence of …

A Mystery Of Microecology: Revealing The Ecological Relationship Between Allorhizobium Vitis And Missouri Native Grape Species, Hunter White

Graduate Theses/Dissertations

Crown gall disease can be very detrimental to grape and wine producers around the world by reducing vine vigor and yield. It is caused by the bacteria Allorhizobium vitis (A. Vitis) carrying a tumor inducing (Ti) plasmid. The A. vitis bacteria have been found in soil and cane tissues in vineyards around the world, and infect all cultivated grapes. However, there have been no reports of crown gall on native wild grapevines, and very little is known about the ecology of A. vitis in native ecosystems. I have sampled cambium tissues from three native wild grape species of …

Investigations Of Proteins Including Yellow Thermal Protein, Blue Thermal Protein, Lactoperoxidase, And Thyroid Peroxidase, Cammi Dargatz

Graduate Theses/Dissertations

This research investigated four different proteins: yellow thermal protein (YTP), blue thermal protein (BTP), lactoperoxidase (LPO), and thyroid peroxidase (TPO). YTP and BTP were both engineered from the extremely stable and non-aggregative thermal green protein (TGP) by mutating one residue each to obtain proteins with shifted spectra while preserving the stability properties observed in TGP. Site-directed mutagenesis (SDM) was utilized to construct YTP by mutating a histidine at residue 197 to a tyrosine (H197Y) and BTP by mutating a tyrosine at residue 67 to a histidine (Y67H). These proteins were expressed in E. Coli and purified with nickel and diethylaminoethyl …

Sensory Processing And Anxiety In Bipolar Disorder, Lyric Arvizu

Graduate Theses/Dissertations

Past research suggests that individuals with bipolar disorder often experience differences in sensory gating and sensory processing, and similar patterns have been observed in anxiety disorders. Given that anxiety is highly comorbid with bipolar disorder, the present study examined how these conditions relate to self-reported sensory processing differences. A total of 132 participants (88 female), including 43 with bipolar disorder, were included in the final sample. Anxiety symptomatology was measured via the State-Trait Anxiety Inventory (STAI) Form Y. Sensory processing questionnaires included the Sensory Gating Inventory (SGI) and the Adult Sensory Processing Scale (ASPS). Correlations, t-tests, a multivariate analysis of …

From The Battlefield To The Asylum: The Social Construction Of Disability In Italy, 1900 - 1950, Brandie Crisp Robb

Graduate Theses/Dissertations

The Italian social construction of disability reflects a complex interplay between social, religious, political, and religious influences present during the first half of the twentieth century. While Western European and American disability discourse remained similar, those influences unique to Italy resulted in the formation of two distinct disability discourses during the early twentieth century: that of the heroic physically disabled veteran who sacrificed everything for the good of Italy, and the eugenically unfit Italian associated with dependency and uselessness. By combining a theoretical framework that includes critical disability theory with historical methodology, the following thesis demonstrates how the emerging disability …

Help Or Hindrance? Humans As A Range Expanding And Reducing Force For Himalayan Black Bears In Nepal, Suman Shree Neupane

Graduate Theses/Dissertations

I explore the relationship between Himalayan black bears (HBBs) and humans by constructing niche models for HBBs considering both natural and human influences. This approach allowed me to assess the net impact of human activities on HBBs, which face challenges like habitat fragmentation, but also reap some benefits (like increased food availability). I examined HBB niche impacts from humans by quantifying and comparing the extent of HBB niche space when including versus excluding anthropogenic influences. Specifically, I estimated the natural HBB niche space by assuming no human alterations of the landscape and compared this natural range to the observed range …