Digital Commons Network^™

Plasma Proteomic Biomarkers Of Physical Frailty In Heart Failure: A Propensity Score Matched Discovery-Based Pilot Study, Quin Denfeld, Noelle Pavlovic, Christopher Lee, Jon Jacobs, Mary Roberts Davis, Samantha Powell, Marina Gritsenko, Susan Joseph, Beth Habecker

Division of Cardiology Faculty Papers

BACKGROUND: Physical frailty is highly prevalent in heart failure (HF), but we lack an understanding of the underlying pathophysiology. Proteomic evaluation of plasma samples may elucidate potential mechanisms and biomarkers of physical frailty in HF.

OBJECTIVES: We aimed to identify plasma proteomic biomarkers that are differentially expressed between physically frail and non-physically frail adults with HF.

METHODS: This was a secondary analysis of a subset of data and plasma samples from a study of frailty among patients with New York Heart Association (NYHA) Functional Classification I-IV HF. Physical frailty was measured using the Frailty Phenotype Criteria. Propensity score matching was …

Volumetric Changes In Cerebellar Transverse Zones: Age And Sex Effects In Health And Neurological Disorders, Farshid Ghiyamihoor, Payam Paymani, Jarrad Perron, Azam Asemi-Rad, Mehdi Marzban, Aashka Mohite, Karen Ardila, Bara Aljada, Asghar Marzban, Mehnosh Toback, Sherif Eltonsy, Ji Hyun Ko, Tabrez J Siddiqui, Christopher J Steele, Jiming Kong, Mario Manto, M Ethan Macdonald, Jason S Gill, Roy V Sillitoe, Fuat Balcı, Iman Beheshti, Hassan Marzban

Duncan NRI Faculty and Staff Publications

Cerebellar volumetric changes are intricately linked to aging, with distinct patterns across its transverse zones, the functional subdivisions characterized by unique cytoarchitectural and connectivity profiles. Despite research efforts, the cerebellar aging process in health and neurological disorders remains poorly understood. In this study, we investigated the effects of age and sex on total cerebellum, transverse zone, and lobule volumes using MRI data from over 45,000 participants compiled from six neuroimaging datasets. We also propose a framework for estimating cerebellum age as an indicator of cerebellar health. Significant age‐dependent volume reductions were observed across transverse zones, with the …

Comprehensive Assessment Of Initial Adaptation Of Extended-Spectrum Β-Lactamase-Positive St131 Escherichia Coli To Carbapenem Exposure, William C Shropshire, Xinhao Song, Jordan Bremer, Seokju Seo, Susana Rodriguez, Selvalakshmi Selvaraj Anand, An Q Dinh, Micah M Bhatti, Anna Konovalova, Cesar A Arias, Awdhesh Kalia, Yousif Shamoo, Samuel A Shelburne

Faculty, Staff and Student Publications

Background: It remains unclear how high-risk Escherichia coli lineages, like sequence type (ST) 131, initially adapt to carbapenem exposure in their progression to carbapenem resistance.

Methods: Carbapenem mutation frequency was measured in multiple subclades of extended-spectrum β-lactamase (ESBL)-positive ST131 clinical isolates using a fluctuation assay followed by whole genome sequencing (WGS) characterization. Genomic, transcriptomic, and porin analyses of the ST131 C2/H30Rx isolate MB1860, under prolonged, increasing carbapenem exposure was performed using 2 experimental evolutionary platforms to measure fast versus slow adaptation.

Results: All 13 ESBL-positive ST131 strains selected from a diverse (n = 184) ST131 bacteremia cohort had detectable ertapenem …

Integrated Analysis Of Molecular And Clinical Features Associated With Overall Survival In Melanoma Patients With Brain Metastasis, Swaminathan Kumar, Meredith S Pelster, Merve Hasanov, Renato A Guerrieri, Courtney W Hudgens, Debora A Ledesma, Fuchenchu Wang, Grant M Fischer, Julie M Simon, Lauren E Haydu, Kalman V Katlowitz, Y N Vashisht Gopal, Jennifer L Mcquade, Lawrence N Kwong, Jason T Huse, Alexander J Lazar, Michael T Tetzlaff, Jeffrey E Gershenwald, Aron Y Joon, Ken Chen, Ziyi Li, Prahlad T Ram, Sherise D Ferguson, Michael A Davies

Faculty, Staff and Student Publications

Melanoma brain metastases (MBMs) are diagnosed in up to 60% of metastatic melanoma patients. Previous studies have identified clinical factors that correlate with overall survival (OS) after MBM diagnosis. However, molecular and immune features associated with OS are poorly understood. An improved understanding of the molecular and immune correlates of OS could provide insights into MBM patient outcomes and guide therapeutic development. Thus, we analyzed clinical features and outcomes of 74 melanoma patients who underwent surgical resection (via craniotomy) between 1991 and 2015 at our institution with RNA-seq data generated from their MBMs. The median post-operative OS was 8.6 months …

Single-Cell Analyses Reveal A Functionally Heterogeneous Exhausted Cd8+ T-Cell Subpopulation That Is Correlated With Response To Checkpoint Therapy In Melanoma, Kelly M Mahuron, Osmaan Shahid, Prachi Sao, Clinton Wu, Alexandra M Haugh, Laura A Huppert, Lauren S Levine, Margaret M Lowe, Michael Alvarado, Markee Micu, Katy K Tsai, Melissa Chow, Meromit Singer, Jason M Schenkel, Arlene H Sharpe, Michael D Rosenblum, Kristen E Pauken, Adil I Daud

Faculty, Staff and Student Publications

PD-1 pathway inhibitors have revolutionized cancer therapy. However, most patients do not durably benefit, highlighting the need for biomarkers to stratify patients as responders or nonresponders. Although CD8+ tumor-infiltrating lymphocytes (TIL) have been associated with immune checkpoint therapy response, there is no consensus on which CD8+ TIL subpopulations have the most prognostic value. Preclinical studies have focused on progenitor-like exhausted CD8+ T cells (TPEX) because TPEX proliferate more in response to PD-1 inhibitors than other exhausted T-cell (TEX) subpopulations. However, immune checkpoint inhibitor treatment drives TPEX differentiation into other TEX populations that can mediate antitumor immunity. These data complicate the …

Exploring Non-Traditional Yeast For Flavor Innovation In Non-Alcoholic Beer, Andrew Maust, Rahul Sen, Scott Lafontaine

Food Science Faculty Publications and Presentations

The demand for nonalcoholic beer (NAB) is growing, with nontraditional yeast strains emerging as valuable tools for brewers to craft distinct NABs. However, independent information about the performance of these commercially available yeasts remains limited. In this study, 11 nontraditional yeast strains were evaluated at two wort nutrient levels to assess their impact on beer chemistry, sensory attributes, and volatile profiles. Basic fermentation metrics were monitored, followed by descriptive sensory analysis and GC-MS/MS quantification of volatile compounds. Results revealed significant differentiation among yeast strains with unique aroma and flavor profiles driven by variations in ester production, fusel alcohol synthesis, and …

Preservation And Integrity Of Laboratory Solutions: Understanding The Impact Of Storage Practices On Chemical Stability And Experimental Accuracy, Azza Al Jahdhami

Honors Theses

Chemical storage methods represent a key matter ignored within labs, educational sites, workplaces, and even households. Many people, including various students and instructors within the chemistry field, are unaware of the proper methods for storing and disposing of common chemicals. This study aims to understand the effects of storage practices on chemical stability and experimental accuracy, dealing with the following questions: What kind of effects result from improper chemical disposal? How can improper storage affect the overall chemical effectiveness? Why is sink disposal of many solutions discouraged, and why is waste container disposal always necessary?

Through a literature review, this …

Investigating Cold Homicide Cases In Michigan: Combining Systematic Research With Experiential Insight, Jaqueline Chavarria

Honors Theses

According to the Michigan State Police (2023), murder/non-negligent manslaughter, commonly known as homicide, is the “willful (non-negligent) killing of one human being by another.” The Murder Accountability Project suggests there could be over 20,000 unsolved homicide cases in Michigan between 1965 and 2023 (2024). Despite the prevalence of unsolved cases, scholarly analysis on the topic is limited. This study examines solvability factors, also known as clearance factors, for cold homicide cases in Michigan by conducting a systematic literature review with news publications. Additionally, this study incorporates experiential insight, offering a holistic exploration of solvability factors. Using a systematic approach, 15 …

Morphogenic, Molecular And Cellular Adaptations For Unidirectional Airflow In The Chicken Lung, Kamryn N Gerner-Mauro, Lisandra Vila Ellis, Guolun Wang, Richa Nayak, Peter Y Lwigale, Ross A Poché, Jichao Chen

Faculty, Staff and Students Publications

Unidirectional airflow in the avian lung enables gas exchange during both inhalation and exhalation. The underlying developmental process and how it deviates from that of the bidirectional mammalian lung are poorly understood. Sampling key developmental stages with multiscale 3D imaging and single-cell transcriptomics, we delineate morphogenic, molecular and cellular features that accommodate the unidirectional airflow in the chicken lung. Primary termini of hyper-elongated branches undergo proximal-short and distal-long fusions, forming parabronchi for air conduction. Through the parabronchial smooth muscle, neoform termini extend radially to form gas-exchanging alveoli. Supporting this radial alveologenesis, branch stalks halt their proximalization, defined by SOX9-SOX2 transition, …

Mri Distance Measures As A Predictor Of Subsequent Clinical Status During The Preclinical Phase Of Alzheimer's Disease And Related Disorders, Xinyi Zhang, John C Morris, Tammie L S Benzinger, Et Al.

2020-Current year OA Pubs

Brain atrophy over time, as measured by magnetic resonance imaging (MRI), has been shown to predict subsequent cognitive impairment among individuals who were cognitively normal when first evaluated, indicating that subtle brain atrophy associated with Alzheimer's disease (AD) may begin years before clinical symptoms appear. Traditionally, atrophy has been quantified by differences in brain volume or thickness over a specified timeframe. Research indicates that the rate of atrophy varies across different brain regions, which themselves exhibit complex spatial and hierarchical organizations. These characteristics collectively emphasize the need for diverse summary measures that can effectively capture the multidimensional nature of degeneration. …

Lineage Tracing And Single-Cell Rna Sequencing Reveal A Common Transcriptional State In Breast Cancer Tumor-Initiating Cells Characterized By Ifn/Stat1 Activity, Eric P Souto, Ping Gong, John D Landua, Ramakrishnan Rajaram Srinivasan, Abhinaya Ganesan, Lacey E Dobrolecki, Stephen C Purdy, Xingxin Pan, Michael Zeosky, Anna Chung, S Stephen Yi, Heide L Ford, Michael T Lewis

Faculty, Staff and Students Publications

A tumor cell subpopulation of tumor-initiating cells (TIC) or "cancer stem cells" is associated with therapeutic resistance, as well as both local and distant recurrences. Signal transducer and activator of transcription (STAT) activity is elevated in TICs in claudin-low models of human triple-negative breast cancer, which enables enrichment of TICs using a STAT-responsive reporter. Lineage tracing of TICs as they undergo cell state changes could enable a better understanding of the molecular phenotypes of TIC and uncover strategies to selectively target TICs. In this study, we developed a STAT-responsive lineage-tracing system and used it in conjunction with the original reporter …

Distinguishing Multisystem Inflammatory Syndrome In Children From Typhus Using Artificial Intelligence: Mis-C Versus Endemic Typhus (Ai-Met), Angela Chun, Abraham Bautista-Castillo, Isabella Osuna, Kristiana Nasto, Flor M Munoz, Gordon E Schutze, Sridevi Devaraj, Eyal Muscal, Marietta M De Guzman, Kristen Sexson Tejtel, Tiphanie P Vogel, Ioannis A Kakadiaris

Faculty, Staff and Students Publications

Background: The pandemic emergent disease multisystem inflammatory syndrome in children (MIS-C) following coronavirus disease-19 infection can mimic endemic typhus. We aimed to use artificial intelligence (AI) to develop a clinical decision support system that accurately distinguishes MIS-C versus endemic typhus (MET).

Methods: Demographic, clinical, and laboratory features rapidly available following presentation were extracted for 133 patients with MIS-C and 87 patients hospitalized due to typhus. An attention module assigned importance to inputs used to create the 2-phase AI-MET. Phase 1 uses 17 features to arrive at a classification manually (MET-17). If the confidence level is not surpassed, 13 additional features …

Label-Free Evaluation Of Mouse Embryo Quality Using Time-Lapse Bright Field And Optical Coherence Microscopy, Fei Wang, Senyue Hao, Kibeom Park, Ali Ahmady, Chao Zhou

2020-Current year OA Pubs

The selection of high-quality embryos is essential to enhance the implantation rate for in vitro fertilization (IVF). Optical coherence microscopy (OCM) can noninvasively provide three-dimensional (3D) high-resolution imaging of developing embryos. The revealed microstructures can be used for accurate embryo evaluation. Here, we acquire time-lapse 3D OCM images with co-registered bright-field imaging on mouse embryo development from the one-cell stage to the fully hatched blastocyst inside an incubator. Our results demonstrate the capability of OCM to detect structural features of the developing embryos. The second and third embryonic cell cycles are indicated to be associated with blastocyst formation and the …

Using Human Computation And Harmonic Expansions For Characterization And Design Of Grain Boundary Networks, Christopher W. Adair

Theses and Dissertations

Grain Boundary Networks (GBNs) are a high-dimensional feature in polycrystalline microstructural materials that are formed from the crystallographic degrees of freedom. This feature affects multiple material properties such as diffusivity, fracture, elasticity, heat transfer, and others. The link between the crystallography and the material properties is called the structure-property linkage, and allows for simulation and design of materials. While it is possible to use the structure-property linkage to design materials for better performance, the high-dimensional nature of the interconnected grain boundaries preclude the use of many common optimization algorithms for efficiency or local minima considerations. This work shows how the …

Synapsin Condensation Is Governed By Sequence-Encoded Molecular Grammars, Christian Hoffmann, Kiersten M Ruff, Irina A Edu, Min Kyung Shinn, Johannes V Tromm, Matthew R King, Avnika Pant, Hannes Ausserwöger, Jennifer R Morgan, Tuomas P J Knowles, Rohit V Pappu, Dragomir Milovanovic

2020-Current year OA Pubs

Multiple biomolecular condensates coexist at the pre- and post- synapse to enable vesicle dynamics and controlled neurotransmitter release in the brain. In pre-synapses, intrinsically disordered regions (IDRs) of synaptic proteins are drivers of condensation that enable clustering of synaptic vesicles (SVs). Using computational analysis, we show that the IDRs of SV proteins feature evolutionarily conserved non-random compositional biases and sequence patterns. Synapsin-1 is essential for condensation of SVs, and its C-terminal IDR has been shown to be a key driver of condensation. Focusing on this IDR, we dissected the contributions of two conserved features namely the segregation of polar and …

Immunotherapeutic Strategies In Head And Neck Cancer: Challenges And Opportunities, Xia Liu, R. Alex Harbison, Mark A. Varvares, Sidharth V. Puram, Guangyong Peng

2020-Current year OA Pubs

HNSCC remains a substantial health issue, with treatment options including surgery, radiation, and platinum-based chemotherapy. Unfortunately, despite progress in research, only modest gains have been made in disease control, with existing treatments resulting in significant functional and quality-of-life issues. The introduction of immunotherapy in the treatment of HNSCC has resulted in some improvements in outlook for patients and is now standard of care for populations with both recurrent and metastatic disease. However, despite the early successes, responses to immune checkpoint inhibition (ICI) remain modest to low, approaching 14%-22% objective response rates. Challenges to the effectiveness of ICI and other immunotherapies …

Atm-Dependent Dna Damage Response Constrains Cell Growth And Drives Clonal Hematopoiesis In Telomere Biology Disorders, Christopher M. Sande, Karolyn A. Oetjen, Daniel C. Link, Andrew E. Gelman, Laneshia K. Tague, Et Al.

2020-Current year OA Pubs

Telomere biology disorders (TBDs) are genetic diseases caused by defective telomere maintenance. TBD patients often develop bone marrow failure and have an increased risk of myeloid neoplasms. To better understand the factors underlying hematopoietic outcomes in TBD, we comprehensively evaluated acquired genetic alterations in hematopoietic cells from 166 pediatric and adult TBD patients. Of these patients, 47.6% (28.8% of children, 56.1% of adults) had clonal hematopoiesis. Recurrent somatic alterations involved telomere maintenance genes (7.6%), spliceosome genes (10.4%, mainly U2AF1 p.S34), and chromosomal alterations (20.2%), including 1q gain (5.9%). Somatic variants affecting the DNA damage response (DDR) were identified in 21.5% …

Psychrotrophic Antarctic Marine Bacteria As Potential Reservoirs For Novel Antimicrobial Genes, Kudzai Hwengwere, Grant G. January, Kerry L. Howell, Lloyd S. Peck, Mathew Upton, Melody S. Clark

School of Biomedical Sciences

Antarctica is a very cold, isolated continent surrounded by frozen seas, yet these extreme environmental conditions have not restricted life and diversity in the sea. The marine environment is seasonally highly productive and harbours diverse and abundant communities of organisms, with many endemic species occurring nowhere else in the world. Such communities and their associated microbiomes are increasingly recognized as an unexplored source of novel antimicrobial products. Hence, the major aim of this study was to examine the antimicrobial potential of bacteria cultured from eight Antarctic marine invertebrate species, while gathering data on Antarctic microbial thermal and salinity tolerances. All …

Prm1 Influences Mating And Virulence In Cryptococcus Neoformans, Polycarp Amosun

Liberty University Research Week

Cryptococcus neoformans, an opportunistic pathogen, relies on homeostasis, environmental stability, and adaptability for its growth and survival. C. neoformans primarily infects immunocompromised individuals, increasing the propensity of invading the central nervous system. The most affected are individuals with HIV/AIDs. Recently, the World Health Organization classified C. neoformans as a Critical Priority fungal pathogen due to its widespread presence and high mortality rate. The ability to adapt to changes in environmental pH is one of the key factors contributing to C. neoformans pathogenicity. The gene CNAG_05866 was recently identified by researchers at Duke University via a mutagenesis screen to identify genes …

The Coffee Connection: Anti-Diabetic Potential Of Coffee-Derived Compounds And Their Underlying Cellular And Microbial Mechanisms, Emily Bayliss

Liberty University Research Week

Type II Diabetes (T2D) is characterized by chronically elevated fasting blood glucose resulting from insulin resistance. Epidemiologically, there is an inverse relationship between habitual coffee consumption and risk of T2D (1-3). While this relationship warrants further study, compounds naturally occurring in coffee may possess anti-diabetic potential. Matairesinol (MA), a coffee-derived polyphenol, has been shown to be effective in mitigating weight gain and fat mass accrual which are associated with T2D (4). Enterolactone (ENL), the metabolite of MA, has demonstrated the ability to act on L6 myotubes increasing basal glucose uptake while hindering triacylglycerol uptake in HEPA1-6 cells as well as …

Curcumin Promotes The Inhibition Irs-1 S1101 Phosphorylation While Restoring Insulin Signaling And Phosphorylation Of Pras 40 And Rps6., Rachael De Klerk, Davis Raz, John Zelenka

Liberty University Research Week

The search for natural compounds that elicit medicinal effects has increased over the years as synthetic compounds often carry adverse side effects. p300, an acetyltransferase protein recently found to negatively impact the insulin signaling pathway when overexpressed, has often been targeted in research using a synthetic compound called C646. However, curcumin, a natural compound, has been shown to also inhibit p300. Curcumin has been theorized as a potential treatment for diabetes, however the connection between its ability to inhibit the excessive expression of p300 seen when consuming a high fat diet and its ability to restore insulin signaling has never …

The Jacksons' Debate, Marcus Vinicius Neves

Vernacular: New Connections in Language, Literature, & Culture

The Jacksons’ Debate” explores ethical dilemmas through an alien civilization considering human consumption. This philosophical sci-fi story examines sentience, moral responsibility, and societal progress from a unique perspective. Blending dark humor with social commentary, it challenges readers to reflect on human behavior and ethical decision-making in the face of necessity and scientific advancement.

The Therapeutic Effects Of A High-Velocity Resistance Training Intervention For Chemotherapy-Induced Polyneuropathy, Mariah Gleeson, Lori Boright, Elise C. Brown, Deborah Doherty

Oncology Rehabilitation in Practice

Purpose: Chemotherapy-induced polyneuropathy, also referred to as chemotherapy-induced peripheral neuropathy (CIPN), is a dose-dependent, neurotoxicity from known neuropathic agents (e.g., taxanes, platinums, and vinca alkaloids) causing damage to the peripheral nervous system. CIPN can present as sensory, motor, or autonomic symptoms, such as muscle weakness and balance impairments. Currently, there are no treatment or prevention methods for CIPN. The purpose of this case series was to assess the therapeutic effects of a high velocity resistance training (HVRT), or power training, in women diagnosed and treated for cancer, who presented with CIPN by measuring balance, functional performance, neuropathy, quality of life …

From Microplastic Impacts To Biodegradation Solutions: Investigating Microplastic Contamination, Microbial Responses, And Efficacy Of Synthetic Microbial Consortia In Plastic Degradation, Rener Sahot De Jesus

Thesis/ Dissertation Defenses

Plastics, including microplastics, pose significant environmental threats due to their widespread presence and harmful ecological effects. This dissertation present three distinct, yet interconnected studies. First, a study on microplastic contamination in urban recreational parks was conducted in Al Ain, UAE. Results showed that low-density microplastics were present in 87% of soil samples, with FTIR analysis identifying synthetic polymers such as PE and PP. Additionally, these microplastics were found to negatively affect soil pH and moisture levels, emphasizing the need for enhanced monitoring and management strategies. Second, the effects of ground microplastics on Photobacterium leiognathi spp., a marine luminous bacterium, were …

The Shorthorn: May 12, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.

The Shorthorn: May 19, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.

The Shorthorn: April 15, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.

The Shorthorn: April 14, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.

The Shorthorn: April 7, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.

The Shorthorn: March 31, 1942

The Shorthorn Newspaper Collection: 1940s

No abstract provided.