Digital Commons Network^™

Intersectionality In Tennis (Wta) Sports Media, Emily Stoner

Honors Program: Senior Projects (Public)

This “applied knowledge” project investigates and observes intersectionality in tennis sports media via the women’s professional tour (WTA). Intersectionality in Tennis (WTA) Sports Media analyzes how intersectionality as a concept and social justice lens impacts and is impacted by media. By considering intersectional identities and their implications in sports media narratives and coverage, this piece displays some telling patterns and perpetuations.

At the professional level, tennis certainly does not garner the most profit or attention, but it provides a unique world of intersecting identities, cultures, geography and tradition. This work aims to shed light on the state of sports media, …

The Digital Glow: Effects Of Blue Light On Children And The Development Of A Children's Book To Raise Awareness On Eye Health, Mallory Easland

Honors Program: Senior Projects (Public)

As digital screen use continues to rise among children, concerns about the effects of blue light on eye health, sleep patterns, and overall well-being have grown. Research suggests that prolonged exposure to blue light can contribute to digital eye strain, disrupt circadian rhythms, and impact cognitive development. This thesis explores scientific literature on the biological effects of blue light, specifically on children, examining both the risks associated with excessive exposure and strategies for mitigating its impact.

In addition to conducting a comprehensive literature review, this project applies research findings to the creation of an educational children’s book aimed at promoting …

Gut Feeling: A Holistic Digital Tool For Managing Irritable Bowel Syndrome And Food Sensitivities, Hailey M. Olberding

Honors Program: Senior Projects (Public)

Irritable Bowel Syndrome (IBS) and food intolerances are increasingly prevalent chronic gastrointestinal (GI) conditions that significantly impair quality of life. While the low-Fermentable Oligosaccharides, Disaccharides, Monosaccharides and Polyols (FODMAP) diet has demonstrated success in symptom management, current digital tools fail to offer integrated, user-friendly platforms that holistically track contributing health factors. Gut Feeling is a mobile app (Appendix 1) and complementary website (Appendix 2) developed to fill this gap. By incorporating symptom logging, food intake tracking and complementary database, stress and sleep monitoring, and trend analysis, this platform empowers users to better understand their bodies and manage GI symptoms proactively. …

Entre La Reforma Y La Realidad: Salud Y Derechos Humanos En Costa Rica, Cuba Y Uruguay, Alyssa M. Nielsen

Honors Program: Senior Projects (Public)

Latinoamérica tiene varios sistemas para el cuidado de la salud y el acceso es uno de la lista de los derechos humanos. Esta región es la más desigual del mundo y se han logrado grandes avances, pero no se han distribuido equitativamente. Los sistemas de Cuba, Costa Rica y Uruguay son bien desarrollados, pero funcionan diferente. Costa Rica y Uruguay tienen opciones públicas y privadas y tienen maneras como la Mutualista en Uruguay y la Caja en Costa Rica para cubrir las visitas y servicios necesarios. Cuba es el sistema más controversial y tiene gran influencia de Castro, de donde …

Gene Promoters Are Genomically Encoded To Facilitate Histone Exchange/Incorporation, Blaine Bartholomew

Faculty, Staff and Student Publications

Gene promoters are intrinsically hardwired not only to facilitate transcription preinitiation complex formation but also the release of paused RNA polymerase by H2A.Z. A new PLOS Biology study reveals poly (dA) tracts at promoters positively stimulate H2A.Z incorporation by the SWR complex in yeast.

Igtp: Learning Interpretable Cellular Embedding For Inferring Biological Mechanisms Underlying Single-Cell Transcriptomics, Kang-Lin Hsieh, Kai Zhang, Yan Chu, Lishan Yu, Xiaoyang Li, Nuo Hu, Isha Kawosa, Patrick G Pilié, Pratip K Bhattacharya, Degui Zhi, Xiaoqian Jiang, Zhongming Zhao, Yulin Dai

Faculty, Staff and Student Publications

Deep-learning models like Variational AutoEncoder have enabled low dimensional cellular embedding representation for large-scale single-cell transcriptomes and shown great flexibility in downstream tasks. However, biologically meaningful latent space is usually missing if no specific structure is designed. Here, we engineered a novel interpretable generative transcriptional program (iGTP) framework that could model the importance of transcriptional program (TP) space and protein-protein interactions (PPI) between different biological states. We demonstrated the performance of iGTP in a diverse biological context using gene ontology, canonical pathway, and different PPI curation. iGTP not only elucidated the ground truth of cellular responses but also surpassed other …

Finding Bert Errors By Clustering Activation Vectors, William B. Andreopoulos, Dominic Lopez, Carlos Rojas, Vedashree P. Bhandare

Faculty Research, Scholarly, and Creative Activity

The non-linear nature of deep neural networks makes it difficult to interpret the reason behind their output, thus reducing verifiability of the system where these models are applied. Understanding the patterns between activation vectors and predictions could give insight as to erroneous classifications and how to identify them. This paper explains a systematic approach to identifying the clusters with the most misclassifications or false label annotations. For this research, we extracted the activation vectors from a deep learning model, DNABERT, and visualized them using t-SNE to decode the reason behind the results that are produced. We applied K-means in a …

Whole Grains And Ancient Wheats: Knowledge, Attitudes, Consumption Behavior And Sensory Liking Of Breads Among Participants In The United States, Caroline Morey, Jamie Kubota, Sulekha Anand, Xi Feng

Faculty Research, Scholarly, and Creative Activity

Consumer interest in ancient grains has been growing in the United States over the last 10 years. Whole grain ancient wheats incorporation into breads may be a way to increase whole grain consumption among Americans, but there is no research evaluating sensory liking of whole ancient wheat breads among American consumers. The objective of this study was to explore the sensory liking of whole ancient wheat bread types and to explore factors that may be associated with sensory preferences. The study recruited 104 untrained participants of diverse ethnicities affiliated with San José State University (San José, CA 95192, USA). Participants …

Histomorphology Of Sharp-Force Trauma On Partially Cremated Remains, Horaceena Findley

Student Theses

Identifying sharp-force trauma in thermally altered human remains presents a significant challenge in forensic investigations, particularly in arson-related deaths where traditional autopsy techniques may be limited. This study explores the efficacy of histological methods in detecting sharp-force trauma on partially cremated remains using pig ribs (Sus scrofa) as a human analog. Simulated tool marks from a knife, screwdriver, and flathead screwdriver were inflicted at varying angles (60° and 90°) and subsequently exposed to high temperatures in an open fire setting. Histological analyses, including Hematoxylin and Eosin Y (H&E) and Gomori’s Prussian Blue staining, were employed to assess tissue structure and …

Passive Dna Transfer To Fidget Blasters Stored In Cars, Rachel Chan

Student Theses

This study evaluated the amount of detectable DNA accumulated on clean plastic fidget blasters, used to simulate firearms, over a seven-day storage period inside a vehicle. Twelve volunteers were asked to place one fidget blaster each in the glove box and underneath the driver’s seat from behind. After the seven-day period, the volunteers returned the fidget blasters, donated a reference sample, and filled out a questionnaire. The grip, slide, and trigger were swabbed for DNA and extracted. The extracts were quantified to determine the concentration of human DNA, and if the samples met the PCR threshold to be genotyped. The …

Graded Response Of The Pulmonary Circulation To Progressive Pulmonary Embolism In Sheep: From Compensation To Lethal Right Heart Failure, Cheng Zhou, Yuman Li, Kyle Polley, Cherry Ballard-Croft, Masashi Kawabori, Keshava Rajagopal, Joseph B. Zwischenberger, Dongfang Wang

Department of Surgery Faculty Papers

Background: Severe pulmonary embolism (PE) with right heart failure (RHF) has high mortality. To more fully understand PE progression, we evaluated the right ventricle (RV) and pulmonary circulation response to graded PE in an acute PE sheep model.

Methods: Polydextran beads were intravenously administered every 15 minutes in 250 mg doses to adult female sheep (n = 8) until death. Concurrent pulmonary artery (PA) blood flow/pressure was measured. RV pressure-volume (P-V) loops were generated with a conductance catheter. Pulmonary vascular resistance was used to stage PE severity into mild, moderate, and severe groups.

Results: All sheep developed graded RHF. For …

Melanaphis Sacchari/Sorghi Complex: Current Status, Challenges And Integrated Strategies For Managing The Invasive Sap-Feeding Insect Pest Of Sorghum, Alejandro Vasquez, Joseph Belsky, Neetu Khanal, Heena Puri, Devi Balakrishnan, Neelendra K. Joshi, Joe Louis, Glenn Studebaker, Rupesh Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Melanaphis sacchari (Zehntner;Hemiptera: Aphididae), sugarcane aphid (SCA), is an invasive phloem-feeder found worldwide with a wide host range of economically important plants including sorghum and sugarcane. Given its high reproductive capacity and ability to rapidly spread over long distances, SCA presents challenges for effective control, leading to substantial economic losses. Recent studies have identified two multiloci SCA genotypes specialized in feeding on sugarcane (MLL-D) and sorghum (MLL-F) in the USA, which raises concerns as the USA is the second largest sorghum-producing country. This has encouraged research towards identifying these two biotypes where some research has stated them as two species; …

Investigating The Effects Of Hepatocyte Growth Factor On Primary Myoblast Proliferation, Grace Heller

Biomedical Engineering

Peripheral artery occlusive disease (PAOD) is a progressive vascular condition that affects over 200 million people worldwide and approximately 8 million in the United States. It is characterized by atherosclerotic narrowing of peripheral arteries, leading to reduced blood flow, ischemia, and, in severe cases, tissue loss and limb amputation. Although surgical revascularization is the gold standard, many patients—particularly those with comorbidities like diabetes— continue to experience poor outcomes, emphasizing the need for alternative therapies. One promising strategy is to enhance collateral arteriogenesis, the remodeling and enlargement of collateral arterioles that naturally bypass occluded vessels. Satellite cells (SCs), which give rise …

Assessment Of Energy Efficiency Measures Recommended For Industrial Facilities: Environmental, Economic, And Life Cycle Perspectives, Dariush Jafari

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

This study assesses the economic, environmental, and life cycle impacts of more than 800 industrial Energy Efficiency Measures (EEMs) identified in manufacturing facilities across Nebraska. These EEMs are notable for their rapid implementation and minimal disruption to the facility’s building structure or core processes.

The research evaluates the financial viability of EEMs by analyzing utility savings, implementation costs, and payback periods. It also examines their cost-effectiveness in reducing greenhouse gas (GHG) emissions, including calculations of net cost and cost of mitigated CO₂-eq.

Key findings indicate that adding insulation to hot pipes and buildings, replacing personal fans with High-Volume, Low-Speed (HVLS) …

Addressing Structural Violence In Eastern Nebraska Through The Assessment Of Mortality Profiles, Victoria M. Pate

Department of Anthropology: Theses and Student Research

Humanitarian forensics has recently expanded to include the examination of forensic casework demographics within the medicolegal community. Additionally, it is proposed that research and data collection should look beyond the individual case report to address public health, crime vulnerabilities, and marginalization at local and national levels to advocate for policy changes protecting against institutionalized violence. With the utilization of quantitative and qualitative methods, 294 case reports provided by Nebraska police departments, between the years of 2014-2024, were analyzed to conceptualize mortality vulnerabilities and the ways in which forensic anthropologists can aid in community action. Pearson’s Chi-Square and one-way Analysis of …

Wired For Degradation: How Post-Translational Control Of Inducible Camp Early Repressor Protein Influences Melanoma Fate, Melissa Rose Spigelman

Theses, Dissertations and Culminating Projects

Melanoma is a highly aggressive form of skin cancer frequently driven by mutations in the BRAF gene, particularly the BRAFⱽ⁶⁰⁰ᴱ variant, which leads to sustained activation of the MAPK signaling cascade. Recent studies implicate the cyclic AMP (cAMP) signaling axis in melanoma progression and therapeutic resistance, highlighting the role of the Inducible cAMP Early Repressor (ICER), a transcriptional antagonist of CREB and CREM. ICER is downregulated during melanomagenesis and subject to post-translational regulation via phosphorylation and ubiquitin-mediated degradation. To investigate ICER’s functional relevance in vivo, e utilized a zebrafish melanoma model harboring brafⱽ⁶⁰⁰ᴱ, tp53−/−, and mitf−/−mutations, in which mitf expression …

Host Genetics Role In Viral Disease Susceptibility, Christian Willis Eaton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Viruses and their hosts exist in an evolutionary arms race, with evolutionary promotion of host genetic-based defense mechanisms and evolution of virus genetic-based mechanisms to overcome host defenses. Viruses are capable of much more rapid evolution and adaptation than their hosts, often leading to the use of host cellular mechanisms to promote viral infection and replication. In this study, we describe the generation of a detection assay for atypical porcine pestivirus (APPV), designed to target the viral 5’ UTR, a region of the viral genome which sees less mutations than the protein coding regions. This assay was generated to be …

Gonadotropin-Releasing Hormone Ii And Its Receptor: Novel Regulators Of Semen Quality And Follicular Function In Swine, Caitlin Elizabeth Ross

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Pigs are the only mammalian livestock species encoding functional proteins for the second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II). In the first study, GnRHR-II localized to developing germ cells and the connecting piece of ejaculated spermatozoa. Concurrently, GnRH-II was most abundantly produced in the testis compared with other reproductive tract organs and was present within seminal plasma. Moreover, treatment of extended semen with GnRHR-II analogues altered sperm motility, morphology, and kinematics. For remaining studies, we utilized a swine line with ubiquitous knockdown of GnRHR-II (GnRHR-II KD) developed in our laboratory. The second study demonstrated that circulating GnRH-II …

Interferon Regulatory Factor 3 In Interferon Gamma Receptor Signaling And Cancer Immunology, Zachary P. Guinn

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Every year millions of people die from cancer throughout the world, and tens of millions of people are diagnosed with cancer worldwide. When surgery is not an option, treatment options commonly rely on the host immune system for optimal treatment efficacy. The expression of Interferon gamma (IFN-γ) by host immune cells is often associated with favorable antitumor immune responses. How IFN-γ is optimally induced in immune cells, and how IFN-γ promotes the transcription of genes downstream of the Interferon gamma receptor (IFNGR) is incompletely understood. Understanding how to induce immune cells to express optimal IFN-γ …

Utility Of John Deere's See & Spray Ultimate In Southern U.S. Row Crops, Tristen Avent

Graduate Theses and Dissertations

Row crop producers across the southern United States are observing a boom in technological advancement to improve operational efficiency. Additionally, environmental concerns and reduced profit margins drive the adoption of targeted pesticide application technologies like the John Deere See & Spray. This technology is relatively new, introduced in 2022, and producers need more insight into the shortcomings, potential herbicide savings, and the economic benefits of adoption. Since 2021, research has been conducted in Keiser, AR, with additional collaborative projects in Mississippi, North Carolina, Indiana, and Illinois. Five different objectives were addressed in this research: 1) to determine the impact of …

Toward Whole-Person Fit Assessment: Integrating Interests, Values, Skills, Knowledge, And Personality Using The Occupational Information Network (O*Net), Zihan Liu, Kevin A. Hoff, Chu Chu, Frederick L. Oswald, James Rounds

Research Collection Lee Kong Chian School Of Business

Measuring person–occupation fit serves many important purposes, from helping young people explore majors and careers to helping jobseekers assess fit with available jobs. However, most existing fit measures are limited in that they focus on single individual difference domains without considering how fit may differ across multiple domains. For example, a jobseeker might be highly interested in a job, yet not possess the requisite skills or knowledge to perform the job well. The current research addresses this issue by evaluating an integrative set of person–occupation fit assessments that measure 88 fit dimensions across five domains: vocational interests, work values, knowledge, …

Influence Of Colony And Hive Attributes, Landscape, And Seasonality On Occurrence Of Large Hive Beetles (Coleoptera: Scarabaeidae) In Taita Taveta County, Kenya, Mbatha B. Wambua, Ayuka T. Fombong, Kiatoko Nkoba, Gladys K. Onyambu, Helen Lydiah Kutima, Sevgan Subramanian, Beatrice T. Nganso

All Peer-Reviewed Publications

Recently, an unexpectedly large number of large hive beetles, Oplostomus haroldi (Witte), was observed in Taita Taveta County (TTC), Kenya, suggesting a potential threat to the apicultural industry. This study investigated the influence of colony and hive attributes, landscape characteristics, and seasonality on the occurrence of O. haroldi within beehives in TTC. Field surveys assessed beetle populations alongside colony strength parameters, environmental variables, and hive types using standard protocols. Our findings revealed significant positive correlations between beetle occurrence and colony phenotypes, such as capped brood, pollen, and honey quantities. Higher beetle populations were also observed in areas with tree cover …

Harnessing Edna Metabarcoding To Monitor Species Diversity In Restoration Sites: Insights From Laikipia, Kenya, Cornelius Okello, Nina Wambiji, Susana W. Kihia, Nehemiah Ongeso, Samuel Mwakisha Mwamburi, Anthony Wandera, Marie Estelle Demory, Margaret Awuor Owuor

All Peer-Reviewed Publications

Restoration of degraded arid and semi-arid lands (ASALs) dryland ecosystems gained traction globally with the enactment of the Bonn Challenge and UN Decade of Restoration. This has been domesticated in Kenya's context by The Forest and Landscape Restoration Implementation Plan (FOLAREP). However, effectively monitoring restored ecosystems is crucial but challenging because of data gaps and technical hurdles, highlighting the need for innovative approaches to assess and restore biodiversity. This study aimed to pilot soil environmental DNA (eDNA) metabarcoding to detect species compositions rapidly, evaluate population structures and abundances, and characterize taxonomic groups. The results from this study serve as a …

Long-Term Cropping System And Manure Effects On Soil Health Parameters And Associated Soil-Borne Pathogens, Joyce Mutai, Beth Medvecky, Steven J. Vanek, John Ojiem, Peter Bolo, Job Kihara, Steven J. Fonte

All Peer-Reviewed Publications

Soil-borne pathogens reduce the performance of key food crops in sub-Saharan Africa. Diversified cropping and nutrient management can enhance soil and plant health, limiting pathogen damage. To examine how management and soil health changes influence soil-borne pathogens, we leveraged an 18-year field trial in western Kenya, evaluating cropping systems typical of smallholder farms. We considered three cropping systems and two organic matter management strategies: continuous maize monocrop (M–M), Tephrosia in rotation with maize (T–M), maize intercropped with soybean (M–S), application or not of farmyard manure, and retention or removal of crop residues. We assessed soil physical and chemical properties and …

Glutamate Gating Of Ampa-Subtype Iglurs At Physiological Temperatures, Anish Kumar Mondal, Elisa Carrillo, Vasanthi Jayaraman, Edward C Twomey

Faculty, Staff and Student Publications

Ionotropic glutamate receptors (iGluRs) are tetrameric ligand-gated ion channels that mediate most excitatory neurotransmission1. iGluRs are gated by glutamate, where on glutamate binding, they open their ion channels to enable cation influx into postsynaptic neurons, initiating signal transduction1,2. The structural mechanics of how glutamate gating occurs in full-length iGluRs is not well understood. Here, using the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype iGluR (AMPAR), we identify the glutamate-gating mechanism. AMPAR activation by glutamate is augmented at physiological temperatures. By preparing AMPARs for cryogenic-electron microscopy at these temperatures, we captured the glutamate-gating mechanism. Activation by glutamate initiates ion channel opening that involves all ion …

Crispr-Cas9 Integration Of Salmon Elovl2 In Zebrafish Enhances Lc-Pufa Biosynthesis And Growth Across Diets, Waleed Sami J. Al-Bachry

Graduate Theses and Dissertations

Fish are a rich source of EPA and DHA, which are well-known long-chain polyunsaturated fatty acids (LC-PUFAs). The sustainable production of these essential fatty acids poses significant challenges, mainly due to our dependence on marine fish and certain nuts. CRISPR-Cas system was utilized to insert the elongation of long-chain fatty acids-like2 (Elovl2) gene from Salmo salar into Danio rerio. Elovl2 was introduced at 10-15-minute post-fertilization, and successful integration confirmed by PCR genotyping, sequencing and expression of EGFP. Approximately 67% of injected embryos successfully exhibited EGFP and sequence results verified the gene integration of Salmo salar Elovl2 at the first start …

Nonparametric Methods For Bayesian Community Detection In Complex Networks, Kedran Young

Graduate Theses and Dissertations

Network analysis is becoming an increasingly popular interdisciplinary area of study, with emerging interest in fields like sociology, biology, economics, and ecology. Within the niche of network analysis, capturing the community structure of a network is one important achievement that many statisticians have been working toward over recent decades. The most popular modeling technique for latent community detection is the Stochastic Block Model (SBM), which falls into the category of latent variable models and will serve as the baseline model throughout this thesis. SBM is widely regarded as the most effective community detection method as it detects latent community membership …

Gender, Politics, And Power: How Women Presidential Candidates Are Framed By News Narratives, Victoria Hernandez

Graduate Theses and Dissertations

The aim of this study was to look at the coverage of the presidential campaigns of Hillary Clinton in 2016 and Kamala Harris in 2024 during the Democratic National Convention, and analyze the differences in regard to gender, race, and political bias. The examination of these news narratives helps explore a question of whether journalists report with bias when covering politics. The researcher analyzed 11 published pieces from five mainstream media outlets. Findings of the study show that bias is unavoidable even when a journalist strives for objectivity which does not exist in newsrooms across the political spectrum. The findings …

Investigating Evolution Through The Lens Of Ai-Driven Protein Exploration And Phylogenetic Modeling, Mataya Duncan

Graduate Theses and Dissertations

Recent advances in computational biology, artificial intelligence, and sequencing technologies have enabled new perspectives for addressing longstanding questions in evolutionary biology. Increased computational power has made large-scale simulations feasible for investigating diverse aspects of evolutionary and phylogenetic modeling. Similarly, deep learning algorithms now allow for the prediction of reasonably accurate tertiary protein structures, unlocking the potential for investigating diversity and evolution of protein structure across a broad range of organisms. This thesis presents two studies that target different aspects of evolutionary inference from unique perspectives. Yet, the share an overarching goal of presenting new perspectives on the evolutionary basis of …

Experiences Of Black Senior Housing Officers At Predominantly White Institutions In The South, Christian M. Robinson

Graduate Theses and Dissertations

This study examines the lived experiences of Black Senior Housing Officers (SHOs) at predominantly white institutions (PWIs) in the Southern United States. This narrative inquiry study interviewed nine Black SHOs who worked at Southern PWIs. The findings of this study are that participants emphasized that racism remains a significant and present concern at their institutions as informed by Critical Race Theory (CRT). One participant stressed the importance of Black professionals fully understanding the historical context of the South when working in higher education, noting that this knowledge was critical for survival and success. Many participants also described how effective mentorship …