Digital Commons Network^™

Assessing The Relationship Between The Incidence Of Campylobacteriosis And Ecological Risk Factors In Tennessee, Emmanuel Angmorteh Mensah

Electronic Theses and Dissertations

Background: Campylobacteriosis although is the leading causes of bacterial diarrheal illnesses, have not gained prominent awareness and there are gaps in the understanding of transmission routes for sporadic, non-outbreak cases and their risk factors at the communal level to humans. This study, therefore, aimed to identify the campylobacteriosis risk factors and risk perception from the existing literature in the United States, determine the incidence and ecological risk factors for campylobacteriosis zip code level in Tennessee, and to perform spatiotemporal analysis of campylobacteriosis incidence cases in Tennessee.

Method: To achieve the first aim, a scoping review was conducted to synthesize …

The Moderating Role Of Social Support For Lgbtq People In South Central Appalachia: Present Yet Unimpactful?, Paige Henry

Electronic Theses and Dissertations

Lesbian, gay, bisexual, transgender, and queer (LGBTQ) minority stress theory states that LGBTQ people experience stressors specific to their identity that result in negative mental health outcomes. Social support has been shown to protect against these negative outcomes, but South Central Appalachia is known to be high stigma and low resource for this population. The present study examined the potential moderating impact of four sources of social support (family, friends, partners, and pets) on the relationship between minority stress experiences and psychological distress for LGBTQ people living in South Central Appalachia. Participants included 116 LGBTQ adults living in South Central …

The Forest Through The Trees: Modeling The Ecological Niche And Potential Assemblage Distribution Of Oak-Hickory Forests Across The Eastern U.S., Elijah Worley

Electronic Theses and Dissertations

Understanding how tree species’ ranges and populations may shift by the end of the 21^st Century is critical for effective conservation and forest management. Ecological niche models are widely used to predict species distributions by modeling the relationships between environmental variables and species presence. While numerous models have been developed for individual tree species across the USA, there is limited research on how tree species assemblages, such as oak-hickory forests, will respond to changes in climate over time. Maxent modeling software was used to predict potential shifts in suitable habitat for the oak-hickory forest type, based on environmental variables …

Unraveling The Unknown: Characterization Of A Novel Antimicrobial Compound Found In Klebsiella Pneumoniae, Jasmal Cruz Perez

Electronic Theses and Dissertations

Klebsiella pneumoniae, a pathogenic bacterium resistant to numerous antimicrobials, is a worldwide health issue due to its ability to cause healthcare-associated diseases such as pneumonia and urinary tract infections. This study investigated an antimicrobial compound secreted by K. pneumoniae. The compound was found to possess inhibitory activity against Enterobacteriaceae members, including other Klebsiella pneumoniae strains, Citrobacter freundii, and Enterobacter cloacae. The inhibitory activity is mediated via a secreted protein that is active across a wide range of pH and temperature conditions. Higher concentrations of the compound enhanced antimicrobial activity. The antimicrobial protein suppressed biofilm formation during both …

Exploring The Antagonistic Polymicrobial Interaction Of Alcaligenes Faecalis With Prokaryotic And Eukaryotic Microorganisms, Aly Hargrave

Electronic Theses and Dissertations

Alcaligenes faecalis, a generally nonpathogenic, Gram-negative bacterium, has been observed by our laboratory to possess antagonistic activity against antimicrobial-resistant genera Staphylococcus, Candida, and Bacillus. This study focused on exploring the inhibition mechanism of A. faecalis against the three genera, with an emphasis on S. aureus. A library of A. faecalis transposon mutants was constructed and identified fourteen possible genetic factors linked to this inhibitory activity. Characterization of this inhibition indicated that A. faecalis suppressed S. aureus planktonic and biofilm growth via a contact-dependent mechanism, with pronounced effects during biofilm attachment phase. In a Caenorhabditis elegans …

Kinetic Analysis Of Hydrolysis And Hydrothermal Conversion Of Methyl Cellulose To Dodecane, Joseph Olembo Were

Electronic Theses and Dissertations

Environmental pollution due to emission of greenhouse gases and increasing global energy demand are some of the concerns associated Analysis with the use of fossil fuels. These concerns have triggered the exploration of sustainable and efficient methods for renewable energy production. One such method is the conversion of cellulosic biomass to hydrocarbon fuels using hydrogen bronze metals. Cellulose is the most abundant biomass globally, and thus its utilization can lead to more sustainable energy production. Previous studies have reported the conversion of cellulose to hydrocarbons at high temperatures and hydrogen pressure. This work focuses on the conversion of methyl cellulose …

A Kinetic Analysis On Hydrolysis And Hydrothermal Conversion Of Cellulose To Dodecane, Joel Nyangoka Onyancha

Electronic Theses and Dissertations

Cellulose, the most abundant biomass on Earth, offers a promising pathway for alternative energy through conversion into alkane hydrocarbons. Traditional methods rely on high temperatures and high-pressure hydrogen, often derived from petroleum. In this study, we investigated the conversion of Microcrystalline Cellulose using hydrogen metal oxides (bronzes) under milder conditions. Following ASTM E3417-24 hydrolysis protocols, we employed hydrogen bronzes with toluene for product extraction. This approach enabled conversion at lower temperatures without pressurized hydrogen. Products were analyzed using Gas Chromatography Mass Spectrometer for identification and Gas Chromatography Flame Ionization Detector for quantification. Our findings revealed the formation of a possible …

Multistage Random Key Genetic Algortihm Optimization For Scheduling Flexible Flow Lines With Sequence Depenedent Setup Times, Aadithan Anbuvanan

All Theses

This thesis proposes a new variation to the Random Key Genetic Algorithm (RKGA) for scheduling optimization in flexible flow line manufacturing with sequence dependent setup times. The proposed RKGA representation decodes scheduling information independently at each stage, unlike the traditional RKGA, which is only sequenced based on the first stage, limiting flexibility. The proposed method's performance is compared to the traditional method with varying numbers of jobs and stages. It is compared regarding performance ratio and statistical significance of differences through the Wilcoxon Signed Rank Test. Results show that the proposed RKGA outperformed traditional RKGA in high complexity (8 Stage …

Impact Of Shear Flow On Marine Biofilms Using Hyperspectral Imaging, Shantanu Mahesh Kore

All Theses

Various microbial communities in marine biofilms cause biofouling on submerged surfaces, posing challenges to marine industries. Despite their ecological and economic importance, biofilms' biomechanical and biochemical responses to hydrodynamic shear stress are insufficiently understood, particularly in dynamic flow conditions. To fill this gap, our study uses an innovative fiber-optic hyperspectral imaging (HSI) system and a supercontinuum laser source to examine marine biofilms' structure, composition, and detachment behavior at different shear stress levels.

To detect spectral and spatial heterogeneity in live biofilms without labeling, we created a custom imaging pipeline that captures reflectance spectra in the 600-850 nm range, targeting microbial …

Interobserver Agreement Of Human Osteological Analysis On The Szeged, Hungary Skeletal Collection, Alleyia Bailey

All Theses

Biological anthropologists develop human skeletal biological profiles to identify skeletal remains and build demographic knowledge of past and modern peoples. Biological anthropologists estimate age-at-death, sex, stature, ancestry, and the presence of injury or pathology to develop biological profiles. While biological profiles are at the basis of the work anthropologists perform, there is a lack of research concerning the reproducibility and interobserver agreement rates of the methods used to create biological profiles. Due to the risk of variability stemming from the education, training, and experience of observers, along with variation that can arise from population affinity, examining the interobserver agreement rates …

Deliverance, Beth Odum Walker

All Theses

My work explores quilting as a vessel for storytelling, honoring the tradition of women’s craft while examining the intersection of personal and generational history. By repurposing fabric imbued with past lives and memories, I construct pieces that embody both the resilience and fragility of the individuals in my family, capturing their struggles, aspirations, and constraints. Through abstraction, material manipulation, and narrative layering, my quilts function as both historical records and contemporary reflections, bridging the past and present through the tactile language of textile art. In describing a quilt as an object, I am including all material, patterning, orientation, scale, and …

Prevalence Of Viruses And Viroids And Seasonal Dynamics Of Prunus Necrotic Ringspot Virus And Prune Dwarf Virus In Peach Orchards In South Carolina, Garner B. Powell

All Theses

Prunus species are susceptible to plant viruses, which can cause significant losses to growers. Peach (Prunus persica [L.] Batsch) is an essential economic crop grown in the southeastern United States, specifically South Carolina and Georgia. Viruses have various mechanisms for spreading from plant to plant, including through insect vectors, pollen, pruning, seeds, and the movement of infected propagative material. Virus detection and characterization are critical to decrease virus prevalence in peach orchards. Excluding infected material is the best practice to mitigate the spread of the virus in peach orchards. Reducing virus spread is also achieved using virus-tested propagative material …

Rhetorics Of Motherhood In Fiction:Motherhood As Identity And Status-Quo, Brea A. Hudson

All Theses

Motherhood has become a highly politicized and dominant topic in public discourse, with it often being a beacon of good-will and the building block of feminine identity. Lindal Buchanan’s Rhetorics of Motherhood demonstrates how motherhood has been used by all types of political ideologies to make women more palpable in public spaces. Miranda July’s All Fours explores an identity shift in an aging mother. In my collection, I explore these different identities and facets of motherhood and what that looks like for characters who struggle with infertility, loss, and addiction. My collection shows motherhood in a realistic and messy view …

Stability Of Holes In Granular Raft, Plabon Kumar Saha

All Theses

A granular raft at an oil–water interface exhibits unique mechanical properties, including the ability to form and sustain stable holes. In this study, we experimentally investigate the formation, stability, and collapse dynamics of holes in particle rafts composed of micrometer-sized particles. Holes are created using a hollow cylinder, which generates a meniscus that inhibits particle motion, thus stabilizing the hole. Stable holes are formed when their size reaches a critical value, which depends upon the capillary length. The critical hole size does not depend upon the circularity of the hole. We show that capillary forces due to the presence of …

Characterizing Xylella Fastidiosa Pathogen Dynamics On Diverse Small Fruit Farms, Alexandra Ratay

All Theses

Global agriculture has been threatened for over a century due to the emergence of the plant pathogenic bacteria Xylella fastidiosa (Lysobacterales: Lysobacteraceae) (Xf). Xf comprises five main subspecies and induces severe physiological symptoms in a diverse set of host plant species by limiting xylem function. Xylem-feeding insect vectors can transmit Xf to host plants, spreading disease and reducing fruit yield. Here I studied Xf prevalence on small fruit farms to determine Xf host plant and vector infective status to the Xf subspecies level. I detected Xf in 28 host plants in South Carolina containing either Xf subspecies fastidiosa …

Application Of Remote Sensing In Mapping Callery Pear Distribution And Biomass Estimation In The Southeastern United States, Kavi Raj Awasthi

All Theses

Callery pear (Pyrus calleryana Decne. Rosaceae) is a rapidly spreading invasive species in the southeast United States (US) that hampers the function of native tree species and the forest ecosystem. Native to Southeast Asia, the species has encroached on the natural and secondary vegetation habitats of the Southern US in recent years. This study attempted to 1) map the Callery pear across the southeast US and 2) model the aboveground biomass (AGB) of individual Callery pear trees.

We used Callery pear locations and winter imageries of Sentinel-2, Sentinel-1, L-band radar, and terrain imagery for mapping purposes. In addition, we …

Thymosin Beta-4: A Novel Treatment For Acanthamoeba Keratitis, Victoria A. Murphey

All Theses

Acanthamoeba keratitis (AK) is a severe corneal infection caused by the free-living amoeba, Acanthamoeba. Both stages of its life cycle (trophozoite and cyst) cause infection, primarily in contact lens wearers. It is often misdiagnosed, leading to delayed treatment and poor clinical outcomes. Current treatment options for AK rely on anti-amoebic agents that are toxic to corneal epithelial cells. Further, no single drug can eliminate both forms of the amoeba. Approximately 30% of all AK infections require a corneal transplant.

Thymosin beta-4 (Tβ4) is a G-actin binding protein synthesized in almost all human cells, including corneal epithelial cells. Tβ4 exhibits …

African Sleeping Sickness: From Basic Biology To Future Directions, Riddhi R. Patel

All Theses

African Sleeping Sickness is a Neglected Tropical Disease (NTD) which poses a significant health risk to impoverished populations in 36 Sub-Saharan African countries. It is a devastating disease caused by the protozoan parasite Trypanosoma brucei, which is transmitted to mammals via the bite of infected tsetse flies. The disease manifests in two stages: the hemolymphatic stage and the meningoencephalitic stage. In the latter stage, it invades the Central Nervous System (CNS) and can be fatal if left untreated. It is caused by morphologically indistinguishable species of Trypanosoma brucei in both humans and animals, where in humans it is known as …

Anaerobic Soil Disinfestation As A Non-Chemical Management Option Against Fungi And Heterotrophic Bacteria In Organic Sweetpotato Production, Meredith Tucker

All Theses

North Carolina is the largest producer of sweetpotatoes in the United States, with South Carolina increasing farming acreage to 2,501 in 2022 compared to 1,300 in 2017. Growers producing conventional and organic sweetpotatoes are equally challenged with managing fungal pathogens due to fungicide resistance or fungicides being unavailable due to environmental regulations, leading many to shift to growing organic crops. Anaerobic soil disinfestation (ASD) is a non-chemical pre-plant management option that uses a carbon amendment, irrigation, and plasticulture to establish an anaerobic environment in soils, killing soil microbes. A greenhouse study was conducted to assess ASD on fungal pathogens and …

Novel Methods For Assessing And Prioritizing Road-Stream Crossings For Aquatic Organism Passage, Lesley E. Twiner

All Theses

Anthropogenic barriers such as dams and culverts have led to riverine habitat fragmentation and decreased fish diversity worldwide. Low-head structures such as culverts are far more abundant than large dams and may have a larger cumulative impact on river connectivity. Many efforts to inventory road crossing barriers and prioritize removal and restoration efforts do not consider temporal variation in flow. We used cameras to gain continuous water level monitoring data to quantify the relationship between precipitation events and outlet drop height. In the spring of 2024, we selected 25 culvert sites in upstate South Carolina and set up cameras to …

Gene Editing Combined With Apap Diet-Mediated Selection In A Ldlr-/- Mouse Model Of Familial Hypercholesterolemia, Pramita Suresh

All Theses

Cell-based gene therapy has garnered significant interest in treating familial hypercholesterolemia (FH), an autosomal co-dominant disorder characterized by elevated low-density lipoprotein cholesterol (LDL-C) levels. However, achieving a therapeutic threshold level of engraftment of gene-modified cells is a significant hurdle. In FH, as in most liver diseases, the transplantation of healthy gene-modified hepatocytes does not result in a selective advantage for the healthy hepatocytes over native hepatocytes for clonal expansion in the liver. In FH, as in most liver diseases, the transplantation of healthy gene-modified hepatocytes does not provide a selective advantage for the healthy hepatocytes over native hepatocytes regarding clonal …

Algebraic Properties Of Boolean Models, Harrison Fisher

All Theses

Boolean models are n-tuples of polynomial functions in n variables over the finite field of order 2. These models define finite dynamical systems which are used for modeling many different biological systems such as gene regulatory networks. These systems can be defined by updating every function synchronously, or by updating one function at a time asynchronously. In this project, we discuss a method for reverse engineering the model space of all Boolean models which fit a set of partial asynchronous data. This method is a generalization of a known method for synchronous data. In addition, we show that given the …

Chromosome Evolution Model Reveals Hidden Variation In Karyotype-Driven Speciation In Ferns, Thomas Buchloh

All Theses

Because karyotype change commonly generates reproductive isolation between diverging species, rapid karyotype evolution, like that seen in plants, may increase the total rate of diversification. However, few studies have investigated this predicted relationship. Tests of this prediction have identified a positive correlation between the rate of karyotype change (specifically whole genome duplications) and diversification, but tests have been restricted to relatively small and young clades. Fortunately, novel macroevolutionary models have recently become available to investigate patterns of karyotype evolution in large phylogenies, providing new opportunity to investigate variation in karyotype driven diversification. Ferns are one of the most karyotype rich …

Investigating Psychological Moderators Of The Effect Of A Slow-Paced Breathing Intervention On Decision-Making Performance, Anthony Alvarado

All Theses

Decision-making is a ubiquitous cognitive process that individuals engage in constantly—some decisions are inconsequential while others can have drastic implications. Identifying ways to improve decision-making, therefore, has potential to have lasting positive effects. One potential approach is slow-paced breathing interventions. While such interventions have shown promise for improving certain aspects of cognition, such as working memory, few studies have examined the effect of slow-paced breathing on decision-making performance. The current study sought to examine the effect of a brief slow-paced breathing intervention on performance on the Iowa Gambling Task (IGT), a reward-based decision-making task. Potential moderating variables such as stress, …

Tiktok Made Me Do It: #75hard, Alexis Lamere

All Theses

TikTok is one of many social media platforms that distribute entertainment videos to its users based on an interactive algorithm. It has an influential impact on the information individuals are exposed to, especially when it is a trending topic. These trending TikTok videos reach millions of viewers; one popular trend on this app is #75Hard. #75Hard is a restrictive fitness program emphasizing mental toughness, self-discipline, and strict diet and exercise routines. However, popular trends like this one can perpetuate unrealistic and unattainable goals that put people at risk for negative outcomes such as body dissatisfaction and disordered eating. The present …

Normative Variability In Retinal Nerve Fiber Layer Thickness: Does It Matter Where The Peaks Are?, Sowjanya Gowrisankaran, Ashkan Abbasi, Xubo Song, Joel Schuman, Gadi Wollstein, Bhavna Antony, Hiroshi Ishikawa

Wills Eye Hospital Papers

PURPOSE: Retinal nerve fiber layer thickness (RNFLT), a glaucoma biomarker, has a wide normative range affecting its sensitivity and specificity for abnormality detection. The interindividual RNFLT peak location variability contribution to this wide normative range has not been directly evaluated. The purpose of this study is to assess the effect of RNFLT peak normalization (PN) on normative variability.

METHODS: Circumpapillary RNFLT profiles at 1.7 mm radius from the optic nerve head (ONH) were re-sampled from optical coherence tomography (OCT) volumes (Cirrus HD-OCT, 200 × 200) obtained from one eye of 83 healthy individuals. Fovea-ONH axis (FOA) was calculated from corresponding …

Psychological Stress And Its Association With Bronchial Asthma In Saudi Arabia: A Cross-Sectional Study, Alaa A. Bugis, Faisal A. Turkestani, Mohammed E. Ahmed, Mohammed M. Alqahtani, Abdullah K. Alnafisi, Bassam O. Alamri, Alhasan M. Algobayshi

Saudi Medical Journal

ABSTRACT Objectives: To investigate the psychological stress and its association with bronchial asthma in Saudi Arabia, focusing on the mechanisms by which stress affects asthma. Methods: This cross-sectional study included 733 adults with asthma aged ≥18 years and was carried out between October 2022 and May 2023 in Saudi Arabia. Data were obtained using an online questionnaire assessing asthma severity and stress levels. Results: Cronbach’s alpha indicated that the reliability of the stress scale was 0.728 and the reliability of the asthma control tool was 0.819. Of the participants, 52% were women and 48% were men, with 48.7% aged 18-26 …

The Effects Of Income On Infant Health: Evidence From The Coal Boom And Bust, Chu Gek Chew

Dissertations and Theses Collection (Open Access)

An individual’s health at birth is an important component in human capital accumulation, posing significant implications both in the short- and long-run. I investigate how infant health outcomes were impacted during a period of successive positive and negative economic shocks led by the coal industry between the 1970s to 1990s. Using exogenous variation within counties’ pre-existing coal reserves, I instrument for the changes in economic opportunities during the coal boom and bust. I find some limited evidence that an increase in income improves infant health outcomes. The incidence of low birthweight is decreasing in earnings growth, and effects are stronger …

Environmental Influences Of Zero-Sum Thinking And Its Outcome, Edison Sora Tan

Dissertations and Theses Collection (Open Access)

Zero-sum thinking refers to a generalized mentality or a belief that one person’s gain can only come at the expense of another person’s loss. In this research, I argue that people’s zero-sum thinking is, at least in part, influenced by the interacting characteristics of the environment, namely, resource scarcity and population density. This is because resource scarcity highlights people’s resource insecurity, particularly when population density accentuates potential competition for finite resources. Consequently, zero-sum thinking reduces trust and cooperation as people reduce their commitment to potentially risky social investments. Analysis with data from a large-scale field study and ecological data provided …

Sleep Restriction And Its Effects On Mood, April M. Rummery Lamb

Undergraduate Honors Theses

Sleep plays a fundamental role in the human body being able to function effectively, in particular it plays an essential role in mood regulation. Previous research on sleep and mood has found that there is a correlation between receiving an inadequate amount of sleep and having a negative mood. However, the current field has mostly looked at the relationship between disordered sleep and mood disorders as opposed to everyday sleep patterns and daily mood. The present study aims to look at how sleep restrictions which occur naturally (not caused by sleep disorders such as insomnia) effect daily mood. To conduct …