Digital Commons Network^™

The Kentucky Microbiome Dataset, Rachel L. Washburn, Douglas C. Curl

Research Data--KGS

The Kentucky Microbiome Dataset is a continuously updated geolocated dataset which describes the distribution and diversity of microorganisms across the Commonwealth of Kentucky. Each geolocated point represents a location reported in the peer-reviewed literature where microorganisms were sampled from soil, water, geologic substrates, or clinical settings. Environmental studies include only non-host-associated, non-clinical microorganisms sampled from natural environments. Clinical studies are mapped separately to highlight locations where investigations into host-associated or human health-related microbes have occurred.

For each location, associated metadata include study information, taxonomic classifications, sample type, sampling method, latitude and longitude, sampling date, publication reference, and a direct link …

Investigation Of The Interaction Between Diethyl Phthalate And Ovalbumin Via Spectroscopic Techniques And Molecular Docking, Fu Chen, Huang Qun

Food and Machinery

［Objective］ To explore the interaction mechanism between diethyl phthalate (DEP ) and ovalbumin (OVA ) using multispectral analysis and molecular docking techniques.［Methods］ The Stern -Volmer equation is applied to study the fluorescence quenching mechanism of OVA by DEP,as well as to determine the binding constants and the number of binding sites between them.Thermodynamic parameters are used to identify the primary types of interaction forces.Based on Förster's non -radiative energy transfer theory,the energy transfer efficiency and binding distance between DEP and OVA are calculated.Molecular docking simulations are used to investigate the interaction forces between DEP and OVA.［Results］］ DEP can statically quench …

Preparation And Properties Of Rutin-Casein Complexes, Zhang Nan, Liu Lili, Yang Le, Li Sisi, Zhang Xinyuan

Food and Machinery

［Objective］ To investigate the effect of complexes produced by protein -polyphenol interaction on the bioavailability of polyphenols and proteins.［Methods］ Rutin -casein complexes are prepared using rutin and casein as raw materials.Their physicochemical and structural properties are analyzed.［Results］］ The optimal preparation conditions for the rutin -casein complexes are determined as follows:mass ratio of rutin to casein =1.89∶1.00,reaction time of 5.94 h,pH 8.5,and temperature of 50 ℃.Under such conditions,the binding degree is 40.23%.Compared with casein,the complexes exhibit a decrease in emulsifying activity index (8.47 m2/g) and emulsifying stability (9.39%).The solubility increases by 5.11%,surface hydrophobicity is significantly enhanced,and antioxidant activity markedly improves (P<0.05).Scanning electron microscopy reveals that the rutin -casein complexes display a lamellar structure with smooth surfaces,which may contribute to its functional properties.Infrared spectroscopy demonstrates peak broadening and redshifts in the amide I and II bands,and fluorescence intensity (including synchronous fluorescence ) decreases due to fluorescence quenching.It indicates that rutin alters the secondary structure and conformational arrangement of casein.［Conclusion］ Compared with the uncomplexed casein,the rutin -casein complexes are enhanced in solubility,surface hydrophobicity,and antioxidant activity.引用格式：张楠，刘丽莉，杨乐，等.芦丁—酪蛋白复合物的制备及特性研究 ［J］.食品与机械，2025，41（7）：9-17.C itation:ZHANG Nan,LIU Lili,YANG Le,et al.Preparation and properties of rutin-casein complexes [J].Food & Machinery,2025,41(7):9-17.

Ultrasound-Assisted Enzymatic Extraction And Structural Characterization Of Pectin From The Tender Leaves Of Abelia Macrotera, Mao Qiqi, Luo Yang, Wang Jianglin, Luo Jie, Fan Xiankang, Liu Chengguo, Zhou Hui

Food and Machinery

［Objective］ To clarify the physicochemical properties,microscopic structure,monosaccharide composition,relative molecular mass,and structural characteristics of pectin in the tender leaves of Abelia macrotera.［Methods］ Pectin from the tender leaves of Abelia macrotera was extracted by ultrasound -assisted cellulase method.The microscopic structure was observed under scanning electron microscope.The monosaccharide composition was determined by high performance liquid chromatography.The relative molecular mass was determined by polysaccharide hydrolysis derivatization.The possible structure was inferred by infrared spectroscopy.［Results］］ The yield of pectin from the tender leaves of Abelia macrotera is 23.38%,the esterification degree was 1.17%,and the galacturonic acid content was 72.16%.The pectin is in the form of flakes with …

Online Detection Of Tomato Internal And External Quality Based On Iot And Machine Learning, Gao Yanfei, Xu Xuefeng, Huang Yu, Zhao Xiaofei

Food and Machinery

［Objective］ To address the low efficiency and strong subjectivity of traditional manual tomato grading,this study developed an online tomato internal and external quality detection and grading system based on the Internet of Things (IoT) and machine learning technologies,enabling real -time,non -destructive detection of both internal and external quality attributes.［Methods］ By integrating machine vision and near -infrared spectroscopy,and leveraging IoT and machine learning algorithms,a comprehensive system for online,non-destructive tomato detection and grading was designed and implemented.Real -time acquisition of external images and internal spectral information of tomatoes was performed.External defects,shape index,and diameter were detected using deep learning models,while soluble solids content …

Effects Of Electron Beam Irradiation On Carotenoids And Capsaicinoids In Pepper Oils Prepared By Different Plant Oils, Ouyang Mengyun, Li Wenge, Cao Sheng, Mo Mo, Zhou Yiji, Zhang Qiling, Xu Yuanfang

Food and Machinery

［Objective］ To investigate the irradiation stability of carotenoids and capsaicinoids in pepper oils prepared using different plant oils.［Methods］ With rapeseed oil (RO),soybean oil (SO),and their blended oil (RSO ) as materials,pepper oils RO -P,SO-P,and RSO -P were prepared,and subjected to high -energy electron beam irradiation (10 MeV ).The effects of different irradiation doses (4~12 kGy ) on the peroxide value (PV),acid value (AV),carotenoid content,and capsaicinoid content of the pepper oils were investigated.［Results］］ Electron beam irradiation promoted lipid oxidation in the pepper oils,resulting in increased PV and AV values,with SO -P exhibiting the highest degree of lipid oxidation.When the irradiation dose …

Optimization Of Sulfur-Free Color Protection Process And Comparison Of Drying Techniques For Honeysuckle, Zhai Yumei, Tang Xiaojuan, Fu Xiao, Gong Min, Liu Yunguo

Food and Machinery

［Objective］ To develop green processing technology to inhibit the browning of fresh honeysuckle (Lonicera japonica Thunb ) and enhance its drying quality.［Methods］ With polyphenol oxidase relative enzyme activity and color difference values as evaluation indicators,the process of sulfur -free compound color -protecting agents (citric acid,D-sodium erythorbate,and DL-sodium malate ) is optimized through response surface methodology.Additionally,the effect of various drying methods,including vacuum freeze drying and microwave drying,on the quality after color protection is compared and analyzed.［Results］］ Citric acid demonstrates the best anti -browning effect.The process parameters are optimal when citric acid concentration is 0.105 mol/L,with D-sodium erythorbate concentration of 0.124 mol/L …

Protective Effects Of Lactobacillus Rhamnosus Cqfp 202412 On The Brain , Intestines , And Motor Functions Of Antibiotic-Damaged Mice, Cheng Bo, Li Chong, Meng Xianlu

Food and Machinery

［Objective］ To explore the effects of Lactobacillus rhamnosus CQFP 202412 on improving the intestinal barrier,liver,brain neurons,and motor function in mice through its antioxidant and anti -inflammatory activities.［Methods］ A mouse model was established by intraperitoneal injection of a mixed antibiotic solution (neomycin 5 mg/mL,vancomycin 25 mg/mL,amphotericin B 0.1 mg/mL,ampicillin 10 mg/mL,metronidazole 5 mg/mL,and lipopolysaccharide 1.5 μg/mL ),followed by intervention with L.rhamnosus CQFP 202412.After the intervention,weight -loaded swimming time and running exhaustion time were measured,along with liver index and oxidative stress/inflammatory markers in serum and brain tissue.Histopathological changes in the brain were observed by HE staining,and PCR was used to detect the …

Anti-Fatigue Activity Of Lycium Barbarum Polysaccharides, Sun Xin, Fan Shijun

Food and Machinery

［Objective］ To clarify the main components of Lycium barbarum polysaccharides that exert an anti -fatigue effect.［Methods］ Lycium barbarum polysaccharides with different relative molecular weights are prepared by water extraction,ultrafiltration membrane fractionation dialysis,and other methods.The components with good anti -fatigue activity are obtained by mice burden swimming test.They are then eluted and purified by ion exchange column and gel chromatography column to determine the relative molecular weights and monosaccharide components.The infrared spectrum is employed to analyze.［Results］］ The content of polysaccharide,protein,and uronic acid in Lycium barbarum polysaccharides with different molecular weights is 63.62%~75.63%,1.77%~2.63%,and 17.77%~24.77%,respectively.The LBP 2 has a higher component yield.The relative …

Effect Of Polygonatum Sibiricum Powder On Properties Of Dough And Quality Of Bread, Wu Kunwei, Wei Ming, Qian Senhe, Li Ziman, Xie Yan

Food and Machinery

［Objective］ To explore the application of Polygonatum sibiricum powder in bread,enhancing its health benefits.［Methods］ Polygonatum powder is added to wheat flour to study its effects on starch gelatinization,gluten structure,dough rheological properties,and bread baking quality.［Results］］ The addition of Polygonatum powder increases the gelatinization temperature of the flour,while peak viscosity and retrogradation values decrease.The gluten yield in the dough is reduced.Changes in the secondary structure of gluten proteins are observed,with an increase in the content of random coil structures.Polygonatum sibiricum powder increases dough hardness,elasticity modulus,and viscosity modulus,but it reduces the dough's fermentation volume.The baking quality of the bread is affected,with the bread …

Ultrasound-Assisted Extraction Of Total Flavonoids From Zanthoxylum Bungeanum Maxim . Leaves With Deep Eutectic Solvents And Their Antioxidant Activity Evaluation, Sun Wei, Gu Qingyi, Zhai Bingqian, Ye Run, Cai Jing, Qiao Xinrong

Food and Machinery

［Objective］ To investigate the effect of deep eutectic solvent (DES ) on the extraction rate of total flavonoids from Zanthoxylum bungeanum Maxim.leaves and analyze their antioxidant activity.［Methods］ Using Z.bungeanum leaves as raw material and total flavonoid extraction rate as the evaluation index,this study performs ultrasound -assisted extraction to screen the optimal DES system.The scavenging capacities of the extracted total flavonoids against hydroxyl radicals (·OH) and DPPH radicals are determined.［Results］］ The highest extraction rate of total flavonoids is achieved using a choline chloride -ethylene glycol DES system (ncholine chloride ∶nethylene glycol =1∶3) with 22% water content,a solid -to-liquid ratio (mZ.bungeanum powder∶Vsolvent …

Progress In The Application Of Molecularly Imprinted Electrochemical Sensors In The Detection Of Veterinary Drug Residues, Han Yanrong, Chen Yanrong, Wang Chenyu, Kuang Yishan, Zhang Bo

Food and Machinery

Veterinary drugs play a crucial role in ensuring the healthy development of the livestock industry.However,improper or excessive use of veterinary drugs may pose threats to the environment and human health.To achieve effective monitoring of veterinary drug residues,various detection technologies have emerged.Among them,molecularly imprinted electrochemical sensors (MIECSs ) combines the high recognition specificity of molecularly imprinted polymers (MIPs ) with the rapid and sensitive advantages of electrochemical analysis,becoming a powerful tools in the detection of veterinary drug residues and demonstrating great application potential.However,its transition to large -scale application still faces many challenges.This article systematically reviews the progress in the application of …

Research Progress On Nanomaterials In Food Preservation And Packaging, Zhou Huang, Peng Weifu, Guo Qi, Zhou Ningping

Food and Machinery

Nanomaterials,distinguished by their exceptional antimicrobial properties,mechanical performance,stability,and intelligent monitoring functions,have long driven innovation in food packaging and are regarded as promising alternatives to traditional food packaging and preservation materials.This review systematically discusses the application of nanomaterials in food safety and preservation packaging,with a focus on recent advances,the current status of integrated technology applications,and the technological bottlenecks encountered.It also highlights the limitations of current research and looks ahead to the future direction of nanotechnology in food packaging.

Advances In The Application Of Machine Learning To Food Nutrition And Health, Zhou Xin, Li Dapeng

Food and Machinery

A balanced diet is fundamental to maintaining human health and preventing or reducing the risk of chronic non -communicable diseases.Efficient and accurate analysis of nutritional and functional components in food,followed by the provision of personalized nutritional guidance,is a research hotspot in the field of food nutrition and health.This article reviews the research progress on artificial intelligence technologies,such as machine learning,in the analysis of food nutritional components,functional component analysis and screening,and personalized nutrition,and highlights the pressing issues that need to be addressed in this field.

Effects Of Drying Conditions On The Forming Process , Structure , And Properties Of Konjac Glucomannan-Based Emulsion Films, Liu Yi, Zhu Jingyu, Cheng Yuhang, Yu Xiang, Jiang Fatang

Food and Machinery

［Objective］ To investigate the mechanism by which lipid distribution induced by drying conditions affects the structure and physicochemical properties of konjac glucomannan -based emulsion films.［Methods］ A film -forming emulsion composed of konjac glucomannan,pectin,and basil essential oil (K-P-OG) was prepared.The effects of different drying temperatures (25 ℃,30 ℃,35 ℃,and 40 ℃) on the physicochemical properties of K -P-OG films were examined.The droplet morphology and water distribution during the film -forming process were tracked using confocal laser scanning microscopy (CLSM ),scanning electron microscopy (SEM ),and low -field nuclear magnetic resonance (LF-NMR ).A rheometer was used to analyze the interactions among components within …

Host Plant Resistance To Stink Bugs In Commercial Soybean Varieties, Cristofer Martinez

LSU Master's Theses

Soybean, Glycine max (L.) Merr. (Fabales: Fabaceae), is a major crop in Louisiana, where stink bugs (Hemiptera: Pentatomidae) are economically important pests that reduce seed quality and yield. Among them, the redbanded stink bug, Piezodorus guildinii (Westwood), is considered the most damaging species in the southern U.S. soybean system. Recently, Euschistus quadrator (Rolston), historically a minor pest, has increased in abundance and now dominates the brown stink bug complex in Louisiana soybean fields. While P. guildinii has been widely studied, little is known about E. quadrator.

Given the increasing pest complexity, sustainable management strategies are needed to target …

Approximating Data-Driven Combinatorial Search In The Feature Space: Pwfs, Mehmet Bugrahan Ayanoglu

USF Tampa Graduate Theses and Dissertations

This dissertation presents the development and application of a novel feature selection method, Probability Weighted Feature Selection (PWFS), designed to address key challenges in machine learning involving high-dimensional, noisy, or biased datasets. It is a comprehensive exploration of feature selection strategies to enhance the performance, interpretability, and practicality of machine learning models in both engineering systems and educational data mining. PWFS introduces a structured, probabilistic approach to feature selection that adaptively weights features based on their empirical contribution to model performance. This method not only accelerates convergence to near-optimal feature subsets but also enhances flexibility for ensemble learning by encouraging …

Analyses Of Morphological Effects Of Cryopreservation On Plasmodium Yoelii Sporozoites, Lauren J. Topley

USF Tampa Graduate Theses and Dissertations

Malaria is a vector-borne disease and is a major global public health threat caused by the genus Plasmodium. Of the five species that infected humans, Plasmodium vivax and Plasmodium falciparum contribute the highest rates of morbidity and mortality. Research efforts to develop antimalarial drugs and effective vaccines is imperative to the prevention of future cases. Plasmodium has three reproductive stages: sporogonic, liver, and blood. Sporozoites are injected into the human host from an infected female Anopheles mosquito, which travel to the liver to initiate liver stage malaria. Since few sporozoites are successful, the liver stage is a bottleneck of the …

Vitamin K And Women's Health: A Review, Sharifa Alblooshi

All Works

Vitamin K is a fat-soluble vitamin essential in various physiological functions such as blood coagulation, bone metabolism, cardiovascular health, glucose regulation, immune function, neuroprotection, and vascular health. In this narrative review, studies from databases including PubMed, Google Scholar, Scopus, and the institutional database of Zayed University were selected. The role of vitamin K in women's health, with a focus on osteoporosis, postmenopausal health, cardiovascular health, diabetes, cancer, kidney health, brain health, vascular health, and pregnancy were explored. The role of vitamin K in activating vitamin K-dependent proteins is essential for many of its functions. Vitamin K-dependent proteins such as osteocalcin …

Investigation Of Subcellular Damage And Long-Term Non-Cancerous Cardiovascular Effects Following Whole-Body Photon Irradiation, Nousha Afshari

LSU Doctoral Dissertations

Current radiobiological models focus primarily on nuclear DNA damage and fail to fully explain radiation-induced noncancerous health effects. This dissertation investigates whether non-nuclear subcellular structures contribute to radiation-induced biological damage through experimental biomarker analysis and computational microdosimetry. The central hypothesis posits that whole-body photon irradiation results in subcellular non-nuclear damage, specifically increased circulating mitochondrial DNA concentrations, that correlates with radiation dose and long-term noncancerous effects such as cardiovascular disease.

Specific Aim 1 measured mitochondrial and nuclear DNA concentrations in banked blood samples from 72 Chinese rhesus macaques that previously received whole-body photon irradiation ($0-10$ Gy) from Cobalt-60 or LINAC sources. …

Cilia In The Brain Display Region-Dependent Oscillations Of Length And Orientation, Roudabeh Vakil Monfared, Sherif Abdelkarim, Pieter Derdeyn, Kiki Chen, Hanting Wu, Kenneth Leong, Tiffany Chang, Justine Lee, Sara Versales, Surya M. Nauli, Kevin Beier, Pierre Baldi, Amal Alachkar

Pharmacy Faculty Articles and Research

In this study, we conducted high-throughput spatiotemporal analysis of primary cilia length and orientation across 22 mouse brain regions. We developed automated image analysis algorithms, which enabled us to examine over 10 million individual cilia, generating the largest spatiotemporal atlas of cilia. We found that cilia length and orientation display substantial variations across different brain regions and exhibit fluctuations over a 24-h period, with region-specific peaks during light-dark phases. Our analysis revealed unique orientation patterns of cilia, suggesting that cilia orientation within the brain is not random but follows specific patterns. Using BioCycle, we identified rhythmic fluctuations in cilia length …

The U.S. Executive Order On Seabed Mining Is Consistent With International Law, James Kraska

International Law Studies

This article analyzes the legality of U.S. unilateral seabed mining authorized by Executive Order 14285, signed by President Donald Trump on April 24, 2025, permitting mineral extraction on the U.S. continental shelf and international deep seabed. Critics, including China, the European Union, and the International Seabed Authority, contend that this policy violates international law by circumventing Part XI of the United Nations Convention on the Law of the Sea (UNCLOS), which designates seabed minerals as the “common heritage of mankind” and mandates regulation through the ISA. The article asserts that, as a non-party to UNCLOS, the United States is not …

Oral Dosing Of The Nucleoside Analog Obeldesivir Is Efficacious Against Rsv Infection In African Green Monkeys, Thomas Aeschbacher, Josolyn Chan, Vincent Cutillas, Varsha Nair, Nicholas C Riola, Arya Vijjapurapu, Meghan S Vermillion, Stacey Eng, Christopher Richards, Dong Han, Jason K Perry, Subhra Chaudhuri, Szu-Wen Liu, Clarissa Martinez, Nadine Peinovich, Kai-Hui Sun, Arthur Cai, Ross Martin, Jasmine Moshiri, Charlotte Hedskog, Darius Babusis, Dustin S Siegel, Rao Kalla, Vasanthi Avadhanula, Pedro A Piedra, Kim Stobbelaar, Peter L Delputte, Caleb Marceau, Roberto Mateo, Evguenia Maiorova, Hongmei Mo, Raju Subramanian, Richard L Mackman, Tomas Cihlar, Simon P Fletcher, John P Bilello

Faculty, Staff and Students Publications

Respiratory syncytial virus (RSV) is a significant cause of morbidity and mortality in high-risk populations. Although prophylactic options are available, there are no effective oral therapeutics for RSV infection. Obeldesivir (ODV) is an orally bioavailable prodrug of the nucleoside analog GS-441524, which is converted intracellularly to its active nucleoside triphosphate and inhibits the RSV RNA polymerase. Here we report the potent antiviral activity of ODV against geographically and temporally diverse RSV A and B clinical isolates (EC50: 0.20–0.66 μM). Resistance selection studies with ODV and GS-441524 against RSV identify a single amino acid substitution, I777L, in the L polymerase with …

Determination Of Vitamin B12 In Infant Formula Milk Powder By Cyanide-Free Degradation Coupled With Hydrophilic Interaction Liquid Chromatography With Fluorescence Detection, Li Liang, Deng Wenjuan, Huang Weiqian, Wu Junfa, Qin Tianfu

Food and Machinery

［Objective］ To establish a method for determining the total vitamin B12 content in infant formula milk powder based on the indirect detection of α-ribazole,a degradation product and nucleoside component of vitamin B12.［Methods］ After protein denaturation and sugar removal,the sample underwent acid hydrolysis and enzymatic dephosphorylation to release α-ribazole,which was then purified using a boronate affinity column.Quantitative analysis was performed using hydrophilic interaction liquid chromatography with fluorescence detection (HILIC -FLD ).［Results］］ Alpha -ribazole showed good linearity in the range of 1.00~10.0 μg/L,with a correlation coefficient (R²) of 0.999 1.The spiked recovery ranged from 72.5% to 78.3%,with relative standard deviations (RSDs ) …

Preparation And Performance Of Visual Active Film By Purple Yam, Deng Fuyuan, Xie Wei, Li Xin, Wen Yanqing, Zhu Guiting

Food and Machinery

［Objective］ This study aimed to develop a visual active film using the inherent anthocyanins in purple yam as a natural indicator and its rich content of starch,polysaccharides,and proteins as the film -forming matrix,providing an intuitive and effective new approach for quality control of fish products.［Methods］ Purple yam was processed by raw,steamed,and boiled treatments,then freeze -dried to obtain three types of purple yam powders,which were used to prepare visual active films corresponding to each treatment method.The effects of different citric acid additions on film properties were investigated.Based on comprehensive analysis of film characteristics,the optimal active film was selected and applied for …

Antioxidant Activity And Molecular Mechanisms Of Typical Flavonoids In Dendrobium Officinale, Wang Yixue, Qin Si, Zhou Runze, Lyu Chenghao

Food and Machinery

［Objective］ To explore the antioxidant activity and molecular mechanism of the typical flavonoid apigenin and its glycoside compounds in Dendrobium officinale.［Methods］ The antioxidant activities of apigenin and its glycoside compounds were assessed using the DPPH free radical scavenging assay,and their effects on the expression of antioxidant proteins in HepG 2 cells were examined by Western blot.［Results］］ Vitexin exhibited significantly stronger antioxidant activity than apigenin and New Zealand vitexin Ⅱ,with an IC50 value for DPPH radical scavenging of 43.48 mmol/L.MTT assays confirmed that apigenin (20 μmol/L ),vitexin,and New Zealand vitexin Ⅱ (80 μmol/L ) were non -toxic to HepG 2 cells.In …

Synergistic Depolymerization Of Polymeric Proanthocyanidins From Vitis Amurensis Seeds By Catechin And Sulfurous Acid And Their Antioxidant Activity, Lan Jing, Ren Xiangyun, Su Hao, Pang Jie, Li Weixin

Food and Machinery

［Objective］ To address the problem of low bioavailability of polymeric proanthocyanidins (PPCs ).［Methods］ PPCs extracted,isolated,and purified from Vitis amurensis seeds were used as the raw materials.The average degree of polymerization (mDP ) was used as the evaluation index.Catechin (C) combined with sulfurous acid was employed as the depolymerization reagent.The effects of the catechin -to-PPC mass ratio (mC∶mPPC),sulfurous acid amount,reaction time,and temperature on mDP were studied.The depolymerization process was optimized using response surface methodology,and the antioxidant activity of PPC before and after depolymerization was analyzed.［Results］］ Catechin combined with sulfurous acid effectively depolymerized PPC from V.amurensis seeds.The optimal depolymerization conditions were as …

Autism And Down Syndrome: An Examination Of The Baby And Infant Screen For Autism Traits In Toddlers With Down Syndrome, Celeste Tevis

LSU Doctoral Dissertations

Current research suggests that children with Down Syndrome (DS) are at an increased risk for autism spectrum disorder (ASD) compared to the general population. Detecting ASD early in children with DS is crucial to providing access to additional treatment services and supports that may be necessary. Despite a critical need to identify ASD as early as possible, diagnoses of ASD are often delayed in individuals with DS. It can be especially difficult for clinicians to differentiate between symptoms attributed to intellectual developmental disorder (IDD), a primary feature of DS, and possible ASD symptoms. Only two research studies have evaluated the …

Molecular Epidemiology And Clinical Characterization Of Carbapenemase-Producing Enterobacter Species From An International Cohort, Blake Hanson, Scott Evans, Yohei Doi, Michael J Satlin, Gregory Weston, Eric Cober, Sandra Liliana Valderrama-Beltran, Soraya Salcedo Mendoza, Zhengyin Liu, Bettina C Fries, Paul Ananth Tambyah, Henry F Chambers, Vance G Fowler, David Van Duin, Barry N Kreiswirth, Liang Chen

Faculty, Staff and Student Publications

Background: Despite the global public health threat posed by carbapenem-resistant Enterobacter spp, clinical and molecular epidemiological studies on international isolates remain scarce. Historically, the taxonomy of Enterobacter has been challenging, limiting our understanding of the clinical characteristics and outcomes of carbapenemase-producing Enterobacter spp infections.

Methods: Hospitalized patients enrolled in the CRACKLE-2 study (ClinicalTrials.gov, NCT03646227) from 2016 to 2018 with cultures positive for carbapenemase-producing Enterobacter spp were included. Clinical and microbiologic data were collected from health records. Whole genome sequencing was performed, and the population structures of selected predominant clones were analyzed.

Results: We enrolled 136 hospitalized patients with carbapenemase-producing …

Mir-302a/B/D-3p Differentially Expressed During Frontonasal Development Is Sensitive To Retinoic Acid Exposure

Faculty, Staff and Student Publications

Any failure in frontonasal development can lead to malformations at the middle facial region, such as frontonasal dysplasia, midfacial clefts, and hyper/hypotelorism. Various environmental factors influence morphogenesis through epigenetic regulations, including the action of noncoding microRNAs (miRNAs). However, it remains unclear how miRNAs are involved in the frontonasal development. In our analysis of publicly available miRNA-seq and RNA-seq datasets, we found that miR-28a-5p, miR-302a-3p, miR-302b-3p, and miR-302d-3p were differentially expressed in the frontonasal process during embryonic days 10.5 to 13.5 (E10.5–E13.5) in mice. Overexpression of these miRNAs led to a suppression of cell proliferation in cultured mouse embryonic frontonasal mesenchymal …