Digital Commons Network^™

Improved Protein Splicing Through Viral Passaging, John Smetana

Scholars Week

Intervening proteins (inteins) are translated as subdomains within host proteins and removed through an intein-driven splicing reaction where the flanking sequences (exteins) are joined with a peptide bond. Previously, we developed a self-removing translation reporter for labeling Ebola virus (EBOV). In this reporter, an intein (RadA) containing the fluorescent protein ZsGreen (ZsG) is inserted within the EBOV protein VP30. Upon VP30-RadA-ZsG expression from the viral genome, RadA-ZsG is removed from VP30 through the protein splicing activity of RadA, generating functional, non-tagged VP30 and functional ZsGreen. While incorporation of our VP30-RadA-ZsG fusion reporter into recombinant EBOV (rEBOV- RadA-ZsG) resulted in an …

Biology And Damage Assessment Of Fall Army Worm (Spodoptera Frugiperda, Smith) Under Laboratory Condition, Pratibha Adhikari

Scholars Week

No abstract provided.

Depth Patterns Of Redear Sunfish Lepomis Microlophus In Kentucky Lake, Justin Graben

Scholars Week

Redear Sunfish Lepomis microlophus are a highly sought sportfish known for their size potential, but their ecological behavior in large reservoirs remain poorly understood. In this study, we looked at how Redear Sunfish Lepomis microlophus move vertically in Kentucky Lake. We used acoustic tags with depth sensors to measure the movement of Redear Sunfish both diurnally and seasonally within Kentucky Lake.

During the day, Redear Sunfish occupied significantly deeper waters compared to dawn, dusk, and night (p < 0.0001.). Many fish, including the species in the Lepomis genus, may have a preference for specific light levels for optimal foraging and predation avoidance, even if predation is not the primary concern at larger sizes. This daily pattern was consistent through most seasons, except on average the fish were deeper during the winter season (p < 0.0001).

Understanding the movement patterns of Redear Sunfish is essential for effective species management, especially as their popularity grows and they potentially compete with …

Microcanonical Analysis Of Semiflexible Homopolymers With Variable Bending Potential Width, Michael Gray

Scholars Week

I have developed an algorithm for finding phase transition in microcanonical data of simulations of homopolymers. These simulations model the behavior of homopolymers in an array of different conditions. This algorithm is generalized and can be applied to a variety of different systems. It works by taking repeated derivatives of the microcanonical entropy of the system and applying several filters, whose parameters are tuned algorithmically as well. Identification of phase transitions allows for the construction of a phase diagram.

The Moderating Role Of Aphantasia In The Link Between Emotion Regulation And Relationship Satisfaction, Brock Borchardt, Dilbur D. Arsiwalla

INSPIRE Student Research and Engagement Conference

The goal of this study was to examine if poor visual imagery (i.e., aphantasia) moderated the link between emotional regulation and relationship satisfaction. We had 314 participants report on a survey about visual imagery, emotional regulation, and relationship satisfaction. Our original hypothesis was supported using the overall emotion regulation scale as well as five of the six different subscales of emotion regulation. However, further research is needed to better understand the rationale behind why aphantasia moderates this relationship.

Where’D You Go, Ohio: Progressive Ohio Ballot Initiative Passage In A Republican Aligned State, Colin D. Swearingen, Elizabeth Stiles, Barrett Scheatzle

The Journal of Economics and Politics

We investigate the effects of cosmopolitan and traditional county features on the opposition to three successful liberal state-wide initiatives on abortion and recreational marijuana using county-level data. Cosmopolitan/traditional variables include urban/rural living patterns, college graduates, average income and religiosity while control variables include turnout, poverty, race, region, and union support. We observe the results of these three elections to see the relationship between vote shares and factors such as income, education, rural/urban, and religiosity, controlling for other factors. Utilizing OLS and GAM regression models, our results provide support for our hypothesis that there is a relationship between cosmopolitanism and votes …

Culex Quinquefasciatus Mosquitoes In The Abu Dhabi Emirate: Assessing Deltamethrin Insecticide Resistance And Associated Microbial Communities, Amgd Sayed Ismat

Thesis/ Dissertation Defenses

This study investigates the Culex quinquefasciatus is a major vector of West Nile virus, lymphatic filariasis, and arboviruses, which represent public health risk. Widespread application of pyrethroid insecticides has increased resistance in mosquito populations threatening the effectiveness of control strategies. This study aims to (1) assess deltamethrin resistance in Culex quinquefasciatus populations across the Abu Dhabi Emirate, (2) characterize the microbial communities associated with these mosquitoes. Mosquitoes from sites around the Abu Dhabi Emirate were collected and genomic DNA extracted for molecular analysis. PCR and allele-specific primers were used to detect knockdown resistance (kdr) mutations in the sodium channel gene, …

Herbicide Utilization For Regulation Of Botanical Composition Of Grass Swards, A Lackovic

IGC Proceedings (1977-2023)

In Czechoslovak conditions especially in Slovakia the botan­ical composition of grass swards is for different reasons getting worse and this means the decrease of forage value. As all those swards cannot be improved by complete renovation or by direct strip drilling of productive species into the sward, other possibilities are looked for. One for those is the utilization of herbicides combined with adequate fertilization. Retardation or control of unfavourable species destroy the existing competition which causes the different species succession in the sward. Our aim was to make a positive succession of species in the sward by herbicides utilization and …

Allelopathic Influence Of Certain Pasture Weeds, A E. Smith

IGC Proceedings (1977-2023)

Allelopathy, the direct or indirect effect of one plant on another through the production of chemical compounds that escape into the environment (Rice, 1974) occurs widely in natu­ral plant communities (Gressel and Holm, 1964), and is pos­tulated to be one mechanism by which weeds interfere with crop growth (Gressel and Holm, 1964; Bell and Koeppe, 1962; Muller, 1966). Probably the most significant consequence of allelopathy is the alteration of population densities that affect vegetation patterns in plant communities. The actual and potential roles of allelopathy in agriculture have been exten­sively reviewed (Putman and Duke, 1978; Rice, 1974; Rice, 1979). Generally, …

Economics Of Controlling Weeds Of Permanent Pasture, I Popay, N Barlow

IGC Proceedings (1977-2023)

In general, inedible weeds of permanent pasture reduce pas­ture dry matter available to stock in direct proportion to their cover (Doyle et al., 1984). Translating the effects of weeds on pasture to reductions in animal production is difficult because (i) pasture value can range from zero to the cost of purchased feed (ii) weed effects and pasture value can vary seasonally and interact and (iii) reductions in pasture availability at one time affect later growth (Auld et al., 1987). There have been few direct measurements of effects of pasture weeds on animal pro­ductivity, because of the co.sts involved. However, simulation …

Weed Development In Tropical Sown Pastures, G.E D. Tiley

IGC Proceedings (1977-2023)

The use of artificially sown pastures is relatively recent in the tropics. Grasses and legumes have been evaluated for their value to livestock as well as the more traditional use of resting arable land after cropping. Using the criteria of ease of estab­lishment, production under grazing and botanical stability, a number of species and mixtures were recommended for the humid areas of Uganda (Horrell and Tiley, 1970). Control of weed growth during early establishment and suppression of unsown species subsequently were found to be vital for the success of these tropical ley pastures. The results of monitoring weed development in …

Insect Pests As A Limiting Factor In Green Fodder Production In Lucerne, Their Control And Residual Persistence Of Insecticides, V J. Shivankar

IGC Proceedings (1977-2023)

The green fodder production in lucerne suffers serious loss of about 38 % caused by the insect pests like Empoasca sp., Therioaphis maculata Buck, Aphis craccivora Koch and Hypera variabilis Herbst. under the tropical agroclimatic zone of Bundelkhand-India (Shri Ram and Gupta, 1976). Of these H. variabilis is most serious with regular occurrence and world­wide distribution. No work has been reported on the control aspect of these pests in India, except a few on insecticidal evalu­ation (Shri Ram and Gupta, 1975) and the economic injury level of lucerne weevil (Gupta et al., 1982). Need of the studies on the chemical …

Sitona Hispidulus Larvaw As A Stress On Alfalfa Production, A A. Hower, K T. Leath

IGC Proceedings (1977-2023)

Sitona weevils are pests of various legumes throughout the world. In the United States, the clover root curculio, S. hispi­dulus (F.) is the major species associated with lucerne (alfalfa) Medicago sativa L. This insect has until recently remained obscure as a pest of this major forage crop. However, a recent trend in premature decline of alfalfa has prompted further investigation of this insect/legume interaction. Godfrey and Yeargan (1987) and Hill et al. (1969) indicated that S. hispi­dulus reduced alfalfa production. While adults S. hispidulus feeding on the foliage is of little consequence, it is the associ­ation of larvae with …

Resistance To Plant Parasitic Nematodes In Ryegrasses And White Clover, R Cook, K A. Mizen

IGC Proceedings (1977-2023)

Field and glasshouse experiments indicate that several nematodes damage ryegrasses and white clover (Cook and York, 1985). Populations of these nematodes under grassland are often at potentially damaging densities. Several nematode species of long-term grassland have co-evolved host relationsh­ips in which host resistance is likely to have been selected. This is of direct practical relevance to the control of three nematodes, widespread in England and Wales: the root-knot nematode (Meloidogyne naasi) on ryegrasses; and the stem nematode (Ditylenchus dipsaci) and cyst nematode (Heterodera trifolii) on white clover. Our objectives have been, first, to identify resistant …

The Effect Of Redlegged Earthmite (Halotydeus Destructor) On Legume Productivity, D J. Gillspie, D A. Nicholas

IGC Proceedings (1977-2023)

Redlegged earthmite (Halotydeus destructor) (RLEM) is a

serious pest of legume-based annual pastures in southern Australia. Observations throughout Australia show that every year they cause both mortality and growth retardation in emerging pastures in autumn, and are also responsible for reduced seed set in annual legumes in spring (Allen 1986, Norris, 1948). The most common legumes in areas where RLEM occur, are annual Trifolium species, especially T. subterraneum (subclover). However, the recent development of M edicago polymorpha and M. murex for use on acid soils together with the wider use of Ornithopus compressus, has resulted in alternative hosts for these …

Resistance In Cynodon Dactlyon To Spodoptera Frugiperda: Influence Of Strain And Dietary Conditioning, S S. Quisenberry

IGC Proceedings (1977-2023)

The most important warm-season perennial grass in the southern United States is bermudagrass (Cynodon dactylon [L.]). Fall armyworms (Spodopterafrugiperda [J.E. Smith]) are the most damaging insect pests of bermudagrass. Conse­quently, researchers have evaluated improved bermudagrass varieties for resistance to fall armyworm (Leuck et al., 1968; Lynch et al., 1983; Quisenberry and Wilson, 1985; Jamjanya and Quisenberry, 1988). Comparison of results from these studies have been contraditory, whereas Leuck et al., (1968) and Lynch et al., (1983) reported Tifton 292 to be extremely resistant (100 % larval mortality) to fall armyworm, Jamjanya and Quisenberry (1988) found it to have …

A Methodology For Evaluation Of Host Plant Resistance In Brachiaria To Spittlebug Species (Homoptera: Cercipidae), L Lapointe, G Arango, G Sotelo

IGC Proceedings (1977-2023)

Previously underutilized lowlands in tropical America are increasingly being used to produce meat and milk largely due to Brachiaria Griseb., an introducea genus of East African grass. Brachiaria is well adapted to infertile, acidic soils with high aluminium saturation. It is estimated than 16 million hectares have been planted to B. decumbens Stapf and B. humidicola (Rendle) Schweick in Latin America (Toledo and Nores, 1986). The various genera and species of spittlebugs native to the neotropics are the principal biotic factor limiting utilization of Brachiaria as forage in Latin America. Spittlebugs are capable of causing severe damage to susceptible …

Some Effects Of Diseases And Pests On Seed Production In White Clover, And Their Control, M J. Foulkes, B C. Clifford

IGC Proceedings (1977-2023)

The UK market for seed of white clover (Trifolium repens L.) cultivars is in the order of 1,000 t within an EEC total of some 3,500 t. Although high yields of around 500 kg ha-1 can be achieved, yields are extremely variable (Hides, Lewis and Mar­shall, 1984) and this may be due in part to pathogenic attack. The results reported here are of a field experiment where dis­eases and pests were controlled by pesticide applications in plots grown under two simulated seed production man­agements.

Feeding Sites Of Sitona Hispidulus Larvaw As Infection Foci For Fusarium Oxysporum F. Sp. Medicaginis In Alfalfa Roots, K T. Leath, A A. Hower

IGC Proceedings (1977-2023)

Fusarium wilt of alfalfa (Medicago sativa L.) was first described in 1927 (Weimer, 1928) in the United States and has since been reported from many other areas of the world. The causal agent is Fusarium oxysporum Sehl. f. sp. medicaginis (Weimer) Snyd. & Hans. Shoots of infected plants become yellow and wilt ; often only one side of the plant is affected. The outer root xylem tissue turns reddish brown, and infected plants usually die within one season (Leath et al., 1988). The fungus is soil-borne, survives in organic debris, and can persist up to 10 years as chla­mydospores …

Non-Chemical Control Of Some Disease And Pests In Red Fescue And Kentucky Bluegrass Seed Production, B Cagas

IGC Proceedings (1977-2023)

Diseases and pests are one of the main problems in grass seed production in Czechoslovakia. The silver top (white head) caused by the bug Leptopterna dolobrata L. and the ergot [Cla­viceps purpurea (Fr.) Tu!.] have an effect upon seed quantity and quality. In some years the leaf diseases (rusts, powdery mil­dew, spots) can play an important role in the seed ripening. The chemical control may leads to some problems. The effect of burning in two different periods (summer, spring) and the close cutting were compared in red fescue (Festuca rubra L.) and Kentucky bluegrass (Poa pratensi …

White Clover Yield, Quality, And Persistence : Insecticide, Fungicide, Nematode, And Virus Effects, G A. Pederson, G L. Windham, M M. Ellsbury, M R. Mclaughlin, R G. Pratt, G E. Brink

IGC Proceedings (1977-2023)

White clover (Trifolium repens L.) is the most important for­age legume for perennial pastures in the southeastern United States. Commonly, white clover stands decline within 2 to 3 years after seeding. This decline has been reported to be due to many factors including fungal diseases (Graham et al., 1961 ; James et al., 1980) insects (Graham et al., 1961 ; James et al., 1980), viruses (Kreitlow et al., 1957; Barnett and Gibson, 1977), and root-knot nematodes (Baxter and Gibson, 1959). No studies have evaluated effects of combinations or interac­tions of these factors on white clover growth in the field. …

La Lutte Contre Les Rouilles Du Ray-Grass Anglais Porte-Graine, H Clinkspoor, G Sicard

IGC Proceedings (1977-2023)

Les cultures de ray-grass anglais porte-graine couvrent envi­ron chaque annee en France 2800 hectares, soit un peu mains de 20 % des surfaces nationales toutes graminees confondues. Une enquete realisee sur l'etat sanitaire de ces cultures avait revele qu'elles sont souvent attaquees par des rouilles au debut de l'ete. Quatre rouilles peuvent ainsi etre rencontrees : la rouille cou­ronnee provoquee par Puccinia coronata est la plus frequente ; la rouille noire (P. graminis), la rouille brune (P. loliina) et la rouille jaune (P. striiformis) peuvent egalement se rencontrer. En conditions climatiques favorables, Jes cycles d'infection …

A Multi-Disciplinary Approach To Anthracnose Diseases Of Stylosanthes In Australia, D F. Cameron, S Chakraborty, R D. Davis, L A. Edye, J.A G. Irwin, J M. Manners, I B. Staples

IGC Proceedings (1977-2023)

Breeding Stylosanthes for resistance to anthracnose diseases in northern Australian pastures is complicated by extensive pathogenic variation in the fungal pathogen, Colletotrichum gloeosporiodes. We have put considerable emphasis on epidemiological studies in an attempt to devise optimal strategies for the use of resistance genes in our selection and breeding programs. In addition, techniques of molecular biology are being used to investigate prospects for the use of genes from novel sources and to study the genetics of virulence in the pathogen.

Breeding For Resistance To Bacterial Wilt Of Grasses (Xanthomonas Campestris Pv. Graminis), D Schmidt

IGC Proceedings (1977-2023)

Bacterial wilt of fodder grasses caused by Xanthomonas campestris pv. graminis was first described by Egli et al. (1975), and since then observed all over Europe and in New Zealand. The regular mowing of the crop offers ideal conditions for spreading of the pathogen. Several grass species are affected. The most severe yield losses occur in Lolium multiflorum, particularly in var. westerwoldicum. In extreme cases, all the plants can be killed during hot, dry summers. Gondran and Betin (1988) found up to 47 % of yield loss in the three cuts following inoculation of Italian rye-grass cultivars. Losses are less …

L’Anthracnose De La Luzerne Dans Le Maroc Oriental Resistance De Quelques Cultivars Et Lignees, Huguette Gosset, B M. Troeung, G Raynal

IGC Proceedings (1977-2023)

Dans le Maroc oriental (regions d'Oujda, Berkane, Ai:n Beni Mathar), la luzerne (Medicago sativa L.) ne peut etre cultivee que dans les zones irriguees, en raison de l'aridite du climat. Dans ces conditions, elle est exploitee pour le fourrage tous les 20 jours au printemps et en ete et tous les 30-40 jours en automne et en hiver. La variete largement utilisee actuellement est Moapa dont les cultures, lorsqu'elles sont saines, durent 5 ans. Des prospections nous ont montre que de nombreux pro­blemes sanitaires existent. C'est ainsi que l'anthracnose (Colle­totrichum trifolii Bain et Essary) apparait comme !'affection fongique predominante …

Diachorella Leaf Spot Of Prairie Milkvetch In Loess Plateau, China, Hou Tianjue, Wang Jiangfeng

IGC Proceedings (1977-2023)

Prairie Milkvetch (Astragalus adsurgens), an acclimatized good sand binder, soil and water conservation legume forage plant, cultivated widely in the arid and semi-arid districts of North China, is especially suitable for the Loess Plateau, China. Unfortunately in the mid Inner Mongolia, North Shaanxi Province and the eastern part of Gansu Province, it is often infected seriously with Diachorella leaf spot (Diachorella lathyri Fckl.). The disease primarily affects leaves, but some­times attacks petioles and stems. On infected leaflets first appear small black spots. As the spots increase, whole leaflet is blackened. Heavy infections kill leaflets and cause severe …

La Certicilliose De La Luzerne En France Mise Au Point Apres 15 Annees D’Observations, J Gondran

IGC Proceedings (1977-2023)

La verticilliose de la luzerne (Verticillium albo-atrum R.B.) fut determinee pour la premiere fois en France il y a une tren­taine d'annees. Depuis, nous avons etudie la repartition geo­graphique de la maladie (Gondran, 1976) et la biologie du parasite· (Gondran, 1977). Nous avons mis au point un test de selection permettant de creer des varietes resistantes (Gondran in Massenot et al., 1978). Nous avons commence une etude sur la variabilite du pouvoir pathogene du champignon (Gondran, 1985). Nous nous proposons de faire le point sur !es etudes sui­vantes : degats de la maladie, variabilite du pouvoir pathogene du …

Observations Sur La Dissemination D’Epichloe Typhina, L’Agent De La Quenouille Des Graminees Et Reflexions Sur Les Diverses Especes D’Acremonium, G Raynal

IGC Proceedings (1977-2023)

La quenouille des graminees est due a l'Ascomycete Epichloe typhina (Pers. ex Fr.) Tulasne. Des l'epiaison, Jes inflorescences malades sont emprisonnees clans un manchon blanc (stade ase­xue Sphacelia typhina = Acremonium typhinum), qui prend progressivement une couleur orangee, pour donner le symp­t6me typique de quenouille. Le stroma des quenouilles ren­ferme Jes loges peritheciales caracterisant le stade sexue Epichloe et produisant Jes ascospores responsables des conta­minations. De nombreuses graminees sont attaquees, dont le dactyle ( Dactylis glomerata L.), qui peut subir de serieux dom­mages en France clans Jes regions de production de semence. En effet, Jes fleurs ne peuvent pas, …

Mycoflora Response To Burning On Sand Prairie, S S. Dhillon, C Anderson

IGC Proceedings (1977-2023)

The rhizoplane and root interior support different popula­tions of microorganisms. A large number of these organisms are microfungi. Studies have shown that fire induced changes are favourable for some groups of microorganisms following grasslands fires. However, most studies have dealt with the effect of burning on surface and subsurface soil organisms but none have examined rhizoplane fungi. This study was conducted to examine little bluestem (Schiz­achyrium scoparium), a native prairie grass species, biomass production and associated vesicular-arbuscular mycorrhizae (V AM) and rhizoplane mycoflora and their response to burning.

Recent Effects Of Acremonium Coenophialum On The Performance Of Beef Cattle Grazing Festuca Arundinacea, H A. Fribourg, J B. Mclaren, A B. Chestnut, J C. Waller

IGC Proceedings (1977-2023)

Infection of tall fescue (F. arundinacea Schreb.) plants by the endophytic fungus A. coenophialum Morgan-Jones & Garns is associated with substantial economic reduction in grazing ani­mal performance (Bacon and Siegel, 1988 ; Bush and Burrus, 1988 ; Stuedemann and Hoveland, 1988). This problem is serious in the transition zone of the eastern U.S.A., affecting over 12 million ha of pasture land.