Digital Commons Network^™

Effect Of Very Preterm Delivery On Outcomes Following In Utero Spina Bifida Repair, Michael V Zaretsky, Jagruti Anadkat, Kelly A Bennett, Yair J Blumenfeld, Chris Buchanan, Caitlin Clifford, Timothy Crombleholme, Samer Elbabaa, Stephen P Emery, William H Goodnight, Hanmin Lee, Joseph B Lillegard, Foong-Yen Lim, Francois I Luks, Virginia Lijewski, Jena L Miller, Ueli Moehrlen, Julie Moldenhauer, Anita J Moon-Grady, Mauro Schenone, Daniel W Skupski, Aimen F Shaaban, Kuojen Tsao, Tim Van Mieghem, Amy J Wagner

Faculty, Staff and Student Publications

Objective: In utero fetal spina bifida repair decreases the risk for cerebrospinal fluid (CSF) diversion including ventriculoperitoneal (VP) shunt placement, reverses hindbrain herniation, and improves motor function with a higher likelihood of independent ambulation. Unfortunately, in utero surgery can be complicated by preterm delivery. The goal of this study was to compare pregnancy and neonatal outcomes between individuals who delivered very preterm, and those who delivered later in gestation following in-utero spina bifida repair.

Methods: Data from the North American Fetal Therapy Network (NAFTNet) Fetal Myelomeningocele Consortium (fMMC) registry collected from 2011 to 2024 were analyzed. Three cohorts of patients …

Evidence For Biomolecular Condensates Formed By The Escherichia Coli Matp Protein In Spatiotemporal Regulation Of The Bacterial Cell Division Cycle, Inés Barros-Medina, Miguel Ángel Robles-Ramos, Marta Sobrinos-Sanguino, Juan Román Luque-Ortega, Carlos Alfonso, William Margolin, Germán Rivas, Begoña Monterroso, Silvia Zorrilla

Faculty, Staff and Student Publications

An increasing number of proteins involved in bacterial cell cycle events have been recently shown to form biomolecular condensates important for their functions that may play a role in development of antibiotic-tolerant persister cells. Here we report that the E. coli chromosomal Ter macrodomain organizer MatP, a division site selection protein coordinating chromosome segregation with cell division, formed biomolecular condensates in crowding cytomimetic systems preferentially localized at the membrane of microfluidics droplets. Condensates were antagonized and partially dislodged from the membrane by DNA sequences recognized by MatP (matS), which partitioned into them. FtsZ, a core component of the division machinery …

Multiparametric Mri Scoring System Of The Pancreas For The Diagnosis Of Chronic Pancreatitis, Temel Tirkes, Dhiraj Yadav, Darwin L Conwell, Xuandong Zhao, Anil K Dasyam, Vivek Gowdra Halappa, Aashish Patel, Zarine K Shah, Jordan Swensson, Naoki Takahashi, Sudhakar Venkatesh, Ashley Wachsman, Liang Li, Kristofer Jennings, Yunlong Yang, Phil A Hart, Stephen J Pandol, Walter G Park, Santhi Swaroop Vege, Mark Topazian, Paul R Territo, Scott A Persohn, Dana K Andersen, Evan L Fogel, Consortium For The Study Of Chronic Pancreatitis, Diabetes, And Pancreatic Cancer (Cpdpc)

Faculty, Staff and Student Publications

Background: Ductal features alone may not offer high diagnostic sensitivity or most accurate disease severity of chronic pancreatitis (CP).

Purpose: Diagnose CP based on multiparametric MRI and MRCP features.

Study type: Prospective.

Population: Between February 2019 and May 2021, 46 control (23 males, 49.3 ± 14.1 years), 45 suspected (20 males, 48.7 ± 12.5 years), and 46 definite (20 males, 53.7 ± 14.6 years) CP patients were enrolled at seven hospitals enrolled in the MINIMAP study. CP classification was based on imaging findings and clinical presentation.

Field strength and sequences: 1.5 T. T1-weighted (T1W) spoiled gradient echo, T1 map with …

Compartmental Disaggregation: Bridging Simulation And Sampling Methods For Synthetic Population Data Generation, Dylan Mack

McKelvey School of Engineering Graduate Student Theses & Dissertations

As agent-based models (ABMs) grow increasingly widespread in public health, their associated challenges have become all the more significant. Lauded for their ability to capture population heterogeneity, nonlinear dynamics, and emergent behaviors, disease ABMs are also computationally expensive and often require detailed inputs that describe each agent at the individual-level, known as synthetic population data. Current approaches for synthetic population data generation generally fall into one of two categories: sampling or simulation. These methods are both feasible only under restricted conditions and suffer from challenges surrounding data availability and computing power. This thesis proposes compartmental disaggregation, an intermediate method for …

Pregnancy Care Integrating Translational Science, Everywhere (Precise): A Prospective Cohort Study Of African Pregnant And Non-Pregnant Women To Investigate Placental Disorders – Cohort Profile, Rachel Craik, Joseph Akuze, Marie-Laure Volvert, Hannah Blencowe, Moses Mukhanya, Angela Koech, Grace Mwashigadi, Marleen Temmerman, Marvin Ochieng, Emily Mwadime

Centre of Excellence in Women and Child Health

Purpose: The Pregnancies' Care Integrating translational Science, Everywhere Network was established to investigate specific placental disorders (pregnancy hypertension, preterm birth, fetal growth restriction and stillbirth) in sub-Saharan Africa. We created a repository of clinical and social data with associated biological samples from pregnant and non-pregnant women. Alongside this, local infrastructure and expertise in the field of maternal and child health research were enhanced.

Participants: Pregnant women were recruited in participating health facilities in The Gambia, Kenya and Mozambique at their first antenatal visit or at the time a placental disorder was diagnosed (Kenya and The Gambia only). Follow-up study …

Rotation-Adaptive Point Cloud Domain Generalization Via Intricate Orientation Learning, Bangzhen Liu, Chenxi Zheng, Xuemiao Xu, Cheng Xu, Huaidong Zhang, Shengfeng He

Research Collection School Of Computing and Information Systems

The vulnerability of 3D point cloud analysis to unpredictable rotations poses an open yet challenging problem: orientation-aware 3D domain generalization. Cross-domain robustness and adaptability of 3D representations are crucial but not easily achieved through rotation augmentation. Motivated by the inherent advantages of intricate orientations in enhancing generalizability, we propose an innovative rotation-adaptive domain generalization framework for 3D point cloud analysis. Our approach aims to alleviate orientational shifts by leveraging intricate samples in an iterative learning process. Specifically, we identify the most challenging rotation for each point cloud and construct an intricate orientation set by optimizing intricate orientations. Subsequently, we employ …

Predicting Which Genes Will Respond To Transcription Factor Perturbation, Eric L. Jia

McKelvey School of Engineering Graduate Student Theses & Dissertations

A fundamental issue in mapping regulatory networks between transcription factors and their target genes is the poor overlap between the set of genes bound by a given transcription factor (TF), and the set of genes that are differentially expressed after knocking out or overexpressing the same TF. We began with the hypothesis that to predict whether a gene will respond to perturbation of a TF, it is important to not only consider whether that TF is bound at the gene’s promoter, but also whether other TFs bind at the same promoter.

In this work, we propose a novel modeling procedure …

The Prospects Of The High Seas Treaty Decisively Reducing The Negative Biodiversity Impacts Of Distant Water Fishing Operations, Shirley V. Scott, Nengye Liu

Research Collection Yong Pung How School Of Law

International fisheries law has so far proven inadequate to prevent over-fishing on the high seas, a key cause of biodiversity loss in areas of the ocean beyond national jurisdiction (ABNJ). Negotiation of the High Seas Treaty (Agreement under the United Nations Convention on the Law of the Sea on the Conservation and Sustainable Use of Marine Biological Diversity of Areas beyond National Jurisdiction or BBNJ Agreement) afforded an opportunity to respond decisively to the worsening problem. The Treaty was concluded in 2023. It is yet to enter into force. Preparations and negotiations for the Treaty spanned some 20 years. By …

Effects Of Different Blue Light Conditions On Saos-2 Cell Viability, Differentiation, And Mineralization, Jamielyn Alcantara Jarvis

Theses and Dissertations

Photobiomodulation (PBM) is a low-intensity light therapy that uses non-ionizing forms of energy to induce a directed cellular response. Prior work has investigated the effects of blue light exposure at energy densities ranging from 1 J/cm2 to 10 J/cm2 on osteogenic differentiation of human gingival mesenchymal stem cells (hGMSCs), human dental pulp stem cells (hDPSCs), and stem cells from the apical papilla (SCAPs) and shown promising outcomes at 4 J/cm2 and then having a detrimental impact at higher energy densities. One key caveat in prior studies is that the blue light energy density was modulated by changing the light exposure …

Are You Patronizing Me? Understanding And Identifying The Implications For Benevolent Racism On Inclusion In The Workplace, Nisha Quraishi

Theses and Dissertations

This study examined the construct of benevolent racism. The purpose of this study was to address the following areas. First, to distinguish benevolent racism in the racism, benevolence, and sexism literature. Second, to examine how minorities experienced benevolent racism in the workplace. Third, to use that data to conduct a quantitative analysis that examined the impact of benevolent racism on work group identification and inclusion. To do so, two studies were conducted. The first study was a qualitative study that conducted interviews which examined how benevolent racism impacted minorities in the workplace. The second study was a quantitative study that …

Punishing Pregnancy Loss: Miscarriage Criminalization As An Embodied Carceral Practice, Robin O'Hanlon

Publications and Research

The persecution of Brittany Watts, a 34-year-old Black Ohio woman arrested in September 2023 after experiencing a miscarriage, is emblematic of pregnancy loss criminalization as an embodied carceral practice. In the United States, the expansion of “fetal personhood” laws, coupled with a desire to punish imperfect pregnancies, have coalesced to produce policies and practices which actively police and criminalize pregnancy loss. The carceral regime of fetal personhood is further reinforced by medical paternalism and surveillance. I argue that the policies and practices which police pregnancy loss are rooted in a series of carceral logics, which are in turn reinforced by …

Efficient Fully Bayesian Approach To Brain Activity Mapping With Complex-Valued Fmri Data, Zhengxin Wang, Daniel B. Rowe, Xinyi Li, D. Andrew Brown

Mathematical and Statistical Science Faculty Research and Publications

Functional magnetic resonance imaging (fMRI) enables indirect detection of brain activity changes via the blood-oxygen-level-dependent (BOLD) signal. Conventional analysis methods mainly rely on the real-valued magnitude of these signals. In contrast, research suggests that analyzing both real and imaginary components of the complex-valued fMRI (cv-fMRI) signal provides a more holistic approach that can increase power to detect neuronal activation. We propose a fully Bayesian model for brain activity mapping with cv-fMRI data. Our model accommodates temporal and spatial dynamics. Additionally, we propose a computationally efficient sampling algorithm, which enhances processing speed through image partitioning. Our approach is shown to be …

Improved Management Strategies For Botrytis Fruit Rot, Macrophomina Root Rot, And Verticillium Wilt In California Strawberry Production, Joseph A. Ramirez

Master's Theses

Strawberry production in California is limited by plant diseases such as Macrophomina root rot (MRR) caused by Macrophomina phaseolina, Verticillium wilt (VW) caused by Verticillium dahliae, and Botrytis fruit rot (BFR) caused by Botrytis cinerea. Current disease management strategies are compromised due to fumigant regulations or ineffective disease management practices. This thesis investigated methods to potentially improve the management of these three diseases. Two host plant resistance evaluations for MRR and VW were conducted for the 2022-2023 and 2023-2024 growing seasons. Sixty-three strawberry genotypes were screened in both years. All plants were exposed to the pathogen via artificial …

More Concerns About Atmospheric Methane Removal Efforts, Joshua Luczak

Research Collection College of Integrative Studies

The National Oceanic and Atmospheric Administration reported record-high global atmospheric carbon dioxide levels (419.3 ppm) in 2023, alongside atmospheric methane levels (1922.6 ppb) now over 160% above pre-industrial levels. The World Meteorological Organization predicts 2024 could surpass 2023 as the warmest year on record, with more frequent and severe extreme weather events. Meeting the Paris Climate goal of limiting global warming to well below 2°C—or ideally 1.5°C—above pre-industrial levels is becoming increasingly difficult. To address these challenges, efforts are expanding beyond reducing greenhouse gas emissions. While carbon dioxide removal (CDR) technologies have been the focus, attention is now turning to …

Buyer Perceptions Of Single- And Mixed-Gender Dyadic Sales Teams: Should I Have A Wingman Or A Wingwoman?, Eric Kushins, Melissa Clark

Journal of Applied Marketing Theory

Prior research suggests that the gender composition of sales teams may impact team performance. However, research at the intersection of sales, gender, and teams is limited. In this study, we collect survey data from hypothetical buyers who viewed one of six sales pitch videos, including solo salesmen and saleswomen, single-gender dyadic sales teams, and mixed-gender sales teams. The hypothetical buyers were asked to rate each of the salespeople on important traits that have proven to lead to successful sales interactions, including customer orientation, understanding, trustworthiness, awareness of others’ feelings, competence, knowledge, and if the hypothetical buyer would take advice from …

Variation In Stress Response Regulation Across Natural Isolates Of Saccharomyces Cerevisiae, Crystal Christine Crook

Graduate Theses and Dissertations

The stress response of Saccharomyces cerevisiae is a valuable area of study for understanding cellular adaptation mechanisms that govern survival in fluctuating environments. This dissertation investigates stress response regulation in yeast through the roles of Hog1 mitogen-activated protein kinase (MAPK) and the post-transcriptional regulator Mkt1, a key post-transcriptional regulator, in diverse wild yeast isolates. Hog1 is traditionally known for its central role in osmotic stress regulation in the high-osmolarity glycerol (HOG) pathway. This study expands the functional scope of Hog1 to include oxidative and ethanol-induced stress responses.

In parallel, we investigate post-transcriptional regulation of stress responses by the Mkt1 interactome, …

Elucidating The Mechanisms Of Collagenase H From Hathewaya (Clostridium) Histolytica In Collagen Degradation And Developing A Cross-Linked Platform For Efficient Capture And Release Of Adeno-Associated Virus Serotype 2 (Aav2), Adjoa Otubea Bonsu

Graduate Theses and Dissertations

In biological systems, Hathewaya (Clostridium) histolytica secretes collagenases that degrade structural collagen in host tissues and cause tissue necrosis. Despite their detrimental effects, these enzymes have significant therapeutic potential by effectively digesting various collagen subtypes to treat connective tissue disorders. The bacterium produces two homologous collagenases, ColG and ColH, with a catalytic module crucial for hydrolyzing collagen and binding domains, including collagen-binding domains (CBDs) and polycystic kidney disease-like domains (PKDs), for substrate interaction.

Although the crystal structure of the catalytic module of collagenase G (ColG) has been determined, shedding light on its chew-and-digest mechanism, the structure of collagenase H (ColH) …

Ai V. Mqd: Navigating New Regulatory Challenges In The Life Sciences, Hayden W. Smith

William & Mary Law Review

This Note proceeds in four parts. Part I introduces AI as an emerging technology in the life sciences, the FDA’s inherited regulatory regime, and challenges under the MQD [major questions doctrine]. Part II explores how hypervigilant regulation obstructs innovation and exposes agencies to judicial challenges. Part III offers solutions to safely and effectively navigate the major questions landscape within a framework that fosters AI innovation. This Note proposes a solution that conceptually balances growth in the life sciences with the statutory elements required for a safe and effective regulatory scheme.

This abstract has been taken from the author's introduction.

The Ties That Bind: Co-Twin Influences On Psychopathology In A Sample Of Twins, Anjoli E. R. Cero

Doctoral Dissertations and Projects

Research has begun to better describe twinship and twin identity development, the potential advantages and disadvantages of twinship, and factors related to twins’ shared psychological symptomatology. Research also supports the concept of emotional contagion, in which one person’s emotional state can be transmitted to another person. This phenomenon may be more common among twins due to factors such as closeness, dependence, and general similarity. The current study proposed a series of regression models to investigate how predictive pathways toward anxiety among a sample of twins could be impacted by the psychological traits and functioning of their co-twins. The current sample …

Understand The Power Of Resilient Adult Survivors Of Childhood Neglect, Sheena Smith

Doctoral Dissertations and Projects

The purpose of this qualitative phenomenological study was to explore the lived experiences of adults from New York (NY) and New Jersey (NJ) who have overcome adverse childhood experiences and describe their experiences in becoming resilient. Adverse childhood experiences have been linked to various health disparities, including depression, anxiety, substance misuse, and physical health concerns. However, limited research focuses specifically on resilience among survivors of childhood neglect and adversity. The study investigated the subjective experiences of adults residing in New York and New Jersey who have experienced high levels of ACEs, including neglect and abandonment. The study answered the following …

The Lived Experiences Of Black Christian Women With Having Absent Fathers: A Phenomenological Study Of Identity, Self-Esteem, And The Christian Faith, Catherine L. Newsome

Doctoral Dissertations and Projects

This qualitative phenomenological study examined the lived experiences of 10 Black Christian women with absent fathers during childhood and adolescence. Using convenience sampling, these women were recruited through Facebook, TikTok, and email recruitment at Liberty University. The study’s criteria for participation were: (1) Black Christian women 18 years of age or older, (2) raised in a fatherless home during childhood and adolescence, and (3) raised with no stepfather in the home during childhood and adolescence. The study's findings revealed that the participants experienced a void due to the absence of a father figure, the lack of a father’s provision, and …

A Phenomenological Study Of Guam Public-School Elementary Teachers’ Transitional And Mental Health Experiences During The Covid-19 Global Pandemic In 2020, Jessica Fausto Villaverde

Doctoral Dissertations and Projects

The purpose of this phenomenological study is to understand the lived experiences that may have impacted the mental health of public-school elementary teachers in Guam as they entered an unfamiliar period of teaching during the 2020 coronavirus (COVID-19) global pandemic. The theories guiding this study are Nancy Schlossberg’s transition theory and social constructivism which was originated by Lev Vygotsky and John Piaget. The philosophical assumption of this study is ontology which assisted in reporting the different realities of its participants. Schlossberg’s transition theory guided this study for understanding teacher transitions and changes, and the social constructivism worldview captured various mental …

Engaging Church Teams To Support Youths Aging Out Of Care, John Alan Honeycutt

Doctoral Dissertations and Projects

Emerging Adults (EAs) in out-of-home care (OHC) face significant challenges when aging out of the foster care system, including histories of traumatic experiences. Approximately 30,000 exit Child Protective Services (CPS) annually, with 4,000 becoming instantly homeless. This study used a facilitated grounded theory approach, engaging 14 participants representing two churches. The aim was to identify the topmost factors for gaining interest, commitment, and action from a Volunteer Church Team Volunteer Team to assist one EA in their transition to independent living (IL). Primarily, non-Hispanic White and Native American Indian participants aged 35–65 contributed insights during three hours of brainstorming and …

A Qualitative Phenomenological Exploration Of The Experience Menopausal African American Women Have With Their Health Care Providers And Accessing Treatment For Menopausal Symptoms, Michelle Way-Houser

Doctoral Dissertations and Projects

This phenomenological qualitative case study delved into the underexplored area of menopausal research as it pertains to African American women. This study aimed to identify the existing gap in literature, through examining the factors contributing to the disparity, and proposed potential solutions. By conducting a thorough review of the available evidence, this study sheds light on the unique challenges faced by African American women during menopause and advocates for more inclusive and diverse research approaches in this field. Semi-structured interviews were conducted with ten participants and thematic analysis was used to identify key themes related to symptom management, healthcare access, …

Understanding Change Following Psychedelic-Assisted Therapy: A Phenomenological Study, Melina K. Bricker

Doctoral Dissertations and Projects

The field of psychedelic-assisted therapy (PAT) is growing and broadening, with significant implications for treatment-resistant behavioral health disorders for which traditional therapies have limited impact. Determining the efficacy of such treatments with populations seeking relief from trauma-related chronic behaviors must include an understanding of trauma’s complex impact on regulatory functioning, the perceptual and functional changes experienced as a result of PAT, and implications for future research in addressing trauma-related treatment resistance. To understand the phenomenon of change, questions of motivation, experience, and outcomes in this treatment type must be understood. In this qualitative phenomenological study, 10 patients at a ketamine-assisted …

A Phenomenological Study Exploring Teacher-Student Relationships For Students With Moderate To Severe Disabilities, Karen N. Holland

Doctoral Dissertations and Projects

The purpose of this qualitative, transcendental, phenomenological study was to understand the experiences of general education and self-contained classroom teachers building relationships with students who have moderate to severe disabilities in rural Maryland. Teachers were selected from elementary school settings in the rural part of Maryland’s eastern shore. The theory that guided this study was Bowlby’s attachment theory. Vygotsky focused on language development and how it affects social interactions between others while Bowlby’s theory explored a child’s attachment to close adults. This study explored teacher-student relationships as it relates to elementary-age students on Maryland’s eastern shore. The methodology for this …

A Qualitative Study Of The Lived Experiences Of Individuals Whose Parents Or Grandparents Had/Have Substance Abuse Issues In The Midwest, Brandie Lee Gaudet

Doctoral Dissertations and Projects

This study focused on the lived experiences of individuals being raised by individuals who had/have substance abuse issues in the Midwest region of the United States of America. A closer look at this topic offered new insight into treatment and resources for adult children raised by parents/grandparents (collectively “parents”) with substance abuse issues (“SAI”) and how the parents’ substance abuse issues compromised the behavioral health outcomes of the adult children and affected the parents’ parenting outcomes. Understanding the role of family systems theory in adaptive and maladaptive behaviors is essential when formulating a plan to help individuals’ negatively impacted by …

From Recovery To Restored: A Thesis Action Project Developing Addiction Restored Groups Transitioning Individuals Out Of A Recovery Cycle And Into A Church Small Group, Scott Kingsley Schneiderman

Doctoral Dissertations and Projects

People at New Beginnings Church (NBC) who suffer from addiction currently do not have an intentional path into a life-giving Core Group without the need for ongoing support groups. This DMIN action research project aims to design, implement, and evaluate Restored, at NBC, an eight-week addiction recovery process. Restored utilizes individual self-evaluation through Scripture, collective discussion, and tailored sin-cycle diagrams. The innovation in this project focuses on the transitional phase, unlike other addiction recovery programs, and the integration of Christians into a church small group. This DMIN action research project will enable Christians struggling with addiction to identify their sin, …

A Quantitative Study Teaching De-Escalation With Virtual Reality Simulation To Associate Nursing Students Measuring Effectiveness, Learning, And Confidence, Michele Rene Marbet

Doctoral Dissertations and Projects

Workplace violence against nurses is a critical global public health issue, as one in four nurses reportedly experience physical assault by patients. This quantitative quasi-experimental, pretest-posttest study aimed to assess the effectiveness of virtual reality simulation as a teaching strategy for de-escalation education in associate nursing students’ (N = 29) perception in learning and confidence. The study utilized the Simulation Effectiveness Tool-Modified (SET-M) instrument to measure outcomes guided by Kolb's experiential learning theory and the NLN Jeffries simulation theory. The learning variable demonstrated significant improvement as confirmed by paired t-test analysis (p < .01). For the effectiveness and confidence variables, which violated assumptions of normality, a comprehensive statistical approach was implemented. Analysis included parametric testing via paired t-tests, supplemented by nonparametric methods (Wilcoxon Signed-ranks test and Sign test) to ensure statistical rigor. The cumulative results across all three statistical tests consistently demonstrated significant improvements (p < .001). Effect size calculations revealed medium to large significance for all variables measured by Cohen’s d. The conclusive findings of this research included a rejection of all three null hypotheses, which suggests that virtual reality simulation is an effective teaching strategy for developing de-escalation skills, enhancing both learning and confidence among associate nursing students. Future studies are needed to further validate and expand upon the findings of the current research, potentially leading to more widespread adoption of virtual reality simulation in nursing education for teaching de-escalation.

The Church’S Response Post Grey Divorce: A Phenomenological Study Of Evangelical Men Experiencing Grey Divorce And The Implications For Faith And Post-Divorce Adjustment, Randall Rush

Doctoral Dissertations and Projects

In 1990 one out of every ten divorces involved an individual over the age of fifty. By 2010, that number had climbed to one out of every four divorces. As of 2019, one in three people getting divorced in the United States was aged fifty or older. Termed by researchers as “grey divorce,” this phenomenon is expected to grow as demographic shifts reveal an increasing older adult population with longer life expectancies. These later-life divorces have psychosocial and economic negative consequences on the individual. Divorcing later-life men and women share a number of these negative consequences, but there is gender-based …